Cucsat.G14380 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G14380
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	E3 ubiquitin-protein ligase RMA1H1-like
Location	ctg1869: 6782611 .. 6785488 (-)
RNA-Seq Expression	Cucsat.G14380
Synteny	Cucsat.G14380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCCGCCTTACTCATTTTCCACTTTCTCGCTTACATAATTTCATGGTAAAACATTTTTGTTCAAGCTCCTCAAATTCACACCTCTCGGTAACTGAGCCTTTCTTTCTTTCTTCTTTAACACTTCAAGCAGCTTTCCTCTGATAATTTTCCTGGAAACTGATTTTTCTTTTTCTTCACCATCTTTCAATTTCGTTCAATTATTCATCGTAGGTTTCGTTTCCGATTTCCCCCTTATTGAGAAACTTAAATTTGTTGTGTCTCTCTTTGTGAAATGCATTATTTCGATTTATTATTCAGAGTTGGAACTGTTTTGGGATTGAAATCCGTGGCCGTTCTTTACTTTTTTTTTTTTTTTTCTGTTCGTTCTGTTTGGATTTGGTTTTCAGTTCATCGATCTGTGTGGATTAACATTCTTAATAGCGATAAACATCGGCCGCTCTTGTTCACTTATATGAAGAAATCAATCCTCTGTTTTTTACTCTTTTCAGAAATTCGTTTTTAACTTTGAAGATCTCGTTTGATATTATGAATGGTGATTCTTAAATTCTAGATGTTCACATTGACATGATTGGATGAAGAAATATCAAGTTCCTTTTTCTTTTTTCTTTTAGTACTTTAGCATCTTCAAACTAGATTCTTTTTCGTATTTCATCCGAAGATTTTCTAGCTTCTACTTAACATTTCTCTTTTTCCATAAAGTAATTAATTTTAACATTGGCATCAATAAAAAAAACTCAACTTGACAATTCTCTCATTTTAAGAAAAGAAAAAAGAATACAGAACCTTGACATTTCTCTTGTTCATTTAACAAATGTATAAGAAATGGGGTTTGGGGGGTTTTGAAGTCAAAGGTTTGATAACTAATACCGAGATCAATTCAAAATTTTGTTGAATTGATTTTTAGAAATTTTAGAGTTTGAATTATAGTTAGGATTTAGTGTTTGTGTTTGATAAGATCATCACCGTTTATGAGCTTTTTATCAGGCCATAATGAATATTTTTCTAAGTTTAATCAAATTAATACTAACATTTAAAACTATAAAGATTAAATTTGTTTGGTCTCAAAAGAGAGTGGAACGAGAAACTTTTGAACATAGAGTTTGGAGGTTTCACGATTAGAAAATTCCAAATTCACTTAGGGAACAAATTTGTAATTTAAACAATTTTTTATTAAATGTGTGGCAGAAAAAAATACAATTTTTTTTAGAAGAAAGTTTGAAATTTACAAGGTAAGAGATCAAAATAGATTAATTAAAAACTTAAGAAGTAAGTTAGGTTTAAACTTATTTTTGAAATTATTTCCACTAATTCTAAGATTTTTCAGACTAGTATATATTGAACTTTTAGTCAGATAATTGTACATTAATAAATCATAACCAAATTAGTTCTAATTATTGTCTAGAATTGGATGGATATACCCTTAACTTCATAAAAGCCACAAACTTTTGATCACATTAGTATCTGCCTAAGATTTTTGTGGTTTTGTACCACTTGGCTTGACAATCTAACCACATTTAACAACTTTGGGCCACGACAAAGACAAGACTGTAGGCTGTAAGAATGAACAGTTCCTTGGCACATTATGAGTCGTAAGAAATTGTAACACATTTTTCTTATTTTTTGACCTTTCCTCTGTTGAAGTTTCTCATCAAAACGCTTTCAATTAGTTATAGATCAAGACATGTTTCACATTTATTCTTCCAAATCTTATGTGAAGTTATCCATTTCAGGTCAAATCCTAGTGTGAAGCTAATTTGTAGAAGGCTTATAGAGATAACATGGGCTTTGAGCACTACTTTTCCCATGGATGGAGGAAGCAAATCCCAGCTTCAATGGAAAATTCTGAGAAGTTTAAGCCTTGTTTTGATTGCAACATTTGCTTAGACTTCGCATCTGACCCTATAGTTACCCTTTGTGGCCACCTCTATTGCTGGCCTTGCATTTACAAGTGGCTTCACGTCCAGAGCGCTTCCCTCGCCCCCGACGAGCCTCCGCAGTGTCCGGTTTGCAAGGCCAATATTTCCCACACGACAATGGTCCCTCTCTACGGCCGTGGTCAATCGGCTGAACATGCTGAGGTTGATGCCAGAGGGATGCTTATACCTCCTAGACCATCAGCTTTTGGCAACAAGGCTCTAGCAACTATCAGATCTAATAATGCTTCTAGCCAGCAGTTTGCTTATGGAGACCTAGATCAAAACCAAAACTACAACTTTGATGTGTATGATAGCTATGAAGAGGACTCAGGCAGCTCGTTGTTTAGCCTCGAAGACAACCCGGTTGGAAGCTCTCATCATCCTACGGCCGGGATGCTCGGAGAGACAGTTTATGGTAGGTTCTATTGGGACTCAGAGAACATATACCGACACCCGAATTCTTATGGTTTAAGTGGGAGTAATAGTCCTCGGTTGAGAAGGCAGGAAATAGTGGCTGAGAAATCGCTTAACAAAATATATTTTTTTCTTTTCTGTTGCTTTCTTTTGTGTCTTGCTGTATTCTAATTACAATTGGAGATTTGTGTAGGCTCTCATTTGTAACCAGTTTTGTAAAGGGATATGAAACTTTGGTACCATAGATTAATTAAACACACCACATTTTGTGTAGCCTTTGATTCCTTTCCAGTAAGGAACTGCCGGTGAGAGGTACTTCACCAGTTATTCAATTTCAAAAGCAAAATATTAGGTTCATGGTACAAACTTATTTACTATTACAAAAATGACAGAAAAAACAAAATTATTTACTATTATAAAAATGACAGAAAAGTTAAATTTGGCAGATTCCGTCATCATCGCTCTACCAATATATCATGAGCACGAGCAGGATGAGAGTCATCTTTTTCCCTCAACAAATTAACCACTTTCTCAGTTTCTTCCTCATC

Coding sequence (CDS)

ATGGGCTTTGAGCACTACTTTTCCCATGGATGGAGGAAGCAAATCCCAGCTTCAATGGAAAATTCTGAGAAGTTTAAGCCTTGTTTTGATTGCAACATTTGCTTAGACTTCGCATCTGACCCTATAGTTACCCTTTGTGGCCACCTCTATTGCTGGCCTTGCATTTACAAGTGGCTTCACGTCCAGAGCGCTTCCCTCGCCCCCGACGAGCCTCCGCAGTGTCCGGTTTGCAAGGCCAATATTTCCCACACGACAATGGTCCCTCTCTACGGCCGTGGTCAATCGGCTGAACATGCTGAGGTTGATGCCAGAGGGATGCTTATACCTCCTAGACCATCAGCTTTTGGCAACAAGGCTCTAGCAACTATCAGATCTAATAATGCTTCTAGCCAGCAGTTTGCTTATGGAGACCTAGATCAAAACCAAAACTACAACTTTGATGTGTATGATAGCTATGAAGAGGACTCAGGCAGCTCGTTGTTTAGCCTCGAAGACAACCCGGTTGGAAGCTCTCATCATCCTACGGCCGGGATGCTCGGAGAGACAGTTTATGGTAGGTTCTATTGGGACTCAGAGAACATATACCGACACCCGAATTCTTATGGTTTAAGTGGGAGTAATAGTCCTCGGTTGAGAAGGCAGGAAATAGTGGCTGAGAAATCGCTTAACAAAATATATTTTTTTCTTTTCTGTTGCTTTCTTTTGTGTCTTGCTGTATTCTAA

Protein sequence

MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLHVQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKALATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLGETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF

Homology

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BLAST of Cucsat.G14380 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 3.6e-41
Identity = 99/231 (42.86%), Postives = 133/231 (57.58%), Query Frame = 0


Query: 20  ENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLHVQSASL--APDEPPQCPVC 79
           E  E     FDCNICLD   +P++TLCGHLYCWPCIYKW++ QS S   +  + PQCPVC
Sbjct: 30  EVEENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQPQCPVC 89

Query: 80  KANISHTTMVPLYGR-GQSAEHAEVDARGMLI-----PPRPSAFGNKALATIRSNNASSQ 139
           KA +S  T++PLYGR GQS + +E  A  + I     PP P   G+  L T  SN +   
Sbjct: 90  KAEVSEKTLIPLYGRGGQSTKPSEGKAPNLGIVIPQRPPSPRCGGHFLLPTTDSNPS--- 149

Query: 140 QFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTA-GMLGETVYGRFYWD 199
                 L Q + Y            GS + S   +P G++ +     M+GE  Y R + +
Sbjct: 150 -----QLLQRRGYQQQSQTRQPAYQGSYMSSPMLSPGGATANMLQHSMIGEVAYARIFGN 209

Query: 200 SE-NIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 241
           S   +Y +PNSY L+ S+SPR+RRQ   A++SL +I FFLFCCF+ CL +F
Sbjct: 210 SSTTMYTYPNSYNLAISSSPRMRRQLSQADRSLGRICFFLFCCFVTCLILF 252

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BLAST of Cucsat.G14380 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 6.6e-35
Identity = 86/230 (37.39%), Postives = 129/230 (56.09%), Query Frame = 0


Query: 15  IPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLHVQSASLAPD--EPP 74
           I  S+ + +     FDCNICLD   +P+VTLCGHL+CWPCI+KWL VQS S + +     
Sbjct: 32  ITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHR 91

Query: 75  QCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKALATIRSNNASSQQ 134
           QCPVCK+ +SH+T+VPLYGRG+     E       +P RP       +  +      +  
Sbjct: 92  QCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS---VPKRP-------VGPVYRLEMPNSP 151

Query: 135 FAYGDLDQNQNYNFD-VYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLGETVYGRFYWDS 194
           +A  DL  +Q  +F+   + Y   SG  + S       +   P   M+GE V  R +   
Sbjct: 152 YASTDLRLSQRVHFNSPQEGYYPVSG--VMSSNSLSYSAVLDPVMVMVGEMVATRLFGTR 211

Query: 195 -ENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 241
             + + +P++Y L+G++ PR+RR+ + A+KSL +I+FF  CC +LCL +F
Sbjct: 212 VMDRFAYPDTYNLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

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BLAST of Cucsat.G14380 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.6e-33
Identity = 90/221 (40.72%), Postives = 120/221 (54.30%), Query Frame = 0


Query: 28  CFDCNICLDFASDPIVTLCGHLYCWPCIYKWLHVQSASLAPDE-PPQCPVCKANISHTTM 87
           CFDCNICLD A DP+VTLCGHL+CWPCIYKWLHVQ +S++ D+    CPVCK+NI+ T++
Sbjct: 41  CFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSL 100

Query: 88  VPLYGRGQSAEHA-----EVDARGMLIP--PRPSAFGNKALATIRSNNASSQQFAYGDLD 147
           VPLYGRG S+  +     + DA    IP  P PSA  N  + +  S N S Q        
Sbjct: 101 VPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRN-PITSASSLNPSLQHQTLSPSF 160

Query: 148 QNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLGETVYGRFYWDSENIYRHPN 207
            N  Y+   + + E         L +  + S  +P  GM G+ VY R +    N    P 
Sbjct: 161 HNHQYSPRGFTTTEST------DLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIAQP- 220

Query: 208 SYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 241
                   S R+ ++    EKSLN++  F  CC +LCL +F
Sbjct: 221 ------YQSQRMMQR----EKSLNRVSIFFLCCIILCLLLF 243

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BLAST of Cucsat.G14380 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 8.6e-27
Identity = 76/222 (34.23%), Postives = 109/222 (49.10%), Query Frame = 0


Query: 29  FDCNICLDFASDPIVTLCGHLYCWPCIYKWLHVQSASLA-------PDEPPQCPVCKANI 88
           FDCNICLD   DP+VTLCGHL+CWPCI+KW +  + S           EPP+CPVCK+++
Sbjct: 19  FDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKSDV 78

Query: 89  SHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKALATIRSNNASSQQFAYGDLDQN 148
           S  T+VP+YGRGQ A  +     G  +P RP+         +       Q+   G   ++
Sbjct: 79  SEATLVPIYGRGQKAPQS-----GSNVPSRPT-------GPVYDLRGVGQRLGEG---ES 138

Query: 149 QNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLGETVYGRFYWDSEN---IYRHP 208
           Q Y + + D                       P  G++ E VY R + +S +    YR  
Sbjct: 139 QRYMYRMPD-----------------------PVMGVVCEMVYRRLFGESSSNMAPYRDM 193

Query: 209 NSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 241
           N          R RR+ + AE+SL+++Y FL C   +CL +F
Sbjct: 199 N---------VRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

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BLAST of Cucsat.G14380 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.8e-17
Identity = 45/109 (41.28%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 29  FDCNICLDFASDPIVTLCGHLYCWPCIYKWLHVQSASLAPDEPPQCPVCKANISHTTMVP 88
           F+CNICLD A D +++LCGHL+CWPC+++WL  +           CPVCKA IS   ++P
Sbjct: 39  FECNICLDTAKDAVISLCGHLFCWPCLHQWLETR------PNRQVCPVCKAGISRDKVIP 98

Query: 89  LYGRGQSAEHAEVDARGMLIPPRPSAFGNKALATIRSNNASSQQFAYGD 138
           LYGRG + +    D R    PPRP     +       N    Q F +GD
Sbjct: 99  LYGRGSTGQQ---DPREK-TPPRPQGQRPEP-----ENRGGFQGFGFGD 132

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BLAST of Cucsat.G14380 vs. NCBI nr
Match: XP_011653163.1 (E3 ubiquitin-protein ligase RMA1H1 [Cucumis sativus] >KGN64674.1 hypothetical protein Csa_013288 [Cucumis sativus])

HSP 1 Score: 511 bits (1316), Expect = 3.41e-183
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH 60
           MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH
Sbjct: 1   MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL 120
           VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL
Sbjct: 61  VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL 120

Query: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG 180
           ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG
Sbjct: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG 180

Query: 181 ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240
           ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF
Sbjct: 181 ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240

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BLAST of Cucsat.G14380 vs. NCBI nr
Match: XP_008442466.1 (PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >XP_008442474.1 PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >XP_008442488.1 PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0056985.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa] >TYK26413.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa])

HSP 1 Score: 481 bits (1238), Expect = 2.67e-171
Identity = 226/240 (94.17%), Postives = 232/240 (96.67%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH 60
           M FEHYFSHGWRKQIPASM NSEKFKPCFDCNICLDFAS+PIVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEHYFSHGWRKQIPASMANSEKFKPCFDCNICLDFASEPIVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL 120
           VQSASLAPDEPPQCPVCKA+ISHTTMVPLYGRGQSAEHAE DARG LIPPRPSAFGNKAL
Sbjct: 61  VQSASLAPDEPPQCPVCKADISHTTMVPLYGRGQSAEHAEDDARGTLIPPRPSAFGNKAL 120

Query: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG 180
           ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAG+LG
Sbjct: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGLLG 180

Query: 181 ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240
            TVYGR Y DSEN+YRHPNSYGLSGSNSPRLRRQEIVAEKSLN+I FFLFCCF+LCLAVF
Sbjct: 181 GTVYGRLYGDSENVYRHPNSYGLSGSNSPRLRRQEIVAEKSLNRISFFLFCCFILCLAVF 240

[Close]

BLAST of Cucsat.G14380 vs. NCBI nr
Match: XP_038895713.1 (E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida])

HSP 1 Score: 435 bits (1118), Expect = 5.25e-153
Identity = 202/240 (84.17%), Postives = 219/240 (91.25%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH 60
           M FEHYF HGWR+Q+PA+M NSEKFKPCFDCNICLDFAS+PIVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEHYFPHGWRRQVPAAMANSEKFKPCFDCNICLDFASEPIVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL 120
           VQSASL PDEPPQCPVCKANISHTTMVPLYGRGQSAE AEVDARG+LIPPRPSAFGNKAL
Sbjct: 61  VQSASLTPDEPPQCPVCKANISHTTMVPLYGRGQSAEDAEVDARGLLIPPRPSAFGNKAL 120

Query: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG 180
           ATIRSN AS QQFAYGDL QNQ YNFD+YDSYEEDS SSLF+LED+ VGS HHPTAGM  
Sbjct: 121 ATIRSNTASGQQFAYGDLYQNQIYNFDMYDSYEEDSSSSLFNLEDSSVGSLHHPTAGMFT 180

Query: 181 ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240
           ETVY RF+ +SEN+Y HP+SYG SG++SPRLRRQEI+AEKSLN+I  FLFCCF+LCL VF
Sbjct: 181 ETVYARFFGNSENVYTHPSSYGPSGNSSPRLRRQEILAEKSLNRISIFLFCCFVLCLVVF 240

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BLAST of Cucsat.G14380 vs. NCBI nr
Match: XP_023519102.1 (E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_023519103.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_023519104.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 395 bits (1014), Expect = 3.32e-137
Identity = 188/241 (78.01%), Postives = 207/241 (85.89%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPAS-MENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWL 60
           M FEHYFS  WR+ +PAS MENSEKFK CFDCNICL+FAS+PIVTLCGHLYCWPCIYKWL
Sbjct: 1   MAFEHYFSRRWRRCVPASSMENSEKFKACFDCNICLEFASEPIVTLCGHLYCWPCIYKWL 60

Query: 61  HVQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKA 120
           HVQSASL PDEPPQCPVCKA+ISHTTMVPLYGRGQSA+ AEVD RG+LIPPRPSAFGNK 
Sbjct: 61  HVQSASLLPDEPPQCPVCKADISHTTMVPLYGRGQSAKEAEVDPRGLLIPPRPSAFGNKV 120

Query: 121 LATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGML 180
           LA+I SNNAS +Q  YGDL QNQN NFD+YDSYEEDS SSLF++EDNPVGS  HPTA M 
Sbjct: 121 LASIGSNNASGEQLVYGDLYQNQNDNFDMYDSYEEDSSSSLFNMEDNPVGSFGHPTARMF 180

Query: 181 GETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAV 240
           GETVYGR +  SEN+Y HPNSY L+GS SP+LR QE    KSLN+I  FLFCCF+LCLA+
Sbjct: 181 GETVYGRLFGSSENLYAHPNSYALTGSGSPQLRMQE----KSLNRISIFLFCCFVLCLAI 237

[Close]

BLAST of Cucsat.G14380 vs. NCBI nr
Match: XP_022926997.1 (E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata] >XP_022926998.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata] >XP_022926999.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata])

HSP 1 Score: 392 bits (1006), Expect = 5.49e-136
Identity = 186/241 (77.18%), Postives = 206/241 (85.48%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPAS-MENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWL 60
           M FEHYFS  WR+ +PAS MENSEKFK CFDCNICL+FAS+PIVTLCGHLYCWPCIYKWL
Sbjct: 1   MAFEHYFSRRWRRCVPASSMENSEKFKACFDCNICLEFASEPIVTLCGHLYCWPCIYKWL 60

Query: 61  HVQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKA 120
           H+QSASL PDEPPQCPVCKA+ISHTTMVPLYGR QSA+ AEVD RG+LIPPRPSAFGNK 
Sbjct: 61  HIQSASLLPDEPPQCPVCKADISHTTMVPLYGRDQSAKEAEVDPRGLLIPPRPSAFGNKV 120

Query: 121 LATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGML 180
           LA+I SNNAS +Q  YGDL QNQN NFD+YDSYEEDS SSLF++EDNPVGS  HPTA M 
Sbjct: 121 LASIGSNNASGEQLVYGDLYQNQNDNFDMYDSYEEDSSSSLFNMEDNPVGSFGHPTARMF 180

Query: 181 GETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAV 240
           GETVYGR +  SEN+Y HPNSY L+GS SP+LR QE    KSLN+I  FLFCCF+LCLA+
Sbjct: 181 GETVYGRLFGSSENLYAHPNSYALTGSGSPQLRMQE----KSLNRISIFLFCCFVLCLAI 237

[Close]

BLAST of Cucsat.G14380 vs. ExPASy TrEMBL
Match: A0A0A0LUK5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073810 PE=4 SV=1)

HSP 1 Score: 511 bits (1316), Expect = 1.65e-183
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH 60
           MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH
Sbjct: 1   MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL 120
           VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL
Sbjct: 61  VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL 120

Query: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG 180
           ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG
Sbjct: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG 180

Query: 181 ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240
           ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF
Sbjct: 181 ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240

[Close]

BLAST of Cucsat.G14380 vs. ExPASy TrEMBL
Match: A0A1S3B6D8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103486320 PE=4 SV=1)

HSP 1 Score: 481 bits (1238), Expect = 1.29e-171
Identity = 226/240 (94.17%), Postives = 232/240 (96.67%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH 60
           M FEHYFSHGWRKQIPASM NSEKFKPCFDCNICLDFAS+PIVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEHYFSHGWRKQIPASMANSEKFKPCFDCNICLDFASEPIVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL 120
           VQSASLAPDEPPQCPVCKA+ISHTTMVPLYGRGQSAEHAE DARG LIPPRPSAFGNKAL
Sbjct: 61  VQSASLAPDEPPQCPVCKADISHTTMVPLYGRGQSAEHAEDDARGTLIPPRPSAFGNKAL 120

Query: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG 180
           ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAG+LG
Sbjct: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGLLG 180

Query: 181 ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240
            TVYGR Y DSEN+YRHPNSYGLSGSNSPRLRRQEIVAEKSLN+I FFLFCCF+LCLAVF
Sbjct: 181 GTVYGRLYGDSENVYRHPNSYGLSGSNSPRLRRQEIVAEKSLNRISFFLFCCFILCLAVF 240

[Close]

BLAST of Cucsat.G14380 vs. ExPASy TrEMBL
Match: A0A5A7UTV8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00770 PE=4 SV=1)

HSP 1 Score: 481 bits (1238), Expect = 1.29e-171
Identity = 226/240 (94.17%), Postives = 232/240 (96.67%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPASMENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWLH 60
           M FEHYFSHGWRKQIPASM NSEKFKPCFDCNICLDFAS+PIVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEHYFSHGWRKQIPASMANSEKFKPCFDCNICLDFASEPIVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKAL 120
           VQSASLAPDEPPQCPVCKA+ISHTTMVPLYGRGQSAEHAE DARG LIPPRPSAFGNKAL
Sbjct: 61  VQSASLAPDEPPQCPVCKADISHTTMVPLYGRGQSAEHAEDDARGTLIPPRPSAFGNKAL 120

Query: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGMLG 180
           ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAG+LG
Sbjct: 121 ATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGLLG 180

Query: 181 ETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAVF 240
            TVYGR Y DSEN+YRHPNSYGLSGSNSPRLRRQEIVAEKSLN+I FFLFCCF+LCLAVF
Sbjct: 181 GTVYGRLYGDSENVYRHPNSYGLSGSNSPRLRRQEIVAEKSLNRISFFLFCCFILCLAVF 240

[Close]

BLAST of Cucsat.G14380 vs. ExPASy TrEMBL
Match: A0A6J1EJS4 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC111433961 PE=4 SV=1)

HSP 1 Score: 392 bits (1006), Expect = 2.66e-136
Identity = 186/241 (77.18%), Postives = 206/241 (85.48%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPAS-MENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWL 60
           M FEHYFS  WR+ +PAS MENSEKFK CFDCNICL+FAS+PIVTLCGHLYCWPCIYKWL
Sbjct: 1   MAFEHYFSRRWRRCVPASSMENSEKFKACFDCNICLEFASEPIVTLCGHLYCWPCIYKWL 60

Query: 61  HVQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKA 120
           H+QSASL PDEPPQCPVCKA+ISHTTMVPLYGR QSA+ AEVD RG+LIPPRPSAFGNK 
Sbjct: 61  HIQSASLLPDEPPQCPVCKADISHTTMVPLYGRDQSAKEAEVDPRGLLIPPRPSAFGNKV 120

Query: 121 LATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGML 180
           LA+I SNNAS +Q  YGDL QNQN NFD+YDSYEEDS SSLF++EDNPVGS  HPTA M 
Sbjct: 121 LASIGSNNASGEQLVYGDLYQNQNDNFDMYDSYEEDSSSSLFNMEDNPVGSFGHPTARMF 180

Query: 181 GETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAV 240
           GETVYGR +  SEN+Y HPNSY L+GS SP+LR QE    KSLN+I  FLFCCF+LCLA+
Sbjct: 181 GETVYGRLFGSSENLYAHPNSYALTGSGSPQLRMQE----KSLNRISIFLFCCFVLCLAI 237

[Close]

BLAST of Cucsat.G14380 vs. ExPASy TrEMBL
Match: A0A6J1KJG8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita maxima OX=3661 GN=LOC111495790 PE=4 SV=1)

HSP 1 Score: 379 bits (972), Expect = 4.02e-131
Identity = 182/241 (75.52%), Postives = 203/241 (84.23%), Query Frame = 0


Query: 1   MGFEHYFSHGWRKQIPAS-MENSEKFKPCFDCNICLDFASDPIVTLCGHLYCWPCIYKWL 60
           M FEHYFS  WR+ +PAS MENSE+FK CFDCNICL+FAS+PIVTLCGHLYCWPCIYKWL
Sbjct: 1   MAFEHYFSRRWRRCVPASSMENSEQFKACFDCNICLEFASEPIVTLCGHLYCWPCIYKWL 60

Query: 61  HVQSASLAPDEPPQCPVCKANISHTTMVPLYGRGQSAEHAEVDARGMLIPPRPSAFGNKA 120
           HVQS SL PDEPPQCPVCKA+ISHTTMVPLYGRGQSA+ AEVD RG+LIPPRPSAFGNK 
Sbjct: 61  HVQSDSLLPDEPPQCPVCKADISHTTMVPLYGRGQSAKEAEVDPRGLLIPPRPSAFGNKV 120

Query: 121 LATIRSNNASSQQFAYGDLDQNQNYNFDVYDSYEEDSGSSLFSLEDNPVGSSHHPTAGML 180
           LA+I SNNAS +Q  Y DL Q QN NFD+YDSYEEDS SSLF+LEDNPVGS  HPTA M 
Sbjct: 121 LASIGSNNASGEQLVYRDLYQYQNDNFDMYDSYEEDSSSSLFNLEDNPVGSFGHPTARMF 180

Query: 181 GETVYGRFYWDSENIYRHPNSYGLSGSNSPRLRRQEIVAEKSLNKIYFFLFCCFLLCLAV 240
           GETVY R +  SE+++ HPNSY L+GS SP+LR QE    KSLN+I  FLFCCF+LCLA+
Sbjct: 181 GETVYARLFGSSESLHAHPNSYALTGSGSPQLRIQE----KSLNRISIFLFCCFVLCLAI 237

The following BLAST results are available for this feature:

BLAST of Cucsat.G14380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6R567	3.6e-41	42.86	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
O64425	6.6e-35	37.39	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
Q8GUK7	1.6e-33	40.72	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
P93030	8.6e-27	34.23	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q5ZIR9	2.8e-17	41.28	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]

BLAST of Cucsat.G14380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011653163.1	3.41e-183	100.00	E3 ubiquitin-protein ligase RMA1H1 [Cucumis sativus] >KGN64674.1 hypothetical pr...	[more]
XP_008442466.1	2.67e-171	94.17	PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >XP_008442474....	[more]
XP_038895713.1	5.25e-153	84.17	E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida]	[more]
XP_023519102.1	3.32e-137	78.01	E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_0235191...	[more]
XP_022926997.1	5.49e-136	77.18	E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata] >XP_022926998.1 E3 ...	[more]

BLAST of Cucsat.G14380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUK5	1.65e-183	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073810 P...	[more]
A0A1S3B6D8	1.29e-171	94.17	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103486320 ...	[more]
A0A5A7UTV8	1.29e-171	94.17	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1EJS4	2.66e-136	77.18	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KJG8	4.02e-131	75.52	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita maxima OX=3661 GN=LOC111495...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 31..77 e-value: 5.4E-9 score: 45.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 31..78 score: 12.386007
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 31..77 e-value: 1.7E-8 score: 34.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 10..133 e-value: 3.0E-17 score: 64.5
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 3..240
None	No IPR available	PANTHER	PTHR12313:SF6	E3 UBIQUITIN-PROTEIN LIGASE RMA3	coord: 3..240
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 29..79 e-value: 2.87471E-27 score: 96.9895
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 24..90
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 46..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G14380.T1	Cucsat.G14380.T1	mRNA
Cucsat.G14380.T2	Cucsat.G14380.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016020	membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity