Cucsat.G16442 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G16442
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pectinesterase
Location	ctg2332: 55146 .. 59543 (+)
RNA-Seq Expression	Cucsat.G16442
Synteny	Cucsat.G16442

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTCATCAAAACAATTTTTACTCTCTAAACCCTTCTCTTCATTCTTTATTTAAACGTAACACTCCCCCTTTTCTCATAGATTACATTCCCATTCAAAGATCCTTTCATATTTTTCCTTCCTCTATTTCCCAAAACAAAAACAAAAAAAAAACCCTTTAAAATTCATGAACATTTAACTCTCACTCATAGATAAACTCCTCTCTTCCACACCCTTTTAACTCCTTCCACTTTCACTTTAAAATCACTATTAACAATCCAACCTTTTAATTTGAGCACTGATCGAATTGTTTTCATGATGAGGAACTTCTTGTGTTTCCTATGTAATAACATGAATAATGGAGGGTTTTTCCCTTGCATCATCGTTCTTGGTTTTCTTCTGCTGTGTGTCGCTGGAGGGAGCAGCGGTAGGGGTCGTGGTGGTGTGATCAGTTGGGATGATCTGCGTGTGGATGTTTGGCGGCGGCAGAGGATGAGCTCCAGCGAGGGGTTGGGGAATGGGACCAGAATCATTGTGGTCGATAAAAATTGCTCCGGCGACTCCACCACGGTGCAAGGGGCGGTGGATATGGTTCCTCATAATAACAAACAGAGAGTTAAGATTTATATTCTTCCTGGCATTTACAGGTTCGTGTTGTATATAATTATACATATCATCAATTATATTTTGCCTTTTTTTTTTCTTTCTCTTTTTTAATTTCTTTTACAGTGCATATTACAAATCACATATGCAAACATCAAAATTAAAATTATTGTGTACATTATCAGATATCATCTTTAGATATTAGATATTAATTTGTTGTTTTTCTATTAAAAAAGAAAGAAAGAAAGAACTACCCATGATGAATTTTTTGTTTTGTTTGGAAAATACGACATAAAGTTAGATTATTATACTTAACTTTCTATAGATATTTTTAAGTTATCTTTTGAGTAAAAATCTTTTAAACTTTTTTTAACAAAAGTGAAAAATTGAAAAATATTTAGTGCAGTGATATCTAAAACGAGAATTTAGATTTTAGCATTATTTTAGGGTTGTTATCTCAACGTTACAAGTCTCAATTTTCGTCCAAAAATCTTTTGGAAATATTTACAATAAAAGTTAATAAATAAATAAATAACCCTAACAGTTTTAAATTATTAAGGATTATATATATATATATATGATGATTATAGGTGGTTTTCTTTTAATAACAGCATTGTAGGTGACCTTTAAAGATTGAAGTATATTATATAGACATTAGTTATGGTATACTTTAGACATTGATAAAAGTTAAATGAGAAACAAACACATAATATTAAAGTAATTGAATACGTTAGAAATCACTTGTAAAATGCTTTGTTATTTCAGATAAATAAATAAATTGGTTCATCCTAGAAAATAGAAATCGTCGAAAGACTTATATTTGCTAAAATTTAACGGTTCATTTTTATTTTATTTTTTTTTAGATAAACAATAAACAGAAATGAAACAATTATTTGTAAATATTGTTTGTTTGTTTGTTGTCATTTGAAAAGAGGTGAGGTTAATTAGGTTTCTTTTTGGAAAGATTAATTCAGGTTATATCTAAGGTGTGATAGTAACAAAAAAATCTTTTAATTTTGATAAGTTGATAAGTTATAAATTCTAAATAAATCAACCTAAATTTTGGATCAAATTTAGAACACACTTTGAATTTGATGTTTTGGATTCGGGATAATCATTTATATTAAGTTTAATAAAAATAAAATAAAAATGTCACCTTTAAAATGCTAATCTTTGTAGGTAGATTGTATCAAAATGTATGAGATTATGATGTTTTGAATAACTATTCCAAAATATAAAAATAATTCAAACAAAAGTGTGTGTAAGCAGGGAGATTTGTGTGAATAATGTTGGGAAGGTTAAATGGTAGAGTAGGTTCATGGTTTAGAATTAGAATTTAACTCATATTGTAAGAATTAACTATTATTAGACCAACTTTCTCTTGACCATAAGACATTTTGAGCTGTTTTTATTTTGTTAGAGAATTAACAAAATTTTGTGCAGAGAGAAGGTATACATACCCATCACCAAGCCATATATTTCCTTGATTGGAAACAAGAATAGAGTTACAGATACTGTTATTACATGGAATGACAAAGCATCAGACAAAAGTATCGACGGAGTCGAGCTCGGGACGTATAGAACAGCAACAGTTGCAATAGACTCAGACTACTTTTGTGCCACAGGAATCACATTTGAGGTTAGAACCAAAAAGAAACTATATAACTATAGGGAGAGAATAGTTTATTTAGAATAAGTCTTAATAATAAGAATAATGTTGATATTTGTAGAATACAGTTGTGGCAAAGCCAGGAGATAAGGGAAGACAAGGCGTAGCATTAAGAATAACAGGAGATAAAGCAATGTTCTATAGAGTGAAATTCTTGGGAGGACAAGATACACTTCTAGATGATTTGGGCACACATTATTACTATCAATGTCACATTCAAGGAAGTGTTGATTTCATTTTTGGTACTGCTAGATCACTCTATGAGGTACTCTCCTTTTACTATTCTGTTACTCATCTTAATTGTTTCCTTTAAACTTTTAAATTTTTGTAAATCAATCAATTTAAATCCTTTACTAAATTACGCTTTAAAATTGTCCAGGTATTTAATACTTAAAGTGTAGACTTTTTTATTTGATGTATGAATACTTTTCAAATGAAATCTTAATTGAGAGTCTAAATTGAGATGAACATTCAAGATTTAAATTAAATTGAGAGTCTAAATTGATATGAACATTCAAGATTTAAATTAAAATAATACACCAAAACTACAACTTTTTCAACTATTGAAAATATTTATAATATTTACAAATAGAAGAAAAGTTTATTTATAATAGAAAGTAAAATTTATTTGATGCGATAGACTTAGATATTTTTACTTTAAATACAGGTTTATTTATAATAGAAAGTAAAATTTTACTATTTTGTTAATATTATTTTACAAATAATTTTCCTAATTATAATAGGGTTATAAATTGACATTGATTTCTTGTCGTTAACCAACCAAAGATCGACAAAATTTCTTGTATTTTAATATATCCTAAATCAAAACTCCTAAAATATATCATAGCAGAGAGCTGATATGACAATGGTTCGAAGTACCCAAGACGATCTGTGAAACTTAAATATATCAAAGGAACTAAATTAAGACTCCTAAAATTATACTGATCGAATTGAATCTAAGTTCTAAGAAGGTCAAATTGACAAATGTAACCTTTTGATCTTTCAATCTTTGCAACCTCTCCATCTGATATTGATCTTCTCTCTTCAAAACTCAGCAATGTGTGATAACTTCTACAGCAGAGAGCTATGGAGCAATAGCAGCACATCATCGAGCCTCACCAGACGATGACACCGGTTTCTCCTTCGTACGCTGTGTGATTAATGGAAGTGGGAAAGTATACCTGGGAAGAGCGTGGGGGAACTACTCAAGAACAATATATTCTAACTGTTACATTGAAGATGTAATCAATCCATTAGGATGGAGTGACTGGAATGACCCATCAAGACAAAGGTAAAACTCAAAAGCCAACCCCACCCCACCCCCAAGACAAAAAGTACACTCCCGAATTTGGCAGACTTTCTTCAGTGGCCCACTCCGATTTTCATACAAACCAGGTGTGTTTTACGAAATATCTTTGATATGTTGGACTTTTTGGATACCATCAAAACAGGAATTGATGGTACTTCAAAAAAAAAAAACCTTACATATTAAAGGTATTTCGTAAATGCACACAATCAACCTAATCTAATACAATTTTTTTCAAATGTTATGTACACAGGACAGTAGCTTTTGGGCAATATAATTGCAGGGGCAGTGGAGCTAGCACTAAAGATTGGGTGAAATGGGCAAAGACCTTCAACTACGAGGAAGTGAGACCTTTTGTAGATAGGAAATACATCAAAGGAGAACAGTGGCTTAACTTATGAGTTAACAAGAAGAAATAACAACTTTTGACACTTCAGAACTTACACTTATTGCTTCCTTCTCAAAGACAATTACATCAACACCATAGAGTATCTACGAGCTAGCGTCTACGTTTAGAGCATTTCATAATTCTTTCAAATGGTTGTGTCTACTTCCTTTTTTGTTTACAAATTTTCTTGAAGACATGAATTTAATTATTTGATTGATTGATTGCACAGATCCTTTTATGATGAAAAAAAAGTATGGCCAATCTTATGGCTTTGTGTCCAATGATCAAAATGACCTTAAAGGATCGTACATATAACTATGACTAGCACGCCCACACATATATGTTATAACAACCACACTCAAGTTGCCAAGATGAATACTTATTTTAATATAACAATCTGTTAGACCTCCAATATTATATCACTACAGGAGGAAGGGGAATAAGTTTGTTAAG

Coding sequence (CDS)

ATGATGAGGAACTTCTTGTGTTTCCTATGTAATAACATGAATAATGGAGGGTTTTTCCCTTGCATCATCGTTCTTGGTTTTCTTCTGCTGTGTGTCGCTGGAGGGAGCAGCGGTAGGGGTCGTGGTGGTGTGATCAGTTGGGATGATCTGCGTGTGGATGTTTGGCGGCGGCAGAGGATGAGCTCCAGCGAGGGGTTGGGGAATGGGACCAGAATCATTGTGGTCGATAAAAATTGCTCCGGCGACTCCACCACGGTGCAAGGGGCGGTGGATATGGTTCCTCATAATAACAAACAGAGAGTTAAGATTTATATTCTTCCTGGCATTTACAGAGAGAAGGTATACATACCCATCACCAAGCCATATATTTCCTTGATTGGAAACAAGAATAGAGTTACAGATACTGTTATTACATGGAATGACAAAGCATCAGACAAAAGTATCGACGGAGTCGAGCTCGGGACGTATAGAACAGCAACAGTTGCAATAGACTCAGACTACTTTTGTGCCACAGGAATCACATTTGAGAATACAGTTGTGGCAAAGCCAGGAGATAAGGGAAGACAAGGCGTAGCATTAAGAATAACAGGAGATAAAGCAATGTTCTATAGAGTGAAATTCTTGGGAGGACAAGATACACTTCTAGATGATTTGGGCACACATTATTACTATCAATGTCACATTCAAGGAAGTGTTGATTTCATTTTTGGTACTGCTAGATCACTCTATGAGCAATGTGTGATAACTTCTACAGCAGAGAGCTATGGAGCAATAGCAGCACATCATCGAGCCTCACCAGACGATGACACCGGTTTCTCCTTCGTACGCTGTGTGATTAATGGAAGTGGGAAAGTATACCTGGGAAGAGCGTGGGGGAACTACTCAAGAACAATATATTCTAACTGTTACATTGAAGATGTAATCAATCCATTAGGATGGAGTGACTGGAATGACCCATCAAGACAAAGGACAGTAGCTTTTGGGCAATATAATTGCAGGGGCAGTGGAGCTAGCACTAAAGATTGGGTGAAATGGGCAAAGACCTTCAACTACGAGGAAGTGAGACCTTTTGTAGATAGGAAATACATCAAAGGAGAACAGTGGCTTAACTTATGA

Protein sequence

MMRNFLCFLCNNMNNGGFFPCIIVLGFLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMSSSEGLGNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL

Homology

[Close]

BLAST of Cucsat.G16442 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.0e-127
Identity = 221/375 (58.93%), Postives = 274/375 (73.07%), Query Frame = 0


Query: 18  FFPCIIVLGF-LLLCVAGG-----SSGRGRGGVISWDDLRV---------------DVWR 77
           F P +++L F ++LC+         +       ISW+DLRV                 W 
Sbjct: 6   FIPAVLLLCFGVMLCLKSSCALQIGNNNELKNYISWEDLRVVEDGRIERSFSIKENSNWV 65

Query: 78  RQRMSSSEGLGNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYI 137
               +++    N  R+IVVDKN  GDS TVQGAVDMVP +N QRVKI+ILPGIYREKV +
Sbjct: 66  TTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIV 125

Query: 138 PITKPYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFE 197
           P +KPYIS IGN++   DTVI+W+DKASD   DG ELGTYRTA+V+I+SD+FCAT ITFE
Sbjct: 126 PKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFE 185

Query: 198 NTVVAKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIF 257
           NTVVA+ G++GRQ VALRI GDKA+FYRV+ LG QDTL DD G+HY+YQC+IQG+VDFIF
Sbjct: 186 NTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIF 245

Query: 258 GTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSR 317
           G A+SLY+ C I STA+ YGAIAAHHR S  +DTGFSFV C I+G+G++YLGRAWGNYSR
Sbjct: 246 GNAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSR 305

Query: 318 TIYSNCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRP 372
           T+YSNC+I D+I P+GWSDW  P RQR V FG+YNCRG GA     V W+KT   +EV+P
Sbjct: 306 TVYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKP 365

[Close]

BLAST of Cucsat.G16442 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.8e-77
Identity = 138/304 (45.39%), Postives = 192/304 (63.16%), Query Frame = 0


Query: 68  NGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIG 127
           +G ++I V  N      +VQ AVD +P NN + + I I PG YREKV +P TKPYI+  G
Sbjct: 56  SGHKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKG 115

Query: 128 NKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVAK-PGDK 187
               V  T I W+D+ASD   +G +L TY+TA+V + ++YF A  I+F NT  A  PG +
Sbjct: 116 AGRDV--TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQ 175

Query: 188 GRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQC 247
           G Q VA RI+GDKA F    F G QDTL DD G HY+ +C+I+GS+DFIFG  RS+Y+ C
Sbjct: 176 GWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDC 235

Query: 248 VITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIED 307
            + S A  +G+IAAH R  P++ TGF+FV C + G+G +Y+GRA G YSR +Y+  Y + 
Sbjct: 236 ELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDA 295

Query: 308 VINPLGWSDWNDPS-RQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKG 367
           ++   GW DW+  S + +T  FG YNC G GA+    V WA+  +YE   PF+ + ++ G
Sbjct: 296 LVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNG 355

Query: 368 EQWL 370
             W+
Sbjct: 356 RHWI 357

[Close]

BLAST of Cucsat.G16442 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.2e-74
Identity = 136/301 (45.18%), Postives = 190/301 (63.12%), Query Frame = 0


Query: 72  IIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNR 131
           + V  K+  GD T +Q A+D +P  N  RV I +  G+Y+EKV IP  K +I++ G    
Sbjct: 85  LTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEG--EG 144

Query: 132 VTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTV-VAKPGDKGRQG 191
              T + W D A      G  +GTY +A+ A++S +F A  ITF NT  V  PG  G+Q 
Sbjct: 145 AEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQA 204

Query: 192 VALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITS 251
           VALR++ D A F+  + LG QDTL D LG HYY  C+I+GSVDFIFG A SLYE C + +
Sbjct: 205 VALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHA 264

Query: 252 TAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINP 311
            A+  GA+ A  R+S  +DTGFSFV+C + G+G +YLGRAWG +SR +++  Y++++I P
Sbjct: 265 IADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILP 324

Query: 312 LGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLN 371
            GW +W DPSR+ TV +GQY C G+GA+    V WA+    EE +PF+   +I G +W+ 
Sbjct: 325 RGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 383

[Close]

BLAST of Cucsat.G16442 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 255.8 bits (652), Expect = 7.7e-67
Identity = 137/309 (44.34%), Postives = 185/309 (59.87%), Query Frame = 0


Query: 70  TRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNK 129
           T  + VDKN   + TTVQ AVD V + +++R  I+I  G+Y EKV IP TKP I+L G  
Sbjct: 88  TSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQG 147

Query: 130 NRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTV-VAKPGDKGR 189
             +  T I WND A   +      GT+  ATV +    F A  I+F N   + KPGD G 
Sbjct: 148 FDI--TAIAWNDTAYSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGA 207

Query: 190 QGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVI 249
           Q VA+RI GD++ F    F G QDTL DD G HY+  C+IQGS+DFIFG A+SLY+ C I
Sbjct: 208 QAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRI 267

Query: 250 TSTAESY--------GAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYS 309
            S A           GA+ A+ R+S D+++GFSFV C I G+G V+LGRAW  YSR ++ 
Sbjct: 268 ISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFV 327

Query: 310 NCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDR 369
           +  + DVI P GW+++NDPSR  T+ +G+YNC G GA       + +  N  +V   ++ 
Sbjct: 328 STTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINT 387

[Close]

BLAST of Cucsat.G16442 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.8e-61
Identity = 122/304 (40.13%), Postives = 176/304 (57.89%), Query Frame = 0


Query: 72  IIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNR 131
           ++ VD +  G+ + VQ A+D+VP  +  +  I +  G YREKV +   K  + + G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 132 VTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTV-VAKPGDKGRQG 191
             +T I WND A           T  + +  + +  F A  I+F+N      PG+   Q 
Sbjct: 150 --NTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQA 209

Query: 192 VALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITS 251
           VALRI GD+A FY   F G QDTLLDD G H++ +C IQGS+DFIFG  RSLY+ C I S
Sbjct: 210 VALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINS 269

Query: 252 TAES-----YGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIE 311
            A+       G+I A  R S D+ +GFSFV C I+GSG++ LGRAWG Y+  ++SN Y+ 
Sbjct: 270 IAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMS 329

Query: 312 DVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKG 370
            +I P GW++W D ++++TV FG++ C G GA  K+ V + K     E   F+D  +I G
Sbjct: 330 GIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDG 385

[Close]

BLAST of Cucsat.G16442 vs. NCBI nr
Match: XP_004150299.1 (pectinesterase QRT1 [Cucumis sativus] >KGN44235.2 hypothetical protein Csa_015463 [Cucumis sativus])

HSP 1 Score: 750 bits (1937), Expect = 1.23e-273
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0


Query: 13  MNNGGFFPCIIVLGFLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMSSSEGLGNGTRI 72
           MNNGGFFPCIIVLGFLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMSSSEGLGNGTRI
Sbjct: 1   MNNGGFFPCIIVLGFLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMSSSEGLGNGTRI 60

Query: 73  IVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRV 132
           IVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRV
Sbjct: 61  IVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRV 120

Query: 133 TDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVA 192
           TDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVA
Sbjct: 121 TDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVA 180

Query: 193 LRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTA 252
           LRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTA
Sbjct: 181 LRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTA 240

Query: 253 ESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLG 312
           ESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLG
Sbjct: 241 ESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLG 300

Query: 313 WSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 371
           WSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL
Sbjct: 301 WSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 359

[Close]

BLAST of Cucsat.G16442 vs. NCBI nr
Match: XP_008463039.1 (PREDICTED: pectinesterase QRT1 [Cucumis melo])

HSP 1 Score: 712 bits (1837), Expect = 4.41e-258
Identity = 350/378 (92.59%), Postives = 360/378 (95.24%), Query Frame = 0


Query: 2   MRNFLCFLCNNM----NNGGFFPCII-VLGFLLLCVAGG-SSGRGRGG-VISWDDLRVDV 61
           MRNFLCFLCNN     NNGGFFP II VL FLLLCV  G SSGRGRGG VISWDDLRVDV
Sbjct: 1   MRNFLCFLCNNNKNNNNNGGFFPSIIIVLSFLLLCVVSGESSGRGRGGGVISWDDLRVDV 60

Query: 62  WRRQRMSSSEGLG-NGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREK 121
           W RQRMSS EGLG NGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREK
Sbjct: 61  WLRQRMSSGEGLGRNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREK 120

Query: 122 VYIPITKPYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGI 181
           +YIPITKPYISLIGNKNRVT+TVITWNDKASDK+I+GVELGTY+TATVAIDSDYFCATGI
Sbjct: 121 LYIPITKPYISLIGNKNRVTETVITWNDKASDKNINGVELGTYKTATVAIDSDYFCATGI 180

Query: 182 TFENTVVAKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVD 241
           TFENT VAKPGDKGRQGVALRITGDKA+FYRVKFLGGQDTLLDDLGTHY+YQCHI+GSVD
Sbjct: 181 TFENTNVAKPGDKGRQGVALRITGDKAVFYRVKFLGGQDTLLDDLGTHYFYQCHIRGSVD 240

Query: 242 FIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGN 301
           FIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGN
Sbjct: 241 FIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGN 300

Query: 302 YSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEE 361
           YSRTIYSNCYIEDVINPLGWSDWNDPSRQRTV FGQYNCRGSGA+TKDWV WAKTF+YEE
Sbjct: 301 YSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVVFGQYNCRGSGANTKDWVPWAKTFSYEE 360

Query: 362 VRPFVDRKYIKGEQWLNL 371
           VRPFVDRKYIKGEQWLNL
Sbjct: 361 VRPFVDRKYIKGEQWLNL 378

[Close]

BLAST of Cucsat.G16442 vs. NCBI nr
Match: XP_038896509.1 (pectinesterase QRT1 [Benincasa hispida])

HSP 1 Score: 645 bits (1664), Expect = 1.13e-231
Identity = 321/383 (83.81%), Postives = 336/383 (87.73%), Query Frame = 0


Query: 2   MRNFLCFLCNNMNNGGFFPCIIVLGFLLLCVAG------GSSGRGRGGV-------ISWD 61
           M+ FLCFLCNN  N GFFP I +L FLLL VAG      G +G G GGV       ISWD
Sbjct: 1   MKRFLCFLCNN--NHGFFPLIFILVFLLLRVAGESSISNGGAGGGGGGVTERSYGMISWD 60

Query: 62  DLRVDVWRRQRMSSSEGLGNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPG 121
           DLRVDV RRQ +SS   LGNGTRIIVVDKNCSG+STTVQGAVDM PHNNKQRVKIYILPG
Sbjct: 61  DLRVDVSRRQGISSGNELGNGTRIIVVDKNCSGNSTTVQGAVDMAPHNNKQRVKIYILPG 120

Query: 122 IYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYF 181
           IYREKVYIPITKPYISLIGNKNRVTDT+ITWNDKASDK I+GVELGTYRTATVAID+DYF
Sbjct: 121 IYREKVYIPITKPYISLIGNKNRVTDTLITWNDKASDKDINGVELGTYRTATVAIDADYF 180

Query: 182 CATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHI 241
           CATGITFENTVVAKPGDKGRQ VALRITGDKAMFYRVKF+GGQDTLLDD+GTHY+YQCHI
Sbjct: 181 CATGITFENTVVAKPGDKGRQAVALRITGDKAMFYRVKFVGGQDTLLDDMGTHYFYQCHI 240

Query: 242 QGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLG 301
           QGSVDFIFGTARSLYEQCVITSTAES GAIAAHHRASPDDDTGFSFVRCVINGSGKV LG
Sbjct: 241 QGSVDFIFGTARSLYEQCVITSTAESSGAIAAHHRASPDDDTGFSFVRCVINGSGKVLLG 300

Query: 302 RAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKT 361
           RAWG YSR IYS CYIEDVI+PLGW DWNDPSRQRTV FGQYNCRGSGA+ +      K 
Sbjct: 301 RAWGTYSRIIYSYCYIEDVIDPLGWGDWNDPSRQRTVVFGQYNCRGSGANNRRLGAMGKD 360

Query: 362 FNYEEVRPFVDRKYIKGEQWLNL 371
           F+YEEVRPFVDRKYIKGEQWLNL
Sbjct: 361 FHYEEVRPFVDRKYIKGEQWLNL 381

[Close]

BLAST of Cucsat.G16442 vs. NCBI nr
Match: XP_022975722.1 (pectinesterase QRT1 [Cucurbita maxima])

HSP 1 Score: 569 bits (1466), Expect = 1.28e-201
Identity = 280/371 (75.47%), Postives = 314/371 (84.64%), Query Frame = 0


Query: 20  PCIIVLGFLLLCVAGGSSGRGR-----------GG---VISWDDLRVDVWRRQRMSSSE- 79
           P +++L  LL  ++G +SG G            GG    ISWDDLRVDV  RQ +SS   
Sbjct: 5   PLLLLLLLLLFGLSGQASGGGAAAPAAAAFAAGGGERYAISWDDLRVDVPSRQGLSSGVH 64

Query: 80  --GL--GNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITK 139
             G+  G GTRIIVVD++ SGDSTTVQGAVD+VPHNN QRVKIYI PGIYREKVYIPITK
Sbjct: 65  GGGVQNGTGTRIIVVDRSSSGDSTTVQGAVDLVPHNNNQRVKIYIRPGIYREKVYIPITK 124

Query: 140 PYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVV 199
           PYISLIGN+ R+TDTVIT N KASDK + G+ELGTYRTATVAIDSDYFCA+GITFENTVV
Sbjct: 125 PYISLIGNRKRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVV 184

Query: 200 AKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTAR 259
           + PGDKGRQ VALRI+G+KAMFYRVKFLG QDTLLDD+GTHY+YQCHIQG++DFIFGTAR
Sbjct: 185 SAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTAR 244

Query: 260 SLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYS 319
           SLYEQCVITSTAE++GAIAAHHR+SPDDDTGFSFVRCVINGSG+V LGRAWG YSRTIYS
Sbjct: 245 SLYEQCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYS 304

Query: 320 NCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDR 371
            CYIED+I+PLGWSDW+DPSRQRT  FGQY CRGSGA+TK WV WA+TF  EEVRP+VDR
Sbjct: 305 YCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDR 364

[Close]

BLAST of Cucsat.G16442 vs. NCBI nr
Match: XP_022139683.1 (pectinesterase QRT1 [Momordica charantia])

HSP 1 Score: 567 bits (1461), Expect = 3.97e-201
Identity = 272/345 (78.84%), Postives = 302/345 (87.54%), Query Frame = 0


Query: 27  FLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMSSSEGLGNGTRIIVVDKNCSGDSTTV 86
           F+LL VAGG  GR     I WDDL +D  RRQ ++S  G   GTRIIVVDKNCSGDS TV
Sbjct: 19  FVLLGVAGG--GRTERYAIGWDDLHIDA-RRQGLNS--GDNGGTRIIVVDKNCSGDSPTV 78

Query: 87  QGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDK 146
           QGAVD+VPH NKQRVKIYILPGIYREKV IP TKPYISLIGNK RVTDT+ITWNDKASD+
Sbjct: 79  QGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDR 138

Query: 147 SIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVK 206
            I+G ELGTYRTATV+++SDYFCATGITFENTVVA+PGD GRQ VALRI GDKAMFYRVK
Sbjct: 139 DINGGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVK 198

Query: 207 FLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASP 266
           F+G QDTLLDD+GTHY+YQCHIQGSVDFIFG ARSLYEQC+I+STAE+YGAIAAHHR S 
Sbjct: 199 FIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRDSA 258

Query: 267 DDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVA 326
           +++TGFSFV CVINGSGK+ LGRAWGNYSRTIYS CYI+D+I P GWSDW DPSRQRTV 
Sbjct: 259 EEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVV 318

Query: 327 FGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 371
           FGQYNCRGSGA+T+ WV WAKTF+YEEVRPF+D K+I GEQWL+L
Sbjct: 319 FGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 358

[Close]

BLAST of Cucsat.G16442 vs. ExPASy TrEMBL
Match: A0A0A0K706 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1)

HSP 1 Score: 774 bits (1999), Expect = 3.18e-283
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0


Query: 2   MRNFLCFLCNNMNNGGFFPCIIVLGFLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMS 61
           MRNFLCFLCNNMNNGGFFPCIIVLGFLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMS
Sbjct: 1   MRNFLCFLCNNMNNGGFFPCIIVLGFLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMS 60

Query: 62  SSEGLGNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKP 121
           SSEGLGNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKP
Sbjct: 61  SSEGLGNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKP 120

Query: 122 YISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVA 181
           YISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVA
Sbjct: 121 YISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVA 180

Query: 182 KPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARS 241
           KPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARS
Sbjct: 181 KPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARS 240

Query: 242 LYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSN 301
           LYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSN
Sbjct: 241 LYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSN 300

Query: 302 CYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRK 361
           CYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRK
Sbjct: 301 CYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRK 360

Query: 362 YIKGEQWLNL 371
           YIKGEQWLNL
Sbjct: 361 YIKGEQWLNL 370

[Close]

BLAST of Cucsat.G16442 vs. ExPASy TrEMBL
Match: A0A1S3CIB1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 2.14e-258
Identity = 350/378 (92.59%), Postives = 360/378 (95.24%), Query Frame = 0


Query: 2   MRNFLCFLCNNM----NNGGFFPCII-VLGFLLLCVAGG-SSGRGRGG-VISWDDLRVDV 61
           MRNFLCFLCNN     NNGGFFP II VL FLLLCV  G SSGRGRGG VISWDDLRVDV
Sbjct: 1   MRNFLCFLCNNNKNNNNNGGFFPSIIIVLSFLLLCVVSGESSGRGRGGGVISWDDLRVDV 60

Query: 62  WRRQRMSSSEGLG-NGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREK 121
           W RQRMSS EGLG NGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREK
Sbjct: 61  WLRQRMSSGEGLGRNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREK 120

Query: 122 VYIPITKPYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGI 181
           +YIPITKPYISLIGNKNRVT+TVITWNDKASDK+I+GVELGTY+TATVAIDSDYFCATGI
Sbjct: 121 LYIPITKPYISLIGNKNRVTETVITWNDKASDKNINGVELGTYKTATVAIDSDYFCATGI 180

Query: 182 TFENTVVAKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVD 241
           TFENT VAKPGDKGRQGVALRITGDKA+FYRVKFLGGQDTLLDDLGTHY+YQCHI+GSVD
Sbjct: 181 TFENTNVAKPGDKGRQGVALRITGDKAVFYRVKFLGGQDTLLDDLGTHYFYQCHIRGSVD 240

Query: 242 FIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGN 301
           FIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGN
Sbjct: 241 FIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGN 300

Query: 302 YSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEE 361
           YSRTIYSNCYIEDVINPLGWSDWNDPSRQRTV FGQYNCRGSGA+TKDWV WAKTF+YEE
Sbjct: 301 YSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVVFGQYNCRGSGANTKDWVPWAKTFSYEE 360

Query: 362 VRPFVDRKYIKGEQWLNL 371
           VRPFVDRKYIKGEQWLNL
Sbjct: 361 VRPFVDRKYIKGEQWLNL 378

[Close]

BLAST of Cucsat.G16442 vs. ExPASy TrEMBL
Match: A0A6J1IDU4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475843 PE=4 SV=1)

HSP 1 Score: 569 bits (1466), Expect = 6.20e-202
Identity = 280/371 (75.47%), Postives = 314/371 (84.64%), Query Frame = 0


Query: 20  PCIIVLGFLLLCVAGGSSGRGR-----------GG---VISWDDLRVDVWRRQRMSSSE- 79
           P +++L  LL  ++G +SG G            GG    ISWDDLRVDV  RQ +SS   
Sbjct: 5   PLLLLLLLLLFGLSGQASGGGAAAPAAAAFAAGGGERYAISWDDLRVDVPSRQGLSSGVH 64

Query: 80  --GL--GNGTRIIVVDKNCSGDSTTVQGAVDMVPHNNKQRVKIYILPGIYREKVYIPITK 139
             G+  G GTRIIVVD++ SGDSTTVQGAVD+VPHNN QRVKIYI PGIYREKVYIPITK
Sbjct: 65  GGGVQNGTGTRIIVVDRSSSGDSTTVQGAVDLVPHNNNQRVKIYIRPGIYREKVYIPITK 124

Query: 140 PYISLIGNKNRVTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVV 199
           PYISLIGN+ R+TDTVIT N KASDK + G+ELGTYRTATVAIDSDYFCA+GITFENTVV
Sbjct: 125 PYISLIGNRKRMTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVV 184

Query: 200 AKPGDKGRQGVALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTAR 259
           + PGDKGRQ VALRI+G+KAMFYRVKFLG QDTLLDD+GTHY+YQCHIQG++DFIFGTAR
Sbjct: 185 SAPGDKGRQAVALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTAR 244

Query: 260 SLYEQCVITSTAESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYS 319
           SLYEQCVITSTAE++GAIAAHHR+SPDDDTGFSFVRCVINGSG+V LGRAWG YSRTIYS
Sbjct: 245 SLYEQCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYS 304

Query: 320 NCYIEDVINPLGWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDR 371
            CYIED+I+PLGWSDW+DPSRQRT  FGQY CRGSGA+TK WV WA+TF  EEVRP+VDR
Sbjct: 305 YCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDR 364

[Close]

BLAST of Cucsat.G16442 vs. ExPASy TrEMBL
Match: A0A6J1CG92 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010530 PE=4 SV=1)

HSP 1 Score: 567 bits (1461), Expect = 1.92e-201
Identity = 272/345 (78.84%), Postives = 302/345 (87.54%), Query Frame = 0


Query: 27  FLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMSSSEGLGNGTRIIVVDKNCSGDSTTV 86
           F+LL VAGG  GR     I WDDL +D  RRQ ++S  G   GTRIIVVDKNCSGDS TV
Sbjct: 19  FVLLGVAGG--GRTERYAIGWDDLHIDA-RRQGLNS--GDNGGTRIIVVDKNCSGDSPTV 78

Query: 87  QGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDK 146
           QGAVD+VPH NKQRVKIYILPGIYREKV IP TKPYISLIGNK RVTDT+ITWNDKASD+
Sbjct: 79  QGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDR 138

Query: 147 SIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVK 206
            I+G ELGTYRTATV+++SDYFCATGITFENTVVA+PGD GRQ VALRI GDKAMFYRVK
Sbjct: 139 DINGGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVK 198

Query: 207 FLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASP 266
           F+G QDTLLDD+GTHY+YQCHIQGSVDFIFG ARSLYEQC+I+STAE+YGAIAAHHR S 
Sbjct: 199 FIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRDSA 258

Query: 267 DDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVA 326
           +++TGFSFV CVINGSGK+ LGRAWGNYSRTIYS CYI+D+I P GWSDW DPSRQRTV 
Sbjct: 259 EEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVV 318

Query: 327 FGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 371
           FGQYNCRGSGA+T+ WV WAKTF+YEEVRPF+D K+I GEQWL+L
Sbjct: 319 FGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 358

[Close]

BLAST of Cucsat.G16442 vs. ExPASy TrEMBL
Match: A0A6J1F7U7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111442894 PE=4 SV=1)

HSP 1 Score: 565 bits (1455), Expect = 2.72e-200
Identity = 272/341 (79.77%), Postives = 300/341 (87.98%), Query Frame = 0


Query: 36  SSGRGRGGVISWDDLRVDVWRRQRMSSSE---GLGNGTR--IIVVDKNCSGDSTTVQGAV 95
           ++G G    ISWDDLRVDV  RQ +SS     G+ NGTR  IIVVDK  SGDSTTVQGAV
Sbjct: 33  AAGGGDRYAISWDDLRVDVPSRQGLSSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGAV 92

Query: 96  DMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDKSIDG 155
           D+VPHNN  RVKIYI PGIYREKVYIPITKPYISLIGN+ R+TDTVIT N KASDK + G
Sbjct: 93  DLVPHNNNLRVKIYIRPGIYREKVYIPITKPYISLIGNRKRMTDTVITSNSKASDKDVSG 152

Query: 156 VELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVKFLGG 215
           +ELGTYRTATVAIDSDYFCA+GITFENTVV+ PGDKGRQ VALRI+G+KAMFYRVKFLG 
Sbjct: 153 LELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLGQ 212

Query: 216 QDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASPDDDT 275
           QDTLLDD+GTHY+YQCHIQG++DFIFGTARSLYEQCVITSTAE++GAIAAHHR+SPDDDT
Sbjct: 213 QDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPDDDT 272

Query: 276 GFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVAFGQY 335
           GFSFVRCVINGSG+V LGRAWG YSRTIYS CYIED+I+PLGWSDW+DPSRQRT  FGQY
Sbjct: 273 GFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQY 332

Query: 336 NCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 371
            CRGSGA+TK WV WA+TF  EEVRP+VDR +I GEQWLNL
Sbjct: 333 RCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 373

The following BLAST results are available for this feature:

BLAST of Cucsat.G16442 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FM79	3.0e-127	58.93	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]
Q8LPF3	4.8e-77	45.39	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q8VYZ3	2.2e-74	45.18	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
O23038	7.7e-67	44.34	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]
Q9ZQA3	4.8e-61	40.13	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]

BLAST of Cucsat.G16442 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150299.1	1.23e-273	100.00	pectinesterase QRT1 [Cucumis sativus] >KGN44235.2 hypothetical protein Csa_01546...	[more]
XP_008463039.1	4.41e-258	92.59	PREDICTED: pectinesterase QRT1 [Cucumis melo]	[more]
XP_038896509.1	1.13e-231	83.81	pectinesterase QRT1 [Benincasa hispida]	[more]
XP_022975722.1	1.28e-201	75.47	pectinesterase QRT1 [Cucurbita maxima]	[more]
XP_022139683.1	3.97e-201	78.84	pectinesterase QRT1 [Momordica charantia]	[more]

BLAST of Cucsat.G16442 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K706	3.18e-283	100.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1	[more]
A0A1S3CIB1	2.14e-258	92.59	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1	[more]
A0A6J1IDU4	6.20e-202	75.47	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475843 PE=4 SV=1	[more]
A0A6J1CG92	1.92e-201	78.84	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010530 PE=4 SV=1	[more]
A0A6J1F7U7	2.72e-200	79.77	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111442894 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..171 e-value: 5.0E-59 score: 202.2
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 1..165 e-value: 1.6E-37 score: 128.8
None	No IPR available	PANTHER	PTHR31321:SF31	PECTINESTERASE QRT1	coord: 1..171
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 1..171
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 28..37
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G16442.T1	Cucsat.G16442.T1	mRNA
Cucsat.G16442.T3	Cucsat.G16442.T3	mRNA
Cucsat.G16442.T2	Cucsat.G16442.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0030599	pectinesterase activity