Cucsat.G14680 (gene) Cucumber (B10) v3

BLAST of Cucsat.G14680 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 7.4e-166
Identity = 306/494 (61.94%), Postives = 373/494 (75.51%), Query Frame = 0

Query: 8   LLFLFLFFLSLFPFTLSRSSSHLS-PHSSASLDVSASLQQANQVLKFDPT-ASISFQQQV 67
           L  L +  LSLF  T   SS  LS P  +  LDV +SLQQ   +L  DPT +S++  +  
Sbjct: 7   LSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 66

Query: 68  HL---VPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSE 127
            L   V  NSS   SL+LH RD+   + HKDYKSL LSRL RDSSRV  I  ++ FA+  
Sbjct: 67  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 126

Query: 128 LKRSDLEPL---KTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN 187
           + RSDL+P+    T    EDL+TP++SG SQGSGEYFSR+GVG PAK  Y+VLDTGSD+N
Sbjct: 127 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVN 186

Query: 188 WLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSF 247
           W+QC+PC DCYQQ+DP+F+P SSS++ SL C + QC  LETS CR++KCLYQVSYGDGSF
Sbjct: 187 WIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSF 246

Query: 248 TVGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFSY 307
           TVGE  T+T+TFGNSG IN+VA+GCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+SFSY
Sbjct: 247 TVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSY 306

Query: 308 CLVDRDSSSSSDLEFNSA-APSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLF 367
           CLVDRDS  SS L+FNS         APLL++ K+DTFYYVGL+G SVGG+ + +P  +F
Sbjct: 307 CLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIF 366

Query: 368 QMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKK-TNGFALFDTCYDLSSQS 427
            +D SG GG+I+D GTA+TRLQTQAYN+LRDAF+  T  LKK ++  +LFDTCYD SS S
Sbjct: 367 DVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLS 426

Query: 428 RVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHY 487
            V +PTV+F F GGKSL LP KNYLIPVD  GTFCFAFAPT+SSLSIIGNVQQQGTR+ Y
Sbjct: 427 TVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITY 486

Query: 488 DLANSVVGFSPHKC 492
           DL+ +V+G S +KC
Sbjct: 487 DLSKNVIGLSGNKC 500

BLAST of Cucsat.G14680 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 3.0e-111
Identity = 251/495 (50.71%), Postives = 310/495 (62.63%), Query Frame = 0

Query: 8   LLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDP---TASISFQQQ 67
           L  L  FFLSL  F+   S   L P+S        SL  A+ V  F P   + S+   + 
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSH-------SLPCASPV-SFQPDSDSESLLESEF 68

Query: 68  VHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSELK 127
                S SS S +L L   D+L  + +K    L  SRL RDS RVKSI      A     
Sbjct: 69  ESGSDSESSSSITLNLDHIDAL--SSNKTPDELFSSRLQRDSRRVKSI---ATLAAQIPG 128

Query: 128 RSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ 187
           R+     +    P   S+ ++SG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC 
Sbjct: 129 RNVTHAPR----PGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA 188

Query: 188 PCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGC--RASKCLYQVSYGDGSFTVG 247
           PC  CY Q+DPIFDPR S ++A++PC S  C+ L+++GC  R   CLYQVSYGDGSFTVG
Sbjct: 189 PCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVG 248

Query: 248 EFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSL---TSQMKASSFSY 307
           +F TETLTF     +  VA+GCGHDNEGLFVG+AGLLGLG G LS    T       FSY
Sbjct: 249 DFSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSY 308

Query: 308 CLVDRDSSSS-SDLEFNSAAPSDSVN-APLLKSGKVDTFYYVGLTGMSVGG-QLLSIPPN 367
           CLVDR +SS  S + F +AA S      PLL + K+DTFYYVGL G+SVGG ++  +  +
Sbjct: 309 CLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTAS 368

Query: 368 LFQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQ 427
           LF++D  G GG+I+DSGT++TRL   AY  +RDAF      LK+   F+LFDTC+DLS+ 
Sbjct: 369 LFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNM 428

Query: 428 SRVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVH 487
           + V +PTV   F G   + LP  NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV 
Sbjct: 429 NEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVV 484

Query: 488 YDLANSVVGFSPHKC 492
           YDLA+S VGF+P  C
Sbjct: 489 YDLASSRVGFAPGGC 484

BLAST of Cucsat.G14680 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.1e-105
Identity = 209/490 (42.65%), Postives = 296/490 (60.41%), Query Frame = 0

Query: 8   LLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLK--FDPTASISFQQQV 67
           LL LF FFL            HL  H S+S  +S    Q   VL+     TA++      
Sbjct: 2   LLPLFFFFL------------HLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNT 61

Query: 68  HLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSELKR 127
           H    +SS  ++L+L  RD   +  ++++   + +R+ RD+ RV +I  R+   +     
Sbjct: 62  HFSDESSS-KYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSD 121

Query: 128 SDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQP 187
           S  E         D  + I+SG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQP
Sbjct: 122 SRYE-------VNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQP 181

Query: 188 CTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGEFV 247
           C  CY+Q+DP+FDP  S S+  + C S  C  +E SGC +  C Y+V YGDGS+T G   
Sbjct: 182 CKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLA 241

Query: 248 TETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMK---ASSFSYCLV 307
            ETLTF  + ++ +VA+GCGH N G+F+G+AGLLG+GGG +S   Q+      +F YCLV
Sbjct: 242 LETLTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLV 301

Query: 308 DRDSSSSSDLEF-NSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQMD 367
            R + S+  L F   A P  +   PL+++ +  +FYYVGL G+ VGG  + +P  +F + 
Sbjct: 302 SRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLT 361

Query: 368 DSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTI 427
           ++G GG+++D+GTA+TRL T AY   RD F S+T  L + +G ++FDTCYDLS    V +
Sbjct: 362 ETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRV 421

Query: 428 PTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLAN 487
           PTVSF F  G  L LP +N+L+PVD  GT+CFAFA + + LSIIGN+QQ+G +V +D AN
Sbjct: 422 PTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGAN 470

Query: 488 SVVGFSPHKC 492
             VGF P+ C
Sbjct: 482 GFVGFGPNVC 470

BLAST of Cucsat.G14680 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 6.4e-77
Identity = 157/347 (45.24%), Postives = 211/347 (60.81%), Query Frame = 0

Query: 151 GSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRSSSSFASLPC 210
           G GEY   + +G PA+PF  ++DTGSD+ W QCQPCT C+ Q+ PIF+P+ SSSF++LPC
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 150

Query: 211 ESQQCQALETSGCRASKCLYQVSYGDGSFTVGEFVTETLTFGNSGMINDVAVGCGHDNEG 270
            SQ CQAL +  C  + C Y   YGDGS T G   TETLTFG S  I ++  GCG +N+G
Sbjct: 151 SSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQG 210

Query: 271 LFVGS-AGLLGLGGGPLSLTSQMKASSFSYCLVDRDSSSSSDLEFNSAAPS---DSVNAP 330
              G+ AGL+G+G GPLSL SQ+  + FSYC+    SS+ S+L   S A S    S N  
Sbjct: 211 FGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTT 270

Query: 331 LLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQMD-DSGYGGIIVDSGTAITRLQTQAYN 390
           L++S ++ TFYY+ L G+SVG   L I P+ F ++ ++G GGII+DSGT +T     AY 
Sbjct: 271 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 330

Query: 391 TLRDAFVSRTPYLKKTNGFALFDTCYDL-SSQSRVTIPTVSFEFAGGKSLQLPPKNYLIP 450
           ++R  F+S+          + FD C+   S  S + IPT    F GG  L+LP +NY I 
Sbjct: 331 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS 390

Query: 451 VDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSVVGFSPHKC 492
             S G  C A   ++  +SI GN+QQQ   V YD  NSVV F+  +C
Sbjct: 391 -PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Cucsat.G14680 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.1e-75
Identity = 157/358 (43.85%), Postives = 207/358 (57.82%), Query Frame = 0

Query: 140 LSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDP 199
           + TP+ +    G GEY   V +G P   F  ++DTGSD+ W QC+PCT C+ Q  PIF+P
Sbjct: 85  IETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNP 144

Query: 200 RSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGEFVTETLTFGNSGMIND 259
           + SSSF++LPCESQ CQ L +  C  ++C Y   YGDGS T G   TET TF  S + N 
Sbjct: 145 QDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPN- 204

Query: 260 VAVGCGHDNEGLFVGS-AGLLGLGGGPLSLTSQMKASSFSYCLVDRDSSSSSDLEFNSAA 319
           +A GCG DN+G   G+ AGL+G+G GPLSL SQ+    FSYC+    SSS S L   SAA
Sbjct: 205 IAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAA 264

Query: 320 ---PSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDSGYGGIIVDSGTA 379
              P  S +  L+ S    T+YY+ L G++VGG  L IP + FQ+ D G GG+I+DSGT 
Sbjct: 265 SGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTT 324

Query: 380 ITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQ-SRVTIPTVSFEFAGGKSL 439
           +T L   AYN +  AF  +          +   TC+   S  S V +P +S +F GG  L
Sbjct: 325 LTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VL 384

Query: 440 QLPPKNYLIPVDSVGTFCFAFAPTTS-SLSIIGNVQQQGTRVHYDLANSVVGFSPHKC 492
            L  +N LI   + G  C A   ++   +SI GN+QQQ T+V YDL N  V F P +C
Sbjct: 385 NLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Cucsat.G14680 vs. NCBI nr
Match: XP_004137465.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus] >KGN64110.1 hypothetical protein Csa_013705 [Cucumis sativus])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 488/491 (99.39%), Postives = 489/491 (99.59%), Query Frame = 0

Query: 1   MALATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS 60
           MALATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS
Sbjct: 1   MALATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS 60

Query: 61  FQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL 120
           FQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL
Sbjct: 61  FQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL 120

Query: 121 SELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW 180
           SELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW
Sbjct: 121 SELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW 180

Query: 181 LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT 240
           LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT
Sbjct: 181 LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT 240

Query: 241 VGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFSYC 300
           VGEFV ETLTFGNSGMIN+VAVGCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSFSYC
Sbjct: 241 VGEFVIETLTFGNSGMINNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYC 300

Query: 301 LVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQM 360
           LVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQM
Sbjct: 301 LVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQM 360

Query: 361 DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT 420
           DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT
Sbjct: 361 DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT 420

Query: 421 IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA 480
           IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA
Sbjct: 421 IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA 480

Query: 481 NSVVGFSPHKC 491
           NSVVGFSPHKC
Sbjct: 481 NSVVGFSPHKC 491

BLAST of Cucsat.G14680 vs. NCBI nr
Match: XP_008457107.2 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like, partial [Cucumis melo])

HSP 1 Score: 942 bits (2435), Expect = 0.0
Identity = 478/491 (97.35%), Postives = 484/491 (98.57%), Query Frame = 0

Query: 1   MALATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS 60
           + +ATSNLL LFLFF SLFPF LSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS
Sbjct: 46  LTMATSNLLTLFLFFFSLFPFALSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS 105

Query: 61  FQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL 120
           FQQQV LVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL
Sbjct: 106 FQQQVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL 165

Query: 121 SELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW 180
           SELKRSDLEPLKTEILPEDLSTPI+SGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW
Sbjct: 166 SELKRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW 225

Query: 181 LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT 240
           LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT
Sbjct: 226 LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT 285

Query: 241 VGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFSYC 300
           VGEFVTETLTFGNSGMI +VAVGCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSFSYC
Sbjct: 286 VGEFVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYC 345

Query: 301 LVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQM 360
           LVDRDSSSSSDLEFNSAAP+DSVNAPLLKSG+VDTFYYVGLTGMSVGGQLLSIPPNLFQM
Sbjct: 346 LVDRDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPNLFQM 405

Query: 361 DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT 420
           DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT
Sbjct: 406 DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT 465

Query: 421 IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA 480
           IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA
Sbjct: 466 IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA 525

Query: 481 NSVVGFSPHKC 491
           NSVVGFSPHKC
Sbjct: 526 NSVVGFSPHKC 536

BLAST of Cucsat.G14680 vs. NCBI nr
Match: TYJ98983.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 478/488 (97.95%), Postives = 482/488 (98.77%), Query Frame = 0

Query: 4   ATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQ 63
           ATSNLL LFLFF SLFPF LSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQ
Sbjct: 2   ATSNLLTLFLFFFSLFPFALSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQ 61

Query: 64  QVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSEL 123
           QV LVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSEL
Sbjct: 62  QVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSEL 121

Query: 124 KRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC 183
           KRSDLEPLKTEILPEDLSTPI+SGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC
Sbjct: 122 KRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC 181

Query: 184 QPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGE 243
           QPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGE
Sbjct: 182 QPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGE 241

Query: 244 FVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFSYCLVD 303
           FVTETLTFGNSGMI +VAVGCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSFSYCLVD
Sbjct: 242 FVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVD 301

Query: 304 RDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDS 363
           RDSSSSSDLEFNSAAP+DSVNAPLLKSG+VDTFYYVGLTGMSVGGQLLSIPPNLFQMDDS
Sbjct: 302 RDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDS 361

Query: 364 GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPT 423
           GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPT
Sbjct: 362 GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPT 421

Query: 424 VSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSV 483
           VSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSV
Sbjct: 422 VSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSV 481

Query: 484 VGFSPHKC 491
           VGFSPHKC
Sbjct: 482 VGFSPHKC 489

BLAST of Cucsat.G14680 vs. NCBI nr
Match: XP_038894046.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Benincasa hispida])

HSP 1 Score: 867 bits (2240), Expect = 1.55e-315
Identity = 444/493 (90.06%), Postives = 464/493 (94.12%), Query Frame = 0

Query: 4   ATSNLLFLF---LFFLSLFPFTLSRSSSHLS--PHSSASLDVSASLQQANQVLKFDPTAS 63
           AT NL  LF   LFF SLFPFTL R+S  LS    SSASLDVSASLQQAN VLKFDP   
Sbjct: 2   ATPNLPPLFAVALFFFSLFPFTLGRTSPELSRSSSSSASLDVSASLQQANHVLKFDPKPF 61

Query: 64  ISFQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEF 123
           +SFQQQV +VPSNSSFSFSLQLHPRD+LHNAGHKDYK+LVLSRL RDSSRVKS+YDRL+F
Sbjct: 62  MSFQQQVQVVPSNSSFSFSLQLHPRDALHNAGHKDYKTLVLSRLVRDSSRVKSLYDRLQF 121

Query: 124 ALSELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDI 183
           ALSELK SDL PLKTEILPEDLSTPI+SGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDI
Sbjct: 122 ALSELKSSDLHPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDI 181

Query: 184 NWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGS 243
           NWLQCQPCTDCYQQTD IFDPR+SS+FASLPCESQQCQALETSGCRASKCLYQVSYGDGS
Sbjct: 182 NWLQCQPCTDCYQQTDSIFDPRTSSTFASLPCESQQCQALETSGCRASKCLYQVSYGDGS 241

Query: 244 FTVGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFS 303
           FTVGEFVTETL+FGNSG IN+VA+GCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSFS
Sbjct: 242 FTVGEFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFS 301

Query: 304 YCLVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLF 363
           YCLVDRDSSSSS LEFNSAA SDSVNAPLLKSG+VDTFYY+GL G+SVGGQ+LSIPPNLF
Sbjct: 302 YCLVDRDSSSSSFLEFNSAASSDSVNAPLLKSGRVDTFYYIGLIGLSVGGQILSIPPNLF 361

Query: 364 QMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSR 423
           QMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSR
Sbjct: 362 QMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSR 421

Query: 424 VTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYD 483
           VTIPTVSF+FAGGKSLQLPPKNYLIPVDS GTFCFAFAPTTSSLSIIGNVQQQGTRVH+D
Sbjct: 422 VTIPTVSFQFAGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVHFD 481

Query: 484 LANSVVGFSPHKC 491
           LANSVVGFSP+KC
Sbjct: 482 LANSVVGFSPNKC 494

BLAST of Cucsat.G14680 vs. NCBI nr
Match: XP_023519500.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846 bits (2185), Expect = 4.16e-307
Identity = 430/494 (87.04%), Postives = 463/494 (93.72%), Query Frame = 0

Query: 4   ATSNLLFLF---LFFLSLFPFTLSRSS---SHLSPHSSASLDVSASLQQANQVLKFDPTA 63
           AT+NLL LF   +FF SL PFTL R+S   SH S  SSA LDVSASL+QANQVLKFDP A
Sbjct: 2   ATANLLPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKA 61

Query: 64  SISFQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLE 123
            +SFQQQ  +VP NSS SFSLQLH RD+L NAGHKDYKSLVLSRL RDSSRVKS+ DRLE
Sbjct: 62  LLSFQQQDEVVPGNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLE 121

Query: 124 FALSELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSD 183
           F+LS+LKRSDL+PL+TEILPEDLSTPI+SGTSQGSGEYFSR+GVG+PAKPFYMVLDTGSD
Sbjct: 122 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD 181

Query: 184 INWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDG 243
           INWLQCQPCTDCYQQTDPIFDPR+SSSFASLPCES QCQALETSGCRA+KCLYQV+YGDG
Sbjct: 182 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG 241

Query: 244 SFTVGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSF 303
           SFTVGEFVTE+L+FGNSG IN+VA+GCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSF
Sbjct: 242 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 301

Query: 304 SYCLVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNL 363
           SYCLVDRDSSSSS LEFNSAAPSDSVNAPLLKSG+VDTFYYVGL G+SVGGQLLSIPP+L
Sbjct: 302 SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLKSGRVDTFYYVGLVGLSVGGQLLSIPPSL 361

Query: 364 FQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQS 423
           FQMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNGFALFDTCYDLSSQS
Sbjct: 362 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS 421

Query: 424 RVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHY 483
           RVTIPT+SF+FAGG SLQLPPKNYLIPVDSVGTFC AFAPTTSSLSIIGNVQQQGTRVH+
Sbjct: 422 RVTIPTLSFQFAGGNSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 481

Query: 484 DLANSVVGFSPHKC 491
           DLANS+VGFSP+KC
Sbjct: 482 DLANSLVGFSPNKC 495

BLAST of Cucsat.G14680 vs. ExPASy TrEMBL
Match: A0A0A0LQD2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042220 PE=3 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 488/491 (99.39%), Postives = 489/491 (99.59%), Query Frame = 0

Query: 1   MALATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS 60
           MALATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS
Sbjct: 1   MALATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS 60

Query: 61  FQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL 120
           FQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL
Sbjct: 61  FQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL 120

Query: 121 SELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW 180
           SELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW
Sbjct: 121 SELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW 180

Query: 181 LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT 240
           LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT
Sbjct: 181 LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT 240

Query: 241 VGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFSYC 300
           VGEFV ETLTFGNSGMIN+VAVGCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSFSYC
Sbjct: 241 VGEFVIETLTFGNSGMINNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYC 300

Query: 301 LVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQM 360
           LVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQM
Sbjct: 301 LVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQM 360

Query: 361 DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT 420
           DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT
Sbjct: 361 DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT 420

Query: 421 IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA 480
           IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA
Sbjct: 421 IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA 480

Query: 481 NSVVGFSPHKC 491
           NSVVGFSPHKC
Sbjct: 481 NSVVGFSPHKC 491

BLAST of Cucsat.G14680 vs. ExPASy TrEMBL
Match: A0A1S3C5F6 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103496838 PE=3 SV=1)

HSP 1 Score: 942 bits (2435), Expect = 0.0
Identity = 478/491 (97.35%), Postives = 484/491 (98.57%), Query Frame = 0

Query: 1   MALATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS 60
           + +ATSNLL LFLFF SLFPF LSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS
Sbjct: 46  LTMATSNLLTLFLFFFSLFPFALSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASIS 105

Query: 61  FQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL 120
           FQQQV LVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL
Sbjct: 106 FQQQVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFAL 165

Query: 121 SELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW 180
           SELKRSDLEPLKTEILPEDLSTPI+SGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW
Sbjct: 166 SELKRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINW 225

Query: 181 LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT 240
           LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT
Sbjct: 226 LQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFT 285

Query: 241 VGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFSYC 300
           VGEFVTETLTFGNSGMI +VAVGCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSFSYC
Sbjct: 286 VGEFVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYC 345

Query: 301 LVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQM 360
           LVDRDSSSSSDLEFNSAAP+DSVNAPLLKSG+VDTFYYVGLTGMSVGGQLLSIPPNLFQM
Sbjct: 346 LVDRDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPNLFQM 405

Query: 361 DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT 420
           DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT
Sbjct: 406 DDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVT 465

Query: 421 IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA 480
           IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA
Sbjct: 466 IPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLA 525

Query: 481 NSVVGFSPHKC 491
           NSVVGFSPHKC
Sbjct: 526 NSVVGFSPHKC 536

BLAST of Cucsat.G14680 vs. ExPASy TrEMBL
Match: A0A5D3BI72 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001860 PE=3 SV=1)

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 478/488 (97.95%), Postives = 482/488 (98.77%), Query Frame = 0

Query: 4   ATSNLLFLFLFFLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQ 63
           ATSNLL LFLFF SLFPF LSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQ
Sbjct: 2   ATSNLLTLFLFFFSLFPFALSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQ 61

Query: 64  QVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSEL 123
           QV LVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSEL
Sbjct: 62  QVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSEL 121

Query: 124 KRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC 183
           KRSDLEPLKTEILPEDLSTPI+SGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC
Sbjct: 122 KRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC 181

Query: 184 QPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGE 243
           QPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGE
Sbjct: 182 QPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGE 241

Query: 244 FVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFSYCLVD 303
           FVTETLTFGNSGMI +VAVGCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSFSYCLVD
Sbjct: 242 FVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVD 301

Query: 304 RDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDS 363
           RDSSSSSDLEFNSAAP+DSVNAPLLKSG+VDTFYYVGLTGMSVGGQLLSIPPNLFQMDDS
Sbjct: 302 RDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDS 361

Query: 364 GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPT 423
           GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPT
Sbjct: 362 GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPT 421

Query: 424 VSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSV 483
           VSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSV
Sbjct: 422 VSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSV 481

Query: 484 VGFSPHKC 491
           VGFSPHKC
Sbjct: 482 VGFSPHKC 489

BLAST of Cucsat.G14680 vs. ExPASy TrEMBL
Match: A0A6J1E815 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431581 PE=3 SV=1)

HSP 1 Score: 841 bits (2173), Expect = 1.30e-305
Identity = 428/493 (86.82%), Postives = 463/493 (93.91%), Query Frame = 0

Query: 4   ATSNLLFLFLF---FLSLFPFTLSRSSSHLSPHSSAS--LDVSASLQQANQVLKFDPTAS 63
           AT+NLL LF F   F SL PFTL R+S  LS  SS+S  LDVSASL+QANQVLKFDP A 
Sbjct: 2   ATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKAL 61

Query: 64  ISFQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEF 123
           +SFQQQ  +VP NSS SFSLQLH RD+L NAGH+DYKSLVLSRL RDSSRVKS+ DRLEF
Sbjct: 62  LSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEF 121

Query: 124 ALSELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDI 183
           +LS+LKRSDL+PL+TEILPEDLSTPI+SGTSQGSGEYFSR+GVG+PAKPFYMVLDTGSDI
Sbjct: 122 SLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDI 181

Query: 184 NWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGS 243
           NWLQCQPCTDCYQQTDPIFDPR+SSSFASLPCES QCQALETSGCRA+KCLYQV+YGDGS
Sbjct: 182 NWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGS 241

Query: 244 FTVGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSFS 303
           FTVGEFVTE+L+FGNSG IN+VA+GCGHDNEGLFVGSAGLLGLGGG LSLTSQMKASSFS
Sbjct: 242 FTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFS 301

Query: 304 YCLVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLF 363
           YCLVDRDSSSSS LEFNSAAPSDSVNAPLL+SG+VDTFYYVGL G+SVGGQLLSIPP+LF
Sbjct: 302 YCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLF 361

Query: 364 QMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSR 423
           QMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNGFALFDTCYDLSSQSR
Sbjct: 362 QMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSR 421

Query: 424 VTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYD 483
           VTIPT+SF+FAGGKSLQLPPKNYLIPVDSVGTFC AFAPTTSSLSIIGNVQQQGTRVH+D
Sbjct: 422 VTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFD 481

Query: 484 LANSVVGFSPHKC 491
           LANS+VGFSP+KC
Sbjct: 482 LANSLVGFSPNKC 494

BLAST of Cucsat.G14680 vs. ExPASy TrEMBL
Match: A0A6J1KJJ8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495193 PE=3 SV=1)

HSP 1 Score: 836 bits (2159), Expect = 1.84e-303
Identity = 425/494 (86.03%), Postives = 461/494 (93.32%), Query Frame = 0

Query: 4   ATSNLLFLF---LFFLSLFPFTLSRSS---SHLSPHSSASLDVSASLQQANQVLKFDPTA 63
           AT+NL  LF   +FF SL PFTL R+S   SH S  SSA LDVSASL+QANQVLKFDP A
Sbjct: 2   ATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKA 61

Query: 64  SISFQQQVHLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLE 123
            +SFQQQ  +VP+NSS SFSLQLH RD+L NAGHKDYKSLVLSRL RDSSRVKS+ DRLE
Sbjct: 62  LLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLE 121

Query: 124 FALSELKRSDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSD 183
           F+LS+LKRSDL+PL+TEILPEDLSTPI+SGTSQGSGEYFSR+GVGQPAKPFYMVLDTGSD
Sbjct: 122 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSD 181

Query: 184 INWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDG 243
           INWLQCQPCTDCYQQTDPIFDPR+SSSFASL CES QCQALETSGCRA+KCLYQV+YGDG
Sbjct: 182 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDG 241

Query: 244 SFTVGEFVTETLTFGNSGMINDVAVGCGHDNEGLFVGSAGLLGLGGGPLSLTSQMKASSF 303
           SFTVGEFVTE+L+FGNSG IN+VA+GCGHDNEGLFVGSAGLLGLGGG LSL SQMKASSF
Sbjct: 242 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASSF 301

Query: 304 SYCLVDRDSSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNL 363
           SYCLVDRDSSSSS LEFNSAAPSD+VNAPLL+SG+VDTFYYVGL G+SVGGQLLSIPP+L
Sbjct: 302 SYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 361

Query: 364 FQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQS 423
           FQMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNG ALFDTCYDLSSQS
Sbjct: 362 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQS 421

Query: 424 RVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHY 483
           RVTIPT+SF+FAGGKSLQLPPKNYLIPVDSVGTFC AFAPTTSSLSIIGNVQQQGTRVH+
Sbjct: 422 RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 481

Query: 484 DLANSVVGFSPHKC 491
           DLANS++GFSP+KC
Sbjct: 482 DLANSLLGFSPNKC 495