Cucsat.G12684 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12684
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionribosomal RNA small subunit methyltransferase NEP1
Locationctg1838: 2378760 .. 2381343 (-)
RNA-Seq ExpressionCucsat.G12684
SyntenyCucsat.G12684
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGACGATAGAGCCCAGCCCATAAATCTGCTAATGCAAAAGGTTTCAACTACTCTACAATCAATCTATTCCAAGGGTTTTAGCACTAAGCTTCGACTACCGTCAAGGAAGTTCCTGCTCGGACGAACCATGGTTCGACCATTTGCGGCGAAGGGGAAGAAGAGAAAGAAGTCGGAGAAATACGACAGAGACGAAGATGCCGAAGAATCCACCTCTCCGTCCAAGAAAGTTATGTTGGAGAACGAACCTGATGAAGAACCAGCCAAGGAGGACTTTCATGAACTTGAAGGTATTCCTATTGCTCCGAAAGACCCCAAGAATGATAGCAATGCTGGTGTTATCTTCATCCTTGAAAGAGCTTCTCTGGAAGTTGCCAAAGTTGGGAAGGTAATGTATTGTTTTTCACTTACTGCTTTGAAGTTTTAGGATTTCCTTCATTTTGGGCGTTTAACAAAGTTATTTCATGCTTTTTATGAATTTTAAGAAGCTAATTATTGATTTTATATTCTTGGATTTCCATGTTTCGTGCTTATAGAATTACCAGCTGTTGAATTCTGATGACCACTCCAATTATTTGCGGAGGAATAACAGAAATCCTGGGGATTATAGACCTGATATCTTGCATCAAGTAAGTTGTTGTAGCTCATTGTTGTTTTTTCCCCAATGAAATAGGGAATTGTTTTATGGAATGAAAAATATAATTTTGTATGTTGATTATTTGGGTTTCAGTAGTGATATTAAATGTAAATGATTCAGGCGCTTTTGGCGATTTTCGATAGCCGAATTGCAAAGGCTGGAAGATTGAAAGTTGTTTATGTGAAAACTGAGAAAGGTTTATTAATTGAAATTAAACCATACGTTCGTCTTCCAAGGACGCAGAAACGATTTTATGGAGTTATGTGTAAGTGGATTTGCTTGTTCTAATTTTGCTGGACATCTCTGTTATTTGAGTGTGTTGTAATCAATGCAATTTCCCCTTTTATAGACCTTTCCATTGATCTTTTGACAAATGCTTCGCAGTGCAATTGCTACAAAAGTTAAGCATCACAGCTGCGGGTAAGCGTGAGAAACTCTTTCGGGTGATAAAGAACCCTGTAACTCAGTACTTACCTGCCAATTGTCGTAAAATGGGTAAGCCAATGCATGTCTTCCTCTTTTGTGGGTTTTGGTAGAAACTTTTCATACTCTTTTCTTGCATACCAGGCTTCTCGCATAGTTCGGATAAGTTGGTCAAGGTGCGAAATTATCTGGATGCTGTCAAAGATGATGTAGACCTTGTTTTTGTGGTATCTTCAAACCTCCTAACTGAAAATTACACTTTTTAATGCTTCATTTTTAGATGTATAACGTATGACAACTTCCCTTTATTGCTATGTATTAGGTGGGTGCAATGGCTCATGGGAAAATTGAGACTGATTACACGGATGATCTATTAGCAAGTAAGTTCGTTTACTTTTGCGTGTTTATTCAAGTAATCACATGGGAGTAAATTACCACATAACTATATTTACCTTTAACTTGAAAGTTTTTGACACACTGCGCTCCAAATTACCCTGTCTAACCTATGTTAACCATGGGTTCCAACTACACTACGGAACTCATTGTCAAACTTAAAAACATAATAACACAACACTATTTTGGATATATTGTTGGTATGCCAGGTTAGTTTGGGGCGGAGTGTCTCAAAATTTTCAAGTACTTGTGATTTAGTTATTCATCTGCAGTATGAGATAGAAATTATCATTTAGAAAATGGGTATCAACAGATATTAGCTAGAAAAGGTCTGAGTAAGAGGAAAAAGTCTATAAAGTATCAACAGAGAGAAGTTCGGGGGGCAAAAGTCTATAAAGTAAGAGGAAAAGCAGTACGGTAGAAGGGTTTTAAAGTTCTAACTATTTAAAAAGCATTTCCCAAGATGTCATAGAGTGGATATTGCTTCATAATTGTAACATTTTTATTAGTTAGCAATTGCTCAAACTTCTACTGTTATTCTTGGGTAGTATAAATTTTGTATGAAGTTAAATCCATTGCCTTGGCCTTGGGAATCCTAAATTCTTCAATTTATGCTATATTTGTGCAGTTTCTGAATATCCTCTTAGTGCTAGTTGCTGTATTGCGGATATTTGCAAAGATTTGGCAGAGAAATGGAATGTAGGGTGAACTCTGCTTACAACTCCATCAAAATCAGTGCCTCAACTATCTTGTTTCATATGTTTAGTTATTATTTCAGAAAATATGATCCAGCAGAGTTTTTAGGTTTATTAGTGGAACCATAATAGATCAATATTCCATTTTTTAAATTAAAATATTAATACCTCTTCTAAATTTAATTGAAAGCATAGAACTTTTAGCTGTAAGAAGTGATTGTACTGAATTTTGTCTGAGTTCTTTGCTGTTATCACTTGTGAAGTAAAAGTTTCAACTTATTTATGTCCATTCAATAGGTTGAAATTGAAATCGAGGGTATGTAAGAATTGATCCACGAACTCAGTTTTTTTCCCCTTCCAATATTTGAGATTAGTTGTATTTCAATGGTAACAAAGTTTGATTTCAACTTGAAACTTACTTTGAATCACTCATGGCCT

Coding sequence (CDS)

GACGATAGAGCCCAGCCCATAAATCTGCTAATGCAAAAGGTTTCAACTACTCTACAATCAATCTATTCCAAGGGTTTTAGCACTAAGCTTCGACTACCGTCAAGGAAGTTCCTGCTCGGACGAACCATGGTTCGACCATTTGCGGCGAAGGGGAAGAAGAGAAAGAAGTCGGAGAAATACGACAGAGACGAAGATGCCGAAGAATCCACCTCTCCGTCCAAGAAAGTTATGTTGGAGAACGAACCTGATGAAGAACCAGCCAAGGAGGACTTTCATGAACTTGAAGGTATTCCTATTGCTCCGAAAGACCCCAAGAATGATAGCAATGCTGGTGTTATCTTCATCCTTGAAAGAGCTTCTCTGGAAGTTGCCAAAGTTGGGAAGAATTACCAGCTGTTGAATTCTGATGACCACTCCAATTATTTGCGGAGGAATAACAGAAATCCTGGGGATTATAGACCTGATATCTTGCATCAAGCGCTTTTGGCGATTTTCGATAGCCGAATTGCAAAGGCTGGAAGATTGAAAGTTGTTTATGTGAAAACTGAGAAAGGTTTATTAATTGAAATTAAACCATACGTTCGTCTTCCAAGGACGCAGAAACGATTTTATGGAGTTATGTTGCAATTGCTACAAAAGTTAAGCATCACAGCTGCGGGTAAGCGTGAGAAACTCTTTCGGGTGATAAAGAACCCTGTAACTCAGTACTTACCTGCCAATTGTCGTAAAATGGGCTTCTCGCATAGTTCGGATAAGTTGGTCAAGGTGCGAAATTATCTGGATGCTGTCAAAGATGATGTAGACCTTGTTTTTGTGGTGGGTGCAATGGCTCATGGGAAAATTGAGACTGATTACACGGATGATCTATTAGCAATTTCTGAATATCCTCTTAGTGCTAGTTGCTGTATTGCGGATATTTGCAAAGATTTGGCAGAGAAATGGAATGTAGGGTGA

Protein sequence

DDRAQPINLLMQKVSTTLQSIYSKGFSTKLRLPSRKFLLGRTMVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEPAKEDFHELEGIPIAPKDPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG
Homology
BLAST of Cucsat.G12684 vs. ExPASy Swiss-Prot
Match: O35130 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Mus musculus OX=10090 GN=Emg1 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-47
Identity = 98/205 (47.80%), Postives = 136/205 (66.34%), Query Frame = 0

Query: 112 VIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAK 171
           +I +LE ASLE  KVGK Y+LLN D H + L +N R+PG+ RPDI HQ+LL + DS + +
Sbjct: 43  LIVVLEGASLETVKVGKTYELLNCDRHKSMLLKNGRDPGEVRPDITHQSLLMLMDSPLNR 102

Query: 172 AGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKN 231
           AG L+ VY+ T+K +LIE+ P  R+PRT  RF G+M+QLL KLS+ AA   +KL +VIKN
Sbjct: 103 AGLLQ-VYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKN 162

Query: 232 PVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLA 291
           PV+ + P  C K+G S S + +  +R   + V     +VFVVGA AHGK+  +YT+ +++
Sbjct: 163 PVSDHFPVGCMKIGTSFSVEDISDIR---ELVPSSDPVVFVVGAFAHGKVSVEYTEKMVS 222

Query: 292 ISEYPLSASCCIADICKDLAEKWNV 317
           IS YPLSA+   A +     E W V
Sbjct: 223 ISNYPLSAALTCAKVTTAFEEVWGV 243

BLAST of Cucsat.G12684 vs. ExPASy Swiss-Prot
Match: Q92979 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Homo sapiens OX=9606 GN=EMG1 PE=1 SV=4)

HSP 1 Score: 189.5 bits (480), Expect = 5.8e-47
Identity = 106/241 (43.98%), Postives = 148/241 (61.41%), Query Frame = 0

Query: 81  EPDEEPAKEDFHELEGIPIAPKDPKNDSNAGV-----IFILERASLEVAKVGKNYQLLNS 140
           +P E    E   + + +P  PK P+  +   +     I +LE ASLE  KVGK Y+LLN 
Sbjct: 9   KPRERSGGEQAQDWDALP--PKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNC 68

Query: 141 DDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVR 200
           D H + L +N R+PG+ RPDI HQ+LL + DS + +AG L+ VY+ T+K +LIE+ P  R
Sbjct: 69  DKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQ-VYIHTQKNVLIEVNPQTR 128

Query: 201 LPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVK 260
           +PRT  RF G+M+QLL KLS+ AA   +KL +VIKNPV+ + P  C K+G S S   +  
Sbjct: 129 IPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSD 188

Query: 261 VRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWN 317
           VR   + V     +VFVVGA AHGK+  +YT+ +++IS YPLSA+   A +     E W 
Sbjct: 189 VR---ELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWG 243

BLAST of Cucsat.G12684 vs. ExPASy Swiss-Prot
Match: Q9W4J5 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Drosophila melanogaster OX=7227 GN=CG3527 PE=3 SV=2)

HSP 1 Score: 188.0 bits (476), Expect = 1.7e-46
Identity = 97/216 (44.91%), Postives = 133/216 (61.57%), Query Frame = 0

Query: 106 NDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIF 165
           N +   +I +LE A LE  KV   ++LLN DDH+  +R+N R+PG  RPDI HQ LL +F
Sbjct: 37  NATEKRLIIVLEGAQLETVKVHNTFELLNCDDHAGIMRKNQRDPGSCRPDITHQCLLMLF 96

Query: 166 DSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKL 225
           DS + +AG L+ V+V+TE  +LIEI P  R+PRT KRF G+M+QLL K  I A     +L
Sbjct: 97  DSPLNRAGLLQ-VFVRTEHNVLIEINPQTRIPRTFKRFAGLMVQLLHKFQIRANDSSRRL 156

Query: 226 FRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYL-----DAVKDDVDLVFVVGAMAHGK 285
             VIKNP+T ++P  C+K   S S   L   R+ +      +   D  +V V+GA AHG 
Sbjct: 157 MSVIKNPITDHVPVGCKKYAMSFSGKLLPNCRDLVPHGDETSASYDEPVVIVIGAFAHGV 216

Query: 286 IETDYTDDLLAISEYPLSASCCIADICKDLAEKWNV 317
           ++TDYT++L +IS YPLSA+   + IC    E W V
Sbjct: 217 LKTDYTEELFSISNYPLSAAIACSKICSAFEEVWGV 251

BLAST of Cucsat.G12684 vs. ExPASy Swiss-Prot
Match: Q06287 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EMG1 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.2e-45
Identity = 99/230 (43.04%), Postives = 141/230 (61.30%), Query Frame = 0

Query: 97  IPIAPK--DPKNDSNAGVIFILERASLEVAKVGKN------YQLLNSDDHSNYLRRNNRN 156
           +P AP     K+     +I +L  ASLE  K+  N      Y LLN DDH   L++  R+
Sbjct: 24  VPQAPPVLTSKDKITKRMIVVLAMASLETHKISSNGPGGDKYVLLNCDDHQGLLKKMGRD 83

Query: 157 PGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVML 216
             + RPDI HQ LL + DS I KAG+L+ VY++T +G+LIE+ P VR+PRT KRF G+M+
Sbjct: 84  ISEARPDITHQCLLTLLDSPINKAGKLQ-VYIQTSRGILIEVNPTVRIPRTFKRFSGLMV 143

Query: 217 QLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVD 276
           QLL KLSI +    EKL +VIKNP+T +LP  CRK+  S  +  +++V++Y++ + DD  
Sbjct: 144 QLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKVTLSFDA-PVIRVQDYIEKLDDDES 203

Query: 277 LVFVVGAMAHGK--IETDYTDDLLAISEYPLSASCCIADICKDLAEKWNV 317
           +   VGAMA GK     +Y D+ + +S YPLSAS   +  C    + WN+
Sbjct: 204 ICVFVGAMARGKDNFADEYVDEKVGLSNYPLSASVACSKFCHGAEDAWNI 251

BLAST of Cucsat.G12684 vs. ExPASy Swiss-Prot
Match: Q9XX15 (Ribosomal RNA small subunit methyltransferase nep-1 OS=Caenorhabditis elegans OX=6239 GN=Y39A1A.14 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.1e-45
Identity = 95/202 (47.03%), Postives = 131/202 (64.85%), Query Frame = 0

Query: 115 ILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGR 174
           +LE  SLE AKVG  Y +L+SD H+N+LR+  ++P DYRPDILHQ LL + DS + +AG+
Sbjct: 31  VLEGCSLETAKVGGEYAILSSDKHANFLRKQKKDPADYRPDILHQCLLNLLDSPLNRAGK 90

Query: 175 LKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVT 234
           L+ V+ +T K +L+++ P  R+PRT  RF G+M+QLL KLSI AA   +KL  V+KNPV+
Sbjct: 91  LR-VFFRTSKNVLVDVSPQCRIPRTFDRFCGLMVQLLHKLSIRAAETTQKLMSVVKNPVS 150

Query: 235 QYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISE 294
            +LP   RKM  S +  +L  + N L A + D  LV ++G +A GKI  DY D    IS 
Sbjct: 151 NHLPVGSRKMLMSFNVPELT-MANKLVAPETDEPLVLIIGGIARGKIVVDYNDSETKISN 210

Query: 295 YPLSASCCIADICKDLAEKWNV 317
           YPLSA+   A +   L E W +
Sbjct: 211 YPLSAALTCAKVTSGLEEIWGI 230

BLAST of Cucsat.G12684 vs. NCBI nr
Match: XP_004136640.2 (ribosomal RNA small subunit methyltransferase nep-1 [Cucumis sativus] >KAE8651185.1 hypothetical protein Csa_001834 [Cucumis sativus])

HSP 1 Score: 537 bits (1383), Expect = 1.69e-191
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEPAKEDFHELEGIPIAPK 102
           MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEPAKEDFHELEGIPIAPK
Sbjct: 1   MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEPAKEDFHELEGIPIAPK 60

Query: 103 DPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 162
           DPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL
Sbjct: 61  DPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 120

Query: 163 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 222
           AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR
Sbjct: 121 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 180

Query: 223 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIE 282
           EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIE
Sbjct: 181 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIE 240

Query: 283 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG
Sbjct: 241 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 275

BLAST of Cucsat.G12684 vs. NCBI nr
Match: XP_008443268.1 (PREDICTED: ribosomal RNA small subunit methyltransferase NEP1 [Cucumis melo] >KAA0053876.1 ribosomal RNA small subunit methyltransferase NEP1 [Cucumis melo var. makuwa] >TYK25528.1 ribosomal RNA small subunit methyltransferase NEP1 [Cucumis melo var. makuwa])

HSP 1 Score: 522 bits (1345), Expect = 1.04e-185
Identity = 265/275 (96.36%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEPAKEDFHELEGIPIAPK 102
           MVRPFAAKGKKRKK+EKYDRDED EES+SP+KKVM+ENEPDEEP KEDFHELEGIPI PK
Sbjct: 1   MVRPFAAKGKKRKKAEKYDRDEDGEESSSPAKKVMVENEPDEEPPKEDFHELEGIPIVPK 60

Query: 103 DPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 162
           DPK D+NAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL
Sbjct: 61  DPKTDNNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 120

Query: 163 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 222
           AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR
Sbjct: 121 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 180

Query: 223 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIE 282
           EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVK+RNYLDAVKDDVDLVFVVGAMAHGKIE
Sbjct: 181 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKMRNYLDAVKDDVDLVFVVGAMAHGKIE 240

Query: 283 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG
Sbjct: 241 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 275

BLAST of Cucsat.G12684 vs. NCBI nr
Match: XP_038905075.1 (ribosomal RNA small subunit methyltransferase nep-1 [Benincasa hispida])

HSP 1 Score: 498 bits (1282), Expect = 4.13e-176
Identity = 252/275 (91.64%), Postives = 263/275 (95.64%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEPAKEDFHELEGIPIAPK 102
           MVRPFAAKGKKRKKSEKYDRD+D  EST P+KK   ENEPDEEP KEDFHELEGIPI PK
Sbjct: 1   MVRPFAAKGKKRKKSEKYDRDDDDGESTVPAKKPTAENEPDEEPPKEDFHELEGIPIVPK 60

Query: 103 DPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 162
           DP +D +AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LR+NNRNPGDYRPDILHQALL
Sbjct: 61  DPNSDKHAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRKNNRNPGDYRPDILHQALL 120

Query: 163 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 222
           AIFDSRIAKAGRLK VYVKTEKGLLIE+KPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR
Sbjct: 121 AIFDSRIAKAGRLKAVYVKTEKGLLIEVKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 180

Query: 223 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIE 282
           EKLFRVIKNPVTQYLPAN RKMGFSHSSDKLVK+RNYLDAV+DDVDLVFVVGAMAHGKIE
Sbjct: 181 EKLFRVIKNPVTQYLPANSRKMGFSHSSDKLVKMRNYLDAVEDDVDLVFVVGAMAHGKIE 240

Query: 283 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           TDYTDDLLAISEYPLSASCCIADICKDLAEKWN+G
Sbjct: 241 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNIG 275

BLAST of Cucsat.G12684 vs. NCBI nr
Match: KAG7017643.1 (Ribosomal RNA small subunit methyltransferase NEP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 493 bits (1269), Expect = 4.57e-174
Identity = 252/279 (90.32%), Postives = 267/279 (95.70%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEP----AKEDFHELEGIP 102
           MVRPFAAKGKKRKK+E+YDRD+D EEST+ +KK M+ENEPDE+P    A+E+FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 103 IAPKDPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILH 162
           I PKDPK+DS AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 163 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 222
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 223 AGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAH 282
           AGKREKLFRVIKNPVTQYLP+N RKMGFSHSSDKLVKVR YLDAV+DDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 283 GKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           GKIET YTDDLLAISEYPLSASCCIADICKDLA+KWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

BLAST of Cucsat.G12684 vs. NCBI nr
Match: XP_022935622.1 (ribosomal RNA small subunit methyltransferase nep-1 [Cucurbita moschata] >KAG6580885.1 Ribosomal RNA small subunit methyltransferase NEP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 493 bits (1269), Expect = 4.57e-174
Identity = 252/279 (90.32%), Postives = 267/279 (95.70%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEP----AKEDFHELEGIP 102
           MVRPFAAKGKKRKK+E+YDRD+D EEST+ +KK M+ENEPDE+P    A+E+FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 103 IAPKDPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILH 162
           I PKDPK+DS AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 163 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 222
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 223 AGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAH 282
           AGKREKLFRVIKNPVTQYLP+N RKMGFSHSSDKLVKVR YLDAV+DDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 283 GKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           GKIET YTDDLLAISEYPLSASCCIADICKDLA+KWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

BLAST of Cucsat.G12684 vs. ExPASy TrEMBL
Match: A0A0A0LFI3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G821550 PE=3 SV=1)

HSP 1 Score: 596 bits (1537), Expect = 9.05e-215
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 11  MQKVSTTLQSIYSKGFSTKLRLPSRKFLLGRTMVRPFAAKGKKRKKSEKYDRDEDAEEST 70
           MQKVSTTLQSIYSKGFSTKLRLPSRKFLLGRTMVRPFAAKGKKRKKSEKYDRDEDAEEST
Sbjct: 1   MQKVSTTLQSIYSKGFSTKLRLPSRKFLLGRTMVRPFAAKGKKRKKSEKYDRDEDAEEST 60

Query: 71  SPSKKVMLENEPDEEPAKEDFHELEGIPIAPKDPKNDSNAGVIFILERASLEVAKVGKNY 130
           SPSKKVMLENEPDEEPAKEDFHELEGIPIAPKDPKNDSNAGVIFILERASLEVAKVGKNY
Sbjct: 61  SPSKKVMLENEPDEEPAKEDFHELEGIPIAPKDPKNDSNAGVIFILERASLEVAKVGKNY 120

Query: 131 QLLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEI 190
           QLLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEI
Sbjct: 121 QLLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEI 180

Query: 191 KPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSS 250
           KPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSS
Sbjct: 181 KPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSS 240

Query: 251 DKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISEYPLSASCCIADICKDL 310
           DKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISEYPLSASCCIADICKDL
Sbjct: 241 DKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISEYPLSASCCIADICKDL 300

Query: 311 AEKWNVG 317
           AEKWNVG
Sbjct: 301 AEKWNVG 307

BLAST of Cucsat.G12684 vs. ExPASy TrEMBL
Match: A0A5D3DPQ6 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006800 PE=3 SV=1)

HSP 1 Score: 522 bits (1345), Expect = 5.05e-186
Identity = 265/275 (96.36%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEPAKEDFHELEGIPIAPK 102
           MVRPFAAKGKKRKK+EKYDRDED EES+SP+KKVM+ENEPDEEP KEDFHELEGIPI PK
Sbjct: 1   MVRPFAAKGKKRKKAEKYDRDEDGEESSSPAKKVMVENEPDEEPPKEDFHELEGIPIVPK 60

Query: 103 DPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 162
           DPK D+NAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL
Sbjct: 61  DPKTDNNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 120

Query: 163 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 222
           AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR
Sbjct: 121 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 180

Query: 223 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIE 282
           EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVK+RNYLDAVKDDVDLVFVVGAMAHGKIE
Sbjct: 181 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKMRNYLDAVKDDVDLVFVVGAMAHGKIE 240

Query: 283 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG
Sbjct: 241 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 275

BLAST of Cucsat.G12684 vs. ExPASy TrEMBL
Match: A0A1S3B7L0 (ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo OX=3656 GN=LOC103486891 PE=3 SV=1)

HSP 1 Score: 522 bits (1345), Expect = 5.05e-186
Identity = 265/275 (96.36%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEPAKEDFHELEGIPIAPK 102
           MVRPFAAKGKKRKK+EKYDRDED EES+SP+KKVM+ENEPDEEP KEDFHELEGIPI PK
Sbjct: 1   MVRPFAAKGKKRKKAEKYDRDEDGEESSSPAKKVMVENEPDEEPPKEDFHELEGIPIVPK 60

Query: 103 DPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 162
           DPK D+NAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL
Sbjct: 61  DPKTDNNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILHQALL 120

Query: 163 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 222
           AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR
Sbjct: 121 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 180

Query: 223 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAHGKIE 282
           EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVK+RNYLDAVKDDVDLVFVVGAMAHGKIE
Sbjct: 181 EKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKMRNYLDAVKDDVDLVFVVGAMAHGKIE 240

Query: 283 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG
Sbjct: 241 TDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 275

BLAST of Cucsat.G12684 vs. ExPASy TrEMBL
Match: A0A6J1F634 (ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita moschata OX=3662 GN=LOC111442430 PE=3 SV=1)

HSP 1 Score: 493 bits (1269), Expect = 2.21e-174
Identity = 252/279 (90.32%), Postives = 267/279 (95.70%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEP----AKEDFHELEGIP 102
           MVRPFAAKGKKRKK+E+YDRD+D EEST+ +KK M+ENEPDE+P    A+E+FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 103 IAPKDPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILH 162
           I PKDPK+DS AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 163 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 222
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 223 AGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAH 282
           AGKREKLFRVIKNPVTQYLP+N RKMGFSHSSDKLVKVR YLDAV+DDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 283 GKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           GKIET YTDDLLAISEYPLSASCCIADICKDLA+KWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

BLAST of Cucsat.G12684 vs. ExPASy TrEMBL
Match: A0A6J1J0G6 (ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita maxima OX=3661 GN=LOC111482341 PE=3 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 1.81e-173
Identity = 251/279 (89.96%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 43  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEP----AKEDFHELEGIP 102
           MVRPFAAKGKKRKK+E+YDRD+D EES + +KK M+ENEPDE+P    A+E+FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKTERYDRDDDGEESIASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 103 IAPKDPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILH 162
           I PKDPK+DS AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 163 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 222
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 223 AGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAH 282
           AGKREKLFRVIKNPVTQYLP+N RKMGFSHSSDKLVKVR YLDAV+DDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 283 GKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 317
           GKIET YTDDLLAISEYPLSASCCIADICKDLA+KWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O351301.2e-4747.80Ribosomal RNA small subunit methyltransferase NEP1 OS=Mus musculus OX=10090 GN=E... [more]
Q929795.8e-4743.98Ribosomal RNA small subunit methyltransferase NEP1 OS=Homo sapiens OX=9606 GN=EM... [more]
Q9W4J51.7e-4644.91Ribosomal RNA small subunit methyltransferase NEP1 OS=Drosophila melanogaster OX... [more]
Q062874.2e-4543.04Ribosomal RNA small subunit methyltransferase NEP1 OS=Saccharomyces cerevisiae (... [more]
Q9XX157.1e-4547.03Ribosomal RNA small subunit methyltransferase nep-1 OS=Caenorhabditis elegans OX... [more]
Match NameE-valueIdentityDescription
XP_004136640.21.69e-191100.00ribosomal RNA small subunit methyltransferase nep-1 [Cucumis sativus] >KAE865118... [more]
XP_008443268.11.04e-18596.36PREDICTED: ribosomal RNA small subunit methyltransferase NEP1 [Cucumis melo] >KA... [more]
XP_038905075.14.13e-17691.64ribosomal RNA small subunit methyltransferase nep-1 [Benincasa hispida][more]
KAG7017643.14.57e-17490.32Ribosomal RNA small subunit methyltransferase NEP1, partial [Cucurbita argyrospe... [more]
XP_022935622.14.57e-17490.32ribosomal RNA small subunit methyltransferase nep-1 [Cucurbita moschata] >KAG658... [more]
Match NameE-valueIdentityDescription
A0A0A0LFI39.05e-215100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G821550 PE=3 SV=1[more]
A0A5D3DPQ65.05e-18696.36Ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo var. makuwa O... [more]
A0A1S3B7L05.05e-18696.36ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1F6342.21e-17490.32ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita moschata OX=366... [more]
A0A6J1J0G61.81e-17389.96ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita maxima OX=3661 ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005304Ribosomal biogenesis, methyltransferase, EMG1/NEP1PFAMPF03587EMG1coord: 90..265
e-value: 5.2E-59
score: 199.3
IPR005304Ribosomal biogenesis, methyltransferase, EMG1/NEP1PANTHERPTHR12636NEP1/MRA1coord: 26..265
IPR005304Ribosomal biogenesis, methyltransferase, EMG1/NEP1CDDcd18088Nep1-likecoord: 88..267
e-value: 1.57157E-85
score: 252.836
IPR029026tRNA (guanine-N1-)-methyltransferase, N-terminalGENE3D3.40.1280.10coord: 88..273
e-value: 1.8E-71
score: 241.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..68
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..68
IPR029028Alpha/beta knot methyltransferasesSUPERFAMILY75217alpha/beta knotcoord: 80..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12684.T1Cucsat.G12684.T1mRNA
Cucsat.G12684.T2Cucsat.G12684.T2mRNA
Cucsat.G12684.T3Cucsat.G12684.T3mRNA
Cucsat.G12684.T4Cucsat.G12684.T4mRNA
Cucsat.G12684.T5Cucsat.G12684.T5mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070475 rRNA base methylation
cellular_component GO:0005730 nucleolus
cellular_component GO:0032040 small-subunit processome
molecular_function GO:0019843 rRNA binding
molecular_function GO:0070037 rRNA (pseudouridine) methyltransferase activity