Cucsat.G1604 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G1604
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Exopolygalacturonase-like
Location	ctg1000: 434279 .. 436240 (+)
RNA-Seq Expression	Cucsat.G1604
Synteny	Cucsat.G1604

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATCATTCATTGATAAAAAAAAAAAGAAAAAAAAGAAAACAAGAACCAATCAGAGTAGAAAAAAGAAAAAAATGGGATTGATATCAAACGTTGTGGCATTATTGCCATTGTTGTTATTGTTGGTGTCTAATTCTAAAGCTCAACCTGCTGCAGCTCAACCTTCAGCTCAACCAGCTCAACCTGCAGGTGGTGCTGGTGGTGTTTTCGATATCACAAAATACGGTGCCAAACCTAATGCAGATATAACTGCGGTACGAGTACTTGCATTAGTTGGACGAGAGATTTTAATTTATACAACATTTTAGTTATCAAACAAAATGATTTAATTTACAGGCTTTGACAAGTGCATGGAAGGAGGCGTGTGCATCGACCACTCCAAGTAAAGTTTTGATTCCAAAAGGAAGTTACGGATTAAGTCAATCCAACCTCAAAGGCCCTTGTAAAAGTCCTATCGAACTACAAATCGAAGGGACATTACAAGCTCCGGCCCATCCAGAAGGAGAAGGGCTACTCATTTTGGAAAATACCGATCTGTTGACAGTGTCAGGTACTGGAGTTTTGGATGGCCAAGGAAAAGCAGGTTGGGAAAAGAATGATTGCCATTTGAAGAAAGAATGCACCAAGCTTCCCATGGTAATTGCATGCTTTAATTACAACATGCTTATGTTATTAGCCTAACATCTTACGTATAATTTGACCACTACATATTTTTGCAATAATCAAACACGTTTTTAAATCAAGGGTTTTTTTTGTTTTGTTTAAAATGATCAGAATTTGAAGCTCAATTTCATCACAAATTCAATAGTAACGGGCATAACGTCATTGGATAGCAAGAATTTTCATATTAATCTTTTGGGTTGCAAGAACCTTACCTTTGACCACGTGACAATTACTGCACCGGGCAATAGTCCCAACACCGATGGAATTCACGTGAGTAGTTCAGAACAGATTAACATTCTCAATACCAATATCGCAACCGGAGATGATTGCATATCTGTGGGAGACTCCAACAAACAAGTAGCTATTAGCGATGTTACTTGCGGACCAGGACATGGAATTAGCATAGGAAGTTTGGGAAAATATACCAAGGAAAAAGAAGTGGTAGGAGTTACGGTGAAAAAATGCAAATTGACCGGTACTACAAACGGTGTTAGGATTAAAACATGGCCAGATTCTGCCGTCGCATTTCCAGCCACAGACATGCATTTTGAAGATATAGAAATGGATAATGTCAGTAACCCTATCATCATAGACCAAGAATATTGTCCATGGAACCAATGCAACCGAAAGGTATGTGCTAAAATTAGGTCTCTTTAGTTTTGAACTAATTAACTGACTCAAAAGACTGCAATAGTAATAATATTGTTCGAGTTATGTATTTTTGTTATGTTTTCTTTGTTGTTCAGGTTGCATCAAAAATTAAGATCAGCAATGTAAGCTTCAAGAATATTAGAGGCACGTCTGCAACTGCGATTGCAGTCAAAATTGTTTGCAGTAAGAGTATTCCATGTGAGGGAGTGGAAATTGCTGATATTGACCTCACTTATAGTGGACCAGAAGGTCCAATTAAGTCTCAGTGTGCAAATGTGAAACCCGTCATTACAGGAAAACAAATCCTCCAATCTGTGGTGAAGTTGCACCAGCTGATGGTCCATCAACCGATTGAAGTTATGAAGATGTGACAAAAGTTATTATACGCATTAAAGTTCGATTACATGCATTTTTGTGATTATAGGCATGCTCTCAATAATTGTATTACGATTGTTTGGAGAGAAATAACAGTTGTCTATTTATAATTTCTTCACCTCCCTCTATCGTATAACCTAATTAAAAGAAAAAGCAAAAAAAAAATGTTTTACTTCGGTTTACATTGTATTTTTGGACTTGAAATTTTCATCCCTAGTGTTAAAATTTTGTGCGTGAAAGAAAATTTTAAATGTCAAAATTGTTGAAAACATATAC

Coding sequence (CDS)

TCATTCATTGATAAAAAAAAAAAGAAAAAAAAGAAAACAAGAACCAATCAGAGTAGAAAAAAGAAAAAAATGGGATTGATATCAAACGTTGTGGCATTATTGCCATTGTTGTTATTGTTGGTGTCTAATTCTAAAGCTCAACCTGCTGCAGCTCAACCTTCAGCTCAACCAGCTCAACCTGCAGGTGGTGCTGGTGGTGTTTTCGATATCACAAAATACGGTGCCAAACCTAATGCAGATATAACTGCGGCTTTGACAAGTGCATGGAAGGAGGCGTGTGCATCGACCACTCCAAGTAAAGTTTTGATTCCAAAAGGAAGTTACGGATTAAGTCAATCCAACCTCAAAGGCCCTTGTAAAAGTCCTATCGAACTACAAATCGAAGGGACATTACAAGCTCCGGCCCATCCAGAAGGAGAAGGGCTACTCATTTTGGAAAATACCGATCTGTTGACAGTGTCAGGTACTGGAGTTTTGGATGGCCAAGGAAAAGCAGGTTGGGAAAAGAATGATTGCCATTTGAAGAAAGAATGCACCAAGCTTCCCATGGTAATTGCATGCTTTAATTACAACATGCTTATGTTATTAGCCTAA

Protein sequence

SFIDKKKKKKKKTRTNQSRKKKKMGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPMVIACFNYNMLMLLA

Homology

[Close]

BLAST of Cucsat.G1604 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.2e-30
Identity = 67/121 (55.37%), Postives = 86/121 (71.07%), Query Frame = 0


Query: 66  GVFDITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIEL 125
           GVFDITKYGA  NADI+ AL +A+KEAC ST+PS ++IPKG++ ++Q  L+GPCKSP+EL
Sbjct: 25  GVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKSPLEL 84

Query: 126 QIEGTLQAPAHPE----GEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKL 183
           QI+ TL+AP+ P     GE L +    D  T+SG G+LDGQ  A WE   C   K+C KL
Sbjct: 85  QIQATLKAPSDPSQLKVGEWLTV-NKLDQFTMSGGGILDGQAAAAWE---CKQSKKCNKL 141

[Close]

BLAST of Cucsat.G1604 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.1e-26
Identity = 61/108 (56.48%), Postives = 76/108 (70.37%), Query Frame = 0


Query: 66  GVFDITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIEL 125
           GV DI+K+G KPN+DI  ALTSAW EACASTT +K++IP G+Y L+   LKGPCK+PIEL
Sbjct: 23  GVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAPIEL 82

Query: 126 QIEGTLQAPAHPE----GEGLLILENTDLLTVSGTGVLDGQGKAGWEK 170
           Q++GT+QAPA P      E        D LT+SG GV DGQG   ++K
Sbjct: 83  QVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKK 130

[Close]

BLAST of Cucsat.G1604 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-25
Identity = 55/97 (56.70%), Postives = 71/97 (73.20%), Query Frame = 0


Query: 80  DITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHP-- 139
           D T ALT+AWKEACAS +PS +L+PKG++ +    L+GPCKS I LQ++GTL+APA P  
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 140 -EGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCH 174
            +G G + L   DLLT+ G GV DGQGK+ W +NDCH
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCH 97

[Close]

BLAST of Cucsat.G1604 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.9e-25
Identity = 59/124 (47.58%), Postives = 77/124 (62.10%), Query Frame = 0


Query: 64  AGGVFDITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCK-SP 123
           +G VF++  YGAK   DI+ A+  AWK ACAS  PS VLIPKG+Y + +  ++GPCK S 
Sbjct: 6   SGSVFNVNDYGAKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSK 65

Query: 124 IELQIEGTLQAPAHP---EGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECT 183
           I  QI+G ++APA P   + +G +     D LTVSGTG LDGQG+  W KN+C     C 
Sbjct: 66  IGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCK 125

[Close]

BLAST of Cucsat.G1604 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 67/162 (41.36%), Postives = 95/162 (58.64%), Query Frame = 0


Query: 30  VVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFD-ITKYGAKPN--ADITAALT 89
           V ++  LLLL +S SK QP A                 FD + K+GAK +   D++    
Sbjct: 8   VPSMFVLLLLFISASKVQPDA-----------------FDVVAKFGAKADGKTDLSKPFL 67

Query: 90  SAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHP---EGEGLL 149
            AWKEACAS TPS V+IPKG+Y LS+ NL+GPCK+PIE+ ++GT+QAPA P   +    +
Sbjct: 68  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 127

Query: 150 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPMVI 186
              + +   + G G+ DGQG   +EKN C  ++  +KLP+ I
Sbjct: 128 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNI 152

[Close]

BLAST of Cucsat.G1604 vs. NCBI nr
Match: KAE8647377.1 (hypothetical protein Csa_004085 [Cucumis sativus])

HSP 1 Score: 336 bits (862), Expect = 9.86e-112
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 6   KKKKKKKTRTNQSRKKKKMGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAG 65
           KKKKKKKTRTNQSRKKKKMGLISN+VA+LPLLLLLVSNSKAQPAAAQPSAQPAQPAG AG
Sbjct: 14  KKKKKKKTRTNQSRKKKKMGLISNIVAILPLLLLLVSNSKAQPAAAQPSAQPAQPAGDAG 73

Query: 66  GVFDITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIEL 125
           GVFDITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIEL
Sbjct: 74  GVFDITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIEL 133

Query: 126 QIEGTLQAPAHPEGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           QIEGTLQAPAHPEGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 134 QIEGTLQAPAHPEGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 191

[Close]

BLAST of Cucsat.G1604 vs. NCBI nr
Match: XP_031742831.1 (exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 334 bits (856), Expect = 7.98e-111
Identity = 174/178 (97.75%), Postives = 176/178 (98.88%), Query Frame = 0


Query: 6   KKKKKKKTRTNQSRKKKKMGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAG 65
           KKKKKKKTRTNQSRKKKKMGLISN+VA+LPLLLLLVSNSKAQPAAAQPSAQPAQPAG AG
Sbjct: 14  KKKKKKKTRTNQSRKKKKMGLISNIVAILPLLLLLVSNSKAQPAAAQPSAQPAQPAGDAG 73

Query: 66  GVFDITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIEL 125
           GVFDITKYGAKPNADITAALTSAWK ACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIEL
Sbjct: 74  GVFDITKYGAKPNADITAALTSAWKAACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIEL 133

Query: 126 QIEGTLQAPAHPEGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           QIEGTLQAPAHPEGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 134 QIEGTLQAPAHPEGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 191

[Close]

BLAST of Cucsat.G1604 vs. NCBI nr
Match: XP_031742832.1 (exopolygalacturonase-like [Cucumis sativus] >XP_031742833.1 exopolygalacturonase-like [Cucumis sativus] >XP_031742834.1 exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 313 bits (803), Expect = 3.22e-103
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 24  MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 83
           MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA
Sbjct: 1   MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 60

Query: 84  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 143
           ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL
Sbjct: 61  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 120

Query: 144 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 121 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 160

[Close]

BLAST of Cucsat.G1604 vs. NCBI nr
Match: XP_011657634.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 312 bits (800), Expect = 9.16e-103
Identity = 158/160 (98.75%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 24  MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 83
           MGLISN+VA+LPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA
Sbjct: 1   MGLISNIVAILPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 60

Query: 84  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 143
           ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL
Sbjct: 61  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 120

Query: 144 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 121 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 160

[Close]

BLAST of Cucsat.G1604 vs. NCBI nr
Match: XP_031742970.1 (exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 311 bits (797), Expect = 2.61e-102
Identity = 158/160 (98.75%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 24  MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 83
           MGLISNVVA+LPLLLLLVSNSKAQPAAAQPSA+PAQPAGGAGGVFDITKYGAKPNADITA
Sbjct: 1   MGLISNVVAILPLLLLLVSNSKAQPAAAQPSAKPAQPAGGAGGVFDITKYGAKPNADITA 60

Query: 84  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 143
           ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL
Sbjct: 61  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 120

Query: 144 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 121 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 160

[Close]

BLAST of Cucsat.G1604 vs. ExPASy TrEMBL
Match: A0A5D3DYX7 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00040 PE=3 SV=1)

HSP 1 Score: 273 bits (697), Expect = 3.73e-91
Identity = 143/174 (82.18%), Postives = 149/174 (85.63%), Query Frame = 0


Query: 24  MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 83
           MGLISN VALL +LLLLVSNSKAQ                +GGVFDITKYGAKPNAD+TA
Sbjct: 1   MGLISNFVALLSVLLLLVSNSKAQ---------------SSGGVFDITKYGAKPNADVTA 60

Query: 84  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 143
           AL SAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKS IELQIEGTLQAPA P+G+GLL
Sbjct: 61  ALASAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLL 120

Query: 144 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPMVIACFNYNMLMLLA 197
           +LE  D LTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPMVIACFNYNM MLLA
Sbjct: 121 LLEYVDQLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPMVIACFNYNMSMLLA 159

[Close]

BLAST of Cucsat.G1604 vs. ExPASy TrEMBL
Match: A0A5A7TRZ0 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00600 PE=3 SV=1)

HSP 1 Score: 250 bits (639), Expect = 5.96e-79
Identity = 130/160 (81.25%), Postives = 136/160 (85.00%), Query Frame = 0


Query: 24  MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 83
           MGLISN V LLP+LLLLVSNSKAQ                +GGVFDITKYGAKPNAD+TA
Sbjct: 1   MGLISNFVTLLPVLLLLVSNSKAQ---------------SSGGVFDITKYGAKPNADVTA 60

Query: 84  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 143
           AL SAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKS IELQIEGTLQAPA P+G+GLL
Sbjct: 61  ALASAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLL 120

Query: 144 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           +LE  D LTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 121 LLEYVDQLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 145

[Close]

BLAST of Cucsat.G1604 vs. ExPASy TrEMBL
Match: A0A5D3DYI3 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00050 PE=3 SV=1)

HSP 1 Score: 249 bits (637), Expect = 1.19e-78
Identity = 130/160 (81.25%), Postives = 136/160 (85.00%), Query Frame = 0


Query: 24  MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 83
           MGLISN VALLP+LLLLVSNSKAQ                +G VFDITKYGAKPNAD+TA
Sbjct: 1   MGLISNFVALLPVLLLLVSNSKAQ---------------SSGSVFDITKYGAKPNADVTA 60

Query: 84  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 143
           AL SAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKS IELQIEGTLQAPA P+G+GLL
Sbjct: 61  ALVSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLL 120

Query: 144 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           +LE  D LTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 121 LLEYVDQLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 145

[Close]

BLAST of Cucsat.G1604 vs. ExPASy TrEMBL
Match: A0A5A7TR51 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00990 PE=3 SV=1)

HSP 1 Score: 249 bits (635), Expect = 2.39e-78
Identity = 130/160 (81.25%), Postives = 136/160 (85.00%), Query Frame = 0


Query: 24  MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 83
           MGLISN VALL +LLLLVSNSKAQ                +GGVFDITKYGAKPNAD+TA
Sbjct: 1   MGLISNFVALLSVLLLLVSNSKAQ---------------SSGGVFDITKYGAKPNADVTA 60

Query: 84  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 143
           AL SAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKS IELQIEGTLQAPA P+G+GLL
Sbjct: 61  ALASAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLL 120

Query: 144 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           +LE  D LTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 121 LLEYVDQLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 145

[Close]

BLAST of Cucsat.G1604 vs. ExPASy TrEMBL
Match: A0A5D3BWP4 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00550 PE=3 SV=1)

HSP 1 Score: 239 bits (610), Expect = 1.39e-74
Identity = 126/160 (78.75%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 24  MGLISNVVALLPLLLLLVSNSKAQPAAAQPSAQPAQPAGGAGGVFDITKYGAKPNADITA 83
           MGLISN VALL +LLLLVSN KAQ                +G V DITK+GAKPNAD+TA
Sbjct: 1   MGLISNFVALLHVLLLLVSNCKAQ---------------SSGVVIDITKFGAKPNADVTA 60

Query: 84  ALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIEGTLQAPAHPEGEGLL 143
           AL SAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKS IELQIEGTLQAPA P+G+GLL
Sbjct: 61  ALASAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSAIELQIEGTLQAPADPKGDGLL 120

Query: 144 ILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 183
           +LE  D LTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM
Sbjct: 121 LLEYVDQLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPM 145

[Close]

BLAST of Cucsat.G1604 vs. TAIR 10
Match: AT1G43090.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-22
Identity = 56/142 (39.44%), Postives = 82/142 (57.75%), Query Frame = 0


Query: 50  AAQPSAQPAQPAGGAGGVFDITKYGAKPN--ADITAALTSAWKEACASTT-PSKVLIPKG 109
           A++P+ +P         VF++ +YG++P+   D   A TS WK AC   +  SK+ +PKG
Sbjct: 21  ASRPTIRPK--------VFNVRRYGSRPDGKTDNANAFTSVWKHACTRISGSSKIYVPKG 80

Query: 110 SYGLSQSNLKGPCKSPIELQIEGTLQAPAHP---EGEGLLILENTDLLTVSGTGVLDGQG 169
           ++ L      GPCK+PIE  I+GTL APA+P   + +  +     + L++SG+G LDGQG
Sbjct: 81  TFYLGGVEFVGPCKNPIEFVIDGTLLAPANPRDIKQDTWINFRYINNLSISGSGTLDGQG 140

Query: 170 KAGWEKNDCHLKKECTKLPMVI 186
           K  W  NDCH    C KL M +
Sbjct: 141 KYSWPLNDCHKNTNCPKLAMTM 154

[Close]

BLAST of Cucsat.G1604 vs. TAIR 10
Match: AT1G43080.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-22
Identity = 56/142 (39.44%), Postives = 82/142 (57.75%), Query Frame = 0


Query: 50  AAQPSAQPAQPAGGAGGVFDITKYGAKPN--ADITAALTSAWKEACASTT-PSKVLIPKG 109
           A++P+ +P         VF++ +YG++P+   D   A TS WK AC   +  SK+ +PKG
Sbjct: 21  ASRPTIRPK--------VFNVRRYGSRPDGKTDNANAFTSVWKRACTRISGSSKIYVPKG 80

Query: 110 SYGLSQSNLKGPCKSPIELQIEGTLQAPAHP---EGEGLLILENTDLLTVSGTGVLDGQG 169
           ++ L      GPCK+PIE  I+GTL APA+P   + +  +     + L++SG+G LDGQG
Sbjct: 81  TFYLGGVEFVGPCKNPIEFVIDGTLLAPANPRDIKQDTWINFRYINNLSISGSGTLDGQG 140

Query: 170 KAGWEKNDCHLKKECTKLPMVI 186
           K  W  NDCH    C KL M +
Sbjct: 141 KYSWPLNDCHKNTNCPKLAMTM 154

[Close]

BLAST of Cucsat.G1604 vs. TAIR 10
Match: AT2G15460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.4e-22
Identity = 53/125 (42.40%), Postives = 74/125 (59.20%), Query Frame = 0


Query: 67  VFDITKYGAKPN--ADITAALTSAWKEACASTT-PSKVLIPKGSYGLSQSNLKGPCKSPI 126
           VF++ ++GAKP+   D   A TS W  AC   +  SK+ +PKG++ L      GPCK+PI
Sbjct: 30  VFNVQRHGAKPDGKTDNANAFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKNPI 89

Query: 127 ELQIEGTLQAPAHP---EGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTK 186
           E  I+GTL APA+P   + +  +     + L++SG+G LDGQGK  W  NDCH    C K
Sbjct: 90  EFIIDGTLLAPANPSDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPHNDCHTNPNCPK 149

[Close]

BLAST of Cucsat.G1604 vs. TAIR 10
Match: AT2G15450.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.4e-22
Identity = 53/125 (42.40%), Postives = 74/125 (59.20%), Query Frame = 0


Query: 67  VFDITKYGAKPN--ADITAALTSAWKEACASTT-PSKVLIPKGSYGLSQSNLKGPCKSPI 126
           VF++ ++GAKP+   D   A TS W  AC   +  SK+ +PKG++ L      GPCK+PI
Sbjct: 30  VFNVQRHGAKPDGKTDNANAFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKNPI 89

Query: 127 ELQIEGTLQAPAHP---EGEGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTK 186
           E  I+GTL APA+P   + +  +     + L++SG+G LDGQGK  W  NDCH    C K
Sbjct: 90  EFIIDGTLLAPANPSDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPHNDCHTNPNCPK 149

[Close]

BLAST of Cucsat.G1604 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.4e-22
Identity = 49/119 (41.18%), Postives = 76/119 (63.87%), Query Frame = 0


Query: 69  DITKYGAKPNADITAALTSAWKEACASTTPSKVLIPKGSYGLSQSNLKGPCKSPIELQIE 128
           ++   G+   +DIT AL  A+  AC S++PSKV+IPKG + L +  ++GPCK+PIE+ ++
Sbjct: 23  EVFTIGSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQ 82

Query: 129 GTLQAPAHP-EG-EGLLILENTDLLTVSGTGVLDGQGKAGWEKNDCHLKKECTKLPMVI 186
           GT++A  +  +G E  ++  N D   ++G G  DG+G A W  N+CH   EC KLP+ I
Sbjct: 83  GTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISI 141

The following BLAST results are available for this feature:

BLAST of Cucsat.G1604 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05967	5.2e-30	55.37	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
Q40312	2.1e-26	56.48	Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1	[more]
P24548	1.7e-25	56.70	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q6H9K0	3.9e-25	47.58	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
Q39766	1.1e-24	41.36	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]

BLAST of Cucsat.G1604 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647377.1	9.86e-112	98.31	hypothetical protein Csa_004085 [Cucumis sativus]	[more]
XP_031742831.1	7.98e-111	97.75	exopolygalacturonase-like [Cucumis sativus]	[more]
XP_031742832.1	3.22e-103	100.00	exopolygalacturonase-like [Cucumis sativus] >XP_031742833.1 exopolygalacturonase...	[more]
XP_011657634.1	9.16e-103	98.75	exopolygalacturonase [Cucumis sativus]	[more]
XP_031742970.1	2.61e-102	98.75	exopolygalacturonase-like [Cucumis sativus]	[more]

BLAST of Cucsat.G1604 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DYX7	3.73e-91	82.18	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7TRZ0	5.96e-79	81.25	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5D3DYI3	1.19e-78	81.25	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7TR51	2.39e-78	81.25	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A5D3BWP4	1.39e-74	78.75	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Cucsat.G1604 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G43090.1	1.4e-22	39.44	Pectin lyase-like superfamily protein	[more]
AT1G43080.1	1.4e-22	39.44	Pectin lyase-like superfamily protein	[more]
AT2G15460.1	2.4e-22	42.40	Pectin lyase-like superfamily protein	[more]
AT2G15450.1	2.4e-22	42.40	Pectin lyase-like superfamily protein	[more]
AT3G07820.1	2.4e-22	41.18	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 358..398 e-value: 5900.0 score: 1.7 coord: 240..261 e-value: 2500.0 score: 4.4 coord: 324..345 e-value: 7000.0 score: 1.2 coord: 213..239 e-value: 3.5 score: 16.6 coord: 293..314 e-value: 12.0 score: 14.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 53..445 e-value: 3.0E-107 score: 361.1
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 93..418 e-value: 5.7E-102 score: 341.1
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 270..283
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR31375:SF248	POLYGALACTURONASE	coord: 42..422
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 42..422
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 64..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G1604.T2	Cucsat.G1604.T2	mRNA
Cucsat.G1604.T1	Cucsat.G1604.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity