Cucsat.G14872 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G14872
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Aspergillus nuclease S(1)
Location	ctg1869: 6523380 .. 6526005 (+)
RNA-Seq Expression	Cucsat.G14872
Synteny	Cucsat.G14872

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCATGTATTTCATGCACATTAAAAGAATTCCCCAACTTGCTTGGCGTTCAACTCTAAGCAAGTTTCAGATTATGGTATCTTAAATATGTGCCAATATTTCAATACTAATAACTTGTACTCAACCATCACCTCTCTTTCTATATATATATATATATAGATACATATAGACTTGTGCAAACATTTCTTTTCAACTCAACAAAATGGGAAAATTGGTATTTTTGGTGGTGTTAATTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGTAACTTTATTTACTTTTTCTTGTCTGATGATCTTTTAATTAAATTGGACGTTTTGGATCAAATTCTGCCTTGACCATTTGAACTCAACACATAGGGTCATTTTTACTCTTAAATTTGACAAACTGTCTCCATTTTTATCTGCAAATTTCTATTTTCTTTAATCAGTTTTTATATAAGCTTAAAAAAAATTGTTATATGGGTGGGATCTGAGGAGTTTGTTATGAAATCAACAAATAAAACAAGCATAAAAACGTAAATGAGGAGAGAATCGACATAGAAGCATATTAGCTACATTTATGCTCATTAGAAAGAATATTTCAGATTACAGAACAAAGACGCTTCTATAAATGGGGAGTTCATACAACCAATTTGATCTTCAAATCTTAAGATTAAAATGGTAAACAACGTAAATACAGTGAGATCTTAAACATTTTTGTTTAATTAGTTGTTGAGAGGTGAGATATCAGATCTAAATGTATTCACCTCAGTTATAAAATATATATCAGGCAAGAAACTGATGTGTTTTGAAAGAGAAATTAACAACAAAACAAATTTTGGGCTGAATTTTGGAAGATTTATCAGACAATTGACATTGTAAATTGATGTAGCTCAATTCGATCATGTCAAACTTTTTTTTTGTTGAAAGCAGCGCGAGTCTTAAAAAGGAGGATTTTACATGTTAAACAAATTTAGAAACAAAAATTTTAAAATTTGAGACACACAATTGAATATTGAAGAGGACAACTAATGGGTAAGTTTAGATTATAATCCAACCACATTCAGGAGCTACTGATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATCTATCGGCTATGTGCGTATGGCCGGACCAAATCCGACTCCAGTCTAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGTAAAGTAACACCCTTTTATTCTCCCATCCCTAAGGCTTTCGAATTATTAAACTAAACGGTGCGTTTTTGTTTAGGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACCGAACACAAGGCTTCGACAGTCCTCGTAACTGACCCGACCCGACCCACTCGAATTATTCCTGTGATAATATGAAAATTATTTAATTTCATTTTTATTATTCTAGATAACTTGACCGAGGCCTTGCTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGGTAATACTAAAGTTATTCAAAAACGTTTATGTGTTGAATTGCTTTAATTTAATTATGAGATATGTAATTATAACTTATTTTACATATACATATATATACCTATAATTTCAGCCATTGCATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACCATAGAAGTACGATGGTTCCGTCGTAAATCAAACCTCCATCACGTAAGATCTTAATCTAAAAACATACCAAAACAATATGAATCTATATGTTTTAAGTTTTATGAATTCTATTACATTTAGATTATAAATTACTAATTATATGGAATAATGCAGGTGTGGGATAGAGACATTATTCTTGAAGCTCTAGGAGATTATTACGACAAAGACGGTGGCCTTCTCTTAGATGAACTTAACCGGAATTTAACTCAGGTAATTAATTAATACTTTTTATTTTTTCTTTATTACATTAGTACATTAGTTTATAATGTTAGGATATTAGAAGGTTTACTATCTTCTTATCAAAATTCTGTTTTTGTAGGGAATTTGGAGCAATGACGTTTCGGAATGGGAGCGTTGTTCTACTGTTAATTCATGTGTTAATAGGTATTTATAGAAAATGAAATTAGGTTATATTTTGTAAATATTTTTTTGTTTTTTTTTTAACCATAGTAACAAACTTGTGGATTAGGTGGGCTGATGAGAGTACAGGGTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGGTAAACCCAATCAATCAATCAATCAAACATTGTTTAAATTAATATCATAGTCATTAATTCCTAAAATTATTTGAAATATAACATATGATGAGAGGATAATGAAATCCGTAACTGATGTGATTTGTGTACAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACGAGGATTTGCCTATTCATCTTGATTAATTCCACCATCAAATTTAGGATTTCTTTTTCTTCTTTCTTCTAACTTAATTATGTCAGGAAAATATCTTAGATTTCAAAGGTTACTAGAAATGAAAGCAAATTGTAGTGTTGAATAAATTGGCATTTTAGTTCATTTTACAAAATTTCATCAACAAATTCACATGCTCTTTACATAGATGTAAATGAATTAATCAAAGTAATAATGCAGTACATCTAAATTGGTATACTACATGATTTGTAACAAATAACATTTGATCAAATGTAACCCCTATATATCATAAATGCAC

Coding sequence (CDS)

ATGGGAAAATTGGTATTTTTGGTGGTGTTAATTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGAGCTACTGATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATCTATCGGCTATGTGCGTATGGCCGGACCAAATCCGACTCCAGTCTAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGTAAAGTAACACCCTTTTATTCTCCCATCCCTAAGGCTTTCGAATTATTAAACTAA

Protein sequence

MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKSKVTPFYSPIPKAFELLN

Homology

[Close]

BLAST of Cucsat.G14872 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 1.6e-69
Identity = 116/196 (59.18%), Postives = 150/196 (76.53%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQL 60
           +CVWPDQIR   KYRW S LHY +TPD +CS+ Y RDCH+  G  DMCV GAI+NFT+QL
Sbjct: 68  LCVWPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQL 127

Query: 61  TTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVW 120
             Y     D  +N+TEALLFLSHF+GDIHQP+HVGF SD GGNTI++RW++ KSNLHHVW
Sbjct: 128 QHYGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVW 187

Query: 121 DRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGL 180
           DR+IIL AL + YDK+  LL ++L +N+T G+W +D+S W  C+ + +C +++A ES  L
Sbjct: 188 DREIILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKL 247

Query: 181 ACKWAYEGVEAGITLS 196
           ACKW Y+GV++G TLS
Sbjct: 248 ACKWGYKGVKSGETLS 263

[Close]

BLAST of Cucsat.G14872 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 8.3e-58
Identity = 100/195 (51.28%), Postives = 136/195 (69.74%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60
           +C+W D+++   +Y W+SPLHY NTPD+CS+ Y RDC +++G+   CVAGAI N+TTQL 
Sbjct: 67  LCLWADRVKF--RYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLL 126

Query: 61  TYRT-QGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVW 120
           +Y+T     S +NLTEALLF+SHF+GDIHQPLHV + SD GGNTIEV W+ RK+NLHH+W
Sbjct: 127 SYKTAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIW 186

Query: 121 DRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGL 180
           D +II  A  D Y+     ++D L +N+T   W++ V  WE C+   +C + +A E    
Sbjct: 187 DSNIIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQA 246

Query: 181 ACKWAYEGVEAGITL 195
           AC WAY+GV  G TL
Sbjct: 247 ACDWAYKGVTEGDTL 258

[Close]

BLAST of Cucsat.G14872 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.0e-56
Identity = 107/197 (54.31%), Postives = 129/197 (65.48%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQL 60
           +C WPD+I+   ++RW SPLHY +TPD  C++ Y RDCH+     D CV GAI N+T QL
Sbjct: 64  VCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQL 123

Query: 61  TTYRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHV 120
            +         H NLTEAL+FLSHF+GDIHQPLHVGF  D GGNTI VRW+RRK+NLHHV
Sbjct: 124 MSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHV 183

Query: 121 WDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADEST 180
           WD  II  AL  YY+K   L+++ L  NLT   WSNDV  WE C     +C N +A ES 
Sbjct: 184 WDNMIIESALKTYYNKSLPLMIEALQANLTND-WSNDVPLWESCQLNQTACPNPYASESI 243

Query: 181 GLACKWAYEGVEAGITL 195
            LACK+AY     G TL
Sbjct: 244 NLACKYAYRNATPGTTL 259

[Close]

BLAST of Cucsat.G14872 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.3e-50
Identity = 98/196 (50.00%), Postives = 121/196 (61.73%), Query Frame = 0


Query: 2   CVWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 61
           C WPD+I+  S+++W S LHY NTP+  C++ Y RDCH+     D CV GAI N+T QL 
Sbjct: 62  CSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLM 121

Query: 62  TYRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVW 121
           +      +  H NLTEALLFLSH++GD+HQPLH GF  D GGNTI V W+  KSNLHHVW
Sbjct: 122 SASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVW 181

Query: 122 DRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTG 181
           D  II  AL  YY+     ++  L   L  G WSNDV  W+ C     +C N +A ES  
Sbjct: 182 DNMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESID 241

Query: 182 LACKWAYEGVEAGITL 195
           LACK+AY     G TL
Sbjct: 242 LACKYAYRNATPGTTL 256

[Close]

BLAST of Cucsat.G14872 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 3.5e-48
Identity = 96/197 (48.73%), Postives = 121/197 (61.42%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQL 60
           +C WPD+I+   ++RW S LH+A+TPD  C++ Y RDC       D CV GAI N+T QL
Sbjct: 64  VCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQL 123

Query: 61  TTYRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHV 120
            +         H NLTEAL+FLSH++GDIHQPLH GF  D GGN I+V W+ +++NLH V
Sbjct: 124 MSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRV 183

Query: 121 WDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADEST 180
           WD  II  AL  YY+     ++ EL   L  G WSNDV  WE C     +C N +A ES 
Sbjct: 184 WDDMIIESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASESI 243

Query: 181 GLACKWAYEGVEAGITL 195
            LACK+AY    AG TL
Sbjct: 244 DLACKYAYRNATAGTTL 254

[Close]

BLAST of Cucsat.G14872 vs. NCBI nr
Match: NP_001292654.1 (endonuclease 1 precursor [Cucumis sativus] >ACO72982.2 bifunctional nuclease precursor [Cucumis sativus] >KAE8652702.1 hypothetical protein Csa_013303 [Cucumis sativus])

HSP 1 Score: 427 bits (1098), Expect = 1.56e-149
Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60
           MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 64  MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 123

Query: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120
           TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD
Sbjct: 124 TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 183

Query: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180
           RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA
Sbjct: 184 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 243

Query: 181 CKWAYEGVEAGITLS 195
           CKWAYEGVEAGITLS
Sbjct: 244 CKWAYEGVEAGITLS 258

[Close]

BLAST of Cucsat.G14872 vs. NCBI nr
Match: XP_008442043.1 (PREDICTED: endonuclease 1 [Cucumis melo] >KAA0056948.1 endonuclease 1 [Cucumis melo var. makuwa] >TYK26375.1 endonuclease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 394 bits (1012), Expect = 2.09e-136
Identity = 180/195 (92.31%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60
           MCVW DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 66  MCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 125

Query: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120
           TYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNLHHVWD
Sbjct: 126 TYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNLHHVWD 185

Query: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180
           RDIIL AL DYYDKDGGLLL+EL RNLTQGIWSNDV  WERC  VNSCVN+WA+EST LA
Sbjct: 186 RDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEESTDLA 245

Query: 181 CKWAYEGVEAGITLS 195
           CKWAYEGVEAGITLS
Sbjct: 246 CKWAYEGVEAGITLS 260

[Close]

BLAST of Cucsat.G14872 vs. NCBI nr
Match: XP_038895329.1 (LOW QUALITY PROTEIN: endonuclease 1-like [Benincasa hispida])

HSP 1 Score: 347 bits (890), Expect = 9.42e-118
Identity = 161/200 (80.50%), Postives = 171/200 (85.50%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60
           +CVW DQIRLQSKYRWASPLHYANTPD+CSF+YKRDCHN AGQPDMCVAGAIRNFTTQLT
Sbjct: 67  LCVWADQIRLQSKYRWASPLHYANTPDTCSFLYKRDCHNTAGQPDMCVAGAIRNFTTQLT 126

Query: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWF-----RRKSNL 120
           TYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGFESD GGNTIE   F     R++   
Sbjct: 127 TYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFESDEGGNTIESESFEIFNKRKRKXT 186

Query: 121 HHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADE 180
             VWDRDIIL A+ DYYDKD GLLL+EL RNLT GIWSNDV  WE C  VNSCVN+WA+E
Sbjct: 187 TQVWDRDIILTAVADYYDKDTGLLLEELQRNLTNGIWSNDVPAWESCVKVNSCVNKWAEE 246

Query: 181 STGLACKWAYEGVEAGITLS 195
           S  LACKWAYEGVEAG+TLS
Sbjct: 247 SIDLACKWAYEGVEAGMTLS 266

[Close]

BLAST of Cucsat.G14872 vs. NCBI nr
Match: XP_023000814.1 (endonuclease 1 [Cucurbita maxima])

HSP 1 Score: 329 bits (843), Expect = 9.71e-111
Identity = 150/196 (76.53%), Postives = 166/196 (84.69%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFTTQL 60
           MCVW DQIR  SKYRW SPLHY NTPD+ CSF+YKRDCHN A Q +MCVAGAIRNFTTQL
Sbjct: 62  MCVWADQIRRWSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTTQL 121

Query: 61  TTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVW 120
           T +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHVW
Sbjct: 122 TAFPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHVW 181

Query: 121 DRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGL 180
           DR+IIL AL DYYDKD GLLL++L RNLT GIWS++V  WERC  VNSC+N WA+ES  L
Sbjct: 182 DREIILTALADYYDKDTGLLLEDLQRNLTHGIWSDNVPTWERCVNVNSCINNWAEESIKL 241

Query: 181 ACKWAYEGVEAGITLS 195
           AC WAYEGVEAG+TLS
Sbjct: 242 ACTWAYEGVEAGMTLS 257

[Close]

BLAST of Cucsat.G14872 vs. NCBI nr
Match: KAG6584021.1 (Endonuclease 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019639.1 Endonuclease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 329 bits (843), Expect = 9.71e-111
Identity = 150/196 (76.53%), Postives = 165/196 (84.18%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFTTQL 60
           MCVW DQIR  SKYRW SPLHY NTPD+ CSF+YKRDCHN A Q +MCVAGAIRNFTTQL
Sbjct: 62  MCVWADQIRRSSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTTQL 121

Query: 61  TTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVW 120
           T +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHVW
Sbjct: 122 TAFPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHVW 181

Query: 121 DRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGL 180
           DR+IIL AL DYYDKD  LLL++L RNLT GIWS+DV  WERC  VNSC+N WA+ES  L
Sbjct: 182 DREIILTALADYYDKDTDLLLEDLQRNLTHGIWSDDVPTWERCVNVNSCINNWAEESIKL 241

Query: 181 ACKWAYEGVEAGITLS 195
           AC WAYEGVEAG+TLS
Sbjct: 242 ACTWAYEGVEAGMTLS 257

[Close]

BLAST of Cucsat.G14872 vs. ExPASy TrEMBL
Match: A0A0A0LUG7 (Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_1G072450 PE=3 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 7.87e-151
Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60
           MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60

Query: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120
           TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD
Sbjct: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120

Query: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180
           RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA
Sbjct: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180

Query: 181 CKWAYEGVEAGITLS 195
           CKWAYEGVEAGITLS
Sbjct: 181 CKWAYEGVEAGITLS 195

[Close]

BLAST of Cucsat.G14872 vs. ExPASy TrEMBL
Match: C3VEY2 (Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 PE=2 SV=2)

HSP 1 Score: 427 bits (1098), Expect = 7.54e-150
Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60
           MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 64  MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 123

Query: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120
           TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD
Sbjct: 124 TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 183

Query: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180
           RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA
Sbjct: 184 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 243

Query: 181 CKWAYEGVEAGITLS 195
           CKWAYEGVEAGITLS
Sbjct: 244 CKWAYEGVEAGITLS 258

[Close]

BLAST of Cucsat.G14872 vs. ExPASy TrEMBL
Match: A0A5D3DSP7 (Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00370 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 1.01e-136
Identity = 180/195 (92.31%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60
           MCVW DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 66  MCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 125

Query: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120
           TYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNLHHVWD
Sbjct: 126 TYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNLHHVWD 185

Query: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180
           RDIIL AL DYYDKDGGLLL+EL RNLTQGIWSNDV  WERC  VNSCVN+WA+EST LA
Sbjct: 186 RDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEESTDLA 245

Query: 181 CKWAYEGVEAGITLS 195
           CKWAYEGVEAGITLS
Sbjct: 246 CKWAYEGVEAGITLS 260

[Close]

BLAST of Cucsat.G14872 vs. ExPASy TrEMBL
Match: A0A1S3B4B0 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103486022 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 1.01e-136
Identity = 180/195 (92.31%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60
           MCVW DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 66  MCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 125

Query: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120
           TYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNLHHVWD
Sbjct: 126 TYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNLHHVWD 185

Query: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180
           RDIIL AL DYYDKDGGLLL+EL RNLTQGIWSNDV  WERC  VNSCVN+WA+EST LA
Sbjct: 186 RDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEESTDLA 245

Query: 181 CKWAYEGVEAGITLS 195
           CKWAYEGVEAGITLS
Sbjct: 246 CKWAYEGVEAGITLS 260

[Close]

BLAST of Cucsat.G14872 vs. ExPASy TrEMBL
Match: A0A6J1KL07 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111495151 PE=3 SV=1)

HSP 1 Score: 329 bits (843), Expect = 4.70e-111
Identity = 150/196 (76.53%), Postives = 166/196 (84.69%), Query Frame = 0


Query: 1   MCVWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFTTQL 60
           MCVW DQIR  SKYRW SPLHY NTPD+ CSF+YKRDCHN A Q +MCVAGAIRNFTTQL
Sbjct: 62  MCVWADQIRRWSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTTQL 121

Query: 61  TTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVW 120
           T +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHVW
Sbjct: 122 TAFPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHVW 181

Query: 121 DRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGL 180
           DR+IIL AL DYYDKD GLLL++L RNLT GIWS++V  WERC  VNSC+N WA+ES  L
Sbjct: 182 DREIILTALADYYDKDTGLLLEDLQRNLTHGIWSDNVPTWERCVNVNSCINNWAEESIKL 241

Query: 181 ACKWAYEGVEAGITLS 195
           AC WAYEGVEAG+TLS
Sbjct: 242 ACTWAYEGVEAGMTLS 257

The following BLAST results are available for this feature:

BLAST of Cucsat.G14872 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXA6	1.6e-69	59.18	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]
Q9C9G4	8.3e-58	51.28	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
F4JJL0	2.0e-56	54.31	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
F4JJL3	1.3e-50	50.00	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q8LDW6	3.5e-48	48.73	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]

BLAST of Cucsat.G14872 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001292654.1	1.56e-149	100.00	endonuclease 1 precursor [Cucumis sativus] >ACO72982.2 bifunctional nuclease pre...	[more]
XP_008442043.1	2.09e-136	92.31	PREDICTED: endonuclease 1 [Cucumis melo] >KAA0056948.1 endonuclease 1 [Cucumis m...	[more]
XP_038895329.1	9.42e-118	80.50	LOW QUALITY PROTEIN: endonuclease 1-like [Benincasa hispida]	[more]
XP_023000814.1	9.71e-111	76.53	endonuclease 1 [Cucurbita maxima]	[more]
KAG6584021.1	9.71e-111	76.53	Endonuclease 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019639.1 En...	[more]

BLAST of Cucsat.G14872 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUG7	7.87e-151	100.00	Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_1G072450 PE=3 SV=1	[more]
C3VEY2	7.54e-150	100.00	Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 PE=2 SV=2	[more]
A0A5D3DSP7	1.01e-136	92.31	Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3B4B0	1.01e-136	92.31	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103486022 PE=3 SV=1	[more]
A0A6J1KL07	4.70e-111	76.53	Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111495151 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 1..152 e-value: 8.0E-41 score: 140.5
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 1..154
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 1..140 e-value: 2.94474E-39 score: 131.738
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 1..165 e-value: 3.5E-50 score: 173.2
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 1..152
None	No IPR available	PANTHER	PTHR33146:SF14	ENDONUCLEASE 1	coord: 1..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G14872.T8	Cucsat.G14872.T8	mRNA
Cucsat.G14872.T4	Cucsat.G14872.T4	mRNA
Cucsat.G14872.T6	Cucsat.G14872.T6	mRNA
Cucsat.G14872.T1	Cucsat.G14872.T1	mRNA
Cucsat.G14872.T2	Cucsat.G14872.T2	mRNA
Cucsat.G14872.T3	Cucsat.G14872.T3	mRNA
Cucsat.G14872.T14	Cucsat.G14872.T14	mRNA
Cucsat.G14872.T13	Cucsat.G14872.T13	mRNA
Cucsat.G14872.T5	Cucsat.G14872.T5	mRNA
Cucsat.G14872.T10	Cucsat.G14872.T10	mRNA
Cucsat.G14872.T7	Cucsat.G14872.T7	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
biological_process	GO:0080187	floral organ senescence
biological_process	GO:0010150	leaf senescence
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0008309	double-stranded DNA exodeoxyribonuclease activity
molecular_function	GO:0004521	endoribonuclease activity
molecular_function	GO:0016891	endoribonuclease activity, producing 5'-phosphomonoesters
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity
molecular_function	GO:0043765	T/G mismatch-specific endonuclease activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds