Cucsat.G16029 (gene) Cucumber (B10) v3

BLAST of Cucsat.G16029 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.6e-65
Identity = 132/298 (44.30%), Postives = 183/298 (61.41%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +     EYA+LP+IP YL+P+ND + +GANFAS G GA+  SH   A+GLQTQ
Sbjct: 74  RFSDGRIIPDFIAEYASLPIIPAYLEPNND-FTHGANFASAGAGALIASHAGLAVGLQTQ 133

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           + +F  +    +  LG  +S+  LS++V+LF+ GGNDY +P+   Y        QEQ+V+
Sbjct: 134 LRYFGDLVDHYRQNLGDIKSRQLLSDAVYLFSCGGNDYQSPY---YPY-----TQEQYVD 193

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFE-EASSIARIHNKFLL 188
           +V+GN+T  IK +YE GGRKFGV+ VP +G  P  R K+       E   + R+HN+   
Sbjct: 194 IVIGNMTNVIKGIYEKGGRKFGVVNVPLIGCWPGMRAKQPGNTCNTEVDELTRLHNQAFA 253

Query: 189 IALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGR--E 248
             LE+L KQL+GF Y   D+ TA+L R++NP++YGFK  ++ACCGS  F G Y+CGR  E
Sbjct: 254 KRLEQLEKQLEGFVYAKFDLSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCGRIKE 313

Query: 249 FGSSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDST 304
           FG      C N  ++ FFD FHP +   +Q A+ FW GD  + +P N K LF    ST
Sbjct: 314 FGL-----CDNATEYFFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALFEGKPST 357

BLAST of Cucsat.G16029 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 9.5e-63
Identity = 129/295 (43.73%), Postives = 179/295 (60.68%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPH--NDLYDYGANFASGGGGAIAMSHQEQAIGLQ 68
           R+  GR +     EYA LPLIPP L P   N  + YG NFASGG GA+  +     I L+
Sbjct: 78  RVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLR 137

Query: 69  TQMEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQF 128
           TQ+  F+KVE+ L++KLG A  K  +S +V+LF+ G NDY  PF  +  +F++I + E++
Sbjct: 138 TQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKY 197

Query: 129 VNMVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRL---KKSAQFFEEASSIARIHN 188
           V+ VVGN+T   KEVY  GGRKFG+L   P    P+S +    K    F+  + +  +HN
Sbjct: 198 VDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHN 257

Query: 189 KFLLIALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCG 248
           + LL  L +L+ +L GFKY   D HT+L +R+ +P++YGFK    ACCGS   RGI  CG
Sbjct: 258 EKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCG 317

Query: 249 REFG-SSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLF 298
              G S  Y  C+N+ D++FFD FH T+K  +Q+A+  WSG  +I  P N K LF
Sbjct: 318 GRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALF 371

BLAST of Cucsat.G16029 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 239.2 bits (609), Expect = 6.2e-62
Identity = 128/302 (42.38%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 2   PLSMNIFRLRHGREVLKSNGEYANLPLIPPYLDPH--NDLYDYGANFASGGGGAIAMSHQ 61
           P     F+   GR  L    E A LP IPP L P+  N+ + YG +FAS G GA+A S  
Sbjct: 64  PYGQTNFKFPTGR--LSDGPEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAESFL 123

Query: 62  EQAIGLQTQMEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKT 121
              I L TQ+  F+ VEKSL+++LG A +K   S +V+LF+ G NDY  PF  +   FK+
Sbjct: 124 GMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFKS 183

Query: 122 IEAQEQFVNMVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLK---KSAQFFEEAS 181
             ++E+FV+ V+GNIT  I+EVY+ GGRKFG L V P    P+S ++   K    F+  +
Sbjct: 184 -NSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVA 243

Query: 182 SIARIHNKFLLIALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEF 241
            +  +HNK     L +L +QL GF+Y   D HT+L +RI +P++YGFK    ACCGS   
Sbjct: 244 ELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPL 303

Query: 242 RGIYNCGREFG-SSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQ 298
           RGI  CG   G S  Y  C+N+ D++F+DS H T+K  +Q+A+  W+G  ++ +P N K 
Sbjct: 304 RGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKA 362

BLAST of Cucsat.G16029 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.4e-60
Identity = 124/295 (42.03%), Postives = 175/295 (59.32%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHN--DLYDYGANFASGGGGAIAMSHQEQAIGLQ 68
           R+  GR +     EYA LPLIP YL P N  + + YG +FAS G GA+  +     I L+
Sbjct: 80  RVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLK 139

Query: 69  TQMEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQF 128
           +Q+  F+KVEK L++ LG A+ K  +S +V+LF+ G NDY  PF  +  IF++   QE +
Sbjct: 140 SQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS-SPQEIY 199

Query: 129 VNMVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRL---KKSAQFFEEASSIARIHN 188
           V+ VVGN T  IKEVY+ GGRKFG L +      P+S +    K    F+  + +  +HN
Sbjct: 200 VDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHN 259

Query: 189 KFLLIALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCG 248
           + L   L +L ++L GFKY   D HT+L  R+ NP++YGFK    ACCG+   RGI  CG
Sbjct: 260 EKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCG 319

Query: 249 REFG-SSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLF 298
              G S  Y  C+ + D++FFD FH T+K  +Q+A+  WSG  ++ KP N + LF
Sbjct: 320 GRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALF 373

BLAST of Cucsat.G16029 vs. ExPASy Swiss-Prot
Match: Q9LJP1 (GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 5.1e-56
Identity = 119/299 (39.80%), Postives = 169/299 (56.52%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDP--HNDLYDYGANFASGGGGAIAMSHQEQAIGLQ 68
           R+  GR ++    EYA LPLIPP L P   N    YG NFA+   G  A +       L 
Sbjct: 78  RVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGTFPGSVTNLS 137

Query: 69  ----TQMEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEA 128
               TQ+  F+ VEK+L++ LG A ++  +S +V+LF+ G NDY  PF  +   F     
Sbjct: 138 KDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTSTFSN-TT 197

Query: 129 QEQFVNMVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQF---FEEASSIA 188
           +E+F++ V+GN T  I+E+Y+ G RKFG L++ P G  PS+ +  S +    FE  + + 
Sbjct: 198 KERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSCFEPVTELI 257

Query: 189 RIHNKFLLIALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGI 248
            +HN+     L +L ++L GFKY   D HT+L QRI NP+ YGFK  + ACCGS   RGI
Sbjct: 258 NLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLRGI 317

Query: 249 YNCG-REFGSSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLF 298
             CG R   S  Y  C+N +D++FFD  H T+   +Q+A+  WSG  ++  P N K LF
Sbjct: 318 NTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTLF 375

BLAST of Cucsat.G16029 vs. NCBI nr
Match: XP_004148444.1 (GDSL lipase [Cucumis sativus] >KGN62086.1 hypothetical protein Csa_006739 [Cucumis sativus])

HSP 1 Score: 592 bits (1525), Expect = 1.16e-211
Identity = 289/299 (96.66%), Postives = 291/299 (97.32%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ
Sbjct: 80  RFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 139

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           MEFFRKVEKSL+NKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN
Sbjct: 140 MEFFRKVEKSLRNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 199

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI
Sbjct: 200 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 259

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 248
           ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS
Sbjct: 260 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 319

Query: 249 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY 307
           SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY
Sbjct: 320 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY 378

BLAST of Cucsat.G16029 vs. NCBI nr
Match: XP_016902155.1 (PREDICTED: GDSL esterase/lipase 1-like [Cucumis melo] >KAA0045892.1 GDSL esterase/lipase 1-like [Cucumis melo var. makuwa] >TYK13695.1 GDSL esterase/lipase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 556 bits (1433), Expect = 1.24e-197
Identity = 271/300 (90.33%), Postives = 284/300 (94.67%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPLIPPYLDPHNDLYDYG NFASGGGGA+A+SHQEQAIGLQTQ
Sbjct: 80  RFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYGVNFASGGGGALALSHQEQAIGLQTQ 139

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           M FFRKV KSL+NKLGHARS+SFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQ+VN
Sbjct: 140 MVFFRKVVKSLRNKLGHARSRSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQYVN 199

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MVVGNITIAIKEVY+YGGRKFGVLAVPPLGYMPSSRLKKSAQFFEE+SSIARIHNKF+LI
Sbjct: 200 MVVGNITIAIKEVYKYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEESSSIARIHNKFILI 259

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGR-EFG 248
           ALEKLSKQLKGFKYTFADVHTALLQRIQNPT+YGFKVVDTACCGSDEFRG+YNCGR +FG
Sbjct: 260 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTKYGFKVVDTACCGSDEFRGVYNCGRKDFG 319

Query: 249 SSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY 307
           S PYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGD DIVKP+NFKQLF  DDSTLASY
Sbjct: 320 SLPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDVDIVKPLNFKQLFSCDDSTLASY 379

BLAST of Cucsat.G16029 vs. NCBI nr
Match: XP_038900701.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 524 bits (1349), Expect = 6.35e-185
Identity = 249/299 (83.28%), Postives = 276/299 (92.31%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPL+PPYLDPHN+LY++G NFASGGGGA+A+SHQ+QAIGLQTQ
Sbjct: 77  RFTDGRNIPDFLGEYANLPLLPPYLDPHNNLYEHGVNFASGGGGALALSHQDQAIGLQTQ 136

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           ++FF+ VEKSL+ KLG+ARSK+FLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQE +VN
Sbjct: 137 LKFFKNVEKSLRKKLGYARSKTFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEHYVN 196

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MV+GNITIA+KEVY+YGGRKFG LAVPPLGYMPSSRLKKSAQFFEEASSIARIHNK L I
Sbjct: 197 MVIGNITIAVKEVYKYGGRKFGFLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKLLPI 256

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 248
           AL+KLSKQLKGFKYTFADVHT LLQRI NPT+YGFK+VDTACCGSDEFRG+YNCGR+FG 
Sbjct: 257 ALQKLSKQLKGFKYTFADVHTLLLQRILNPTKYGFKIVDTACCGSDEFRGVYNCGRKFGD 316

Query: 249 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY 307
            PYTHC+NLEDHMFFDSFHPTQKVFKQLA+EFWSGD +IVKP+NFKQLF YD S LASY
Sbjct: 317 LPYTHCENLEDHMFFDSFHPTQKVFKQLAEEFWSGDAEIVKPLNFKQLFQYD-SNLASY 374

BLAST of Cucsat.G16029 vs. NCBI nr
Match: XP_022928027.1 (GDSL esterase/lipase 3-like [Cucurbita moschata])

HSP 1 Score: 503 bits (1295), Expect = 1.40e-176
Identity = 235/298 (78.86%), Postives = 268/298 (89.93%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPL+PPYLDPHNDLYDYG NFASGGGGA+A+SHQEQA+GLQTQ
Sbjct: 84  RFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVNFASGGGGALALSHQEQALGLQTQ 143

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           M+FF++V+KSL+ KLG+AR++SF SNSVFLFNFGGNDYLNPFDISYDIFKTI+AQEQFVN
Sbjct: 144 MKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQFVN 203

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MV+GNIT A+KEVY++GGRKFG++ VPPLGYMPSSRLKKSAQFFEEASSIAR+HNK L I
Sbjct: 204 MVIGNITKALKEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQFFEEASSIARLHNKLLPI 263

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 248
           AL KLSKQLKGFKY FAD HT LLQRI NP+ YGFKVVDTACCGSDEFRG+YNCGR+ GS
Sbjct: 264 ALHKLSKQLKGFKYAFADTHTLLLQRILNPSRYGFKVVDTACCGSDEFRGVYNCGRKLGS 323

Query: 249 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLAS 306
            P+THC+NLEDHMFFDSFHPT+KVFKQL ++ WSG  ++VKP NFKQLF Y+ +TLAS
Sbjct: 324 LPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQMWSGGLEVVKPYNFKQLFDYEPTTLAS 381

BLAST of Cucsat.G16029 vs. NCBI nr
Match: XP_023512752.1 (GDSL esterase/lipase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497 bits (1280), Expect = 2.59e-174
Identity = 234/298 (78.52%), Postives = 268/298 (89.93%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPL+PPYLDPHNDLYDYG NFASGGGGA+A+SHQEQA+GLQTQ
Sbjct: 84  RFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVNFASGGGGALALSHQEQALGLQTQ 143

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           M+FF++V+KSL+ KLG+AR++SF SNSVFLFNFGGNDYLNPFDISYDIFKTI+AQEQ+VN
Sbjct: 144 MKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVN 203

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MV+GNIT+A+KEVY++GGRKFG++ VPPLGYMPSSRLKKSAQFFEEASSIAR+HNK L I
Sbjct: 204 MVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQFFEEASSIARLHNKLLPI 263

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 248
           AL KLSKQLK FKY FAD HT LLQRI NP +YGFKVVDTACCGSDEFRG+YNCGR+ GS
Sbjct: 264 ALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLGS 323

Query: 249 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLAS 306
            P+THC+NLEDHMFFDSFHPT+KVFKQLA++ WSG  ++VKP NFKQLF Y+  TLAS
Sbjct: 324 LPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQLFEYEP-TLAS 380

BLAST of Cucsat.G16029 vs. ExPASy TrEMBL
Match: A0A0A0LQ38 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G296110 PE=3 SV=1)

HSP 1 Score: 592 bits (1525), Expect = 5.61e-212
Identity = 289/299 (96.66%), Postives = 291/299 (97.32%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ
Sbjct: 80  RFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 139

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           MEFFRKVEKSL+NKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN
Sbjct: 140 MEFFRKVEKSLRNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 199

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI
Sbjct: 200 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 259

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 248
           ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS
Sbjct: 260 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 319

Query: 249 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY 307
           SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY
Sbjct: 320 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY 378

BLAST of Cucsat.G16029 vs. ExPASy TrEMBL
Match: A0A5D3CQ75 (GDSL esterase/lipase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002190 PE=3 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 5.99e-198
Identity = 271/300 (90.33%), Postives = 284/300 (94.67%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPLIPPYLDPHNDLYDYG NFASGGGGA+A+SHQEQAIGLQTQ
Sbjct: 80  RFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYGVNFASGGGGALALSHQEQAIGLQTQ 139

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           M FFRKV KSL+NKLGHARS+SFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQ+VN
Sbjct: 140 MVFFRKVVKSLRNKLGHARSRSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQYVN 199

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MVVGNITIAIKEVY+YGGRKFGVLAVPPLGYMPSSRLKKSAQFFEE+SSIARIHNKF+LI
Sbjct: 200 MVVGNITIAIKEVYKYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEESSSIARIHNKFILI 259

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGR-EFG 248
           ALEKLSKQLKGFKYTFADVHTALLQRIQNPT+YGFKVVDTACCGSDEFRG+YNCGR +FG
Sbjct: 260 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTKYGFKVVDTACCGSDEFRGVYNCGRKDFG 319

Query: 249 SSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY 307
           S PYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGD DIVKP+NFKQLF  DDSTLASY
Sbjct: 320 SLPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDVDIVKPLNFKQLFSCDDSTLASY 379

BLAST of Cucsat.G16029 vs. ExPASy TrEMBL
Match: A0A1S4E2F5 (GDSL esterase/lipase 1-like OS=Cucumis melo OX=3656 GN=LOC103497528 PE=3 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 5.99e-198
Identity = 271/300 (90.33%), Postives = 284/300 (94.67%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPLIPPYLDPHNDLYDYG NFASGGGGA+A+SHQEQAIGLQTQ
Sbjct: 80  RFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYGVNFASGGGGALALSHQEQAIGLQTQ 139

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           M FFRKV KSL+NKLGHARS+SFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQ+VN
Sbjct: 140 MVFFRKVVKSLRNKLGHARSRSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQYVN 199

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MVVGNITIAIKEVY+YGGRKFGVLAVPPLGYMPSSRLKKSAQFFEE+SSIARIHNKF+LI
Sbjct: 200 MVVGNITIAIKEVYKYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEESSSIARIHNKFILI 259

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGR-EFG 248
           ALEKLSKQLKGFKYTFADVHTALLQRIQNPT+YGFKVVDTACCGSDEFRG+YNCGR +FG
Sbjct: 260 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTKYGFKVVDTACCGSDEFRGVYNCGRKDFG 319

Query: 249 SSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLASY 307
           S PYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGD DIVKP+NFKQLF  DDSTLASY
Sbjct: 320 SLPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDVDIVKPLNFKQLFSCDDSTLASY 379

BLAST of Cucsat.G16029 vs. ExPASy TrEMBL
Match: A0A6J1EIS0 (GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 6.78e-177
Identity = 235/298 (78.86%), Postives = 268/298 (89.93%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPL+PPYLDPHNDLYDYG NFASGGGGA+A+SHQEQA+GLQTQ
Sbjct: 84  RFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVNFASGGGGALALSHQEQALGLQTQ 143

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           M+FF++V+KSL+ KLG+AR++SF SNSVFLFNFGGNDYLNPFDISYDIFKTI+AQEQFVN
Sbjct: 144 MKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQFVN 203

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MV+GNIT A+KEVY++GGRKFG++ VPPLGYMPSSRLKKSAQFFEEASSIAR+HNK L I
Sbjct: 204 MVIGNITKALKEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQFFEEASSIARLHNKLLPI 263

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 248
           AL KLSKQLKGFKY FAD HT LLQRI NP+ YGFKVVDTACCGSDEFRG+YNCGR+ GS
Sbjct: 264 ALHKLSKQLKGFKYAFADTHTLLLQRILNPSRYGFKVVDTACCGSDEFRGVYNCGRKLGS 323

Query: 249 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLAS 306
            P+THC+NLEDHMFFDSFHPT+KVFKQL ++ WSG  ++VKP NFKQLF Y+ +TLAS
Sbjct: 324 LPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQMWSGGLEVVKPYNFKQLFDYEPTTLAS 381

BLAST of Cucsat.G16029 vs. ExPASy TrEMBL
Match: A0A6J1I9A0 (GDSL esterase/lipase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470360 PE=3 SV=1)

HSP 1 Score: 490 bits (1261), Expect = 9.74e-172
Identity = 229/298 (76.85%), Postives = 264/298 (88.59%), Query Frame = 0

Query: 9   RLRHGREVLKSNGEYANLPLIPPYLDPHNDLYDYGANFASGGGGAIAMSHQEQAIGLQTQ 68
           R   GR +    GEYANLPL+PPYLDPHNDLYDYG NFASGGGGA+ +SHQEQA+GLQTQ
Sbjct: 84  RFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVNFASGGGGALPLSHQEQALGLQTQ 143

Query: 69  MEFFRKVEKSLKNKLGHARSKSFLSNSVFLFNFGGNDYLNPFDISYDIFKTIEAQEQFVN 128
           M FF++V+KSL+ KLG+AR++SF SNSVFLFNFGGNDYLNPFDISYDIFKTI+ QEQFVN
Sbjct: 144 MNFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDTQEQFVN 203

Query: 129 MVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSSRLKKSAQFFEEASSIARIHNKFLLI 188
           MV+GNIT  +KEVY++GGRKFG++ VPPLGYMPSSRLKKSAQ+FEEASSIAR+HNK L I
Sbjct: 204 MVIGNITTVLKEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQYFEEASSIARLHNKLLPI 263

Query: 189 ALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGFKVVDTACCGSDEFRGIYNCGREFGS 248
           AL KLSKQLKGFKY FADVH  LLQRI NP++YGFKVVDTACCGSDEFRG+YNCGR+ GS
Sbjct: 264 ALHKLSKQLKGFKYAFADVHMLLLQRILNPSQYGFKVVDTACCGSDEFRGVYNCGRKLGS 323

Query: 249 SPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSGDEDIVKPVNFKQLFHYDDSTLAS 306
            P+THC+N+EDHMFFDS+HPT+KVFKQLA++ WSG  ++V P NFKQLF Y+  TLAS
Sbjct: 324 FPFTHCKNIEDHMFFDSYHPTEKVFKQLAEQMWSGGLEVVTPYNFKQLFEYEP-TLAS 380