Cucsat.G13388 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G13388
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	cytochrome P450 CYP82D47-like
Location	ctg1838: 5850025 .. 5852754 (+)
RNA-Seq Expression	Cucsat.G13388
Synteny	Cucsat.G13388

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AACATTCTTTTGAAAATACCCTTTTTCCAATTTGTTTTAAAAACAACCCCTTTTTTTTTTTTCAATTGTCCCATATAATGGTCCATATCATTGATTTAAAATTAAATTTCAAAACCATTTTTTCTTCTCCCTGACGAACTAATTAATGCTATCTTAGATGAGGGATAACCAATTTCGCAACTGCTAATATCTTCACCACTATCATTGGGGCTTCCCACACGCACCGCCATGGCAGATCACATAATTTCTCCATATGGTATCAACATCTCCATAGTTAGATTACTCTTCCTCTTCCCTATTCTCATCTTCTCATATTATATTATTCTAATTCTCAAGAACAAAGCTACAACATTACCTCATAAACTCCGATCGCAACCCCCTGAAGTAGCCGGCGGATGGCCGATCATTGGCCATCTACTCCTCCTCCTCAGAACTCACTCTCTGCTCCCCCACGAAACCTTTGGAGCATTGGCCGACAAATATGGCCCGATATTCCTAATCCGACTCGGCGTCCATCCAACTCTTGTCATAAACAATTGGGAAATCGCCAAAGAATGCTACACAACTCTTGATTCAATCGTCTCATCCCGACCCAAGACTTTAATTCAAAAGGAATTAAGCTACAACTTCGCCGGGTTTGGGTTCAGGCCAAAGTACGATGCTTTTTACCGAAAACATGCGTAAAATGGCAGTCTCCGAAGTGCTTTCGAATAGCCGTCTGGAAATACAGAGAGATATGAGAGTTTATGAGGGGTGAACAGAGGAGTGAAGGAGATATACAACTCATGGACAGAGTACAGAAATGAGGATTTAATTGCTGTTGATTTGGATGAATGGATTGGGAATATTAATTTGAATGTGATTTTGAGGATGGTTTGTGGGAAGCGGATGGCTGAGGGGTCGGAGATGGAACGATGTCGGAAAGCGATGAGGGGTTTTTTTGAATTGGCAGGTCAGGTAACGGTGGGAGATGCGATTCCATTTCTAAAATTTTTGGATTTGGGTGGTTATTTGAAGACGACTAAGGAAGTTTTTAAGGAATTGGATTGTATCATGGAGGAATGGCTTGAAGAACATCGTCAGAAGAAGAGAGACGCGGGCGCCAGTGCTGGTGCCGCGGAGAAGGGTCTCATGGGTGTGATGCCTTTGCTTCTCAAAGGGATGGAGCTTGATGGGTATGATGCTGATACTGTCATTAAAGCCACTTGCCTGGTATGCTATTAACACTTATTTATTCATGAGCTATCACTATATATACATTCGAAAAAATTACAAAAACACAGTTTAGGTTTTCCTTTCAAAAGTGAAACACTTTTAAAGACTTATGATTTTGTTGTGATCCTCTCCGAGATTTCATCCCGGCTGGGATGCATTCTACTATGTTCATAAAAACTTGGGACAAATTTCTGATACACAAAATTACATGAAATCTCGAGGATGATCTAAATCGATTGAGATTGCTCGAGAAAAACAAAAGCCTAAGTTTTTAAAAAGTGTCCTACTAATTTTGAAATGCAAAACTTAAATAGTATTGTTTTTGTCATTTTGATATATTTTGTAAATATTTTTGTTCATTTAATATTGAAAAATCTCACGAATAAGATCTGTATGATGTTTATTTTAGAATTATTATTCTCATAGTAATCAACCAGCTGATAATATAAATTTGAGATATTGTGTAACACAACTTCTCTATCATACATTTAGCCATAAAATCTGTCAATAACCTTGGCAACTGTATTTAAAGAATTTCTTTTCTTTTATGGTAGACACTTATTTCTGGAGGAACCGATACAATGACAGTAACTATAACATGGGCAATCTCATTGTTACTTAACAACCAAGATGCATTAAGAAGAGTACAAGAGGAGTTAGACATCCATGTTGGAAACAAAAGACTAGTGAATGAATCAGACATAAACAAGCTAGTGTATCTTCAAGCTGTAATCAAAGAGACATTAAGGTTGTACCCAGCAGGGCCATTGTCTGGGGCTCGAGAGGTAACCGAGGATTGCACAATTGGAGGCTACAATGTGGCGTCAGGCACACACCTTATCACAAACATTTGGAAGATTCAGAGGGACCCTCGAGTTTGGACAGAGCCTTCAAAGTTCAAACCGGAGAGGTTTCTTAGTAGCCATAACTATGACTACATGGATGTGAAGGGCCAACATTTTGAATTGTCTCCATTTGGTTATGGAAGAAGGGTCTGCCCTGGGTTAGGTATTAGCCTTATGATGACGCCACTGGTGTTGGCTAGTTTGATTCATTCGTTTGATGTTACAACTTATGATGATGAACCAGTGGACATGGCTGCAAACCTTGGGCTCACCATGCGCAGAGTCAAACCTCTTCATGTTCTCGTTAAGCCGCGTCTCCTAGCTACTGCTTATGCATAATCTCAGGCAACTTTAACTTGTACTGCTTTTGTAGTCTCTATGCATCACCTCCAATTCAACCACGAAGAAAAACGTTTTAAAGTATATTTTAAAAGAAAATTTTAGCACAAGTCAACCTATAATCGATCAATAGCTATATGCCAACTTCGAATATAGTATTATGAGATGAATAAACTTGTAGAGTGGAATAAAAGTAAATGTAATAATTATAGTTTATGCTAAAGTTTTTGATGGTAGGATGGTTTGTCTTAAACAAAATTGATAATAAAAATCAATGTTATTGCAGCCTCAATGTTAGCAAATTTATAAACAAACTCTACAATTTTTAAAT

Coding sequence (CDS)

ATGGTTTGTGGGAAGCGGATGGCTGAGGGGTCGGAGATGGAACGATGTCGGAAAGCGATGAGGGGTTTTTTTGAATTGGCAGGTCAGGTAACGGTGGGAGATGCGATTCCATTTCTAAAATTTTTGGATTTGGGTGGTTATTTGAAGACGACTAAGGAAGTTTTTAAGGAATTGGATTGTATCATGGAGGAATGGCTTGAAGAACATCGTCAGAAGAAGAGAGACGCGGGCGCCAGTGCTGGTGCCGCGGAGAAGGGTCTCATGGGTGTGATGCCTTTGCTTCTCAAAGGGATGGAGCTTGATGGGTATGATGCTGATACTGTCATTAAAGCCACTTGCCTGACACTTATTTCTGGAGGAACCGATACAATGACAGTAACTATAACATGGGCAATCTCATTGTTACTTAACAACCAAGATGCATTAAGAAGAGTACAAGAGGAGTTAGACATCCATGTTGGAAACAAAAGACTAGTGAATGAATCAGACATAAACAAGCTAGTGTATCTTCAAGCTGTAATCAAAGAGACATTAAGGTTGTACCCAGCAGGGCCATTGTCTGGGGCTCGAGAGGTAACCGAGGATTGCACAATTGGAGGCTACAATGTGGCGTCAGGCACACACCTTATCACAAACATTTGGAAGATTCAGAGGGACCCTCGAGTTTGGACAGAGCCTTCAAAGTTCAAACCGGAGAGGTTTCTTAGTAGCCATAACTATGACTACATGGATGTGAAGGGCCAACATTTTGAATTGTCTCCATTTGGTTATGGAAGAAGGGTCTGCCCTGGGTTAGGTATTAGCCTTATGATGACGCCACTGGTGTTGGCTAGTTTGATTCATTCGTTTGATGTTACAACTTATGATGATGAACCAGTGGACATGGCTGCAAACCTTGGGCTCACCATGCGCAGAGTCAAACCTCTTCATGTTCTCGTTAAGCCGCGTCTCCTAGCTACTGCTTATGCATAA

Protein sequence

MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLTLISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLGLTMRRVKPLHVLVKPRLLATAYA

Homology

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BLAST of Cucsat.G13388 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.3e-88
Identity = 173/326 (53.07%), Postives = 212/326 (65.03%), Query Frame = 0


Query: 1   MVCGKRM----AEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFK 60
           MV GKR     A   + E+C KA+  F  LAG  TVGDAIP+L++LD GGY K  KE  K
Sbjct: 202 MVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKAMKETAK 261

Query: 61  ELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLTL 120
           ELD ++ EWLEEHRQK+       GA +   M VM   L G  +DG DADT+IK+T LT+
Sbjct: 262 ELDVMISEWLEEHRQKRALGEGVDGAQD--FMNVMLSSLDGKTIDGIDADTLIKSTVLTI 321

Query: 121 ISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKE 180
           I  GT+    TI WA+ L+L N   L   + ELDI VG  R + ESDI+ LVYLQAV+KE
Sbjct: 322 IQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKDRCICESDISNLVYLQAVVKE 381

Query: 181 TLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLS 240
           TLRLY  GPLS  RE  EDCT+GGY+V  GT LITNIWKI  DP VW++P +FKP+RFL+
Sbjct: 382 TLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNVWSDPFEFKPDRFLT 441

Query: 241 SHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMA 300
           +H    +DVKG HF+L PFG GRRVCPG+   L    L LAS +HSF++     EP+DM 
Sbjct: 442 THK--DIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFEILNPSTEPLDMT 501

Query: 301 ANLGLTMRRVKPLHVLVKPRLLATAY 323
              G+T  +  PL VLVKP L  + Y
Sbjct: 502 EAFGVTNTKATPLEVLVKPCLSPSCY 522

[Close]

BLAST of Cucsat.G13388 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 9.0e-88
Identity = 169/328 (51.52%), Postives = 219/328 (66.77%), Query Frame = 0


Query: 1   MVCGKRMAEG---------SEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTT 60
           MV GKR   G          E  +C+KA+  FF L G  TV DA P L F DL G+ K  
Sbjct: 194 MVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEM 253

Query: 61  KEVFKELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELD--GYDADTVI 120
           K+   ELD I+E W+E HRQ+++ +G      +   + VM  L +  +L    YDA+T I
Sbjct: 254 KQTGSELDVILERWIENHRQQRKFSGTKEN--DSDFIDVMMSLAEQGKLSHLQYDANTSI 313

Query: 121 KATCLTLISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVY 180
           K+TCL LI GG+DT   T+TWAISLLLNN++ L++ Q+E+DIHVG  R V +SDI  LVY
Sbjct: 314 KSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVY 373

Query: 181 LQAVIKETLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKF 240
           LQA+IKETLRLYPAGPL G RE  EDCT+ GY V  GT LI N+WKIQRDP+V+ EP++F
Sbjct: 374 LQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEF 433

Query: 241 KPERFLSSHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYD 300
           +PERF++    ++ DV+GQ+FEL PFG GRR CPG  +++ +  L LA  +HSFDV T  
Sbjct: 434 RPERFITGEAKEF-DVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVM 493

Query: 301 DEPVDMAANLGLTMRRVKPLHVLVKPRL 318
           D PVDM+ N GLT+ +  PL VL+ PR+
Sbjct: 494 DMPVDMSENPGLTIPKATPLEVLISPRI 518

[Close]

BLAST of Cucsat.G13388 vs. ExPASy Swiss-Prot
Match: O49396 (Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3)

HSP 1 Score: 322.4 bits (825), Expect = 5.8e-87
Identity = 170/328 (51.83%), Postives = 219/328 (66.77%), Query Frame = 0


Query: 1   MVCGKRMAEG---------SEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTT 60
           MV GKR   G          E  + RK +  FF L G  TV DA P L +LDL G+ K  
Sbjct: 182 MVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGHEKEM 241

Query: 61  KEVFKELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELD--GYDADTVI 120
           K+  +ELD I+E W+E HRQ+++ +G      +   + VM  L +  +L    YDA+T I
Sbjct: 242 KQTRRELDVILERWIENHRQQRKVSGTKHN--DSDFVDVMLSLAEQGKLSHLQYDANTCI 301

Query: 121 KATCLTLISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVY 180
           K TCL LI GG++T   T+TWAISLLLNN+D L++VQ+E+DIHVG  R V +SDI  LVY
Sbjct: 302 KTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVY 361

Query: 181 LQAVIKETLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKF 240
           LQA+IKETLRLYPA PL G RE  EDCT+ GYNV  GT LI N+WKIQRDP+V+ EP++F
Sbjct: 362 LQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEF 421

Query: 241 KPERFLSSHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYD 300
           +PERF++    D+ DV+GQ+FEL PFG GRR CPG  +++ M  L LA  +HSF+V T  
Sbjct: 422 RPERFITGEAKDF-DVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVL 481

Query: 301 DEPVDMAANLGLTMRRVKPLHVLVKPRL 318
           D PVDM+ + GLT+ +  PL VL+ PRL
Sbjct: 482 DRPVDMSESPGLTITKATPLEVLINPRL 506

[Close]

BLAST of Cucsat.G13388 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 320.1 bits (819), Expect = 2.9e-86
Identity = 162/328 (49.39%), Postives = 213/328 (64.94%), Query Frame = 0


Query: 1   MVCGKR------MAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEV 60
           MV GKR      +    E +R  + +R F  L G  TVGD +PFLK+LDLGG+ K  K+ 
Sbjct: 216 MVVGKRCFGDVDVENKEEAKRFLENIRDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKC 275

Query: 61  FKELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCL 120
            K+ D ++ EWLEEHR+KK          E+  M  M L+LK   ++G+D DT+IKAT L
Sbjct: 276 AKKFDVMLNEWLEEHREKKGLGSEDKVVGERDFMDAMLLVLKDKPIEGFDVDTIIKATTL 335

Query: 121 TLISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVI 180
            LI GG+DT   T+TWA+ LLL +   L +++EEL+ ++G +R VNESDINKLVYL A+I
Sbjct: 336 ELILGGSDTTAGTLTWAMCLLLKHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAII 395

Query: 181 KETLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERF 240
           KETLRLYP  P S  RE TEDCTIGGY++  GT L+ N+WKI RDP VW +P +FKPERF
Sbjct: 396 KETLRLYPPAPFSSPREFTEDCTIGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERF 455

Query: 241 LSSHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVD 300
           LS+H    +DV+GQ+FEL PFG GRR+C G+ + L M   +LA+ +HSF++     E +D
Sbjct: 456 LSTHK--DVDVRGQNFELLPFGSGRRMCAGMSLGLHMVHYILANFLHSFEILNPSPESID 515

Query: 301 MAANLGLTMRRVKPLHVLVKPRLLATAY 323
           +   L     +  PL VLVKP L    Y
Sbjct: 516 VTEVLEFVTTKATPLEVLVKPCLSFKCY 541

[Close]

BLAST of Cucsat.G13388 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-84
Identity = 163/332 (49.10%), Postives = 216/332 (65.06%), Query Frame = 0


Query: 1   MVCGKRMAEG--------SEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTK 60
           MV GKR   G         E  +CRK +  FF L G  TV DA P L + D  G+ K  K
Sbjct: 194 MVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEMK 253

Query: 61  EVFKELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELD--GYDADTVIK 120
           +  +ELD I+E W+E HRQ+++ +G      +   + VM  L +  +     +DA T IK
Sbjct: 254 QTGRELDVILERWIENHRQQRKVSGTKHN--DSDFVDVMLSLAEQGKFSHLQHDAITSIK 313

Query: 121 ATCLTLISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYL 180
           +TCL LI GG++T   T+TWAISLLLNN+D L++ Q+E+DIHVG  R V +SDI  LVY+
Sbjct: 314 STCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYI 373

Query: 181 QAVIKETLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFK 240
           QA+IKETLRLYPAGPL G RE  EDCT+ GYNV  GT ++ N+WKIQRDPRV+ EP++F+
Sbjct: 374 QAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFR 433

Query: 241 PERFLSSHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDD 300
           PERF++    ++ DV+GQ+FEL PFG GRR CPG  +++ +  L LA  + SFDV T  D
Sbjct: 434 PERFITGEAKEF-DVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMD 493

Query: 301 EPVDMAANLGLTMRRVKPLHVLVKPRLLATAY 323
            PVDM  + GLT+ +  PL +L+ PRL    Y
Sbjct: 494 MPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

[Close]

BLAST of Cucsat.G13388 vs. NCBI nr
Match: XP_004147825.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59909.1 hypothetical protein Csa_002548 [Cucumis sativus])

HSP 1 Score: 649 bits (1675), Expect = 9.49e-232
Identity = 322/323 (99.69%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC 60
           MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC
Sbjct: 213 MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC 272

Query: 61  IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLTLISGG 120
           IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTV+KATCLTLISGG
Sbjct: 273 IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVVKATCLTLISGG 332

Query: 121 TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL 180
           TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL
Sbjct: 333 TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL 392

Query: 181 YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY 240
           YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY
Sbjct: 393 YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY 452

Query: 241 DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG 300
           DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG
Sbjct: 453 DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG 512

Query: 301 LTMRRVKPLHVLVKPRLLATAYA 323
           LTMRRVKPLHVLVKPRLLATAYA
Sbjct: 513 LTMRRVKPLHVLVKPRLLATAYA 535

[Close]

BLAST of Cucsat.G13388 vs. NCBI nr
Match: XP_038899207.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 555 bits (1429), Expect = 2.85e-194
Identity = 275/323 (85.14%), Postives = 298/323 (92.26%), Query Frame = 0


Query: 1   MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC 60
           MVCGKR+  GSEM+RCRKAMRGFFELAG++TVGDAIPFLKFLDLGGYLK TKEV KELDC
Sbjct: 221 MVCGKRLVGGSEMDRCRKAMRGFFELAGRITVGDAIPFLKFLDLGGYLKATKEVSKELDC 280

Query: 61  IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLTLISGG 120
           +MEEWLEEHRQKK+DAGA     E+ LMGVMP LL+GM+L GYDADTV KATCLTLISG 
Sbjct: 281 LMEEWLEEHRQKKKDAGAGE---EEDLMGVMPSLLEGMDLGGYDADTVNKATCLTLISGA 340

Query: 121 TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL 180
           TDTMTVTITWAISLLLNNQ+AL+ VQEELD HVGNKRLV+ESDI+KL YLQAVIKETLRL
Sbjct: 341 TDTMTVTITWAISLLLNNQEALKGVQEELDNHVGNKRLVDESDISKLEYLQAVIKETLRL 400

Query: 181 YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY 240
           YPAGPLSGAR+V++DCTIGGYNVA+GTHLITNIWKIQRDPRVW EPSKFKPERFLSSHNY
Sbjct: 401 YPAGPLSGARKVSQDCTIGGYNVAAGTHLITNIWKIQRDPRVWPEPSKFKPERFLSSHNY 460

Query: 241 DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG 300
           +++DVKGQHFEL PFGYGRR CPGLGISL+MTPLVLASLIHSF++ T  DEPVDMAANLG
Sbjct: 461 NHIDVKGQHFELCPFGYGRRSCPGLGISLLMTPLVLASLIHSFELRTRCDEPVDMAANLG 520

Query: 301 LTMRRVKPLHVLVKPRLLATAYA 323
           LTM RV PLHVLVKPRLLATAYA
Sbjct: 521 LTMHRVNPLHVLVKPRLLATAYA 540

[Close]

BLAST of Cucsat.G13388 vs. NCBI nr
Match: XP_023535452.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 509 bits (1311), Expect = 2.27e-176
Identity = 255/328 (77.74%), Postives = 282/328 (85.98%), Query Frame = 0


Query: 1   MVCGKRMAEGS-----EMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVF 60
           MVCGKR   GS     EMERCRKAMRGFF+L G+  VGDAIPFLK+LDLGGY K T +V+
Sbjct: 214 MVCGKRFVGGSCGDEREMERCRKAMRGFFDLGGKFIVGDAIPFLKWLDLGGYQKATNKVW 273

Query: 61  KELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLT 120
           KELDC+MEEWLEEHR+KKRD   +    E  LM  MP LL+G++L GYDADTV KATCLT
Sbjct: 274 KELDCLMEEWLEEHRRKKRDCSDADADGEHDLMAAMPSLLEGLDLAGYDADTVNKATCLT 333

Query: 121 LISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIK 180
           LISGGTDTM VT+TWAISLLLNNQ+ALR+V+EELDIHVG +R+V+ESDI+KLVYLQAVIK
Sbjct: 334 LISGGTDTMAVTLTWAISLLLNNQEALRKVEEELDIHVGKERVVDESDISKLVYLQAVIK 393

Query: 181 ETLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFL 240
           ETLRLYPAGPLSGAREVT+DCTIGGYNVA+GT+LITNIWKIQRDPR+W EPS+FKPERFL
Sbjct: 394 ETLRLYPAGPLSGAREVTQDCTIGGYNVAAGTYLITNIWKIQRDPRIWQEPSEFKPERFL 453

Query: 241 SSHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDM 300
           SSH +  MDVKGQHFELSPFGYGRR CPG GISL+MTPLVLASLIHSF + T  DE VDM
Sbjct: 454 SSHKH--MDVKGQHFELSPFGYGRRACPGQGISLLMTPLVLASLIHSFQLRTRCDEAVDM 513

Query: 301 AANLGLTMRRVKPLHVLVKPRLLATAYA 323
            ANLGLTM RV PL VLVKPRLL TAYA
Sbjct: 514 TANLGLTMHRVNPLQVLVKPRLLPTAYA 539

[Close]

BLAST of Cucsat.G13388 vs. NCBI nr
Match: XP_022937187.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 506 bits (1304), Expect = 3.10e-175
Identity = 255/328 (77.74%), Postives = 283/328 (86.28%), Query Frame = 0


Query: 1   MVCGKRMAEGS-----EMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVF 60
           MVCGKR   GS     EMERCRKAMRGFF+L G+  VGDAIPFLK+LDLGGY K T +V+
Sbjct: 221 MVCGKRFVGGSCGDEREMERCRKAMRGFFDLGGKFIVGDAIPFLKWLDLGGYQKATNKVW 280

Query: 61  KELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLT 120
           KELDC+MEEWLEEHR+KKRD   + G  E  LM VMP LL+G++L GYDADTV KATCLT
Sbjct: 281 KELDCLMEEWLEEHRRKKRDCSDADG--EHDLMAVMPSLLEGLDLAGYDADTVNKATCLT 340

Query: 121 LISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIK 180
           LISGGTDTM VT+TWAISLLLNNQ+ALR+V+EELDIHVG +R V+ESDI+KLVYLQAVIK
Sbjct: 341 LISGGTDTMAVTLTWAISLLLNNQEALRKVEEELDIHVGKERAVDESDISKLVYLQAVIK 400

Query: 181 ETLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFL 240
           ETLRLYPAGPLSGAREV++DCTIGGYNVA+GT+LITNIWKIQRDPR+W EPS+FKPERFL
Sbjct: 401 ETLRLYPAGPLSGAREVSQDCTIGGYNVAAGTYLITNIWKIQRDPRIWQEPSEFKPERFL 460

Query: 241 SSHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDM 300
           SSH +  MDVKGQHFELSPFGYGRR CPG GISL+MTPLVLASL+HSF + T  DE VDM
Sbjct: 461 SSHKH--MDVKGQHFELSPFGYGRRACPGQGISLLMTPLVLASLVHSFQLRTRRDEAVDM 520

Query: 301 AANLGLTMRRVKPLHVLVKPRLLATAYA 323
            ANLGLTM RV PL VLVKPRLL TAYA
Sbjct: 521 TANLGLTMHRVNPLQVLVKPRLLPTAYA 544

[Close]

BLAST of Cucsat.G13388 vs. NCBI nr
Match: XP_022156753.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 444 bits (1142), Expect = 8.08e-151
Identity = 223/323 (69.04%), Postives = 262/323 (81.11%), Query Frame = 0


Query: 1   MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC 60
           MVCGKR     EM+RCR+AMR FF+LAGQ  VGDA+PFL++LDLGGY K  K+  KELD 
Sbjct: 216 MVCGKRFVGEKEMKRCRRAMREFFDLAGQFMVGDALPFLRWLDLGGYQKAMKKTAKELDS 275

Query: 61  IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLTLISGG 120
           IMEEWL+EHR +K D+GA+A       M VM   L+G++L GYDA TV KATC+T+I+GG
Sbjct: 276 IMEEWLDEHR-RKADSGAAADGGPD-FMDVMLSTLEGIDLAGYDAATVNKATCMTIIAGG 335

Query: 121 TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL 180
           TDT T TI WAISLLLNN++AL+R QEELDIHVGNKRL++ESDI+KL YLQAV+KETLRL
Sbjct: 336 TDTTTETILWAISLLLNNREALKRAQEELDIHVGNKRLLDESDISKLEYLQAVVKETLRL 395

Query: 181 YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY 240
           YPAGPLSG RE + DCT+GGY+VA GT LITN+WKIQ DPRVW EP KFKPERFLSSH +
Sbjct: 396 YPAGPLSGIREFSRDCTVGGYDVAVGTRLITNLWKIQTDPRVWAEPLKFKPERFLSSHKH 455

Query: 241 DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG 300
             +DVKGQ+FE +PFG GRRVCPGLG  + MT LVLASLIHS ++ T  DEPVDM A+ G
Sbjct: 456 --LDVKGQNFEFTPFGCGRRVCPGLGFGIQMTQLVLASLIHSMELKTRFDEPVDMTADFG 515

Query: 301 LTMRRVKPLHVLVKPRLLATAYA 323
           +TM RV PL VL+KPRLLA+AYA
Sbjct: 516 ITMHRVNPLQVLIKPRLLASAYA 534

[Close]

BLAST of Cucsat.G13388 vs. ExPASy TrEMBL
Match: A0A0A0LFZ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852580 PE=3 SV=1)

HSP 1 Score: 649 bits (1675), Expect = 4.59e-232
Identity = 322/323 (99.69%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC 60
           MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC
Sbjct: 213 MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC 272

Query: 61  IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLTLISGG 120
           IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTV+KATCLTLISGG
Sbjct: 273 IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVVKATCLTLISGG 332

Query: 121 TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL 180
           TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL
Sbjct: 333 TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL 392

Query: 181 YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY 240
           YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY
Sbjct: 393 YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY 452

Query: 241 DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG 300
           DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG
Sbjct: 453 DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG 512

Query: 301 LTMRRVKPLHVLVKPRLLATAYA 323
           LTMRRVKPLHVLVKPRLLATAYA
Sbjct: 513 LTMRRVKPLHVLVKPRLLATAYA 535

[Close]

BLAST of Cucsat.G13388 vs. ExPASy TrEMBL
Match: A0A6J1FAF2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443559 PE=3 SV=1)

HSP 1 Score: 506 bits (1304), Expect = 1.50e-175
Identity = 255/328 (77.74%), Postives = 283/328 (86.28%), Query Frame = 0


Query: 1   MVCGKRMAEGS-----EMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVF 60
           MVCGKR   GS     EMERCRKAMRGFF+L G+  VGDAIPFLK+LDLGGY K T +V+
Sbjct: 221 MVCGKRFVGGSCGDEREMERCRKAMRGFFDLGGKFIVGDAIPFLKWLDLGGYQKATNKVW 280

Query: 61  KELDCIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLT 120
           KELDC+MEEWLEEHR+KKRD   + G  E  LM VMP LL+G++L GYDADTV KATCLT
Sbjct: 281 KELDCLMEEWLEEHRRKKRDCSDADG--EHDLMAVMPSLLEGLDLAGYDADTVNKATCLT 340

Query: 121 LISGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIK 180
           LISGGTDTM VT+TWAISLLLNNQ+ALR+V+EELDIHVG +R V+ESDI+KLVYLQAVIK
Sbjct: 341 LISGGTDTMAVTLTWAISLLLNNQEALRKVEEELDIHVGKERAVDESDISKLVYLQAVIK 400

Query: 181 ETLRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFL 240
           ETLRLYPAGPLSGAREV++DCTIGGYNVA+GT+LITNIWKIQRDPR+W EPS+FKPERFL
Sbjct: 401 ETLRLYPAGPLSGAREVSQDCTIGGYNVAAGTYLITNIWKIQRDPRIWQEPSEFKPERFL 460

Query: 241 SSHNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDM 300
           SSH +  MDVKGQHFELSPFGYGRR CPG GISL+MTPLVLASL+HSF + T  DE VDM
Sbjct: 461 SSHKH--MDVKGQHFELSPFGYGRRACPGQGISLLMTPLVLASLVHSFQLRTRRDEAVDM 520

Query: 301 AANLGLTMRRVKPLHVLVKPRLLATAYA 323
            ANLGLTM RV PL VLVKPRLL TAYA
Sbjct: 521 TANLGLTMHRVNPLQVLVKPRLLPTAYA 544

[Close]

BLAST of Cucsat.G13388 vs. ExPASy TrEMBL
Match: A0A6J1DRH5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111023576 PE=3 SV=1)

HSP 1 Score: 444 bits (1142), Expect = 3.91e-151
Identity = 223/323 (69.04%), Postives = 262/323 (81.11%), Query Frame = 0


Query: 1   MVCGKRMAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELDC 60
           MVCGKR     EM+RCR+AMR FF+LAGQ  VGDA+PFL++LDLGGY K  K+  KELD 
Sbjct: 216 MVCGKRFVGEKEMKRCRRAMREFFDLAGQFMVGDALPFLRWLDLGGYQKAMKKTAKELDS 275

Query: 61  IMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKGMELDGYDADTVIKATCLTLISGG 120
           IMEEWL+EHR +K D+GA+A       M VM   L+G++L GYDA TV KATC+T+I+GG
Sbjct: 276 IMEEWLDEHR-RKADSGAAADGGPD-FMDVMLSTLEGIDLAGYDAATVNKATCMTIIAGG 335

Query: 121 TDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKETLRL 180
           TDT T TI WAISLLLNN++AL+R QEELDIHVGNKRL++ESDI+KL YLQAV+KETLRL
Sbjct: 336 TDTTTETILWAISLLLNNREALKRAQEELDIHVGNKRLLDESDISKLEYLQAVVKETLRL 395

Query: 181 YPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSSHNY 240
           YPAGPLSG RE + DCT+GGY+VA GT LITN+WKIQ DPRVW EP KFKPERFLSSH +
Sbjct: 396 YPAGPLSGIREFSRDCTVGGYDVAVGTRLITNLWKIQTDPRVWAEPLKFKPERFLSSHKH 455

Query: 241 DYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAANLG 300
             +DVKGQ+FE +PFG GRRVCPGLG  + MT LVLASLIHS ++ T  DEPVDM A+ G
Sbjct: 456 --LDVKGQNFEFTPFGCGRRVCPGLGFGIQMTQLVLASLIHSMELKTRFDEPVDMTADFG 515

Query: 301 LTMRRVKPLHVLVKPRLLATAYA 323
           +TM RV PL VL+KPRLLA+AYA
Sbjct: 516 ITMHRVNPLQVLIKPRLLASAYA 534

[Close]

BLAST of Cucsat.G13388 vs. ExPASy TrEMBL
Match: A0A6J1F9J8 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443336 PE=3 SV=1)

HSP 1 Score: 423 bits (1087), Expect = 7.53e-143
Identity = 211/327 (64.53%), Postives = 259/327 (79.20%), Query Frame = 0


Query: 1   MVCGKR-MAEGSEMERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELD 60
           MVCGKR + + + + RCRKAMR FF+L GQ  VGDAIPFL++LDLGGY K  K   +ELD
Sbjct: 203 MVCGKRFIGDDAVVRRCRKAMREFFDLGGQFMVGDAIPFLRWLDLGGYEKAMKTTSRELD 262

Query: 61  CIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKG--MELDGYDADTVIKATCLTLI 120
           C++ +WLEEHR+++  + A+A  +E   M VM   L+   M+L GYDADTVIKATC+TLI
Sbjct: 263 CLITDWLEEHRRRRHSSTAAAADSEHDFMDVMLSSLEEGTMDLAGYDADTVIKATCMTLI 322

Query: 121 SGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKET 180
           +GGTDTMTVT+TWAIS LLNN++ LR+ QEELD+HVG +R+V+ESDI+KLVYLQA++KET
Sbjct: 323 AGGTDTMTVTVTWAISSLLNNREILRKAQEELDMHVGKERVVDESDISKLVYLQAIVKET 382

Query: 181 LRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSS 240
            RLYP GPL G RE ++DCT+GGYNVA+GT LITN+WKIQ DPRVW EP +FKPERFL  
Sbjct: 383 FRLYPPGPLGGVREFSQDCTVGGYNVAAGTRLITNLWKIQTDPRVWAEPLEFKPERFLGR 442

Query: 241 HNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAA 300
           H    +DV G++FEL PFGYGRR CPGLGI + MT L+LA+LIHSF++ T  DEPVDMAA
Sbjct: 443 HKQ--LDVSGKNFELGPFGYGRRACPGLGIGVQMTQLLLATLIHSFEIGTRFDEPVDMAA 502

Query: 301 -NLGLTMRRVKPLHVLVKPRLLATAYA 323
            N GLT RRV  L +LVKPRL  +AYA
Sbjct: 503 VNSGLTARRVSSLKLLVKPRLPPSAYA 527

[Close]

BLAST of Cucsat.G13388 vs. ExPASy TrEMBL
Match: A0A6J1ILZ8 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476806 PE=3 SV=1)

HSP 1 Score: 422 bits (1086), Expect = 1.07e-142
Identity = 213/327 (65.14%), Postives = 256/327 (78.29%), Query Frame = 0


Query: 1   MVCGKRMAEGSEM-ERCRKAMRGFFELAGQVTVGDAIPFLKFLDLGGYLKTTKEVFKELD 60
           MVCGKR     E+  RCRKAMR FF+L GQ  VGDAIPFL++LDLGGY K  K   +ELD
Sbjct: 203 MVCGKRFVGDDEVVRRCRKAMRDFFDLGGQFMVGDAIPFLRWLDLGGYEKAMKTTSRELD 262

Query: 61  CIMEEWLEEHRQKKRDAGASAGAAEKGLMGVMPLLLKG--MELDGYDADTVIKATCLTLI 120
           C++ EWLEEHR+K+  + A+A   E   M VM  +L+   M+L GYD DTVIKATC+TLI
Sbjct: 263 CLITEWLEEHRRKRHSSTAAAADGEHDFMDVMLSILEEGTMDLAGYDTDTVIKATCMTLI 322

Query: 121 SGGTDTMTVTITWAISLLLNNQDALRRVQEELDIHVGNKRLVNESDINKLVYLQAVIKET 180
           +GGTDTMTVT+TWAIS LLNN++ LR+ QEELD+HVG +R+V+ESDI+KLVYLQA++KET
Sbjct: 323 AGGTDTMTVTVTWAISSLLNNREILRKAQEELDMHVGKERVVDESDISKLVYLQAIVKET 382

Query: 181 LRLYPAGPLSGAREVTEDCTIGGYNVASGTHLITNIWKIQRDPRVWTEPSKFKPERFLSS 240
            RLYP GPL G RE ++DCT+GGYNVA+GT LITN+ KIQ DPRVW EP +FKPERFL  
Sbjct: 383 FRLYPPGPLGGVREFSQDCTVGGYNVAAGTRLITNLRKIQTDPRVWAEPLEFKPERFLGR 442

Query: 241 HNYDYMDVKGQHFELSPFGYGRRVCPGLGISLMMTPLVLASLIHSFDVTTYDDEPVDMAA 300
           H    +DV G++FEL PFGYGRR CPGLGI + MT L+LA+LIHSF++ T  DEPVDMAA
Sbjct: 443 HKQ--LDVSGKNFELGPFGYGRRACPGLGIGVQMTHLLLATLIHSFEIGTRFDEPVDMAA 502

Query: 301 -NLGLTMRRVKPLHVLVKPRLLATAYA 323
            N GLT RRV  L +LVKPRLL +AYA
Sbjct: 503 VNSGLTARRVNSLKLLVKPRLLPSAYA 527

The following BLAST results are available for this feature:

BLAST of Cucsat.G13388 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49859	5.3e-88	53.07	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
Q9SZ46	9.0e-88	51.52	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
O49396	5.8e-87	51.83	Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3	[more]
Q43068	2.9e-86	49.39	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
O49394	2.1e-84	49.10	Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=...	[more]

BLAST of Cucsat.G13388 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147825.1	9.49e-232	99.69	cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59909.1 hypothetical protein Csa_...	[more]
XP_038899207.1	2.85e-194	85.14	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_023535452.1	2.27e-176	77.74	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]
XP_022937187.1	3.10e-175	77.74	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_022156753.1	8.08e-151	69.04	cytochrome P450 CYP82D47-like [Momordica charantia]	[more]

BLAST of Cucsat.G13388 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFZ9	4.59e-232	99.69	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852580 PE=3 SV=1	[more]
A0A6J1FAF2	1.50e-175	77.74	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443559 PE=3...	[more]
A0A6J1DRH5	3.91e-151	69.04	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111023576 PE=...	[more]
A0A6J1F9J8	7.53e-143	64.53	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443336 PE=3...	[more]
A0A6J1ILZ8	1.07e-142	65.14	cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476806 PE=3 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 172..183 score: 53.22 coord: 119..136 score: 32.91 coord: 253..262 score: 55.62
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 5..304 e-value: 7.0E-67 score: 226.2
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 212..236 score: 39.98 coord: 108..125 score: 26.81 coord: 262..285 score: 28.5 coord: 128..154 score: 31.96 coord: 171..189 score: 37.08 coord: 252..262 score: 53.04
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..318 e-value: 1.3E-79 score: 270.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 10..316
None	No IPR available	PANTHER	PTHR47947:SF5	CYTOCHROME P450 FAMILY PROTEIN	coord: 1..322
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 1..322
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 255..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G13388.T1	Cucsat.G13388.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen