Cucsat.G14554 (gene) Cucumber (B10) v3

Overview
NameCucsat.G14554
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionprotein MIS12 homolog
Locationctg1869: 1093280 .. 1095708 (+)
RNA-Seq ExpressionCucsat.G14554
SyntenyCucsat.G14554
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACTCTTGATGTCAAGTTCCAGTAGAACATGAAACTCTTGATGTCAAGTTCCAGTAGAACATGAAACTCTTGATGTCAAGTTCTAGTAGAATATAAAATATTAGGTCGCAAACAACGTGAGTCATTTATATATATAATTAGACAAAAGTCTTTTATGAGGTAAGAACTAATATACCATTTTTGTTTAATATCATATCACCAAGCAAGGGAGATGCAAGCCAACATACACTGTTCGTTCATCTATAACATACTGAACAATTGTTGAAGCCCAAAAACAAAGCATGGAAAATAGATGGTTACATTCAGAAGGTAATATTTCAAATGTTTATCTAATCATAGATTCAGTAAACTGCACAATAATCTACAATCGTTTTTTTCTGATATGCAATACACATCCACTCATGGTCATGGTTGAAAACTATAGATGCTCAAGGAATGACATGACCAGTGCATGCAATCTGGTGAAGGCAATTGTTATGGATATAAAAAATGTTAATTTTAGGGTGAAGGCAAAAAACATGACCAGTGCATGCAATGTAGTGAAACTACGAGTTTAAGAAGTGTAGACAACCATCAAGCCAAAACTCAAAACAAGTGATTGTTTTTGTATATCCTGAAAACAAGTTGGTATTAGAAATCAAACCTGAAAAATACGTGGATCCCTTGCTTTCGTGAATGAAGGGAGGTTCCACCCAGTCATATGCTTTGACATGAAGAGAACCATAGAGAACTTTGCTGAACACAGTCATCCCAGGATGGTCGTGAAGTGGGATGACTGAAGAAGTTGGAAAGCAAAATATACATATCTGCTGATATTAAGTACATACCATAAGCATACTTATTACAATAGGGGCTTCAAAAATAACTAACAGCCCGTTGGTAGTTTAAGTCAACCATAGATACAGTTTCTGGTAATTTTGACACTAACTTCTCCAAATAAAAACTAGGTCACAGTGTGGAAAATTCAAAAGGTAATCAATTCTCAGATGCAAGAGGCCTATGCCTCAGGTTTTTCTCCCTGAACAAAACTCAACAATGAATATCGTAGAACCAAAATCCTATTGCCAAAAACATTTCCCTAGAAATTAAAATTGAAAAACCTGTGAATCCAACTTTCTGGTTGCTAAATAATGAATGAACAAGACCTGTTTGAGAAAAGGGATGCATGGGACTGACCGTAAAATTTTCACATTCAAATATGTCAACGTATGTTATTGGCTGAGCCCATCGAGCTATCCTATTCAATTGGCTCAGTCCAAAAAGTCCATGCCCTCGGTCGTCATCAGCATTCTCTTCTTTAAGCCCAACATCCCCCACGCCAACCGTATCTGTGAATAGAGCATATAAATGGGATGTGTTAAAGAGTGGAGTCTAACGCAGTACATAATGTAGTATGTCCTACTTGAAGAAAACTTTAGTTTCTGAAACAGTATTCTTTAGAACAGCAAAAGAACACAATAATCAATTGTCTTGTAATAAATCATTCCTCAAACTCGGAATCAGGGAGAATAAGAGAATACAAACATATACACACGCCCATTGATTGAGATCTCCAATCATCATTATTTCCAATATAGTAAGTAAAGGTAACATCAAAACGTTACCATACTCCACTCCAAACAATTGAAGGATGATTGAAGTTGATGCAGCGGACAAAGTATTAGGCTAAATCCATGGCTAAAAAAGAGAAGTCTATCCCTTAAGATGTCTAGAGAACACTCAAGAACAAAGGTTCTATATTCAGCTTCCAATTTAGTTTAAGAAATTCCCAAATGGCAATTCACGAACTAACCAAGGATCCAATCCATCAATTAAACCTCCAAAACTAAGCAAACCCATGAATGAATCACTTGAATTATGAACCCAGATGGGAAATTAACAAGAATCACAAGAAGAAACAAAAGGGTAGTTGGAAGACCAAATGTAGAAGATAATGCAGTATAAACTTCAAAGCAAGAAAGAGAAATTACCCAAAAGGGAACATAGATTCTTGATAGTTTGAGAAGAAAGTGGAGAGTTCGCGGGAGTTAAACTCTTTTTACAAAGGTCGTAGATAGCCTGAATTTTGGAGCTCTTCTTGTGGCTCATGCTCAAGAACCGAAGAAACACTGAGCAATCCACCTTGTTTTGCAAAAATAATCTCATCTTATCCGTCGCTTTTTGGGTTTTTGAGAACATTTTGAAATCAACAGAAAAAAGAAAAAAAAATTAAATGCAAATTTAGATTTTGGAATGAGATTATGAAATACCGACACGCATCGCAGCCGATGCAATGATGTAATTATCCTCAACAAAATAAAATACATAGAGAGAGAGTTCATATTAATTATAAATAGTTTTAACTTTTTAACTATATGCTTGAAATTATCATTTTCTTGTTGTTTAAATAAAATAAAAATTTATGCAATAACCAATACCTAAAAATACAAACACATATCTAACATTATTTACATCATCCTTAATACTAAATGATCTCTTAAATTAACTTAGTGATAATTGACATGACATTCGAATCTGAATGTATGAGATTTCATCTCAAACTTATCATTATTTTACTAAAAATTTATTGTAATTTTGTAGGAAAAAGAGAGCAACTAATGGTATAAAGTTTATTTTTCAAATATTTTGATAGCTTTTAGTTGAAAATGAAAATTCATCAAAGTATTTTTCAAGTCTATTGTTAAAAAGATCAATAACGAAGATATTATGGATTTGATACATATAACCGAGACACTCATCATCACTGGTATAAAAAGTAATAATAATGATAATAACTCACATACAATATTTCTAATACTGATTTTTTTTCTTCAAATTTTCATTATTATTTTTAAGATTTGATCTAACTACTAAAAAATTAGGTTGTAAAATTAAATTTGAAGCTTATTTAAATTAGGGACTAATTTGTAATTGAACTAAATTATTGGGTTAAGGTTAAGTCCGCTCCATGTCTCCATCAGTAGCCATTACCATTTTGGCGGCAAAATTCCTCCATTTATGCCCAAGCTTTGGAGCTTCCCATTTGAATTTCTTCTCGTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTGGGTAAGAAGGTTTTTCAGGACGCAAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCGTATTGTTCTCTCTGTTTTCTATTTCTTTAATTTAGGTTTTTCAATTTTGTTCGGTTAGGTTAATTTTGTTGATTCTTGTTAATTCTTTTTCCCTTCAGACAAGCGTCGGCTGCCTTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGTAAGGTTTAATTTGTATTATGTTATTTAGCATTTTCTTGTTAATTTCTGAATTCTTAAGTTTGTACTCTTAAGCACTTCTTTGAATTGTTCACATTTTCGCCTCGTTTTATTCAGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAGATGTAGGTTCTTCTTTCTTAATGAGGCTCCTTGGCCAAAGAATAAAAATATCATGTTGGTATCAATATCTGAATTTTAGTTTGTAGTTTATCGATTATATATCAACCTTGATAAATATTTATGTAAAGCAATAAATTAACCGAAATTAGCTATTGAATTGCTTTTGCTTTCAAACTAAGTTGTAGATCTTGCTTTTAAGTAATATTCATATTGAGATTAATAGATGACATCTTTTTATGTTTTATGTGTTTATAAAGGATAGTTGAGATGTTTATAAACCCTTAATATTGATGTCAAATATTCATATATATATATATAACTTTGCCGGTTTCAAAAGTTCTCTATAAATATAACTTACATGTAAATGACCGGTTGCTTATGCAGCGAGTTTCCCATTTATTTTGTACTGACTTGATAGCTTCCTCAATTTTTGGTTCTGGGACGAACAAATTTTGAGGAGAAAGCGCAGTTACTTACTGTGTATCATTGAGTTTCAATTTTTGTTCCAAATATATTCATTGCTACTTTCAGATATAATCACAAGTTTCCATATTTTCAGACCTCAGAGCTTCCTCATTTTGTTAGCAATAACTCAACGTGGTTCCATTGTAACTAGAATGTGGTCGTGACGGTAAAAGATTCTTTTTAGCTCTAAAAGGAATGCGACCAAATGCCTGCAGCACTTATAAAAATCTGTCACTGGGGCATCATGCCTTTTCTTTTTCAATTACATTTGAAATCTCCTATGTAAGATCCAGTTCGATTCCTTTCATTTTGCACCAATATAGTTTACTTTGGGTTGGGCAACTTCTGTCTGTTTCACTGGCATCTCATTGGAATCCCCAATATCTTATTGTCAGGATGCTAAATTATCAATGGTTCCCATCTTGTTTGAGAACTTTGAGTTATGGCTAATTGAGTCTATGTGTTCTTTTAATCGAGTCTGTGTTCTTTTACTACTTTTTCTTCTTTCTAATGCAATAGCTAATAAACATATTTCTGATTCAGATCTAAATATTTTCTTTTAAGTTGTCTTAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTATTGAATATGTCGTATGTTTAACACGTGCGTTTAAGTAATTCATATCTCATGAGGAAATATATTCTTCAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGGTAACTAAGTTGTGCTTAAAACCAATTTTTTCTTCCTGCACTCCTGGGTTTACATGCACACTACTCTACTAGTACACTCGTGAAGGCTTCCTCCGAATGAAATATACAAATAATGGCCTCCTCCGGCGGATCATCCTGGTTTGCATTTCTCATGGTGACACACTCAAATAATTCCAAATGACACTTAAGTTAACATCTGTAAAATCATTTCATTTAGGCAATTTTTATATTACACTAAACTCTAACAGTCTCATTTTCTGTTCATTCCATAAAAGAGGAGCTTCTTGCAGTATGTGAATAAGGCAACTTCTCTTGCTATTTTCATTTTGAATGCCACTTGAACAAAGTTTATTCTTTAGTTATTAAATTTCGTTGTACTCAAACTTCAACAGTCGGTAGGTAAAAGTTGTTCATAATAGTAGGAAGCAGAATTCTAACATGTATCAATTTCTTGCAGAAATGATCAGAACTGCATCGGAGCTTCGTGTGAAAATAGGAAAGCTGAAGAAAAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGGTTCATACAAATGGAGACATCTCTCACCATCACAAGGGTAGGATTTTCAAGCTACTTTATTATCAATGAAGCTGGTTTTAGCTTTTTTGAAGCTGAATATCATCTCACATGCTCGTTGATGCTTGTTTCTGTTGTGCAGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGAAGTTATTTCACCATGACTTCAATCTTACTGTATATCATGCTTGCTTACGAAACTTGTCACTATGAGTTCTTCAATGTTTAAACTTTATTTAGCAGTTGTGAGATATTCTAAACAAAATTTGTATTATATATCATTTTGGATTGATGACTATCCTTGGTAATCTTCTTTGAGAGTTTGCAGATGAGCCATGAGTAGTTGGCTCAATCCTTCCTATGGTTTGCATTACACTTTTCAAGCTTAT

Coding sequence (CDS)

ATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCACAAGCGTCGGCTGCCTTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAGATGTAGGTTCTTCTTTCTTAATGAGGCTCCTTGGCCAAAGAATAAAAATATCATGTTGGTATCAATATCTGAATTTTAGTTTGTAG

Protein sequence

MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDVGSSFLMRLLGQRIKISCWYQYLNFSL
Homology
[Close]
BLAST of Cucsat.G14554 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 4.6e-28
Identity = 75/185 (40.54%), Postives = 123/185 (66.49%), Query Frame = 0 

Query: 9   GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD--HDVDLD 68
           GI++VR L+   +  RL +WE Y L  CF+VP+GF LP  +ES   +S+  D  +D++LD
Sbjct: 63  GIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLELD 122

Query: 69  TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 128
            ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+EM
Sbjct: 123 AELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFKEM 182

Query: 129 IRTASELRVKIGKLK-KRTEETKLAIVEKVHTNGDISHHHKGFS----NAKLDDIQEFLS 187
            + ASELR  + +LK +R + ++ A V++      + +H K FS    + KL+D+++F +
Sbjct: 183 TKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKFQA 238

[Close]
BLAST of Cucsat.G14554 vs. NCBI nr
Match: XP_004138204.3 (protein MIS12 homolog isoform X1 [Cucumis sativus])

HSP 1 Score: 345 bits (885), Expect = 1.76e-118
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0 

Query: 9   GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 68
           GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 69  LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 128
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180

Query: 129 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 186
           TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238

[Close]
BLAST of Cucsat.G14554 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 321 bits (822), Expect = 7.18e-109
Identity = 168/179 (93.85%), Postives = 172/179 (96.09%), Query Frame = 0 

Query: 9   GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 68
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 69  LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 128
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 129 TASELRVKIGKLKKR-TEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 186
           TASELR KIGK+KKR TEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]
BLAST of Cucsat.G14554 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 320 bits (820), Expect = 1.45e-108
Identity = 168/179 (93.85%), Postives = 172/179 (96.09%), Query Frame = 0 

Query: 9   GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 68
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 69  LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 128
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 129 TASELRVKIGKLKKR-TEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 186
           TASELR KIGK+KKR TEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]
BLAST of Cucsat.G14554 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 291 bits (746), Expect = 7.31e-97
Identity = 152/179 (84.92%), Postives = 165/179 (92.18%), Query Frame = 0 

Query: 9   GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 68
           GIS+VRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+D+SPGVTSISHD+DV LDTE
Sbjct: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150

Query: 69  LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 128
           LD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ 
Sbjct: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMG 210

Query: 129 TASELRVKIGKLKKRT-EETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 186
           TASELR KIGKLKKR  EE+KL  VEKVHTNGDISH+H G SNAKLDDI+ FL DLK +
Sbjct: 211 TASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 269

[Close]
BLAST of Cucsat.G14554 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 288 bits (737), Expect = 6.66e-96
Identity = 152/187 (81.28%), Postives = 166/187 (88.77%), Query Frame = 0 

Query: 3   SPRFIQGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD-- 62
           S   I GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+DESP VTS+S D  
Sbjct: 55  SQDLILGISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCL 114

Query: 63  HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVN 122
           +DVDLDTELD LRNKLSEVRKEN+VLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN
Sbjct: 115 YDVDLDTELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVN 174

Query: 123 DMFQEMIRTASELRVKIGKLKKRT-EETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEF 182
           +MFQEM+ TASELR KIGKLKKR  EE+KL  VEK+HTNGDISHHHKGFSNAKLDDIQEF
Sbjct: 175 EMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEF 234

Query: 183 LSDLKKI 186
           L+DLK +
Sbjct: 235 LADLKAL 241

[Close]
BLAST of Cucsat.G14554 vs. ExPASy TrEMBL
Match: A0A0A0LPL2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1)

HSP 1 Score: 342 bits (878), Expect = 9.94e-118
Identity = 177/178 (99.44%), Postives = 177/178 (99.44%), Query Frame = 0 

Query: 9   GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 68
           GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 69  LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 128
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180

Query: 129 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 186
           TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLS LKKI
Sbjct: 181 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSGLKKI 238

[Close]
BLAST of Cucsat.G14554 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 321 bits (822), Expect = 3.48e-109
Identity = 168/179 (93.85%), Postives = 172/179 (96.09%), Query Frame = 0 

Query: 9   GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 68
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 69  LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 128
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 129 TASELRVKIGKLKKR-TEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 186
           TASELR KIGK+KKR TEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]
BLAST of Cucsat.G14554 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 320 bits (820), Expect = 7.01e-109
Identity = 168/179 (93.85%), Postives = 172/179 (96.09%), Query Frame = 0 

Query: 9   GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 68
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 69  LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 128
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 129 TASELRVKIGKLKKR-TEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 186
           TASELR KIGK+KKR TEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]
BLAST of Cucsat.G14554 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 283 bits (724), Expect = 3.06e-94
Identity = 150/187 (80.21%), Postives = 164/187 (87.70%), Query Frame = 0 

Query: 3   SPRFIQGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD-- 62
           S   I GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+DESP VTS+S D  
Sbjct: 55  SQDLILGISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCL 114

Query: 63  HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVN 122
           +DVDLDTELD LRNKLSEVRKENIVLNQELQALERQ ASSNSQ++ FNEALQLYE++SVN
Sbjct: 115 YDVDLDTELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVN 174

Query: 123 DMFQEMIRTASELRVKIGKLKKRT-EETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEF 182
           +MFQEM+ TASELR KIGKLKKR  EE+KL  VEK+HTNGD SHHHKGFSNAKLDDIQEF
Sbjct: 175 EMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEF 234

Query: 183 LSDLKKI 186
           L+DLK +
Sbjct: 235 LADLKAL 241

[Close]
BLAST of Cucsat.G14554 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 281 bits (719), Expect = 1.76e-93
Identity = 150/187 (80.21%), Postives = 163/187 (87.17%), Query Frame = 0 

Query: 3   SPRFIQGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD-- 62
           S   I GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+DESP VTS+S D  
Sbjct: 55  SQDLILGISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCL 114

Query: 63  HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVN 122
           +DVDLDTELD LR  LSEVRKENIVLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN
Sbjct: 115 YDVDLDTELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVN 174

Query: 123 DMFQEMIRTASELRVKIGKLKKRT-EETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEF 182
           +MFQEM+ TASELR KIGKLKKR  EE+KL  VEK+HTN DISHHHKGFSNAKLDDIQEF
Sbjct: 175 EMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEF 234

Query: 183 LSDLKKI 186
           L+DLK +
Sbjct: 235 LADLKTL 241

The following BLAST results are available for this feature:
BLAST of Cucsat.G14554 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q2V0Z54.6e-2840.54Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1[more]
BLAST of Cucsat.G14554 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004138204.31.76e-118100.00protein MIS12 homolog isoform X1 [Cucumis sativus][more]
KAA0058004.17.18e-10993.85Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote... [more]
KAA0058032.11.45e-10893.85Centromere protein Mis12 [Cucumis melo var. makuwa][more]
XP_038879945.17.31e-9784.92protein MIS12 homolog [Benincasa hispida][more]
KAG6589575.16.66e-9681.28Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of Cucsat.G14554 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPL29.94e-11899.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1[more]
A0A5A7US573.48e-10993.85Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7UQ957.01e-10993.85Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A6J1E0A13.06e-9480.21protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1[more]
A0A6J1JME31.76e-9380.21protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008685Centromere protein Mis12PFAMPF05859Mis12coord: 10..103
e-value: 1.8E-15
score: 57.3
IPR008685Centromere protein Mis12PANTHERPTHR14527PROTEIN MIS12 HOMOLOGcoord: 1..101

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G14554.T2Cucsat.G14554.T2mRNA
Cucsat.G14554.T3Cucsat.G14554.T3mRNA
Cucsat.G14554.T6Cucsat.G14554.T6mRNA
Cucsat.G14554.T12Cucsat.G14554.T12mRNA
Cucsat.G14554.T16Cucsat.G14554.T16mRNA
Cucsat.G14554.T15Cucsat.G14554.T15mRNA
Cucsat.G14554.T20Cucsat.G14554.T20mRNA
Cucsat.G14554.T21Cucsat.G14554.T21mRNA
Cucsat.G14554.T9Cucsat.G14554.T9mRNA
Cucsat.G14554.T1Cucsat.G14554.T1mRNA
Cucsat.G14554.T5Cucsat.G14554.T5mRNA
Cucsat.G14554.T4Cucsat.G14554.T4mRNA
Cucsat.G14554.T18Cucsat.G14554.T18mRNA
Cucsat.G14554.T19Cucsat.G14554.T19mRNA
Cucsat.G14554.T14Cucsat.G14554.T14mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051301 cell division
biological_process GO:0051382 kinetochore assembly
biological_process GO:0000070 mitotic sister chromatid segregation
biological_process GO:0034501 protein localization to kinetochore
biological_process GO:0000278 mitotic cell cycle
cellular_component GO:0000818 nuclear MIS12/MIND complex
cellular_component GO:0000775 chromosome, centromeric region
cellular_component GO:0005634 nucleus