Cucsat.G12964 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12964
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionEngB-type G domain-containing protein
Locationctg1838: 7995843 .. 7998735 (-)
RNA-Seq ExpressionCucsat.G12964
SyntenyCucsat.G12964
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGACAAAACGTTTTGGTTAGCATGATCGACAAGTCGTAGTCTCGAGGCAACTCGTACGACAAGTCGAACTCTTTATCAACTAAGCAGCCTTCTGTACATAGGCAAATGGTCCGACAAGAACCCACCGCATTTTCATCTTCAAAAAACTTCCACTCAGAGCCGAGCAAGTGCTCAAAGTCACAGAGATGGTTCTCTCTCACCTCTCTAGAATCAACAACTTTCTCTTTACTTCACCAATTCATCGAAATTTCAGCTTATTTGCAAAAACCAATAAACCCATTATCGCGGCAACAGGAGCCCCCATTCTCACATCGACCCCATTCCCCGTCCCCAAATCCACTCTCACAGCCGCCGAACCAATTCCTATCACCGAATTTACAGAGTATCCGGAAGAAACCCAGCTACCCATTTCCCCTGAGAAGCTATTTGTGCCGCCGGATACTGATATCTCCGGCGGCGGGGGGGCAAGAATCTTGAAGGGATCGAACATTGTGTTGGGTCGGTACGCAAGAGATGCGCAAGTTTCTCAGGCGGATTTTGTAAAGAGTAGTGTGAGAACTGAGGACTGCCCTTCTGATGGGCTTCCTGAATTTGCCCTTGTTGGGCGCTCGAATGTGGGGAAATCATCTCTGTTGAATTCACTCGTTCGGCGGAAGAAGCTCGCTCTCACCTCCAAGAAACCAGGTGAGTGGTTTGTTCTGACCCAAGTTCATCTTGATTAATTGGACTGTGAAGTCACTGTAGTCGCAAGGAAAAAATGTAGTTCCATCCAATAGAATCCATGAGAATTCTCTAGCTCTTGTCATTTTTTTTTTTTTTTTGTGTAATTTCAAACTATGTTCAATATGAATATGGATAAAAGAATTAGAGAGCTGTACAATGAAATAGAAGATAATTGTACAATATAGCTTGAACAGAACAAAAACCTTAACAGCTAGTGACCTAGTAGAAGATCCACTAATCAAAGCTTTGTAAGTTAGGATGATTCTTTATTTCCAGTGCTCTGCTTGGTTCTATTACATCTATGGTGCTGCAATGCCATTAATTTTGACCTTATAAAATATCTGCTCGATTAATTGGATCTCTCTCCATTGTCTGTCTTTATTCATTAAGTTTTTGTTGTTTTGAATTATCATTTTCCCCATGAGAATCCGACAGACATGTTAAACCCACATCCAATTTACGGATGTAAGTTAGGTACAGAGCAAAAAAGGAAGGAAGGAAGGGAGAACCTGCTCTTGTAGTCCGTCTTGGATACAAATATCATACATGAACATAAAGATGCTGAAGCTTTTTCTGATTTGCCTTATGATTGTCTATATAGAAGGAAAAGACAGAATGCTTTTGAATGTAAACACGTATATGTGAGCTAAATTATTGGAAGTGAAAGGACTTATTTGCATTATAGTTGTTGTTGGACCATATGCCTTTTATGTTATGACGTACAAGGGGTCTATATGTACTAAGGAATAGTGACAAGGGGGTTCGTTGGTGACCAAATATTATAGGCTCAAGTGGTTATCCTACGAAAACAGTTGAAGTGTGTGCAATGTTAAAGGAAAAAAGTGGGGGAGAAGAGTTTGGCATGTAAACATTGTTTATGTGAGCTTCAAACCTCTTGGATGTGTGAAATCTCTCGTACTCTTTAGACACAGACAGTCTTGCCCTATTGTTTTATTACTTAATCAGGTTTTTGGATATGGACATAAACATTGTTGTGATTTCTTGCAGGAAAGACACAGTGCATAAACCACTTTCGAATTAACGATAGTTGGTACTTGGTCGATTTGCCTGGATATGGGTATGTCTCAACTTCAAGTTGCGTCGATATTTGTCAAAAGATCTCTGACTCTTAAACTCAATTTTAAGTGATTTGCATAGCGATGGGATACAAGTTCACTCTCCAAATTTTTTCCACGTGCGACTCCTACATGAATGAATGTCAATGAGATTTGTTTCTTCCTCCTTTTTAGGTATGCAGCTGCACCACAGGAACTCCGAACAGATTGGAACAAGTTTACCAGAGACTATTTTCTGAATCGGTCCACATTGGTTTCAGTTTTCCTTCTCATAGATGCAAGCATTCCTGCCAAACCAATTGATCTTGACTATGCTAGTTGGTTGGGTCAGAATCAGGTAATATTCTCTTTGTTTTAGGGGTCTTATAGAAGGAAAATATTTAAATTGCATATCCTTTGTTTTCTTTAAAAGACAACTTTTAAAAAGTTGAAAGTATAGACGACCACAAAAAATAACAAAAACAAGAGCTGATACAAAAGCATAATAGATACCAAATTCTAGAAGTAGAGAGACTGAGGTGGTGAATAATTTCAGATTCTCTTCCAATTTCTATAAATAAAAAAAAGAGAGGATAATCCCTAAGCAATAAAGACAAACAGATTCTTGCAAGAGGTTCGAAGTATGCATTGCTACAGACCGTCAGACCTCCTACCGAGACAAGGCCTTTTCTTGGGAAGGCCAATTTCGTTTATGTCAAATTTGACAACTAAAGATTCTAATAGAACTCGAATATATCGAAAGATAAACGATAATGAAAATATAAGATAAACCAAGTGTTCAAGGTACTAGAACCCTCCCAGTCTTGAGATACTTTCAAGCCCTACTTCATATTCCAAAATATTGCTTATCCTTCCCCTACCTTACCTAGTGTATTTATAATCAAATTTACTTAACAAACTCCTTCAACTCATTGCTAATGTACTGTTAAGACTCTTCAACCTGAAACTCAGAAAAATCCCAACAATGGCCAAACAGAGGCAGCACTCTGCTATAATATATTGCAATATCAACCAAGAGTTCTACAACCTAACATACAATCAAAGATGAAAACAAGAAAGCAAAATACAAAATAAAGATGGAAATAAACTCAACTCCTTTGATAAGGCTGGAAACCTTCCCCTTTAAAGCTTACATTAGCTTTTACTGAAAGATACCTAATTCAGCAATCCTAAAAATTATTCCCTATTTATGCCAGCAAACCAAGTTGGCTCAGGTATTTCCTTCCTTTCCATTCAGCTCCCCAAATGAATTATTTTGGGAATTTCCCTTCTTGCCTCTTCTTTACAAGTGTGAAGGATTGGAGGTCTAGCACATACCTCTTAATATTCTTCCAATACCATTCTAATCAGATCCCTCACCTTTTGAAAACAAAGTTTGACAAGTGTGCATAATTGAGATTTCTTCTAAAACCCAAGTTCCAATTAGTATGGAAATCATCCAAAGTATAATATCGCTCTTCGTCATCATAGTAGGGACTAGGGAGATTATAGAGCCCGTGAAGAGATCTAAAGATTTACCTTCATTTAGAACAGTGTTTGAAGTTTCCAACTTTTTTTTCTCCATTACCAAGCTTAAAAGCAAATGCAGCTCAACTGTCCATTCTCCAAAGGAAATCACTTTCCCAAAACAAACCTCCCCATTCTTTTTAATAACAATGTGCCTAGTCATGACACGCTTCTATGGCAAGCATGTAATCTTCCAATTAATAAGGTGACTTCCTTTGATTTGCTAAAAGAGGAAAAAACCTAAGATAAGCTTTCTGTTGCATGCCTCCATTGATAACCCAAGTCTGATTGAAAAGTCTCGTTTGACTGATTGGGTCCTCCTTTCTCTTTGCTAATTTGCTCTTTTGATTGAGTACCCCCATTTCTCTTCTTAAGATAGTGCATCGTGATTTTTTAACAATGAATAGTAGAAATTTAGCTTTTACTTCAATTGTTGATGTCTTGGTTTTCAAGAAAAATTGACATAGAATGGTTTCTATAGATTCCAATGACGATAATATTCACGAAATGTGACAAACGGAAGAAGAAAAAGAATGGGGGAAAAAGACCAGAAGAAAATGTGAGCGACTTTCAGGAACTTATAAGTGGTTTTTTCCAAACGACTCCGCCGTGGATTATGACTAGCAGTGTCACCCATCAAGGTCGGGATGAAATTCTTCTACATATTGCTCAGCTGAGGAACTACTGGCTCAAGCACTGAATTTTTTTTTGCAGCCTCATCTTCTTGAATCAACCAGGTACAATGCATTGTTTGTTATGATTCCTAATGACGTATTGACACACGAAAAGTCGGTCTTATGATTTTGAACTTTTTACTATTAAAATTAACAGTAAATGCAAATGTTTCAGCTGGAAGATGCTTCTCAACGCTATTTGAATTGCTTGATCTTGACTCAAAATGAGAATGTGTTGATTGAATATGAATCTTGAATAGCCTAAGCTGTTTGATCAAGAACATGAATTTTGGAGAGTTAACAAAGCAACCATGCTAGCAATAATGGCTTAATAGTTGCACAATTTTCATTACGTTCCTACTTAAAAACTTCAAACATTCAATAGAATGGAAGATTGGTGGAAAGTTACTGTTATCTTGTGGGTGAATACTCTTCTCAGTTTGCTATTAGTTGTTAGAGCAATTGGATCCCATTTATTCATATTCTGGATAAAGGACAGCTTACCCCCTTTGTCCAAACTTGTAATATGATGTGTATAGTATAGCAAAGTTGTAGGGAAATTGGAAAAAAACAAGATTTTTTTGGGTGGGTAAAAGACTTTTGAGATGCTTTTTAAAAATAATGAATTTTGGGTAAAAAATTAGCATATGAAAAGTTCAAAGTGCCCTTTAGTTTTAGAAAAACCCTAACAGTGAATATGTGCAGTGATTCCTTCTCCCTCTCCCTCGCGTGCGATTCTTCTCAGTGAACGAGGTTGTGATGAACAACGAGCACGGAGCTTCAGACGGTGGCACAGCCAATGGAGGTGGAGACTTTGACGGTGGCGACTAAGTTGCGGTTCAACAACTTGGATGGCTGCTAAACTCTTCTATTCGACATCTTAATGAAGTGATACGATGTGTTGGGAGATTTAATATCTCAAGTGTACACTGTACAAAAAACTTAAATTAACGGCAGATTGTGATCCCAGACATTCAACTTTTAATAGCTAACATATGGTTTAGATTCTGTAACACTATGTCAAGAAAACAAATAACAGGACAAATACCATCTTCAATTGCTGTGTTTCATACATATCCAAGAAATAAGAATAATGAAAATAACTAACATGTTCAAAGTA

Coding sequence (CDS)

ATGGTTCTCTCTCACCTCTCTAGAATCAACAACTTTCTCTTTACTTCACCAATTCATCGAAATTTCAGCTTATTTGCAAAAACCAATAAACCCATTATCGCGGCAACAGGAGCCCCCATTCTCACATCGACCCCATTCCCCGTCCCCAAATCCACTCTCACAGCCGCCGAACCAATTCCTATCACCGAATTTACAGAGTATCCGGAAGAAACCCAGCTACCCATTTCCCCTGAGAAGCTATTTGTGCCGCCGGATACTGATATCTCCGGCGGCGGGGGGGCAAGAATCTTGAAGGGATCGAACATTGTGTTGGGTCGGTACGCAAGAGATGCGCAAGTTTCTCAGGCGGATTTTGTAAAGAGTAGTGTGAGAACTGAGGACTGCCCTTCTGATGGGCTTCCTGAATTTGCCCTTGTTGGGCGCTCGAATGTGGGGAAATCATCTCTGTTGAATTCACTCGTTCGGCGGAAGAAGCTCGCTCTCACCTCCAAGAAACCAGGAAAGACACAGTGCATAAACCACTTTCGAATTAACGATAGTTGGTACTTGGTCGATTTGCCTGGATATGGGTATGCAGCTGCACCACAGGAACTCCGAACAGATTGGAACAAGTTTACCAGAGACTATTTTCTGAATCGGTCCACATTGGTTTCAGTTTTCCTTCTCATAGATGCAAGCATTCCTGCCAAACCAATTGATCTTGACTATGCTAGTTGGTTGGGTCAGAATCAGATTCCAATGACGATAATATTCACGAAATGTGACAAACGGAAGAAGAAAAAGAATGGGGGAAAAAGACCAGAAGAAAATGTGAGCGACTTTCAGGAACTTATAAGTGGTTTTTTCCAAACGACTCCGCCGTGGATTATGACTAGCAGTGTCACCCATCAAGGTCGGGATGAAATTCTTCTACATATTGCTCAGCTGAGGAACTACTGGCTCAAGCACTGA

Protein sequence

MVLSHLSRINNFLFTSPIHRNFSLFAKTNKPIIAATGAPILTSTPFPVPKSTLTAAEPIPITEFTEYPEETQLPISPEKLFVPPDTDISGGGGARILKGSNIVLGRYARDAQVSQADFVKSSVRTEDCPSDGLPEFALVGRSNVGKSSLLNSLVRRKKLALTSKKPGKTQCINHFRINDSWYLVDLPGYGYAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTIIFTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLRNYWLKH
Homology
BLAST of Cucsat.G12964 vs. ExPASy Swiss-Prot
Match: O81004 (GTP-binding protein At2g22870 OS=Arabidopsis thaliana OX=3702 GN=EMB2001 PE=2 SV=2)

HSP 1 Score: 161.8 bits (408), Expect = 5.7e-39
Identity = 72/125 (57.60%), Postives = 94/125 (75.20%), Query Frame = 0

Query: 12  FRYAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMT 71
           + +A      +TDW+ FT+ YFLNR +LV V LLIDAS+P + IDLD A+WLG+N +PMT
Sbjct: 176 YGFAKVSDAAKTDWSAFTKGYFLNRDSLVCVLLLIDASVPPQKIDLDCANWLGRNNVPMT 235

Query: 72  IIFTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQ 131
            +FTKCDK K  K  GKRP+EN+  FQ++I   F+  PPWI+TSSV+  GRDE+LLH++Q
Sbjct: 236 FVFTKCDKMKATK--GKRPDENIKAFQQIIRENFKVHPPWILTSSVSGLGRDELLLHMSQ 295

Query: 132 LRNYW 137
           LRNYW
Sbjct: 296 LRNYW 298

BLAST of Cucsat.G12964 vs. ExPASy Swiss-Prot
Match: Q8A8T4 (Probable GTP-binding protein EngB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) OX=226186 GN=engB PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-15
Identity = 42/121 (34.71%), Postives = 69/121 (57.02%), Query Frame = 0

Query: 12  FRYAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMT 71
           + YA   Q+ +        DY L R  + ++F+LID+ +  + IDL++  WLG+N IP +
Sbjct: 79  YGYARRGQKGKDQIRTIIEDYILEREQMTNLFVLIDSRLEPQKIDLEFMEWLGENGIPFS 138

Query: 72  IIFTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQ 131
           IIFTK DK K     G R + N++++   +S  ++  PP+ ++SS    GR EIL +I  
Sbjct: 139 IIFTKADKLK-----GGRLKMNINNYLRELSKEWEELPPYFISSSENRTGRTEILDYIEN 194

Query: 132 L 133
           +
Sbjct: 199 I 194

BLAST of Cucsat.G12964 vs. ExPASy Swiss-Prot
Match: Q5LDL2 (Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=engB PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.7e-15
Identity = 42/121 (34.71%), Postives = 66/121 (54.55%), Query Frame = 0

Query: 12  FRYAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMT 71
           + YA   Q+ +        DY L R  + ++F+LID+ +  + IDL++  WLG+N IP  
Sbjct: 79  YGYARRGQKGQKQIRTIIEDYILEREQMTNLFVLIDSRLEPQKIDLEFMEWLGENGIPFA 138

Query: 72  IIFTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQ 131
           IIFTK DK K     G R + N+S +   +   ++  PP+ +TSS    GR E+L +I  
Sbjct: 139 IIFTKADKLK-----GGRLKINISAYLRELRKQWEELPPYFITSSEERLGRTEVLNYIES 194

Query: 132 L 133
           +
Sbjct: 199 I 194

BLAST of Cucsat.G12964 vs. ExPASy Swiss-Prot
Match: Q64UN4 (Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=engB PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.7e-15
Identity = 42/121 (34.71%), Postives = 66/121 (54.55%), Query Frame = 0

Query: 12  FRYAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMT 71
           + YA   Q+ +        DY L R  + ++F+LID+ +  + IDL++  WLG+N IP  
Sbjct: 79  YGYARRGQKGQEQIRTIIEDYILEREQMTNLFVLIDSRLEPQKIDLEFMEWLGENGIPFA 138

Query: 72  IIFTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQ 131
           IIFTK DK K     G R + N+S +   +   ++  PP+ +TSS    GR E+L +I  
Sbjct: 139 IIFTKADKLK-----GGRLKINISAYLRELRKQWEELPPYFITSSEERLGRTEVLNYIES 194

Query: 132 L 133
           +
Sbjct: 199 I 194

BLAST of Cucsat.G12964 vs. ExPASy Swiss-Prot
Match: A6LBY4 (Probable GTP-binding protein EngB OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152) OX=435591 GN=engB PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 7.5e-15
Identity = 41/123 (33.33%), Postives = 70/123 (56.91%), Query Frame = 0

Query: 12  FRYAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMT 71
           + +A   +E R    +   +Y L+R  L ++F+L+D    A+ IDL++  WLG++ +P +
Sbjct: 79  YGFAQRGKENREQLKRIIENYILDREQLTNLFVLLDCRHEAQKIDLEFMEWLGESGVPFS 138

Query: 72  IIFTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQ 131
           IIFTK DK  K      R +EN+  + + +   ++  PP  ++SS   +GRDEIL +I  
Sbjct: 139 IIFTKIDKISK-----GRLKENLKVYTDKLLETWEELPPIFLSSSEKKEGRDEILDYIES 196

Query: 132 LRN 135
           + N
Sbjct: 199 INN 196

BLAST of Cucsat.G12964 vs. NCBI nr
Match: XP_004136299.1 (GTP-binding protein At2g22870 [Cucumis sativus] >XP_031739374.1 GTP-binding protein At2g22870 [Cucumis sativus] >KGN60199.1 hypothetical protein Csa_002016 [Cucumis sativus])

HSP 1 Score: 266 bits (681), Expect = 3.80e-87
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII
Sbjct: 191 YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 250

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR
Sbjct: 251 FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 310

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 311 NYWLKH 316

BLAST of Cucsat.G12964 vs. NCBI nr
Match: KAA0038722.1 (GTP-binding protein [Cucumis melo var. makuwa] >TYK31334.1 GTP-binding protein [Cucumis melo var. makuwa])

HSP 1 Score: 264 bits (674), Expect = 4.39e-86
Identity = 124/126 (98.41%), Postives = 126/126 (100.00%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDL+YASWLGQNQIPMTII
Sbjct: 191 YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLEYASWLGQNQIPMTII 250

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLH+AQLR
Sbjct: 251 FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHMAQLR 310

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 311 NYWLKH 316

BLAST of Cucsat.G12964 vs. NCBI nr
Match: XP_038900017.1 (GTP-binding protein At2g22870 [Benincasa hispida])

HSP 1 Score: 264 bits (674), Expect = 4.83e-86
Identity = 124/126 (98.41%), Postives = 126/126 (100.00%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFT+DYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII
Sbjct: 194 YAAAPQELRTDWNKFTKDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 253

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR
Sbjct: 254 FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 313

Query: 134 NYWLKH 139
           NYWL+H
Sbjct: 314 NYWLEH 319

BLAST of Cucsat.G12964 vs. NCBI nr
Match: XP_023535583.1 (GTP-binding protein At2g22870-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 259 bits (663), Expect = 2.55e-84
Identity = 123/126 (97.62%), Postives = 124/126 (98.41%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFTR+YFLNRSTLVSVFLLIDASIPAK IDLDYASWLGQNQIPMTII
Sbjct: 198 YAAAPQELRTDWNKFTRNYFLNRSTLVSVFLLIDASIPAKQIDLDYASWLGQNQIPMTII 257

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDF ELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR
Sbjct: 258 FTKCDKRKKKKNGGKRPEENVSDFHELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 317

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 318 NYWLKH 323

BLAST of Cucsat.G12964 vs. NCBI nr
Match: XP_022936076.1 (GTP-binding protein At2g22870-like [Cucurbita moschata])

HSP 1 Score: 259 bits (663), Expect = 2.55e-84
Identity = 123/126 (97.62%), Postives = 124/126 (98.41%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFTR+YFLNRSTLVSVFLLIDASIPAK IDLDYASWLGQNQIPMTII
Sbjct: 198 YAAAPQELRTDWNKFTRNYFLNRSTLVSVFLLIDASIPAKQIDLDYASWLGQNQIPMTII 257

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDF ELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR
Sbjct: 258 FTKCDKRKKKKNGGKRPEENVSDFHELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 317

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 318 NYWLKH 323

BLAST of Cucsat.G12964 vs. ExPASy TrEMBL
Match: A0A0A0LGU2 (EngB-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G888580 PE=3 SV=1)

HSP 1 Score: 266 bits (681), Expect = 1.84e-87
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII
Sbjct: 191 YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 250

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR
Sbjct: 251 FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 310

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 311 NYWLKH 316

BLAST of Cucsat.G12964 vs. ExPASy TrEMBL
Match: A0A5A7T5H0 (GTP-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006340 PE=3 SV=1)

HSP 1 Score: 264 bits (674), Expect = 2.13e-86
Identity = 124/126 (98.41%), Postives = 126/126 (100.00%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDL+YASWLGQNQIPMTII
Sbjct: 191 YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLEYASWLGQNQIPMTII 250

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLH+AQLR
Sbjct: 251 FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHMAQLR 310

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 311 NYWLKH 316

BLAST of Cucsat.G12964 vs. ExPASy TrEMBL
Match: A0A6J1F6H7 (GTP-binding protein At2g22870-like OS=Cucurbita moschata OX=3662 GN=LOC111442787 PE=3 SV=1)

HSP 1 Score: 259 bits (663), Expect = 1.23e-84
Identity = 123/126 (97.62%), Postives = 124/126 (98.41%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFTR+YFLNRSTLVSVFLLIDASIPAK IDLDYASWLGQNQIPMTII
Sbjct: 198 YAAAPQELRTDWNKFTRNYFLNRSTLVSVFLLIDASIPAKQIDLDYASWLGQNQIPMTII 257

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDF ELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR
Sbjct: 258 FTKCDKRKKKKNGGKRPEENVSDFHELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 317

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 318 NYWLKH 323

BLAST of Cucsat.G12964 vs. ExPASy TrEMBL
Match: A0A6J1IKL5 (GTP-binding protein At2g22870-like OS=Cucurbita maxima OX=3661 GN=LOC111476474 PE=3 SV=1)

HSP 1 Score: 258 bits (660), Expect = 3.52e-84
Identity = 122/126 (96.83%), Postives = 124/126 (98.41%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQELRTDWNKFT++YFLNRSTLVSVFLLIDASIPAK IDLDYASWLGQNQIPMTII
Sbjct: 198 YAAAPQELRTDWNKFTKNYFLNRSTLVSVFLLIDASIPAKQIDLDYASWLGQNQIPMTII 257

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDF ELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR
Sbjct: 258 FTKCDKRKKKKNGGKRPEENVSDFHELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 317

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 318 NYWLKH 323

BLAST of Cucsat.G12964 vs. ExPASy TrEMBL
Match: A0A6J1CLQ0 (GTP-binding protein At2g22870 OS=Momordica charantia OX=3673 GN=LOC111012429 PE=3 SV=1)

HSP 1 Score: 256 bits (654), Expect = 2.52e-83
Identity = 120/126 (95.24%), Postives = 124/126 (98.41%), Query Frame = 0

Query: 14  YAAAPQELRTDWNKFTRDYFLNRSTLVSVFLLIDASIPAKPIDLDYASWLGQNQIPMTII 73
           YAAAPQ+LRTDWNKFT+DYFL RSTLVSVFLLIDASIPAK IDLDYASWLGQNQIPMTII
Sbjct: 194 YAAAPQDLRTDWNKFTKDYFLKRSTLVSVFLLIDASIPAKQIDLDYASWLGQNQIPMTII 253

Query: 74  FTKCDKRKKKKNGGKRPEENVSDFQELISGFFQTTPPWIMTSSVTHQGRDEILLHIAQLR 133
           FTKCDKRKKKKNGGKRPEENVSDFQELISGFF+TTPPWIMTSSVTHQGRDEILLHI+QLR
Sbjct: 254 FTKCDKRKKKKNGGKRPEENVSDFQELISGFFRTTPPWIMTSSVTHQGRDEILLHISQLR 313

Query: 134 NYWLKH 139
           NYWLKH
Sbjct: 314 NYWLKH 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O810045.7e-3957.60GTP-binding protein At2g22870 OS=Arabidopsis thaliana OX=3702 GN=EMB2001 PE=2 SV... [more]
Q8A8T41.2e-1534.71Probable GTP-binding protein EngB OS=Bacteroides thetaiotaomicron (strain ATCC 2... [more]
Q5LDL25.7e-1534.71Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain ATCC 25285 / D... [more]
Q64UN45.7e-1534.71Probable GTP-binding protein EngB OS=Bacteroides fragilis (strain YCH46) OX=2954... [more]
A6LBY47.5e-1533.33Probable GTP-binding protein EngB OS=Parabacteroides distasonis (strain ATCC 850... [more]
Match NameE-valueIdentityDescription
XP_004136299.13.80e-87100.00GTP-binding protein At2g22870 [Cucumis sativus] >XP_031739374.1 GTP-binding prot... [more]
KAA0038722.14.39e-8698.41GTP-binding protein [Cucumis melo var. makuwa] >TYK31334.1 GTP-binding protein [... [more]
XP_038900017.14.83e-8698.41GTP-binding protein At2g22870 [Benincasa hispida][more]
XP_023535583.12.55e-8497.62GTP-binding protein At2g22870-like [Cucurbita pepo subsp. pepo][more]
XP_022936076.12.55e-8497.62GTP-binding protein At2g22870-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LGU21.84e-87100.00EngB-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G888580... [more]
A0A5A7T5H02.13e-8698.41GTP-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G... [more]
A0A6J1F6H71.23e-8497.62GTP-binding protein At2g22870-like OS=Cucurbita moschata OX=3662 GN=LOC111442787... [more]
A0A6J1IKL53.52e-8496.83GTP-binding protein At2g22870-like OS=Cucurbita maxima OX=3661 GN=LOC111476474 P... [more]
A0A6J1CLQ02.52e-8395.24GTP-binding protein At2g22870 OS=Momordica charantia OX=3673 GN=LOC111012429 PE=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 136..253
e-value: 1.4E-21
score: 76.7
IPR019987GTP-binding protein, ribosome biogenesis, YsxCTIGRFAMTIGR03598TIGR03598coord: 116..306
e-value: 6.8E-58
score: 193.4
IPR019987GTP-binding protein, ribosome biogenesis, YsxCHAMAPMF_00321GTPase_EngBcoord: 121..310
score: 24.251057
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 107..312
e-value: 5.8E-53
score: 181.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 127..312
NoneNo IPR availablePANTHERPTHR11649MSS1/TRME-RELATED GTP-BINDING PROTEINcoord: 73..313
NoneNo IPR availablePANTHERPTHR11649:SF75PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 73..313
IPR030393EngB-type guanine nucleotide-binding (G) domainPROSITEPS51706G_ENGBcoord: 132..312
score: 36.012215
IPR030393EngB-type guanine nucleotide-binding (G) domainCDDcd01876YihA_EngBcoord: 135..309
e-value: 8.42639E-67
score: 205.052

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12964.T1Cucsat.G12964.T1mRNA
Cucsat.G12964.T5Cucsat.G12964.T5mRNA
Cucsat.G12964.T7Cucsat.G12964.T7mRNA
Cucsat.G12964.T3Cucsat.G12964.T3mRNA
Cucsat.G12964.T2Cucsat.G12964.T2mRNA
Cucsat.G12964.T6Cucsat.G12964.T6mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005525 GTP binding
molecular_function GO:0046872 metal ion binding