Cucsat.G15325 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G15325
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Auxin-responsive GH3 family protein
Location	ctg2009: 1061320 .. 1064905 (-)
RNA-Seq Expression	Cucsat.G15325
Synteny	Cucsat.G15325

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGACAAGAGTGGCATATGGGAAACAAGTTACTGAAAATTGCAATGTGTGATCTCACAACCCACAATACGATAAAATTTATAGTACTACAGTGTTATGAAAAAGATCTCCAACATTATCTTTCATTCATTAATTGTCATGAAAATAATATCTAAGAAATTATAGAGTCTATAAAATAGCTAAATAATAAATTAATAGATCATTTGATGACATCTTAAGTAATGGATGAGGAGCATGCTTAGTATTAGAAAATTTGCATCCAAACAATTACTTTTTCATTAATAATTAACTATGTCCTTATTTTGCATAACAAATGATTTAGGTCAAAAGCTTAACTATGTCCTTAGTTTGCATAACAGTTTATTTAGGTCAAAGTAAAGCTTTTGACCCAAGTATTATTTTTCATTCAAGATAATGATTTAACTTTTAAAATTTAGTATTATAATCATTTAATTTTTTTAAAGTTAAGTATATAATCATTTGAAGATAGTTTTAGTCATGTGTTTGAAATTGAGAGAATTTTATCTAAAATATAACGATATATATTTGAATGATTGTTTAGATAAGAAAGATGAGATTTTGAAAAGTGTAAACATGACTATATAAATTAAAAATGAGATGGTAAGTCGGAAAATAAGAAAAGATAGTTTAAATTGAAAACTATTATAAAAAATGAGTTTTTTTTTTAAAAAAAGTATTACAAATAGAGTTTAAAAGGTTTAAATCTAAACATGAATTTTAGATTATATTAGGATACAAATTTATTTGACTGAGAATGATGGAATATGTTAAAAAATACATAGGGTGGATGGATGGAATATGTTAAAAAATACATAGGATGGATGGATGGAGTAGAATAGGCAGTGATATGATTAGAAGTAGTAGTTAAAGTAACGAATTGAATTATGGGTTTAAAGTTGTTGTATAATTAAGTGTATTAGAGGCCAAACTTAGAAGCTAAAATGTGTTTTAGTAAGTGAAAATGCAAAATCAAGAATGGAGGGAAAAGAAAAGACCTAACCTTCACATGCTCATCTCACTTGCTGCAATGTCTACGTGTTGTGGGTCATCCTTTGTCTCCATTTCCCCCTACATTTCCTTTCCACAACACAGAATTCCTCTCTCTTCTCCCCCCCTCTCTCTCTCTTTACGCTCCGTATAATTCCGTAAGCTTCCTCCCAACTCCCTCTCTCTCTCTCCCTCTCTCTTCTTCATTTCTTTTTAAATATTTAAGTAAAACAACTCTTACCCCACCCAGTCAACCTTCCTACGTGGCACTTCTCCCCCACTATATATATCCTTCCCTTCTCCCCTTTCCTCCACCGCCCCCCCCCTTCTTACTCATCACCCTCCTTCCTCTTCTTCATCTTCTTCCTCCCCTTCTTTCAATGGCTGTCCATTCTTCTCTCTCTTCACCTCTCGGCCCTCCTGCCTGTGAAAGAGACTCTAAGCCTCTTCAATTCATCGATGAAATGACTCGTAACGCCCACGCCGTCCAACACACCCTCCTCTCTGAAATCTTGTCCAGAAACGCCTCTACCGAGTACCTCCGCCGTTACTCCCTCGACGGCGCTACCGATCCTCAAACTTTCAAAGCTAAACTCCCTGTCATCACCTACGAGGATCTCCAACCTGAAATACAACGAATTGCCAGTGGAGATCGCTCTCCTATTCTCTCCTCTCATCCCATCTCCGAGTTCCTCACTAGGTAATTGAATGAAACGTAACACTCTTGCTCATCAGAAATATTGAATTTAACAAACATAATTCGTTTATTACCTGAATTGAATCCAGTGACTGATGGGGTGATTTTTGCAGTTCTGGTACTTCCGCCGGAGAGAGAAAACTTATGCCAACGATTAAGGAGGAACTGGATCGTCGTCAACTTCTATACAGTCTACTCATGCCAGTTATGAACCTGTAAGAGAAAGCAACAACAATATGAACATTTTGAATTTATTATTAAAATTAAAAGCGCGTAAAATTAAATTAAATCAAATTAAATTTGACAGCTATGTACCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCTCGACCTGTTTTAACCAGTTACTACAAGAGTGACCATTTCAAGACCCGACCCTACGACCCCTTTATGGTTTACACCAGCCCCAATGAAGCCATCCTTTGCCCCGATTCCTTCCAAAGCATGTACACTCAGATGCTCTGTGGTCTTCTCCAACGCAACCAAGTCCTCCGCCTCGGCGCCGTCTTTGCCTCTGGTTTACTCCGTGCCATCCGCTTTCTTCAATTGAACTGGCACGATCTCGCTACTGATATTCGTAACGGAACCTTGAATTCCAGAATCACCGATCCCTCCTTAAGAGATTGCATCCAGAAGATCCTGAAACCCGACGCCCAACTGGCGGATTTCGTTTCAGATGAATGTTCGAAGGAAGAGTGGGAGGGAATTGTAACCAGGATTTGGCCGAAAACGAAATACCTCGACGTAATCGTCACCGGAGCTATGGCCCAGTACATTCCAACCCTCGATTTCTACAGCGGCGGATTACCTCTGGCTTGCACCATGTACGCATCATCCGAGTGTTATTTCGGACTGAATCTAAACCCTATGTGTAAACCATCGGAAGTTTCATACACTATAATGCCAAACATGGCCTACTTCGAGTTCCTCCCTCTGGAGCAGAATTCCGGAAGCGATTCATCCCCAAAACTCGTGGACCTGGTGGATGTAGAGATGGGAAAAGAATACGAACTGGTAATCACAACATACGCAGGATTGTATCGTTACCGGGTGGGGGATATACTCCGGGTGACAGGATTCCACAACGCGGCACCGCAGTTCCACTTCGTGAGGAGAAAGAACGTAGTGCTGAGTATAGATTCGGACAAAACAGACGAAGCAGAGTTGCAGAAGGCAGTGGAGAAGGCGTCAGAGGTGTTAAAGCAATGGAAAGCGAGCGTGGTGGAGTACACGAGTTACGCAGAGACGAAGACGATTCCGGGGCATTACGTGATATATTGGGAATTGATGGTGAAAGAAGGGGGGGAGGAAGCAGGGAAATGGGGGAGGAAGGAAGTGATGGAAGAGTGTTGTTTAAGAATGGAGGAATCGATGAACTCGGTGTACAGGCAAGGGAGAGTGGCGGACAATTCAATAGGAGCATTGGAAATAAGAGTGGTGAAGAGTGGAACGTTTGAAGAATTGATGGATTATGCGATATCGAGAGGAGCGTCAATCAACCAATACAAGGTTCCAAGGTGTGTGAACTTTACACCTATTATGGAACTCCTGGATTCTAGAGTAGTATCCACGCATTTTAGTCCTGCTCTCCCACATTGGACTCCAACTACAAAATCCTAATTTTATTATTATTTTTTTTTCAATTTTCTCTTTCTCAAATTTGGGGGAAAAAAAGAAAAAAAATGTAGGTTCTAATACTCTGTATTCTACTTCTATTGTATTCTATCAAATCTTGTTCTCATAACTAAATTTATGCAAAAACAAAATCACATTTCTTGTCCAAATCTTTCACTTTTACCTCTCAAAAACACATCAT

Coding sequence (CDS)

ATGGCTGTCCATTCTTCTCTCTCTTCACCTCTCGGCCCTCCTGCCTGTGAAAGAGACTCTAAGCCTCTTCAATTCATCGATGAAATGACTCGTAACGCCCACGCCGTCCAACACACCCTCCTCTCTGAAATCTTGTCCAGAAACGCCTCTACCGAGTACCTCCGCCGTTACTCCCTCGACGGCGCTACCGATCCTCAAACTTTCAAAGCTAAACTCCCTGTCATCACCTACGAGGATCTCCAACCTGAAATACAACGAATTGCCAGTGGAGATCGCTCTCCTATTCTCTCCTCTCATCCCATCTCCGAGTTCCTCACTAGTTCTGGTACTTCCGCCGGAGAGAGAAAACTTATGCCAACGATTAAGGAGGAACTGGATCGTCGTCAACTTCTATACAGTCTACTCATGCCAGTTATGAACCTCTATGTACCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCTCGACCTGTTTTAACCAGTTACTACAAGAGTGACCATTTCAAGACCCGACCCTACGACCCCTTTATGGTTTACACCAGCCCCAATGAAGCCATCCTTTGCCCCGATTCCTTCCAAAGCATGTACACTCAGATGCTCTGTGGTCTTCTCCAACGCAACCAAGTCCTCCGCCTCGGCGCCGTCTTTGCCTCTGGTTTACTCCGTGCCATCCGCTTTCTTCAATTGAACTGGCACGATCTCGCTACTGATATTCGTAACGGAACCTTGAATTCCAGAATCACCGATCCCTCCTTAAGAGATTGCATCCAGAAGATCCTGAAACCCGACGCCCAACTGGCGGATTTCGTTTCAGATGAATGTTCGAAGGAAGAGTGGGAGGGAATTGTAACCAGGATTTGGCCGAAAACGAAATACCTCGACGTAATCGTCACCGGAGCTATGGCCCAGTACATTCCAACCCTCGATTTCTACAGCGGCGGATTACCTCTGGCTTGCACCATGTACGCATCATCCGAGTGTTATTTCGGACTGAATCTAAACCCTATGTGTAAACCATCGGAAGTTTCATACACTATAATGCCAAACATGGCCTACTTCGAGTTCCTCCCTCTGGAGCAGAATTCCGGAAGCGATTCATCCCCAAAACTCGTGGACCTGGTGGATGTAGAGATGGGAAAAGAATACGAACTGGTAATCACAACATACGCAGGATTGTATCGTTACCGGGTGGGGGATATACTCCGGGTGACAGGATTCCACAACGCGGCACCGCAGTTCCACTTCGTGAGGAGAAAGAACGTAGTGCTGAGTATAGATTCGGACAAAACAGACGAAGCAGAGTTGCAGAAGGCAGTGGAGAAGGCGTCAGAGGTGTTAAAGCAATGGAAAGCGAGCGTGGTGGAGTACACGAGTTACGCAGAGACGAAGACGATTCCGGGGCATTACGTGATATATTGGGAATTGATGGTGAAAGAAGGGGGGGAGGAAGCAGGGAAATGGGGGAGGAAGGAAGTGATGGAAGAGTGTTGTTTAAGAATGGAGGAATCGATGAACTCGGTGTACAGGCAAGGGAGAGTGGCGGACAATTCAATAGGAGCATTGGAAATAAGAGTGGTGAAGAGTGGAACGTTTGAAGAATTGATGGATTATGCGATATCGAGAGGAGCGTCAATCAACCAATACAAGGTTCCAAGGTGTGTGAACTTTACACCTATTATGGAACTCCTGGATTCTAGAGTAGTATCCACGCATTTTAGTCCTGCTCTCCCACATTGGACTCCAACTACAAAATCCTAA

Protein sequence

MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLDGATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRFLQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRIWPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIPGHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS

Homology

[Close]

BLAST of Cucsat.G15325 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 8.1e-276
Identity = 468/596 (78.52%), Postives = 521/596 (87.42%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAV S+LSSPLGPPACE+D+K L+FI+EMTRNA  VQ  LL+EIL+RNA TEYLRR++L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD  TFK K+PVITYEDLQPEIQRIA GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HF++RPYDP+ VYTSPNEAILCPDSFQSMYTQMLCGLL R  VLR+GAVFASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQL+W   A DI  G L+S ITDPS+R C+  ILKPD  LA+F+  EC  + WE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+PL          K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVL-KQWKASVVEYTSYAETKTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVE AS +L ++  + V EYTSYA+T TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRV 540
           PGHYV+YWEL+V++G  +       E +  CCL MEES+NSVYRQ RVADNS+G LEIRV
Sbjct: 481 PGHYVLYWELLVRDGARQPS----HETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRV 540

Query: 541 VKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP 596
           V++GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVS HFSP+LPHWTP
Sbjct: 541 VRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTP 585

[Close]

BLAST of Cucsat.G15325 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 889.4 bits (2297), Expect = 2.2e-257
Identity = 441/596 (73.99%), Postives = 496/596 (83.22%), Query Frame = 0


Query: 5   SSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLDGATD 64
           ++L +P      E D + L+FIDEMT N  AVQ  +L EIL RNA TEYL +  LDGATD
Sbjct: 12  TALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATD 71

Query: 65  PQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEE 124
              F+AK+PV++Y+DLQP IQRIA+GDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +E
Sbjct: 72  RAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDE 131

Query: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKT 184
           LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKSDHFK 
Sbjct: 132 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKN 191

Query: 185 RPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRFLQLN 244
           RPYDP+  YTSP  AILC D+FQSMY QM+CGL QRN VLRLGAVFASGLLRAIRFLQLN
Sbjct: 192 RPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLN 251

Query: 245 WHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRIWPKT 304
           W  LA DI +G L  R+TDPS+R+ +  IL PD +LA  +  ECSK +W GI+TR+WP T
Sbjct: 252 WEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNT 311

Query: 305 KYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNM 364
           KYLDVIVTGAMAQYIPTL+FYSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM
Sbjct: 312 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 371

Query: 365 AYFEFLPL-EQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGFHNA 424
            YFEFLP+ E  + S  + +LVDL  VE+G+EYELVITTYAGL RYRVGD+LRVTGFHNA
Sbjct: 372 GYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNA 431

Query: 425 APQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIPGHY 484
           APQF FVRRKNV+LSI+SDKTDEAELQ+AVE+AS +L+   ASVVEYTS A TK IPGHY
Sbjct: 432 APQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHY 491

Query: 485 VIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVVKSG 544
           VIYWEL+ K  G         + +  CCL MEE++N+VYRQ RVAD SIG LEIRVV+ G
Sbjct: 492 VIYWELLTKGAGATVVD---ADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPG 551

Query: 545 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS 600
           TFEELMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALPHWTP  +S
Sbjct: 552 TFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARRS 604

[Close]

BLAST of Cucsat.G15325 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 2.2e-257
Identity = 441/596 (73.99%), Postives = 496/596 (83.22%), Query Frame = 0


Query: 5   SSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLDGATD 64
           ++L +P      E D + L+FIDEMT N  AVQ  +L EIL RNA TEYL +  LDGATD
Sbjct: 12  TALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATD 71

Query: 65  PQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEE 124
              F+AK+PV++Y+DLQP IQRIA+GDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +E
Sbjct: 72  RAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDE 131

Query: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKT 184
           LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKSDHFK 
Sbjct: 132 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKN 191

Query: 185 RPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRFLQLN 244
           RPYDP+  YTSP  AILC D+FQSMY QM+CGL QRN VLRLGAVFASGLLRAIRFLQLN
Sbjct: 192 RPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLN 251

Query: 245 WHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRIWPKT 304
           W  LA DI +G L  R+TDPS+R+ +  IL PD +LA  +  ECSK +W GI+TR+WP T
Sbjct: 252 WEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNT 311

Query: 305 KYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNM 364
           KYLDVIVTGAMAQYIPTL+FYSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM
Sbjct: 312 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 371

Query: 365 AYFEFLPL-EQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGFHNA 424
            YFEFLP+ E  + S  + +LVDL  VE+G+EYELVITTYAGL RYRVGD+LRVTGFHNA
Sbjct: 372 GYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNA 431

Query: 425 APQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIPGHY 484
           APQF FVRRKNV+LSI+SDKTDEAELQ+AVE+AS +L+   ASVVEYTS A TK IPGHY
Sbjct: 432 APQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHY 491

Query: 485 VIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVVKSG 544
           VIYWEL+ K  G         + +  CCL MEE++N+VYRQ RVAD SIG LEIRVV+ G
Sbjct: 492 VIYWELLTKGAGATVVD---ADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPG 551

Query: 545 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS 600
           TFEELMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALPHWTP  +S
Sbjct: 552 TFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARRS 604

[Close]

BLAST of Cucsat.G15325 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 3.6e-255
Identity = 443/597 (74.20%), Postives = 507/597 (84.92%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           M V S+L SP+      +D K L+FI+EMTRN   VQ  ++ EILSRN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD +TFK K+PV+ Y+DL+PEIQRIA+GDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKS+
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
            FK RP DP+ VYTSPNEAILCPDS QSMYTQMLCGLL R++VLRLGAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKIL-KPDAQLADFVSDECSKE-EWEGIVT 300
           LQ NW +LA DI  GTL+SRI+DP++++ + KIL KPD +LADF++  C ++  WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L++YSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVT 420
           TIMPNMAYFEFLP  +     S  +LV+L DVE+GKEYELVITTYAGL RYRVGDIL+VT
Sbjct: 361 TIMPNMAYFEFLPHHEVPTEKS--ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVT 420

Query: 421 GFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKT 480
           GF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVE AS +L +    V+EYTSYAETKT
Sbjct: 421 GFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKT 480

Query: 481 IPGHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIR 540
           IPGHYVIYWEL+VK+           EVM  CCL MEES+NSVYRQ RVAD SIG LEIR
Sbjct: 481 IPGHYVIYWELLVKDQTNPP----NDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP 596
           VVK+GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVSTHFSPALPHW+P
Sbjct: 541 VVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSP 591

[Close]

BLAST of Cucsat.G15325 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 6.9e-251
Identity = 427/595 (71.76%), Postives = 505/595 (84.87%), Query Frame = 0


Query: 15  ACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLDGATDPQTFKAKLPV 74
           ACERD++ L+FI+EMTR   AVQ  +L+ IL+RN   EYLRR+ ++G TD + FKA++PV
Sbjct: 25  ACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPV 84

Query: 75  ITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSL 134
           +TYEDL+PEI+RIA+GDRS I+SSHPI+EFLTSSGTSAGERKLMPTI++ELDRRQ+LYSL
Sbjct: 85  VTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSL 144

Query: 135 LMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSDHFKTRPYDPFMVYT 194
           LMPVMNLYVPGLDKGKGLYFLF+KSET+TPGGL ARPVLTSYYKSDHFK RP+DP+ VYT
Sbjct: 145 LMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYT 204

Query: 195 SPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRFLQLNWHDLATDIRN 254
           SP  AILC D+FQSMY QMLCGL+ R +VLR+GAVFASGLLRAIRFLQL+W +LA DIR 
Sbjct: 205 SPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRT 264

Query: 255 GTLNSRITDPSLRDCIQKIL-KPDAQLADFVSDECSKEEWEGIVTRIWPKTKYLDVIVTG 314
           GTL++++T+PS+RD + ++L  PDA+LA FV  EC K++WEGI+TR+WP TKYLDVIVTG
Sbjct: 265 GTLSAKVTEPSIRDAVAEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVTG 324

Query: 315 AMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPLE 374
           AMAQYIPTL FYSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM YFE +P +
Sbjct: 325 AMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPHD 384

Query: 375 QNS----GSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHF 434
            ++         P+LVDL D E+G+EYELVITTYAGL RYRVGDIL+VTGFHNAAPQF F
Sbjct: 385 PDAPPLPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQFRF 444

Query: 435 VRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIPGHYVIYWEL 494
           VRRKNV+LSIDSDKTDEAELQ AVE+AS +L  + AS+VEYTS A+  TIPGHYV+YWEL
Sbjct: 445 VRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVYWEL 504

Query: 495 MVKEGG------EEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVVKSG 554
           MV+EGG      EE G    + V E CCL MEE++N+VYRQGR  + +IG LEIRVV++G
Sbjct: 505 MVREGGAWPPPAEEEG----RGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAG 564

Query: 555 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTK 599
           TFEE+MDYAISRGASINQYK PRCV+F PI+ELL+SRV+S HFSPA P ++P  K
Sbjct: 565 TFEEVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSPHKK 614

[Close]

BLAST of Cucsat.G15325 vs. NCBI nr
Match: XP_004140723.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN57485.1 hypothetical protein Csa_011380 [Cucumis sativus])

HSP 1 Score: 1203 bits (3113), Expect = 0.0
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540
           GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV
Sbjct: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS 599
           KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS
Sbjct: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS 599

[Close]

BLAST of Cucsat.G15325 vs. NCBI nr
Match: XP_008465614.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >KAA0037204.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1156 bits (2991), Expect = 0.0
Identity = 576/600 (96.00%), Postives = 587/600 (97.83%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACE D+KPLQFIDEMTRNA AVQHT+L+EILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNWHDLATDIRNGTLNSRITDPSLRDCI KILKPD+QLADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPLE NSGSDSSPKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE ASE+LK+WKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGK--WGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIR 540
           GHYVIYWELMVKE G++ GK  W  KEVMEECCLRMEESMNSVYRQGRVAD+SIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTK 598
           VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP  K
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPARK 600

[Close]

BLAST of Cucsat.G15325 vs. NCBI nr
Match: XP_038877583.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida])

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 565/595 (94.96%), Postives = 581/595 (97.65%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACE D+KPLQFID+MTRNA AVQHT+L+EILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACENDAKPLQFIDQMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNW DLATDIRNGTLNSRITDPSLRDCI+KILKPDA+LADF+SDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLATDIRNGTLNSRITDPSLRDCIEKILKPDAELADFLSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP E NSG+DS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGNDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASE+LK+WK SVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASELLKEWKTSVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540
           GHYVIYWELMVKE  EE GKW  KEVME+CCL MEES+NSVYRQGRVADNSIGALEIRVV
Sbjct: 481 GHYVIYWELMVKEATEE-GKWPSKEVMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP 595
           KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALPHWTP
Sbjct: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTP 594

[Close]

BLAST of Cucsat.G15325 vs. NCBI nr
Match: XP_022922958.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 546/595 (91.76%), Postives = 572/595 (96.13%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE+D+K LQFI++MTRNA AVQ T+L+EIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNW DLA DIRNGTLNS+ITDPSLRDCI+KILKPDA+LADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP E N+ SDS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE AS++LK+WKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540
           GHYVIYWELMVKE     GKW  KEVMEECCL MEES+NSVYRQGRVAD+SIGALEIRVV
Sbjct: 481 GHYVIYWELMVKEA---EGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP 595
           K+GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALP WTP
Sbjct: 541 KNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTP 592

[Close]

BLAST of Cucsat.G15325 vs. NCBI nr
Match: KAG6576928.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014954.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1100 bits (2844), Expect = 0.0
Identity = 544/595 (91.43%), Postives = 572/595 (96.13%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE+D+K LQFI++MTRNA AVQ T+L+EIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGA+FASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAIFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNW DLA DIRNGTLNS+ITDPSLRDCI+KILKPDA+LADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP E N+ SDS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE AS++LK+WKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540
           GHYVIYWELMVKE     GKW  KEVME+CCL MEES+NSVYRQGRVAD+SIGALEIRVV
Sbjct: 481 GHYVIYWELMVKEA---EGKWPSKEVMEDCCLAMEESLNSVYRQGRVADHSIGALEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP 595
           K+GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALP WTP
Sbjct: 541 KNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTP 592

[Close]

BLAST of Cucsat.G15325 vs. ExPASy TrEMBL
Match: A0A0A0L698 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G198490 PE=3 SV=1)

HSP 1 Score: 1203 bits (3113), Expect = 0.0
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540
           GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV
Sbjct: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS 599
           KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS
Sbjct: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTKS 599

[Close]

BLAST of Cucsat.G15325 vs. ExPASy TrEMBL
Match: A0A5A7T148 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold379G001170 PE=3 SV=1)

HSP 1 Score: 1156 bits (2991), Expect = 0.0
Identity = 576/600 (96.00%), Postives = 587/600 (97.83%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACE D+KPLQFIDEMTRNA AVQHT+L+EILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNWHDLATDIRNGTLNSRITDPSLRDCI KILKPD+QLADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPLE NSGSDSSPKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE ASE+LK+WKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGK--WGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIR 540
           GHYVIYWELMVKE G++ GK  W  KEVMEECCLRMEESMNSVYRQGRVAD+SIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTK 598
           VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP  K
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPARK 600

[Close]

BLAST of Cucsat.G15325 vs. ExPASy TrEMBL
Match: A0A1S3CP72 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103503255 PE=3 SV=1)

HSP 1 Score: 1156 bits (2991), Expect = 0.0
Identity = 576/600 (96.00%), Postives = 587/600 (97.83%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAVHSSLSSPLGPPACE D+KPLQFIDEMTRNA AVQHT+L+EILSRNASTEYLRRYSLD
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNWHDLATDIRNGTLNSRITDPSLRDCI KILKPD+QLADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPLE NSGSDSSPKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE ASE+LK+WKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGK--WGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIR 540
           GHYVIYWELMVKE G++ GK  W  KEVMEECCLRMEESMNSVYRQGRVAD+SIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPTTK 598
           VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP  K
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPARK 600

[Close]

BLAST of Cucsat.G15325 vs. ExPASy TrEMBL
Match: A0A6J1E8A0 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111430783 PE=3 SV=1)

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 546/595 (91.76%), Postives = 572/595 (96.13%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE+D+K LQFI++MTRNA AVQ T+L+EIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNW DLA DIRNGTLNS+ITDPSLRDCI+KILKPDA+LADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP E N+ SDS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE AS++LK+WKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540
           GHYVIYWELMVKE     GKW  KEVMEECCL MEES+NSVYRQGRVAD+SIGALEIRVV
Sbjct: 481 GHYVIYWELMVKEA---EGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP 595
           K+GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALP WTP
Sbjct: 541 KNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTP 592

[Close]

BLAST of Cucsat.G15325 vs. ExPASy TrEMBL
Match: A0A6J1J7V1 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111483372 PE=3 SV=1)

HSP 1 Score: 1098 bits (2841), Expect = 0.0
Identity = 543/595 (91.26%), Postives = 571/595 (95.97%), Query Frame = 0


Query: 1   MAVHSSLSSPLGPPACERDSKPLQFIDEMTRNAHAVQHTLLSEILSRNASTEYLRRYSLD 60
           MAV SSLSSPLGPPACE+D+K LQFI++MTRNA AVQ T+L+EIL+RNA+TEYLRRYSLD
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TDP TFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GTTDPHTFKGKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPF+VYTSPNEAILCPDSFQSMYTQMLCGLLQR+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCIQKILKPDAQLADFVSDECSKEEWEGIVTRI 300
           LQLNW DLA DIRNGTLN++ITDPSLRDCI+KILKPDA+LADFVSDECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPLEQNSGSDSSPKLVDLVDVEMGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP E N+ SDS PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVEKASEVLKQWKASVVEYTSYAETKTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVE AS++LK+WKASVVEYTSYAETKTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKEGGEEAGKWGRKEVMEECCLRMEESMNSVYRQGRVADNSIGALEIRVV 540
           GHYVIYWELMVKE     GKW  KEVMEECCL MEES+NSVYRQGRVAD+SIGALEIRVV
Sbjct: 481 GHYVIYWELMVKEA---EGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTP 595
           K+GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRV+S HFSPALP WTP
Sbjct: 541 KNGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVISAHFSPALPRWTP 592

The following BLAST results are available for this feature:

BLAST of Cucsat.G15325 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82333	8.1e-276	78.52	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]
A3BLS0	2.2e-257	73.99	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399...	[more]
Q0D4Z6	2.2e-257	73.99	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3...	[more]
O22190	3.6e-255	74.20	Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
P0C0M2	6.9e-251	71.76	Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo...	[more]

BLAST of Cucsat.G15325 vs. NCBI nr


Match Name	Identity	Description
XP_004140723.1	100.00	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN57485...	[more]
XP_008465614.1	96.00	PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >...	[more]
XP_038877583.1	94.96	probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida]	[more]
XP_022922958.1	91.76	probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata]	[more]
KAG6576928.1	91.43	putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma ...	[more]

BLAST of Cucsat.G15325 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A0A0L698	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G198490 PE=3 SV=1	[more]
A0A5A7T148	96.00	Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa...	[more]
A0A1S3CP72	96.00	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1E8A0	91.76	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36...	[more]
A0A6J1J7V1	91.26	probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 329..349
None	No IPR available	PANTHER	PTHR31901:SF7	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATED	coord: 22..480
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 25..453 e-value: 1.4E-161 score: 539.0
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 22..480

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G15325.T1	Cucsat.G15325.T1	mRNA
Cucsat.G15325.T2	Cucsat.G15325.T2	mRNA
Cucsat.G15325.T3	Cucsat.G15325.T3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016881	acid-amino acid ligase activity