Cucsat.G15452 (gene) Cucumber (B10) v3

Overview
NameCucsat.G15452
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionMetal-nicotianamine transporter like
Locationctg2009: 5604651 .. 5609006 (-)
RNA-Seq ExpressionCucsat.G15452
SyntenyCucsat.G15452
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGTTTTAATTTTTAGAGCTACCCATGTGAAAAAAAAAAAAAAAAAATTAACTTTTTGTTGGGTTTTGATTGTTTAGAAGATTAATTAAGTGATGAATCTTTTCGTAATTACAACGTGGAGGAAACAAATTAAAGATCTGTTCAACATCTACCTGAAGAAAAAGAAAAAGAAAAAAGAAAAACCAATTCCACAGTTGTCACAGCACACACTCCAACGAATCAAATCCTTTTCTAATCAACATCGTGGATCACACATCCCAATAAACGCATCGAAAGGAGGATTCGTTCTGGATTAAAAGGGAATTCTATTGCGATTGAATCCCGTGGAAAAAGGAAGAATTTGAGACGAATCATCTCAATGGGGACCGAGAAAACTCGCGTAGAGATATCAGAACCGTTGTTGGTGGAGCCTGAGAATCATGGAGGGTCCGTTGAATCTGATGATTTGGAAGAAATCCCAGATTGGAAAGATCAAATCACGATCAGAGGATTGGCTGTGAGTGCTTTGTTAGGGACTTTGTTCTGTATCATTACCCACAAGCTGAATCTAACAGTGGGAATCATCCCGTCCTTGAACGTCGCTGCTGGATTATTGGGTTTCTTCTTCGTCAAATCATGGACTGGGGTTTTGTCCAAGATTGGTTTCTCAGTTGTACCATTTACCAAGCAAGAGAACACCGTAATCCAGACATGCGTTGTGGCTTGTTACGGCCTGGCCTTCAGTGGTAATCTCTCTCTCTCTCTCTCTCTTTTTTCTTCTTTGTCGCTGACCATTTATTTTACATAAGCTGTCTTCTTGTGCTTTCGGGATTACTCTATAGGGATTCTCTGTTACTTTGTAAAATTTGTACGTCTTTCTTCAATTTCTGAAGCCATGTAGCGTTTTGGTATTAGTGTTCTTGGAGTCTTCTACTTTTATCTAGCAAATTCAGATACATCCCCTTTCATTGCCCTAGAAACTAGAAAGTGAAATTGGGAGAGAAAAGTCTATGACTTGATTTTTCTACTGCCAGATTGTTCAATAGGGGGATGCACTTCAATGCATTGGGCTTGGTTTGACTTACATCGACCATTTTTATATTTATTTTTAAAAAGGAAACAGAACTTTTAGTTGAAAAAAATGAAAGGAGATTAATGCTAAAAAAAAGTAACATTCAAAAGAAGAAAGAAATGAGAAACTGAGAAAAACAAAAGAAGACAATACGAGTGAGATACAAAGAGGAAGAAGTCATCAAAAAAAGCAAACGCTTAAAACTTAACAAACCAACATCAGAAAAGAAACCAAAGCTAGAGACCACATAAACTTTAAGGTCAAAGCCCACTAAAGACGGATGAGGAGATGAGGAAAAAAGTCCTTTAATGACTAGAGATTCAGAAATGTGAGGATCAAACCATAATGAAAATCAACTTTTAAACTGTCTATTACCAGCTTGCGACTACTAGGAAAAAAACACAACTTCTTAAGGGCAAAATAGTACGGTTTAGTTGCACGAATGTGAATTCACAATAATGATTCCATTTCTAGACTAAACAACGAAGCTAGACTTCCAACAAGGTGGAATTGATTTCATTTGCAAACCACATTTATCAATCAAAGCAGAGAATTTGGGAATTATAGATGACATTTCTGCTTGAATATTTACACTATTCTAAAACAGGTATCAAAACCATGTGTGAAGTAAACCTCTAAATTTTCTTCAACTAAATCACCTTATGCTAGAGAGTCCATAATATCCTCACAGCTTACTCCATAAGCTGAATCTCCATGAGCAGCTTTTTAATATCAATCTCAATACTCTGGACAAGACTGTTACAAGTTTTCTAAGCATTCATGTGCAGGATTCAAGTTAAGCTAATATCTTGGTGTCCCTGTGTGCTTGATTTTTGATTTACACGAACTTAACTATTTAATCCTCGAAGTTGTTATTGGATTTTAGAAGTTATATATTATAACTTTGCTTTCTCTTAACTGGCTTTGTGGAATGCTGCCTGGAACTGCATCATTGCATCTACAAACCTATTAGTACAAAAAAAGAAAAAAACCTGGCTGTTAGTCTGTGGCTTGTATCTCTATTAGTTGATTTCTCCTCTACTTTTTTTTCCCTGTTTGAGCTGTATACTTTTTCTTCCCCTAGTAACTTTTCTTGGGGTTTGTTGATGATTAACAGAATCTCACATTGTATCATTGCCAATTTTAGAATTTTTTTCTTGCTTGGAATTCATGGTGTTGTCTTTGTCCATTTTATGTTGTCAGCATCTACAGGTTGACTTGGTAAAGAAACAAATGCATTCATCCTCGTTTAGAAACATAATTTGAACTGAGTTTCCCTCCAACTTACTTGTATTATGAGCAGGAAACTTTGACCCTCGTGGGTTCCCTATGTTTTCCTGTTTAATCCAATTGATTAATTTTTATATCATTTCAGGAGGATTTGGTTCTTATATAATTGCCATGGATGAGAGAACATATAAGTTAATTGGCACAGATTATCCTGGTAACCGGGCAGAAGATGTGATAAACCCAGGACTGGCGTGGATGATTGGATTTTTGTTTGTTGTCAGTTTCCTTGGGCTATTCAGTCTTGTGCCTCTGCGTAAGGTGACTGTTATAGGCTTTTGTTGATATTCAACACATCATTATACTGTTGAATGCGTTCAACTTAAACCTTCTGATTTTCTCTCTTCTTTTTTTGATTTTTTACTTCTGTTTTCTGGCTGGTTGAGAAATAAATGAAAACTCATATCCTTACTTGCCATTCTACAATTCATTTGGTCAAAAGATACCACTAAAGCTTGTTATTGCCTGAACCAATTACCCACTACCAGAGGAATCTATCTTTTTTCTACAGTTAGTTCATTTCATGCATGATCTGCTTCTCTGAAAATCGTCAATTAGTGCACTTTGAGGAGTGACCTAGAGATGAAAACATATTTCCATGGAACTCTATATTACATTTTGCATTAGATTGGCTGCTCAAATAAGTGAAGTAAAATATTCAACGCCTAGGGCCTGCAGAAAAAATGAAAGAGGGGAAGAATAAGCTCAAATCCCATGTTGTGAAATTTTACGAGTTTCCTGAAAGATCTTGTGAAATTTTTCGAAGTGCATAAATTGAATTAGTCATTCGCAGACATTGACAAAAAGAAAAAATGTTGTGCTTTCTCTTCCCTTTTTTTTTTAGAAAAAACTCTTGTTGGATGAGTCCAGGTGATGGTCTTGGATTACAAGCTTACATATCCCAGCGGAACAGCCACGGCCATGCTGATAAATAGTTTCCATACTAACACAGGAGCTGAGCTTGCAGGGTAAACACATTGTTGAATCTAAAGATAAATGGGAATGGGATCTTTTGTTGTGCCCAAGGCCCATTGTAATCTTCCAATAATATTTTAGTTGCTGTAAATATTAAGTGGATGGTCCCCTTTGCAGCTCACACCTCCATTCTTTTTTTTTTTTGTTTTTTTTTTTTTTTTTGCAGAAAGCAGGTACATTGTCTTGGAAAATATCTCGGGATAAGTTTCATTTGGAGCTGTTTCAAGTGGTTCTTCAGTGGTGTGGGAGATTCTTGTGGATTTGATAATTTTCCCACTCTTGGCTTGGAATTATATAAGAATACGTAAGTCTTGAATTCTCTTGAACCTTTTTCTTCTTTCCAAAAAGAAGGAAGGAAGAATAGAAAATTAAATATGTTAAACTAGACATGTTCATAAACAGAATCATTGCATTATTTACTCATGTCTCCTTTTTCTTTTTCTTCTCTTATTGGTCTAAGAAGGAAGAAGATGCTACAAATTGAATCTAAGTTTTTCAAACTCAGAGTCCTAATTATTTCTATTACAACGGAAGCAAATTGAAAAATGTTTCTCACCTTCTCACTTTTAGAAATTCAATTAAATTATATAGGATGTCTGGGTAGTGATGGTCGATTGATGAGATCAAATCACACCTAAAACTGCAGTACTATACCAAGAATACATGGACAACCCATTAATAATTTCGTATTGACCTTTTTGCTAGCTTTGATGAGAAAAAAATACTGTGGAATTGGTTTTCCTCAGAATTTATGTTCCTCTCCCCAATCCCCATCATCTCTTGAATCTATAGGTTTCATCAAAAGTAGATATTTTATCCATTTGGCTTGTCTTAATTTTCTACAAATTATTGTTATAAATATTGCCTCCACTAAATGTTATATTACTCTACCCAGACTATTATGTAAATTTATGCAATTAATTACTTATTTTCTTGCAGTTTTTATTTCGACTTTAGTCCAACTTATGTGGGTTGCGGCCTCATTTGTCCTCACATTGTAAATTGCTCTGTTCTTCTTGGAGCAATTATATCATGGGGGTTTCTTTGGCCATTTGTTACACGGCATGCTGGGGACTGGTATCCAGCTGACCTTGGGAGCAATGACTTTAAAGGACTTTATGGATATAAGGTACTTGAAGTTTTGACTGTCTAGATTACGTGTCGTTCTTTGAGACATTGGTTGACAATGATTCTTTTAGGTTTATGTGTTATCCAATGAATATGTTTGTGTTCGCCTCCCATTGTCTTGCAACATTCTAGACTATATGTTATGGCTCCAGTCAATTTTAATTTTAGAGTGTGAAAATGAAAACTGTTCACATGTTTCAACTGTGTTCTTTGTTGTGAGATCTTTAAGGAGATAAATAATAATGTTTATAGGTGTGGAGAGAGGCCCTAGTGACATTTGGTTCCTTATTAGATTTCGTGTTTCTCTATGGACGTCAATTTATTTTTGCATAGTTGGAACCTTGAATGGGCCCTTTTTGTGGGCTTGGTTTTTTCGTATATGCTTGTATTCTTTCATTTTTTTTCTCAATGACCTCTTGCATCCCGATGATTTTGTTGTCTGCCTCAAACTGCTTTGGTTATTGTTTCTCTGCAGGTCTTCATAGCTATTTCCCTCATCCTTGGTGACGGTCTTTATAATTTGATCAAAATTATATACCTTACTGTGAAAGAAATTTGCAACAAAAGCACCACAAATAATAACCTCCCCGTCATAAAAGAAGCCACAGGTTGGTATTTTACCTTGGAGTTGTTCGAGTTGATTATATGTTACTTCCTCCTAGATTTCTTTCACTTGCTTTTGTTGCATAAGCCTTCCATTTTGAATGGTTGTCATAAATAATTATGACAATCAACAACATTGAAAAGTTTTAATGGAATATTGTTAGTTTCTTAGCGCAAAGTCTGGTTATGTAGGCAATGAGTCCAGTGACATCATATCCAGATTCCTAGTTAGTTAATAAACTGATTTGCTGAGATGCTCATTTCATCAGTGTTTTATGGCTTATCAATTGATTGATCAAATGCGTAGTCTTATTACGACATATATGAAGACTGAATAAGACTTGCATATTTGAGGAAAAATGGTGTTGTGGGCATTCTGTTTTTGGATTTTATTGTGTCTGTTTGACATAAAATGCTTAGGTTCTCCCAAAAACTTTTACTATCAATAGTTTGTTAGCTCAAATGGATTTAATGGTCATGAACTGTCCTGATGTCTAAATTGGCAGATAGCGAGGGGTATAAATTGCTTGCCGAGGAAAGAAGGAAGGATAAGGTATTTCTTAAAGATAGAATACCGACATGGTTTGCAGCATCTGGATATGTTGGCCTAGCAGCGATATCGACAGCAACAATGCCAATGATCTTCCCACCCTTGAAGTGGTATTTGGTTCTTGGCGCTTATGTTGTTGCACCTGCCCTTGCTTTCTGCAACTCCTACGGCACTGGTCTTACTGACTGGAATCTATCATCAACTTATGGGAAGCTTGGTCTTTTTCTATTTGCTTCTTTAGTTGGAGGCAATGGCGGAGTTATTGCTGGATTAGCAGCTTGCGGTGTTATGATGTCAATTGTTTCAACAGCTGCTGATCTTATGCAAGACTTCAAGACGGGTTATCTTACTCTTTCTTCAGCCAAGTCTATGTTTGTAAGTCAGCTAGTAGGGACAGCCATGGGCTGTGTGATTGCTCCCCTTACATTTTGGTTATTTTGGAGCGCTTTCGATATCGGGTTACCTGATAGTCCATACAAAGCCCCTTATGCTGTAATATTTAGAGAAATGGCCATTCTAGGTGTTGAGGGTTTTTCAGAGCTCCCCAAACATTGTTTGGCCATGTGTTGTGGGTTTTTTGTGGCAGCTTTCTTAGTTAACCTTTTGAGGGACGTGGTTCCAAAGAAAATCGCACAATTCATTCCAATTCCAATGGCCATGGCTGTTCCATTTTACATTGGAGCTTACTTTGCTATCGATATGTTTGTTGGGACTGTCATCCTCTTTATATGGGAGCGAATTAATCGGAAGGATGCAGAGGATTATGCTGGTGCAGTTGCGTCGGGTTTGATATGTGGTGACGGAATATGGACAATTCCTTCAGCAGTGCTATCAATTTTCAGGATCAATCCTCCCATTTGCATGTCATTCAACCCTACAGTTGCCTGAGCTACATCATTGAACGCTAGCTCACATTGTACACAATTTAATGAAACAAAGCTACTGATTTATATTTTACTTTGTATATACAGATCCTTGTCATTTAAATTCTAAGATAGTCTTTATTTTCTCAACGTCCTTGATGTTCCAAGGAAAGACTTCACATCATTTCATTTTTTAAGGCAAAATACCAATTATTTAAGGTCCTATTTGTCTGCGTTCTATGATATATATATATGACCATGTTTCATAGCATATTT

Coding sequence (CDS)

ATGGGGACCGAGAAAACTCGCGTAGAGATATCAGAACCGTTGTTGGTGGAGCCTGAGAATCATGGAGGGTCCGTTGAATCTGATGATTTGGAAGAAATCCCAGATTGGAAAGATCAAATCACGATCAGAGGATTGGCTGTGAGTGCTTTGTTAGGGACTTTGTTCTGTATCATTACCCACAAGCTGAATCTAACAGTGGGAATCATCCCGTCCTTGAACGTCGCTGCTGGATTATTGGGTTTCTTCTTCGTCAAATCATGGACTGGGGTTTTGTCCAAGATTGGTTTCTCAGTTGTACCATTTACCAAGCAAGAGAACACCGTAATCCAGACATGCGTTGTGGCTTGTTACGGCCTGGCCTTCAGTGGAGGATTTGGTTCTTATATAATTGCCATGGATGAGAGAACATATAAGTTAATTGGCACAGATTATCCTGGTAACCGGGCAGAAGATGTGATAAACCCAGGACTGGCGTGGATGATTGGATTTTTGTTTGTTGTCAGTTTCCTTGGGCTATTCAGTCTTGTGCCTCTGCGTAAGGTGATGGTCTTGGATTACAAGCTTACATATCCCAGCGGAACAGCCACGGCCATGCTGATAAATAGTTTCCATACTAACACAGGAGCTGAGCTTGCAGGAAAGCAGGTACATTGTCTTGGAAAATATCTCGGGATAAGTTTCATTTGGAGCTGTTTCAAGTGGTTCTTCAGTGGTGTGGGAGATTCTTGTGGATTTGATAATTTTCCCACTCTTGGCTTGGAATTATATAAGAATACTTTTTATTTCGACTTTAGTCCAACTTATGTGGGTTGCGGCCTCATTTGTCCTCACATTGTAAATTGCTCTGTTCTTCTTGGAGCAATTATATCATGGGGGTTTCTTTGGCCATTTGTTACACGGCATGCTGGGGACTGGTATCCAGCTGACCTTGGGAGCAATGACTTTAAAGGACTTTATGGATATAAGGTCTTCATAGCTATTTCCCTCATCCTTGGTGACGGTCTTTATAATTTGATCAAAATTATATACCTTACTGTGAAAGAAATTTGCAACAAAAGCACCACAAATAATAACCTCCCCGTCATAAAAGAAGCCACAGATAGCGAGGGGTATAAATTGCTTGCCGAGGAAAGAAGGAAGGATAAGGTATTTCTTAAAGATAGAATACCGACATGGTTTGCAGCATCTGGATATGTTGGCCTAGCAGCGATATCGACAGCAACAATGCCAATGATCTTCCCACCCTTGAAGTGGTATTTGGTTCTTGGCGCTTATGTTGTTGCACCTGCCCTTGCTTTCTGCAACTCCTACGGCACTGGTCTTACTGACTGGAATCTATCATCAACTTATGGGAAGCTTGGTCTTTTTCTATTTGCTTCTTTAGTTGGAGGCAATGGCGGAGTTATTGCTGGATTAGCAGCTTGCGGTGTTATGATGTCAATTGTTTCAACAGCTGCTGATCTTATGCAAGACTTCAAGACGGGTTATCTTACTCTTTCTTCAGCCAAGTCTATGTTTGTAAGTCAGCTAGTAGGGACAGCCATGGGCTGTGTGATTGCTCCCCTTACATTTTGGTTATTTTGGAGCGCTTTCGATATCGGGTTACCTGATAGTCCATACAAAGCCCCTTATGCTGTAATATTTAGAGAAATGGCCATTCTAGGTGTTGAGGGTTTTTCAGAGCTCCCCAAACATTGTTTGGCCATGTGTTGTGGGTTTTTTGTGGCAGCTTTCTTAGTTAACCTTTTGAGGGACGTGGTTCCAAAGAAAATCGCACAATTCATTCCAATTCCAATGGCCATGGCTGTTCCATTTTACATTGGAGCTTACTTTGCTATCGATATGTTTGTTGGGACTGTCATCCTCTTTATATGGGAGCGAATTAATCGGAAGGATGCAGAGGATTATGCTGGTGCAGTTGCGTCGGGTTTGATATGTGGTGACGGAATATGGACAATTCCTTCAGCAGTGCTATCAATTTTCAGGATCAATCCTCCCATTTGCATGTCATTCAACCCTACAGTTGCCTGA

Protein sequence

MGTEKTRVEISEPLLVEPENHGGSVESDDLEEIPDWKDQITIRGLAVSALLGTLFCIITHKLNLTVGIIPSLNVAAGLLGFFFVKSWTGVLSKIGFSVVPFTKQENTVIQTCVVACYGLAFSGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKVMVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGDSCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRHAGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPVIKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLVLGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSIVSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYKAPYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAMAVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIFRINPPICMSFNPTVA
Homology
BLAST of Cucsat.G15452 vs. ExPASy Swiss-Prot
Match: Q6R3K6 (Probable metal-nicotianamine transporter YSL6 OS=Arabidopsis thaliana OX=3702 GN=YSL6 PE=2 SV=2)

HSP 1 Score: 975.3 bits (2520), Expect = 3.0e-283
Identity = 465/556 (83.63%), Postives = 515/556 (92.63%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSY+IAMDE+TYKLIG DYPGN AEDVINPGL WMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 120 SGGFGSYLIAMDEKTYKLIGADYPGNHAEDVINPGLWWMIGFLFVVSFLGLFSLVPLRKV 179

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAG QV CLGKYL +S IWSCFKWFFSG+GD
Sbjct: 180 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGNQVKCLGKYLSLSLIWSCFKWFFSGIGD 239

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           +CGFDNFPTLGL L+KNTFYFDFSPTY+GCGLICPHIVNCSVLLGAIISWG LWPFV++H
Sbjct: 240 ACGFDNFPTLGLTLFKNTFYFDFSPTYIGCGLICPHIVNCSVLLGAIISWGILWPFVSQH 299

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAI++ILGDGLYNL+KII +TVKE+C+  +   NLP+
Sbjct: 300 AGDWYPADLGSNDFKGLYGYKVFIAIAIILGDGLYNLVKIIAVTVKELCSSRSRRLNLPI 359

Query: 260 IKEAT-DSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYL 319
           + +   DSE  ++L  ++++D+VFLKDRIP  FA +GYVGLAAISTAT+P+IFPPLKWY 
Sbjct: 360 VTDGVDDSEASEILLVKKKRDEVFLKDRIPLEFAIAGYVGLAAISTATIPIIFPPLKWYF 419

Query: 320 VLGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMS 379
           VL +Y +APALAFCNSYGTGLTDW+L+STYGK+GLF+ AS+VG +GGVIAGLAACGVMMS
Sbjct: 420 VLCSYFIAPALAFCNSYGTGLTDWSLASTYGKIGLFIIASVVGSDGGVIAGLAACGVMMS 479

Query: 380 IVSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPY 439
           IVSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFW+AFDIG P+ PY
Sbjct: 480 IVSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWTAFDIGDPNGPY 539

Query: 440 KAPYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMA 499
           KAPYAVIFREMAILG+EGF+ELPKHCLA+C GFF+AA +VNLLRD+ P KI+QFIPIPMA
Sbjct: 540 KAPYAVIFREMAILGIEGFAELPKHCLALCYGFFIAALIVNLLRDITPPKISQFIPIPMA 599

Query: 500 MAVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSI 559
           MAVPFYIGAYFAIDMFVGTVILF+WERINRKDAED+AGAVASGLICGDGIWTIPSA+LSI
Sbjct: 600 MAVPFYIGAYFAIDMFVGTVILFVWERINRKDAEDFAGAVASGLICGDGIWTIPSAILSI 659

Query: 560 FRINPPICMSFNPTVA 575
            RINPPICM F P+ A
Sbjct: 660 LRINPPICMYFGPSSA 675

BLAST of Cucsat.G15452 vs. ExPASy Swiss-Prot
Match: Q6R3K8 (Probable metal-nicotianamine transporter YSL4 OS=Arabidopsis thaliana OX=3702 GN=YSL4 PE=2 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 2.3e-272
Identity = 443/554 (79.96%), Postives = 504/554 (90.97%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSY+IAMDERTYKLIG+DYPGN  EDVINPGL WM GFLFVVSFLGLF LVPLRKV
Sbjct: 117 SGGFGSYLIAMDERTYKLIGSDYPGNNPEDVINPGLWWMTGFLFVVSFLGLFCLVPLRKV 176

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           M+LDYKLTYPSGTATAMLINSFH NTGAELAGKQV CLGKYL +S +WSCFKWFFSG+G 
Sbjct: 177 MILDYKLTYPSGTATAMLINSFHNNTGAELAGKQVKCLGKYLSLSLVWSCFKWFFSGIGG 236

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           +CGFD+FPTLGL L+KNTFYFDFSPT++GCG+ICPH+VNCSVLLGAIISWGFLWPF+++H
Sbjct: 237 ACGFDHFPTLGLTLFKNTFYFDFSPTFIGCGMICPHLVNCSVLLGAIISWGFLWPFISQH 296

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADL +NDFKGLYGYKVFIAIS+ILGDGLYNLIKII +TVKEICNKS+  +NLPV
Sbjct: 297 AGDWYPADLKANDFKGLYGYKVFIAISIILGDGLYNLIKIIVVTVKEICNKSSRQHNLPV 356

Query: 260 IKEATD-SEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYL 319
             +  D S+   L+ E++++D +FLKDRIP  FA SGYVGLAAISTA +P+IFPPLKWY 
Sbjct: 357 FTDILDKSKTSVLMREKKKRDIIFLKDRIPLEFAVSGYVGLAAISTAIIPLIFPPLKWYF 416

Query: 320 VLGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMS 379
           VL +Y+VAP LAFCNSYG GLTD ++ STYGK GLF+ AS+VG NGGVIAGLAACG+MMS
Sbjct: 417 VLCSYLVAPGLAFCNSYGAGLTDMSMPSTYGKTGLFIVASIVGNNGGVIAGLAACGIMMS 476

Query: 380 IVSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPY 439
           IVSTAADLMQDFKTGYLTLSSAKSMFV+QL+GTAMGC+IAPLTFWLFW+AFDIG PD  Y
Sbjct: 477 IVSTAADLMQDFKTGYLTLSSAKSMFVTQLLGTAMGCIIAPLTFWLFWTAFDIGDPDGLY 536

Query: 440 KAPYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMA 499
           KAPYAVI+REMAILGVEGF++LPKHCLA+CCGFF+AA +VNL+RD+ P KI++ IP+PMA
Sbjct: 537 KAPYAVIYREMAILGVEGFAKLPKHCLALCCGFFIAALIVNLIRDMTPPKISKLIPLPMA 596

Query: 500 MAVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSI 559
           MA PFYIGAYFAIDMFVGTVI+ +WER+N+KDA+DY+GAVASGLICGDGIWTIPSA+LSI
Sbjct: 597 MAGPFYIGAYFAIDMFVGTVIMLVWERMNKKDADDYSGAVASGLICGDGIWTIPSAILSI 656

Query: 560 FRINPPICMSFNPT 573
            RINPPICM F P+
Sbjct: 657 LRINPPICMYFRPS 670

BLAST of Cucsat.G15452 vs. ExPASy Swiss-Prot
Match: Q7XRV2 (Probable metal-nicotianamine transporter YSL6 OS=Oryza sativa subsp. japonica OX=39947 GN=YSL6 PE=2 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 1.1e-269
Identity = 435/554 (78.52%), Postives = 501/554 (90.43%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSY++AMD++TY+LIG DYPGNRA DV+NP L WMIGF+FVVSFLGLFSLV LRKV
Sbjct: 123 SGGFGSYMLAMDQKTYELIGPDYPGNRAIDVMNPSLGWMIGFMFVVSFLGLFSLVALRKV 182

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MV+DYKLTYPSGTATAMLINSFHT +GAELA KQV CLGKYL ISF W+CFKWFFSGVGD
Sbjct: 183 MVIDYKLTYPSGTATAMLINSFHTTSGAELAEKQVSCLGKYLSISFFWNCFKWFFSGVGD 242

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFP+LGL  +KNTFYFDFSPTY+GCGLICPHIVNCS LLGAIISWGFLWP+++  
Sbjct: 243 SCGFDNFPSLGLAAFKNTFYFDFSPTYIGCGLICPHIVNCSTLLGAIISWGFLWPYISTK 302

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPA+LGSNDFKGLYGYKVFI++S+ILGDGLYNLIKIIY T+KE+ N  +    LP+
Sbjct: 303 AGDWYPANLGSNDFKGLYGYKVFISVSVILGDGLYNLIKIIYATIKEVMNARSKQGRLPL 362

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           ++   D EG KL AEE+ ++  FLKDRIP+W A SGYVGLAAISTAT+PMIFP +KWYLV
Sbjct: 363 VRVHDDDEGSKLSAEEKLRNDTFLKDRIPSWLAGSGYVGLAAISTATVPMIFPQVKWYLV 422

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           L AYVVAP LAFCNSYG GLTDWNL+STYGK+GLF+FASLVG +GGVIAGLAACGVMMSI
Sbjct: 423 LCAYVVAPLLAFCNSYGCGLTDWNLASTYGKIGLFIFASLVGRSGGVIAGLAACGVMMSI 482

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDF+TGYLTLSS +SMFVSQL+GT +GC+IAPLTFWL+W+AFDIG PD  +K
Sbjct: 483 VSTAADLMQDFRTGYLTLSSPRSMFVSQLIGTTLGCIIAPLTFWLYWTAFDIGNPDGMFK 542

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVI+REM+ILGVEGFS LP+HCLA+C  FFVAA L+NLLRDV PK +++FIP+PMAM
Sbjct: 543 APYAVIYREMSILGVEGFSALPQHCLAICSVFFVAAILINLLRDVTPKSVSKFIPLPMAM 602

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILF+WER+NRK++ED+AGA+ASGLICGDGIW++PSA+LSI 
Sbjct: 603 AVPFYIGAYFAIDMFVGTVILFVWERVNRKESEDFAGAIASGLICGDGIWSVPSAILSIM 662

Query: 560 RINPPICMSFNPTV 574
           RI+PP+CM F P++
Sbjct: 663 RIDPPMCMYFKPSL 676

BLAST of Cucsat.G15452 vs. ExPASy Swiss-Prot
Match: Q7XRV1 (Probable metal-nicotianamine transporter YSL5 OS=Oryza sativa subsp. japonica OX=39947 GN=YSL5 PE=2 SV=3)

HSP 1 Score: 814.7 bits (2103), Expect = 6.7e-235
Identity = 371/555 (66.85%), Postives = 467/555 (84.14%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SG   SYI AMD +TY+L+G DYPGNR ED+ +P L WMIGF+F+++ +G FS+V LRKV
Sbjct: 112 SGCSSSYIFAMDRKTYELVGQDYPGNRMEDIRDPSLGWMIGFMFLIALIGPFSIVMLRKV 171

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MV+DYKL +P GTATA++INS H  T A+LAG++VHCL KY+ +SF WS FKWFFSGVGD
Sbjct: 172 MVIDYKLAFPGGTATALMINSLHGKTEADLAGRKVHCLVKYMSLSFGWSFFKWFFSGVGD 231

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFP+ G+E +KNTFYF+F+P+YVG GLI PHIVNCSV LG++ISWGFLWPF+ + 
Sbjct: 232 SCGFDNFPSFGIEAFKNTFYFNFNPSYVGYGLISPHIVNCSVFLGSVISWGFLWPFIAKQ 291

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYP +L + DF+GLYGYKVFIAIS+ILGDGLYNL+K+  +  KEICN  +  ++LPV
Sbjct: 292 AGDWYPDNLSNTDFRGLYGYKVFIAISVILGDGLYNLVKVFLIIAKEICNARSKEHDLPV 351

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
                D +  + L +E+R+ ++FLKD IPTW A SGY+ LAAIST  +P+IFP LKWYLV
Sbjct: 352 QALLQDDDSSRQLLDEKRQTEIFLKDSIPTWLAVSGYIVLAAISTVAVPIIFPQLKWYLV 411

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           L  Y +APA+AFCNSYG GLT+ NL+ TYGK+ LF+FASLVG +GGVIAGLAACGV+MSI
Sbjct: 412 LVCYFLAPAIAFCNSYGMGLTNLNLAPTYGKIALFVFASLVGSDGGVIAGLAACGVIMSI 471

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           V + ADLMQDFK+GYLTLSS +SMF+SQ++G A+GC+IAPLT WLFW+AFDIG PD  YK
Sbjct: 472 VCSTADLMQDFKSGYLTLSSPRSMFISQMIGVALGCIIAPLTLWLFWTAFDIGDPDGEYK 531

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           AP+A+IFREMAI+G+EGF+ LP+HCL +CC FF+AA ++NL++DVVP  +++FIPIPMAM
Sbjct: 532 APFAIIFREMAIIGIEGFAALPRHCLEICCVFFLAALIINLMKDVVPNHVSRFIPIPMAM 591

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYF +DMF+GT+ILF W++I+R++A+DYA AVASGLICGDG+W+IPSAVLSI 
Sbjct: 592 AVPFYIGAYFGVDMFIGTLILFAWQKIDRREADDYAVAVASGLICGDGVWSIPSAVLSIL 651

Query: 560 RINPPICMSFNPTVA 575
            ++PPICMSF P+ A
Sbjct: 652 GVDPPICMSFRPSSA 666

BLAST of Cucsat.G15452 vs. ExPASy Swiss-Prot
Match: Q5JQD7 (Probable metal-nicotianamine transporter YSL12 OS=Oryza sativa subsp. japonica OX=39947 GN=YSL12 PE=2 SV=2)

HSP 1 Score: 702.2 bits (1811), Expect = 4.8e-201
Identity = 336/553 (60.76%), Postives = 419/553 (75.77%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFG+Y+  M E   K        N A++V NP + WMIGFLF+VSF+GL +LVPLRK+
Sbjct: 154 SGGFGTYLFGMSETIAK---QATEANNAQNVKNPHIGWMIGFLFLVSFIGLLALVPLRKI 213

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           M++DYKLTYPSGTATA LIN FHT  GA+LA KQV  LGK+   SF+W  F+WF++  GD
Sbjct: 214 MIVDYKLTYPSGTATAYLINGFHTPEGAKLAKKQVKELGKFFLFSFVWGFFQWFYT-AGD 273

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
            CGF +FPTLGL+ YKN FYFDFSPTYVG G+ICPHIVN SVLLG I+SWG +WP +   
Sbjct: 274 GCGFQSFPTLGLQAYKNRFYFDFSPTYVGVGMICPHIVNVSVLLGGILSWGIMWPLIRNK 333

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
            G WY A L      GL GY+VFI+I+LILGDGLYN +K++  T          N+ LPV
Sbjct: 334 KGSWYAASLSETSLHGLQGYRVFISIALILGDGLYNFVKVLIRTTAGFVVMMKKNSTLPV 393

Query: 260 IKEATDSEGYKLLA-EERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYL 319
               +     + ++ ++ R+ ++FLKD+IP   A  GYV +AA+S  T+P IFP LKWY 
Sbjct: 394 SNNGSPMVATEAISFDDERRTELFLKDQIPKTVAFGGYVAVAAVSIGTLPQIFPQLKWYY 453

Query: 320 VLGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVG-GNGGVIAGLAACGVMM 379
           +L AYV AP LAFCN+YG GLTDW+L+STYGKL +F+F +  G  NGGV+ GLAACGVMM
Sbjct: 454 ILVAYVFAPVLAFCNAYGAGLTDWSLASTYGKLAIFIFGAWAGASNGGVLVGLAACGVMM 513

Query: 380 SIVSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAF-DIGLPDS 439
           SIVSTA+DLMQDFKTGYLTL+S +SMFVSQ++GTAMGCVIAP  FWLF+ AF DIG+  +
Sbjct: 514 SIVSTASDLMQDFKTGYLTLASPRSMFVSQVIGTAMGCVIAPCVFWLFYKAFADIGVSGT 573

Query: 440 PYKAPYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIP 499
            Y APYA+++R MAILGV+GFS LPKHCL +C  FF AA  +NL RD+ P K+A+FIP+P
Sbjct: 574 EYPAPYAIVYRNMAILGVDGFSSLPKHCLTLCYIFFAAAIAINLARDLAPSKVARFIPLP 633

Query: 500 MAMAVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVL 559
           MAMA+PFYIG+YFAIDMF+GTVILF+WE +N+  AE +A AVASGLICGDGIWT+P ++L
Sbjct: 634 MAMAIPFYIGSYFAIDMFIGTVILFVWEMVNKAKAEAFAPAVASGLICGDGIWTLPQSIL 693

Query: 560 SIFRINPPICMSF 570
           ++ ++ PPICM F
Sbjct: 694 ALAKVKPPICMKF 702

BLAST of Cucsat.G15452 vs. NCBI nr
Match: XP_004148009.1 (probable metal-nicotianamine transporter YSL6 isoform X1 [Cucumis sativus] >KGN58161.1 hypothetical protein Csa_017577 [Cucumis sativus])

HSP 1 Score: 1133 bits (2931), Expect = 0.0
Identity = 555/555 (100.00%), Postives = 555/555 (100.00%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 122 SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 181

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD
Sbjct: 182 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 241

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH
Sbjct: 242 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 301

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV
Sbjct: 302 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 361

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 362 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 421

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI
Sbjct: 422 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 481

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 482 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 541

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM
Sbjct: 542 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 601

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 602 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 661

Query: 560 RINPPICMSFNPTVA 574
           RINPPICMSFNPTVA
Sbjct: 662 RINPPICMSFNPTVA 676

BLAST of Cucsat.G15452 vs. NCBI nr
Match: XP_008450132.1 (PREDICTED: probable metal-nicotianamine transporter YSL6 [Cucumis melo])

HSP 1 Score: 1122 bits (2901), Expect = 0.0
Identity = 550/555 (99.10%), Postives = 552/555 (99.46%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 122 SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 181

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD
Sbjct: 182 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 241

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH
Sbjct: 242 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 301

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKII LTVKEICNKSTTN+NLPV
Sbjct: 302 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIISLTVKEICNKSTTNSNLPV 361

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           IKEATD EGYKLLAEERRKD+VFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 362 IKEATDREGYKLLAEERRKDEVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 421

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVG NGGVIAGLAACGVMMSI
Sbjct: 422 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGDNGGVIAGLAACGVMMSI 481

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 482 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 541

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM
Sbjct: 542 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 601

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 602 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 661

Query: 560 RINPPICMSFNPTVA 574
           RINPPICMSFNPTVA
Sbjct: 662 RINPPICMSFNPTVA 676

BLAST of Cucsat.G15452 vs. NCBI nr
Match: KAA0060249.1 (putative metal-nicotianamine transporter YSL6 [Cucumis melo var. makuwa] >TYJ97613.1 putative metal-nicotianamine transporter YSL6 [Cucumis melo var. makuwa])

HSP 1 Score: 1120 bits (2897), Expect = 0.0
Identity = 549/555 (98.92%), Postives = 551/555 (99.28%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 122 SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 181

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD
Sbjct: 182 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 241

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH
Sbjct: 242 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 301

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAIS ILGDGLYNLIKII LTVKEICNKSTTN+NLPV
Sbjct: 302 AGDWYPADLGSNDFKGLYGYKVFIAISFILGDGLYNLIKIISLTVKEICNKSTTNSNLPV 361

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           IKEATD EGYKLLAEERRKD+VFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 362 IKEATDREGYKLLAEERRKDEVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 421

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVG NGGVIAGLAACGVMMSI
Sbjct: 422 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGDNGGVIAGLAACGVMMSI 481

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 482 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 541

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM
Sbjct: 542 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 601

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 602 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 661

Query: 560 RINPPICMSFNPTVA 574
           RINPPICMSFNPTVA
Sbjct: 662 RINPPICMSFNPTVA 676

BLAST of Cucsat.G15452 vs. NCBI nr
Match: XP_038894292.1 (probable metal-nicotianamine transporter YSL6 [Benincasa hispida])

HSP 1 Score: 1105 bits (2859), Expect = 0.0
Identity = 541/553 (97.83%), Postives = 547/553 (98.92%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 122 SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 181

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD
Sbjct: 182 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 241

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH
Sbjct: 242 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 301

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AG+WYPADL SNDFKGLYGYKVFIAISLILGDGLYNLIKII LTVKEICNKSTT +NLP+
Sbjct: 302 AGNWYPADLESNDFKGLYGYKVFIAISLILGDGLYNLIKIISLTVKEICNKSTTKSNLPL 361

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           IKEATD EGYKL+AEERRKD+VFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 362 IKEATDGEGYKLIAEERRKDEVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 421

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           L AYVVAPALAFCNSYGTGLTDW+LSSTYGKLGLFLFASLVG NGGVIAGLAACGVMMSI
Sbjct: 422 LSAYVVAPALAFCNSYGTGLTDWSLSSTYGKLGLFLFASLVGDNGGVIAGLAACGVMMSI 481

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 482 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 541

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM
Sbjct: 542 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 601

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 602 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 661

Query: 560 RINPPICMSFNPT 572
           RINPPICMSFNPT
Sbjct: 662 RINPPICMSFNPT 674

BLAST of Cucsat.G15452 vs. NCBI nr
Match: KAG6588889.1 (putative metal-nicotianamine transporter YSL6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022654.1 putative metal-nicotianamine transporter YSL6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1088 bits (2813), Expect = 0.0
Identity = 528/553 (95.48%), Postives = 541/553 (97.83%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSY+IAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 121 SGGFGSYLIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 180

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISF WSCFKWFFSGVGD
Sbjct: 181 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFFWSCFKWFFSGVGD 240

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTF+FDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPF+T+H
Sbjct: 241 SCGFDNFPTLGLELYKNTFFFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFITQH 300

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKII LT+KEICNK TT +NLPV
Sbjct: 301 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIISLTIKEICNKRTTRSNLPV 360

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           + E TDSEG KLLAEER+KD+VFLKDRIPTW AASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 361 LTETTDSEGSKLLAEERKKDEVFLKDRIPTWLAASGYVGLAAISTATMPMIFPPLKWYLV 420

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAY+VAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVG NGGVIAGLAACGVMMSI
Sbjct: 421 LGAYIVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGNNGGVIAGLAACGVMMSI 480

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 481 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 540

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGV+GFSELPKHCLAMC GFFVAAFLVN LRD+VPKKIAQFIPIPMAM
Sbjct: 541 APYAVIFREMAILGVQGFSELPKHCLAMCFGFFVAAFLVNFLRDIVPKKIAQFIPIPMAM 600

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 601 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 660

Query: 560 RINPPICMSFNPT 572
           RINPPICM+F PT
Sbjct: 661 RINPPICMTFKPT 673

BLAST of Cucsat.G15452 vs. ExPASy TrEMBL
Match: A0A0A0LDJ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G565320 PE=4 SV=1)

HSP 1 Score: 1133 bits (2931), Expect = 0.0
Identity = 555/555 (100.00%), Postives = 555/555 (100.00%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 122 SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 181

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD
Sbjct: 182 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 241

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH
Sbjct: 242 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 301

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV
Sbjct: 302 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 361

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 362 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 421

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI
Sbjct: 422 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 481

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 482 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 541

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM
Sbjct: 542 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 601

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 602 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 661

Query: 560 RINPPICMSFNPTVA 574
           RINPPICMSFNPTVA
Sbjct: 662 RINPPICMSFNPTVA 676

BLAST of Cucsat.G15452 vs. ExPASy TrEMBL
Match: A0A1S3BNL1 (probable metal-nicotianamine transporter YSL6 OS=Cucumis melo OX=3656 GN=LOC103491805 PE=4 SV=1)

HSP 1 Score: 1122 bits (2901), Expect = 0.0
Identity = 550/555 (99.10%), Postives = 552/555 (99.46%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 122 SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 181

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD
Sbjct: 182 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 241

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH
Sbjct: 242 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 301

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKII LTVKEICNKSTTN+NLPV
Sbjct: 302 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIISLTVKEICNKSTTNSNLPV 361

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           IKEATD EGYKLLAEERRKD+VFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 362 IKEATDREGYKLLAEERRKDEVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 421

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVG NGGVIAGLAACGVMMSI
Sbjct: 422 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGDNGGVIAGLAACGVMMSI 481

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 482 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 541

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM
Sbjct: 542 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 601

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 602 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 661

Query: 560 RINPPICMSFNPTVA 574
           RINPPICMSFNPTVA
Sbjct: 662 RINPPICMSFNPTVA 676

BLAST of Cucsat.G15452 vs. ExPASy TrEMBL
Match: A0A5D3BE81 (Putative metal-nicotianamine transporter YSL6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold639G00070 PE=4 SV=1)

HSP 1 Score: 1120 bits (2897), Expect = 0.0
Identity = 549/555 (98.92%), Postives = 551/555 (99.28%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 122 SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 181

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD
Sbjct: 182 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 241

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH
Sbjct: 242 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 301

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAIS ILGDGLYNLIKII LTVKEICNKSTTN+NLPV
Sbjct: 302 AGDWYPADLGSNDFKGLYGYKVFIAISFILGDGLYNLIKIISLTVKEICNKSTTNSNLPV 361

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           IKEATD EGYKLLAEERRKD+VFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 362 IKEATDREGYKLLAEERRKDEVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 421

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVG NGGVIAGLAACGVMMSI
Sbjct: 422 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGDNGGVIAGLAACGVMMSI 481

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 482 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 541

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM
Sbjct: 542 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 601

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 602 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 661

Query: 560 RINPPICMSFNPTVA 574
           RINPPICMSFNPTVA
Sbjct: 662 RINPPICMSFNPTVA 676

BLAST of Cucsat.G15452 vs. ExPASy TrEMBL
Match: A0A6J1JPT2 (probable metal-nicotianamine transporter YSL6 OS=Cucurbita maxima OX=3661 GN=LOC111486641 PE=4 SV=1)

HSP 1 Score: 1087 bits (2810), Expect = 0.0
Identity = 527/553 (95.30%), Postives = 542/553 (98.01%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSY+IAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 121 SGGFGSYLIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 180

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD
Sbjct: 181 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 240

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTF+FDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPF+T+H
Sbjct: 241 SCGFDNFPTLGLELYKNTFFFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFITQH 300

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKII LT+KEICNK TT +NLPV
Sbjct: 301 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIISLTIKEICNKRTTQSNLPV 360

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           + + TDSEG KLLAEER+KD+VFLKDRIP+W AASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 361 LTKTTDSEGSKLLAEERKKDEVFLKDRIPSWLAASGYVGLAAISTATMPMIFPPLKWYLV 420

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAY+VAPALAFCNSYGTGLTDWNLSSTYGK+GLFLFASLVG NGGVIAGLAACGVMMSI
Sbjct: 421 LGAYIVAPALAFCNSYGTGLTDWNLSSTYGKIGLFLFASLVGNNGGVIAGLAACGVMMSI 480

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 481 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 540

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGV+GFSELPKHCLAMC GFFVAAFLVN LRD+VPKKIAQFIPIPMAM
Sbjct: 541 APYAVIFREMAILGVQGFSELPKHCLAMCFGFFVAAFLVNFLRDIVPKKIAQFIPIPMAM 600

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 601 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 660

Query: 560 RINPPICMSFNPT 572
           RINPPICMSF PT
Sbjct: 661 RINPPICMSFKPT 673

BLAST of Cucsat.G15452 vs. ExPASy TrEMBL
Match: A0A6J1EK77 (probable metal-nicotianamine transporter YSL6 OS=Cucurbita moschata OX=3662 GN=LOC111435107 PE=4 SV=1)

HSP 1 Score: 1085 bits (2805), Expect = 0.0
Identity = 526/553 (95.12%), Postives = 540/553 (97.65%), Query Frame = 0

Query: 20  SGGFGSYIIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 79
           SGGFGSY+IAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV
Sbjct: 121 SGGFGSYLIAMDERTYKLIGTDYPGNRAEDVINPGLAWMIGFLFVVSFLGLFSLVPLRKV 180

Query: 80  MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFIWSCFKWFFSGVGD 139
           MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISF WSCFKWFFSGVGD
Sbjct: 181 MVLDYKLTYPSGTATAMLINSFHTNTGAELAGKQVHCLGKYLGISFFWSCFKWFFSGVGD 240

Query: 140 SCGFDNFPTLGLELYKNTFYFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFVTRH 199
           SCGFDNFPTLGLELYKNTF+FDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPF+T+H
Sbjct: 241 SCGFDNFPTLGLELYKNTFFFDFSPTYVGCGLICPHIVNCSVLLGAIISWGFLWPFITQH 300

Query: 200 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIIYLTVKEICNKSTTNNNLPV 259
           AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKII LT+KEICNK TT +NLPV
Sbjct: 301 AGDWYPADLGSNDFKGLYGYKVFIAISLILGDGLYNLIKIISLTIKEICNKRTTRSNLPV 360

Query: 260 IKEATDSEGYKLLAEERRKDKVFLKDRIPTWFAASGYVGLAAISTATMPMIFPPLKWYLV 319
           + E TDSEG KLLAEER+KD+VFLKDRIPTW AASGYVGLAAISTATMPMIFPPLKWYLV
Sbjct: 361 LTETTDSEGSKLLAEERKKDEVFLKDRIPTWLAASGYVGLAAISTATMPMIFPPLKWYLV 420

Query: 320 LGAYVVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGGNGGVIAGLAACGVMMSI 379
           LGAY+VAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVG NGGVIAGLAACGVMMSI
Sbjct: 421 LGAYIVAPALAFCNSYGTGLTDWNLSSTYGKLGLFLFASLVGNNGGVIAGLAACGVMMSI 480

Query: 380 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 439
           VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK
Sbjct: 481 VSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCVIAPLTFWLFWSAFDIGLPDSPYK 540

Query: 440 APYAVIFREMAILGVEGFSELPKHCLAMCCGFFVAAFLVNLLRDVVPKKIAQFIPIPMAM 499
           APYAVIFREMAILGV+GFSELPKHCLAMC GFFV AFLVN LRD+VPKKIAQFIPIPMAM
Sbjct: 541 APYAVIFREMAILGVQGFSELPKHCLAMCFGFFVTAFLVNFLRDIVPKKIAQFIPIPMAM 600

Query: 500 AVPFYIGAYFAIDMFVGTVILFIWERINRKDAEDYAGAVASGLICGDGIWTIPSAVLSIF 559
           AVPFYIGAYFAIDMFVGTVILFIWERINR+DAEDYAGAVASGLICGDGIWTIPSAVLSIF
Sbjct: 601 AVPFYIGAYFAIDMFVGTVILFIWERINREDAEDYAGAVASGLICGDGIWTIPSAVLSIF 660

Query: 560 RINPPICMSFNPT 572
           RINPPICM+F PT
Sbjct: 661 RINPPICMTFKPT 673

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6R3K63.0e-28383.63Probable metal-nicotianamine transporter YSL6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q6R3K82.3e-27279.96Probable metal-nicotianamine transporter YSL4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q7XRV21.1e-26978.52Probable metal-nicotianamine transporter YSL6 OS=Oryza sativa subsp. japonica OX... [more]
Q7XRV16.7e-23566.85Probable metal-nicotianamine transporter YSL5 OS=Oryza sativa subsp. japonica OX... [more]
Q5JQD74.8e-20160.76Probable metal-nicotianamine transporter YSL12 OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
XP_004148009.10.0100.00probable metal-nicotianamine transporter YSL6 isoform X1 [Cucumis sativus] >KGN5... [more]
XP_008450132.10.099.10PREDICTED: probable metal-nicotianamine transporter YSL6 [Cucumis melo][more]
KAA0060249.10.098.92putative metal-nicotianamine transporter YSL6 [Cucumis melo var. makuwa] >TYJ976... [more]
XP_038894292.10.097.83probable metal-nicotianamine transporter YSL6 [Benincasa hispida][more]
KAG6588889.10.095.48putative metal-nicotianamine transporter YSL6, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
A0A0A0LDJ60.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G565320 PE=4 SV=1[more]
A0A1S3BNL10.099.10probable metal-nicotianamine transporter YSL6 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5D3BE810.098.92Putative metal-nicotianamine transporter YSL6 OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1JPT20.095.30probable metal-nicotianamine transporter YSL6 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1EK770.095.12probable metal-nicotianamine transporter YSL6 OS=Cucurbita moschata OX=3662 GN=L... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004813Oligopeptide transporter, OPT superfamilyTIGRFAMTIGR00728TIGR00728coord: 8..246
e-value: 3.0E-52
score: 176.0
IPR004813Oligopeptide transporter, OPT superfamilyPFAMPF03169OPTcoord: 2..250
e-value: 5.4E-58
score: 197.1
NoneNo IPR availablePANTHERPTHR31645:SF63METAL-NICOTIANAMINE TRANSPORTER YSL4-RELATEDcoord: 1..262
IPR045035Metal-nicotianamine transporter YSL-likePANTHERPTHR31645OLIGOPEPTIDE TRANSPORTER YGL114W-RELATEDcoord: 1..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G15452.T2Cucsat.G15452.T2mRNA
Cucsat.G15452.T1Cucsat.G15452.T1mRNA
Cucsat.G15452.T8Cucsat.G15452.T8mRNA
Cucsat.G15452.T4Cucsat.G15452.T4mRNA
Cucsat.G15452.T3Cucsat.G15452.T3mRNA
Cucsat.G15452.T7Cucsat.G15452.T7mRNA
Cucsat.G15452.T6Cucsat.G15452.T6mRNA
Cucsat.G15452.T5Cucsat.G15452.T5mRNA
Cucsat.G15452.T9Cucsat.G15452.T9mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035672 oligopeptide transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0035673 oligopeptide transmembrane transporter activity