Cucsat.G15601 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G15601
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	EKC/KEOPS complex, subunit Pcc1
Location	ctg2009: 2807405 .. 2809232 (+)
RNA-Seq Expression	Cucsat.G15601
Synteny	Cucsat.G15601

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTATAAAAAAAAAACAAAAAACAAATAAACCCTAAAATAATTCTCTCACATCCGAGTCAGTCGTCTTCGTCTTCAACGAGTCACGGTCTCAACTTCTTCCCATTTCTTCCACTGCCACGCCTCAGCCTCGCCGCCGACCATCCTCGCCACGCCTCCTACTACCCGCTGCCGTTACCGGTCAGTTTCAAAGCGCTCTTCATGCTCGTCATACCCTTTCATAAACTCAACCTTCTCTGTATTCTTATCTTTGCTTCATAGGTTTATTTGTGGGGGGAAATCTGGCTGTTTTTCATTAGAATTGAAGCAATGACTCCCACAGCTTCCGTCGCTTCTCATGGAGAGCCCAAATGGGAATTCAGCTGGTTGTTCCCTCTCTCTAAGTCCATAAATGTATAATTATAAGTTTTCCTTTTATTATATGCCATATGCTTTGAAATCGATTTCTTCGTAGATGTCCATGTTAACTTTTCCTCTTTAGCTTTATCTTTAATTAATTCTCCTGTGATTAATCATCTAAGTTCTTTCTCTGGTTCATTGCAATCTCCACAAACAAAATAAGACAATGATAATGATTATCATCTTGTTCTTGCTCTTGTCAACCCTTTGTCTGGTTTTCTTTTCACCCTGAAAGTTCAATTTTTTGTTGCTGAATCATACGGATTTTGTGGCCAAAGCAGTGTCATGAAAGCTTACTGTTTGAATGCCTTAAATTAGTTGTTGGTACTTATGTTATTTATAATTTTGACTCTAGGTTTAGATGAGCGTACTATATGTACAAACTCTCTGCTGTTAGAAGTTGTAATTCGTAATTTGATATTTCTCTCTCATAATAAAAATGTTCTCCTCCTTTGTCCGTTGATTTAAATAACACTATTAGTTAACCTTGTAAATCTCTCTGTCTATTTCTCTGTTGCTTACGTTTTCTGTTTTCTTTGATTGTCAATTGCCACCAAGTTCTCGGTTGTTGGAATGGAGTGGATAACTAGCCAAATCTCTCTTCTTTTGCTGTTGTTTTTGGAAGCCAAATTTGATTTTTCAGTCTTGTTAGCGTTCAGCAATTGCTACTTGTTTCTAAAACGGTCATCGCTTCTTTCCTTAAATAACCAACTGTTTTTTTATCATACATCGGAAACATGCAGTTCAACTCATTGTTAGCGATCTTTCATCTATTCTATATTGCCTTCATAAAGACATGGTTTGCCCTCTGCAGTGACTTTGAAGTCGATTATGAGTCTGAGAAGAAAGCTTCAATTGTCTATAAAGCTTTAGTCGTTGATAAAGAGGTGCTTCTCGATGATATCAATCTTCACCTTGTTCCTCCAGAGTTAACTTGTTTCTGATTCGATTTGTGTGCTATGAACAGTTACAGCCAGACAAGGTGAAAAGGGCGATGTCAAATTCTGATGGAAAACTTTCAATGTGAGCCTCTTTTTTGGTCCTTGCATTCATAGTTTTTAATGTACTTAGAGCTTAGTAATGCTATTGCATTCTATCATATTTTGTAGACATTTTGAGGCAGTGGAAGCAAGGTTTTTGCGTGCATCATTCAGTGCGTTTGTAGATGTGCTTACTCTTGCCACAAAAACAATCGAAGATTTTGGACAAGAAGAGGATTTCTGATAGCCTTACTGACCTATTTACATGGCTCTCAAATATCAAGTGTCATTTTTTTTTTTTTTTTTTGCTTAAAGTAGCTCTGGTTTTAATTTACATCTGACCTGTAATTTTATCACAATGTTTTGTCATTGTTTGATCGAGTGTGAAGGTCTTTCTCCACCCATCATTCGAACATTGAGATCTAACAAGATAAACATTTTCAGTAAACAATTTGTCCTTTTACTCCTTGCAGTCAACCATATAACTACTTGATATGTTATTTTTATGCCAAATGTAGTTTCAAGCATTTACTGATATGGTTAAATATACAAGGAATT

Coding sequence (CDS)

ATGACTCCCACAGCTTCCGTCGCTTCTCATGGAGAGCCCAAATGGGAATTCAGCTGTGACTTTGAAGTCGATTATGAGTCTGAGAAGAAAGCTTCAATTGTCTATAAAGCTTTAGTCGTTGATAAAGAGTTACAGCCAGACAAGGTGAAAAGGGCGATGTCAAATTCTGATGGAAAACTTTCAATACATTTTGAGGCAGTGGAAGCAAGGTTTTTGCGTGCATCATTCAGTGCGTTTGTAGATGTGCTTACTCTTGCCACAAAAACAATCGAAGATTTTGGACAAGAAGAGGATTTCTGA

Protein sequence

MTPTASVASHGEPKWEFSCDFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAFVDVLTLATKTIEDFGQEEDF

Homology

[Close]

BLAST of Cucsat.G15601 vs. NCBI nr
Match: XP_004146228.1 (uncharacterized protein LOC101217139 [Cucumis sativus] >KAE8650547.1 hypothetical protein Csa_010241 [Cucumis sativus])

HSP 1 Score: 154 bits (389), Expect = 4.96e-46
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 0


Query: 44  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 103
           DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF
Sbjct: 20  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 79

Query: 104 VDVLTLATKTIEDFGQEEDF 123
           VDVLTLATKTIEDFGQEEDF
Sbjct: 80  VDVLTLATKTIEDFGQEEDF 99

[Close]

BLAST of Cucsat.G15601 vs. NCBI nr
Match: XP_038895564.1 (uncharacterized protein LOC120083771 [Benincasa hispida])

HSP 1 Score: 144 bits (362), Expect = 6.42e-42
Identity = 74/80 (92.50%), Postives = 76/80 (95.00%), Query Frame = 0


Query: 44  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 103
           DFEVDYESEKKASIVY AL+VDKELQPDKVKR MS SDGKLSIHFEAVEARFLRASFSAF
Sbjct: 20  DFEVDYESEKKASIVYNALIVDKELQPDKVKRVMSVSDGKLSIHFEAVEARFLRASFSAF 79

Query: 104 VDVLTLATKTIEDFGQEEDF 123
           VDVLTLATKT+EDFGQE DF
Sbjct: 80  VDVLTLATKTVEDFGQEVDF 99

[Close]

BLAST of Cucsat.G15601 vs. NCBI nr
Match: XP_023553191.1 (uncharacterized protein LOC111810678 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142 bits (358), Expect = 2.61e-41
Identity = 75/80 (93.75%), Postives = 76/80 (95.00%), Query Frame = 0


Query: 44  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 103
           DFEVDYESEKKASIVYKALVVDKELQPDKVKR M+ SDGKLSIHFEAVEARFLRASFSAF
Sbjct: 20  DFEVDYESEKKASIVYKALVVDKELQPDKVKREMTVSDGKLSIHFEAVEARFLRASFSAF 79

Query: 104 VDVLTLATKTIEDFGQEEDF 123
           VDVLTLATKTIEDFGQ  DF
Sbjct: 80  VDVLTLATKTIEDFGQGVDF 99

[Close]

BLAST of Cucsat.G15601 vs. NCBI nr
Match: XP_022923038.1 (uncharacterized protein LOC111430837 [Cucurbita moschata])

HSP 1 Score: 139 bits (349), Expect = 4.96e-40
Identity = 75/88 (85.23%), Postives = 78/88 (88.64%), Query Frame = 0


Query: 36  PTTRCRYRDFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARF 95
           P + C   DFEVDYESEKKASIVYKALVVDKELQPDKVKR M+ SDGKLSIH EAVEARF
Sbjct: 8   PNSLC---DFEVDYESEKKASIVYKALVVDKELQPDKVKREMTVSDGKLSIHLEAVEARF 67

Query: 96  LRASFSAFVDVLTLATKTIEDFGQEEDF 123
           LRASFSAFVDVLTLATKTIEDFG+  DF
Sbjct: 68  LRASFSAFVDVLTLATKTIEDFGRGVDF 92

[Close]

BLAST of Cucsat.G15601 vs. NCBI nr
Match: XP_008466840.1 (PREDICTED: uncharacterized protein LOC103504146 [Cucumis melo] >XP_008466841.1 PREDICTED: uncharacterized protein LOC103504146 [Cucumis melo] >KAA0050520.1 EKC/KEOPS complex, subunit Pcc1 [Cucumis melo var. makuwa] >TYK11292.1 EKC/KEOPS complex, subunit Pcc1 [Cucumis melo var. makuwa])

HSP 1 Score: 138 bits (348), Expect = 8.70e-40
Identity = 72/80 (90.00%), Postives = 76/80 (95.00%), Query Frame = 0


Query: 44  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 103
           DFEVDYESEKKASIVY AL+VDKELQPDKVKRAMS S+GKLSIHFEAVEARFLRASFS+F
Sbjct: 20  DFEVDYESEKKASIVYNALIVDKELQPDKVKRAMSISNGKLSIHFEAVEARFLRASFSSF 79

Query: 104 VDVLTLATKTIEDFGQEEDF 123
           VDVLTLATKTIEDFG E +F
Sbjct: 80  VDVLTLATKTIEDFGLEVEF 99

[Close]

BLAST of Cucsat.G15601 vs. ExPASy TrEMBL
Match: A0A0A0L7A2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238730 PE=3 SV=1)

HSP 1 Score: 154 bits (389), Expect = 1.45e-45
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 0


Query: 44  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 103
           DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF
Sbjct: 78  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 137

Query: 104 VDVLTLATKTIEDFGQEEDF 123
           VDVLTLATKTIEDFGQEEDF
Sbjct: 138 VDVLTLATKTIEDFGQEEDF 157

[Close]

BLAST of Cucsat.G15601 vs. ExPASy TrEMBL
Match: A0A6J1E8H0 (uncharacterized protein LOC111430837 OS=Cucurbita moschata OX=3662 GN=LOC111430837 PE=3 SV=1)

HSP 1 Score: 139 bits (349), Expect = 2.40e-40
Identity = 75/88 (85.23%), Postives = 78/88 (88.64%), Query Frame = 0


Query: 36  PTTRCRYRDFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARF 95
           P + C   DFEVDYESEKKASIVYKALVVDKELQPDKVKR M+ SDGKLSIH EAVEARF
Sbjct: 8   PNSLC---DFEVDYESEKKASIVYKALVVDKELQPDKVKREMTVSDGKLSIHLEAVEARF 67

Query: 96  LRASFSAFVDVLTLATKTIEDFGQEEDF 123
           LRASFSAFVDVLTLATKTIEDFG+  DF
Sbjct: 68  LRASFSAFVDVLTLATKTIEDFGRGVDF 92

[Close]

BLAST of Cucsat.G15601 vs. ExPASy TrEMBL
Match: A0A1S3CTG0 (uncharacterized protein LOC103504146 OS=Cucumis melo OX=3656 GN=LOC103504146 PE=3 SV=1)

HSP 1 Score: 138 bits (348), Expect = 4.21e-40
Identity = 72/80 (90.00%), Postives = 76/80 (95.00%), Query Frame = 0


Query: 44  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 103
           DFEVDYESEKKASIVY AL+VDKELQPDKVKRAMS S+GKLSIHFEAVEARFLRASFS+F
Sbjct: 20  DFEVDYESEKKASIVYNALIVDKELQPDKVKRAMSISNGKLSIHFEAVEARFLRASFSSF 79

Query: 104 VDVLTLATKTIEDFGQEEDF 123
           VDVLTLATKTIEDFG E +F
Sbjct: 80  VDVLTLATKTIEDFGLEVEF 99

[Close]

BLAST of Cucsat.G15601 vs. ExPASy TrEMBL
Match: A0A5D3CJ23 (EKC/KEOPS complex, subunit Pcc1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001520 PE=3 SV=1)

HSP 1 Score: 138 bits (348), Expect = 4.21e-40
Identity = 72/80 (90.00%), Postives = 76/80 (95.00%), Query Frame = 0


Query: 44  DFEVDYESEKKASIVYKALVVDKELQPDKVKRAMSNSDGKLSIHFEAVEARFLRASFSAF 103
           DFEVDYESEKKASIVY AL+VDKELQPDKVKRAMS S+GKLSIHFEAVEARFLRASFS+F
Sbjct: 20  DFEVDYESEKKASIVYNALIVDKELQPDKVKRAMSISNGKLSIHFEAVEARFLRASFSSF 79

Query: 104 VDVLTLATKTIEDFGQEEDF 123
           VDVLTLATKTIEDFG E +F
Sbjct: 80  VDVLTLATKTIEDFGLEVEF 99

[Close]

BLAST of Cucsat.G15601 vs. ExPASy TrEMBL
Match: A0A6J1CHJ5 (uncharacterized protein LOC111011564 OS=Momordica charantia OX=3673 GN=LOC111011564 PE=3 SV=1)

HSP 1 Score: 137 bits (345), Expect = 2.42e-39
Identity = 79/111 (71.17%), Postives = 82/111 (73.87%), Query Frame = 0


Query: 24  ASASPPTILATPPTT------------RCRYRDFEVDYESEKKASIVYKALVVDKELQPD 83
           +  S PT  A  PT             RC   DFEVDYESEKKASIVY AL VDKELQPD
Sbjct: 14  SGVSSPTTEAMAPTASVASDGEAKWEFRC---DFEVDYESEKKASIVYNALAVDKELQPD 73

Query: 84  KVKRAMSNSDGKLSIHFEAVEARFLRASFSAFVDVLTLATKTIEDFGQEED 122
           KVKR M+ SDGKLS+HFEAVEARFLRASFSAFVDVLTLATKTIEDFGQ  D
Sbjct: 74  KVKREMAVSDGKLSVHFEAVEARFLRASFSAFVDVLTLATKTIEDFGQGLD 121

The following BLAST results are available for this feature:

BLAST of Cucsat.G15601 vs.


Match Name	E-value	Identity	Description

BLAST of Cucsat.G15601 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146228.1	4.96e-46	100.00	uncharacterized protein LOC101217139 [Cucumis sativus] >KAE8650547.1 hypothetica...	[more]
XP_038895564.1	6.42e-42	92.50	uncharacterized protein LOC120083771 [Benincasa hispida]	[more]
XP_023553191.1	2.61e-41	93.75	uncharacterized protein LOC111810678 [Cucurbita pepo subsp. pepo]	[more]
XP_022923038.1	4.96e-40	85.23	uncharacterized protein LOC111430837 [Cucurbita moschata]	[more]
XP_008466840.1	8.70e-40	90.00	PREDICTED: uncharacterized protein LOC103504146 [Cucumis melo] >XP_008466841.1 P...	[more]

BLAST of Cucsat.G15601 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7A2	1.45e-45	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238730 PE=3 SV=1	[more]
A0A6J1E8H0	2.40e-40	85.23	uncharacterized protein LOC111430837 OS=Cucurbita moschata OX=3662 GN=LOC1114308...	[more]
A0A1S3CTG0	4.21e-40	90.00	uncharacterized protein LOC103504146 OS=Cucumis melo OX=3656 GN=LOC103504146 PE=...	[more]
A0A5D3CJ23	4.21e-40	90.00	EKC/KEOPS complex, subunit Pcc1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1CHJ5	2.42e-39	71.17	uncharacterized protein LOC111011564 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015419	CTAG/Pcc1 family	PFAM	PF09341	Pcc1	coord: 28..98 e-value: 1.2E-17 score: 63.8
IPR015419	CTAG/Pcc1 family	PANTHER	PTHR31283	EKC/KEOPS COMPLEX SUBUNIT PCC1 FAMILY MEMBER	coord: 25..103
None	No IPR available	GENE3D	3.30.310.50		coord: 21..105 e-value: 1.7E-20 score: 74.8
None	No IPR available	PANTHER	PTHR31283:SF5	GEO08993P1	coord: 25..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G15601.T1	Cucsat.G15601.T1	mRNA
Cucsat.G15601.T4	Cucsat.G15601.T4	mRNA
Cucsat.G15601.T5	Cucsat.G15601.T5	mRNA
Cucsat.G15601.T2	Cucsat.G15601.T2	mRNA
Cucsat.G15601.T19	Cucsat.G15601.T19	mRNA
Cucsat.G15601.T18	Cucsat.G15601.T18	mRNA
Cucsat.G15601.T15	Cucsat.G15601.T15	mRNA
Cucsat.G15601.T6	Cucsat.G15601.T6	mRNA
Cucsat.G15601.T24	Cucsat.G15601.T24	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070525	tRNA threonylcarbamoyladenosine metabolic process
cellular_component	GO:0000408	EKC/KEOPS complex