Cucsat.G14431 (gene) Cucumber (B10) v3

BLAST of Cucsat.G14431 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 8.0e-222
Identity = 384/546 (70.33%), Postives = 466/546 (85.35%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDG+FRSLRPPLV PKD NLSM+SF+FRN  SYP + A++D++++ ++S+SQ 
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPKDHNLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K++ I+VS+G + LG++KNDVVLIFAPNS+   +CF+G++A GAI TT NP+YTVSEL+K
Sbjct: 61  KSMVIKVSHGFLNLGVQKNDVVLIFAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLL--DQQIPSAIHS-PKILCFNDLVNMAGDKS 180
           QV+D+ PKL+++V EL++KVK  N+PT+L+  + +  S + S  K+L F+DLV ++G   
Sbjct: 121 QVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRAKVLTFHDLVTLSGPV- 180

Query: 181 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLN 240
            S+FP+V  KQSDTAALLYSSGTTG SKGV+L+H NFIASSLM+TM+Q   GE H VFL 
Sbjct: 181 -SDFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLC 240

Query: 241 FLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 300
           FLPMFHVFGLA ITYAQLQ+GNTV+SM +F+LEK L  VEKYKVT LWVVPPV+LAL K 
Sbjct: 241 FLPMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKN 300

Query: 301 SLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGK 360
           S+VKKY+LSS+K +GSGAAPLG++LMEECA  +P  +V QGYGMTETCG+V++E+   GK
Sbjct: 301 SIVKKYDLSSLKHIGSGAAPLGKDLMEECAKIVPHGIVAQGYGMTETCGIVSVEDTRGGK 360

Query: 361 RNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHG 420
           R++GSAG L+SGVEA+IVSVDTLKPLPPNQ GEI VRGPNMM GYFNNP ATK TIDK G
Sbjct: 361 RHTGSAGMLSSGVEAQIVSVDTLKPLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKG 420

Query: 421 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEE 480
           WVHTGDLGYFDE+G LYVVDRIKELIKYKGFQVAPAELE LLVSHPEILDAVVIP PD +
Sbjct: 421 WVHTGDLGYFDEDGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAD 480

Query: 481 AGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIE 540
           AGEVP+AYVVRS NSSLTE+D+ KFIA QV+ +KRLR+VTFI+SVPKS SGKILRRELI+
Sbjct: 481 AGEVPVAYVVRSPNSSLTEDDVKKFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQ 540

Query: 541 KVRAKI 544
           KVR+ I
Sbjct: 541 KVRSNI 544

BLAST of Cucsat.G14431 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 1.9e-215
Identity = 374/547 (68.37%), Postives = 463/547 (84.64%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGI+RSLRP LV PKDPN S++SFLFRN  SYP++LAI D+++ +S+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   R+++G  +LGI KNDVVLIFAPNS QF +CF+ V AIG + TT NP+YTV+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQ----QIPSAIHSPKILCFNDLVNMAGDK 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL      +IP   +S KIL F++++ ++  +
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS-KILSFDNVMELS--E 180

Query: 181 SGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFL 240
             SE+P V +KQSDTAALLYSSGTTG SKGV LTHGNFIA+SLM+TMDQ   GE HGVFL
Sbjct: 181 PVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFL 240

Query: 241 NFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 300
            FLPMFHVFGLA ITY+QLQ+GN +VSM +F LE  L  +EK++VT LWVVPPV LAL+K
Sbjct: 241 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 300

Query: 301 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 360
           QS+VKK++LSS+K +GSGAAPLG++LMEEC  NIP+ +++QGYGMTETCG+V++E+P +G
Sbjct: 301 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 360

Query: 361 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 420
           KRNSGSAG LA GVEA+IVSV+T K  PPNQ GEI VRGPNMM GY NNP+ATK+TIDK 
Sbjct: 361 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 420

Query: 421 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 480
            WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP PDE
Sbjct: 421 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 480

Query: 481 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
           EAGEVPIA+VVRS NSS+TE+DI KFIA QV+PYKRLRRV+FIS VPKS +GKILRREL+
Sbjct: 481 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELV 540

Query: 541 EKVRAKI 544
           ++VR+K+
Sbjct: 541 QQVRSKM 544

BLAST of Cucsat.G14431 vs. ExPASy Swiss-Prot
Match: M4IQS1 (Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 5.1e-184
Identity = 328/544 (60.29%), Postives = 416/544 (76.47%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEK       I+ S RPP+ FP DP LS+ SFLFR+  SYPNR A++DA+S  ++++ +L
Sbjct: 1   MEKCFNPETQIYSSHRPPVNFPTDPKLSLTSFLFRSSASYPNRTALIDADSGQTLTFLKL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K    ++++ L+QL I+KNDVVLIFAPNS+ F +CF  + A+GAI TTCNP YT +EL+ 
Sbjct: 61  KTQVSKLAHSLVQLNIKKNDVVLIFAPNSIHFPVCFFSIAALGAITTTCNPSYTFTELSN 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           Q +D  P LVI+V ELW+K + LN+P ++L     S + S     F   +    D+  SE
Sbjct: 121 QAKDCNPTLVITVPELWEKARKLNLPAIILPSPSNSKLSSKSEFWFFSDLTRKSDRF-SE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
            PI  V+QSD AALLYSSGTTG SKGV+L+H NFI +SLM+T DQ   G+   + + FLP
Sbjct: 181 LPISDVRQSDVAALLYSSGTTGKSKGVVLSHKNFITTSLMVTSDQDRYGDPQNICMCFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS-L 300
           MFH+FGL+ I Y+QL++GN VVSM KF LE AL AVE Y+VT L+VVPPV++ALAK+S +
Sbjct: 241 MFHIFGLSVIAYSQLRRGNGVVSMGKFELEGALRAVEMYRVTHLFVVPPVMIALAKESVV 300

Query: 301 VKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRN 360
           V++Y+LSSVK + SGAAPLG+ +ME+CA N+P A +IQGYGMTETCG++++E+   G R 
Sbjct: 301 VRRYDLSSVKEILSGAAPLGKNVMEQCARNVPGAAIIQGYGMTETCGIISIEDSKKGCRF 360

Query: 361 SGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWV 420
           SGS G LA G+E++I+      PLPPNQ GEI +RGPNMM GY NN EATK TID+ GWV
Sbjct: 361 SGSTGMLAPGIESQIMDTKRWNPLPPNQEGEIWLRGPNMMQGYLNNLEATKSTIDEQGWV 420

Query: 421 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAG 480
            TGD+GYFDE GQL+VVDR+KELIK  GFQVAPAELEALL+SHPEILDAVVIP PDE+AG
Sbjct: 421 KTGDIGYFDEEGQLFVVDRLKELIKCYGFQVAPAELEALLLSHPEILDAVVIPFPDEKAG 480

Query: 481 EVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKV 540
           EVPIA VVRS NSSL+EED+ +FI  QV+P+K+LRRVTF+SSV KS +GKILRRELI+KV
Sbjct: 481 EVPIANVVRSPNSSLSEEDVQRFIEKQVAPFKKLRRVTFVSSVLKSPAGKILRRELIQKV 540

Query: 541 RAKI 544
           R+KI
Sbjct: 541 RSKI 543

BLAST of Cucsat.G14431 vs. ExPASy Swiss-Prot
Match: Q0DV32 (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 580.1 bits (1494), Expect = 2.6e-164
Identity = 302/542 (55.72%), Postives = 393/542 (72.51%), Query Frame = 0

Query: 4   SGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAES-SNSVSYSQLKA 63
           +GYG DG++RSLRPP     DP LS+   L R   + P+ +A+ DA +   ++++++L++
Sbjct: 9   AGYGADGVYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRS 68

Query: 64  LAIRVSNGL-IQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 123
             +  +  L  + G+   D VL+ APN V + +CF  V A+GA+ TT NP YT  E+ KQ
Sbjct: 69  AVLSTAVALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQ 128

Query: 124 VRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHS-PKILCFNDLVNMAGDKSGSE 183
           V DA+ KLVI+++ L  K+  L +P +LLD    +A  S P         N+      ++
Sbjct: 129 VSDARAKLVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEAD 188

Query: 184 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 243
           +    +KQSDTAALLYSSGTTG SKGVILTH NFIA++ M+T DQ    E   VFL FLP
Sbjct: 189 YRRPPIKQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLP 248

Query: 244 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 303
           MFH+FGL+ ITYAQL +GN +++M +F++   + AV++++VT L+ VPPV++ALAK    
Sbjct: 249 MFHIFGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKA 308

Query: 304 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGK-RN 363
            KY+LSS+K +GSGAAPLG+++ME  A   P + ++QGYGMTETCG+++LE P  G+ R 
Sbjct: 309 GKYDLSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQARE 368

Query: 364 SGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWV 423
            GS GTL SGVEAKIV + TLK LPPNQ GEI VRGPN+M GYFNN +AT+ TI K GW+
Sbjct: 369 FGSTGTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQGWL 428

Query: 424 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAG 483
           HTGDLGYFD  GQL+VVDR+KELIKYKGFQ+APAELE LL+SHPEILDAVVIP PD +AG
Sbjct: 429 HTGDLGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAKAG 488

Query: 484 EVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKV 542
           EVPIAYVVRS +SSLTE D+ KFI  QV+ YKRL+RVTF+ SVPKS SGKILRR+LI +V
Sbjct: 489 EVPIAYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLIAQV 548

BLAST of Cucsat.G14431 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 3.5e-124
Identity = 238/546 (43.59%), Postives = 362/546 (66.30%), Query Frame = 0

Query: 2   EKSGYGR-DGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 61
           E+SGY + + IF S R P+  P++ ++ + +F+  +  ++  ++A +DA +   +++ QL
Sbjct: 11  ERSGYCKSNSIFYSKREPVQLPQNHSVDVTTFI--SSRAHHGKIAFIDAATGRHLTFPQL 70

Query: 62  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 121
                 V+  L  +GI K DV+L+ +PNS+ F +  + V+++GAI+TT NP+ T  E+ K
Sbjct: 71  WRAVDSVATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAK 130

Query: 122 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 181
           Q+ D+KP L  ++ +L  K+   N+P V++D ++ S++    +   + L  M   +    
Sbjct: 131 QITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEK-TLNIVSSLGEMMRKEPSPN 190

Query: 182 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEE-HGVFLNFL 241
                V Q DTA LLYSSGTTGASKGV+ +H N IA  ++ T+   F  E+    F+  +
Sbjct: 191 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIA--MVQTILSRFGTEDGEHTFICTV 250

Query: 242 PMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS- 301
           PMFH++GLA      L  G+T+V + KF + + L A+EKY+ T L +VPP+++AL K + 
Sbjct: 251 PMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNAN 310

Query: 302 -LVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGK 361
            +  KY+LSS++ V SG APL +E++E    N P+  ++QGYG+TE+ G+ A  +     
Sbjct: 311 HIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQES 370

Query: 362 RNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHG 421
           R  G+AG L+  +EAKIV+ +T + L  N+ GE+ +RGP +M GYF+N EAT  TID  G
Sbjct: 371 RRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEG 430

Query: 422 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEE 481
           W+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL+SHPEI DA VIP PD+E
Sbjct: 431 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKE 490

Query: 482 AGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIE 541
           AG+ P+AYVVR   S+L+E  ++ FIA  V+PYKR+R+V F++S+PK+ SGKILR++LI+
Sbjct: 491 AGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIK 550

Query: 542 KVRAKI 544
              +K+
Sbjct: 551 LATSKL 551

BLAST of Cucsat.G14431 vs. NCBI nr
Match: XP_004147290.1 (4-coumarate--CoA ligase-like 7 [Cucumis sativus] >KGN64806.1 hypothetical protein Csa_013302 [Cucumis sativus])

HSP 1 Score: 1063 bits (2749), Expect = 0.0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsat.G14431 vs. NCBI nr
Match: XP_008463063.1 (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo] >KAA0051818.1 4-coumarate--CoA ligase-like 7 [Cucumis melo var. makuwa] >TYK21426.1 4-coumarate--CoA ligase-like 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1031 bits (2665), Expect = 0.0
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsat.G14431 vs. NCBI nr
Match: XP_038895538.1 (4-coumarate--CoA ligase-like 7 [Benincasa hispida])

HSP 1 Score: 1003 bits (2594), Expect = 0.0
Identity = 510/543 (93.92%), Postives = 529/543 (97.42%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPK PNLSMISFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKHPNLSMISFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA  IRVSNGL+QLGIEKNDVVLIFAPNSVQ++ICF+G+IAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KASVIRVSNGLLQLGIEKNDVVLIFAPNSVQYSICFLGIIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQQIPSAIHS K+LCFNDL+NMAGDKSGS+
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQQIPSAIHSSKLLCFNDLMNMAGDKSGSD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIV VKQSDTAALLYSSGTTGASKGVILTH NFI SSLMITMDQTF G+E+GV+LNFLP
Sbjct: 181 FPIVEVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITMDQTFRGKEYGVYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+KRVGSGAAPLG+ELMEEC NNIPSAVV+QGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSLKRVGSGAAPLGKELMEECGNNIPSAVVLQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDE GQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD EAGE
Sbjct: 421 TGDLGYFDEYGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDAEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsat.G14431 vs. NCBI nr
Match: KAG6607161.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036848.1 4-coumarate--CoA ligase-like 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 966 bits (2498), Expect = 0.0
Identity = 483/543 (88.95%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsat.G14431 vs. NCBI nr
Match: XP_022949355.1 (4-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate--CoA ligase-like 7 [Cucurbita moschata])

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 482/543 (88.77%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AK+
Sbjct: 541 AKL 543

BLAST of Cucsat.G14431 vs. ExPASy TrEMBL
Match: A0A0A0LXU2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1)

HSP 1 Score: 1063 bits (2749), Expect = 0.0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsat.G14431 vs. ExPASy TrEMBL
Match: A0A5D3DCX7 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold609G00860 PE=4 SV=1)

HSP 1 Score: 1031 bits (2665), Expect = 0.0
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsat.G14431 vs. ExPASy TrEMBL
Match: A0A1S3CIB0 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1)

HSP 1 Score: 1031 bits (2665), Expect = 0.0
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsat.G14431 vs. ExPASy TrEMBL
Match: A0A6J1GCI6 (4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111452734 PE=4 SV=1)

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 482/543 (88.77%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AK+
Sbjct: 541 AKL 543

BLAST of Cucsat.G14431 vs. ExPASy TrEMBL
Match: A0A6J1KDQ6 (4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111493400 PE=4 SV=1)

HSP 1 Score: 962 bits (2486), Expect = 0.0
Identity = 480/543 (88.40%), Postives = 519/543 (95.58%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP DPNLS+ SFLFRN  SYPNRLAIVDAESS+S SYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPTDPNLSVASFLFRNSSSYPNRLAIVDAESSDSASYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVYTVSEL+K
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLD+QIP  I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDKQIPLEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGV+LTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVVLTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN AVGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRAVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 543
           AKI
Sbjct: 541 AKI 543