Cucsat.G141 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G141
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	3-hydroxy-3-methylglutaryl coenzyme A synthase
Location	ctg1: 2761706 .. 2764212 (-)
RNA-Seq Expression	Cucsat.G141
Synteny	Cucsat.G141

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGGGGAAAAGGTAAATAAAAAGGAACATGAGTTTACAAAAATATTTGGGAGGAAGTTCATGTAGAGTGCATTCTATTTAAGACCCGCCCCCATGTGAGCATCACCATCTTTCTCTGCTGTTCGCCAGGATTTCCTCTTCTCTTCTCTCTCTCTCAAATTTTTTCATTCTCTCCCATTTCTTCTTCGTTTGAGGTACAATATCCATCACCTTCATACCTTACTTTCATTCTTTATTGCTTTGGATCCCCTTTAATTTCAATTCAAACCCATTAACAGCTTCCAGAAATGGCTAACAATGTCGGAATTCTCGCTATAGATATCTACTTCCCTCCTACCTATGTTCAGCAGGTGAAGATCTCTCCTTTTCATCTCCCCTTTAAACCTTTTTCTCTACTTTCCTGTTTCTGATTATATTTTTTAGTGTATGGACAAATTCGTTATCTGGGGGTTGTTTTTTTTTTTTTTTTTCTTTTTTCTTGTTTCGTTGAATTTTGTGTTTCTCGCTAGTAATTTTGAAGTCGTAGTGTTTTTTTTTCCAGAGATCTTAGATGATTATTCTGTTTTTTCCCTTTTCTCTTTTCTTCCTTGGTTGTTTGCGATTGTGGATTGATTTTGAATTATGCATTTTCACATGTATGTATGATTATAATGTCTGTTTGTGTATGGGTATGAGTTGGGTCCACTTCAACTTTTGAGCTCATTTCTGGGCTAGCCGACCGGACCCAATTGTTTGGTGGCAGCTTTTGAGCTCATATTTTAGCACACATCATGTGCATGTTGTCTTTCATTAACGATTCTCTTGGGTTAAAATTTGTTTGTTGATCTATTTTCTTTTTTCTTTTTTTACTTGGGTCTTCTTGGATCTGCATCAAGTTTTGTTAAGAAGGTTCATTTGATATTTTACTGTAGTATGAATGAAGAATTGCATCTCCATGGTTCTCTACTGGGTTTTTGTACTTACATGTATGGTTGATTGCTACTGAAATTAATATGACTTCTGATTTGGGAACGTTAGGAAGCATTGGAAGCTCATGATGGTGCAAGCAAGGGGAAATACACTATTGGACTTGGACAGGATTGCATGGCATTCTGTACTGAGGTTGAAGATGTTATCTCAATGAGGTAGTGACCATGTTTCATCCTCTCTTGCTAATTTTTGTTGTTGGGCTAAAATCTATGTGGTTTTGTATCTGGACACAGCTGTTAGTTTCAATATCTTTGTTCTGTATTTTAAAGAATCTTCTTGACATCTAAGTTCTTGGATTTTCAGCTTGACAGTTGTCAATTCTCTTCTTGAAAAGTACGGAATTGATCCGAAACAAATTGGCCGGCTAGAAGTAGGCAGTGAGACTGTTATAGATAAAAGCAAATCAATTAAGACTTTCCTGATGCAAATATTTGAGGTAAAAATTTTGGGTCTCAAATGGACTTTATTTCTCTTACACTTTTTTAAAATGAAAAACGAAGGGCCAACAAAAGTGAGGGTTATCAAAACTAAAAGTGGGAATTTTATTTGTCTTCTATTTTATTGAAGTTAGTATCTACCAAAAAGTTTGACATTGAACTATTCTTTGTGACATGTTTGAAAATGATTTTGTAAGTGTTAAAACCACTCAAACAATGCTTTTAATCTATAGTTGGATCAATTGTTTATTAAGTATAATAAGGGCCAATTCATTTGATGCAAGGGTGGACAACATTTTAAGGAAGTTTGCGGGTTAAAAGTTAATTTAAAATAATTGCGATGATTTTCATCAGTGGATCAAATGGAATACACGGTTCATTAACTTATAAGTTAAAGTTCATTTAGAGAAGCCAAAAAACTAGGAGTCTTGTCAACAACTATTTTTTTATTCCTTATTTTCCCCTCTTTTTCATTAGTTAATTTACTGGTAATAGTAGAAAAGTATGGATCACTAACATTACTTATTTTATTTAAGCTCTATAATACTTTAATAAATAACATGCATAATATAGAGAGAAGACAAGGACAGCTGAAGTTTAGTAGTTTTGAAGATGTGAATCTCAATTTTTATCTACTACACTGTTTGAGTGGAATCATTTTCTTGATATTATAAATATTGGTTCATTGATGGGCACTTTACTGAATGTTCTTATCTTTTCATCATCGCAGAAGCATGGAAATACCGATATCGAAGGTGTTGATTCAACCAATGCGTGCTATGGGGGTACAGCTGCTCTATTCAACTGTGTCAACTGGGTAGAGAGCAGCTCCTGGGATGGGCGTTATGGTCTTGTGGTGTGCACAGATAGTGCGGTATGATATAAACTGGTCTATCTTACTCTTATACAAACAGATAGTGCATCTATATAAATGGAAAAAAAAGACATATTTGACTCTGTTCTGTTGAAATATATTATACCATTATTTTCTTGGAATATTTGCTGTCAAATCCTGTATTATTGACTGGTCTATTTTGTTAGGTCTATGCTGAAGGACCAGCTCGTCCTACTGGTGGGGCTGCAGCTATTGCCATGCTGATAGGACCTGATGCTCCTATTGCTTTTGAAAGCAAGTTGAGGGGAAGCCATATGTCTCACGTTTATGATTTTTACAAGCCTAACCTAGCCAGCGAATACCCGGTAATCTTTAACAGAATAAACTGTAATCCATTTATATGACATCATGTTATGCTTCAGTTTCTATTGTACTAGAGTTGTTTTTCGATTGTATAACTTTTTTCCCCATTTTGTTTATCCTGGTTTTAGTAATTTATATAGCTTCTGGCTACATTTACTGAGCCATCTATAACAAAATCCTTGCAGGTTGTTGATGGTAAGCTCTCTCAAACATGTTACCTCATGGCCTTGGATTCCTGCTACAAACAATTATGTCACAAGTACGTTCATAATTCGAATAAATTAATCTTCCTTTGCAGACTCGGTCTTTTATCTTAACATTCCGTTGCACTTTTATTAGGTATGAGAAACTGGAGGGCGGCAAACAATTTTCTCTTTCTAATGCTGACTATTTTGTGTTTCATTCTCCCTATAACAAGGTAACATATGTAAAAAAATTTGATTATGGCTTGTTTAATTACTAAAAACTTATTCAGTATTTCTGTGTTTTCCAAGTAAGAGCTTTGAAAATGTTTACCTAATGATTCAATTTTCTTTTATGTACTGCAGCTTGTGCAGAAGAGCTTTGCTCGATTACTCTTTAATGATTTCAAGAGAAATGCTAGGTATATATGCTACTTCTGCTCAAGAATTCTCACTTTTCAAATTCAAATTCTAAACATCTGGTTGTTTATGGTGGCATGTTAAAGTTTTCTCTGCTTTTTACAGCTCAATAGACGAAGCTGCTAAAGAAAAGCTAGCCCCATTTTCAACCTTGTCTAACGATGAGAGTTACCAGAGTCGGGATCTCGAAAAGGTATTATACCTCATGACAGACATCCTTTTACTGGTCATTTGGTTTTCACTTTTTATTAACTAATGTAATGGATTTGCAGATAACTCAACAGCTTGCAAAGCCTCTTTATGATGCAAAGGTCCAGCCCTCCACTTTGATACCAAAGCAAGTTGGAAACATGTATACTGCCTCTCTCTACGCAGCATTTATATCACTTCTTCACAACAAGAATAAATCACTGGTATATATCATCTCTATCGCCTACATACACAATCTGGCTGCTCTGTTTCACCAGTCTAAATGGCTTGTTTCCATACCAGGTAGGAAATAGGGTGGTATTGTTCTCTTATGGAAGCGGGTCAACTGCAACGATGTTCTCCTTGAAACTCAATGAAGGCCAAAATCCCTTTAGTTTGTCCAACATTTCAGCTATCTTGAACGTGGATAAAAAGTTGAAATCAAGGCACGAGGTAATTATTTCTCAGGTTTTCTATTAACTTGACTATTATGCAGAAGTCTAGGTAGTATATAGATCCGCATCAAAACAATTGTCTCAAATGTAATTGAGAGAGAAATTAATTTTAAGCCGATGCTACAATGCCATTTTTAGCCTGAATGCTATCTGTCCAATAGTTGTCCTATAGTTTGGACAGTTCAAACTTTGGATGAGCTTGCAGAAAAGTTGGACAAGGATGGTGTTCAAATCACCACTAAACTGAAAAGCTTGCTTAAAACTAACCTACAGTTAAATATTTGATTGTTCTTGTATAATCTTTCATTTTTCTCGGTGAAAGCTCGGTTCTTATAAAGAATACTTACCAAGTCAACCTTCATACATCTGTGGTTCTAAGAGCCTTTTGTCTTTAGTTTTTTATTGTTGTGTACTTTAGAAAATCGAACTTAAAGATGGTTTTATTAATCCATGGATTGGGACATTTAATTTTCATGCAGTTGGTACCCGAGAAATTTGTCGAAATAATGCAGCTGATGGAGCATCGGTACGGGGCGAAGGACTTCGTGACGAGCAAGGACTGCAGCCTGCTATCATCAGGCACCTACTATCTTACTGAAGTTGATTCGTTATACAGGAGATTCTACGCCAAGAAGGAAGGCGGAAGTGAAAAGATTGAGAATGGTGTGGTTGCTAATGGCCACTGAAATAATAACAACAAATAAACAAGTGGACTGAAGCCAAATGCTGTGCTCTTCATTTAATTATTGTAAGTGTCAACAAGAATTTTTCTTTATCATAAGTTATTTACAAGCTAAGCTAAGCTATAGAAGACAGTACGTTCACAACTTGTAGTTTTCCGTTCTTTCTTTTTTTTTTCTCCCTTCTTGTTAGGAAAATTGTCTCAATAATTGGTTGCCTTTATTTATTCACAAGATAAAATGTAATGTTGTTGTTGATGATGTAATGTAGAGACTCCTTCCCCCCATTTTTCACTTCCATCGGTAATTCTCATCAAATTTGTTTGTTCATAGAACTGTATCTCACCGGTTTTTGGTTGGAAGATGTCCATGTTTGAGTTAGCTTACGTCGCACATCTCGA

Coding sequence (CDS)

ATGGCTAACAATGTCGGAATTCTCGCTATAGATATCTACTTCCCTCCTACCTATGTTCAGCAGGAAGCATTGGAAGCTCATGATGGTGCAAGCAAGGGGAAATACACTATTGGACTTGGACAGGATTGCATGGCATTCTGTACTGAGGTTGAAGATGTTATCTCAATGAGCTTGACAGTTGTCAATTCTCTTCTTGAAAAGTACGGAATTGATCCGAAACAAATTGGCCGGCTAGAAGTAGGCAGTGAGACTGTTATAGATAAAAGCAAATCAATTAAGACTTTCCTGATGCAAATATTTGAGAAGCATGGAAATACCGATATCGAAGGTGTTGATTCAACCAATGCGTGCTATGGGGGTACAGCTGCTCTATTCAACTGTGTCAACTGGGTAGAGAGCAGCTCCTGGGATGGGCGTTATGGTCTTGTGGTGTGCACAGATAGTGCGGTCTATGCTGAAGGACCAGCTCGTCCTACTGGTGGGGCTGCAGCTATTGCCATGCTGATAGGACCTGATGCTCCTATTGCTTTTGAAAGCAAGTTGAGGGGAAGCCATATGTCTCACGTTTATGATTTTTACAAGCCTAACCTAGCCAGCGAATACCCGGTTGTTGATGGTAAGCTCTCTCAAACATGTTACCTCATGGCCTTGGATTCCTGCTACAAACAATTATGTCACAAGTATGAGAAACTGGAGGGCGGCAAACAATTTTCTCTTTCTAATGCTGACTATTTTGTGTTTCATTCTCCCTATAACAAGCTTGTGCAGAAGAGCTTTGCTCGATTACTCTTTAATGATTTCAAGAGAAATGCTAGCTCAATAGACGAAGCTGCTAAAGAAAAGCTAGCCCCATTTTCAACCTTGTCTAACGATGAGAGTTACCAGAGTCGGGATCTCGAAAAGATAACTCAACAGCTTGCAAAGCCTCTTTATGATGCAAAGGTCCAGCCCTCCACTTTGATACCAAAGCAAGTTGGAAACATGTATACTGCCTCTCTCTACGCAGCATTTATATCACTTCTTCACAACAAGAATAAATCACTGGTAGGAAATAGGGTGGTATTGTTCTCTTATGGAAGCGGGTCAACTGCAACGATGTTCTCCTTGAAACTCAATGAAGGCCAAAATCCCTTTAGTTTGTCCAACATTTCAGCTATCTTGAACGTGGATAAAAAGTTGAAATCAAGGCACGAGTTGGTACCCGAGAAATTTGTCGAAATAATGCAGCTGATGGAGCATCGGTACGGGGCGAAGGACTTCGTGACGAGCAAGGACTGCAGCCTGCTATCATCAGGCACCTACTATCTTACTGAAGTTGATTCGTTATACAGGAGATTCTACGCCAAGAAGGAAGGCGGAAGTGAAAAGATTGAGAATGGTGTGGTTGCTAATGGCCACTGA

Protein sequence

MANNVGILAIDIYFPPTYVQQEALEAHDGASKGKYTIGLGQDCMAFCTEVEDVISMSLTVVNSLLEKYGIDPKQIGRLEVGSETVIDKSKSIKTFLMQIFEKHGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCHKYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFSTLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNKSLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVEIMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH

Homology

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BLAST of Cucsat.G141 vs. ExPASy Swiss-Prot
Match: P54873 (Hydroxymethylglutaryl-CoA synthase OS=Arabidopsis thaliana OX=3702 GN=HMGS PE=1 SV=2)

HSP 1 Score: 461.8 bits (1187), Expect = 5.7e-129
Identity = 229/302 (75.83%), Postives = 264/302 (87.42%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPI FESKLR SHM+HVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYK LC+
Sbjct: 167 MLIGPDAPIVFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCN 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           K+EK+E GK+FS+++ADY VFHSPYNKLVQKSFARLL+NDF RNASSIDEAAKEK  P+S
Sbjct: 227 KFEKIE-GKEFSINDADYIVFHSPYNKLVQKSFARLLYNDFLRNASSIDEAAKEKFTPYS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           +L+ DESYQSRDLEK++QQ+AKP YDAKVQP+TLIPK+VGNMYTASLYAAF SL+H K+ 
Sbjct: 287 SLTLDESYQSRDLEKVSQQIAKPFYDAKVQPTTLIPKEVGNMYTASLYAAFASLIHKKHN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
            L G RVV+FSYGSGSTATMFSL+LN+ + PFS+SNI+++++V  KLK+RHE  PEKFVE
Sbjct: 347 DLAGKRVVMFSYGSGSTATMFSLRLNDNKPPFSISNIASVMDVGGKLKARHEYAPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKD--CSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVAN 300
            M+LMEHRYGAKDFVT+K+    LL+ GTYYL EVDSLYRRFY KK       E+G VAN
Sbjct: 407 TMKLMEHRYGAKDFVTTKEGIIDLLAPGTYYLKEVDSLYRRFYGKKG------EDGSVAN 461

[Close]

BLAST of Cucsat.G141 vs. ExPASy Swiss-Prot
Match: P54961 (Hydroxymethylglutaryl-CoA synthase 1 OS=Blattella germanica OX=6973 GN=HMGCS-1 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.3e-60
Identity = 122/289 (42.21%), Postives = 176/289 (60.90%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           ML+G +AP+ F+  +R SHM H YDFYKP+L+S YP VDGKLS  CYL ALD CY+  C 
Sbjct: 166 MLVGANAPLVFDRGVRSSHMQHAYDFYKPDLSSLYPTVDGKLSIQCYLSALDHCYQLYCS 225

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           K +K + G++F +   D  +FH+PY KLVQKS ARL+ NDF R +          L    
Sbjct: 226 KIQK-QLGEKFDIERLDAVLFHAPYCKLVQKSLARLVLNDFVRASEEERTTKYSSLEALK 285

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN- 180
            +  +++Y  R++EK     +K +++ K +PS L+  QVGNMYT SLY   +SLL +K+ 
Sbjct: 286 GVKLEDTYFDREVEKAVMTYSKNMFEEKTKPSLLLANQVGNMYTPSLYGGLVSLLVSKSA 345

Query: 181 KSLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSN--ISAILNVDKKLKSRHELVPEK 240
           + L G RV LFSYGSG  ++MFSL+++   +  S     +S + ++  +L  RH++ PE+
Sbjct: 346 QELAGKRVALFSYGSGLASSMFSLRISSDASAKSSLQRLVSNLSHIKPQLDLRHKVSPEE 405

Query: 241 FVEIMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEG 287
           F + M+  EH +    +       +L  GT+YL  VDSLYRR Y +  G
Sbjct: 406 FAQTMETREHNHHKAPYTPEGSIDVLFPGTWYLESVDSLYRRSYKQVPG 453

[Close]

BLAST of Cucsat.G141 vs. ExPASy Swiss-Prot
Match: P54872 (Hydroxymethylglutaryl-CoA synthase A OS=Dictyostelium discoideum OX=44689 GN=hgsA PE=1 SV=2)

HSP 1 Score: 232.6 bits (592), Expect = 5.7e-60
Identity = 124/281 (44.13%), Postives = 176/281 (62.63%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGP+API FES LRG HM +VYDFYKP++ SEYP VDGKLS +CY  A+D+CY +   
Sbjct: 169 MLIGPNAPITFESGLRGVHMENVYDFYKPDMDSEYPRVDGKLSISCYFRAIDNCYNRYAK 228

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
            +EK + GK FSL   D+ +FHSPYNKLVQKSF R+L+NDF  N    +++    L  + 
Sbjct: 229 AFEK-KYGKSFSLDQVDFALFHSPYNKLVQKSFGRMLYNDFLNNP---NDSRYASLEAYK 288

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
            +  +++Y    LEK    + K  Y  KV P+TL+ KQ+GN Y  S Y+  +SLL  K+ 
Sbjct: 289 NVKPEDTYFDSVLEKALSAITKNDYATKVAPTTLLAKQLGNTYCGSTYSGLLSLLDEKSN 348

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
            LVG RV+ FSYGSG  A+ FS K+ +      +++I   +++  +L  R  + PE F E
Sbjct: 349 DLVGKRVLTFSYGSGLAASAFSFKVEK-----PINHIVEKVDLKNRLAKRVRVEPEIFTE 408

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFY 282
            + L E R+  K++V S + + +  G++YL+ VD+   R Y
Sbjct: 409 KLSLRETRHNLKNYVPSDETTNMFPGSFYLSSVDNAGIRKY 440

[Close]

BLAST of Cucsat.G141 vs. ExPASy Swiss-Prot
Match: P54868 (Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Homo sapiens OX=9606 GN=HMGCS2 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 9.7e-60
Identity = 124/291 (42.61%), Postives = 183/291 (62.89%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCY----K 60
           MLIGP AP+A E  LRG+HM +VYDFYKPNLASEYP+VDGKLS  CYL ALD CY    K
Sbjct: 216 MLIGPKAPLALERGLRGTHMENVYDFYKPNLASEYPIVDGKLSIQCYLRALDRCYTSYRK 275

Query: 61  QLCHKYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKL 120
           ++ +++++    + F+L +  Y +FH+P+ K+VQKS ARL+FNDF   +S    +  + L
Sbjct: 276 KIQNQWKQAGSDRPFTLDDLQYMIFHTPFCKMVQKSLARLMFNDFLSASSDTQTSLYKGL 335

Query: 121 APFSTLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLL- 180
             F  L  +++Y ++DL+K   + ++ ++D K + S  +    GNMYT+SLY    SLL 
Sbjct: 336 EAFGGLKLEDTYTNKDLDKALLKASQDMFDKKTKASLYLSTHNGNMYTSSLYGCLASLLS 395

Query: 181 HNKNKSLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSN--ISAILNVDKKLKSRHEL 240
           H+  + L G+R+  FSYGSG  A+ FS ++++   P S  +  +S+  ++ K+L SR  +
Sbjct: 396 HHSAQELAGSRIGAFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCV 455

Query: 241 VPEKFVEIMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKK 285
            PE+F EIM   E  Y   +F    D + L  GT+YL  VD  +RR YA++
Sbjct: 456 SPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHRRKYARR 506

[Close]

BLAST of Cucsat.G141 vs. ExPASy Swiss-Prot
Match: P54869 (Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcs2 PE=1 SV=2)

HSP 1 Score: 230.7 bits (587), Expect = 2.2e-59
Identity = 122/290 (42.07%), Postives = 181/290 (62.41%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCY----K 60
           MLIGP AP+  E  LRG+HM + YDFYKPNLASEYP+VDGKLS  CYL ALD CY    K
Sbjct: 216 MLIGPKAPLVLEQGLRGTHMENAYDFYKPNLASEYPLVDGKLSIQCYLRALDRCYAAYRK 275

Query: 61  QLCHKYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKL 120
           ++ +++++    + F+L +  Y +FH+P+ K+VQKS ARL+FNDF  ++S       + L
Sbjct: 276 KIQNQWKQAGNNQPFTLDDVQYMIFHTPFCKMVQKSLARLMFNDFLSSSSDKQNNLYKGL 335

Query: 121 APFSTLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLL- 180
             F  L  +E+Y ++D++K   + +  +++ K + S  +    GNMYT+SLY    SLL 
Sbjct: 336 EAFRGLKLEETYTNKDVDKALLKASLDMFNQKTKASLYLSTNNGNMYTSSLYGCLASLLS 395

Query: 181 HNKNKSLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSN--ISAILNVDKKLKSRHEL 240
           H+  + L G+R+  FSYGSG  A+ FS ++++  +P S     +S++ ++ K+L SR  +
Sbjct: 396 HHSAQELAGSRIGAFSYGSGLAASFFSFRVSKDASPGSPLEKLVSSVSDLPKRLDSRRRM 455

Query: 241 VPEKFVEIMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAK 284
            PE+F EIM   E  Y   +F    D S L  GT+YL  VD ++RR YA+
Sbjct: 456 SPEEFTEIMNQREQFYHKVNFSPPGDTSNLFPGTWYLERVDEMHRRKYAR 505

[Close]

BLAST of Cucsat.G141 vs. NCBI nr
Match: XP_004147967.1 (hydroxymethylglutaryl-CoA synthase [Cucumis sativus] >KGN55947.1 hypothetical protein Csa_009493 [Cucumis sativus])

HSP 1 Score: 599 bits (1544), Expect = 2.72e-213
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS
Sbjct: 227 KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK
Sbjct: 287 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 466

[Close]

BLAST of Cucsat.G141 vs. NCBI nr
Match: XP_008448952.1 (PREDICTED: hydroxymethylglutaryl-CoA synthase [Cucumis melo] >XP_016900662.1 PREDICTED: hydroxymethylglutaryl-CoA synthase [Cucumis melo] >TYK19443.1 hydroxymethylglutaryl-CoA synthase [Cucumis melo var. makuwa])

HSP 1 Score: 583 bits (1504), Expect = 3.21e-207
Identity = 294/300 (98.00%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEG KQFS+SNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSID AAKEKLAPFS
Sbjct: 227 KYEKLEG-KQFSISNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDVAAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
            LSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN 
Sbjct: 287 ALSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLLS+GTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLSTGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 465

[Close]

BLAST of Cucsat.G141 vs. NCBI nr
Match: XP_038903028.1 (hydroxymethylglutaryl-CoA synthase [Benincasa hispida])

HSP 1 Score: 579 bits (1492), Expect = 2.15e-205
Identity = 291/300 (97.00%), Postives = 294/300 (98.00%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEG KQFS+SNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDE AKEKLAPFS
Sbjct: 227 KYEKLEG-KQFSISNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEPAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
            LSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN 
Sbjct: 287 NLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SLVGNRV+LFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLVGNRVILFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLLS GTYYLTEVDSLYRRFYAKK+G SEKIENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLSPGTYYLTEVDSLYRRFYAKKDGESEKIENGVVANGH 465

[Close]

BLAST of Cucsat.G141 vs. NCBI nr
Match: KAG6577575.1 (Hydroxymethylglutaryl-CoA synthase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015627.1 Hydroxymethylglutaryl-CoA synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 566 bits (1458), Expect = 3.21e-200
Identity = 283/300 (94.33%), Postives = 291/300 (97.00%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           ML+GPDAPI FESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLVGPDAPIGFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEG KQFS+S+ADYFVFHSPYNKLVQKSFARLLFNDF RNASSIDE AKEKLAPFS
Sbjct: 227 KYEKLEG-KQFSISDADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           TLSNDE+YQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN 
Sbjct: 287 TLSNDETYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SL GNRVVLFSYGSGSTATMFSLKLNEGQ PFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLTGNRVVLFSYGSGSTATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLL +G+YYLTEVDSLYRRFYAKK+G SEK+ENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465

[Close]

BLAST of Cucsat.G141 vs. NCBI nr
Match: XP_023553213.1 (hydroxymethylglutaryl-CoA synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566 bits (1458), Expect = 3.21e-200
Identity = 283/300 (94.33%), Postives = 291/300 (97.00%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           ML+GPDAPI FESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLVGPDAPIGFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEG KQFS+S+ADYFVFHSPYNKLVQKSFARLLFNDF RNASSIDE AKEKLAPFS
Sbjct: 227 KYEKLEG-KQFSISDADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           TLSNDE+YQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN 
Sbjct: 287 TLSNDETYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SL GNRVVLFSYGSGSTATMFSLKLNEGQ PFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLTGNRVVLFSYGSGSTATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLL +G+YYLTEVDSLYRRFYAKK+G SEK+ENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465

[Close]

BLAST of Cucsat.G141 vs. ExPASy TrEMBL
Match: A0A0A0L5T1 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis sativus OX=3659 GN=Csa_3G038200 PE=3 SV=1)

HSP 1 Score: 599 bits (1544), Expect = 1.32e-213
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS
Sbjct: 227 KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK
Sbjct: 287 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 466

[Close]

BLAST of Cucsat.G141 vs. ExPASy TrEMBL
Match: A0A1S3BKB7 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis melo OX=3656 GN=LOC103490966 PE=3 SV=1)

HSP 1 Score: 583 bits (1504), Expect = 1.56e-207
Identity = 294/300 (98.00%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEG KQFS+SNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSID AAKEKLAPFS
Sbjct: 227 KYEKLEG-KQFSISNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDVAAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
            LSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN 
Sbjct: 287 ALSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLLS+GTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLSTGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 465

[Close]

BLAST of Cucsat.G141 vs. ExPASy TrEMBL
Match: A0A5D3D7H1 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00950 PE=3 SV=1)

HSP 1 Score: 583 bits (1504), Expect = 1.56e-207
Identity = 294/300 (98.00%), Postives = 296/300 (98.67%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEG KQFS+SNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSID AAKEKLAPFS
Sbjct: 227 KYEKLEG-KQFSISNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDVAAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
            LSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN 
Sbjct: 287 ALSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLLS+GTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLSTGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 465

[Close]

BLAST of Cucsat.G141 vs. ExPASy TrEMBL
Match: A0A6J1E6B9 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucurbita moschata OX=3662 GN=LOC111431190 PE=3 SV=1)

HSP 1 Score: 566 bits (1458), Expect = 1.55e-200
Identity = 283/300 (94.33%), Postives = 291/300 (97.00%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           ML+GPDAPI FESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLVGPDAPIGFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEG KQFS+S+ADYFVFHSPYNKLVQKSFARLLFNDF RNASSIDE AKEKLAPFS
Sbjct: 227 KYEKLEG-KQFSISDADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           TLSNDE+YQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKN 
Sbjct: 287 TLSNDETYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SL GNRVVLFSYGSGSTATMFSLKLNEGQ PFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLTGNRVVLFSYGSGSTATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLL +G+YYLTEVDSLYRRFYAKK+G SEK+ENGVVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESEKMENGVVANGH 465

[Close]

BLAST of Cucsat.G141 vs. ExPASy TrEMBL
Match: A0A6J1HPX8 (3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucurbita maxima OX=3661 GN=LOC111465024 PE=3 SV=1)

HSP 1 Score: 561 bits (1447), Expect = 7.32e-199
Identity = 281/300 (93.67%), Postives = 289/300 (96.33%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           ML+GPDAPI FESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH
Sbjct: 167 MLVGPDAPIGFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           KYEKLEG KQFS+S+ADYFVFHSPYNKLVQKSFARLLFNDF RNASSIDE AKEKLAPFS
Sbjct: 227 KYEKLEG-KQFSISDADYFVFHSPYNKLVQKSFARLLFNDFMRNASSIDEVAKEKLAPFS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           TLSNDE+YQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHN+N 
Sbjct: 287 TLSNDETYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNRNN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
           SL GNRVVLFSYGSGSTATMFSLKLNEGQ PFSLSNISAILNVDKKLKSRHELVPEKFVE
Sbjct: 347 SLTGNRVVLFSYGSGSTATMFSLKLNEGQAPFSLSNISAILNVDKKLKSRHELVPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKDCSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVANGH 300
           IMQLMEHRYGAKDFVTSKDCSLL +G+YYLTEVDSLYRRFYAKK+G SE IEN VVANGH
Sbjct: 407 IMQLMEHRYGAKDFVTSKDCSLLPAGSYYLTEVDSLYRRFYAKKDGESENIENSVVANGH 465

[Close]

BLAST of Cucsat.G141 vs. TAIR 10
Match: AT4G11820.1 (hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase )

HSP 1 Score: 461.8 bits (1187), Expect = 4.1e-130
Identity = 229/302 (75.83%), Postives = 264/302 (87.42%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPI FESKLR SHM+HVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYK LC+
Sbjct: 112 MLIGPDAPIVFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCN 171

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           K+EK+E GK+FS+++ADY VFHSPYNKLVQKSFARLL+NDF RNASSIDEAAKEK  P+S
Sbjct: 172 KFEKIE-GKEFSINDADYIVFHSPYNKLVQKSFARLLYNDFLRNASSIDEAAKEKFTPYS 231

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           +L+ DESYQSRDLEK++QQ+AKP YDAKVQP+TLIPK+VGNMYTASLYAAF SL+H K+ 
Sbjct: 232 SLTLDESYQSRDLEKVSQQIAKPFYDAKVQPTTLIPKEVGNMYTASLYAAFASLIHKKHN 291

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
            L G RVV+FSYGSGSTATMFSL+LN+ + PFS+SNI+++++V  KLK+RHE  PEKFVE
Sbjct: 292 DLAGKRVVMFSYGSGSTATMFSLRLNDNKPPFSISNIASVMDVGGKLKARHEYAPEKFVE 351

Query: 241 IMQLMEHRYGAKDFVTSKD--CSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVAN 300
            M+LMEHRYGAKDFVT+K+    LL+ GTYYL EVDSLYRRFY KK       E+G VAN
Sbjct: 352 TMKLMEHRYGAKDFVTTKEGIIDLLAPGTYYLKEVDSLYRRFYGKKG------EDGSVAN 406

[Close]

BLAST of Cucsat.G141 vs. TAIR 10
Match: AT4G11820.2 (hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase )

HSP 1 Score: 461.8 bits (1187), Expect = 4.1e-130
Identity = 229/302 (75.83%), Postives = 264/302 (87.42%), Query Frame = 0


Query: 1   MLIGPDAPIAFESKLRGSHMSHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKQLCH 60
           MLIGPDAPI FESKLR SHM+HVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYK LC+
Sbjct: 167 MLIGPDAPIVFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCN 226

Query: 61  KYEKLEGGKQFSLSNADYFVFHSPYNKLVQKSFARLLFNDFKRNASSIDEAAKEKLAPFS 120
           K+EK+E GK+FS+++ADY VFHSPYNKLVQKSFARLL+NDF RNASSIDEAAKEK  P+S
Sbjct: 227 KFEKIE-GKEFSINDADYIVFHSPYNKLVQKSFARLLYNDFLRNASSIDEAAKEKFTPYS 286

Query: 121 TLSNDESYQSRDLEKITQQLAKPLYDAKVQPSTLIPKQVGNMYTASLYAAFISLLHNKNK 180
           +L+ DESYQSRDLEK++QQ+AKP YDAKVQP+TLIPK+VGNMYTASLYAAF SL+H K+ 
Sbjct: 287 SLTLDESYQSRDLEKVSQQIAKPFYDAKVQPTTLIPKEVGNMYTASLYAAFASLIHKKHN 346

Query: 181 SLVGNRVVLFSYGSGSTATMFSLKLNEGQNPFSLSNISAILNVDKKLKSRHELVPEKFVE 240
            L G RVV+FSYGSGSTATMFSL+LN+ + PFS+SNI+++++V  KLK+RHE  PEKFVE
Sbjct: 347 DLAGKRVVMFSYGSGSTATMFSLRLNDNKPPFSISNIASVMDVGGKLKARHEYAPEKFVE 406

Query: 241 IMQLMEHRYGAKDFVTSKD--CSLLSSGTYYLTEVDSLYRRFYAKKEGGSEKIENGVVAN 300
            M+LMEHRYGAKDFVT+K+    LL+ GTYYL EVDSLYRRFY KK       E+G VAN
Sbjct: 407 TMKLMEHRYGAKDFVTTKEGIIDLLAPGTYYLKEVDSLYRRFYGKKG------EDGSVAN 461

The following BLAST results are available for this feature:

BLAST of Cucsat.G141 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P54873	5.7e-129	75.83	Hydroxymethylglutaryl-CoA synthase OS=Arabidopsis thaliana OX=3702 GN=HMGS PE=1 ...	[more]
P54961	3.3e-60	42.21	Hydroxymethylglutaryl-CoA synthase 1 OS=Blattella germanica OX=6973 GN=HMGCS-1 P...	[more]
P54872	5.7e-60	44.13	Hydroxymethylglutaryl-CoA synthase A OS=Dictyostelium discoideum OX=44689 GN=hgs...	[more]
P54868	9.7e-60	42.61	Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Homo sapiens OX=9606 GN=HMG...	[more]
P54869	2.2e-59	42.07	Hydroxymethylglutaryl-CoA synthase, mitochondrial OS=Mus musculus OX=10090 GN=Hm...	[more]

BLAST of Cucsat.G141 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147967.1	2.72e-213	100.00	hydroxymethylglutaryl-CoA synthase [Cucumis sativus] >KGN55947.1 hypothetical pr...	[more]
XP_008448952.1	3.21e-207	98.00	PREDICTED: hydroxymethylglutaryl-CoA synthase [Cucumis melo] >XP_016900662.1 PRE...	[more]
XP_038903028.1	2.15e-205	97.00	hydroxymethylglutaryl-CoA synthase [Benincasa hispida]	[more]
KAG6577575.1	3.21e-200	94.33	Hydroxymethylglutaryl-CoA synthase, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_023553213.1	3.21e-200	94.33	hydroxymethylglutaryl-CoA synthase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G141 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5T1	1.32e-213	100.00	3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis sativus OX=3659 GN=Csa...	[more]
A0A1S3BKB7	1.56e-207	98.00	3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A5D3D7H1	1.56e-207	98.00	3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucumis melo var. makuwa OX=11...	[more]
A0A6J1E6B9	1.55e-200	94.33	3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1HPX8	7.32e-199	93.67	3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Cucsat.G141 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G11820.1	4.1e-130	75.83	hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutar...	[more]
AT4G11820.2	4.1e-130	75.83	hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutar...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010122	Hydroxymethylglutaryl-CoA synthase, eukaryotic	TIGRFAM	TIGR01833	TIGR01833	coord: 48..392 e-value: 1.8E-182 score: 605.4
IPR013746	Hydroxymethylglutaryl-coenzyme A synthase, C-terminal domain	PFAM	PF08540	HMG_CoA_synt_C	coord: 221..394 e-value: 4.2E-72 score: 243.1
IPR013528	Hydroxymethylglutaryl-coenzyme A synthase, N-terminal	PFAM	PF01154	HMG_CoA_synt_N	coord: 49..220 e-value: 3.1E-82 score: 274.7
IPR016039	Thiolase-like	GENE3D	3.40.47.10		coord: 47..400 e-value: 1.9E-154 score: 516.9
IPR016039	Thiolase-like	SUPERFAMILY	53901	Thiolase-like	coord: 50..218
IPR016039	Thiolase-like	SUPERFAMILY	53901	Thiolase-like	coord: 221..390
None	No IPR available	PANTHER	PTHR43323	3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE	coord: 47..391
None	No IPR available	CDD	cd00827	init_cond_enzymes	coord: 50..389 e-value: 1.1295E-77 score: 241.953
IPR000590	Hydroxymethylglutaryl-coenzyme A synthase, active site	PROSITE	PS01226	HMG_COA_SYNTHASE	coord: 151..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G141.T1	Cucsat.G141.T1	mRNA
Cucsat.G141.T2	Cucsat.G141.T2	mRNA
Cucsat.G141.T5	Cucsat.G141.T5	mRNA
Cucsat.G141.T6	Cucsat.G141.T6	mRNA
Cucsat.G141.T11	Cucsat.G141.T11	mRNA
Cucsat.G141.T10	Cucsat.G141.T10	mRNA
Cucsat.G141.T9	Cucsat.G141.T9	mRNA
Cucsat.G141.T3	Cucsat.G141.T3	mRNA
Cucsat.G141.T4	Cucsat.G141.T4	mRNA
Cucsat.G141.T7	Cucsat.G141.T7	mRNA
Cucsat.G141.T8	Cucsat.G141.T8	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006084	acetyl-CoA metabolic process
biological_process	GO:0010142	farnesyl diphosphate biosynthetic process, mevalonate pathway
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008299	isoprenoid biosynthetic process
molecular_function	GO:0004421	hydroxymethylglutaryl-CoA synthase activity
molecular_function	GO:0016746	acyltransferase activity