Cucsat.G14309 (gene) Cucumber (B10) v3

Overview
NameCucsat.G14309
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionL-ascorbate oxidase homolog
Locationctg1869: 5809725 .. 5814356 (-)
RNA-Seq ExpressionCucsat.G14309
SyntenyCucsat.G14309
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAGATAACCAAGTTGTTCATTTGGATTTTTAACTAAAAGGGTGCAAAATTCTTACCTTTTTTCTCCTCTTTCTTACAAGAATCTCTCCAGATCCTTCCTCTATCGCTGGAGAGATTAACGCTGTCATTGAAAATGTAGAGTTTACATTACACTAACCATATGTACCTATGCACTTGAAAGAACGACCGTCGAAGCATTTCAATATCTGAAGGTGAAGCGTCGGCGGCGGTGTTGAAGTCCCGTTGACGGTGAGTTTTACTTTGGAATGTACACTGCTTAGAGGAATCTTACATTTAATTATCCAAGCAGCACGTGAAAAGGGCAACCTTCGTAAATTCAGTCGGTCTTCATCATAGACTTTCATTTTTAACGCAATCACAACCATTCATTTCCATCTTACAAGCTGAGATTGACGGTGGATGATTTGAACAACCATCTTCCGCATTTCACTCCATCACCTACGGCTCTGAACTATAAAACTGACAACTCACAGTGCCTTCTAGGTAACATCCAAGACGGACTTCAGCTTATACATAAGGCAAAGCTCTTCATAAGTCAGAACAAAAAATTATAGAGAAAGAGAAATCAACCCTTTTGGTTCCTTTCTTTTCTCAACTCAATCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTAGATCTGTCACATTGTTCATTCCAGCTGATTTCAAGCTTCATATTGCAATGAGGGAATACAGAGTACTCTGTTCTCTCATAATCTTGGTTCTTTTTGCAGTTAATGAGGTTAATGCTGATAACCCATACAGATTTTTCACATGGAAAGTCACTTATGGCGATATCTATCCGCTTGGAGTTAAGCAACAGGTGCTTTGATTCTTTTCATTTCTATGTGCTTCCTTTTTAGAGCTAAAAGTTAGCATTTTTATCTTCTTTTCTCATCTTGAAAATGATGAGTTTGGAACTTATTGGTTGTTGTAATGAACATTTGGCAGGGAATCTTGATTAATGGGCAGTTCCCAGGACCTCAGATTGATGCTGTTACTAATGATAACTTGATTATCAATGTGTACAACTATCTCAAGGAGCCATTTCTTATTTCTTGGTAACACTACTAATTATATTTATACTTTCTATTAGCGTAATGATCTGGTTGGTTCTCGGAAATTGCATGCAAGTTTGAAACCAAATAATAGGAGTTCTCGACCTGGGATACTTACTTGGCTTGTCTGATATCAAGGCCAAACATAATTAATTTCTTGTCTGTTACCCAGCTAATGGTGTGCATTTGCTCTTACTAAAGAGTGGCAAAGGTCGAATTGTTGCATTCAGTAACATAGGGAAATGTGCCAATAATTAGAAATTGGAGGGAAAATGAACACTTGGGAAAGTTTCTAGTCATTTTCTAGATCTAAGTTATGAAGGCAGTAAAGTCTTGAGTTTTTGAAATGGCTTTTTTTTTTTTTTTTTGGGGGGGGGGACGTTAAATCTTGTAAAGCAAACATCCTATTTAATCAATTTTGCCTGTCTCTTATTTTTGTTTGGTTGTTGTTTCTTTTTAAATAGGTGTCATTAAGGTTGCCTTAAACGATCCGTAAAGTAATCAAGGGCTATCTGACCTGTTGTAGTAATGGTCTATGCAATAACTAATTTGTATGCCCATCTTGGATCTTATTGATTCTTTATATTAATTTTTTGTGTAATTAAAATATTCTTTTCAACTAGCAAGCGTCTTAGGGTAGACCTCAGTGTTCACATGCTACATCTTTACATCCCTTATTTGTTTATCATTCATAAAAGTAAAAAGTTACTCCATATTTAACCACATTAAATGGCTTAAATAAGGCAAAGTTATATCTTTGAATCTTGGCCTGCATAGTTTACAGAATTTTTTTTTTATCTGAACTGCATTAAATCTGATGGGTCCTTTGTAAGGTGAAGAAATCACTACATTTATTCCACTTTTTGTACCTTCAACTTTATGGATTTTTCAACATAAGAACAGAATAGGAAAAATAAGTTGGAAAAAGCGATTGTAATACGTTTTTAGTAAACAGAAACAAAAACAAAAATTAGTTGTGAAATTTAAACACCATCAAGTGCTCTAAAACCCTTTTAACAATTTAACCTATTGAATCTGATTGTGATTTGAGATGTTTGGGTATAAGATACAACGTTGACATTGTATTAGGATGAGATTCTAACCTTGAAGTAAATTGAGATGTAACCATTTCTTGTCGATTTAACTTTACAGGAATGGATTACAGCAGAGAAGGAACTCATGGCAAGATGGAGTTTATGGGACTACTTGTGCTATTCCTCCAATGAGGAACTTCACTTATACTCTACAGGCAAAGGACCAAATTGGCAGTTACTTCTACTTCCCATCAACAGCATTTCACAAAGCAGCTGGAGCATTTGGAGGCATTAGAATTTGGAGCCGCCCCGGAATTCCTGTTCCTTTTGCTCCTCCTGCAGGTGACTTCACAGTGCTAGCTGGTGACTGGTACAAGACCAATCATTATGTAAGCATTCTATGCTCTAATAAAAAATTTCCATGTGGATTTTGTCCCTGTTTGATTCCCTTTCTTTTTATTGTTCTTCATTCTCTGAGGCAATGGTGTTACCATTTGTCTTACGCTGTAAAATTCTGTTATAGATGGTTTCTCATTCTTTCTTAGATTATGAGACAAGTCCTGGATAGCGGTAAAAACATTCCATCTCCAGACGGGCTTCTAATCAACGGCCGAGGGTGGAATGGATATACATTCAATGTTGATCCAGGTGAACAACAGTATTTGCTGCTAACTCTGTTCATAATTCCACTGATGTGTACACAATGAAAACTTTTATTAGTTACAGAAAGTAAATTTGTTAAGCTTAATATTTGTTTTTCAGGGAAGACGTATAGGTTCAGAATTTCAAATGTGGGCATTTCAGCTTCCATTAACTTCAGAATTCAAGGCCATTTGATGAAACTGGTGGAGGTCGAAGGATCTCACACCCTCCAGAACACGTATTCCTCTCTTGACATCCATTTGGGGCAATCCTACTCTGTCTTGGTCACTGCTAATCAGCCTCCTAAGGATTACTATGTTGTTGTTTCGTCTCGATTCACTTCTCCGATCCTCACGACGACAGCTGTACTTCACTACAGCAATTCATGGCAGAAAGTTTCTGGTCCAGTTCCAGGTGGCCCGACCACTGAGATCGCGTGGTCTCTCCAGCAGGCTAGATCGATCCGGTATTTCGTCAACTCCTTATTAGCATTTCAATTGGTTTTTAGTCTAGTATTGTTTGAAATATTATTGACGATTTAAGTTACCTTCATCTGGTAATATAATTTTGGAAAACTTCAAGATCAGAAAGAAATATCTCATCTTTCATGTCGAAATGAAGAGGAAAAACTTGCAAAACATGGAAGTTGTCTATAAACATATCCGGATTACTTGAGAAAGCAACAGTTACTTTGAAGTTAATCCTGTTTGCAAGATTTTGTGTCATAGCTTGTTAAAATATGGTTTCAATCTCTTCTGCAATCAGATGGAACTTAACTGCAAGTGGACCTAGACCCAATCCACAAGGCTCCTACCATTACGGACTAATCAAGACGAGCCGTACAATAGTACTAGCAAACTCAGCTCCCGTTATTAATGGTAAGCAGAGGTTTGCTGTCAACAGCGTCTCATTTGTTCAAGCAGACACTCCACTGAAATTGGCTGACTACTTCAAGATCCCTGGAGTTTTCAATCTCAACAACATTCCCACCAATCCCACTTGGGGAAATGCCTACCTCCAGACCTCTGTCATGGGTTCTAATTTTCGAGAATACATCGAGATCGTGTTTCAGAATTGGGAAAACACTGTGCAGTCTTGGCATATCAGTGGCTACTCTTTCTTCGTTGTTGGGTAGGGCTTCTTTCCCTGTTTTTTTCAATGAACCAAAGGATCAGTGTTTCAGTCTCATTAACATGTAAGCTGGATTTGATTACAGAATGGATGGAGGCCAATGGACTCCTGCCAGCCGATCTCGATACAATCTAAGAGACACGGTTGCCCGTTGCACAACTCAGGTAATTCCCGCTTAGAAATGCTAGAGATCTGTCTATATATGTGTCTCCAAACTTGACCTTATGATAGTTTATTTTGTTATGAACTTCAGGTATATCCAAAGTCATGGACAGCAATCTATATGGCACTTGATAATGTAGGAATGTGGAATGTGAGATCTGAAAGTTGGGCAAGACAGTACTTGGGACAGCAGTTCTATCTCAGGGTTTATACCTCATCAACCTCTTTCAGAGATGAATTGCCAATCCCCCGAAATGCTCTTCTTTGCGGCAGGGCCAGTGGACGTCACACAAGGCCACTTTAAGTTTCAAAATCTTACCATTTTTGTCACGAGTGGGTGACATGATTCTAGATGATGATTGATTCAGCTGATGTTAGATTAGCATTTGTTTTGAATATAACTTCAAAAAGCTCAACTTGAGCTCTTGAATCAAATTTATCCCCGCTTACATTTTGCTCTATTTAGTACTTGCATGACTTGCCTAATGCTCAAACAAACTTTGAGTTAAACAAAAGAGATGAAAATGGATCGGAAATTTGTTAGGAAGATGCAGTGATTAAATGAAATGGAATAAACATACATAGTAAATACAGGAGAGAG

Coding sequence (CDS)

ATGAGGGAATACAGAGTACTCTGTTCTCTCATAATCTTGGTTCTTTTTGCAGTTAATGAGGTTAATGCTGATAACCCATACAGATTTTTCACATGGAAAGTCACTTATGGCGATATCTATCCGCTTGGAGTTAAGCAACAGGGAATCTTGATTAATGGGCAGTTCCCAGGACCTCAGATTGATGCTGTTACTAATGATAACTTGATTATCAATGTGTACAACTATCTCAAGGAGCCATTTCTTATTTCTTGGAATGGATTACAGCAGAGAAGGAACTCATGGCAAGATGGAGTTTATGGGACTACTTGTGCTATTCCTCCAATGAGGAACTTCACTTATACTCTACAGGCAAAGGACCAAATTGGCAGTTACTTCTACTTCCCATCAACAGCATTTCACAAAGCAGCTGGAGCATTTGGAGGCATTAGAATTTGGAGCCGCCCCGGAATTCCTGTTCCTTTTGCTCCTCCTGCAGGTGACTTCACAGTGCTAGCTGGTGACTGGTACAAGACCAATCATTATATTATGAGACAAGTCCTGGATAGCGGTAAAAACATTCCATCTCCAGACGGGCTTCTAATCAACGGCCGAGGGTGGAATGGATATACATTCAATGTTGATCCAGGGAAGACGTATAGGTTCAGAATTTCAAATGTGGGCATTTCAGCTTCCATTAACTTCAGAATTCAAGGCCATTTGATGAAACTGGTGGAGGTCGAAGGATCTCACACCCTCCAGAACACGTATTCCTCTCTTGACATCCATTTGGGGCAATCCTACTCTGTCTTGGTCACTGCTAATCAGCCTCCTAAGGATTACTATGTTGTTGTTTCGTCTCGATTCACTTCTCCGATCCTCACGACGACAGCTGTACTTCACTACAGCAATTCATGGCAGAAAGTTTCTGGTCCAGTTCCAGGTGGCCCGACCACTGAGATCGCGTGGTCTCTCCAGCAGGCTAGATCGATCCGATGGAACTTAACTGCAAGTGGACCTAGACCCAATCCACAAGGCTCCTACCATTACGGACTAATCAAGACGAGCCGTACAATAGTACTAGCAAACTCAGCTCCCGTTATTAATGGTAAGCAGAGGTTTGCTGTCAACAGCGTCTCATTTGTTCAAGCAGACACTCCACTGAAATTGGCTGACTACTTCAAGATCCCTGGAGTTTTCAATCTCAACAACATTCCCACCAATCCCACTTGGGGAAATGCCTACCTCCAGACCTCTGTCATGGGTTCTAATTTTCGAGAATACATCGAGATCGTGTTTCAGAATTGGGAAAACACTGTGCAGTCTTGGCATATCAGTGGCTACTCTTTCTTCGTTGTTGGAATGGATGGAGGCCAATGGACTCCTGCCAGCCGATCTCGATACAATCTAAGAGACACGGTTGCCCGTTGCACAACTCAGGTATATCCAAAGTCATGGACAGCAATCTATATGGCACTTGATAATGTAGGAATGTGGAATGTGAGATCTGAAAGTTGGGCAAGACAGTACTTGGGACAGCAGTTCTATCTCAGGGTTTATACCTCATCAACCTCTTTCAGAGATGAATTGCCAATCCCCCGAAATGCTCTTCTTTGCGGCAGGGCCAGTGGACGTCACACAAGGCCACTTTAA

Protein sequence

MREYRVLCSLIILVLFAVNEVNADNPYRFFTWKVTYGDIYPLGVKQQGILINGQFPGPQIDAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPMRNFTYTLQAKDQIGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWYKTNHYIMRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMKLVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLANSAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGSNFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRASGRHTRPL
Homology
BLAST of Cucsat.G14309 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 5.8e-107
Identity = 192/366 (52.46%), Postives = 241/366 (65.85%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGR-----GWNGYTFNVDPGKTYRFRISNVGISASINFRIQ 60
           +++ LD G+ I  PDG++ING+     G +   F + PGKTYR RI NVG+  SINFRIQ
Sbjct: 176 LKKFLDGGRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQ 235

Query: 61  GHLMKLVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTA 120
            H MKLVE+EGSH LQN Y SLD+H+GQ +  +VTANQ PKDYY+V SSRF   ++TTT 
Sbjct: 236 NHKMKLVEMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTG 295

Query: 121 VLHYSNSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRT 180
           +L Y       S  +P GP    AWSL Q RS RWNLTAS  RPNPQGSYHYG I  +RT
Sbjct: 296 LLRYEGGKGPASSQLPAGP-VGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRT 355

Query: 181 IVLANSAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPG-VFNLNNIPTNPT---WGNA 240
           I L N+   ++GK RFA+N VS  + +TPLKLA+YF I   VF  + I  +PT     N 
Sbjct: 356 IKLVNTQGKVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNI 415

Query: 241 YLQTSVMGSNFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTV 300
            ++ +V+    R ++E+VF+N E +VQSWH+ GYSFF V ++ G WTP  R  YNL D V
Sbjct: 416 KIEPNVLNITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAV 475

Query: 301 ARCTTQVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPR 358
           +R T QVYPK W AI +  DN GMWNVRSE+  R+YLGQQ Y  V +   S RDE  +P 
Sbjct: 476 SRHTVQVYPKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPE 535

BLAST of Cucsat.G14309 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.5e-99
Identity = 178/377 (47.21%), Postives = 241/377 (63.93%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRG-----WNGYTFNVDPGKTYRFRISNVGISASINFRIQ 60
           ++++LD G+ I  PDG++ING+           F ++ GKTYR+R  N+G+ +S+N R Q
Sbjct: 174 LKKILDGGRTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQ 233

Query: 61  GHLMKLVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTA 120
           GH MKLVE+EGSHT+QN Y SLD+H+GQ  SVLVTA+Q PKDYY+VVSSRF    L++ A
Sbjct: 234 GHPMKLVELEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVA 293

Query: 121 VLHYSNSWQKVSGPVPGGP---TTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKT 180
           ++ Y+N     S  +P  P   T  IAWS+ Q RS RWNLTAS  RPNPQGSYHYG I  
Sbjct: 294 IIRYANGKGPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINI 353

Query: 181 SRTIVLANSAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFN--------LNNIPT 240
           +RTI + NS   + GK R+ +N +S    +TPLKL +YF   G  N         +  P 
Sbjct: 354 TRTIKIFNSMSQVGGKLRYGLNGISHTNGETPLKLVEYF---GATNKAFKYDLMADEAPA 413

Query: 241 NPTWGNAYLQTSVMGSNFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSR 300
           +P+     + T+V  + +R ++EI+F+N E T++++H+ GYSFF V ++ G+W+P  R  
Sbjct: 414 DPS--KLTIATNVKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKN 473

Query: 301 YNLRDTVARCTTQVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFR 360
           YNL D ++R   QVYP SW AI +  DN GMWN+RSE W + YLG+Q Y  V + S S R
Sbjct: 474 YNLVDGLSRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLR 533

Query: 361 DELPIPRNALLCGRASG 362
           DE  IP N  LCG   G
Sbjct: 534 DEYNIPDNHPLCGIVKG 545

BLAST of Cucsat.G14309 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 8.7e-95
Identity = 182/380 (47.89%), Postives = 243/380 (63.95%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGY------------TFNVDPGKTYRFRISNVGISA 60
           +R+ LD GK++  PDG+LING+G   Y            T  V PGKTYR R+SNVGIS 
Sbjct: 173 LRKALDDGKDLGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGIST 232

Query: 61  SINFRIQGHLMKLVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQ-PPKDYYVVVSSRFT 120
           S+NFRIQGH + L E EGS+T+Q  Y+SLDIH+GQSYS LVT +Q    DYY+V S+R  
Sbjct: 233 SLNFRIQGHNLVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVV 292

Query: 121 SPIL----TTTAVLHYSNSWQKVSGPVPGGPTTEI--AWSLQQARSIRWNLTASGPRPNP 180
           +  +    T   +L Y+NS  K  G +P GP  E    +S+ QARSIRWN++ASG RPNP
Sbjct: 293 NETIWRRVTGVGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNP 352

Query: 181 QGSYHYGLIKTSRTIVLANSAPV-INGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLN 240
           QGS+ YG I  +   VL N  PV I+GK+R  +N +SF    TP++LAD  K+  V+ L 
Sbjct: 353 QGSFKYGSINVTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKL- 412

Query: 241 NIPTNPTWGNAYLQTSVMGSNFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPA 300
           + P  P  G A + TS++   +R ++E+V QN +  +QS+H+SGY+FFVVGMD G+WT  
Sbjct: 413 DFPKRPLTGPAKVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTEN 472

Query: 301 SRSRYNLRDTVARCTTQVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSS 360
           SR  YN  D +AR T QVYP +W+AI ++LDN G WN+R+E+    YLGQ+ Y+RV    
Sbjct: 473 SRGTYNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPD 532

BLAST of Cucsat.G14309 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.7e-90
Identity = 172/377 (45.62%), Postives = 242/377 (64.19%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGY-----------TFNVDPGKTYRFRISNVGISAS 60
           +R+ LDSGK +  PDG+LING+G   Y           TF+V+PGKTYR R+ NVGIS S
Sbjct: 177 LRRALDSGKELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTS 236

Query: 61  INFRIQGHLMKLVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQ-PPKDYYVVVSSRFTS 120
           +NFRIQ H + LVE EG +T Q  ++  D+H+GQSYS LVT +Q    DYY+V S+RF +
Sbjct: 237 LNFRIQNHSLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVN 296

Query: 121 PI----LTTTAVLHYSNSWQKVSGPVPGGPTTEIA--WS-LQQARSIRWNLTASGPRPNP 180
                 +T  A+LHYSNS   VSGP+P  P T+++  WS + Q ++IR N +ASG RPNP
Sbjct: 297 ETVWQRVTGVAILHYSNSKGPVSGPLP-VPKTDVSSPWSAMSQPKTIRQNTSASGARPNP 356

Query: 181 QGSYHYGLIKTSRTIVLANSAP-VINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLN 240
           QGS+HYG I  + T +L +  P +ING  R  +N +SFV   TP++LAD  K+ G + L 
Sbjct: 357 QGSFHYGQINITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKL- 416

Query: 241 NIPTNPTWGNAYLQTSVMGSNFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPA 300
           + P  P      L  S++ + ++ +I++VFQN +  +QS+H+ GYSFFVVGMD G W+  
Sbjct: 417 DFPDRPFNRPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSED 476

Query: 301 SRSRYNLRDTVARCTTQVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSS 358
            +  YN  D ++R T +VYP  WTA+ ++LDNVG+WN+R E+  R YLG++ Y+R+    
Sbjct: 477 KKGSYNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPE 536

BLAST of Cucsat.G14309 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 4.2e-89
Identity = 171/377 (45.36%), Postives = 238/377 (63.13%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGY-----------TFNVDPGKTYRFRISNVGISAS 60
           +R++LDSGK +  PDG+LING+G   Y           T NVDPGKTYR R+ NVGIS S
Sbjct: 176 LRRILDSGKELGMPDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTS 235

Query: 61  INFRIQGHLMKLVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQ-PPKDYYVVVSSRFTS 120
           +NFRIQ H + L+E EG +T Q  ++  D+H+GQSYS LVT +Q    DYY+V S+RF +
Sbjct: 236 LNFRIQNHKLLLIETEGRYTSQMNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVN 295

Query: 121 PI----LTTTAVLHYSNSWQKVSGPVPGGPT-TEIAWS-LQQARSIRWNLTASGPRPNPQ 180
                 +T   +LHYSNS    SGP+P   T     WS + Q R+I+ N +ASG RPNPQ
Sbjct: 296 ETVWQRVTGVGILHYSNSKGPASGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQ 355

Query: 181 GSYHYGLIKTSRTIVLANSAPV-INGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNN 240
           GS+HYG I  +RT +L +  P  INGK R  +N +SFV   TP++LAD  K+ G + L +
Sbjct: 356 GSFHYGQINITRTYILRSLPPTKINGKLRATLNGISFVNPSTPMRLADDHKVKGDYML-D 415

Query: 241 IPTNPTWGN-AYLQTSVMGSNFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPA 300
            P  P       L +S++ + ++ +I+++FQN +  +QS+HI GY+F+VV MD G W+  
Sbjct: 416 FPDRPLDEKLPRLSSSIINATYKGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSED 475

Query: 301 SRSRYNLRDTVARCTTQVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSS 358
             S YN  D VAR T +VYP +WTA+ ++LDNVG+WN+R E+  R YLGQ+ Y+R+    
Sbjct: 476 RNSSYNNWDAVARSTVEVYPGAWTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPE 535

BLAST of Cucsat.G14309 vs. NCBI nr
Match: XP_004153223.1 (L-ascorbate oxidase homolog [Cucumis sativus] >KAE8652663.1 hypothetical protein Csa_013262 [Cucumis sativus])

HSP 1 Score: 760 bits (1962), Expect = 1.50e-274
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK
Sbjct: 176 MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS
Sbjct: 356 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP
Sbjct: 416 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS
Sbjct: 476 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. NCBI nr
Match: XP_008440923.1 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo] >ADN34061.1 multicopper oxidase [Cucumis melo subsp. melo])

HSP 1 Score: 750 bits (1937), Expect = 9.64e-271
Identity = 360/367 (98.09%), Postives = 365/367 (99.46%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +R+VLDSGKN+PSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK
Sbjct: 176 LRRVLDSGKNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAPVINGKQRFAVN VSFVQADTPLKLADYFKIPGVFNLN+IPTNPTWGNAYLQTSVMGS
Sbjct: 356 SAPVINGKQRFAVNGVSFVQADTPLKLADYFKIPGVFNLNSIPTNPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASR RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRGRYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           K+WTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS
Sbjct: 476 KAWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. NCBI nr
Match: XP_038893761.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 743 bits (1918), Expect = 7.53e-268
Identity = 354/367 (96.46%), Postives = 366/367 (99.73%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +RQVL+SGKN+PSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGH+MK
Sbjct: 176 LRQVLESGKNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHVMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTI+LAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLN+IPT PTWGNAYLQTSVMGS
Sbjct: 356 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREYIEIVFQNWE+TVQSWHI+GYSFFVVGMDGGQWTPASR+RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYIEIVFQNWEDTVQSWHINGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           K+WTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNAL+CG+AS
Sbjct: 476 KAWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALVCGKAS 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. NCBI nr
Match: XP_023001247.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 735 bits (1897), Expect = 1.19e-264
Identity = 348/367 (94.82%), Postives = 364/367 (99.18%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +R+VL+SG+N+PSPDGLLINGRGWNGYTFNV+PGKTYRFRISNVG+SASINFRIQGH+MK
Sbjct: 176 LRRVLESGRNLPSPDGLLINGRGWNGYTFNVNPGKTYRFRISNVGLSASINFRIQGHVMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTA+QPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTI+LAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAP+INGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLN+IPT PTWGNAYLQTSVMGS
Sbjct: 356 SAPIINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREY+EIVFQNWE+TVQSWHI GYSFFVVGMDGGQWTPASR+RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYVEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           K+WTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRA 
Sbjct: 476 KAWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAR 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. NCBI nr
Match: XP_022923951.1 (L-ascorbate oxidase homolog [Cucurbita moschata] >KAG6584095.1 L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 734 bits (1895), Expect = 2.39e-264
Identity = 348/367 (94.82%), Postives = 364/367 (99.18%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +R+VL+SG+N+PSPDGLLINGRGWNGYTFNV+PGKTYRFRISNVG+SASINFRIQGH+MK
Sbjct: 176 LRRVLESGRNLPSPDGLLINGRGWNGYTFNVNPGKTYRFRISNVGLSASINFRIQGHVMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTA+QPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTI+LAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLN+IPT PTWGNAYLQTSVMGS
Sbjct: 356 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREY+EIVFQNWE+TVQSWHI GYSFFVVGMDGGQWTPASR+RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYVEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           ++WTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRA 
Sbjct: 476 RAWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAR 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. ExPASy TrEMBL
Match: E5GC62 (Multicopper oxidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 750 bits (1937), Expect = 4.67e-271
Identity = 360/367 (98.09%), Postives = 365/367 (99.46%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +R+VLDSGKN+PSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK
Sbjct: 176 LRRVLDSGKNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAPVINGKQRFAVN VSFVQADTPLKLADYFKIPGVFNLN+IPTNPTWGNAYLQTSVMGS
Sbjct: 356 SAPVINGKQRFAVNGVSFVQADTPLKLADYFKIPGVFNLNSIPTNPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASR RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRGRYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           K+WTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS
Sbjct: 476 KAWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. ExPASy TrEMBL
Match: A0A1S3B2W1 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103485195 PE=3 SV=1)

HSP 1 Score: 750 bits (1937), Expect = 4.67e-271
Identity = 360/367 (98.09%), Postives = 365/367 (99.46%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +R+VLDSGKN+PSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK
Sbjct: 176 LRRVLDSGKNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAPVINGKQRFAVN VSFVQADTPLKLADYFKIPGVFNLN+IPTNPTWGNAYLQTSVMGS
Sbjct: 356 SAPVINGKQRFAVNGVSFVQADTPLKLADYFKIPGVFNLNSIPTNPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASR RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRGRYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           K+WTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS
Sbjct: 476 KAWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. ExPASy TrEMBL
Match: A0A6J1KG06 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111495434 PE=3 SV=1)

HSP 1 Score: 735 bits (1897), Expect = 5.74e-265
Identity = 348/367 (94.82%), Postives = 364/367 (99.18%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +R+VL+SG+N+PSPDGLLINGRGWNGYTFNV+PGKTYRFRISNVG+SASINFRIQGH+MK
Sbjct: 176 LRRVLESGRNLPSPDGLLINGRGWNGYTFNVNPGKTYRFRISNVGLSASINFRIQGHVMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTA+QPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTI+LAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAP+INGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLN+IPT PTWGNAYLQTSVMGS
Sbjct: 356 SAPIINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREY+EIVFQNWE+TVQSWHI GYSFFVVGMDGGQWTPASR+RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYVEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           K+WTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRA 
Sbjct: 476 KAWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAR 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. ExPASy TrEMBL
Match: A0A6J1E844 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111431515 PE=3 SV=1)

HSP 1 Score: 734 bits (1895), Expect = 1.16e-264
Identity = 348/367 (94.82%), Postives = 364/367 (99.18%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +R+VL+SG+N+PSPDGLLINGRGWNGYTFNV+PGKTYRFRISNVG+SASINFRIQGH+MK
Sbjct: 176 LRRVLESGRNLPSPDGLLINGRGWNGYTFNVNPGKTYRFRISNVGLSASINFRIQGHVMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTA+QPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTI+LAN
Sbjct: 296 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLN+IPT PTWGNAYLQTSVMGS
Sbjct: 356 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREY+EIVFQNWE+TVQSWHI GYSFFVVGMDGGQWTPASR+RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYVEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           ++WTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRA 
Sbjct: 476 RAWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAR 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

BLAST of Cucsat.G14309 vs. ExPASy TrEMBL
Match: A0A6J1CGS5 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111010709 PE=3 SV=1)

HSP 1 Score: 733 bits (1892), Expect = 3.31e-264
Identity = 348/367 (94.82%), Postives = 362/367 (98.64%), Query Frame = 0

Query: 1   MRQVLDSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMK 60
           +RQVLDSG+N+PSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVG+SASINFRIQGHLMK
Sbjct: 176 LRQVLDSGRNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGLSASINFRIQGHLMK 235

Query: 61  LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYS 120
           LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTA+QPPKDYYVVVSSRFTSPILTTTAVLHYS
Sbjct: 236 LVEVEGSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYS 295

Query: 121 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLAN 180
           NSWQK+SGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTI+LAN
Sbjct: 296 NSWQKISGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILAN 355

Query: 181 SAPVINGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGS 240
           SAP+INGKQRFAVNSVSFVQADTPLKLAD+FKI GVFNLN+IPT PTWGNAYLQTSVMGS
Sbjct: 356 SAPIINGKQRFAVNSVSFVQADTPLKLADFFKISGVFNLNSIPTTPTWGNAYLQTSVMGS 415

Query: 241 NFREYIEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYP 300
           NFREYIEIV QNWE+TVQSWHI+GYSFFVVGMDGGQWTPASR+RYNLRDTVARCTTQVYP
Sbjct: 416 NFREYIEIVLQNWEDTVQSWHINGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP 475

Query: 301 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRAS 360
           KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTS RDELP+PRN LLCGRAS
Sbjct: 476 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPVPRNXLLCGRAS 535

Query: 361 GRHTRPL 367
           GRHTRPL
Sbjct: 536 GRHTRPL 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006245.8e-10752.46L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291621.5e-9947.21L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU408.7e-9547.89Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX51.7e-9045.62Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN64.2e-8945.36Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
XP_004153223.11.50e-274100.00L-ascorbate oxidase homolog [Cucumis sativus] >KAE8652663.1 hypothetical protein... [more]
XP_008440923.19.64e-27198.09PREDICTED: L-ascorbate oxidase homolog [Cucumis melo] >ADN34061.1 multicopper ox... [more]
XP_038893761.17.53e-26896.46L-ascorbate oxidase homolog [Benincasa hispida][more]
XP_023001247.11.19e-26494.82L-ascorbate oxidase homolog [Cucurbita maxima][more]
XP_022923951.12.39e-26494.82L-ascorbate oxidase homolog [Cucurbita moschata] >KAG6584095.1 L-ascorbate oxida... [more]
Match NameE-valueIdentityDescription
E5GC624.67e-27198.09Multicopper oxidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3B2W14.67e-27198.09L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103485195 PE=3 SV=1[more]
A0A6J1KG065.74e-26594.82L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111495434 PE=3 SV=... [more]
A0A6J1E8441.16e-26494.82L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111431515 PE=3 S... [more]
A0A6J1CGS53.31e-26494.82L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111010709 PE=3 ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 53..189
e-value: 9.8E-41
score: 139.5
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 272..407
e-value: 1.0E-25
score: 90.0
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 3..40
e-value: 3.9E-6
score: 26.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 52..225
e-value: 6.3E-39
score: 136.1
IPR008972CupredoxinGENE3D2.60.40.420coord: 236..431
e-value: 8.1E-35
score: 122.4
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 3..67
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 48..227
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 237..426
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 2..428
NoneNo IPR availablePANTHERPTHR11709:SF11L-ASCORBATE OXIDASE HOMOLOGcoord: 2..428
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 52..187
e-value: 4.57017E-80
score: 241.923

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G14309.T1Cucsat.G14309.T1mRNA
Cucsat.G14309.T3Cucsat.G14309.T3mRNA
Cucsat.G14309.T5Cucsat.G14309.T5mRNA
Cucsat.G14309.T2Cucsat.G14309.T2mRNA
Cucsat.G14309.T8Cucsat.G14309.T8mRNA
Cucsat.G14309.T7Cucsat.G14309.T7mRNA
Cucsat.G14309.T6Cucsat.G14309.T6mRNA
Cucsat.G14309.T4Cucsat.G14309.T4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity