Cucsat.G13729 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G13729
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Glutathione S-transferase family protein
Location	ctg184: 1707447 .. 1713397 (-)
RNA-Seq Expression	Cucsat.G13729
Synteny	Cucsat.G13729

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAATAAAGACTCAGCTTTTGTGTATTATCATTCTTGTTATTAAAAACAATCAGACAGAGATTAGCAATATCTATGGCGATGGGGGAAGAAGTGGAATTGTTTGGTGGATGGATGAGTCCATTTAGCCGGAGAGTGGAGTTGGGTTTGAAACTCAAAGGCATTGATTATAAATATCATGAAGAAGATTTGAAGAACAAAAGTGATTTCCTTTTGACTTACAACCCAATTTACAAGAAAGTCCCTGTTTTTCTTCACAACGGAAACCCCATCTCAGAGTCACTTATAATTCTTGAGTACATCGATCAAGTTTGGAACTCACTTTATCCTTTCTTTCCTCAACAAAATCCCTACGAAACAGCTCAAGCAAGATTCTGGGCTAATTATATAGATGACAAGGTATGTAAGTAAATTTGTTTATTTTATTGATCACTGCTTACTTTAACGGTATCAATGTGTTCATAATATATATAATTTCAATTCATGATATAGGTTTTGGCTGCTACACTGAAGGCTGCAAAAAGCAGCAAAAGAGAAGAGAGAGAGAAGGGTTTGGAAGAAACAGAGGAGACATTAAAACCACTTGAGAAAGAGCTTCAAAACAAGAAGTTCTTTGGAGGAAATAAAATTGGAATTGTGGACATTGTTGGAACTGTAATTGCATATTGGATCCCAGCCATTGAAGAAGCTTTTGGATTTGAGCTGTTAACAACAAAAAAGTTTCCAAAGTTAACCAAATGGAGTGAAGAGATTGTAAACAACAGTGTGGTCAAACAGGTTTTGCCTCCAAAATCTAAACTTGTGGCCTACTTCGAGTTGCAAGTTGTTTCAATAACCGACTAGACAATATGTTGCTTTCTTTTGTATCTTCAATAGACCACCTGCGTTATGTAACAAAATAAATCTCTAACCTTTTGGTAAGGTCGAGAATGTTCTCTAATTACCTATGTATTTTGCTCCTTCCCAGCAATCATTTTCTTTATGGCTATTTTCAAAT

Coding sequence (CDS)

ATGGCGATGGGGGAAGAAGTGGAATTGTTTGGTGGATGGATGAGTCCATTTAGCCGGAGAGTGGAGTTGGGTTTGAAACTCAAAGGCATTGATTATAAATATCATGAAGAAGATTTGAAGAACAAAAGTGATTTCCTTTTGACTTACAACCCAATTTACAAGAAAGTCCCTGTTTTTCTTCACAACGGAAACCCCATCTCAGAGTCACTTATAATTCTTGAGTACATCGATCAAGTTTGGAACTCACTTTATCCTTTCTTTCCTCAACAAAATCCCTACGAAACAGCTCAAGCAAGATTCTGGGCTAATTATATAGATGACAAGGTTTTGGCTGCTACACTGAAGGCTGCAAAAAGCAGCAAAAGAGAAGAGAGAGAGAAGGGTTTGGAAGAAACAGAGGAGACATTAAAACCACTTGAGAAAGAGCTTCAAAACAAGAAGTTCTTTGGAGGAAATAAAATTGGAATTGTGGACATTGTTGGAACTGTAATTGCATATTGGATCCCAGCCATTGAAGAAGCTTTTGGATTTGAGCTGTTAACAACAAAAAAGTTTCCAAAGTTAACCAAATGGAGTGAAGAGATTGTAAACAACAGTGTGGTCAAACAGGTTTTGCCTCCAAAATCTAAACTTGTGGCCTACTTCGAGTTGCAAGTTGTTTCAATAACCGACTAG

Protein sequence

MAMGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHNGNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWANYIDDKVLAATLKAAKSSKREEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTTKKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSITD

Homology

[Close]

BLAST of Cucsat.G13729 vs. ExPASy Swiss-Prot
Match: P32110 (Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 7.1e-60
Identity = 114/219 (52.05%), Postives = 159/219 (72.60%), Query Frame = 0


Query: 3   EEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHNGN 62
           E+V+L G   SPF  RV++ LKLKG++YK+ EE+L NKSD LL YNP++KKVPVF+HN  
Sbjct: 6   EDVKLLGIVGSPFVCRVQIALKLKGVEYKFLEENLGNKSDLLLKYNPVHKKVPVFVHNEQ 65

Query: 63  PISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKREE 122
           PI+ESL+I+EYID+ W +  P  P  +PY+ A ARFW+K+IDDK++ A  K+  +   +E
Sbjct: 66  PIAESLVIVEYIDETWKN-NPILP-SDPYQRALARFWSKFIDDKIVGAVSKSVFTVDEKE 125

Query: 123 REKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTTKK 182
           REK +EET E L+ LE EL++KKFFGG + G+VDI    IA+WIP  +E  G +L T++K
Sbjct: 126 REKNVEETYEALQFLENELKDKKFFGGEEFGLVDIAAVFIAFWIPIFQEIAGLQLFTSEK 185

Query: 183 FPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSIT 222
           FP L KWS+E +N+  V +VLPP+  L AYF+ +  S++
Sbjct: 186 FPILYKWSQEFLNHPFVHEVLPPRDPLFAYFKARYESLS 222

[Close]

BLAST of Cucsat.G13729 vs. ExPASy Swiss-Prot
Match: Q9SR36 (Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.6e-59
Identity = 118/220 (53.64%), Postives = 153/220 (69.55%), Query Frame = 0


Query: 3   EEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDL-KNKSDFLLTYNPIYKKVPVFLHNG 62
           E V+L G W SPFS+RVE+ LKLKGI Y+Y EED+  N+S  LL YNPI+KKVPV +HNG
Sbjct: 5   EHVKLLGLWGSPFSKRVEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNG 64

Query: 63  NPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKRE 122
             I+ESL+I+EYI+  W + +   P Q+PYE A ARFWAKY+D+KV+ A  KA    +  
Sbjct: 65  RSIAESLVIVEYIEDTWKTTHTILP-QDPYERAMARFWAKYVDEKVMLAVKKACWGPE-S 124

Query: 123 EREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTTK 182
           EREK ++E  E LK LEKEL +K FFGG  IG VDI    I YW+   +EA G  ++T +
Sbjct: 125 EREKEVKEAYEGLKCLEKELGDKLFFGGETIGFVDIAADFIGYWLGIFQEASGVTIMTAE 184

Query: 183 KFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSIT 222
           +FPKL +WSE+ V N+ +K+VLPPK KLVA  +    S+T
Sbjct: 185 EFPKLQRWSEDFVGNNFIKEVLPPKEKLVAVLKAMFGSVT 222

[Close]

BLAST of Cucsat.G13729 vs. ExPASy Swiss-Prot
Match: Q03663 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 3.4e-54
Identity = 106/217 (48.85%), Postives = 145/217 (66.82%), Query Frame = 0


Query: 4   EVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHNGNP 63
           EV+L G W SPFS RVE  LK+KG+ Y+Y EED  NKS  LL  NP+YKKVPV +HNG P
Sbjct: 3   EVKLLGFWYSPFSHRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNPVYKKVPVLIHNGKP 62

Query: 64  ISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKREER 123
           I ES+IILEYID+ +    P    ++PY+ A ARFWAK++DDKV A      +  K EE+
Sbjct: 63  IVESMIILEYIDETFEG--PSILPKDPYDRALARFWAKFLDDKVAAVVNTFFR--KGEEQ 122

Query: 124 EKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTTKKF 183
           EKG EE  E LK L+ EL++KKFF G+K G  DI   ++ +W+   EE +G  L+ ++KF
Sbjct: 123 EKGKEEVYEMLKVLDNELKDKKFFAGDKFGFADIAANLVGFWLGVFEEGYGDVLVKSEKF 182

Query: 184 PKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSI 221
           P  +KW +E +N S V + LPP+ +L+A+F  +  ++
Sbjct: 183 PNFSKWRDEYINCSQVNESLPPRDELLAFFRARFQAV 215

[Close]

BLAST of Cucsat.G13729 vs. ExPASy Swiss-Prot
Match: Q03662 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-52
Identity = 106/210 (50.48%), Postives = 142/210 (67.62%), Query Frame = 0


Query: 4   EVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHNGNP 63
           EV+L G W SPFSRRVE  LK+KG+ Y+Y EED  NKS  LL  NPI+KKVPV +HNG  
Sbjct: 3   EVKLLGFWYSPFSRRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNPIHKKVPVLIHNGKR 62

Query: 64  ISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKREER 123
           I ES++ILEYID+ +    P    ++PY+ A ARFWAK++DDKV A      +  K EE+
Sbjct: 63  IVESMVILEYIDETFEG--PSILPKDPYDRALARFWAKFLDDKVPAVVKTFLR--KGEEQ 122

Query: 124 EKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTTKKF 183
           EK  EE  E LK L+ EL++KKFF G+K G  DI   ++A+W+   EEA G  L+T++KF
Sbjct: 123 EKDKEEVCEMLKVLDNELKDKKFFVGDKFGFADIAANLVAFWLGVFEEASGVVLVTSEKF 182

Query: 184 PKLTKWSEEIVNNSVVKQVLPPKSKLVAYF 214
           P   KW  E +N S +K+ LPP+ +L+A++
Sbjct: 183 PNFCKWRGEYINCSQIKESLPPRDELLAFY 208

[Close]

BLAST of Cucsat.G13729 vs. ExPASy Swiss-Prot
Match: Q03664 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 4.2e-52
Identity = 102/217 (47.00%), Postives = 145/217 (66.82%), Query Frame = 0


Query: 4   EVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHNGNP 63
           EV+L G W SPF+ RVE  LKLKG+ Y+Y EED  NKS  LL  NP++KKVPV +HNG P
Sbjct: 3   EVKLLGFWYSPFTHRVEWALKLKGVKYEYIEEDRDNKSSLLLQSNPVHKKVPVLIHNGKP 62

Query: 64  ISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKREER 123
           I ES++ILEYID+ +    P    ++PY+ A ARFW+K++ DKV A      +  K EE+
Sbjct: 63  IVESMVILEYIDETFEG--PSILPKDPYDRALARFWSKFLGDKVAAVVNTFFR--KGEEQ 122

Query: 124 EKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTTKKF 183
           EKG EE  E LK L+ EL++KKFF G+K G  DI   ++ +W+   EE +G  L+T++KF
Sbjct: 123 EKGKEEVYEMLKVLDNELKDKKFFVGDKFGFADIAANLVGFWLGVFEEGYGVVLVTSEKF 182

Query: 184 PKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSI 221
           P  ++W +E +N S VK+ LP + +L+A+F  +  ++
Sbjct: 183 PNFSRWRDEYINCSQVKESLPSRDELLAFFRARFQAV 215

[Close]

BLAST of Cucsat.G13729 vs. NCBI nr
Match: KAE8649558.1 (hypothetical protein Csa_018097 [Cucumis sativus])

HSP 1 Score: 444 bits (1141), Expect = 4.12e-157
Identity = 221/222 (99.55%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN
Sbjct: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR
Sbjct: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT
Sbjct: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSITD 222
           KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSIT+
Sbjct: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSITN 222

[Close]

BLAST of Cucsat.G13729 vs. NCBI nr
Match: XP_031739688.1 (probable glutathione S-transferase [Cucumis sativus])

HSP 1 Score: 442 bits (1136), Expect = 2.56e-156
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN
Sbjct: 3   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 62

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWA YIDDKVLAATLKAAKSSKR
Sbjct: 63  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWANYIDDKVLAATLKAAKSSKR 122

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT
Sbjct: 123 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 182

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSITD 222
           KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSIT+
Sbjct: 183 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSITN 224

[Close]

BLAST of Cucsat.G13729 vs. NCBI nr
Match: XP_004147912.2 (probable glutathione S-transferase [Cucumis sativus] >KGN54320.1 hypothetical protein Csa_018173 [Cucumis sativus])

HSP 1 Score: 422 bits (1086), Expect = 1.00e-148
Identity = 209/214 (97.66%), Postives = 210/214 (98.13%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           M EEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN
Sbjct: 3   MAEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 62

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWA YIDDKVLAATLKAAKSSKR
Sbjct: 63  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWANYIDDKVLAATLKAAKSSKR 122

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT
Sbjct: 123 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 182

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFE 214
           KKFPKLTKWSEEIVNNSVVKQVLPPKS LVAY +
Sbjct: 183 KKFPKLTKWSEEIVNNSVVKQVLPPKSNLVAYLQ 216

[Close]

BLAST of Cucsat.G13729 vs. NCBI nr
Match: XP_004147931.3 (glutathione S-transferase U8 [Cucumis sativus])

HSP 1 Score: 414 bits (1063), Expect = 2.99e-145
Identity = 204/215 (94.88%), Postives = 209/215 (97.21%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN
Sbjct: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR
Sbjct: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EE+EKGLEETEETLKPLEKELQNKKFFGGN+IG VDIVGTVIAYWIPAIEE FG ELLT+
Sbjct: 121 EEKEKGLEETEETLKPLEKELQNKKFFGGNQIGFVDIVGTVIAYWIPAIEEGFGSELLTS 180

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFEL 215
           KKFPKLTKWSEEIV NSVVKQVLPPKS LVAY ++
Sbjct: 181 KKFPKLTKWSEEIVKNSVVKQVLPPKSVLVAYLQV 215

[Close]

BLAST of Cucsat.G13729 vs. NCBI nr
Match: XP_008448766.1 (PREDICTED: probable glutathione S-transferase [Cucumis melo] >KAA0045101.1 putative glutathione S-transferase [Cucumis melo var. makuwa] >TYJ96227.1 putative glutathione S-transferase [Cucumis melo var. makuwa])

HSP 1 Score: 406 bits (1044), Expect = 2.53e-142
Identity = 199/215 (92.56%), Postives = 208/215 (96.74%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           MGEEVELFGGWMSPFSRRVELGLKLKGI+YKY+EEDLKNKSDFLL YNPIYKKVPVFLHN
Sbjct: 3   MGEEVELFGGWMSPFSRRVELGLKLKGINYKYNEEDLKNKSDFLLNYNPIYKKVPVFLHN 62

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYID+VWNS+YPFFPQQNPYE AQARFWAKYIDDKV+AA LKAAKSSKR
Sbjct: 63  GNPISESLIILEYIDEVWNSVYPFFPQQNPYERAQARFWAKYIDDKVVAAILKAAKSSKR 122

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EEREKGLEETEETL+PLEKELQNKKFFGGNKIG VDIVGTVIAYWIPAIEEAFGFE+LTT
Sbjct: 123 EEREKGLEETEETLEPLEKELQNKKFFGGNKIGFVDIVGTVIAYWIPAIEEAFGFEVLTT 182

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFEL 215
           KKFP LTKWSEEIVN SVVKQVLPPKS LVAYF++
Sbjct: 183 KKFPNLTKWSEEIVNQSVVKQVLPPKSNLVAYFQV 217

[Close]

BLAST of Cucsat.G13729 vs. ExPASy TrEMBL
Match: A0A0A0KXE0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G303680 PE=3 SV=1)

HSP 1 Score: 422 bits (1086), Expect = 4.84e-149
Identity = 209/214 (97.66%), Postives = 210/214 (98.13%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           M EEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN
Sbjct: 3   MAEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 62

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWA YIDDKVLAATLKAAKSSKR
Sbjct: 63  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWANYIDDKVLAATLKAAKSSKR 122

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT
Sbjct: 123 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 182

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFE 214
           KKFPKLTKWSEEIVNNSVVKQVLPPKS LVAY +
Sbjct: 183 KKFPKLTKWSEEIVNNSVVKQVLPPKSNLVAYLQ 216

[Close]

BLAST of Cucsat.G13729 vs. ExPASy TrEMBL
Match: A0A0A0KXE8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G304760 PE=3 SV=1)

HSP 1 Score: 417 bits (1071), Expect = 8.72e-147
Identity = 206/215 (95.81%), Postives = 210/215 (97.67%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN
Sbjct: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR
Sbjct: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EE+EKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEE FG ELLT+
Sbjct: 121 EEKEKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEGFGSELLTS 180

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFEL 215
           KKFPKLTKWSEEIV NSVVKQVLPPKS LVAY ++
Sbjct: 181 KKFPKLTKWSEEIVKNSVVKQVLPPKSVLVAYLQV 215

[Close]

BLAST of Cucsat.G13729 vs. ExPASy TrEMBL
Match: A0A5D3BAT9 (Putative glutathione S-transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold78209G00150 PE=3 SV=1)

HSP 1 Score: 406 bits (1044), Expect = 1.22e-142
Identity = 199/215 (92.56%), Postives = 208/215 (96.74%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           MGEEVELFGGWMSPFSRRVELGLKLKGI+YKY+EEDLKNKSDFLL YNPIYKKVPVFLHN
Sbjct: 3   MGEEVELFGGWMSPFSRRVELGLKLKGINYKYNEEDLKNKSDFLLNYNPIYKKVPVFLHN 62

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYID+VWNS+YPFFPQQNPYE AQARFWAKYIDDKV+AA LKAAKSSKR
Sbjct: 63  GNPISESLIILEYIDEVWNSVYPFFPQQNPYERAQARFWAKYIDDKVVAAILKAAKSSKR 122

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EEREKGLEETEETL+PLEKELQNKKFFGGNKIG VDIVGTVIAYWIPAIEEAFGFE+LTT
Sbjct: 123 EEREKGLEETEETLEPLEKELQNKKFFGGNKIGFVDIVGTVIAYWIPAIEEAFGFEVLTT 182

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFEL 215
           KKFP LTKWSEEIVN SVVKQVLPPKS LVAYF++
Sbjct: 183 KKFPNLTKWSEEIVNQSVVKQVLPPKSNLVAYFQV 217

[Close]

BLAST of Cucsat.G13729 vs. ExPASy TrEMBL
Match: A0A1S3BL35 (probable glutathione S-transferase OS=Cucumis melo OX=3656 GN=LOC103490830 PE=3 SV=1)

HSP 1 Score: 406 bits (1044), Expect = 1.22e-142
Identity = 199/215 (92.56%), Postives = 208/215 (96.74%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           MGEEVELFGGWMSPFSRRVELGLKLKGI+YKY+EEDLKNKSDFLL YNPIYKKVPVFLHN
Sbjct: 3   MGEEVELFGGWMSPFSRRVELGLKLKGINYKYNEEDLKNKSDFLLNYNPIYKKVPVFLHN 62

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYID+VWNS+YPFFPQQNPYE AQARFWAKYIDDKV+AA LKAAKSSKR
Sbjct: 63  GNPISESLIILEYIDEVWNSVYPFFPQQNPYERAQARFWAKYIDDKVVAAILKAAKSSKR 122

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EEREKGLEETEETL+PLEKELQNKKFFGGNKIG VDIVGTVIAYWIPAIEEAFGFE+LTT
Sbjct: 123 EEREKGLEETEETLEPLEKELQNKKFFGGNKIGFVDIVGTVIAYWIPAIEEAFGFEVLTT 182

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFEL 215
           KKFP LTKWSEEIVN SVVKQVLPPKS LVAYF++
Sbjct: 183 KKFPNLTKWSEEIVNQSVVKQVLPPKSNLVAYFQV 217

[Close]

BLAST of Cucsat.G13729 vs. ExPASy TrEMBL
Match: A0A1S3BKT3 (probable glutathione S-transferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490752 PE=3 SV=1)

HSP 1 Score: 399 bits (1024), Expect = 1.37e-139
Identity = 200/222 (90.09%), Postives = 209/222 (94.14%), Query Frame = 0


Query: 1   MGEEVELFGGWMSPFSRRVELGLKLKGIDYKYHEEDLKNKSDFLLTYNPIYKKVPVFLHN 60
           MGEEVEL GGWMSPFSRRVELGLKLKGI+YKY+EEDLKNKSDFLL YNPIYKKVPVFLHN
Sbjct: 3   MGEEVELLGGWMSPFSRRVELGLKLKGINYKYNEEDLKNKSDFLLNYNPIYKKVPVFLHN 62

Query: 61  GNPISESLIILEYIDQVWNSLYPFFPQQNPYETAQARFWAKYIDDKVLAATLKAAKSSKR 120
           GNPISESLIILEYID+VWNS+YPFFPQQNPYE AQARFWAKYIDDKV+AA LKAAKSSKR
Sbjct: 63  GNPISESLIILEYIDEVWNSVYPFFPQQNPYERAQARFWAKYIDDKVVAAILKAAKSSKR 122

Query: 121 EEREKGLEETEETLKPLEKELQNKKFFGGNKIGIVDIVGTVIAYWIPAIEEAFGFELLTT 180
           EEREKGLEETEETL+PLEKELQNKKFFGGNKIG VDIVGTVIAYWIPA+EEAFGFE+LTT
Sbjct: 123 EEREKGLEETEETLEPLEKELQNKKFFGGNKIGFVDIVGTVIAYWIPAVEEAFGFEVLTT 182

Query: 181 KKFPKLTKWSEEIVNNSVVKQVLPPKSKLVAYFELQVVSITD 222
           KKFP LTKWSEEIVN SVVKQVLP KS LVAY  LQVV  T+
Sbjct: 183 KKFPNLTKWSEEIVNQSVVKQVLPLKSNLVAY--LQVVLTTN 222

The following BLAST results are available for this feature:

BLAST of Cucsat.G13729 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P32110	7.1e-60	52.05	Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1	[more]
Q9SR36	4.6e-59	53.64	Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1	[more]
Q03663	3.4e-54	48.85	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q03662	1.4e-52	50.48	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q03664	4.2e-52	47.00	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

BLAST of Cucsat.G13729 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649558.1	4.12e-157	99.55	hypothetical protein Csa_018097 [Cucumis sativus]	[more]
XP_031739688.1	2.56e-156	99.10	probable glutathione S-transferase [Cucumis sativus]	[more]
XP_004147912.2	1.00e-148	97.66	probable glutathione S-transferase [Cucumis sativus] >KGN54320.1 hypothetical pr...	[more]
XP_004147931.3	2.99e-145	94.88	glutathione S-transferase U8 [Cucumis sativus]	[more]
XP_008448766.1	2.53e-142	92.56	PREDICTED: probable glutathione S-transferase [Cucumis melo] >KAA0045101.1 putat...	[more]

BLAST of Cucsat.G13729 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXE0	4.84e-149	97.66	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G303680 PE=3 SV=1	[more]
A0A0A0KXE8	8.72e-147	95.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G304760 PE=3 SV=1	[more]
A0A5D3BAT9	1.22e-142	92.56	Putative glutathione S-transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BL35	1.22e-142	92.56	probable glutathione S-transferase OS=Cucumis melo OX=3656 GN=LOC103490830 PE=3 ...	[more]
A0A1S3BKT3	1.37e-139	90.09	probable glutathione S-transferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004358	Signal transduction histidine kinase-related protein, C-terminal	PRINTS	PR00344	BCTRLSENSOR	coord: 347..360 score: 28.11 coord: 277..291 score: 40.34 coord: 323..341 score: 35.59
IPR003594	Histidine kinase/HSP90-like ATPase	SMART	SM00387	HKATPase_4	coord: 232..363 e-value: 3.3E-14 score: 63.2
IPR003594	Histidine kinase/HSP90-like ATPase	PFAM	PF02518	HATPase_c	coord: 234..360 e-value: 6.9E-15 score: 55.5
IPR036784	Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamily	GENE3D	1.20.140.20		coord: 5..182 e-value: 9.8E-63 score: 213.2
IPR036784	Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamily	SUPERFAMILY	69012	alpha-ketoacid dehydrogenase kinase, N-terminal domain	coord: 17..180
IPR018955	Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase, N-terminal	PFAM	PF10436	BCDHK_Adom3	coord: 28..190 e-value: 2.6E-56 score: 190.0
IPR036890	Histidine kinase/HSP90-like ATPase superfamily	GENE3D	3.30.565.10		coord: 192..369 e-value: 2.1E-52 score: 178.9
IPR036890	Histidine kinase/HSP90-like ATPase superfamily	SUPERFAMILY	55874	ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase	coord: 190..359
IPR039028	PDK/BCKDK protein kinase	PANTHER	PTHR11947	PYRUVATE DEHYDROGENASE KINASE	coord: 10..370
None	No IPR available	PANTHER	PTHR11947:SF42	BNAA05G30880D PROTEIN	coord: 10..370
None	No IPR available	CDD	cd16929	HATPase_PDK-like	coord: 194..360 e-value: 9.3121E-80 score: 239.935
IPR005467	Histidine kinase domain	PROSITE	PS50109	HIS_KIN	coord: 234..363 score: 18.049038

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G13729.T8	Cucsat.G13729.T8	mRNA
Cucsat.G13729.T13	Cucsat.G13729.T13	mRNA
Cucsat.G13729.T5	Cucsat.G13729.T5	mRNA
Cucsat.G13729.T14	Cucsat.G13729.T14	mRNA
Cucsat.G13729.T7	Cucsat.G13729.T7	mRNA
Cucsat.G13729.T1	Cucsat.G13729.T1	mRNA
Cucsat.G13729.T2	Cucsat.G13729.T2	mRNA
Cucsat.G13729.T11	Cucsat.G13729.T11	mRNA
Cucsat.G13729.T10	Cucsat.G13729.T10	mRNA
Cucsat.G13729.T9	Cucsat.G13729.T9	mRNA
Cucsat.G13729.T4	Cucsat.G13729.T4	mRNA
Cucsat.G13729.T3	Cucsat.G13729.T3	mRNA
Cucsat.G13729.T12	Cucsat.G13729.T12	mRNA
Cucsat.G13729.T6	Cucsat.G13729.T6	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006749	glutathione metabolic process
biological_process	GO:0046777	protein autophosphorylation
biological_process	GO:0010906	regulation of glucose metabolic process
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004364	glutathione transferase activity
molecular_function	GO:0009927	histidine phosphotransfer kinase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0004740	pyruvate dehydrogenase (acetyl-transferring) kinase activity
molecular_function	GO:0008194	UDP-glycosyltransferase activity
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0016772	transferase activity, transferring phosphorus-containing groups