Cucsat.G1549 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G1549
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	GDSL esterase/lipase
Location	ctg1000: 1377992 .. 1379420 (-)
RNA-Seq Expression	Cucsat.G1549
Synteny	Cucsat.G1549

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTACGGTAACCTCCGCCACCGCCACCGGAGCCACCCTTCACGGGGAACAAAACTATTTGTTTTTGGTGATTCATATGTTGATACCGGCAACATTCTGTTTCCGTTTTCAAGTGCTGAACAGTTTCCTTATGGAATCACTTTCCCCGGAAAACCCAGTGGTCGGTTTTCGGATGGTCGTGTTCTGACTGACTTTGCAGCGAAACATTTAGGGGTGAAATCTCCGATACCGTTTAGTATACGAAGTGAAGTTGGGGAGGAGAGATTGAAAGAATCAGGGATAAATTTTGCATTTGGTGGAACAGGAGTTTTCAACACGTTGGTTCCTCTCCCAAATATGACAACACAAATTGACCTGTTTGAACAACTTCGAGATGACGAATCTGGGTTGATCAGTAATAGAGATGTTCATCTCTCTCTCGCACTTGTCTCTGTTTCTGGGAATGATTACTCTTTTTACCTTGCAACTAATGGCTCTGCCCAGGTAATTCCTTCATCATCTCCATAACTTTCTCTACTTTAACATTGTTTATCACTATTATTCATTTTTAATCATCACTTGCTTTCTCCTCTCTCACAATACAGTACTGATTATGTTTGTTTATCTTAATAGAATCTCTTAATTTTACGCTGAAAAATAGAATAGAGAGTGGACAGAAATAGATAATAAAATGGACAATCCACCCATTCTAAATAATCTATACCCATATAGAGAATTTAAATGGGGGGCGTGGACAAGGACGACTATTTGTTGTCACAATTTATTAGATAATACAAAATTTTATGACACAGCCAAATTGACGAGTCACTGACTAGGCGGAGATTTTTTACTTTATGAAATTTTTAATATATCTTGAAGATGATATCTTTTTGGTTCACTGTTTTTAGTTTCTATCCAAATATTTATTTCAATTTTTTTTTTATGACGTCGTGGGTCTTAATACCATATTATATAGTCTATCGATAGATTATTACAAGGCTATGAAAGTTAATTTGTTTGATTGTACAGGGATTAAAACCATTCATAAACTCGGTGGTGAATCAGATAATGGTAGATTTGAGGAGGATTAGGAGGTTAGGTGTAAAGAAAATAGTAGTGACAGGGCTAGGGCCATTGGGATGTCTTCCAATATTTACAGCTCCATTCTCATTCAAGCAATGCAATCAAACCATAAACTCATTTGTCCAATTTCACAACTTCTTGTTGAAGCAAGCTGTGGATAAACTCAACAAACAAATTACTAAACACCAGCATTATTCTTCTTCTTCTTCTTCAAAAATCTTCATTCTTGATGTGTATGATGCATTCTTGTCCATAATCCAAGGCCGTGGCAGTGGCAGTGGCAGTGGCAGAGTTGGGTTATTAAAGTTCAAGACCCCATTAAAGCCATGTTGTTTTGGGGTGAGCAGTGAGTTTGAATGTGGGAGTGTG

Coding sequence (CDS)

TACGGTAACCTCCGCCACCGCCACCGGAGCCACCCTTCACGGGGAACAAAACTATTTGTTTTTGGTGATTCATATGTTGATACCGGCAACATTCTGTTTCCGTTTTCAAGTGCTGAACAGTTTCCTTATGGAATCACTTTCCCCGGAAAACCCAGTGGTCGGTTTTCGGATGGTCGTGTTCTGACTGACTTTGCAGCGAAACATTTAGGGGTGAAATCTCCGATACCGTTTAGTATACGAAGTGAAGTTGGGGAGGAGAGATTGAAAGAATCAGGGATAAATTTTGCATTTGGTGGAACAGGAGTTTTCAACACGTTGGTTCCTCTCCCAAATATGACAACACAAATTGACCTGTTTGAACAACTTCGAGATGACGAATCTGGGTTGATCAGTAATAGAGATGTTCATCTCTCTCTCGCACTTGTCTCTGTTTCTGGGAATGATTACTCTTTTTACCTTGCAACTAATGGCTCTGCCCAGGGATTAAAACCATTCATAAACTCGGTGGTGAATCAGATAATGGTAGATTTGAGGAGGATTAGGAGGTTAGGTGTAAAGAAAATAGTAGTGACAGGGCTAGGGCCATTGGGATGTCTTCCAATATTTACAGCTCCATTCTCATTCAAGCAATGCAATCAAACCATAAACTCATTTGTCCAATTTCACAACTTCTTGTTGAAGCAAGCTGTGGATAAACTCAACAAACAAATTACTAAACACCAGCATTATTCTTCTTCTTCTTCTTCAAAAATCTTCATTCTTGATGTGTATGATGCATTCTTGTCCATAATCCAAGGCCGTGGCAGTGGCAGTGGCAGTGGCAGAGTTGGGTTATTAAAGTTCAAGACCCCATTAAAGCCATGTTGTTTTGGGGTGAGCAGTGAGTTTGAATGTGGGAGTGTG

Protein sequence

YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSV

Homology

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BLAST of Cucsat.G1549 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 8.1e-83
Identity = 161/294 (54.76%), Postives = 202/294 (68.71%), Query Frame = 0


Query: 8   HRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAK 67
           H  +P R TKLFVFGDSY DTGNI   FSS+ +FPYGITFPGKP+GRFSDGRV TDF AK
Sbjct: 34  HHLYPFRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAK 93

Query: 68  HLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDES 127
            +G+KSPIP+  +   G++RL + G+NFA+GGTGVFNT  PLPNMTTQID+F+ +     
Sbjct: 94  FVGIKSPIPYFWKDYAGKKRL-QYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNIL-TTG 153

Query: 128 GLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKK 187
            +    ++  S+ALVSV+GNDYS ++A N  A     FI  VV+Q  V+LRRI  LGVKK
Sbjct: 154 DIYYPPELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKK 213

Query: 188 IVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSS 247
           I V  L PLGCLP FT   SF++CN+T N+ V  HN LL+Q V KLN +         + 
Sbjct: 214 IAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNE---------TK 273

Query: 248 SSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSV 302
            S   ILD+Y+AFL++ + +GS  GS R     F++PLKPCC GVS E+ CGSV
Sbjct: 274 QSTFIILDLYNAFLTVFKNKGSNPGSTR-----FESPLKPCCVGVSREYNCGSV 311

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BLAST of Cucsat.G1549 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.9e-70
Identity = 141/289 (48.79%), Postives = 187/289 (64.71%), Query Frame = 0


Query: 14  RGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKS 73
           R  +LFVFGDSY DTGNI    S + + PYGITFP KPSGRFSDGRV TDF A++LG+KS
Sbjct: 36  RPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKS 95

Query: 74  PIPFSIRSEVGEERLKESGINFAFGGTGVFNTL-VPLPNMTTQIDLFEQLRDDESGLISN 133
           PIP++ +   G+ERL   G+N+A+GGTGVF T   PLPNMTTQID F+++      + S 
Sbjct: 96  PIPYTWKDYAGKERLL-YGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVL-AAGNIYSP 155

Query: 134 RDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTG 193
            D+  SLALVSV+GNDY+ +LA       L  F+  VV+QI V+  RI +LGV KIV+  
Sbjct: 156 SDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPS 215

Query: 194 LGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIF 253
           + PLGCLP  T   SF++CN T N+    HN+LL +A+ +LN +         +  S   
Sbjct: 216 MQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNE---------TKPSTFV 275

Query: 254 ILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSV 302
           +LD Y+AFL++ + +G   G  R G      PLKPCC GV+S ++C +V
Sbjct: 276 VLDHYNAFLTVFKNKGPEPGVSRFG-----NPLKPCCVGVNSSYDCSNV 308

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BLAST of Cucsat.G1549 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 8.2e-51
Identity = 113/285 (39.65%), Postives = 168/285 (58.95%), Query Frame = 0


Query: 12  PSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGV 71
           P +  KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  K LG 
Sbjct: 9   PRKVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGA 68

Query: 72  KSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL--VPLPNMTTQIDLFEQLRDDESGL 131
           +SP  +      G ++  + G+NFAFGG+ + ++    P PN+T Q++    L    +G 
Sbjct: 69  ESPYLYRTH---GRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL--VLAGR 128

Query: 132 ISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIV 191
           +        ++L+S +G DY +Y+  N  A GLK  +  VV+ + V++  +  L  KKI 
Sbjct: 129 VYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIA 188

Query: 192 VTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ---ITKHQHYSSS 251
           VT L P+GCLP +T+  SFK CN++ ++ V+ HN LLK+ V KLN+Q   + K QH+   
Sbjct: 189 VTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF--- 248

Query: 252 SSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFG 292
                FI+D+++AF+++++ +GS          +FK P+K CC G
Sbjct: 249 -----FIIDIHNAFMTVMKNKGS---------KRFKNPMKSCCEG 270

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BLAST of Cucsat.G1549 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 4.5e-49
Identity = 120/287 (41.81%), Postives = 166/287 (57.84%), Query Frame = 0


Query: 16  TKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPI 75
           TKLFVFGDSY DTGN  F    + +FP GITFPG P+GRFSDGRV TD+ AK++GV++PI
Sbjct: 46  TKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTPI 105

Query: 76  PFSIRSEVGEERLK-ESGINFAFGGTGVFNTLVPL-PNMTTQIDLFEQLRDDESGLISNR 135
            +    + G  RL  + G+NFA+GG G F T+  L P  + QID FEQL      + S  
Sbjct: 106 TYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLL--MRNVYSPA 165

Query: 136 DVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGL 195
           D++ S+A  S+ GNDY  Y   NGS +G       VV QI++D++RI+ LGV+K++V   
Sbjct: 166 DLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALS 225

Query: 196 GPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN-KQITKHQHYSSSSSSKIF 255
            P  CLP    P   K C+ T ++    HN LL++ + KLN K+I       +++     
Sbjct: 226 PPQKCLPKLVTP---KGCD-TNDTSTYLHNSLLRKGLIKLNDKEI-------NNNDKSFM 285

Query: 256 ILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECG 300
            LD+Y+AF++I + +G        G+  F  P K CC      F CG
Sbjct: 286 TLDLYNAFVTIFKNKGVS------GVSTFPDPFKACCATKRGTF-CG 311

[Close]

BLAST of Cucsat.G1549 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 194.5 bits (493), Expect = 1.7e-48
Identity = 114/284 (40.14%), Postives = 165/284 (58.10%), Query Frame = 0


Query: 10  SHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHL 69
           S  ++  KLFVFG+SY DTGN + P + + + PYGITFPGKPSGR+SDG   TDF AK L
Sbjct: 27  SQANKNPKLFVFGNSYADTGN-MKPTALSWKLPYGITFPGKPSGRYSDGLTATDFLAKQL 86

Query: 70  GVKSPIPFSIRSEVGEERLK-ESGINFAFGGTGVFNTLVP-LPNMTTQIDLFEQLRDDES 129
           G K  +P+  R+  G++++K   G+NFAFGG+ VF++ V   PN++TQ+     L     
Sbjct: 87  GAK--LPYLWRTH-GKKKVKLNRGMNFAFGGSEVFDSPVDRSPNISTQVGFLVNLALARR 146

Query: 130 GLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKK 189
               + D+  S AL+S SG DY  ++  N +      F+  +V  I   L  +  L  K 
Sbjct: 147 VYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKN 206

Query: 190 IVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSS 249
           I VT L PLGCLP  T   SF+ CN++ +  V+ HN  LK+AV KLNK     +    + 
Sbjct: 207 IAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLHNESLKKAVAKLNK-----EDKFRTK 266

Query: 250 SSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFG 292
             +  I+D++ AF++I++ +G+          +FK+PLKPCC G
Sbjct: 267 GDRFVIVDLHKAFMTILEKKGN---------KRFKSPLKPCCEG 292

[Close]

BLAST of Cucsat.G1549 vs. NCBI nr
Match: XP_004143379.2 (GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protein Csa_003217 [Cucumis sativus])

HSP 1 Score: 585 bits (1507), Expect = 3.35e-209
Identity = 296/302 (98.01%), Postives = 296/302 (98.01%), Query Frame = 0


Query: 1   YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV 60
           YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV
Sbjct: 29  YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV 88

Query: 61  LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE 120
           LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE
Sbjct: 89  LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE 148

Query: 121 QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI 180
           QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI
Sbjct: 149 QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI 208

Query: 181 RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKH 240
           RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITK 
Sbjct: 209 RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKQ 268

Query: 241 QHYSSSSSS-KIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECG 300
           QHYSSSSSS KIFILDVYDAFLSIIQGRGSG    RVGLLKFKTPLKPCCFGVSSEFECG
Sbjct: 269 QHYSSSSSSSKIFILDVYDAFLSIIQGRGSG----RVGLLKFKTPLKPCCFGVSSEFECG 326

[Close]

BLAST of Cucsat.G1549 vs. NCBI nr
Match: XP_008462669.1 (PREDICTED: GDSL esterase/lipase At5g03610-like, partial [Cucumis melo])

HSP 1 Score: 522 bits (1344), Expect = 1.30e-184
Identity = 270/308 (87.66%), Postives = 280/308 (90.91%), Query Frame = 0


Query: 1   YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV 60
           Y + RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSGRFSDGRV
Sbjct: 10  YYSNRH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRV 69

Query: 61  LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE 120
           LTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPNMTTQIDLFE
Sbjct: 70  LTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQIDLFE 129

Query: 121 QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI 180
           QLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ+M+DL+RI
Sbjct: 130 QLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRI 189

Query: 181 RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKH 240
           +RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQIT  
Sbjct: 190 KRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQ 249

Query: 241 QHY-------SSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVS 300
           QH        SSSSSSKIFILDVYDAFLS+IQGRGSG    RVGLLKFKTPLKPCCFGVS
Sbjct: 250 QHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSG----RVGLLKFKTPLKPCCFGVS 309

[Close]

BLAST of Cucsat.G1549 vs. NCBI nr
Match: TYK07397.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 522 bits (1344), Expect = 1.35e-184
Identity = 270/308 (87.66%), Postives = 280/308 (90.91%), Query Frame = 0


Query: 1   YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV 60
           Y + RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSGRFSDGRV
Sbjct: 11  YYSNRH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRV 70

Query: 61  LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE 120
           LTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPNMTTQIDLFE
Sbjct: 71  LTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQIDLFE 130

Query: 121 QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI 180
           QLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ+M+DL+RI
Sbjct: 131 QLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRI 190

Query: 181 RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKH 240
           +RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQIT  
Sbjct: 191 KRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQ 250

Query: 241 QHY-------SSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVS 300
           QH        SSSSSSKIFILDVYDAFLS+IQGRGSG    RVGLLKFKTPLKPCCFGVS
Sbjct: 251 QHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSG----RVGLLKFKTPLKPCCFGVS 310

[Close]

BLAST of Cucsat.G1549 vs. NCBI nr
Match: KAA0025271.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 522 bits (1344), Expect = 2.50e-184
Identity = 270/308 (87.66%), Postives = 280/308 (90.91%), Query Frame = 0


Query: 1   YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV 60
           Y + RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSGRFSDGRV
Sbjct: 28  YYSNRH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRV 87

Query: 61  LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE 120
           LTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPNMTTQIDLFE
Sbjct: 88  LTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQIDLFE 147

Query: 121 QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI 180
           QLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ+M+DL+RI
Sbjct: 148 QLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRI 207

Query: 181 RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKH 240
           +RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQIT  
Sbjct: 208 KRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQ 267

Query: 241 QHY-------SSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVS 300
           QH        SSSSSSKIFILDVYDAFLS+IQGRGSG    RVGLLKFKTPLKPCCFGVS
Sbjct: 268 QHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSG----RVGLLKFKTPLKPCCFGVS 327

[Close]

BLAST of Cucsat.G1549 vs. NCBI nr
Match: XP_038883594.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 472 bits (1215), Expect = 8.34e-165
Identity = 247/302 (81.79%), Postives = 262/302 (86.75%), Query Frame = 0


Query: 1   YGNLRHRH-RSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGR 60
           YG+  H H R H  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSGRFSDGR
Sbjct: 31  YGDHHHHHHRRHRLRPTKLFVFGDSYVDTGNILLPFSSAQQFPYGITFPGKPSGRFSDGR 90

Query: 61  VLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLF 120
           VLTDF AKHLGVKSPIPFSIRSEVG ERLKESGINFAFGGTGVF+T VPLPNMTTQID F
Sbjct: 91  VLTDFLAKHLGVKSPIPFSIRSEVGVERLKESGINFAFGGTGVFDTSVPLPNMTTQIDFF 150

Query: 121 EQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRR 180
           + LR  ES + SN D   S+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQI++DL+R
Sbjct: 151 QHLRHVES-VFSNGDAQHSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIVIDLKR 210

Query: 181 IRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITK 240
           IRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFV+FHNFLL QAV KLNK+ TK
Sbjct: 211 IRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVRFHNFLLNQAVAKLNKE-TK 270

Query: 241 HQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECG 300
           H    +SSSSKIF+LDVYDAFLSIIQ R    G     +L+FKTPLKPCCFGVSS FECG
Sbjct: 271 H----ASSSSKIFVLDVYDAFLSIIQSRSKKPGV----VLEFKTPLKPCCFGVSSGFECG 322

[Close]

BLAST of Cucsat.G1549 vs. ExPASy TrEMBL
Match: A0A1S3CJ29 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=3 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 6.31e-185
Identity = 270/308 (87.66%), Postives = 280/308 (90.91%), Query Frame = 0


Query: 1   YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV 60
           Y + RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSGRFSDGRV
Sbjct: 10  YYSNRH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRV 69

Query: 61  LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE 120
           LTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPNMTTQIDLFE
Sbjct: 70  LTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQIDLFE 129

Query: 121 QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI 180
           QLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ+M+DL+RI
Sbjct: 130 QLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRI 189

Query: 181 RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKH 240
           +RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQIT  
Sbjct: 190 KRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQ 249

Query: 241 QHY-------SSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVS 300
           QH        SSSSSSKIFILDVYDAFLS+IQGRGSG    RVGLLKFKTPLKPCCFGVS
Sbjct: 250 QHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSG----RVGLLKFKTPLKPCCFGVS 309

[Close]

BLAST of Cucsat.G1549 vs. ExPASy TrEMBL
Match: A0A5D3C686 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001210 PE=3 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 6.54e-185
Identity = 270/308 (87.66%), Postives = 280/308 (90.91%), Query Frame = 0


Query: 1   YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV 60
           Y + RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSGRFSDGRV
Sbjct: 11  YYSNRH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRV 70

Query: 61  LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE 120
           LTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPNMTTQIDLFE
Sbjct: 71  LTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQIDLFE 130

Query: 121 QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI 180
           QLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ+M+DL+RI
Sbjct: 131 QLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRI 190

Query: 181 RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKH 240
           +RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQIT  
Sbjct: 191 KRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQ 250

Query: 241 QHY-------SSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVS 300
           QH        SSSSSSKIFILDVYDAFLS+IQGRGSG    RVGLLKFKTPLKPCCFGVS
Sbjct: 251 QHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSG----RVGLLKFKTPLKPCCFGVS 310

[Close]

BLAST of Cucsat.G1549 vs. ExPASy TrEMBL
Match: A0A5A7SLZ4 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001230 PE=3 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 1.21e-184
Identity = 270/308 (87.66%), Postives = 280/308 (90.91%), Query Frame = 0


Query: 1   YGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRV 60
           Y + RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFPGKPSGRFSDGRV
Sbjct: 28  YYSNRH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRV 87

Query: 61  LTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFE 120
           LTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VPLPNMTTQIDLFE
Sbjct: 88  LTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQIDLFE 147

Query: 121 QLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRI 180
           QLR  ESGL SNRDVHLS+ALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ+M+DL+RI
Sbjct: 148 QLRHAESGL-SNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRI 207

Query: 181 RRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKH 240
           +RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQIT  
Sbjct: 208 KRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQ 267

Query: 241 QHY-------SSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVS 300
           QH        SSSSSSKIFILDVYDAFLS+IQGRGSG    RVGLLKFKTPLKPCCFGVS
Sbjct: 268 QHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSG----RVGLLKFKTPLKPCCFGVS 327

[Close]

BLAST of Cucsat.G1549 vs. ExPASy TrEMBL
Match: A0A6J1EC94 (GDSL esterase/lipase At5g03610-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 401 bits (1031), Expect = 2.98e-137
Identity = 204/299 (68.23%), Postives = 237/299 (79.26%), Query Frame = 0


Query: 3   NLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLT 62
           N  H H+ H  R TKLFVFGDSYVDTGNIL P SSA+QFPYGITFPGKPSGRFSDGRVLT
Sbjct: 29  NHHHHHQRHRLRPTKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSGRFSDGRVLT 88

Query: 63  DFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFEQL 122
           DFAA+++G+KSPIPF++++ VG  R KE+G+NFAFGGTGVF+T  P PNMTTQID F+QL
Sbjct: 89  DFAARYVGLKSPIPFTVQNRVGLRRFKETGVNFAFGGTGVFDTSFPFPNMTTQIDFFQQL 148

Query: 123 RDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRR 182
           +  ES L +  D   S ALVSVSGNDYS  + TNG  QGLKPFIN+VVNQI+VDL+RI  
Sbjct: 149 QHGES-LSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIVDLKRIGE 208

Query: 183 LGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKHQH 242
           LGVKKI VTGLGPLGCLP FTAP SFK+CN+TINSFV+FHNFLLK+AV KLN++ TKH  
Sbjct: 209 LGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLNQE-TKH-- 268

Query: 243 YSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSV 301
                SSK+F+LD+Y AF+SII+G    +         FKTPLKPCCFGV S F+CGSV
Sbjct: 269 -----SSKVFVLDMYGAFMSIIEGEKRYNNG------VFKTPLKPCCFGVKSGFDCGSV 312

[Close]

BLAST of Cucsat.G1549 vs. ExPASy TrEMBL
Match: A0A6J1ECT1 (GDSL esterase/lipase At5g03610-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 400 bits (1027), Expect = 1.34e-136
Identity = 203/297 (68.35%), Postives = 235/297 (79.12%), Query Frame = 0


Query: 5   RHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDF 64
           R RH  H  R TKLFVFGDSY DTGNILFP S A QFPYGITFPGKP GRFSDGRVLTDF
Sbjct: 34  RRRHLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDF 93

Query: 65  AAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRD 124
           AA+H+G+KSPIPFS+R+ VG  R KE+G+NFAFGGTGVF+T  PLPNMTTQID F+QLR 
Sbjct: 94  AARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRH 153

Query: 125 DESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLG 184
            +S + +N D   S+ALVS SGNDY+FY A N S QGLKPFI SVV+QI+V+L+RI  LG
Sbjct: 154 GDS-IFTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLG 213

Query: 185 VKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYS 244
           VKKI VTGLGPLGCLP+FTAP S+KQCN+TINS VQFHN LLK+AV KLN++ TKH    
Sbjct: 214 VKKIAVTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLKEAVKKLNQE-TKH---- 273

Query: 245 SSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSV 301
              SSK+F++DVY AF+SII+GR   +        +FKTPLKPCC+GV S F CGSV
Sbjct: 274 ---SSKVFVVDVYGAFMSIIEGRKRYNNG------EFKTPLKPCCYGVGSGFSCGSV 315

[Close]

BLAST of Cucsat.G1549 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 308.5 bits (789), Expect = 5.8e-84
Identity = 161/294 (54.76%), Postives = 202/294 (68.71%), Query Frame = 0


Query: 8   HRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAK 67
           H  +P R TKLFVFGDSY DTGNI   FSS+ +FPYGITFPGKP+GRFSDGRV TDF AK
Sbjct: 34  HHLYPFRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAK 93

Query: 68  HLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDES 127
            +G+KSPIP+  +   G++RL + G+NFA+GGTGVFNT  PLPNMTTQID+F+ +     
Sbjct: 94  FVGIKSPIPYFWKDYAGKKRL-QYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNIL-TTG 153

Query: 128 GLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKK 187
            +    ++  S+ALVSV+GNDYS ++A N  A     FI  VV+Q  V+LRRI  LGVKK
Sbjct: 154 DIYYPPELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKK 213

Query: 188 IVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSS 247
           I V  L PLGCLP FT   SF++CN+T N+ V  HN LL+Q V KLN +         + 
Sbjct: 214 IAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNE---------TK 273

Query: 248 SSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSV 302
            S   ILD+Y+AFL++ + +GS  GS R     F++PLKPCC GVS E+ CGSV
Sbjct: 274 QSTFIILDLYNAFLTVFKNKGSNPGSTR-----FESPLKPCCVGVSREYNCGSV 311

[Close]

BLAST of Cucsat.G1549 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 5.6e-71
Identity = 141/289 (48.79%), Postives = 187/289 (64.71%), Query Frame = 0


Query: 14  RGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKS 73
           R  +LFVFGDSY DTGNI    S + + PYGITFP KPSGRFSDGRV TDF A++LG+KS
Sbjct: 36  RPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKS 95

Query: 74  PIPFSIRSEVGEERLKESGINFAFGGTGVFNTL-VPLPNMTTQIDLFEQLRDDESGLISN 133
           PIP++ +   G+ERL   G+N+A+GGTGVF T   PLPNMTTQID F+++      + S 
Sbjct: 96  PIPYTWKDYAGKERLL-YGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVL-AAGNIYSP 155

Query: 134 RDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTG 193
            D+  SLALVSV+GNDY+ +LA       L  F+  VV+QI V+  RI +LGV KIV+  
Sbjct: 156 SDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPS 215

Query: 194 LGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITKHQHYSSSSSSKIF 253
           + PLGCLP  T   SF++CN T N+    HN+LL +A+ +LN +         +  S   
Sbjct: 216 MQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNE---------TKPSTFV 275

Query: 254 ILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECGSV 302
           +LD Y+AFL++ + +G   G  R G      PLKPCC GV+S ++C +V
Sbjct: 276 VLDHYNAFLTVFKNKGPEPGVSRFG-----NPLKPCCVGVNSSYDCSNV 308

[Close]

BLAST of Cucsat.G1549 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 202.2 bits (513), Expect = 5.8e-52
Identity = 113/285 (39.65%), Postives = 168/285 (58.95%), Query Frame = 0


Query: 12  PSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGV 71
           P +  KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  K LG 
Sbjct: 9   PRKVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGA 68

Query: 72  KSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL--VPLPNMTTQIDLFEQLRDDESGL 131
           +SP  +      G ++  + G+NFAFGG+ + ++    P PN+T Q++    L    +G 
Sbjct: 69  ESPYLYRTH---GRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL--VLAGR 128

Query: 132 ISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIV 191
           +        ++L+S +G DY +Y+  N  A GLK  +  VV+ + V++  +  L  KKI 
Sbjct: 129 VYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIA 188

Query: 192 VTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ---ITKHQHYSSS 251
           VT L P+GCLP +T+  SFK CN++ ++ V+ HN LLK+ V KLN+Q   + K QH+   
Sbjct: 189 VTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF--- 248

Query: 252 SSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFG 292
                FI+D+++AF+++++ +GS          +FK P+K CC G
Sbjct: 249 -----FIIDIHNAFMTVMKNKGS---------KRFKNPMKSCCEG 270

[Close]

BLAST of Cucsat.G1549 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 196.4 bits (498), Expect = 3.2e-50
Identity = 120/287 (41.81%), Postives = 166/287 (57.84%), Query Frame = 0


Query: 16  TKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPI 75
           TKLFVFGDSY DTGN  F    + +FP GITFPG P+GRFSDGRV TD+ AK++GV++PI
Sbjct: 46  TKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTPI 105

Query: 76  PFSIRSEVGEERLK-ESGINFAFGGTGVFNTLVPL-PNMTTQIDLFEQLRDDESGLISNR 135
            +    + G  RL  + G+NFA+GG G F T+  L P  + QID FEQL      + S  
Sbjct: 106 TYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLL--MRNVYSPA 165

Query: 136 DVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGL 195
           D++ S+A  S+ GNDY  Y   NGS +G       VV QI++D++RI+ LGV+K++V   
Sbjct: 166 DLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALS 225

Query: 196 GPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN-KQITKHQHYSSSSSSKIF 255
            P  CLP    P   K C+ T ++    HN LL++ + KLN K+I       +++     
Sbjct: 226 PPQKCLPKLVTP---KGCD-TNDTSTYLHNSLLRKGLIKLNDKEI-------NNNDKSFM 285

Query: 256 ILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFECG 300
            LD+Y+AF++I + +G        G+  F  P K CC      F CG
Sbjct: 286 TLDLYNAFVTIFKNKGVS------GVSTFPDPFKACCATKRGTF-CG 311

[Close]

BLAST of Cucsat.G1549 vs. TAIR 10
Match: AT5G41890.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 119.8 bits (299), Expect = 3.8e-27
Identity = 86/300 (28.67%), Postives = 140/300 (46.67%), Query Frame = 0


Query: 19  FVFGDSYVDTG--NILFPFSSAEQFPYGITFP---GKPSGRFSDGRVLTDFAAKHLGVKS 78
           F+FGDS VD G  N +F  S A+  PYGI F    G+P+GRF++GR ++D   + LG KS
Sbjct: 33  FIFGDSLVDVGNNNYIFTLSKADSSPYGIDFAPSNGQPTGRFTNGRTISDIVGEALGAKS 92

Query: 79  PIPFSIRSEVGEERLKESGINFAFGGTGVFN----TLVPLPNMTTQIDLFEQLRDDESGL 138
           P P  +     E     +GIN+A G  G+ +      +    +  Q+  FE+ R+    +
Sbjct: 93  PPPPYLEPNT-EANTIRNGINYASGAAGILDDTGLLFIGRVPLREQVSNFEKSREYMVRV 152

Query: 139 ISNRD----VHLSLALVSVSGNDYSFYLATN--GSAQGLKP---FINSVVNQIMVDLRRI 198
           I        +  ++  +++  ND   Y+  +    +Q   P     +S+V  +   L+R+
Sbjct: 153 IGENGTKEMLKNAMFTITIGSNDILNYIQPSIPFFSQDKLPTDVLQDSMVLHLTTHLKRL 212

Query: 199 RRLGVKKIVVTGLGPLGCLPIFTA--PFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQIT 258
            +LG +K VV G+GPLGC+P   A       +C++ +N  V+ +N  L  ++  LN ++ 
Sbjct: 213 HQLGGRKFVVVGVGPLGCIPFARALNLIPAGKCSEQVNQVVRGYNMKLIHSLKTLNNELR 272

Query: 259 KHQHYSSSSSSKIFILDVYDAFLSIIQGRGSGSGSGRVGLLKFKTPLKPCCFGVSSEFEC 299
                S   ++     + YD FL ++             L   K   KPCC G    F C
Sbjct: 273 -----SEDYNTTFVYANSYDLFLKLVL---------NYQLFGLKNADKPCCGGYFPPFAC 317

The following BLAST results are available for this feature:

BLAST of Cucsat.G1549 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZS7	8.1e-83	54.76	GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2...	[more]
Q9SF94	7.9e-70	48.79	GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2...	[more]
Q9LZS8	8.2e-51	39.65	GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3...	[more]
Q8RWJ4	4.5e-49	41.81	GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2...	[more]
Q9LZS9	1.7e-48	40.14	GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3...	[more]

BLAST of Cucsat.G1549 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143379.2	3.35e-209	98.01	GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protei...	[more]
XP_008462669.1	1.30e-184	87.66	PREDICTED: GDSL esterase/lipase At5g03610-like, partial [Cucumis melo]	[more]
TYK07397.1	1.35e-184	87.66	GDSL esterase/lipase [Cucumis melo var. makuwa]	[more]
KAA0025271.1	2.50e-184	87.66	GDSL esterase/lipase [Cucumis melo var. makuwa]	[more]
XP_038883594.1	8.34e-165	81.79	GDSL esterase/lipase At5g03610-like [Benincasa hispida]	[more]

BLAST of Cucsat.G1549 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CJ29	6.31e-185	87.66	GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=3...	[more]
A0A5D3C686	6.54e-185	87.66	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202...	[more]
A0A5A7SLZ4	1.21e-184	87.66	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541...	[more]
A0A6J1EC94	2.98e-137	68.23	GDSL esterase/lipase At5g03610-like isoform X3 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1ECT1	1.34e-136	68.35	GDSL esterase/lipase At5g03610-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of Cucsat.G1549 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03610.1	5.8e-84	54.76	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT3G09930.1	5.6e-71	48.79	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G03600.1	5.8e-52	39.65	SGNH hydrolase-type esterase superfamily protein	[more]
AT2G36325.1	3.2e-50	41.81	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G41890.1	3.8e-27	28.67	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 18..261 e-value: 2.3E-20 score: 74.1
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 3..300 e-value: 9.4E-47 score: 161.9
None	No IPR available	PANTHER	PTHR46020	OSJNBB0059K02.9 PROTEIN	coord: 8..301
None	No IPR available	PANTHER	PTHR46020:SF4	OSJNBB0059K02.9 PROTEIN	coord: 8..301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G1549.T1	Cucsat.G1549.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds