Cucsat.G12723 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12723
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRibonuclease MC-like
Locationctg1838: 3064178 .. 3065257 (-)
RNA-Seq ExpressionCucsat.G12723
SyntenyCucsat.G12723
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAAATGGTGGTAGCAATGGCAGGGGGAAGGAGATCAGATATTAATATTGTGTTATTGTTCGTGGTTACCTTAAGCATCAGCTTGTTTCCTATTATGAAATCACAACAATTTGATAAATTTTACTTTGTTCAACAATGGCCACCAGCTGTTTGCGATGGACGTACAGGAAAATGTGTAGGAAAAGGGATGTACTACTTTACAATTCATGGTGTGTGGCCTCAGAAAGGAGGAAAATCTGTAATTAATTGCCCTGGCACCCAATTTGATTTCAATAAGGTATTCTATTTTAGAATTCTCAAATTCACTCTATAAATCTTCCTCTATCTACATATATATATTATTTAAATAACTTTGAATAAAAATAACGATTTGAAACTAAATACATAGTTTAGTCTCAACACGACTCTTTTTAACATTACGTGGGAATGAGTTTCAGATTTCGAGTTTAGCAAACACTCTACATCAAATTATGAAGGATGTGATAAACGCAGACGACCAATTTTTATGGTCTCACGAGTGGAACAAACATGGAGTTTGCTCAGAAAGCAGGTACTCAATGAAACAATACTTCCAAATGGCGATAAATATGAAGTACAAGATCAACGTTCTTAGTGCCCTGAGGATGGGTGGGATAACCCCAAACAACCACTTGAAGGCCAAACAAAGAGTAGAGGGGGCGATGTTCACTGCATATCACGCATATCCTCTTCTTCGATGTAAAAAAGATTCATCTGGCCAATCCCTATTGACGGAGGTTGTTATGTGCTTTGACAATGATGGTGTCACCCTTCTAAACTGCACTACAACGAAATCCAATTGTGATGCAGACGTTCTCTTTTGATAACATATGTAAACTCTTCAATTCTCATGCATGCCAATAATAATGTTATTAGTTAACAATCCATGCATGTGCTTAATTCCATGCATAAACATAAGAAATAAAATATATATGTGTCATGTGGTTATCTATCTATAATTAAAATAAGTTGGTGCTATGCTTCGTCACCAATATTTGTCTCCAAGTTTATATAATATACTGTGTGTTTAAAGTTGTTGTCTTCTGTAATTTAAAGATAC

Coding sequence (CDS)

AATAAAATGGTGGTAGCAATGGCAGGGGGAAGGAGATCAGATATTAATATTGTGTTATTGTTCGTGGTTACCTTAAGCATCAGCTTGTTTCCTATTATGAAATCACAACAATTTGATAAATTTTACTTTGTTCAACAATGGCCACCAGCTGTTTGCGATGGACGTACAGGAAAATGTGTAGGAAAAGGGATGTACTACTTTACAATTCATGGTGTGTGGCCTCAGAAAGGAGGAAAATCTGTAATTAATTGCCCTGGCACCCAATTTGATTTCAATAAGATTTCGAGTTTAGCAAACACTCTACATCAAATTATGAAGGATGTGATAAACGCAGACGACCAATTTTTATGGTCTCACGAGTGGAACAAACATGGAGTTTGCTCAGAAAGCAGGTACTCAATGAAACAATACTTCCAAATGGCGATAAATATGAAGTACAAGATCAACGTTCTTAGTGCCCTGAGGATGGGTGGGATAACCCCAAACAACCACTTGAAGGCCAAACAAAGAGTAGAGGGGGCGATGTTCACTGCATATCACGCATATCCTCTTCTTCGATGTAAAAAAGATTCATCTGGCCAATCCCTATTGACGGAGGTTGTTATGTGCTTTGACAATGATGGTGTCACCCTTCTAAACTGCACTACAACGAAATCCAATTGTGATGCAGACGTTCTCTTTTGA

Protein sequence

NKMVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF
Homology
BLAST of Cucsat.G12723 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-45
Identity = 85/193 (44.04%), Postives = 124/193 (64.25%), Query Frame = 0

Query: 38  FDKFYFVQQWPPAVCD-GRTGKCVGKGMYYFTIHGVWPQKGGKSVINCP-GTQFDFNKIS 97
           FD F+FVQQWPPAVC   ++G C G G+  FTIHG+WPQ  G S+ NCP G+ FD  KIS
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 98  SLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKYKINVLSALR 157
            L + L+ +  +V+ A++Q  WSHEW KHG CSES ++   YF++A++M+   +++ ALR
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 158 MGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQ-SLLTEVVMCFDNDGVTLLNC 217
                PN   K++Q ++G +   +  +P LRC+ D   + S L +VV CF  DG TL++C
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 218 TTTKSNCDADVLF 228
             T+  C A+ +F
Sbjct: 181 --TRDTCGANFIF 191

BLAST of Cucsat.G12723 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.0e-30
Identity = 75/221 (33.94%), Postives = 113/221 (51.13%), Query Frame = 0

Query: 16  NIVLLFVVTLSISLFPI-MKSQQFDKFYFVQQWPPAVCDGRTGKCV---GKGMYYFTIHG 75
           ++ L+ ++ +  S+F     S+ FD FYFVQQWP + CD +   C    GK    F IHG
Sbjct: 7   SLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHG 66

Query: 76  VWPQ-KGGKSVINCPGTQ-FDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVC 135
           +WP  K G    NC  ++ FD + IS L  ++ +    +   +   +  W HEW KHG C
Sbjct: 67  LWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 126

Query: 136 SESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRC 195
           SES     +YFQ A+N+K K N+L AL   GI P+    + + +  ++  +    P + C
Sbjct: 127 SESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVEC 186

Query: 196 KKDSSGQSLLTEVVMCFDNDGVTLLNCTT-TKSNCDADVLF 228
            +D SG S L +V +C D  G  L+ C       C A++ F
Sbjct: 187 NRDGSGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEF 227

BLAST of Cucsat.G12723 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.7e-29
Identity = 75/216 (34.72%), Postives = 106/216 (49.07%), Query Frame = 0

Query: 20  LFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC---VGKGMYYFTIHGVWPQ- 79
           +F++ L   L+    +Q FD FYFV QWP A CD R   C    GK    F IHG+WP  
Sbjct: 5   IFILALQ-QLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNY 64

Query: 80  KGGKSVINC-PGTQFDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSESRY 139
           K G    NC P ++FD  ++S L + L +    +   + D    W+HEW KHG C+ES  
Sbjct: 65  KTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL 124

Query: 140 SMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSS 199
               YF+  + +K K N+L AL   GI P++     + +E  +       P + C KDSS
Sbjct: 125 DQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSS 184

Query: 200 GQSLLTEVVMCFDNDGVTLLNC-TTTKSNCDADVLF 228
             S L ++ +C D      +NC       CD+ V F
Sbjct: 185 HNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQF 219

BLAST of Cucsat.G12723 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 4.8e-27
Identity = 69/205 (33.66%), Postives = 111/205 (54.15%), Query Frame = 0

Query: 18  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC---VGKGMYYFTIHGVWP 77
           ++L ++T  +S+  +  ++ FD FYFVQQWP + CD +   C    GK    F IHG+WP
Sbjct: 11  LILLIITQCLSV--LNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWP 70

Query: 78  QKG-GKSVINC-PGTQFDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSES 137
               G    NC P + +D ++IS L +++ Q    +   +      WSHEW KHG C+ES
Sbjct: 71  NNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAES 130

Query: 138 RYSMKQ-YFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKK 197
             + +  YF+ A+++K +I++LS L+   I P+        +  A+ +A    P ++C  
Sbjct: 131 VLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNV 190

Query: 198 DSSGQSLLTEVVMCFDNDGVTLLNC 215
           D SG S L +V +C D  G +L+ C
Sbjct: 191 DQSGNSQLYQVYICVDGSGSSLIEC 213

BLAST of Cucsat.G12723 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 3.4e-25
Identity = 70/205 (34.15%), Postives = 102/205 (49.76%), Query Frame = 0

Query: 18  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKC---VGKGMYYFTIHGVWP 77
           +L+ ++ +   L   + SQ FD FYFVQQWP + CD R   C    GK    F+IHG+WP
Sbjct: 8   LLIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDFSIHGLWP 67

Query: 78  Q-KGGKSVINCP-GTQFDFNKISSLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSES 137
             K GK   NC   +  D ++ S L +T+ +    +   ++D    WSHEW KHG C  S
Sbjct: 68  NYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTC--S 127

Query: 138 RYSMKQYFQMAINMKYKINVLSALRMGGITP-NNHLKAKQRVEGAMFTAYHAYPLLRCKK 197
             +   YFQ A++ K K N+L  L   GI P N      + ++ A+       P + C  
Sbjct: 128 ALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECNV 187

Query: 198 DSSGQSLLTEVVMCFDNDGVTLLNC 215
           DS G   L +V +C D+     ++C
Sbjct: 188 DSQGNHQLYQVYLCVDSSASKFIDC 210

BLAST of Cucsat.G12723 vs. NCBI nr
Match: XP_004147497.3 (ribonuclease MC [Cucumis sativus] >KAE8651218.1 hypothetical protein Csa_002248 [Cucumis sativus])

HSP 1 Score: 462 bits (1190), Expect = 1.90e-164
Identity = 224/225 (99.56%), Postives = 225/225 (100.00%), Query Frame = 0

Query: 3   MVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGK 62
           MVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGK
Sbjct: 1   MVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGK 60

Query: 63  GMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWN 122
           GMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWN
Sbjct: 61  GMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWN 120

Query: 123 KHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAY 182
           KHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAY+AY
Sbjct: 121 KHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAY 180

Query: 183 PLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
           PLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF
Sbjct: 181 PLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 225

BLAST of Cucsat.G12723 vs. NCBI nr
Match: KAA0053765.1 (ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25637.1 ribonuclease MC-like [Cucumis melo var. makuwa])

HSP 1 Score: 343 bits (880), Expect = 1.97e-117
Identity = 160/209 (76.56%), Postives = 177/209 (84.69%), Query Frame = 0

Query: 19  LLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGG 78
           LLFVVTLSISLFP MKSQ FD FYFVQQWPPAVC GR+G+CVG+G  YFTIHGVWPQKGG
Sbjct: 2   LLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKGG 61

Query: 79  KSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYF 138
           +S++NCPGT+FD N+ISSL NTLHQ   DVIN  DQ  W HEWNKHG CSES+Y M  YF
Sbjct: 62  QSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKYQMLPYF 121

Query: 139 QMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLT 198
           QMAINMKYKI+VL A+R+GG+ PNNH KAKQRVE  +F AYHAYPLLRCKKDS+GQ LLT
Sbjct: 122 QMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLLT 181

Query: 199 EVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
           EVVMCF +DGVTLLNCT +KS C AD LF
Sbjct: 182 EVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

BLAST of Cucsat.G12723 vs. NCBI nr
Match: KAA0053767.1 (ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25635.1 ribonuclease MC-like [Cucumis melo var. makuwa])

HSP 1 Score: 342 bits (876), Expect = 8.00e-117
Identity = 159/209 (76.08%), Postives = 177/209 (84.69%), Query Frame = 0

Query: 19  LLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGG 78
           LLFVVTLSISLFP MKSQ FD FYFVQQWPPAVC GR+G+CVG+G  YFTIHGVWPQKGG
Sbjct: 2   LLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKGG 61

Query: 79  KSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYF 138
           +S++NCPGT+FD N+ISSL NTLHQ   DVIN  DQ  W HEWNKHG CSES++ M  YF
Sbjct: 62  QSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKFQMLPYF 121

Query: 139 QMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLT 198
           QMAINMKYKI+VL A+R+GG+ PNNH KAKQRVE  +F AYHAYPLLRCKKDS+GQ LLT
Sbjct: 122 QMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLLT 181

Query: 199 EVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
           EVVMCF +DGVTLLNCT +KS C AD LF
Sbjct: 182 EVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

BLAST of Cucsat.G12723 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 263 bits (673), Expect = 7.86e-86
Identity = 122/210 (58.10%), Postives = 159/210 (75.71%), Query Frame = 0

Query: 18  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKG 77
           V+ FV  L+I LFPI+KSQ FD F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKG
Sbjct: 9   VVFFVFVLTI-LFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKG 68

Query: 78  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 137
           G+SV NC G QFDF KI+ L N L+ +  +V+  +++F W HEWNKHG+CSES++   +Y
Sbjct: 69  GRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKY 128

Query: 138 FQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLL 197
           FQ AINM++ I++LS LR GG+ PN   KAKQRVE A+ + +   P+LRCKK S+GQ LL
Sbjct: 129 FQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLL 188

Query: 198 TEVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
           TE+VMCFD+DGVTL+NC   +SNC    +F
Sbjct: 189 TEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

BLAST of Cucsat.G12723 vs. NCBI nr
Match: XP_022155091.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 262 bits (669), Expect = 3.08e-85
Identity = 123/217 (56.68%), Postives = 163/217 (75.12%), Query Frame = 0

Query: 11  RRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIH 70
           + S   IV +FV+ ++I L PI+KSQ FD F+FVQQWPPAVC  + G+CVG+G+  FTIH
Sbjct: 2   KTSRREIVFMFVLAITIFL-PIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRTFTIH 61

Query: 71  GVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSES 130
           GVWPQKGG SV+NCPGT FDF KISSL   L+++  +VI  D++F WSHEWNKHGVCSES
Sbjct: 62  GVWPQKGGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSES 121

Query: 131 RYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKD 190
           +++MK YFQMAINM+  I++++AL++GG+ PN   K+KQRV+ A+ T +   P+LRC+  
Sbjct: 122 KFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGT 181

Query: 191 SSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
            S   L+ E+VMCFD+DGVTL+NC    SNC    +F
Sbjct: 182 GSNIRLI-EIVMCFDDDGVTLINCNPVSSNCANSFIF 216

BLAST of Cucsat.G12723 vs. ExPASy TrEMBL
Match: A0A0A0LHQ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G825060 PE=3 SV=1)

HSP 1 Score: 457 bits (1176), Expect = 1.08e-162
Identity = 220/221 (99.55%), Postives = 221/221 (100.00%), Query Frame = 0

Query: 7   MAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYY 66
           MAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYY
Sbjct: 1   MAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYY 60

Query: 67  FTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGV 126
           FTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGV
Sbjct: 61  FTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGV 120

Query: 127 CSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLR 186
           CSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAY+AYPLLR
Sbjct: 121 CSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLR 180

Query: 187 CKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
           CKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF
Sbjct: 181 CKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 221

BLAST of Cucsat.G12723 vs. ExPASy TrEMBL
Match: A0A5A7UJ53 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007970 PE=3 SV=1)

HSP 1 Score: 343 bits (880), Expect = 9.52e-118
Identity = 160/209 (76.56%), Postives = 177/209 (84.69%), Query Frame = 0

Query: 19  LLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGG 78
           LLFVVTLSISLFP MKSQ FD FYFVQQWPPAVC GR+G+CVG+G  YFTIHGVWPQKGG
Sbjct: 2   LLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKGG 61

Query: 79  KSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYF 138
           +S++NCPGT+FD N+ISSL NTLHQ   DVIN  DQ  W HEWNKHG CSES+Y M  YF
Sbjct: 62  QSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKYQMLPYF 121

Query: 139 QMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLT 198
           QMAINMKYKI+VL A+R+GG+ PNNH KAKQRVE  +F AYHAYPLLRCKKDS+GQ LLT
Sbjct: 122 QMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLLT 181

Query: 199 EVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
           EVVMCF +DGVTLLNCT +KS C AD LF
Sbjct: 182 EVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

BLAST of Cucsat.G12723 vs. ExPASy TrEMBL
Match: A0A5D3DQ69 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007950 PE=3 SV=1)

HSP 1 Score: 342 bits (876), Expect = 3.87e-117
Identity = 159/209 (76.08%), Postives = 177/209 (84.69%), Query Frame = 0

Query: 19  LLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGG 78
           LLFVVTLSISLFP MKSQ FD FYFVQQWPPAVC GR+G+CVG+G  YFTIHGVWPQKGG
Sbjct: 2   LLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKGG 61

Query: 79  KSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYF 138
           +S++NCPGT+FD N+ISSL NTLHQ   DVIN  DQ  W HEWNKHG CSES++ M  YF
Sbjct: 62  QSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKFQMLPYF 121

Query: 139 QMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLT 198
           QMAINMKYKI+VL A+R+GG+ PNNH KAKQRVE  +F AYHAYPLLRCKKDS+GQ LLT
Sbjct: 122 QMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLLT 181

Query: 199 EVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
           EVVMCF +DGVTLLNCT +KS C AD LF
Sbjct: 182 EVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

BLAST of Cucsat.G12723 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 263 bits (673), Expect = 3.80e-86
Identity = 122/210 (58.10%), Postives = 159/210 (75.71%), Query Frame = 0

Query: 18  VLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKG 77
           V+ FV  L+I LFPI+KSQ FD F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKG
Sbjct: 9   VVFFVFVLTI-LFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKG 68

Query: 78  GKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 137
           G+SV NC G QFDF KI+ L N L+ +  +V+  +++F W HEWNKHG+CSES++   +Y
Sbjct: 69  GRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKY 128

Query: 138 FQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLL 197
           FQ AINM++ I++LS LR GG+ PN   KAKQRVE A+ + +   P+LRCKK S+GQ LL
Sbjct: 129 FQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLL 188

Query: 198 TEVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
           TE+VMCFD+DGVTL+NC   +SNC    +F
Sbjct: 189 TEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

BLAST of Cucsat.G12723 vs. ExPASy TrEMBL
Match: A0A6J1DQN7 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1)

HSP 1 Score: 262 bits (669), Expect = 1.49e-85
Identity = 123/217 (56.68%), Postives = 163/217 (75.12%), Query Frame = 0

Query: 11  RRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIH 70
           + S   IV +FV+ ++I L PI+KSQ FD F+FVQQWPPAVC  + G+CVG+G+  FTIH
Sbjct: 2   KTSRREIVFMFVLAITIFL-PIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRTFTIH 61

Query: 71  GVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSES 130
           GVWPQKGG SV+NCPGT FDF KISSL   L+++  +VI  D++F WSHEWNKHGVCSES
Sbjct: 62  GVWPQKGGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSES 121

Query: 131 RYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKD 190
           +++MK YFQMAINM+  I++++AL++GG+ PN   K+KQRV+ A+ T +   P+LRC+  
Sbjct: 122 KFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGT 181

Query: 191 SSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 227
            S   L+ E+VMCFD+DGVTL+NC    SNC    +F
Sbjct: 182 GSNIRLI-EIVMCFDDDGVTLINCNPVSSNCANSFIF 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P235401.3e-4544.04Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1[more]
P428136.0e-3033.94Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
P428156.7e-2934.72Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1[more]
P800224.8e-2733.66Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
P801963.4e-2534.15Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_004147497.31.90e-16499.56ribonuclease MC [Cucumis sativus] >KAE8651218.1 hypothetical protein Csa_002248 ... [more]
KAA0053765.11.97e-11776.56ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25637.1 ribonuclease MC-like... [more]
KAA0053767.18.00e-11776.08ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25635.1 ribonuclease MC-like... [more]
XP_004147499.17.86e-8658.10ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C... [more]
XP_022155091.13.08e-8556.68ribonuclease MC-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0LHQ91.08e-16299.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G825060 PE=3 SV=1[more]
A0A5A7UJ539.52e-11876.56Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352... [more]
A0A5D3DQ693.87e-11776.08Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352... [more]
A0A0A0LC373.80e-8658.10Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1[more]
A0A6J1DQN71.49e-8556.68ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 38..214
e-value: 8.0E-39
score: 133.7
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 20..222
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 38..226
e-value: 1.5E-49
score: 170.6
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 38..227
NoneNo IPR availablePANTHERPTHR11240:SF49RIBONUCLEASE 3coord: 20..222
IPR033130Ribonuclease T2, His active site 2PROSITEPS00531RNASE_T2_2coord: 116..127
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 38..227
e-value: 7.27225E-63
score: 192.547

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12723.T1Cucsat.G12723.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006401 RNA catabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0005576 extracellular region
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding