Cucsat.G1745 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G1745
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	AB hydrolase-1 domain-containing protein
Location	ctg1002: 1451696 .. 1453329 (-)
RNA-Seq Expression	Cucsat.G1745
Synteny	Cucsat.G1745

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTTCAAAAGTATATAATAGCAACAACGACCGAACGTAACATTTACATTGCCGCACCAAAATAGTTCCTAATTAGAACACACAACAATTAAATGTTAAAGCCGTCCATAAATAAAAAAAGAAAATTTCCCTTTCCAGTATAGACTTTCCAGTATAGTTGCCTTTAAATAGGTAACTCAACAACCATATCGCAATAGCATACAGTACTTCAGAGATAGAGAAAGCAAGCAATGAAGAATAATAATAATCTTCACTTTGTTTTGGTTCATGGGGCTGGCCATGGTGCCTGGTGTTGGTTCAAACTTCTCAGCCTTCTCCGCTCGGCCGGCCACCATGCTACTGCCATTGACCTTGCCAGCGCCGGTACCAACCCGAAAAAGTTGGACAACGTCGCATCTATAGAGGAGTATGTTGAGCCGTTGATGGAGTTGATTGAAGGTCTTCCTCTACAACAAAAAGTGGTTCTTGTTGGACATAGCTATGGTGGCTTTGCAATCTCTTTGGCTATGGAAAAATTCTCACATCGGATTCTTGTTTCTGTTTTTGTTACTGCTTATATGCCTCACTTCCTTTACTCTCCAGCTACTTTATTGCAAAAGGTAAAAATACAACACATGAATTATTTTTTAAAAAAATTCTTTGAAAAAAGAGCTTTGGTTTTTTAGTTTTTTTTTTTTTTTTTTTTAAATTTATGGTTATTTTGATTAAATTCACAGTTATTTAAGAGCCTTTCGGCGGAGACTTTGATGGATTGTGAATTTAAATTTGGCGATGATCCAGAAATGCCAACTTCTGTTGTTTATGGTCATAACTTTTTGAGACAAAAGTTATATACGAATTGTTCCCAGGAGGTGAGTTCTCACTAAACTTAAACTTTCTTCACTCGATGTTTTTTTTTCTTAATCATACGTGTTGTCTTGTTGTTAGGTAAGAGAATTAAATCTTTGACTTCTAGCTAGTTGGATATCAAAAAATTTAATCTTTTTGGTCTTTAGATTTAAAAAAAAACAAGAATGATTAAACCATGATTAGATAAGTTACCCTACATCTTTTCTTTTGAAGGTTTACAACTTTTAAGTTTAATTAATGTTTGATTCTTCTTTTATGATTATTTAGGATCTGGAGTTGGGAAAATTGCTGGTGAGGCCATTCAAGATGTTTTTTAAGGATTTATCTAAGGAATCCATTGTAACAGAAGCGAAATTTGGATCGGTTAATCGGGTTTTTGTGTTCTGTGAAGGAGATGACGTTATGGAGGGGAAATTCCAAAGGTTGATGATAGAAGAATTTCCACCAAAAGCTGTGAAATATATTTATGGAGGAGGCCATATGGTTATGCTCTCAAAACCCACACAACTTTATCAACATTTGGTTGAAGTTACTGAAAGTTTTAATTCAACAAATCAATGTAAATAATGACTTAAACTAGCTGCCCCTGAATTAATGTCCCTTTAATTACAACTTCCCTACCAATTAAAGATGGTCAAGCAATATATTTATCACAAAAAATTAATTTAAAAAAATTTCAATTGCTAAGCAAATATTTATAAATTAAAGTTGTTGTGTTGTACCTATTCGTAACCTCAGTAGTACCATTTCAAAGGAATATTATTTTTAAAAAGCTACTTTCTGAGTGGCTCTATATAAGTAATAATGTTGCACAATACTGTATTATCAAGATCTTTGCGAATGACAACCAACACTTTGGCACTATTGATGATGTTTCTTTGATCAATGACTATTATTTCCTTTAAATAATAAAAGTTGATGTACCATTAAAGAAACTTTTTATT

Coding sequence (CDS)

ATGAAGAATAATAATAATCTTCACTTTGTTTTGGTTCATGGGGCTGGCCATGGTGCCTGGTGTTGGTTCAAACTTCTCAGCCTTCTCCGCTCGGCCGGCCACCATGCTACTGCCATTGACCTTGCCAGCGCCGGTACCAACCCGAAAAAGTTGGACAACGTCGCATCTATAGAGGAGTATGTTGAGCCGTTGATGGAGTTGATTGAAGGTCTTCCTCTACAACAAAAAGTGGTTCTTGTTGGACATAGCTATGGTGGCTTTGCAATCTCTTTGGCTATGGAAAAATTCTCACATCGGATTCTTGTTTCTGTTTTTGTTACTGCTTATATGCCTCACTTCCTTTACTCTCCAGCTACTTTATTGCAAAAGTTATTTAAGAGCCTTTCGGCGGAGACTTTGATGGATTGTGAATTTAAATTTGGCGATGATCCAGAAATGCCAACTTCTGTTGTTTATGGTCATAACTTTTTGAGACAAAAGTTATATACGAATTGTTCCCAGGAGGATCTGGAGTTGGGAAAATTGCTGGTGAGGCCATTCAAGATGTTTTTTAAGGATTTATCTAAGGAATCCATTGTAACAGAAGCGAAATTTGGATCGGTTAATCGGGTTTTTGTGTTCTGTGAAGGAGATGACGTTATGGAGGGGAAATTCCAAAGGTTGATGATAGAAGAATTTCCACCAAAAGCTGTGAAATATATTTATGGAGGAGGCCATATGGTTATGCTCTCAAAACCCACACAACTTTATCAACATTTGGTTGAAGTTACTGAAAGTTTTAATTCAACAAATCAATGTAAATAA

Protein sequence

MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQEDLELGKLLVRPFKMFFKDLSKESIVTEAKFGSVNRVFVFCEGDDVMEGKFQRLMIEEFPPKAVKYIYGGGHMVMLSKPTQLYQHLVEVTESFNSTNQCK

Homology

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BLAST of Cucsat.G1745 vs. ExPASy Swiss-Prot
Match: Q8S9K8 (Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 6.8e-42
Identity = 82/162 (50.62%), Postives = 109/162 (67.28%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLMEL 67
           HFV VHG+ HGAWCWFKL + L+  GH  TAIDL  +G + ++L  V  +  Y+EPLM  
Sbjct: 21  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 80

Query: 68  IEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFKS 127
           +E LP  +KVVLVGHSYGG   SLAME+F  ++ V +F++AYMPH    PA L+Q+ F  
Sbjct: 81  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 140

Query: 128 LSAETLMDCEFKFGDDPE-MPTSVVYGHNFLRQKLYTNCSQE 169
           L     MDCEF F +  E  P+SV++G +FL++K Y+NC  E
Sbjct: 141 LPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLE 182

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BLAST of Cucsat.G1745 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.2e-35
Identity = 74/162 (45.68%), Postives = 108/162 (66.67%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLMEL 67
           HFVLVHGA HG W W+KL  LL +AGH  TA+DLA++GT+ +K++ + ++ +Y  PLMEL
Sbjct: 6   HFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLMEL 65

Query: 68  IEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFKS 127
           +E L   +KV+LVGHS GG  + LAMEK+  +I  +VF+ A+MP  +++ + +L++  + 
Sbjct: 66  MESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQYNER 125

Query: 128 LSAETLMDCEFKFGDDPEMP-TSVVYGHNFLRQKLYTNCSQE 169
             AE  +D +F     PE P TS+ +G  FL  KLY  CS E
Sbjct: 126 TPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPE 167

[Close]

BLAST of Cucsat.G1745 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 6.2e-35
Identity = 72/162 (44.44%), Postives = 107/162 (66.05%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLMEL 67
           HFVLVHG   GAW W+KL  LL SAGH  TA+DL++AG NP++LD + +  +Y EPLME+
Sbjct: 12  HFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPLMEV 71

Query: 68  IEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFKS 127
           +  +P  +KVVL+GHS+GG ++ LAME +  +I V+VF++A MP   +S     +K  + 
Sbjct: 72  MASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKYNEK 131

Query: 128 LSAETLMDCEFKFGDDPEMP-TSVVYGHNFLRQKLYTNCSQE 169
             A+ ++D +F    +PE P  S++ G  F+  K++ NCS E
Sbjct: 132 CPADMMLDSQFSTYGNPENPGMSMILGPQFMALKMFQNCSVE 173

[Close]

BLAST of Cucsat.G1745 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.1e-34
Identity = 71/156 (45.51%), Postives = 107/156 (68.59%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTN-PKKLDNVASIEEYVEPLME 67
           HFVLVHGA HGAWCW+K+  LL + GH  TA+DLA++G +  + + ++++ E+Y EPLM+
Sbjct: 9   HFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLMQ 68

Query: 68  LIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFK 127
           L+  LP  +KVVLVGHS+GG +++LAM+KF  +I VSVFVTA+MP   +SP+ + +K   
Sbjct: 69  LMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFAS 128

Query: 128 SLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLY 163
           S++ E  M  E +         SV +  +F++ +LY
Sbjct: 129 SMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLY 164

[Close]

BLAST of Cucsat.G1745 vs. ExPASy Swiss-Prot
Match: Q8S8S9 (Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.9e-32
Identity = 67/156 (42.95%), Postives = 103/156 (66.03%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTN-PKKLDNVASIEEYVEPLME 67
           HFVLVHG+ HGAWCW+K+  LL + GH  TA+DLA++G +  + + ++ + E+Y EPL +
Sbjct: 9   HFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLTK 68

Query: 68  LIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFK 127
           L+  LP  +KVVLVGHS+GG  +++AMEKF  +I V+VF+TA+MP   +SP+ +L K   
Sbjct: 69  LLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFGS 128

Query: 128 SLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLY 163
           ++  E  M  EF+         S+ +  +F++  LY
Sbjct: 129 NMPQEAWMGTEFEPYGSDNSGLSMFFSPDFMKLGLY 164

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BLAST of Cucsat.G1745 vs. NCBI nr
Match: XP_004141931.1 (methylesterase 10 [Cucumis sativus] >KGN48496.1 hypothetical protein Csa_003035 [Cucumis sativus])

HSP 1 Score: 343 bits (881), Expect = 1.11e-117
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 1   MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY 60
           MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY
Sbjct: 1   MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY 60

Query: 61  VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL 120
           VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL
Sbjct: 61  VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL 120

Query: 121 LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
           LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE
Sbjct: 121 LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168

[Close]

BLAST of Cucsat.G1745 vs. NCBI nr
Match: XP_008440235.1 (PREDICTED: methylesterase 10 [Cucumis melo] >TYK12912.1 methylesterase 10 [Cucumis melo var. makuwa])

HSP 1 Score: 321 bits (822), Expect = 1.50e-108
Identity = 156/168 (92.86%), Postives = 161/168 (95.83%), Query Frame = 0


Query: 1   MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY 60
           M NNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGT+P KLD+VASIEEY
Sbjct: 1   MNNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTDPTKLDDVASIEEY 60

Query: 61  VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL 120
           VEPLME IE LPLQQKVVLVGHSYGG +ISLAMEKF+HRILVSVFVTAYMPHFLYSPATL
Sbjct: 61  VEPLMEFIEVLPLQQKVVLVGHSYGGLSISLAMEKFAHRILVSVFVTAYMPHFLYSPATL 120

Query: 121 LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
           LQKLFKSLS ETLMDCEFKFGDDPEMPTSVVYGHNFLR+KLY NCSQE
Sbjct: 121 LQKLFKSLSVETLMDCEFKFGDDPEMPTSVVYGHNFLREKLYKNCSQE 168

[Close]

BLAST of Cucsat.G1745 vs. NCBI nr
Match: XP_038883006.1 (methylesterase 10-like [Benincasa hispida])

HSP 1 Score: 285 bits (728), Expect = 3.87e-94
Identity = 137/165 (83.03%), Postives = 146/165 (88.48%), Query Frame = 0


Query: 4   NNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEP 63
           NNN HFVLVHGAGHG WCWFKLLSLLRSAGHHAT IDLASAGT+P KLD+VASI  +V+P
Sbjct: 7   NNNPHFVLVHGAGHGGWCWFKLLSLLRSAGHHATVIDLASAGTDPTKLDDVASIHHHVQP 66

Query: 64  LMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQK 123
           LM+LIEG+P QQKVVLVGHSYGG  ISLAMEKF HRILVSVFVT YMPHF   PA +LQK
Sbjct: 67  LMDLIEGIPQQQKVVLVGHSYGGLLISLAMEKFPHRILVSVFVTGYMPHFHCPPAAVLQK 126

Query: 124 LFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
           LF SLS ET+MDCEFKFGDDPEMP SVVYGHNFLRQKLY NCS+E
Sbjct: 127 LFMSLSVETVMDCEFKFGDDPEMPNSVVYGHNFLRQKLYKNCSEE 171

[Close]

BLAST of Cucsat.G1745 vs. NCBI nr
Match: XP_023544380.1 (methylesterase 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 246 bits (629), Expect = 2.85e-79
Identity = 115/163 (70.55%), Postives = 131/163 (80.37%), Query Frame = 0


Query: 6   NLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLM 65
           N HFVLVHGAGHGAWCWFKLL+LL SAGHHATA+DL  AG +P KL+ V SI  +  PLM
Sbjct: 2   NPHFVLVHGAGHGAWCWFKLLNLLHSAGHHATALDLTGAGVDPTKLEEVTSIHHHAHPLM 61

Query: 66  ELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLF 125
             IE LP QQKV+LVGHS+GG  ISLAME F HRILV+VFVTAYMPHFL  PATL+Q+ F
Sbjct: 62  NFIEALPQQQKVILVGHSFGGLIISLAMEAFPHRILVAVFVTAYMPHFLSPPATLVQRFF 121

Query: 126 KSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
            +LS E L+DC+F FGDDPEMP SVV+GHNF  QKLY +CS +
Sbjct: 122 NTLSPENLLDCQFLFGDDPEMPNSVVFGHNFTTQKLYADCSNQ 164

[Close]

BLAST of Cucsat.G1745 vs. NCBI nr
Match: KAG6604200.1 (Methylesterase 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 246 bits (627), Expect = 5.91e-79
Identity = 114/163 (69.94%), Postives = 132/163 (80.98%), Query Frame = 0


Query: 6   NLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLM 65
           N HFVLVHGAGHG WCWFKLL+LL SAGHHATA+DL +AG +P KL+ V SI  +  PLM
Sbjct: 2   NPHFVLVHGAGHGGWCWFKLLNLLHSAGHHATALDLTAAGVDPTKLEEVTSIHHHAHPLM 61

Query: 66  ELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLF 125
           + IE LP QQKV+LVGHS+GG  ISLAME F HRILV+VFVTAYMPHFL  PATL+Q+ F
Sbjct: 62  DFIEALPQQQKVILVGHSFGGLIISLAMEAFPHRILVAVFVTAYMPHFLSPPATLVQRFF 121

Query: 126 KSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
            +LS + L+DC+F FGDDPEMP SVV+GHNF  QKLY +CS E
Sbjct: 122 NTLSPQNLLDCQFLFGDDPEMPNSVVFGHNFTTQKLYDHCSNE 164

[Close]

BLAST of Cucsat.G1745 vs. ExPASy TrEMBL
Match: A0A0A0KLA6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490110 PE=4 SV=1)

HSP 1 Score: 343 bits (881), Expect = 5.38e-118
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0


Query: 1   MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY 60
           MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY
Sbjct: 1   MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY 60

Query: 61  VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL 120
           VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL
Sbjct: 61  VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL 120

Query: 121 LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
           LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE
Sbjct: 121 LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168

[Close]

BLAST of Cucsat.G1745 vs. ExPASy TrEMBL
Match: A0A5D3CNI0 (Methylesterase 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005070 PE=4 SV=1)

HSP 1 Score: 321 bits (822), Expect = 7.27e-109
Identity = 156/168 (92.86%), Postives = 161/168 (95.83%), Query Frame = 0


Query: 1   MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY 60
           M NNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGT+P KLD+VASIEEY
Sbjct: 1   MNNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTDPTKLDDVASIEEY 60

Query: 61  VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL 120
           VEPLME IE LPLQQKVVLVGHSYGG +ISLAMEKF+HRILVSVFVTAYMPHFLYSPATL
Sbjct: 61  VEPLMEFIEVLPLQQKVVLVGHSYGGLSISLAMEKFAHRILVSVFVTAYMPHFLYSPATL 120

Query: 121 LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
           LQKLFKSLS ETLMDCEFKFGDDPEMPTSVVYGHNFLR+KLY NCSQE
Sbjct: 121 LQKLFKSLSVETLMDCEFKFGDDPEMPTSVVYGHNFLREKLYKNCSQE 168

[Close]

BLAST of Cucsat.G1745 vs. ExPASy TrEMBL
Match: A0A1S3B195 (methylesterase 10 OS=Cucumis melo OX=3656 GN=LOC103484750 PE=4 SV=1)

HSP 1 Score: 321 bits (822), Expect = 7.27e-109
Identity = 156/168 (92.86%), Postives = 161/168 (95.83%), Query Frame = 0


Query: 1   MKNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEY 60
           M NNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGT+P KLD+VASIEEY
Sbjct: 1   MNNNNNLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTDPTKLDDVASIEEY 60

Query: 61  VEPLMELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATL 120
           VEPLME IE LPLQQKVVLVGHSYGG +ISLAMEKF+HRILVSVFVTAYMPHFLYSPATL
Sbjct: 61  VEPLMEFIEVLPLQQKVVLVGHSYGGLSISLAMEKFAHRILVSVFVTAYMPHFLYSPATL 120

Query: 121 LQKLFKSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
           LQKLFKSLS ETLMDCEFKFGDDPEMPTSVVYGHNFLR+KLY NCSQE
Sbjct: 121 LQKLFKSLSVETLMDCEFKFGDDPEMPTSVVYGHNFLREKLYKNCSQE 168

[Close]

BLAST of Cucsat.G1745 vs. ExPASy TrEMBL
Match: A0A6J1GD10 (methylesterase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111453077 PE=4 SV=1)

HSP 1 Score: 245 bits (626), Expect = 3.81e-79
Identity = 114/163 (69.94%), Postives = 131/163 (80.37%), Query Frame = 0


Query: 6   NLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLM 65
           N HFVLVHGAGHG WCWFKLL+LL SAGHHATA+DL  AG +P KL+ V SI  +  PLM
Sbjct: 2   NPHFVLVHGAGHGGWCWFKLLNLLHSAGHHATALDLTGAGVDPTKLEEVTSIHHHAHPLM 61

Query: 66  ELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLF 125
           + IE LP QQKV+LVGHS+GG  ISLAME F HRILV+VFVTAYMPHFL  PATL+Q+ F
Sbjct: 62  DFIEALPQQQKVILVGHSFGGLIISLAMEAFPHRILVAVFVTAYMPHFLSPPATLVQRFF 121

Query: 126 KSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
            +LS + L+DC+F FGDDPEMP SVV+GHNF  QKLY +CS E
Sbjct: 122 NTLSPQNLLDCQFLFGDDPEMPNSVVFGHNFTTQKLYDHCSNE 164

[Close]

BLAST of Cucsat.G1745 vs. ExPASy TrEMBL
Match: A0A6J1IVU0 (methylesterase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111478856 PE=4 SV=1)

HSP 1 Score: 245 bits (625), Expect = 5.76e-79
Identity = 114/163 (69.94%), Postives = 133/163 (81.60%), Query Frame = 0


Query: 6   NLHFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLM 65
           N HFVLVHGAGHG WCWFKLL+LL SA +HATA+DL  AG +P KL+ V SI  + +PLM
Sbjct: 2   NPHFVLVHGAGHGGWCWFKLLNLLHSAAYHATALDLTGAGVDPTKLEEVTSIHHHAQPLM 61

Query: 66  ELIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLF 125
           + IE LP QQKV+LVGHS+GG  ISLAME F HRILV++FVTAYMPHFL  PATL+Q+ F
Sbjct: 62  DFIEALPQQQKVILVGHSFGGLIISLAMEAFPHRILVALFVTAYMPHFLSPPATLVQRFF 121

Query: 126 KSLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLYTNCSQE 168
           KSLS E L+DC+F FGDDPEMP+SVV+GHNF  QKLY +CS E
Sbjct: 122 KSLSPENLLDCQFLFGDDPEMPSSVVFGHNFTTQKLYDDCSNE 164

[Close]

BLAST of Cucsat.G1745 vs. TAIR 10
Match: AT3G50440.1 (methyl esterase 10 )

HSP 1 Score: 171.8 bits (434), Expect = 4.8e-43
Identity = 82/162 (50.62%), Postives = 109/162 (67.28%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLMEL 67
           HFV VHG+ HGAWCWFKL + L+  GH  TAIDL  +G + ++L  V  +  Y+EPLM  
Sbjct: 34  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 93

Query: 68  IEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFKS 127
           +E LP  +KVVLVGHSYGG   SLAME+F  ++ V +F++AYMPH    PA L+Q+ F  
Sbjct: 94  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 153

Query: 128 LSAETLMDCEFKFGDDPE-MPTSVVYGHNFLRQKLYTNCSQE 169
           L     MDCEF F +  E  P+SV++G +FL++K Y+NC  E
Sbjct: 154 LPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLE 195

[Close]

BLAST of Cucsat.G1745 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 147.9 bits (372), Expect = 7.5e-36
Identity = 71/156 (45.51%), Postives = 107/156 (68.59%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTN-PKKLDNVASIEEYVEPLME 67
           HFVLVHGA HGAWCW+K+  LL + GH  TA+DLA++G +  + + ++++ E+Y EPLM+
Sbjct: 9   HFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLMQ 68

Query: 68  LIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFK 127
           L+  LP  +KVVLVGHS+GG +++LAM+KF  +I VSVFVTA+MP   +SP+ + +K   
Sbjct: 69  LMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFAS 128

Query: 128 SLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLY 163
           S++ E  M  E +         SV +  +F++ +LY
Sbjct: 129 SMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLY 164

[Close]

BLAST of Cucsat.G1745 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 139.8 bits (351), Expect = 2.0e-33
Identity = 67/156 (42.95%), Postives = 103/156 (66.03%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTN-PKKLDNVASIEEYVEPLME 67
           HFVLVHG+ HGAWCW+K+  LL + GH  TA+DLA++G +  + + ++ + E+Y EPL +
Sbjct: 9   HFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLTK 68

Query: 68  LIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFK 127
           L+  LP  +KVVLVGHS+GG  +++AMEKF  +I V+VF+TA+MP   +SP+ +L K   
Sbjct: 69  LLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFGS 128

Query: 128 SLSAETLMDCEFKFGDDPEMPTSVVYGHNFLRQKLY 163
           ++  E  M  EF+         S+ +  +F++  LY
Sbjct: 129 NMPQEAWMGTEFEPYGSDNSGLSMFFSPDFMKLGLY 164

[Close]

BLAST of Cucsat.G1745 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 139.4 bits (350), Expect = 2.7e-33
Identity = 70/157 (44.59%), Postives = 105/157 (66.88%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTN-PKKLDNVASIEEYVEPLME 67
           H VLVHGA HGAWCW+K+   L ++GH  TA+DLA++G +  + + ++++ E+Y EPLM+
Sbjct: 9   HVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPLMQ 68

Query: 68  LIEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFK 127
           L+  LP  +KVVLVGHS GG ++++AM+ F  +I VSVFVTA MP   +SP+ +  KL K
Sbjct: 69  LMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDKLRK 128

Query: 128 SLSAETLMDCEFKFGDDPEMPTSV-VYGHNFLRQKLY 163
             S E  +D  F   + P+ P+   ++G  F+ + LY
Sbjct: 129 ETSREEWLDTVFT-SEKPDFPSEFWIFGPEFMAKNLY 164

[Close]

BLAST of Cucsat.G1745 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 136.0 bits (341), Expect = 2.9e-32
Identity = 62/132 (46.97%), Postives = 95/132 (71.97%), Query Frame = 0


Query: 8   HFVLVHGAGHGAWCWFKLLSLLRSAGHHATAIDLASAGTNPKKLDNVASIEEYVEPLMEL 67
           HFVLVHG+  GAWCW+K+  LL ++GH  TA+DLA+ G + + + ++++ E+Y EPL++L
Sbjct: 27  HFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSEPLIQL 86

Query: 68  IEGLPLQQKVVLVGHSYGGFAISLAMEKFSHRILVSVFVTAYMPHFLYSPATLLQKLFKS 127
           +  LP  +KVVLVGHSYGG  +++AM+KF  +I VSVFVT++MP    SP+ +L+K   +
Sbjct: 87  MTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPDTKNSPSFVLEKFAST 146

Query: 128 LSAETLMDCEFK 140
           ++ E  M  E +
Sbjct: 147 MTPEDWMGSELE 158

The following BLAST results are available for this feature:

BLAST of Cucsat.G1745 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S9K8	6.8e-42	50.62	Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1	[more]
Q6RYA0	1.2e-35	45.68	Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1	[more]
Q9SE93	6.2e-35	44.44	Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=...	[more]
O80476	1.1e-34	45.51	Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1	[more]
Q8S8S9	2.9e-32	42.95	Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1	[more]

BLAST of Cucsat.G1745 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141931.1	1.11e-117	100.00	methylesterase 10 [Cucumis sativus] >KGN48496.1 hypothetical protein Csa_003035 ...	[more]
XP_008440235.1	1.50e-108	92.86	PREDICTED: methylesterase 10 [Cucumis melo] >TYK12912.1 methylesterase 10 [Cucum...	[more]
XP_038883006.1	3.87e-94	83.03	methylesterase 10-like [Benincasa hispida]	[more]
XP_023544380.1	2.85e-79	70.55	methylesterase 10-like [Cucurbita pepo subsp. pepo]	[more]
KAG6604200.1	5.91e-79	69.94	Methylesterase 10, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cucsat.G1745 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLA6	5.38e-118	100.00	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490...	[more]
A0A5D3CNI0	7.27e-109	92.86	Methylesterase 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00...	[more]
A0A1S3B195	7.27e-109	92.86	methylesterase 10 OS=Cucumis melo OX=3656 GN=LOC103484750 PE=4 SV=1	[more]
A0A6J1GD10	3.81e-79	69.94	methylesterase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111453077 PE=4 SV=1	[more]
A0A6J1IVU0	5.76e-79	69.94	methylesterase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111478856 PE=4 SV=1	[more]

BLAST of Cucsat.G1745 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50440.1	4.8e-43	50.62	methyl esterase 10	[more]
AT2G23600.1	7.5e-36	45.51	acetone-cyanohydrin lyase	[more]
AT2G23620.1	2.0e-33	42.95	methyl esterase 1	[more]
AT2G23610.1	2.7e-33	44.59	methyl esterase 3	[more]
AT2G23590.1	2.9e-32	46.97	methyl esterase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF12697	Abhydrolase_6	coord: 9..250 e-value: 4.3E-20 score: 73.2
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 6..261 e-value: 8.2E-66 score: 224.4
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 8..255
None	No IPR available	PANTHER	PTHR10992	METHYLESTERASE FAMILY MEMBER	coord: 4..258
None	No IPR available	PANTHER	PTHR10992:SF943	METHYLESTERASE 10	coord: 4..258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G1745.T1	Cucsat.G1745.T1	mRNA
Cucsat.G1745.T2	Cucsat.G1745.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009694	jasmonic acid metabolic process
biological_process	GO:0009696	salicylic acid metabolic process
molecular_function	GO:0080030	methyl indole-3-acetate esterase activity
molecular_function	GO:0080032	methyl jasmonate esterase activity
molecular_function	GO:0080031	methyl salicylate esterase activity