Cucsat.G13250 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G13250
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	aquaporin NIP2-1-like
Location	ctg1838: 3148844 .. 3152368 (+)
RNA-Seq Expression	Cucsat.G13250
Synteny	Cucsat.G13250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTATTTTCTAAGTTAGCTCAAAAAAAAAAAAAAAATAGTTACGTAAATTTCTCTCCCTATAATTCCTTGGCATTTCCTTATCAGAAATAAAAGAAATAGGAAGACAATAGGAAATTCACATTTTTGTTGCAAAAAGGCAAAGTGGCTAATTCTTATCTTTTAACAAAAATACCCCCAACAAATTTATTTATATGAAAATGAAAACCCAAAACAATATTCAAACCCTAAAACGAAAAAATAACATTTAATAGTATTTATTTTAATTTTTCGATTGGTCCCCAGCCACACTACACACTATACCGATTAATTTCGTTGCCTATAAGATATTTCCTTCCCTTCCCTTCCTTCAAAAATAAAACCATCTTACCAAAAATATTCAAACAAATAAATCACTCCCAATTTTTCAAATTCCCTAAAAAAAAAATGGCGAGACGCAGCGACGATGAAGAAGCCTTTATTGCCTTGGGAAACGAGTGCTCTGATCCTCAACCACCCTTGTTCCGCGACCGATTTGATGAACTTTACCCGCCCGAGTTCTCACGAAAGGTATGGATTTCTAGTTAATTTTGTTCTTGGTTGAATTTGAAGCGATTGGTTTGTGATCGGAGGAATTTTTGTGGTGGTTTAGCTTGTGGCGGAGGTGATCGCTACGTATTTGCTTGTGTTTGTGTCGTGTGGTGTGGCGGCATTGAGTGGGAGTGATGAACCGGTAGTGACGAAGCTTGGAGCTTCAATTACCTGTGGATTGATTGTGACGGTGATGATTTACTCCGTCGGACATATCTCCGGCGCTCATATGAATCCAGCGGTTACTATTGCTTTTGCTGCTGTTCGGCGATTTCCATGGAGACAGGTCATCTTCTTCTTCCTCCTCTGTTTTTCTCCCCAATTTTTATGCCAATTTACCATATTATCCTTTCGCTTTTTCATTTGTTATTTTTATGTTTTCTACAGATATTTTCTATTTTGAGAAAAGCCGTGCCAAATATTTAATTCACTTTTTTGCCTTTTTTTTTCTTCTTTTTTTAAGTTTTGATATTTAGTTTAAGAGTGGAAACCTATACTTAAAAAAGTTTCGAAATATTACATTTGTACCATTTAATTTTTTTATCATGTTATTTATTTTTAATTCTTTATTTTAAATTTAGTTTTCATTTACTCCCTACATTTCAATATTTAGTTTCAATTTTTGTTTGTTAATAGCACTGCTTGTTTTGTTATTCATACTTCAATAATTAAGTTCAAATGATTCTATCATAATTTTTTTACTTATAATTAATTAATTAATTTTTAGATATTAAAATTGATGTTGAGTATTTAAACCATATAAAATTCAAAAATCATGCTACATTTTCATGACTTAAAATACAGGAATTAAAAAGTTCCTTTTTTTTTTCTAAATGTAAAAAGAAAAAAAAAAGTTTTTCTTTCTTTCTTTTCAACATTAAAAGCATGCTTAGATATTGGTGGTTGAGTTGATAAGTGTACTGAGAATAGATGTGTATTAGGTAATATAATAAAAATGTCCAATAAAAAGACAAAACAATGTCTCCTTTCTTTTAACATTTAGAAAACAAAATCAAATTTATCATTATTTTTTTCAAGTTTACTAAATTAAAAATAGTAGAATTCTTATTTTAACTTTGGTACAAAATATTTGAACAATTATACACCGACAAATAATCACCCTGAATCTAGAGCTTTGGATTTCTAGTGCACTCTCTCATTTTCGAAAAATAAAATAAAATTATCAACATATTCTCTCTCTCTTTTTTTTTTTTCTTTTCAGAATACATACTCTTTTTTGCTCTATTTCACTCTTTTTTAAAAAATTTAAAAATTAAGAATATATACTCTTTTTCTTTTTCTTTTTTAGATTATATACTATCTTAATCTCTCTTTTTTTTCTGAAACTGTATTAAAAATTAAGATTATATATACTCTCTTACTTTTTTTTTTATTTAAGAATTATAATCTCTTACTTAAGAATTAAGAAAAAGGAACCCCCAATCAAATCAACAGTGATGGTGATATATATTATAAATGTCTATTTTATTGGACGGGCGGAAATTAATTATAATTTTTTTAAAATTGGAAATTTAGTGGTAATGATGTATGAGCTGCGCCACTTCATCAAAAAGAACAGTAAAAAATTATAAAAAAAGGGATGGAAAAGTGCAATTTATGCCACCTGAGAAGTGACGTGGATGAAGTGTACGGAAGATATTTCGATAAGTGTCCCCACCAGTTTGGCAGATATTATATATAAAAAGACTATTGGCAGATACTTTCTAAGGAAAGCAGCCTTAATTGCTTTTTAATTCACCAAAAGTCATCTTAATTGAGAATATGCTTTTCCATCTTTTCCAGTTTAGACCTTTCAAACCTTTTTTCTTTTTAACTCTTTTTCTATTTTATTTTCATATATATTTTTTACATGACTTAGTAATAATTGATGTATACTCTTATGGTTCGTGAATTGAATTTTATGCGGTTGATGCAGGTTCCACTTTATGCAGCAGCTCAATTAAGTGGAGCTACGTCTGCAGCCTTCACACTACGCATATTAATGGATCCAATTCAAGATTTAGGTACTACTTCACCACATGGACCGGCTTTGAAAGCACTTGTTATGGAGATTGTTGTCTCATTTTGTATGATGTTTGTCACTTCTGCGGTTGCCACCGACACCAAAGCTGTAAGAGCATCTTATTGTTGTTTACCTCTTACTCACAATCTTCTCTTAATTTCCTTTCGCTAAGGTTCACTGCTTTTTTATATGTAATTTTTGGCAGATAGGAGAGCTTGGAGGCGTAGCTGTGGGGTCAGCTGTATGTATCTCGTCCATCTTTGCTGGGTAAGCAAAGCAATTATAATTGTTTCTTTTTTTGGCTTTTTGTTCTTAGCTGGAAGTTTTGTGGGTATCTGGAAAAATCTTAAACAAGATTTTGTTTTGATTGAATTCCAAATTTGGACCCTATAGTTTGGAGAAAATTTGTTTATTTATGAGATTTTATCAAACTATAAAGCTGGGGTTAACTATGTGTAGGCCAATATCGGGTGGATCGATGAACCCAGCAAGATCAATAGGACCAGCCATTGCAAGTTCACGTTACGAAGGAATTTGGGTGTACATGATCGGTCCAGTTACAGGAACCTTGCTTGCATCATTTTCATATAATTTCATACGAGCCACTGAAAAACACACCCATTCACTCTCGTTACATTGACATCCAATCGATGGGATCATGCAGCTGGAACTTCAAGTCATACCAAAATCAACTAGTTCAATTATTGTATAAATTAATGTGTAGCTCCTATAATAAAAGTTGTAGTTTAAGTTGTAGCTTTAGAAAAACTTAAGGTAAGGTTAGCTTTAATTGTCTTGCAATTTATTTGTTTTTTTAGTTTCGAGAGAAGAATGAATCTGAAATAGAGATTCATTTTTCTTTTTATTACTCTTGTTATTTTACAAGTGTGAGTTTTGATCTGATTTTTTTTACAATAATCAATCATAAAATTGGTTCTTCTTTTCAAGTTAATTCTCT

Coding sequence (CDS)

ATGGCGAGACGCAGCGACGATGAAGAAGCCTTTATTGCCTTGGGAAACGAGTGCTCTGATCCTCAACCACCCTTGTTCCGCGACCGATTTGATGAACTTTACCCGCCCGAGTTCTCACGAAAGCTTGTGGCGGAGGTGATCGCTACGTATTTGCTTGTGTTTGTGTCGTGTGGTGTGGCGGCATTGAGTGGGAGTGATGAACCGGTAGTGACGAAGCTTGGAGCTTCAATTACCTGTGGATTGATTGTGACGGTGATGATTTACTCCGTCGGACATATCTCCGGCGCTCATATGAATCCAGCGGTTACTATTGCTTTTGCTGCTGTTCGGCGATTTCCATGGAGACAGGTCATCTTCTTCTTCCTCCTCTGTTTTTCTCCCCAATTTTTATGCCAATTTACCATATTATCCTTTCGCTTTTTCATTTGTTATTTTTATGTTTTCTACAGATATTTTCTATTTTGA

Protein sequence

MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVIFFFLLCFSPQFLCQFTILSFRFFICYFYVFYRYFLF

Homology

[Close]

BLAST of Cucsat.G13250 vs. ExPASy Swiss-Prot
Match: Q19KC1 (Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2)

HSP 1 Score: 283.5 bits (724), Expect = 2.4e-75
Identity = 140/244 (57.38%), Postives = 189/244 (77.46%), Query Frame = 0


Query: 14  LGNECSDPQPPLFRDR-FDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTK 73
           +G   +   PP + DR   +++PP   +K+V+EV++T+LLVFV+CG A + GSD+  +++
Sbjct: 20  IGTAQNSSMPPTYYDRSLADIFPPHLLKKVVSEVVSTFLLVFVTCGAAGIYGSDKDRISQ 79

Query: 74  LGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAA 133
           LG S+  GLIVTVMIY+VGHISGAHMNPAVT+AFA  R FPW QVP Y AAQ +G+  A+
Sbjct: 80  LGQSVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGSICAS 139

Query: 134 FTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGS 193
           F L+ ++ PI  LGTT+P GP   +LV+EI+V+F MMFVT AVATDT+A+GEL G+AVGS
Sbjct: 140 FVLKAVLHPIAVLGTTTPTGPHWHSLVIEIIVTFNMMFVTLAVATDTRAVGELAGLAVGS 199

Query: 194 AVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATE 253
           AVCI+SIFAG +SGGSMNPAR++GPA+AS+ Y G+W+Y +GPV GTL  +++Y +IR  E
Sbjct: 200 AVCITSIFAGAVSGGSMNPARTLGPALASNLYTGLWIYFLGPVLGTLSGAWTYTYIRFEE 259

Query: 254 KHTH 257
             +H
Sbjct: 260 APSH 263

[Close]

BLAST of Cucsat.G13250 vs. ExPASy Swiss-Prot
Match: Q6Z2T3 (Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.3e-73
Identity = 134/218 (61.47%), Postives = 179/218 (82.11%), Query Frame = 0


Query: 32  ELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYSVG 91
           + +PP   +K+V+EV+AT+LLVF++CG A +SGSD   +++LG SI  GLIVTVMIY+VG
Sbjct: 41  DFFPPHLLKKVVSEVVATFLLVFMTCGAAGISGSDLSRISQLGQSIAGGLIVTVMIYAVG 100

Query: 92  HISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDLGTTSPH 151
           HISGAHMNPAVT+AFA  R FPW QVP Y AAQ +GA  A+F L+ ++ P+  +GTT+P 
Sbjct: 101 HISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGAICASFVLKAVIHPVDVIGTTTPV 160

Query: 152 GPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPISGGSMNP 211
           GP   +LV+E++V+F MMFVT AVATDT+A+GEL G+AVGSAVCI+SIFAG ISGGSMNP
Sbjct: 161 GPHWHSLVVEVIVTFNMMFVTLAVATDTRAVGELAGLAVGSAVCITSIFAGAISGGSMNP 220

Query: 212 ARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIR 250
           AR++GPA+AS++++G+W+Y +GPV GTL  +++Y FIR
Sbjct: 221 ARTLGPALASNKFDGLWIYFLGPVMGTLSGAWTYTFIR 258

[Close]

BLAST of Cucsat.G13250 vs. ExPASy Swiss-Prot
Match: Q67WJ8 (Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.9e-73
Identity = 136/223 (60.99%), Postives = 178/223 (79.82%), Query Frame = 0


Query: 30  FDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYS 89
           F +++PP   +K+++EV+AT+LLVFV+CG A++ G D   +++LG S+  GLIVTVMIY+
Sbjct: 42  FADIFPPNLLKKVISEVVATFLLVFVTCGAASIYGEDMKRISQLGQSVVGGLIVTVMIYA 101

Query: 90  VGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDLGTTS 149
            GHISGAHMNPAVT++FA  R FPW QVP Y AAQ +GA  AAF LR ++ PI+ LGTT+
Sbjct: 102 TGHISGAHMNPAVTLSFAFFRHFPWIQVPFYWAAQFTGAMCAAFVLRAVLYPIEVLGTTT 161

Query: 150 PHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPISGGSM 209
           P GP   ALV+EIVV+F MMFVT AVATD++A+GEL G+AVGSAVCI+SIFAGP+SGGSM
Sbjct: 162 PTGPHWHALVIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSM 221

Query: 210 NPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATE 253
           NPAR++ PA+AS+ Y G+W+Y +GPV GTL  ++ Y +IR  E
Sbjct: 222 NPARTLAPAVASNVYTGLWIYFLGPVVGTLSGAWVYTYIRFEE 264

[Close]

BLAST of Cucsat.G13250 vs. ExPASy Swiss-Prot
Match: Q9AT74 (Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 5.6e-72
Identity = 131/221 (59.28%), Postives = 175/221 (79.19%), Query Frame = 0


Query: 32  ELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYSVG 91
           +++PP   +K+++EV+AT+LLVFV+CG A++ G D   +++LG S+  GLIVTVMIY+ G
Sbjct: 47  DIFPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNARISQLGQSVAGGLIVTVMIYATG 106

Query: 92  HISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDLGTTSPH 151
           HISGAHMNPAVT++FA  R FPW QVP Y AAQ +GA  AAF L+ ++ PI  +GTT+P 
Sbjct: 107 HISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAVLQPIAVIGTTTPS 166

Query: 152 GPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPISGGSMNP 211
           GP   AL +EIVV+F MMFVT AVATD++A+GEL G+AVGSAVCI+SIFAGP+SGGSMNP
Sbjct: 167 GPHWHALAIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNP 226

Query: 212 ARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATE 253
           AR++ PA+AS+ + G+W+Y +GPV GTL  ++ Y +IR  E
Sbjct: 227 ARTLAPAVASNVFTGLWIYFLGPVVGTLSGAWVYTYIRFEE 267

[Close]

BLAST of Cucsat.G13250 vs. ExPASy Swiss-Prot
Match: Q9ATN2 (Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 9.6e-72
Identity = 135/232 (58.19%), Postives = 180/232 (77.59%), Query Frame = 0


Query: 32  ELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDEPVVTKLGASITCGLIVTVMIYSVG 91
           +++PP   +K+++EV+AT+LLVFV+CG A++ G D   +++LG S+  GLIVTVMIY+ G
Sbjct: 44  DIFPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNRRISQLGQSVAGGLIVTVMIYATG 103

Query: 92  HISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSGATSAAFTLRILMDPIQDLGTTSPH 151
           HISGAHMNPAVT++FA  R FPW QVP Y AAQ +GA  AAF L+ ++ PI  +GTT+P 
Sbjct: 104 HISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAVLHPIAVIGTTTPS 163

Query: 152 GPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGGVAVGSAVCISSIFAGPISGGSMNP 211
           GP   AL++EIVV+F MMFVT AVATD++A+GEL G+AVGSAVCI+SIFAGP+SGGSMNP
Sbjct: 164 GPHWHALLIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNP 223

Query: 212 ARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNFIRATE----KHTHSLS 260
           AR++ PA+AS+ + G+W+Y +GPV GTL  ++ Y +IR  E    K T  LS
Sbjct: 224 ARTLAPAVASNVFTGLWIYFLGPVIGTLSGAWVYTYIRFEEAPAAKDTQRLS 275

[Close]

BLAST of Cucsat.G13250 vs. NCBI nr
Match: APT69294.1 (LSi6 [Cucumis sativus var. sativus] >KGN59571.1 hypothetical protein Csa_000858 [Cucumis sativus])

HSP 1 Score: 508 bits (1308), Expect = 2.88e-181
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120
           ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120

Query: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180
           AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK
Sbjct: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180

Query: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240
           AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL
Sbjct: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240

Query: 241 ASFSYNFIRATEKHTHSLSLH 261
           ASFSYNFIRATEKHTHSLSLH
Sbjct: 241 ASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250 vs. NCBI nr
Match: NP_001292702.1 (aquaporin NIP2-1-like [Cucumis sativus] >ACU29604.1 Si transport-like protein 2 [Cucumis sativus])

HSP 1 Score: 506 bits (1304), Expect = 1.17e-180
Identity = 260/261 (99.62%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120
           ALSGSDEPVVTKLGASITCGLIVTVMIYS GHISGAHMNPAVTIAFAAVRRFPWRQVPLY
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSAGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120

Query: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180
           AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK
Sbjct: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180

Query: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240
           AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL
Sbjct: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240

Query: 241 ASFSYNFIRATEKHTHSLSLH 261
           ASFSYNFIRATEKHTHSLSLH
Sbjct: 241 ASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250 vs. NCBI nr
Match: NP_001380719.1 (aquaporin NIP2-2 [Cucumis melo])

HSP 1 Score: 483 bits (1244), Expect = 1.64e-171
Identity = 246/261 (94.25%), Postives = 256/261 (98.08%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARR+D+EEAFIALGNECSDPQP LFRDRFDE+YPP FSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRNDNEEAFIALGNECSDPQPSLFRDRFDEVYPPGFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120
           ALSGSDE  V+KLGASITCGLIVTVMIY+VGHISGAHMNPAVTIAFAAVRRFPW+QVPLY
Sbjct: 61  ALSGSDEQEVSKLGASITCGLIVTVMIYAVGHISGAHMNPAVTIAFAAVRRFPWKQVPLY 120

Query: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180
           AAAQLSGATSAAFTLRIL+DPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK
Sbjct: 121 AAAQLSGATSAAFTLRILLDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180

Query: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240
           AIGELGG+AVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGP+TGTLL
Sbjct: 181 AIGELGGIAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPITGTLL 240

Query: 241 ASFSYNFIRATEKHTHSLSLH 261
           A+FSYNFIRATEKHTHSLS H
Sbjct: 241 AAFSYNFIRATEKHTHSLSSH 261

[Close]

BLAST of Cucsat.G13250 vs. NCBI nr
Match: XP_038906096.1 (aquaporin NIP2-2-like [Benincasa hispida])

HSP 1 Score: 421 bits (1083), Expect = 6.91e-147
Identity = 219/256 (85.55%), Postives = 234/256 (91.41%), Query Frame = 0


Query: 5   SDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSG 64
           S +EEA I  G+ECSDPQ   FRDRF ELYPPEFSRKLVAEVIATYLLVFV+CG AALS 
Sbjct: 13  SSNEEALI--GSECSDPQQSFFRDRFYELYPPEFSRKLVAEVIATYLLVFVTCGAAALSV 72

Query: 65  SDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQ 124
           SDE  V+KLGASIT GLIVTVMIY+VGHISGAHMNPAVT AFAAVRRFPW QVPLYAAAQ
Sbjct: 73  SDEREVSKLGASITGGLIVTVMIYAVGHISGAHMNPAVTFAFAAVRRFPWNQVPLYAAAQ 132

Query: 125 LSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGE 184
           LSGAT+AAFTLRIL+DPIQDLGTTSP GP  KAL+MEIVVSFCMMFVTSAVATDTKA+GE
Sbjct: 133 LSGATTAAFTLRILLDPIQDLGTTSPSGPIFKALLMEIVVSFCMMFVTSAVATDTKAVGE 192

Query: 185 LGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFS 244
           L G+AVGSAVCI+SIFAGPISGGSMNPARSIGPAIASSRYEGIWVY++GPV GTLL +FS
Sbjct: 193 LAGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYLVGPVAGTLLGAFS 252

Query: 245 YNFIRATEKHTHSLSL 260
           YNFIRATEKH+ SLSL
Sbjct: 253 YNFIRATEKHSRSLSL 266

[Close]

BLAST of Cucsat.G13250 vs. NCBI nr
Match: XP_023528272.1 (aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 408 bits (1049), Expect = 9.35e-142
Identity = 208/253 (82.21%), Postives = 230/253 (90.91%), Query Frame = 0


Query: 8   EEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDE 67
           EEAFI++G + SDP    FRDRF +L PPEFSRKLVAEVIATYLLVFV+CG AALS SDE
Sbjct: 11  EEAFISVGTDYSDPHQSFFRDRFQQLCPPEFSRKLVAEVIATYLLVFVTCGAAALSVSDE 70

Query: 68  PVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSG 127
             V+KLGAS+T GLIVTVMIY+VGHISGAHMNPAVT AFAAVRRFPW+QVPLYAAAQLSG
Sbjct: 71  RQVSKLGASMTGGLIVTVMIYAVGHISGAHMNPAVTFAFAAVRRFPWKQVPLYAAAQLSG 130

Query: 128 ATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGG 187
           ATSAAFTLRIL+DPI++LGTTSP GP  KAL++EIVVSFCMMFVTSAVATDTKAIGEL G
Sbjct: 131 ATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVATDTKAIGELAG 190

Query: 188 VAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNF 247
           +AVGS+VCI+SIFAGPISGGSMNPARSIGPAIASS YEGIWVY++GPVTGTLL ++SYNF
Sbjct: 191 IAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVTGTLLGAWSYNF 250

Query: 248 IRATEKHTHSLSL 260
           IR +EKH H LSL
Sbjct: 251 IRTSEKHGHQLSL 263

[Close]

BLAST of Cucsat.G13250 vs. ExPASy TrEMBL
Match: A0A1L7B7J9 (LSi6 OS=Cucumis sativus var. sativus OX=869827 GN=LSi6 PE=2 SV=1)

HSP 1 Score: 508 bits (1308), Expect = 1.39e-181
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120
           ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120

Query: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180
           AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK
Sbjct: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180

Query: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240
           AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL
Sbjct: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240

Query: 241 ASFSYNFIRATEKHTHSLSLH 261
           ASFSYNFIRATEKHTHSLSLH
Sbjct: 241 ASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250 vs. ExPASy TrEMBL
Match: A0A0A0LHR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G826650 PE=3 SV=1)

HSP 1 Score: 508 bits (1308), Expect = 1.39e-181
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120
           ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120

Query: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180
           AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK
Sbjct: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180

Query: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240
           AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL
Sbjct: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240

Query: 241 ASFSYNFIRATEKHTHSLSLH 261
           ASFSYNFIRATEKHTHSLSLH
Sbjct: 241 ASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250 vs. ExPASy TrEMBL
Match: G8XUV4 (Si transport-like protein 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 506 bits (1304), Expect = 5.67e-181
Identity = 260/261 (99.62%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120
           ALSGSDEPVVTKLGASITCGLIVTVMIYS GHISGAHMNPAVTIAFAAVRRFPWRQVPLY
Sbjct: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSAGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120

Query: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180
           AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK
Sbjct: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180

Query: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240
           AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL
Sbjct: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240

Query: 241 ASFSYNFIRATEKHTHSLSLH 261
           ASFSYNFIRATEKHTHSLSLH
Sbjct: 241 ASFSYNFIRATEKHTHSLSLH 261

[Close]

BLAST of Cucsat.G13250 vs. ExPASy TrEMBL
Match: A0A1S3B8P6 (aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487003 PE=3 SV=1)

HSP 1 Score: 483 bits (1244), Expect = 7.94e-172
Identity = 246/261 (94.25%), Postives = 256/261 (98.08%), Query Frame = 0


Query: 1   MARRSDDEEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVA 60
           MARR+D+EEAFIALGNECSDPQP LFRDRFDE+YPP FSRKLVAEVIATYLLVFVSCGVA
Sbjct: 1   MARRNDNEEAFIALGNECSDPQPSLFRDRFDEVYPPGFSRKLVAEVIATYLLVFVSCGVA 60

Query: 61  ALSGSDEPVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLY 120
           ALSGSDE  V+KLGASITCGLIVTVMIY+VGHISGAHMNPAVTIAFAAVRRFPW+QVPLY
Sbjct: 61  ALSGSDEQEVSKLGASITCGLIVTVMIYAVGHISGAHMNPAVTIAFAAVRRFPWKQVPLY 120

Query: 121 AAAQLSGATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180
           AAAQLSGATSAAFTLRIL+DPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK
Sbjct: 121 AAAQLSGATSAAFTLRILLDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTK 180

Query: 181 AIGELGGVAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLL 240
           AIGELGG+AVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGP+TGTLL
Sbjct: 181 AIGELGGIAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPITGTLL 240

Query: 241 ASFSYNFIRATEKHTHSLSLH 261
           A+FSYNFIRATEKHTHSLS H
Sbjct: 241 AAFSYNFIRATEKHTHSLSSH 261

[Close]

BLAST of Cucsat.G13250 vs. ExPASy TrEMBL
Match: A0A6J1J0H5 (aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482345 PE=3 SV=1)

HSP 1 Score: 405 bits (1041), Expect = 7.48e-141
Identity = 206/253 (81.42%), Postives = 229/253 (90.51%), Query Frame = 0


Query: 8   EEAFIALGNECSDPQPPLFRDRFDELYPPEFSRKLVAEVIATYLLVFVSCGVAALSGSDE 67
           EEAFI++G + SDP    FRDRF +L PPEFSRKLVAEVIATYLLVFV+CG AALS SDE
Sbjct: 11  EEAFISVGTDYSDPHQSFFRDRFQQLCPPEFSRKLVAEVIATYLLVFVTCGAAALSVSDE 70

Query: 68  PVVTKLGASITCGLIVTVMIYSVGHISGAHMNPAVTIAFAAVRRFPWRQVPLYAAAQLSG 127
             V+KLGAS+T GLIVTVMIY+VGHISGAHMNPAVT AFAAV+RFPW+QVPLYAAAQLSG
Sbjct: 71  RQVSKLGASMTGGLIVTVMIYAVGHISGAHMNPAVTFAFAAVKRFPWKQVPLYAAAQLSG 130

Query: 128 ATSAAFTLRILMDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVATDTKAIGELGG 187
           ATSAAFTLRIL+DPI++LGTTSP GP  KAL++EIVVSFCMMFVTSAVATDTKAIGEL G
Sbjct: 131 ATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVATDTKAIGELAG 190

Query: 188 VAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPVTGTLLASFSYNF 247
           +AVGS+VCI+SIFAGPISGGSMNPARSIGPAIASS YEGIWVY++GPV GTLL ++SYNF
Sbjct: 191 IAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVAGTLLGAWSYNF 250

Query: 248 IRATEKHTHSLSL 260
           IR +EKH H LSL
Sbjct: 251 IRTSEKHGHQLSL 263

The following BLAST results are available for this feature:

BLAST of Cucsat.G13250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q19KC1	2.4e-75	57.38	Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2	[more]
Q6Z2T3	2.3e-73	61.47	Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1	[more]
Q67WJ8	3.9e-73	60.99	Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1	[more]
Q9AT74	5.6e-72	59.28	Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1	[more]
Q9ATN2	9.6e-72	58.19	Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1	[more]

BLAST of Cucsat.G13250 vs. NCBI nr


Match Name	E-value	Identity	Description
APT69294.1	2.88e-181	100.00	LSi6 [Cucumis sativus var. sativus] >KGN59571.1 hypothetical protein Csa_000858 ...	[more]
NP_001292702.1	1.17e-180	99.62	aquaporin NIP2-1-like [Cucumis sativus] >ACU29604.1 Si transport-like protein 2 ...	[more]
NP_001380719.1	1.64e-171	94.25	aquaporin NIP2-2 [Cucumis melo]	[more]
XP_038906096.1	6.91e-147	85.55	aquaporin NIP2-2-like [Benincasa hispida]	[more]
XP_023528272.1	9.35e-142	82.21	aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G13250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1L7B7J9	1.39e-181	100.00	LSi6 OS=Cucumis sativus var. sativus OX=869827 GN=LSi6 PE=2 SV=1	[more]
A0A0A0LHR6	1.39e-181	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G826650 PE=3 SV=1	[more]
G8XUV4	5.67e-181	99.62	Si transport-like protein 2 OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A1S3B8P6	7.94e-172	94.25	aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487003 PE=3 SV=1	[more]
A0A6J1J0H5	7.48e-141	81.42	aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482345 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 75..93 score: 22.08 coord: 106..128 score: 40.01 coord: 31..50 score: 34.45 coord: 143..163 score: 33.09
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 2..160 e-value: 5.8E-44 score: 150.5
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 1..174 e-value: 2.9E-52 score: 179.0
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 1..170
None	No IPR available	PANTHER	PTHR45724:SF30	MAJOR INTRINSIC PROTEIN, AQUAPORIN-LIKE PROTEIN-RELATED	coord: 1..175
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 1..175
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 12..20

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G13250.T3	Cucsat.G13250.T3	mRNA
Cucsat.G13250.T2	Cucsat.G13250.T2	mRNA
Cucsat.G13250.T5	Cucsat.G13250.T5	mRNA
Cucsat.G13250.T1	Cucsat.G13250.T1	mRNA
Cucsat.G13250.T4	Cucsat.G13250.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015267	channel activity