Cucsat.G13821 (gene) Cucumber (B10) v3

Overview
NameCucsat.G13821
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionHMA domain-containing protein
Locationctg184: 964230 .. 966727 (+)
RNA-Seq ExpressionCucsat.G13821
SyntenyCucsat.G13821
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAAAACAACGGCTTCATTCTTCTCTTTTTCTGAACCACACATATAGTGAAGTTCATACCTTTTTATGTGAGAGATAAAAGGAAATACCAAAAAACCATTTTATCCAAACACACACAAAACTCTCATCTCATCATAACAATCATGAAAACCATCGATTTCTTCTGCGCTTCCCAAGCCTCCACCGCCGTCGACCAACCTTCATCTTCCCCCGCCGGAAGATTCATCGACCGCCACAACCCCATCATCGCTGACGCCAGACGATCAAACGTCACTTCTAGAACAACTAATTTCCCCAACCCTCCATGTTCCTCCCAATACTCCCCCATTAATCCTCTCCCTTACCATCAACTCCATGCCGCCGCCGCCGCCGCCTCCCCTAATGTCGCCGGGGACCAGATCCGGCCGTCCGGCGTCTCCGGAAATCATAAGGATTTGAAGATGAAGAAGAAGAAGAAGAAGAGTTCATCCATTATTACAACTGACTTTGTAAGGTTTGTTCCTTCCTTCCTAATTTCAAACTCTTATCCATTCCCTTTAATTCATTCATTCTCATTTTTATTCATATTCATTTTATTGCCATTTTTATTCATATACATATCTAAACTATTTGAGTATATTTTTACTTTTTAGTGCAAATGAGATGATAGAGATTTGTAATCAACCCTATGATAATATTTTTCGTATAATTTGGAATAATCAATAGACGATACTTTGATTCATAGTGAAACTATTTTATTGATTTCATTCCTAGAAAATTTAACAGATTAAATTTAAAGTTTTTGTTTATGGAAAAAGAAATGGAAGCATTTATTATGAATTGGTATGGGAAATGAAATTAATTAATTGAGAAATAATAATAATGTAGTTTGACCACTCAGGTGGAGCTGTGCAAAGCCCAGTGATTTGGCTACGCCTCCTGGGTCGATGCGATACCTTTTGAACGACAAGTCGGTTCCTGATGGTTCCATGGATCGGATTCCCACGCCTATCCCAATTAACAAAAAACCAACCTTCATCTAACCCACAAGATCCACACCATTCTAAACCAACGCCCCAGATTTCTTCCCAGGACGACTCCAATAAATCTCCACCTTCCAATCAGGTTACTACTTTTCATCCAATTATTCATAACAACAACAAAAATTCAATTCTGTCCTCAGTAATTATTATAAATGAAAAAGTACAGAATTTCAAAATCTATTTTTTTTTTCATGTTTTATAAATATTTTGGTATGGGGTCTACCCAAAAGAAAATTTGGATAGTTATATCAAATTTGCTATTTTTATAATTCTCTTTGATATATATATATATATTAATAAATTATTCATATACTGGTATCTTTCTTTAAATTCATGTAATAGTAGAATATAAAATGTAATTATAATATATCAATTATTAGTTATACAATATATTATAGATTTATTAAATAAAAAAGAAGAAATTTGTCCAAAAAAAATTAAAAAATTGACAAAATATTTATAATGGGTAATAAATTATGTCTTTTGCAATTAAAAAAATAGGACTTAAGGATGCAAACTCTAAATATTTATTAAAAACTTTCAAAGTTAGTTTTTTAAAAAAATAATAAAAAAATATAAGCTATTTACACACAAAAAAAACTATTTATACTATATGACAAAAAATTCATTAAACAACAAAAACTCACTGTATTTTGGTGAAATTAGTTTGTCCATTTTGCAATTTTTTATAATTTTTCTTTAAAATTTTAGATATTAAATTGTATATAAAATTTTATTTTTTACGTTTTGTATTTTTTTCGAGGAGAAATTGTAAAAAGTTAATTTACATAGAAAAAAAATGTAAAATTTTGTTTGTAAATAATTTATCAAAATTTTATATTTTTGAAAAATGTATAGAAAAAACAACTTTTGCATTGTAAGATAATTATAGTAGAATGATTTGGGAATATTATTGTGAAAAAGAAAAAAGGTAATATTTGTGTATGTGTAAAATAATCGGATGAAATTCAATAATAATAATAAATAAATAAAAATAATGAATTAATAAGAAATATTATTGTCAGGTTGTTGTTTTGAGGGTATCATTGCATTGCAGAGGCTGTGAAGGAAAACTTAGGAAACATCTCTCCAAAATGGAAGGTATGGCTAACCCTAACCCACCATACATACATACATATATATATATATATCTTTTGATAGGCCTGACTTCAATGTTGATAAGATTAAAAAAATAAAGTTATTTAAAACATCAAGAGAATCAATTTAATTTTAAGTTGATATTTGTAAACAGTCCTTCTTTTTTGTCCTTTCTAAAAGCAACAAAAGTAATAAAATGGAGTTTACTCTTTCTTATGAAAACTCTCTGGTCCCCCAGAAAAAAAGATATAACTTTATGTATTGATTTTCATTCATTTTTAACCTTTTCAAATATTCGAAATTATACCATTTTAGTCCATATTTTGTGTTGATTGAATGAACTATCAGTGATTTGTAAATGTAGGAGTGAACTCATTCAACATAGATTTTGCGGCAAAAAAGGTGACGATAATGGGAAATATAACGCCACAGGGTATGTTGGAAAGTGTATCCAAGGTTAAAAATGCCCAATTTTGGCCATATGCAGACCCAACTCCAACTCCAAACCCTAATCTTAATCAAAACCATCACCCAAATGTATTGAAAAAAACCTAATAAAATTTTTCTTTTCTAGCTTTTTTTTTTTTTAATTCTTCTAAAAATATTAAACGTATGTTTATTGTGACAGTTTGATTATTATTATATATGAAAATGTTATTTACTTGAAAATCTAAAAACAAAATCATTACGAAACCAATAATATGGTGAATAATGTATATTGTTTGAGTAGAAAGAATCATTTGAGTGAGTTTGTTGGTTGGTTTTGTTTAAAAGCTTAGATTAGAAATTTATGAATTAGGAAATAAAAACCCCATTGTTAAAAGGGGATAACTTAAATATGTTTTATTGGAATTTTAATTCCTTGTTTTGGTGCAATTA

Coding sequence (CDS)

ATGGTTCCATGGATCGGATTCCCACGCCTATCCCAATTAACAAAAAACCAACCTTCATCTAACCCACAAGATCCACACCATTCTAAACCAACGCCCCAGATTTCTTCCCAGGACGACTCCAATAAATCTCCACCTTCCAATCAGGTTGTTGTTTTGAGGGTATCATTGCATTGCAGAGGCTGTGAAGGAAAACTTAGGAAACATCTCTCCAAAATGGAAGGTATGGCTAACCCTAACCCACCATACATACATACATATATATATATATATCTTTTGATAGGCCTGACTTCAATGTTGATAAGATTAAAAAAATAA

Protein sequence

MVPWIGFPRLSQLTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKMEGMANPNPPYIHTYIYIYLLIGLTSMLIRLKK
Homology
[Close]
BLAST of Cucsat.G13821 vs. ExPASy Swiss-Prot
Match: Q8LDS4 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAKR1 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 3.8e-06
Identity = 28/56 (50.00%), Postives = 36/56 (64.29%), Query Frame = 0 

Query: 18  PSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKME 74
           P   P  P  S P+P         K+  S+QVVVLRVSLHC+GC GK++KHLSK++
Sbjct: 227 PPPPPSPPQSSPPSP-------PEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLK 275

[Close]
BLAST of Cucsat.G13821 vs. ExPASy Swiss-Prot
Match: Q58FZ0 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAKR2 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 8.6e-06
Identity = 21/26 (80.77%), Postives = 26/26 (100.00%), Query Frame = 0 

Query: 48  QVVVLRVSLHCRGCEGKLRKHLSKME 74
           QVVVL+VSLHCRGCEGK+RKHL++M+
Sbjct: 181 QVVVLKVSLHCRGCEGKVRKHLARMQ 206

[Close]
BLAST of Cucsat.G13821 vs. ExPASy Swiss-Prot
Match: Q8RXH8 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAKR3 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.3e-05
Identity = 23/30 (76.67%), Postives = 28/30 (93.33%), Query Frame = 0 

Query: 46  SNQVVVLRVSL--HCRGCEGKLRKHLSKME 74
           S+QVVVLRVSL  HCRGC+GK++KHLSKM+
Sbjct: 166 SDQVVVLRVSLHCHCRGCQGKVKKHLSKMQ 195

[Close]
BLAST of Cucsat.G13821 vs. NCBI nr
Match: XP_031739629.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3 isoform X2 [Cucumis sativus])

HSP 1 Score: 204 bits (520), Expect = 4.36e-64
Identity = 96/104 (92.31%), Postives = 98/104 (94.23%), Query Frame = 0 

Query: 7   FPRLSQLTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR 66
            P    + KNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR
Sbjct: 150 IPTPIPINKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR 209

Query: 67  KHLSKMEVICKCRSELIQHRFCGKKGDDNGKYNATGYVGKCIQG 110
           KHLSKMEVICKCRSELIQHRFCGKKGDDNGKYNATGYVGKCI+G
Sbjct: 210 KHLSKMEVICKCRSELIQHRFCGKKGDDNGKYNATGYVGKCIEG 253

[Close]
BLAST of Cucsat.G13821 vs. NCBI nr
Match: XP_011653614.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3 isoform X1 [Cucumis sativus] >KGN54216.1 hypothetical protein Csa_017964 [Cucumis sativus])

HSP 1 Score: 124 bits (312), Expect = 2.20e-32
Identity = 60/69 (86.96%), Postives = 62/69 (89.86%), Query Frame = 0 

Query: 7   FPRLSQLTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR 66
            P    + KNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR
Sbjct: 150 IPTPIPINKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR 209

Query: 67  KHLSKMEVI 75
           KHLSKME +
Sbjct: 210 KHLSKMEGV 218

[Close]
BLAST of Cucsat.G13821 vs. NCBI nr
Match: XP_008447442.1 (PREDICTED: uncharacterized protein LOC103489885 [Cucumis melo] >KAA0047336.1 HMA domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.1 bits (235), Expect = 5.27e-21
Identity = 47/63 (74.60%), Postives = 51/63 (80.95%), Query Frame = 0 

Query: 13  LTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM 72
           + KN   S+ Q PH  +PTPQISS D  NKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM
Sbjct: 156 IHKNLTPSDQQHPHQPQPTPQISSDDHCNKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM 215

Query: 73  EVI 75
           E +
Sbjct: 216 EGV 218

[Close]
BLAST of Cucsat.G13821 vs. NCBI nr
Match: KAG6603681.1 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 86.7 bits (213), Expect = 7.61e-18
Identity = 43/66 (65.15%), Postives = 49/66 (74.24%), Query Frame = 0 

Query: 10  LSQLTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHL 69
           LS  T N+P S  ++   SKPT +I  +DD   SPPSN VVVLRVSLHCRGCEGKLRKHL
Sbjct: 148 LSSQTNNKPDSGTENRDESKPTAEILLEDDCKSSPPSNHVVVLRVSLHCRGCEGKLRKHL 207

Query: 70  SKMEVI 75
           SKME +
Sbjct: 208 SKMEGV 213

[Close]
BLAST of Cucsat.G13821 vs. NCBI nr
Match: KAG7033858.1 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 86.7 bits (213), Expect = 8.10e-18
Identity = 43/66 (65.15%), Postives = 49/66 (74.24%), Query Frame = 0 

Query: 10  LSQLTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHL 69
           LS  T N+P S  ++   SKPT +I  +DD   SPPSN VVVLRVSLHCRGCEGKLRKHL
Sbjct: 152 LSSQTNNKPDSGTENRDESKPTAEILLEDDCKSSPPSNHVVVLRVSLHCRGCEGKLRKHL 211

Query: 70  SKMEVI 75
           SKME +
Sbjct: 212 SKMEGV 217

[Close]
BLAST of Cucsat.G13821 vs. ExPASy TrEMBL
Match: A0A0A0L239 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G293260 PE=4 SV=1)

HSP 1 Score: 124 bits (312), Expect = 1.07e-32
Identity = 60/69 (86.96%), Postives = 62/69 (89.86%), Query Frame = 0 

Query: 7   FPRLSQLTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR 66
            P    + KNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR
Sbjct: 150 IPTPIPINKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLR 209

Query: 67  KHLSKMEVI 75
           KHLSKME +
Sbjct: 210 KHLSKMEGV 218

[Close]
BLAST of Cucsat.G13821 vs. ExPASy TrEMBL
Match: A0A5A7TZF0 (HMA domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G001570 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.55e-21
Identity = 47/63 (74.60%), Postives = 51/63 (80.95%), Query Frame = 0 

Query: 13  LTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM 72
           + KN   S+ Q PH  +PTPQISS D  NKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM
Sbjct: 156 IHKNLTPSDQQHPHQPQPTPQISSDDHCNKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM 215

Query: 73  EVI 75
           E +
Sbjct: 216 EGV 218

[Close]
BLAST of Cucsat.G13821 vs. ExPASy TrEMBL
Match: A0A1S3BIC7 (uncharacterized protein LOC103489885 OS=Cucumis melo OX=3656 GN=LOC103489885 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.55e-21
Identity = 47/63 (74.60%), Postives = 51/63 (80.95%), Query Frame = 0 

Query: 13  LTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM 72
           + KN   S+ Q PH  +PTPQISS D  NKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM
Sbjct: 156 IHKNLTPSDQQHPHQPQPTPQISSDDHCNKSPPSNQVVVLRVSLHCRGCEGKLRKHLSKM 215

Query: 73  EVI 75
           E +
Sbjct: 216 EGV 218

[Close]
BLAST of Cucsat.G13821 vs. ExPASy TrEMBL
Match: A0A6J1ILS7 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like OS=Cucurbita maxima OX=3661 GN=LOC111477619 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.29e-18
Identity = 43/66 (65.15%), Postives = 49/66 (74.24%), Query Frame = 0 

Query: 10  LSQLTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHL 69
           LS  T N+P S  ++   SKPT +I  +DD   SPPSN VVVLRVSLHCRGCEGKLRKHL
Sbjct: 158 LSSQTNNKPDSGTENRDESKPTAEILLEDDCKSSPPSNHVVVLRVSLHCRGCEGKLRKHL 217

Query: 70  SKMEVI 75
           SKME +
Sbjct: 218 SKMEGV 223

[Close]
BLAST of Cucsat.G13821 vs. ExPASy TrEMBL
Match: A0A6J1GEB0 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like OS=Cucurbita moschata OX=3662 GN=LOC111453393 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.85e-17
Identity = 42/66 (63.64%), Postives = 48/66 (72.73%), Query Frame = 0 

Query: 10  LSQLTKNQPSSNPQDPHHSKPTPQISSQDDSNKSPPSNQVVVLRVSLHCRGCEGKLRKHL 69
           LS  T N+P S  ++   SKPT +I  +DD   SPP N VVVLRVSLHCRGCEGKLRKHL
Sbjct: 150 LSSQTNNKPDSGTENRDESKPTAEILLEDDCKSSPPFNHVVVLRVSLHCRGCEGKLRKHL 209

Query: 70  SKMEVI 75
           SKME +
Sbjct: 210 SKMEGV 215

The following BLAST results are available for this feature:
BLAST of Cucsat.G13821 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8LDS43.8e-0650.00Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
Q58FZ08.6e-0680.77Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
Q8RXH83.3e-0576.67Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
BLAST of Cucsat.G13821 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_031739629.14.36e-6492.31protein SODIUM POTASSIUM ROOT DEFECTIVE 3 isoform X2 [Cucumis sativus][more]
XP_011653614.12.20e-3286.96protein SODIUM POTASSIUM ROOT DEFECTIVE 3 isoform X1 [Cucumis sativus] >KGN54216... [more]
XP_008447442.15.27e-2174.60PREDICTED: uncharacterized protein LOC103489885 [Cucumis melo] >KAA0047336.1 HMA... [more]
KAG6603681.17.61e-1865.15Protein SODIUM POTASSIUM ROOT DEFECTIVE 3, partial [Cucurbita argyrosperma subsp... [more]
KAG7033858.18.10e-1865.15Protein SODIUM POTASSIUM ROOT DEFECTIVE 3, partial [Cucurbita argyrosperma subsp... [more]
BLAST of Cucsat.G13821 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L2391.07e-3286.96HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G293260 PE=4 SV... [more]
A0A5A7TZF02.55e-2174.60HMA domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3BIC72.55e-2174.60uncharacterized protein LOC103489885 OS=Cucumis melo OX=3656 GN=LOC103489885 PE=... [more]
A0A6J1ILS74.29e-1865.15protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GEB02.85e-1763.64protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like OS=Cucurbita moschata OX=3662 GN=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 52..101
e-value: 3.8E-12
score: 46.4
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 48..105
score: 10.675345
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 51..104
e-value: 2.32159E-12
score: 56.0749
NoneNo IPR availableGENE3D3.30.70.100coord: 45..109
e-value: 1.4E-15
score: 59.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..136
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..48
NoneNo IPR availablePANTHERPTHR46119:SF15PROTEIN SODIUM POTASSIUM ROOT DEFECTIVE 2coord: 23..124
IPR044526Protein SODIUM POTASSIUM ROOT DEFECTIVE 1/2/3PANTHERPTHR46119OS08G0405700 PROTEINcoord: 23..124
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 46..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G13821.T2Cucsat.G13821.T2mRNA
Cucsat.G13821.T1Cucsat.G13821.T1mRNA
Cucsat.G13821.T3Cucsat.G13821.T3mRNA
Cucsat.G13821.T6Cucsat.G13821.T6mRNA
Cucsat.G13821.T4Cucsat.G13821.T4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding