Cucsat.G14243 (gene) Cucumber (B10) v3

BLAST of Cucsat.G14243 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.3e-149
Identity = 269/508 (52.95%), Postives = 353/508 (69.49%), Query Frame = 0

Query: 3   PISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQKYG 62
           PI  L  FI   L        K +    PPSP RLP IGNLHQLGS PH+SL  LS+KYG
Sbjct: 29  PILCLSIFILFSLTRSSSPSEKNRKLKLPPSPPRLPWIGNLHQLGSFPHRSLRALSKKYG 88

Query: 63  PLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYW 122
            +M +  G+ P L++SSA+MA+ VMKT D+VF +RPQTTA   L Y   D+ FAPYGEYW
Sbjct: 89  DVMFMHFGKVPTLIVSSAEMAKDVMKTQDIVFCSRPQTTAPSILFYDGHDIAFAPYGEYW 148

Query: 123 RQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRK---SGSDGSLDLGHLFFRTSNNI 182
           RQ R+IC LEL S+KRV  FQY R EE+  LV+KIRK   S +   ++LG L   TSNNI
Sbjct: 149 RQVRRICVLELLSLKRVHQFQYARVEEVAELVSKIRKASASANGAPINLGELLVSTSNNI 208

Query: 183 VSRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCF 242
           + RC++G+KFE+++  + F E ++++M  + +F F DFFPS  W ID  RG+   LK+ +
Sbjct: 209 ICRCILGQKFEDKE-DNWFGETTKELMTQVMSFSFGDFFPSLKW-IDRARGYLAYLKSIW 268

Query: 243 KTLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKA 302
              D+FF K+++EHK   K        +    KKD VDI+L +Q D  LD+  +  N+KA
Sbjct: 269 LEFDKFFDKLIDEHKAAQK--------EGKPRKKDIVDILLDVQNDGSLDFELTTSNVKA 328

Query: 303 IVLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQ 362
           I+ DMFVGGSDT+ T   W M+EL + PR+MKKVQEEVR + GK+  +E  DI +M+Y+ 
Sbjct: 329 ILQDMFVGGSDTSWTAAIWLMSELSQNPRVMKKVQEEVRRVAGKRGYVEESDINEMKYLT 388

Query: 363 CVIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIP 422
           CVIKE+LRLHPP PLL+PRE M+DV++ G+ IP+KT+VFVNA+A+QRDP+ WD P+EF+P
Sbjct: 389 CVIKENLRLHPPAPLLLPREAMSDVKLGGFDIPAKTQVFVNAYAVQRDPKVWDKPDEFMP 448

Query: 423 ERFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDG--- 482
           ERF  + N+  + GQ+FE IPFG+GRR CPGL+FG+AS ++ LAN+LYWFDWKLP G   
Sbjct: 449 ERF--EENNVGFVGQDFELIPFGAGRRVCPGLAFGVASAQYVLANMLYWFDWKLPSGGSK 508

Query: 483 -CESLDVEEANGLTVRKKKPLHLNPIPY 504
             E+LD+ E  GLTV KK PL+L P PY
Sbjct: 509 LAETLDMSEVYGLTVHKKSPLYLVPTPY 524

BLAST of Cucsat.G14243 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 512.7 bits (1319), Expect = 4.8e-144
Identity = 248/499 (49.70%), Postives = 356/499 (71.34%), Query Frame = 0

Query: 4   ISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQKYGP 63
           +S L   I +    LKL   + K PN PPSP  LP+IGNLHQLG+LPH+SL +L+ + GP
Sbjct: 5   VSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGP 64

Query: 64  LMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYWR 123
           L+LL LG  P L++S+A++AE+++KTHDL+F++RP TTAA+ + Y C D+ F+PYGEYWR
Sbjct: 65  LILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWR 124

Query: 124 QARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDG-SLDLGHLFFRTSNNIVSR 183
           Q RKIC LEL S+KRV S++ +R+EE+  ++ +I +S S G +++L  L    S+  ++R
Sbjct: 125 QVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITR 184

Query: 184 CVMGEKFE-NEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCFKT 243
              G+K+E  E+ K++F +++ ++  ++ AF   D+FPSF W +DV+ G +  LK     
Sbjct: 185 VAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAW-VDVLTGMDARLKRNHGE 244

Query: 244 LDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIV 303
           LD F   V+++H    K +G      D   +KD VD++L LQ+D  L  H + +NLKA++
Sbjct: 245 LDAFVDHVIDDHLLSRKANG-----SDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVI 304

Query: 304 LDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCV 363
           LDMF GG+DTTA  LEW M EL++ P +M+K Q+EVR ++GKK+K+E ED+ ++ Y++ +
Sbjct: 305 LDMFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLI 364

Query: 364 IKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPER 423
           IKE+LRLHP  PLLVPRE+  DV I GY+IP+KTRVF+NAWAI RDP+ W+N  EF+PER
Sbjct: 365 IKETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPER 424

Query: 424 FMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGC--ES 483
           F++  NS D+KGQ+F+ IPFG+GRR CPG++FGI+S E +LAN+LYWF+W+LP     E 
Sbjct: 425 FVN--NSVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKED 484

Query: 484 LDVEEANGLTVRKKKPLHL 499
           LD+ EA G+TV  K PL L
Sbjct: 485 LDMSEAVGITVHMKFPLQL 495

BLAST of Cucsat.G14243 vs. ExPASy Swiss-Prot
Match: A0A0N9HTU1 (Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 9.7e-129
Identity = 226/498 (45.38%), Postives = 327/498 (65.66%), Query Frame = 0

Query: 7   LLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQKYGPLML 66
           LLF   +F+L  K   F  K PN PPSP +LP++GN HQLG+L H+++  L+ KYGPLML
Sbjct: 11  LLFISTVFILKRK---FSHK-PNLPPSPPKLPILGNFHQLGTLVHRAVTVLAAKYGPLML 70

Query: 67  LKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYWRQAR 126
           L  G+ PVL++SS + A+++MKTHDL  +NRP TTAA+ LLY C D+ FAPYGEYWR+ +
Sbjct: 71  LHFGKTPVLIVSSQETAKEIMKTHDLALANRPLTTAARALLYDCTDISFAPYGEYWREMK 130

Query: 127 KICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGS-LDLGHLFFRTSNNIVSRCVM 186
           K+  L L S+K+++SF+ VR+E    ++ +I +    G+ +D+ ++ +  S +++ RC +
Sbjct: 131 KMAVLNLLSIKKIQSFRSVREELASDMIKEITRLSKTGAPVDVTNMLYHFSEDLLFRCTL 190

Query: 187 GEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCFKTLDEF 246
           G K     G+ +F+++SR  + ++ AFCF DFFP   W +D + G N  LK   + LD+F
Sbjct: 191 GFK---PKGQHKFQKLSRDFLDLVGAFCFNDFFPGMAW-MDALTGLNRKLKKGSRELDDF 250

Query: 247 FSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIVLDMF 306
             +++EE    +K         D     +F+D++L   +D   +   + DN+KAI+LD F
Sbjct: 251 VDELIEERIAMVK---------DGVEPNEFLDLLLHTHRDTTQEIKLTRDNVKAIILDTF 310

Query: 307 VGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCVIKES 366
           +GG D  A+ +EW M ELMR P  MK  QEEVR ++G K+K++ +D+ +M +++  +KE+
Sbjct: 311 LGGIDLPASVMEWAMAELMRNPSKMKIAQEEVRKVVGNKNKVDEDDVYQMNFLKSAVKET 370

Query: 367 LRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPERFMDK 426
           LRLHPP PLL  RE+   + +E Y IP  T V +N W IQRDP+ WD   EFIPERFM+ 
Sbjct: 371 LRLHPPAPLLFARESYTSINVENYIIPPYTSVMINIWHIQRDPKLWDKAEEFIPERFMN- 430

Query: 427 TNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGC--ESLDVE 486
            +  DYK  ++EFIPFGSGRR CPG+SFG+A+ EFA+AN+LYWFDWK       E+LD+ 
Sbjct: 431 -SGIDYKSHDYEFIPFGSGRRGCPGMSFGVAAVEFAVANLLYWFDWKFVGDTTPETLDMT 489

Query: 487 EANGLTVRKKKPLHLNPI 502
           E     + KKKPLH  PI
Sbjct: 491 EDYCFALFKKKPLHFIPI 489

BLAST of Cucsat.G14243 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.3e-122
Identity = 236/511 (46.18%), Postives = 323/511 (63.21%), Query Frame = 0

Query: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNF--PPSPLRLPLIGNLHQLGSLPHQSLATLS 60
           + P++  L  IF+ LL       K  T     PPSP +LP+IGNLHQ+GS PH+SL  LS
Sbjct: 18  LQPLAFTLLAIFLVLLYTWYSSTKTTTQKSTQPPSPPKLPIIGNLHQIGSYPHRSLQALS 77

Query: 61  QKYGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPY 120
           Q++GPLMLL  G  PVLV+SSA+ A +++KTHDL FS+RP++T  + LLY  +D+  APY
Sbjct: 78  QRHGPLMLLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTIFEKLLYNYKDVASAPY 137

Query: 121 GEYWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNN 180
           GEYWRQ R IC L L S +RV SF+ VR+EE  S++  I K  S   L+L  +F R +N+
Sbjct: 138 GEYWRQVRSICVLNLLSNRRVRSFRSVREEETKSMIRNI-KGSSSSVLNLSEMFVRLTND 197

Query: 181 IVSRCVMGEKF-ENEDGKS--RFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDL 240
           +V +  +G K+ + E G+S   F+EI  +   ++      D+ P   W+  V  G    L
Sbjct: 198 VVCKVALGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYVPWLSWLSHV-NGLGAKL 257

Query: 241 KNCFKTLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLD 300
               K LD+F   VV+EH     RSG        + +KDF+DI+L +Q++          
Sbjct: 258 DKVAKQLDDFIDTVVQEHMNHSSRSGD-------DDQKDFLDILLAIQKETSAGIPIDGV 317

Query: 301 NLKAIVLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAE-DIQK 360
           ++K I+LDMF  G+DTT + LEW MTEL+R PR+M K+Q EVR I+G ++ +  E D+ +
Sbjct: 318 SVKGIILDMFAAGTDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVGNRTDVITEDDLVE 377

Query: 361 MEYMQCVIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNP 420
           M Y++ V KE+LRLHPP+PLLVPR +  DVE+ GY I + T+VF++AW I RDP+ +D P
Sbjct: 378 MHYLKAVTKETLRLHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQIGRDPKLYDKP 437

Query: 421 NEFIPERFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLP 480
            EF PERF++  N  DYKG +FE IPFG+GRR CPG+ F +A  E ALANI++ FDW LP
Sbjct: 438 EEFEPERFLN--NGIDYKGNDFELIPFGAGRRVCPGIQFAMAVNEIALANIVHKFDWALP 497

Query: 481 DGC--ESLDVEEANGLTVRKKKPLHLNPIPY 504
           D    E LD+ E  GLT  KK PL     P+
Sbjct: 498 DEASGEDLDMTETTGLTAHKKYPLKAVAFPH 517

BLAST of Cucsat.G14243 vs. ExPASy Swiss-Prot
Match: O65788 (Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 437.2 bits (1123), Expect = 2.6e-121
Identity = 229/510 (44.90%), Postives = 333/510 (65.29%), Query Frame = 0

Query: 7   LLFFIFIFLLSL--KLFFFKPKTP--NFPPSPLRLPLIGNLHQLGSLPHQSLATLSQKYG 66
           LL F  + LL++   +F  + KT   N PPSP  LP+IGNLH L  LPH+    LS KYG
Sbjct: 4   LLCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYG 63

Query: 67  PLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYW 126
           PL+ L+LG  PV+VISS++ AE V+KT+DL   +RP+T  +  L YG +D+ FAPYGEYW
Sbjct: 64  PLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYW 123

Query: 127 RQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSG-SDGSLDLGHLFFRTSNNIVS 186
           R+ RK+  +ELFS K+V+SF+Y+R+EE+D +V K+ +S      +DL   FF  + +I+ 
Sbjct: 124 REVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIIC 183

Query: 187 RCVMGEKFENEDG----KSRFEEISRKVMVIITAFCFEDFFP-SFGWIIDVIRGFNWDLK 246
           R  +G+ F NE G    + R EE+  +    +  F F DFFP   G  +D +   +  + 
Sbjct: 184 RVALGQNF-NESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKIN 243

Query: 247 NCFKTLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQL--QQDDKLDYHFSL 306
             FK LD F+  V+++H   +K  G           +D V ++L +  +Q+D   +  ++
Sbjct: 244 KVFKELDAFYQHVIDDH---LKPEG--------RKNQDIVTLILDMIDKQEDSDSFKLNM 303

Query: 307 DNLKAIVLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIG-KKSKIEAEDIQ 366
           DNLKAIV+D+F+ G DT+A  + W MTEL+R PR+MKK QE +RT +G KK +I  ED+ 
Sbjct: 304 DNLKAIVMDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLG 363

Query: 367 KMEYMQCVIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDN 426
           K+EY+  ++KE+ RLHP +P +VPRETM+ ++I+GY IP KT++ +N W I RDP+ W++
Sbjct: 364 KVEYLNHILKETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWND 423

Query: 427 PNEFIPERFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKL 486
           P EF PERF +  +S D++GQ+F+ +PFGSGRR CPG+   IAS E AL N+LY+FDW +
Sbjct: 424 PEEFNPERFAN--SSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSM 483

Query: 487 PDGC--ESLDVEEANGLTVRKKKPLHLNPI 502
           PDG   E +D+EEA  +++ KK PL L P+
Sbjct: 484 PDGTKGEDIDMEEAGNISIVKKIPLQLVPV 499

BLAST of Cucsat.G14243 vs. NCBI nr
Match: XP_031739495.1 (cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 1019 bits (2634), Expect = 0.0
Identity = 504/507 (99.41%), Postives = 506/507 (99.80%), Query Frame = 0

Query: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60
           MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK
Sbjct: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60

Query: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120
           YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE
Sbjct: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120

Query: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNNIV 180
           YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNNIV
Sbjct: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNNIV 180

Query: 181 SRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCFK 240
           SRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGF+WDLKNCFK
Sbjct: 181 SRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFHWDLKNCFK 240

Query: 241 TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI 300
           TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI
Sbjct: 241 TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI 300

Query: 301 VLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC 360
           VLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDI+KMEYMQC
Sbjct: 301 VLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIKKMEYMQC 360

Query: 361 VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPE 420
           VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQ WDNPNEFIPE
Sbjct: 361 VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQFWDNPNEFIPE 420

Query: 421 RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL 480
           RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL
Sbjct: 421 RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL 480

Query: 481 DVEEANGLTVRKKKPLHLNPIPYVVSN 507
           DVEEANGLTVRKKKPLHLNPIPYVVSN
Sbjct: 481 DVEEANGLTVRKKKPLHLNPIPYVVSN 507

BLAST of Cucsat.G14243 vs. NCBI nr
Match: XP_031739470.1 (cytochrome P450 71A1-like [Cucumis sativus] >KAE8652604.1 hypothetical protein Csa_013720 [Cucumis sativus])

HSP 1 Score: 1004 bits (2596), Expect = 0.0
Identity = 495/507 (97.63%), Postives = 502/507 (99.01%), Query Frame = 0

Query: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60
           MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK
Sbjct: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60

Query: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120
           YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE
Sbjct: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120

Query: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNNIV 180
           YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSD SLDLGHLFF+TSNNIV
Sbjct: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDHSLDLGHLFFQTSNNIV 180

Query: 181 SRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCFK 240
           S+CVMGEKFE+EDGKSRFEEISRK MVI+TAFC EDFFPSFGWIIDVIRGFNWDLKNCFK
Sbjct: 181 SKCVMGEKFEDEDGKSRFEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFNWDLKNCFK 240

Query: 241 TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI 300
           TLDE FSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDY FSLDNLKAI
Sbjct: 241 TLDELFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYPFSLDNLKAI 300

Query: 301 VLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC 360
           VLDMF+GGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDI+KMEYMQC
Sbjct: 301 VLDMFIGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIKKMEYMQC 360

Query: 361 VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPE 420
           VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPE
Sbjct: 361 VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPE 420

Query: 421 RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL 480
           RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL
Sbjct: 421 RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL 480

Query: 481 DVEEANGLTVRKKKPLHLNPIPYVVSN 507
           DVEEANGLTVRKKKPLHL+PIPYVVSN
Sbjct: 481 DVEEANGLTVRKKKPLHLSPIPYVVSN 507

BLAST of Cucsat.G14243 vs. NCBI nr
Match: XP_011651837.2 (cytochrome P450 71A1 [Cucumis sativus])

HSP 1 Score: 971 bits (2511), Expect = 0.0
Identity = 478/507 (94.28%), Postives = 492/507 (97.04%), Query Frame = 0

Query: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60
           MDPISSLLFFI IFLL LKLFFFKPKTPNFPPSP +LPLIGNLHQLGSLPHQSLATLSQ+
Sbjct: 1   MDPISSLLFFISIFLLLLKLFFFKPKTPNFPPSPPKLPLIGNLHQLGSLPHQSLATLSQE 60

Query: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120
           YGPLMLLKLGQAPVL+ISS K+AEQVMKTHDLVFSNRPQTTAAKTLLYGCQD+GFAPYGE
Sbjct: 61  YGPLMLLKLGQAPVLIISSVKIAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDVGFAPYGE 120

Query: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNNIV 180
           YWRQARKIC LELFSVKRVESFQYVRDEEIDSL+NKIRK GSD SLDLGHLFF+TSNNIV
Sbjct: 121 YWRQARKICVLELFSVKRVESFQYVRDEEIDSLINKIRKIGSDQSLDLGHLFFQTSNNIV 180

Query: 181 SRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCFK 240
           SRCV+GEKFE+EDGKSRFEEISRK MVI+TAFC EDFFPSFGWIIDVIRGF+WDLKNCFK
Sbjct: 181 SRCVLGEKFEDEDGKSRFEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFHWDLKNCFK 240

Query: 241 TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI 300
           TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI
Sbjct: 241 TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI 300

Query: 301 VLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC 360
           VLDMFVGGSD+TATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC
Sbjct: 301 VLDMFVGGSDSTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC 360

Query: 361 VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPE 420
           VIKESLRLHPPVPLL+PRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQ W NPNEFIPE
Sbjct: 361 VIKESLRLHPPVPLLLPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQFWVNPNEFIPE 420

Query: 421 RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL 480
           RFMDKTNSADYKGQN EFIPFGSGRRKCPGLSF IASFEF LANILYWFDWKLPDGCESL
Sbjct: 421 RFMDKTNSADYKGQNLEFIPFGSGRRKCPGLSFAIASFEFVLANILYWFDWKLPDGCESL 480

Query: 481 DVEEANGLTVRKKKPLHLNPIPYVVSN 507
           DVEE+NGLTVRKKK LHLNPIPYVVSN
Sbjct: 481 DVEESNGLTVRKKKSLHLNPIPYVVSN 507

BLAST of Cucsat.G14243 vs. NCBI nr
Match: KAE8652601.1 (hypothetical protein Csa_013149, partial [Cucumis sativus])

HSP 1 Score: 971 bits (2511), Expect = 0.0
Identity = 478/507 (94.28%), Postives = 492/507 (97.04%), Query Frame = 0

Query: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60
           MDPISSLLFFI IFLL LKLFFFKPKTPNFPPSP +LPLIGNLHQLGSLPHQSLATLSQ+
Sbjct: 9   MDPISSLLFFISIFLLLLKLFFFKPKTPNFPPSPPKLPLIGNLHQLGSLPHQSLATLSQE 68

Query: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120
           YGPLMLLKLGQAPVL+ISS K+AEQVMKTHDLVFSNRPQTTAAKTLLYGCQD+GFAPYGE
Sbjct: 69  YGPLMLLKLGQAPVLIISSVKIAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDVGFAPYGE 128

Query: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNNIV 180
           YWRQARKIC LELFSVKRVESFQYVRDEEIDSL+NKIRK GSD SLDLGHLFF+TSNNIV
Sbjct: 129 YWRQARKICVLELFSVKRVESFQYVRDEEIDSLINKIRKIGSDQSLDLGHLFFQTSNNIV 188

Query: 181 SRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCFK 240
           SRCV+GEKFE+EDGKSRFEEISRK MVI+TAFC EDFFPSFGWIIDVIRGF+WDLKNCFK
Sbjct: 189 SRCVLGEKFEDEDGKSRFEEISRKAMVIVTAFCVEDFFPSFGWIIDVIRGFHWDLKNCFK 248

Query: 241 TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI 300
           TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI
Sbjct: 249 TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI 308

Query: 301 VLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC 360
           VLDMFVGGSD+TATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC
Sbjct: 309 VLDMFVGGSDSTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC 368

Query: 361 VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPE 420
           VIKESLRLHPPVPLL+PRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQ W NPNEFIPE
Sbjct: 369 VIKESLRLHPPVPLLLPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQFWVNPNEFIPE 428

Query: 421 RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL 480
           RFMDKTNSADYKGQN EFIPFGSGRRKCPGLSF IASFEF LANILYWFDWKLPDGCESL
Sbjct: 429 RFMDKTNSADYKGQNLEFIPFGSGRRKCPGLSFAIASFEFVLANILYWFDWKLPDGCESL 488

Query: 481 DVEEANGLTVRKKKPLHLNPIPYVVSN 507
           DVEE+NGLTVRKKK LHLNPIPYVVSN
Sbjct: 489 DVEESNGLTVRKKKSLHLNPIPYVVSN 515

BLAST of Cucsat.G14243 vs. NCBI nr
Match: XP_031738835.1 (cytochrome P450 71A1-like isoform X1 [Cucumis sativus])

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 458/496 (92.34%), Postives = 475/496 (95.77%), Query Frame = 0

Query: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60
           MDPISS LFFIFI LL LKLFFFKPKTPNFPPSP +LPLIGNLHQLGSLPHQSLATLSQK
Sbjct: 1   MDPISSSLFFIFIILLLLKLFFFKPKTPNFPPSPPKLPLIGNLHQLGSLPHQSLATLSQK 60

Query: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120
           YGPLMLLKLGQAPVLVISSAKMA+QVMKTHDLVFS+RPQTTA KTLLYG QD+GFAPYGE
Sbjct: 61  YGPLMLLKLGQAPVLVISSAKMAKQVMKTHDLVFSSRPQTTAVKTLLYGGQDVGFAPYGE 120

Query: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDGSLDLGHLFFRTSNNIV 180
           YWRQA+KIC LEL SVKRVESFQYVRDEEID+LVNKIRKSGSD SLDLGHLFF+TSNNIV
Sbjct: 121 YWRQAKKICTLELLSVKRVESFQYVRDEEIDALVNKIRKSGSDQSLDLGHLFFQTSNNIV 180

Query: 181 SRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCFK 240
           +RCVMGEKFE+EDGKSRFEEISRKV+VI+TAFC EDFFPSFGWIIDVIRGF+WDLKNCFK
Sbjct: 181 ARCVMGEKFEDEDGKSRFEEISRKVVVIVTAFCVEDFFPSFGWIIDVIRGFHWDLKNCFK 240

Query: 241 TLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAI 300
           TLDEF SKVVEEHKEKIKRSGG INID+YESKKDFVDIMLQLQQ D+LDYHFSLD+LKAI
Sbjct: 241 TLDEFLSKVVEEHKEKIKRSGGDINIDNYESKKDFVDIMLQLQQGDELDYHFSLDSLKAI 300

Query: 301 VLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC 360
           V DMF+GGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC
Sbjct: 301 VSDMFIGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQC 360

Query: 361 VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPE 420
           VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQ W NPNEFIPE
Sbjct: 361 VIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQFWVNPNEFIPE 420

Query: 421 RFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESL 480
           RFMDKTNSADYKGQNFEFIPFGSGRRKC GLSFGIASFEFALANIL WFDWKLP GCESL
Sbjct: 421 RFMDKTNSADYKGQNFEFIPFGSGRRKCAGLSFGIASFEFALANILCWFDWKLPGGCESL 480

Query: 481 DVEEANGLTVRKKKPL 496
           D+EEANGLTVRKK  L
Sbjct: 481 DIEEANGLTVRKKNQL 496

BLAST of Cucsat.G14243 vs. ExPASy TrEMBL
Match: A0A6J1C631 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV=1)

HSP 1 Score: 738 bits (1904), Expect = 4.72e-264
Identity = 377/509 (74.07%), Postives = 422/509 (82.91%), Query Frame = 0

Query: 3   PISSLLFFIFIFLLSLKLFFFKPK--TPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 62
           P S  +FF F+ LL L     KPK  T NFPPSP +LP+IGNLHQLGSLPHQSLA LS K
Sbjct: 18  PFSLCIFFFFLVLLKLL----KPKNNTKNFPPSPPKLPIIGNLHQLGSLPHQSLAALSDK 77

Query: 63  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 122
           +GPLM LKLGQAP LV+SSA+MA +VMKTHDL FS+RPQTTAAK LLYG +D+GFAPYGE
Sbjct: 78  HGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFSDRPQTTAAKILLYGFKDVGFAPYGE 137

Query: 123 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKS--GSDG--SLDLGHLFFRTS 182
           YWRQARKICALELFS KRVESFQYVRDEE+  LVN+IR+   G DG  S++L  LF  TS
Sbjct: 138 YWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRRGCVGGDGEGSVNLAQLFLSTS 197

Query: 183 NNIVSRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLK 242
           NNIVSRC+MG+KFE+E+GKSRF +ISR+VMV++TAFCF DFFP+  WI DV+RGFN +LK
Sbjct: 198 NNIVSRCMMGDKFEDENGKSRFGDISRRVMVLLTAFCFADFFPALWWI-DVVRGFNGELK 257

Query: 243 NCFKTLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQL-QQDDKLDYHFSLD 302
            CF TLD FFSKVVEEHK K+   G + +    ESK+DF+D MLQL  QDD +DY FSLD
Sbjct: 258 KCFTTLDAFFSKVVEEHKAKMIGGGELCD----ESKRDFMDSMLQLILQDDMVDYRFSLD 317

Query: 303 NLKAIVLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKM 362
           NLKAIVLDMFVGGSDTTATGLEWTMTELMR P +MKKVQEEVR I+G K KIE EDIQ M
Sbjct: 318 NLKAIVLDMFVGGSDTTATGLEWTMTELMRNPSVMKKVQEEVRKIVGNKEKIEMEDIQNM 377

Query: 363 EYMQCVIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPN 422
            YM CVIKESLRLHPPVPLLVPRE + DVEIEGY+IPSKTRVFVNAW I RDP  W+ PN
Sbjct: 378 PYMNCVIKESLRLHPPVPLLVPREAIGDVEIEGYHIPSKTRVFVNAWKIHRDPNIWERPN 437

Query: 423 EFIPERFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPD 482
           EFIPERFM+  NS DYKGQ+F+FIPFGSGRR CPG SFGIASFE+ALAN+LYWFDWKLP 
Sbjct: 438 EFIPERFME--NSIDYKGQDFDFIPFGSGRRICPGKSFGIASFEYALANLLYWFDWKLPH 497

Query: 483 GCESLDVEEANGLTVRKKKPLHLNPIPYV 504
           G + LDVEE NGLTVRKK PLHLNPI Y+
Sbjct: 498 GSKELDVEEENGLTVRKKIPLHLNPISYI 515

BLAST of Cucsat.G14243 vs. ExPASy TrEMBL
Match: A0A6J1C5L5 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV=1)

HSP 1 Score: 737 bits (1903), Expect = 6.95e-264
Identity = 377/504 (74.80%), Postives = 420/504 (83.33%), Query Frame = 0

Query: 2   DPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQKY 61
           D I   L  IF FL+ LKLF  K  T NFPPSP +LP+IGNLHQLGSLPHQSLA LS KY
Sbjct: 17  DLIPFSLCLIFFFLVLLKLFKPKNNTKNFPPSPPKLPIIGNLHQLGSLPHQSLAALSDKY 76

Query: 62  GPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEY 121
           GPLMLLKLG A  LV+SSAKMA ++MKTHDL FSNRPQT  AKTLLYG +D+GFAPYGEY
Sbjct: 77  GPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFSNRPQTMPAKTLLYGFKDVGFAPYGEY 136

Query: 122 WRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDG----SLDLGHLFFRTSN 181
           WRQARKICALELFS KRVESFQYVRDEE+  LVN+IRK   DG    S++L  LF  TSN
Sbjct: 137 WRQARKICALELFSAKRVESFQYVRDEEVSVLVNRIRKGCVDGDGEGSVNLSQLFLSTSN 196

Query: 182 NIVSRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKN 241
           NIVSRC++GEKFE+E+GKSRF + SR+VMV+ITAFCF DFFP+  WI DV+RGFN +LK 
Sbjct: 197 NIVSRCILGEKFEDENGKSRFGDTSRRVMVLITAFCFADFFPALWWI-DVVRGFNGELKK 256

Query: 242 CFKTLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNL 301
            F TLD F SKVVEEHK K+ R+GG  +    ESKKDFV IMLQL Q D +DYHFSL+NL
Sbjct: 257 WFTTLDAFLSKVVEEHKAKM-RAGGESD----ESKKDFVGIMLQLMQSDMVDYHFSLENL 316

Query: 302 KAIVLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEY 361
           K IVLDMFVGG+DTTATGLEWTMTELMR P +M+KVQEEVR  +GKK+KIE EDI+KM Y
Sbjct: 317 KGIVLDMFVGGTDTTATGLEWTMTELMRNPPVMEKVQEEVRKTVGKKAKIEIEDIRKMHY 376

Query: 362 MQCVIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEF 421
           M CVIKESLRLHPPVPLLVPRE ++DVEIEGY+IPSKTRVFVNAW I RDP  W+ PNEF
Sbjct: 377 MNCVIKESLRLHPPVPLLVPREAISDVEIEGYHIPSKTRVFVNAWKIHRDPTIWERPNEF 436

Query: 422 IPERFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGC 481
           IPERFM+  NS DYKGQ+FEFIPFGSGRR CPG+SFGIASFE+ALAN+LYWFDWKLP+G 
Sbjct: 437 IPERFME--NSVDYKGQDFEFIPFGSGRRICPGMSFGIASFEYALANLLYWFDWKLPNGF 496

Query: 482 ESLDVEEANGLTVRKKKPLHLNPI 501
           E LDVEE NGLTVRKK PLHLNPI
Sbjct: 497 E-LDVEEENGLTVRKKNPLHLNPI 511

BLAST of Cucsat.G14243 vs. ExPASy TrEMBL
Match: A0A6J1GRT5 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456925 PE=3 SV=1)

HSP 1 Score: 719 bits (1856), Expect = 8.15e-257
Identity = 369/503 (73.36%), Postives = 413/503 (82.11%), Query Frame = 0

Query: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60
           ++PIS  L   F F+L LKLF  + K PNFPPSP RLP+IGNLHQLG+LPHQS+A LS K
Sbjct: 14  LNPISLSLCLFFFFVLLLKLF--ERKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNK 73

Query: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120
           YGPLMLLKLGQ   LV+SS KMA +VMKTHD+ FSNRPQTTAA  LLY CQD+GFA YGE
Sbjct: 74  YGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAHELLYQCQDIGFAQYGE 133

Query: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDG-SLDLGHLFFRTSNNI 180
           YWRQARK+CALELFSVKRV+SFQYVRDEE+  L+++IRK+   G +++L  LF +TSNNI
Sbjct: 134 YWRQARKVCALELFSVKRVDSFQYVRDEEVGGLMDRIRKACVGGEAVNLSQLFLQTSNNI 193

Query: 181 VSRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCF 240
           VSRCV+GEKFE+E+G SRF    R+ MV+I AFC  D FPS  WI D+IRGFN +LK+C 
Sbjct: 194 VSRCVLGEKFEDENGNSRFGVTVRRAMVLIVAFCVADLFPSLWWI-DIIRGFNKELKDCS 253

Query: 241 KTLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKA 300
           KTLD F SKV+EEHKEKI+   G       ESKKDFVDIMLQLQQDD +DYHFS D+LKA
Sbjct: 254 KTLDTFVSKVIEEHKEKIRDGVGTD-----ESKKDFVDIMLQLQQDDMVDYHFSPDSLKA 313

Query: 301 IVLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQ 360
           ++LDMFVGGSDTTAT LEWTMTELMR P  MKKVQEEVRTI GKKSKIE EDIQKMEYM 
Sbjct: 314 MLLDMFVGGSDTTATTLEWTMTELMRNPSTMKKVQEEVRTITGKKSKIEVEDIQKMEYMH 373

Query: 361 CVIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIP 420
           CVI ESLRLHPPVPLLVPRET ADVEI+GY+IPSKTRVFVNAW IQRD + W NP EFIP
Sbjct: 374 CVINESLRLHPPVPLLVPRETAADVEIDGYHIPSKTRVFVNAWKIQRDLEYWKNPTEFIP 433

Query: 421 ERFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCES 480
           ERFMD   S DYKGQ +EFIPFGSGRRKCPG+SFG+ASFE ALAN+L+WFDWKLP GCE 
Sbjct: 434 ERFMD-NRSVDYKGQCYEFIPFGSGRRKCPGMSFGVASFEQALANLLHWFDWKLPSGCE- 493

Query: 481 LDVEEANGLTVRKKKPLHLNPIP 502
           L VEE  GLTV KK PL LNPIP
Sbjct: 494 LSVEEEGGLTVCKKIPLLLNPIP 506

BLAST of Cucsat.G14243 vs. ExPASy TrEMBL
Match: A0A6J1GRU2 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456926 PE=3 SV=1)

HSP 1 Score: 717 bits (1852), Expect = 2.96e-256
Identity = 369/504 (73.21%), Postives = 418/504 (82.94%), Query Frame = 0

Query: 1   MDPISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQK 60
           ++PISS L   F F++ LKL+  K K PNFPPSP RLP+IGNLHQLG+LPHQS+A LS K
Sbjct: 14  LNPISSSLCLFFFFIVLLKLY--KRKKPNFPPSPPRLPIIGNLHQLGALPHQSMAALSNK 73

Query: 61  YGPLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGE 120
           YGPLMLLKLGQ   LV+SSAKMA +VMKT D+ FSNRPQTTAA  LLY CQD+GF  YGE
Sbjct: 74  YGPLMLLKLGQKHTLVVSSAKMAREVMKTQDIKFSNRPQTTAAYELLYQCQDIGFTQYGE 133

Query: 121 YWRQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKS--GSDGSLDLGHLFFRTSNN 180
           YWRQARK+CALELFS KRV+SFQYVRD+EI  L+++IRK+  G + +++L  LF +TSNN
Sbjct: 134 YWRQARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIRKACVGVE-AVNLSQLFLQTSNN 193

Query: 181 IVSRCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNC 240
           IVSRCV+GEKFE+E+G SRF + +RKVMV++ AFC  D FPS  WI D IRGFN  L++C
Sbjct: 194 IVSRCVLGEKFEDENGNSRFGDAARKVMVLMVAFCVADLFPSLWWI-DTIRGFNKQLEDC 253

Query: 241 FKTLDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLK 300
           FK LD FFSKVVEEHKEKI+   GV+     ESKKDFVDIMLQLQ+D  +DYHFS  NLK
Sbjct: 254 FKRLDTFFSKVVEEHKEKIR--DGVLTD---ESKKDFVDIMLQLQRDGMVDYHFSPINLK 313

Query: 301 AIVLDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYM 360
           AIVLDMFVGGSDTTAT LEWTMTELMR P +MKK QEE+RTIIGKKSKIEAEDIQKMEYM
Sbjct: 314 AIVLDMFVGGSDTTATVLEWTMTELMRNPAVMKKAQEEIRTIIGKKSKIEAEDIQKMEYM 373

Query: 361 QCVIKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFI 420
             VI ESLRLHPP+PLLVPRET  DVEI+ Y+IPSKTRVFVNAW IQRDP+ W NPNEFI
Sbjct: 374 HRVINESLRLHPPIPLLVPRETTTDVEIDSYHIPSKTRVFVNAWKIQRDPENWHNPNEFI 433

Query: 421 PERFMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCE 480
           PERFMD  NS DYKGQ++EFIPFGSGRRKCPGLSFG ASFE ALAN+L+WFDWKLP  C 
Sbjct: 434 PERFMD-NNSIDYKGQDYEFIPFGSGRRKCPGLSFGAASFEHALANLLHWFDWKLPSDC- 493

Query: 481 SLDVEEANGLTVRKKKPLHLNPIP 502
           +L VEE NGLTVRKK PL LNP+P
Sbjct: 494 ALSVEEENGLTVRKKTPLLLNPMP 506

BLAST of Cucsat.G14243 vs. ExPASy TrEMBL
Match: A0A6J1GU09 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456928 PE=3 SV=1)

HSP 1 Score: 716 bits (1847), Expect = 1.71e-255
Identity = 364/501 (72.65%), Postives = 420/501 (83.83%), Query Frame = 0

Query: 3   PISSLLFFIFIFLLSLKLFFFKPKTPNFPPSPLRLPLIGNLHQLGSLPHQSLATLSQKYG 62
           P S  L  +F F++ LKLF  K K PN PPSP RLP+IGNLHQLG+LPHQS+A LS KYG
Sbjct: 16  PNSFSLCLLFFFIVLLKLF--KRKKPNLPPSPPRLPIIGNLHQLGALPHQSMAALSNKYG 75

Query: 63  PLMLLKLGQAPVLVISSAKMAEQVMKTHDLVFSNRPQTTAAKTLLYGCQDMGFAPYGEYW 122
           PLMLLKLGQ   LV+SS K+A++VMKTHD+ FSNR QTTA+K LLY CQD+ F  YGEYW
Sbjct: 76  PLMLLKLGQKQALVVSSPKIAKEVMKTHDIKFSNRLQTTASKKLLYQCQDIAFVHYGEYW 135

Query: 123 RQARKICALELFSVKRVESFQYVRDEEIDSLVNKIRKSGSDG-SLDLGHLFFRTSNNIVS 182
           RQARK+CALELFSVKRV+SFQYVRD+E+  L+++IRK+   G +++L  LF +TSNNIVS
Sbjct: 136 RQARKVCALELFSVKRVDSFQYVRDDEVGGLMDRIRKASVCGEAVNLSQLFLQTSNNIVS 195

Query: 183 RCVMGEKFENEDGKSRFEEISRKVMVIITAFCFEDFFPSFGWIIDVIRGFNWDLKNCFKT 242
           RCV+GEKFE+E+G SRF + +RK+MV+I AFC  D FPS  WI D+IRGF+ +LK+CFKT
Sbjct: 196 RCVLGEKFEDENGNSRFGDTARKLMVLIVAFCAADLFPSLWWI-DIIRGFDKELKDCFKT 255

Query: 243 LDEFFSKVVEEHKEKIKRSGGVINIDDYESKKDFVDIMLQLQQDDKLDYHFSLDNLKAIV 302
           LD FFSKV+EEHKEKI RSG + +    ESK DF+DIMLQLQQDD +DY+FS DNLKAI+
Sbjct: 256 LDTFFSKVLEEHKEKI-RSGVLTD----ESKMDFLDIMLQLQQDDMVDYNFSSDNLKAII 315

Query: 303 LDMFVGGSDTTATGLEWTMTELMRKPRIMKKVQEEVRTIIGKKSKIEAEDIQKMEYMQCV 362
           LDMFVGGSDTTAT LEWTMTELM  P  MKK QEEVRTIIGKKSKIEAEDIQKMEY+ CV
Sbjct: 316 LDMFVGGSDTTATVLEWTMTELMGNPAAMKKAQEEVRTIIGKKSKIEAEDIQKMEYIHCV 375

Query: 363 IKESLRLHPPVPLLVPRETMADVEIEGYYIPSKTRVFVNAWAIQRDPQCWDNPNEFIPER 422
           IKESLRLHPPVPLLVPRET  DVEI+GY+IPSKTRVFVNAW IQRDP+ W NP EFIPER
Sbjct: 376 IKESLRLHPPVPLLVPRETAEDVEIDGYHIPSKTRVFVNAWKIQRDPEYWKNPTEFIPER 435

Query: 423 FMDKTNSADYKGQNFEFIPFGSGRRKCPGLSFGIASFEFALANILYWFDWKLPDGCESLD 482
           F+D   S DYKGQ++EFIPFGSGRRKCPG+SFG+ASFE ALAN+L+WFDWKLP GCE L 
Sbjct: 436 FID-NRSVDYKGQDYEFIPFGSGRRKCPGMSFGVASFEQALANLLHWFDWKLPSGCE-LS 495

Query: 483 VEEANGLTVRKKKPLHLNPIP 502
           VEE +GLTVRKK PL LNP+P
Sbjct: 496 VEEEDGLTVRKKTPLILNPMP 506