Cucsat.G14348 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G14348
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Terpene_synth domain-containing protein
Location	ctg1869: 6254886 .. 6258776 (-)
RNA-Seq Expression	Cucsat.G14348
Synteny	Cucsat.G14348

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGACCAACACTGTTTAATTCACTCATATTATGGTTTATCTCCCGATAATAGACTCTTTATCATTTATGATTTATTAGTGGTAAATCAATATTTTACAACATAGACTATCAATAATAGAATTGGATAGATTTTGCTATATTTACAAATTTTAAAAACAAGTTGATATATGCTCTATTTGTAACTATCCTTAATAAGTTTGATAGTCTATAAAAATGCATAGAGAGCGAGAAGGTCGGCACACACTCTTATCATTGTAGCAATACACAGGTCAAACACAAGAACGAGAAGAATATTTGAAATTTAGTAGCAAAATCAATATTCTCCTTTTTAATTTTGTAGCCTTTTAATATTCCCATTACGGCCCAACTCATGTATCTTCAATCCTTTCTTGCTTCTCCCTGTCCAAAAATATTCATTTATCAAACAAAAAATCCACACAAAGTTCAGAATAACAACGCATGGCTTCAATTGAATGACCCTCTTGAAAAATCTTTTGCCAGAAAAGGGTGCATTCATGCCTTGAATTCTTCTTCATCATCCAATAAGTGCATCATCTCCAATTTGAAAATTGTATATTATCTTATTATTTTGGTCCATTGATTTTGTCTCCTTATGATTTCTCCTTTCATTTATTTATTTATTATTTTTTATGATGAGTCTTATAAATGATGATGTGTATAGGAGGAGTTTGGGGTTGAAACTCCAGCGAGAAAAGTGGAGATTTTAAAGGACATACTAACAGAAACAAGAGATGATCTGTGTTGGGAACGTTTGGAAATAATTGATGCAGCCCAATGGCTTGGCATTGACAACCAGTTCGAAGAAGAGATTGAAGATGTTCTTAAGAGGCAATATGATTTGATAAATGCATATCGTTTTGATGGAGACATGGATCTTCACAAAGCTGCCCTCCTTTTTCGACTTTTGAGACAACAACGATACCTTATATCTCAAGGTTCATATTCCACACTCTTTACCAACGAATTAAGCTTGAAGGTCTATAAGAAACATGGTTGGAATCCCATTGGTTCCTTGAGAAAAACGGTATTTTTTGTTAGATCCAAATTTAGCATTTTGGAAACCAAACGGATTAACAAATGCTATATGACAGGGAAGTACTTACAAAAACATTAACAAAAATGACAGACAATATAGCCCTTGATTCTGTCTTTATTATATGAGATTCAGTGCCATAGAAAACCCACTATTTGCACAATTTTTTTTCCTTCAAACGGTTGTTACCTGTGTTTCTTTTTTCTTCTATATTTTCATACATATTGTGTGAAGAGCTAAACTTTTTTTTTGGTTTGTTTCATTTGTCTTTAAGATTAGAAAGAAAAAAAACTTTCTTATATTTGGTATTTTAAGGTTAAAAGTTAAAAGATATAGTTTCTTTTGGAAAAATACATTTAATATAGAACTATAAGGTGATCTTAGGTCTATTTTCAATATATATATGAAAAGATATATAGTTAGAGTTAGTAGGTTTACTAGGACATCTAAGCAGTTTAACACATTTGAAGCACTTCATCCATCATCTCTATCTCAAAATATTATGGAAGGAAGAAGAAAGTAGAAATTACATAAGGGTTCTGCCCGAATTGCTATCAGAATTACAGCAAAACAGTATAATTATAGCAGAAAAATCCATCAATATCAGTCTCCATGCTTTTTTAATCAATATTGATGGGTCTCTGCTGTATCTGGGCGCTTAAATCAAGCCCTTGACTGTTTGAATTATCGTTTGTGCTTAAGGAACATCTTTGGGGAGATGATAAGGGTGCTAAAAAACTGTCTACCTAAAAGAAATAATGTTCGAGTGTGCTCGTATCATTCCATCATATCCTTTTAAAATAAAAACATAAGGGTCTGGTTTCTATTCATACCAAAGTTTCAAAATATCATACTTTTAGTTTGGATTATTTCAAAAAGAAATTAAGGGAAAAATGAAAAATTATTTTAAATGAAAAATGCTAAAAGTATTTTCAAACATACTGTCAAAATGAACTACATGAAAACTTTTTTGGCTTTTTTTATTCTTTTTTTATAATTTTGATGTTTTATCTCAAAAGACAAGAAAAAAAAAACAGACGAACAGAAAAATGTTGTAATAAGGATAATTTTTTTGACTAAGTTACATTTTAGAAAGGAAATGAAGTTGTATGGAGAATTTAATTGAAGTTTACAAAATAACCTTATTCCCTTTAGTTTTGCTTTCATATTTAACCTTTTGGTTTCTAAAAGTTTGGTCCACAATGGGTTAGTTTGCTTCATAGTTATAAAAATCTTACACTTTTATCACTAAAGTTTTAGATATATTCCTTTTCTTTTTTTTGTCTTATATAGATATATGGTAATTATAATTTGTAGAAATTTTAAGAATAATTTAAGTACACACCTAAAGTTTTTTTTGTTCCGTTTTCATTATTTTCCTTGTTCGTACAGCTTTGGAGCCTTCTCCTAACTAATCTCAACTAGCTACACAAAGCTAAGCTATATGTTTGGGAGTGATTTTAGCATCTTGAATTCGTTTTTTTTCAAGTGATTGATGTTTGACCTTGAATAAAATTGATTTTTAGAAAAAACTAAAATCATTTTTTATTTTGAAGGAATTAGTTGGTTTGCTATTTAATTAATTTTTTTCAAATGATAGATGAGAATCAACCTCCAAAAATGGCTATCTAATTAATTTTTTTTAAAATATTGTAGGTTACATATTTGTGGAACCAATTTTTTTTTATTTAAATTCATTGACTGAAGTTTTTAAATGAGATATATGTTTTGAATTTTTTTTTTATGCTTATTAAACACATTTTTCTCAGTAAACAAAAACTAAAATTTAACAATAAAACAAAGAATTATTTAATAGTTGTAGAGAAAATATTTACAAATTTAATAAATACTAATATACTTATCTATTAGTGATAAATATGAAATTTACTATATTTTATAAATATTTTCTACAGTTCTATCATTCCTAACAATTCTCTTTACTTTTATCGTTAAATTTTAGTTATTATTTAGTATAAAAATGTGTTTGATAAACATCAAGAGAATTTTTTTTGGTTTAGTAAGAAAAATGTGTTTCATTTTGGAATATTTGAAAATGTCCCTAAAAAAAAAGTAAAAAACATATATTTGGTTATATATATTCTCAAACAGAAAATAGTTTCAGCCGTTTTAAGAAATAATAAAATTAAAATTAATTGAACTCTTTATAGTTCATTGAAGTTTTTTTTTTAAATAGTTTTAATTTTTTTTATTCATAACAATCCCCAAATACTTTCATTAGAGTAATTTTGATAATTCTAAAATTTCTTTTCGAACTAAATATAATACGATTATATATATCAAATTCACAAAATCAATGTTATATAAATCTTTTTTTAAGATCACTACTTCAACCCTGACTGTCAAACATACACTTAAAATCATTTTAATTCTTGTATAGGATGAGAAACAAATGGGGCGCGACGGATCCTATGCAGAATACTACATGAAGGAGCATCCAGGCATTTCCTATGAAGCAACACAGCAACATATTCAGAAGAAGATTTCTGAAGCATGGAAGACTCTGAATACAGAACATCTCTTTTCTAATATCTTCCCCACCAGTTTCACTCAGGCTTCTCTCAATATTGCTAGAGCTGTTCCTTTGGCGTATAACTATGGTAGAAACCAATCTATCATGACAGTCGAGAAGCTTATGGAACAATATTGCTAATATTTCATAATGTGGGATATAAATAATGTGGATTGGATGATTCATTGTTATATATATTATTTTGTGTGTTATGATGGGAAGTAAAGCGAAAAGTTCTTTCCCTAATAGTACTTTATAATGACATGGCTGCTTTCAGTATACTTTTAGAATCAAGCATAGAGTTCTAT

Coding sequence (CDS)

ATGTATCTTCAATCCTTTCTTGCTTCTCCCTGTCCAAAAATATTCATTTATCAAACAAAAAATCCACACAAAGTTCAGAATAACAACGCATGGCTTCAATTGAATGACCCTCTTGAAAAATCTTTTGCCAGAAAAGGGTGCATTCATGCCTTGAATTCTTCTTCATCATCCAATAAGTGCATCATCTCCAATTTGAAAATTGAGGAGTTTGGGGTTGAAACTCCAGCGAGAAAAGTGGAGATTTTAAAGGACATACTAACAGAAACAAGAGATGATCTGTGTTGGGAACGTTTGGAAATAATTGATGCAGCCCAATGGCTTGGCATTGACAACCAGTTCGAAGAAGAGATTGAAGATGTTCTTAAGAGGCAATATGATTTGATAAATGCATATCGTTTTGATGGAGACATGGATCTTCACAAAGCTGCCCTCCTTTTTCGACTTTTGAGACAACAACGATACCTTATATCTCAAGGATGA

Protein sequence

MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKCIISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG

Homology

[Close]

BLAST of Cucsat.G14348 vs. ExPASy Swiss-Prot
Match: B9RXW4 (Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.2e-08
Identity = 42/109 (38.53%), Postives = 61/109 (55.96%), Query Frame = 0


Query: 56  SSNKCIISNLK-----IEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGID 115
           S N+ ++S L      + +  V    R+    K IL +  +    E L +IDA Q LGID
Sbjct: 43  SINQTLVSPLSAQCIHLTDEDVRIKERRTRAFKHILRKEGEG-SHEVLAMIDAIQRLGID 102

Query: 116 NQFEEEIEDVLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 160
           + F++EI+++L+RQY + + Y    D DLH  AL FRLLRQ  Y +S G
Sbjct: 103 HHFQDEIDEILQRQYTIPSYY---NDNDLHGLALRFRLLRQGGYNVSAG 147

[Close]

BLAST of Cucsat.G14348 vs. ExPASy Swiss-Prot
Match: P0CV94 ((3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.7e-08
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 76  ARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDG 135
           ++K+E+LK +L    +    E L +IDA Q LGID  F+ EI+++L +Q  +++A     
Sbjct: 19  SQKLELLKTVLRNVAELDALEGLNMIDAVQRLGIDYNFQREIDEILHKQMSIVSARD--- 78

Query: 136 DMDLHKAALLFRLLRQQRYLISQ 159
             DLH+ AL FRLLRQ  Y + +
Sbjct: 79  --DLHEVALRFRLLRQHGYFVPE 96

[Close]

BLAST of Cucsat.G14348 vs. ExPASy Swiss-Prot
Match: U3LVZ7 (Beta-caryophyllene synthase OS=Lavandula angustifolia OX=39329 GN=CARS PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.9e-08
Identity = 36/82 (43.90%), Postives = 51/82 (62.20%), Query Frame = 0


Query: 73  ETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYR 132
           E   R+ E ++++L +TRDD    +LE++DA Q LGI   FEEEI + L+  YD    Y 
Sbjct: 45  EEHERQKEAIRNLLLQTRDDSTL-KLELVDAIQRLGIGYHFEEEIHNSLRNIYDTNPIYN 104

Query: 133 FDGDMDLHKAALLFRLLRQQRY 155
            + D +L  AAL FRL+RQQ +
Sbjct: 105 AEDD-NLRVAALRFRLIRQQGF 124

[Close]

BLAST of Cucsat.G14348 vs. ExPASy Swiss-Prot
Match: P0CV95 ((3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.0e-07
Identity = 31/82 (37.80%), Postives = 49/82 (59.76%), Query Frame = 0


Query: 77  RKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDGD 136
           +K+E+ +++L    +    E L +IDA Q LGID  F+ EI+++L +Q   ++A      
Sbjct: 79  QKLELFRNVLRNVAELDALEGLNMIDAVQRLGIDFHFQREIDEILHKQMSNVSA-----S 138

Query: 137 MDLHKAALLFRLLRQQRYLISQ 159
            DLH+ AL FRLLRQ  Y + +
Sbjct: 139 DDLHEVALRFRLLRQHGYFVPE 155

[Close]

BLAST of Cucsat.G14348 vs. ExPASy Swiss-Prot
Match: P0CV96 ((3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca OX=57918 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 6.6e-07
Identity = 30/83 (36.14%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 76  ARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDG 135
           ++K+E+ +++L    +    E L +IDA Q LGID  F+ EI+++L +Q  +++A     
Sbjct: 80  SQKLELFRNVLRNAAELDALEGLNMIDAVQRLGIDYHFQREIDEILHKQMGIVSACD--- 139

Query: 136 DMDLHKAALLFRLLRQQRYLISQ 159
             DL++ AL FRLLRQ  Y + +
Sbjct: 140 --DLYEVALRFRLLRQHGYFVPE 157

[Close]

BLAST of Cucsat.G14348 vs. NCBI nr
Match: XP_011652895.1 (probable terpene synthase 13 isoform X2 [Cucumis sativus])

HSP 1 Score: 326 bits (836), Expect = 1.08e-112
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60
           MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120
           IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 159
           LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 159

[Close]

BLAST of Cucsat.G14348 vs. NCBI nr
Match: XP_011652894.1 (myrcene synthase, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 326 bits (836), Expect = 8.21e-112
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60
           MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120
           IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 159
           LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 159

[Close]

BLAST of Cucsat.G14348 vs. NCBI nr
Match: KAE8652686.1 (hypothetical protein Csa_013547, partial [Cucumis sativus])

HSP 1 Score: 323 bits (829), Expect = 1.22e-111
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60
           MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120
           IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQ 158
           LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQ
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQ 158

[Close]

BLAST of Cucsat.G14348 vs. NCBI nr
Match: XP_016903707.1 (PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo])

HSP 1 Score: 260 bits (664), Expect = 4.93e-81
Identity = 135/159 (84.91%), Postives = 143/159 (89.94%), Query Frame = 0


Query: 1   MYLQSFLA-SPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNK 60
           +YLQSFLA  P PKIFI QTK PHK  NNNAWLQLN+PLEKSFA KG IHALNSSSSSNK
Sbjct: 5   IYLQSFLAFPPRPKIFISQTKIPHKAHNNNAWLQLNNPLEKSFAEKGFIHALNSSSSSNK 64

Query: 61  CIISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIED 120
            IIS+LKIEE GVETPARKV+ILK++LTETRDDLC E LEIIDAAQ LGIDNQFEEEIED
Sbjct: 65  IIISDLKIEELGVETPARKVKILKELLTETRDDLCLECLEIIDAAQRLGIDNQFEEEIED 124

Query: 121 VLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQ 158
           VLKRQY LINAY FDGD+DLHKAALLFRLLRQQRYL+SQ
Sbjct: 125 VLKRQYALINAYHFDGDVDLHKAALLFRLLRQQRYLVSQ 163

[Close]

BLAST of Cucsat.G14348 vs. NCBI nr
Match: XP_038893983.1 ((3S,6E)-nerolidol synthase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 193 bits (490), Expect = 9.98e-56
Identity = 108/158 (68.35%), Postives = 122/158 (77.22%), Query Frame = 0


Query: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQL-NDPLEKSFARKGCIHALNSSSSSNK 60
           +Y Q  LAS  PKI + QTK PHKV +  A LQL N+P  K FA+K  I+AL+SSSS   
Sbjct: 5   IYHQPILASSTPKILMSQTKIPHKVHHKKALLQLKNNPPRKPFAKKWSIYALDSSSSKP- 64

Query: 61  CIISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIED 120
             ISNLKIEEFG+E P  KV+ILK+ L +TRD LCWE LEIIDAAQ LGIDN F+EEIE 
Sbjct: 65  --ISNLKIEEFGLEAPDGKVKILKEALKQTRD-LCWECLEIIDAAQRLGIDNHFKEEIET 124

Query: 121 VLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLIS 157
           +LKRQYDLINA  FDGDMDLHKAALLFRLLRQQ YL+S
Sbjct: 125 ILKRQYDLINANHFDGDMDLHKAALLFRLLRQQGYLVS 158

[Close]

BLAST of Cucsat.G14348 vs. ExPASy TrEMBL
Match: A0A0A0LX82 (Terpene_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G070080 PE=4 SV=1)

HSP 1 Score: 326 bits (836), Expect = 3.98e-112
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60
           MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120
           IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 159
           LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 159

[Close]

BLAST of Cucsat.G14348 vs. ExPASy TrEMBL
Match: A0A1S4E662 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103489227 PE=4 SV=1)

HSP 1 Score: 260 bits (664), Expect = 2.39e-81
Identity = 135/159 (84.91%), Postives = 143/159 (89.94%), Query Frame = 0


Query: 1   MYLQSFLA-SPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNK 60
           +YLQSFLA  P PKIFI QTK PHK  NNNAWLQLN+PLEKSFA KG IHALNSSSSSNK
Sbjct: 5   IYLQSFLAFPPRPKIFISQTKIPHKAHNNNAWLQLNNPLEKSFAEKGFIHALNSSSSSNK 64

Query: 61  CIISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIED 120
            IIS+LKIEE GVETPARKV+ILK++LTETRDDLC E LEIIDAAQ LGIDNQFEEEIED
Sbjct: 65  IIISDLKIEELGVETPARKVKILKELLTETRDDLCLECLEIIDAAQRLGIDNQFEEEIED 124

Query: 121 VLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQ 158
           VLKRQY LINAY FDGD+DLHKAALLFRLLRQQRYL+SQ
Sbjct: 125 VLKRQYALINAYHFDGDVDLHKAALLFRLLRQQRYLVSQ 163

[Close]

BLAST of Cucsat.G14348 vs. ExPASy TrEMBL
Match: A0A6J1EK53 ((3S,6E)-nerolidol synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434067 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.93e-20
Identity = 56/101 (55.45%), Postives = 69/101 (68.32%), Query Frame = 0


Query: 57  SNKCIISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEE 116
           +NK   S   I+  GVE    KVE LK +L E+ D  C E L++IDAAQ LGID+  +EE
Sbjct: 41  NNKTQFSTTLIDGHGVEGAVAKVESLKHVLRESGD--CLECLDLIDAAQRLGIDSLLQEE 100

Query: 117 IEDVLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLIS 157
           IE VLKRQY L+NA  FD  +DLHKAALLFR L QQ +L++
Sbjct: 101 IEAVLKRQYFLLNALEFDPMIDLHKAALLFRHLTQQGFLVT 139

[Close]

BLAST of Cucsat.G14348 vs. ExPASy TrEMBL
Match: A0A0A0LUC8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G068570 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.26e-19
Identity = 67/140 (47.86%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 26  QNNNAWLQLNDPLEKSFARKGCIHALNSS------SSSNKCIISNLKIEEFGVE--TPAR 85
           QNNN    +N+PL          H+L S+      S     I+SN      G+E    A 
Sbjct: 20  QNNNFNPIINNPLLNKQIIYLPHHSLLSTPLKPFDSKIQPSILSN------GIEYGEAAI 79

Query: 86  KVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDGDM 145
           K  ILKD+L E  D   WE L +IDA Q LGID  F+EEIE VL+RQY L NA +++ D 
Sbjct: 80  KGRILKDVLREIGDP--WECLNLIDATQRLGIDYHFQEEIEAVLQRQYVLFNAIQYNPDT 139

Query: 146 DLHKAALLFRLLRQQRYLIS 157
           DLHKAALLFRL RQQ YL+S
Sbjct: 140 DLHKAALLFRLFRQQGYLVS 151

[Close]

BLAST of Cucsat.G14348 vs. ExPASy TrEMBL
Match: A0A5D3BI68 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006300 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.05e-18
Identity = 51/82 (62.20%), Postives = 59/82 (71.95%), Query Frame = 0


Query: 76  ARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDG 135
           A K  ILKD+L E  D   WE L +IDA Q LGID  F+EEIE VL+RQY L NA +F+ 
Sbjct: 26  AIKGRILKDVLREIGDP--WECLNLIDATQRLGIDYHFQEEIEAVLQRQYVLFNAVQFNP 85

Query: 136 DMDLHKAALLFRLLRQQRYLIS 157
           + DLHKAALLFRL RQQ YL+S
Sbjct: 86  ETDLHKAALLFRLFRQQGYLVS 105

The following BLAST results are available for this feature:

BLAST of Cucsat.G14348 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9RXW4	1.2e-08	38.53	Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1	[more]
P0CV94	2.7e-08	39.76	(3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1	[more]
U3LVZ7	5.9e-08	43.90	Beta-caryophyllene synthase OS=Lavandula angustifolia OX=39329 GN=CARS PE=1 SV=1	[more]
P0CV95	5.0e-07	37.80	(3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa O...	[more]
P0CV96	6.6e-07	36.14	(3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca OX=57918 PE=1 SV=1	[more]

BLAST of Cucsat.G14348 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652895.1	1.08e-112	100.00	probable terpene synthase 13 isoform X2 [Cucumis sativus]	[more]
XP_011652894.1	8.21e-112	100.00	myrcene synthase, chloroplastic isoform X1 [Cucumis sativus]	[more]
KAE8652686.1	1.22e-111	100.00	hypothetical protein Csa_013547, partial [Cucumis sativus]	[more]
XP_016903707.1	4.93e-81	84.91	PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo]	[more]
XP_038893983.1	9.98e-56	68.35	(3S,6E)-nerolidol synthase 1-like isoform X2 [Benincasa hispida]	[more]

BLAST of Cucsat.G14348 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX82	3.98e-112	100.00	Terpene_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G0700...	[more]
A0A1S4E662	2.39e-81	84.91	(3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103489227 PE=4 S...	[more]
A0A6J1EK53	1.93e-20	55.45	(3S,6E)-nerolidol synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434067 ...	[more]
A0A0A0LUC8	1.26e-19	47.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G068570 PE=4 SV=1	[more]
A0A5D3BI68	1.05e-18	62.20	(3S,6E)-nerolidol synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 80..163 e-value: 3.8E-17 score: 62.6
IPR036965	Terpene synthase, N-terminal domain superfamily	GENE3D	1.50.10.130		coord: 55..187 e-value: 4.3E-19 score: 70.6
None	No IPR available	PANTHER	PTHR31225:SF148	(3S,6E)-NEROLIDOL SYNTHASE 2, CHLOROPLASTIC/MITOCHONDRIAL-LIKE	coord: 47..169
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 47..169
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 79..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G14348.T3	Cucsat.G14348.T3	mRNA
Cucsat.G14348.T4	Cucsat.G14348.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity