Cucsat.G12332 (gene) Cucumber (B10) v3

BLAST of Cucsat.G12332 vs. ExPASy Swiss-Prot
Match: Q9SHY2 (Probable metal-nicotianamine transporter YSL7 OS=Arabidopsis thaliana OX=3702 GN=YSL7 PE=2 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 1.8e-255
Identity = 449/691 (64.98%), Postives = 548/691 (79.31%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFK--NLEVPSWRNQITFRALFTSFILSIVF 60
           ME   SKK+++  +GS ++   E I VE  F+  N   P W+ Q+TFRAL  SFIL+I+F
Sbjct: 1   MEVERSKKDDDLNNGSKSN-EEEEISVERIFEESNEIPPPWQKQLTFRALIVSFILAILF 60

Query: 61  NFIVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVA 120
            F+V KLNLTTG+IPSLN++AGLLGF  +K +T IL++ G +KQPFTRQENTVIQTCVVA
Sbjct: 61  TFVVMKLNLTTGIIPSLNISAGLLGFFFVKSWTKILNKAGFLKQPFTRQENTVIQTCVVA 120

Query: 121 SSGIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPL 180
           SSGIAFS G  SYL GMS  +A Q+ E N P+NIK   +GWMIGFLFVVSF+GLFS+VPL
Sbjct: 121 SSGIAFSGGFGSYLFGMSDVVAKQSAEANTPLNIKNPHLGWMIGFLFVVSFLGLFSVVPL 180

Query: 181 RKMMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAA 240
           RK+MI+ +KLTYPSGTATA+LINSFHTP+GAKLAKKQV  L K F FSF++  FQWFFA 
Sbjct: 181 RKIMIVDFKLTYPSGTATAHLINSFHTPQGAKLAKKQVRALGKFFSFSFLWGFFQWFFAT 240

Query: 241 ADGCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIE 300
            DGCGF++FPTFGL+AY  +FYFDFS+TYVGVGMICP+++N+SLL+GAI+SWG+MWPLI 
Sbjct: 241 GDGCGFANFPTFGLKAYENKFYFDFSATYVGVGMICPYLINVSLLIGAILSWGVMWPLIG 300

Query: 301 LRKGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGS--- 360
            +KG WY A LS++SLHG+QGYRVFIAIAM+LGDGLY+   +L +T + L KQ       
Sbjct: 301 AQKGKWYAADLSSTSLHGLQGYRVFIAIAMILGDGLYNFIKVLGRTVFGLYKQFKNKDVL 360

Query: 361 --GNADSSLEIT-DYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYH 420
              +  S+  +T  YD +RR E FLKD+IP+W A+ GYV+LA +SII VP IFHQLKWYH
Sbjct: 361 PINDHTSTAPVTISYDDKRRTELFLKDRIPSWFAVTGYVVLAIVSIITVPHIFHQLKWYH 420

Query: 421 VLVAYVIAPVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMM 480
           +L+ Y+IAPVLAFCNAYGCGLTDWSLAS YGK AI    +W G  NGGV+AGLA+CGVMM
Sbjct: 421 ILIMYIIAPVLAFCNAYGCGLTDWSLASTYGKLAIFTIGAWAGASNGGVLAGLAACGVMM 480

Query: 481 SIVSTASDLMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAY-NIGDPEG 540
           +IVSTASDLMQDFKTGY+TLASPRSMF SQ  GTAMGCV+SP VFW F+KA+ + G P  
Sbjct: 481 NIVSTASDLMQDFKTGYMTLASPRSMFLSQAIGTAMGCVISPCVFWLFYKAFPDFGQPGT 540

Query: 541 SYPAPYGLMYRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYR 600
           +YPAPY L+YR +++LGVEG S+LPK+CL L   FF  A+++N IRD L       +  R
Sbjct: 541 AYPAPYALVYRNMSILGVEGFSALPKHCLMLCYIFFAAAVIVNGIRDALGP-----KWAR 600

Query: 601 FIPSPMCMAIPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSV 660
           FIP PM MAIPFYLG YF IDMC+GSLILFIW++ NK KA  ++ AVASGLICGE +W++
Sbjct: 601 FIPLPMAMAIPFYLGGYFTIDMCLGSLILFIWRKLNKPKADAYSSAVASGLICGEGIWTL 660

Query: 661 PAAILALAGVKAPLCMKFLSSSTNAKVDAFL 683
           P++ILALAGVKAP+CMKFLS ++N KVDAFL
Sbjct: 661 PSSILALAGVKAPICMKFLSMASNNKVDAFL 685

BLAST of Cucsat.G12332 vs. ExPASy Swiss-Prot
Match: Q5JQD7 (Probable metal-nicotianamine transporter YSL12 OS=Oryza sativa subsp. japonica OX=39947 GN=YSL12 PE=2 SV=2)

HSP 1 Score: 845.5 bits (2183), Expect = 4.2e-244
Identity = 433/665 (65.11%), Postives = 518/665 (77.89%), Query Frame = 0

Query: 27  VEDAFKNLEVPSWRNQITFRALFTSFILSIVFNFIVCKLNLTTGVIPSLNVAAGLLGFAI 86
           VE AF +  VPSWR Q+T RA   SF LSI+F+ IV KLNLTTG+IPSLNV+AGLLGF  
Sbjct: 56  VERAFVDRAVPSWREQLTVRAFVVSFFLSIMFSIIVMKLNLTTGIIPSLNVSAGLLGFFF 115

Query: 87  LKGYTSILDQLGLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQAEEG 146
           ++ +T+ ++++GL++QPFTRQENTVIQTCVVA+ GIAFS G  +YL GMS  IA QA E 
Sbjct: 116 VRLWTAAIERVGLLRQPFTRQENTVIQTCVVAAYGIAFSGGFGTYLFGMSETIAKQATEA 175

Query: 147 NMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRKMMIIKYKLTYPSGTATAYLINSFHTP 206
           N   N+K   +GWMIGFLF+VSF+GL ++VPLRK+MI+ YKLTYPSGTATAYLIN FHTP
Sbjct: 176 NNAQNVKNPHIGWMIGFLFLVSFIGLLALVPLRKIMIVDYKLTYPSGTATAYLINGFHTP 235

Query: 207 KGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAADGCGFSSFPTFGLQAYAKRFYFDFSST 266
           +GAKLAKKQV  L K F FSFV+  FQWF+ A DGCGF SFPT GLQAY  RFYFDFS T
Sbjct: 236 EGAKLAKKQVKELGKFFLFSFVWGFFQWFYTAGDGCGFQSFPTLGLQAYKNRFYFDFSPT 295

Query: 267 YVGVGMICPFMVNLSLLLGAIISWGIMWPLIELRKGDWYNASLSASSLHGIQGYRVFIAI 326
           YVGVGMICP +VN+S+LLG I+SWGIMWPLI  +KG WY ASLS +SLHG+QGYRVFI+I
Sbjct: 296 YVGVGMICPHIVNVSVLLGGILSWGIMWPLIRNKKGSWYAASLSETSLHGLQGYRVFISI 355

Query: 327 AMMLGDGLYHVCFMLFQT---FYSLSKQK-----SGSGNADSSLEITDYDARRRMEYFLK 386
           A++LGDGLY+   +L +T   F  + K+      S +G+   + E   +D  RR E FLK
Sbjct: 356 ALILGDGLYNFVKVLIRTTAGFVVMMKKNSTLPVSNNGSPMVATEAISFDDERRTELFLK 415

Query: 387 DQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIAPVLAFCNAYGCGLTDWSL 446
           DQIP  VA  GYV +AA+SI  +P IF QLKWY++LVAYV APVLAFCNAYG GLTDWSL
Sbjct: 416 DQIPKTVAFGGYVAVAAVSIGTLPQIFPQLKWYYILVAYVFAPVLAFCNAYGAGLTDWSL 475

Query: 447 ASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLTLASPRSM 506
           AS YGK AI IF +W G  NGGV+ GLA+CGVMMSIVSTASDLMQDFKTGYLTLASPRSM
Sbjct: 476 ASTYGKLAIFIFGAWAGASNGGVLVGLAACGVMMSIVSTASDLMQDFKTGYLTLASPRSM 535

Query: 507 FFSQVAGTAMGCVLSPLVFWFFFKAY-NIGDPEGSYPAPYGLMYRGIALLGVEGVSSLPK 566
           F SQV GTAMGCV++P VFW F+KA+ +IG     YPAPY ++YR +A+LGV+G SSLPK
Sbjct: 536 FVSQVIGTAMGCVIAPCVFWLFYKAFADIGVSGTEYPAPYAIVYRNMAILGVDGFSSLPK 595

Query: 567 NCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMAIPFYLGAYFAIDMCVGS 626
           +CLTL   FF  AI IN+ RD+        ++ RFIP PM MAIPFY+G+YFAIDM +G+
Sbjct: 596 HCLTLCYIFFAAAIAINLARDL-----APSKVARFIPLPMAMAIPFYIGSYFAIDMFIGT 655

Query: 627 LILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAGVKAPLCMKFLSSSTNAK 683
           +ILF+W+  NK KA  FAPAVASGLICG+ +W++P +ILALA VK P+CMKFLS S NA+
Sbjct: 656 VILFVWEMVNKAKAEAFAPAVASGLICGDGIWTLPQSILALAKVKPPICMKFLSRSVNAQ 715

BLAST of Cucsat.G12332 vs. ExPASy Swiss-Prot
Match: Q6H7J6 (Probable metal-nicotianamine transporter YSL14 OS=Oryza sativa subsp. japonica OX=39947 GN=YSL14 PE=2 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 5.1e-242
Identity = 429/696 (61.64%), Postives = 531/696 (76.29%), Query Frame = 0

Query: 3   RNSSKKENEGESGSGTDGGG---ERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFN 62
           + + + E  G  G G +GG    +   VE  F +  VPSWR Q+T RA   S +L+++F+
Sbjct: 37  KGAEEHEAAGGGGGGRNGGADDPDATSVERVFADKAVPSWREQLTLRAFVVSALLAVMFS 96

Query: 63  FIVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVAS 122
            IV KLNLTTG+IPSLNV+AGLLGF  ++ +TS ++++GL+KQPFTRQENTVIQTCVV++
Sbjct: 97  VIVMKLNLTTGIIPSLNVSAGLLGFFFVRLWTSAVERIGLLKQPFTRQENTVIQTCVVSA 156

Query: 123 SGIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLR 182
            GIAFS G  SYL GMS  IA QA E     NIK   +GWMIGFLF+VSF+GLF++VPLR
Sbjct: 157 YGIAFSGGFGSYLFGMSETIAKQATEAKDAQNIKDPHLGWMIGFLFLVSFIGLFALVPLR 216

Query: 183 KMMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAA 242
           K+MI+ YKLTYPSGTATAYLIN FHTP+GAKLAKKQV  L K F FSF +  FQWF+ A 
Sbjct: 217 KIMIVDYKLTYPSGTATAYLINGFHTPEGAKLAKKQVKTLGKYFLFSFFWGFFQWFYTAG 276

Query: 243 DGCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIEL 302
           D CGF +FPT GL+AY  RF+FDFS TYVGVGMICP++VN+S+LLG I+SWG+MWPLI  
Sbjct: 277 DDCGFKNFPTLGLEAYNNRFFFDFSPTYVGVGMICPYIVNVSVLLGGILSWGVMWPLIAK 336

Query: 303 RKGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQT---FYSLSKQK---- 362
           +KG WY A +S +SLHG+Q YRVFI+IA++LGDGLY+   +L +T   F S+ +      
Sbjct: 337 KKGSWYPADISDNSLHGLQAYRVFISIALILGDGLYNFLKVLIRTIAGFISMVQNNSKGM 396

Query: 363 ---SGSGNADSSLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLK 422
              S +G + S+ E   +D  RR E FLKDQIP  VA  GYV++AA+SI  +P IF QLK
Sbjct: 397 LPVSDNGMSMSTAEEVSFDDERRTEIFLKDQIPKSVAYGGYVVVAALSIGTLPEIFPQLK 456

Query: 423 WYHVLVAYVIAPVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCG 482
           WY++LVAY++APVLAFCNAYG GLTDWSLAS YGK AI +F +W GL +GGV+ GLA+CG
Sbjct: 457 WYYILVAYIVAPVLAFCNAYGSGLTDWSLASTYGKLAIFVFGAWAGLSHGGVLVGLAACG 516

Query: 483 VMMSIVSTASDLMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAY-NIGD 542
           VMMSIVSTASDLMQDFKTGYLTLASPRSMF SQV GT MGCV++P VFW F+KA+ NIG 
Sbjct: 517 VMMSIVSTASDLMQDFKTGYLTLASPRSMFISQVIGTGMGCVIAPCVFWLFYKAFSNIGT 576

Query: 543 PEGSYPAPYGLMYRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFR 602
               YPAPY ++YR +A+LGV+G +SLP+NCLTL   FF  AI IN+IRD+        +
Sbjct: 577 SGTEYPAPYAIVYRNMAILGVDGFNSLPENCLTLCYIFFAAAIAINLIRDL-----APHK 636

Query: 603 IYRFIPSPMCMAIPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESL 662
           + RFIP PM MAIPFY+G+YFAIDM +GS+ILF+W++ NK KA  F PAVASGLICG+ +
Sbjct: 637 VSRFIPLPMAMAIPFYIGSYFAIDMFLGSVILFVWEKLNKAKADAFGPAVASGLICGDGI 696

Query: 663 WSVPAAILALAGVKAPLCMKFLSSSTNAKVDAFLEG 685
           W++P +ILALA VK P+CMKFLS + NAKVD+FL G
Sbjct: 697 WTLPQSILALAKVKPPICMKFLSRAANAKVDSFLAG 727

BLAST of Cucsat.G12332 vs. ExPASy Swiss-Prot
Match: Q9LUN2 (Probable metal-nicotianamine transporter YSL5 OS=Arabidopsis thaliana OX=3702 GN=YSL5 PE=1 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 3.7e-240
Identity = 419/673 (62.26%), Postives = 523/673 (77.71%), Query Frame = 0

Query: 27  VEDAFKNLEVPSWRNQITFRALFTSFILSIVFNFIVCKLNLTTGVIPSLNVAAGLLGFAI 86
           VE  F++ EVPSW+ Q+T RA   SF+LSI+F+FIV KLNLTTG+IPSLNV+AGLLGF  
Sbjct: 47  VEKIFESREVPSWKKQLTVRAFVVSFMLSILFSFIVMKLNLTTGIIPSLNVSAGLLGFFF 106

Query: 87  LKGYTSILDQLGLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQAEEG 146
           +K +T +L + GL+KQPFTRQENTVIQTCVVASSGIAFS G  +YL GMS +IA Q+  G
Sbjct: 107 VKTWTKMLHRSGLLKQPFTRQENTVIQTCVVASSGIAFSGGFGTYLFGMSERIATQS--G 166

Query: 147 NMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRKMMIIKYKLTYPSGTATAYLINSFHTP 206
           ++   +K  S+GW+IGFLFVVSF+GLFS+VPLRK+M+I +KLTYPSGTATA+LINSFHTP
Sbjct: 167 DVSRGVKDPSLGWIIGFLFVVSFLGLFSVVPLRKIMVIDFKLTYPSGTATAHLINSFHTP 226

Query: 207 KGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAADGCGFSSFPTFGLQAYAKRFYFDFSST 266
           +GAKLAKKQV VL K F  SF ++ FQWFF   + CGFS+FPTFGL+AY  +FYFDFS+T
Sbjct: 227 QGAKLAKKQVRVLGKFFSLSFFWSFFQWFFTGGENCGFSNFPTFGLKAYQYKFYFDFSAT 286

Query: 267 YVGVGMICPFMVNLSLLLGAIISWGIMWPLIELRKGDWYNASLSASSLHGIQGYRVFIAI 326
           YVGVGMICP+++N+S+LLG I+SWGIMWPLIE +KGDW+  ++ +SS+HG+Q Y+VFIA+
Sbjct: 287 YVGVGMICPYIINISVLLGGILSWGIMWPLIETKKGDWFPDNVPSSSMHGLQAYKVFIAV 346

Query: 327 AMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADSSLEIT--------------DYDARRR 386
           A++LGDGLY+ C +L +T   L  Q  G   + S    T               YD +RR
Sbjct: 347 AIILGDGLYNFCKVLSRTLSGLFVQLRGPTTSISRTSFTLEEDPHASPLSPKQSYDDQRR 406

Query: 387 MEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIAPVLAFCNAYGCG 446
             +FLKDQIP W A+ GY+ +AA S   +P +FHQL+WY++LV Y+ APVLAFCNAYG G
Sbjct: 407 TRFFLKDQIPTWFAVGGYITIAATSTAILPHMFHQLRWYYILVIYICAPVLAFCNAYGAG 466

Query: 447 LTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLTL 506
           LTDWSLAS YGK AI    +W G  +GG++AGLA+CGVMM+IVSTASDL QDFKTGYLTL
Sbjct: 467 LTDWSLASTYGKLAIFTIGAWAGSEHGGMLAGLAACGVMMNIVSTASDLTQDFKTGYLTL 526

Query: 507 ASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAY-NIGDPEGSYPAPYGLMYRGIALLGVEG 566
           +SP+SMF SQV GTAMGCV+SP VFW F+KA+ ++G P   YPAP+  +YR +A LGVEG
Sbjct: 527 SSPKSMFVSQVIGTAMGCVVSPCVFWLFYKAFDDLGLPNTEYPAPFATVYRSMAKLGVEG 586

Query: 567 VSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMAIPFYLGAYFAI 626
           V+SLP+ CL L   FF  AI++NI++D LH         RFIP PM MAIPF+LG YFAI
Sbjct: 587 VASLPRECLVLCYAFFGVAILVNIVKDSLHS-----NWGRFIPLPMAMAIPFFLGPYFAI 646

Query: 627 DMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAGVKAPLCMKFLS 685
           DMCVGSLILFIW+R +  KA  F  AVASGLICG+ +WS+P+++LA+AGV  P+CMKFLS
Sbjct: 647 DMCVGSLILFIWERVDAAKAEAFGTAVASGLICGDGIWSLPSSVLAIAGVNPPVCMKFLS 706

BLAST of Cucsat.G12332 vs. ExPASy Swiss-Prot
Match: Q6R3K4 (Probable metal-nicotianamine transporter YSL8 OS=Arabidopsis thaliana OX=3702 GN=YSL8 PE=1 SV=2)

HSP 1 Score: 828.9 bits (2140), Expect = 4.1e-239
Identity = 416/674 (61.72%), Postives = 526/674 (78.04%), Query Frame = 0

Query: 27  VEDAFKNLEVPSWRNQITFRALFTSFILSIVFNFIVCKLNLTTGVIPSLNVAAGLLGFAI 86
           VE  F++ EVPSW+ Q+T RA   SF LSI+F+FIV KLNLTTG+IPSLNV+AGLLGF  
Sbjct: 56  VEGIFESREVPSWKKQLTIRAFVVSFALSILFSFIVMKLNLTTGIIPSLNVSAGLLGFFF 115

Query: 87  LKGYTSILDQLGLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQAEEG 146
           +K +T +L + GL+KQPFTRQENTVIQTCVVASSGIAFS G  +YL  MS +IA Q+  G
Sbjct: 116 VKTWTKMLHKSGLLKQPFTRQENTVIQTCVVASSGIAFSGGFGTYLFAMSHRIADQS--G 175

Query: 147 NMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRKMMIIKYKLTYPSGTATAYLINSFHTP 206
           ++   +K  S+GWMI FLFVVSF+GLFS+VPLRK+MII +KL YPSGTATA+LINSFHTP
Sbjct: 176 DVARGVKDPSLGWMIAFLFVVSFLGLFSVVPLRKIMIIDFKLPYPSGTATAHLINSFHTP 235

Query: 207 KGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAADGCGFSSFPTFGLQAYAKRFYFDFSST 266
           +GAKLAKKQV VL K F FSF +  FQWFF A + CGF+SFPTFGL+AY  +FYFDFS+T
Sbjct: 236 QGAKLAKKQVRVLGKFFSFSFFWGFFQWFFTAGENCGFNSFPTFGLRAYQYKFYFDFSAT 295

Query: 267 YVGVGMICPFMVNLSLLLGAIISWGIMWPLIELRKGDWYNASLSASSLHGIQGYRVFIAI 326
           YVGVGMICP+++N+SLLLG I+SWG+MWPLIE RKGDW+ +++ +SS++G+Q Y+VFIA+
Sbjct: 296 YVGVGMICPYIINISLLLGGILSWGLMWPLIETRKGDWFPSNVDSSSMNGLQAYKVFIAV 355

Query: 327 AMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADSSLEITD---------------YDARR 386
           A +LGDGLY+ C +L +TF  L  Q  G   + SSL   +               YD +R
Sbjct: 356 ATILGDGLYNFCKVLIRTFSGLISQIRGKAGSRSSLAHKEDPPASPASPLTPRISYDDQR 415

Query: 387 RMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIAPVLAFCNAYGC 446
           R  +FLKDQIP+W A+ GYV+++A+S   +P +F QL+WY+++V Y+ AP+LAFCNAYG 
Sbjct: 416 RTRFFLKDQIPSWFAVGGYVVISAVSTAILPHMFSQLRWYYIIVIYIFAPILAFCNAYGA 475

Query: 447 GLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLT 506
           GLTDWSLAS YGK AI    +W G  +GG++AGLA+CGVMM+IVSTASDL QDFKTGYLT
Sbjct: 476 GLTDWSLASTYGKLAIFTIGAWAGSDHGGLLAGLAACGVMMNIVSTASDLTQDFKTGYLT 535

Query: 507 LASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAY-NIGDPEGSYPAPYGLMYRGIALLGVE 566
           L+SPR+MF SQV GTAMGC++SP VFW F+KA+ ++G P   YPAP+  +YR +A LGVE
Sbjct: 536 LSSPRAMFVSQVIGTAMGCLVSPCVFWLFYKAFDDLGLPNSEYPAPFATVYRSMAKLGVE 595

Query: 567 GVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMAIPFYLGAYFA 626
           GVSSLP++CL L   FF  AI+IN+I+D L       R  RF+P PM MAIPF+LG YFA
Sbjct: 596 GVSSLPRDCLMLCYVFFGVAILINLIKDCLGN-----RWGRFVPLPMAMAIPFFLGPYFA 655

Query: 627 IDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAGVKAPLCMKFL 685
           IDMCVGS ILF+W+R +  KA  FA AVASGLICG+ +W++P+++LA+AGVK P+CMKFL
Sbjct: 656 IDMCVGSFILFVWERLDAPKAEAFATAVASGLICGDGIWTLPSSVLAIAGVKPPICMKFL 715

BLAST of Cucsat.G12332 vs. NCBI nr
Match: XP_004148573.1 (probable metal-nicotianamine transporter YSL7 [Cucumis sativus] >KGN58517.1 hypothetical protein Csa_001368 [Cucumis sativus])

HSP 1 Score: 1337 bits (3461), Expect = 0.0
Identity = 684/684 (100.00%), Postives = 684/684 (100.00%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60
           MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF
Sbjct: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60

Query: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120
           IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS
Sbjct: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120

Query: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180
           GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK
Sbjct: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180

Query: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240
           MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD
Sbjct: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240

Query: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300
           GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR
Sbjct: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300

Query: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360
           KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS
Sbjct: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360

Query: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420
           SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA
Sbjct: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420

Query: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480
           PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD
Sbjct: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480

Query: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540
           LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM
Sbjct: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540

Query: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600
           YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA
Sbjct: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600

Query: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660
           IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG
Sbjct: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660

Query: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684
           VKAPLCMKFLSSSTNAKVDAFLEG
Sbjct: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684

BLAST of Cucsat.G12332 vs. NCBI nr
Match: XP_008461864.1 (PREDICTED: probable metal-nicotianamine transporter YSL7 [Cucumis melo] >KAA0052763.1 putative metal-nicotianamine transporter YSL7 [Cucumis melo var. makuwa] >TYK04193.1 putative metal-nicotianamine transporter YSL7 [Cucumis melo var. makuwa])

HSP 1 Score: 1301 bits (3368), Expect = 0.0
Identity = 660/684 (96.49%), Postives = 675/684 (98.68%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60
           MERNSSKK NEGESG+GTDGGG+RI+VEDAFKNLEVPSWRNQITFRALFTSF+LSIVFNF
Sbjct: 1   MERNSSKKANEGESGTGTDGGGDRIMVEDAFKNLEVPSWRNQITFRALFTSFVLSIVFNF 60

Query: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120
           IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSIL+QLGLMKQPFTRQENTVIQTCVVASS
Sbjct: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILEQLGLMKQPFTRQENTVIQTCVVASS 120

Query: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180
           GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK
Sbjct: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180

Query: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240
           MMI+KYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSF+FA+FQWFFAAAD
Sbjct: 181 MMILKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFLFAMFQWFFAAAD 240

Query: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300
           GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR
Sbjct: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300

Query: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360
           KGDWY+ASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFML QTFYSLSKQKSG GNADS
Sbjct: 301 KGDWYSASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLSQTFYSLSKQKSGCGNADS 360

Query: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420
             EI DYDARRR+EYFLKDQIPNWVALLGYVILAAISII VPLIFHQLKWYHVLVAY IA
Sbjct: 361 LSEINDYDARRRIEYFLKDQIPNWVALLGYVILAAISIITVPLIFHQLKWYHVLVAYAIA 420

Query: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480
           PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD
Sbjct: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480

Query: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540
           LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYN+GDPEGSYPAPYGLM
Sbjct: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNVGDPEGSYPAPYGLM 540

Query: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600
           YRGIALLGVEGVS+LPKNCLTLAICFFVGAIVINIIRD+LH+ ETK+RIYRFIPSPMCMA
Sbjct: 541 YRGIALLGVEGVSTLPKNCLTLAICFFVGAIVINIIRDILHRCETKYRIYRFIPSPMCMA 600

Query: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660
           IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG
Sbjct: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660

Query: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684
           VKAPLCMKFLSSSTNAKVDAFLEG
Sbjct: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684

BLAST of Cucsat.G12332 vs. NCBI nr
Match: XP_038877262.1 (probable metal-nicotianamine transporter YSL7 [Benincasa hispida])

HSP 1 Score: 1271 bits (3290), Expect = 0.0
Identity = 645/684 (94.30%), Postives = 667/684 (97.51%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60
           MERNSSKK NEGES +G DGG ERI+VE+AFKNLEVPSWRNQITFRAL TSFILSIVFNF
Sbjct: 1   MERNSSKKANEGESATGIDGG-ERIMVEEAFKNLEVPSWRNQITFRALLTSFILSIVFNF 60

Query: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120
           IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSIL Q GLMKQPFTRQENTVIQTCVVASS
Sbjct: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILAQFGLMKQPFTRQENTVIQTCVVASS 120

Query: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180
           GIAFSSGTASYLLGMSAK+AAQAEEGN+PINIKKLSVGWM+GFLFVVSFVGLFSIVPLRK
Sbjct: 121 GIAFSSGTASYLLGMSAKVAAQAEEGNIPINIKKLSVGWMMGFLFVVSFVGLFSIVPLRK 180

Query: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240
            MI+KYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFA+FQWFFAAAD
Sbjct: 181 TMILKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFAMFQWFFAAAD 240

Query: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300
           GCGFSSFPTFGL+AYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR
Sbjct: 241 GCGFSSFPTFGLEAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300

Query: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360
           KGDWY+ASLSASSLHGIQGYRVFIAI+MMLGDGLYHV FMLFQTFYSLSKQKSGS N DS
Sbjct: 301 KGDWYSASLSASSLHGIQGYRVFIAISMMLGDGLYHVFFMLFQTFYSLSKQKSGSENDDS 360

Query: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420
           SLE+TDYDA+RR EYFLKDQIPNWVA+LGYVILAAISII VP+IFHQLKWYH+LVAY IA
Sbjct: 361 SLEVTDYDAQRRTEYFLKDQIPNWVAMLGYVILAAISIITVPIIFHQLKWYHILVAYAIA 420

Query: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480
           PVLAFCNAYGCGLTDWSLASNYGKFAIIIFS+WVGLGNGGVIAGLASCGVMMSIVSTASD
Sbjct: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSAWVGLGNGGVIAGLASCGVMMSIVSTASD 480

Query: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540
           LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYN+GDPEGSYPAPYGLM
Sbjct: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNVGDPEGSYPAPYGLM 540

Query: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600
           YRGIALLGVEGVSSLPKNCLTLAICFF GAIVINI RDVLH+ ETK+R+YRFIPSPMCMA
Sbjct: 541 YRGIALLGVEGVSSLPKNCLTLAICFFFGAIVINITRDVLHRCETKYRVYRFIPSPMCMA 600

Query: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660
           IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAA+LALAG
Sbjct: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAVLALAG 660

Query: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684
           VKAPLCMKFLSSSTNAKVDAFLEG
Sbjct: 661 VKAPLCMKFLSSSTNAKVDAFLEG 683

BLAST of Cucsat.G12332 vs. NCBI nr
Match: XP_038905641.1 (probable metal-nicotianamine transporter YSL7 [Benincasa hispida])

HSP 1 Score: 1215 bits (3144), Expect = 0.0
Identity = 610/684 (89.18%), Postives = 653/684 (95.47%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60
           ME++ S   NEGES +      ER++VE+AF+NLEVPSWR+QITFRA+FTSFILSIVFNF
Sbjct: 1   MEKDPSNNGNEGESINT-----ERVMVEEAFRNLEVPSWRDQITFRAIFTSFILSIVFNF 60

Query: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120
           IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSIL+  GLMKQPFTRQENTVIQTCVVASS
Sbjct: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILNHFGLMKQPFTRQENTVIQTCVVASS 120

Query: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180
           GIAFSSGTASYLLGMSAKIAAQAEEG++PINIK+LSVGWM+GFLF+VSFVGLFSIVPLRK
Sbjct: 121 GIAFSSGTASYLLGMSAKIAAQAEEGSIPINIKRLSVGWMMGFLFIVSFVGLFSIVPLRK 180

Query: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240
           MMI+KYKL YPSGTATAYLINSFHTPKGAKLAKKQVAVLFK+FC SFVFALFQWFF AAD
Sbjct: 181 MMILKYKLPYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKTFCCSFVFALFQWFFTAAD 240

Query: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300
           GCGFSSFPTFGLQAYAKRFYFDFS+TYVGVGMICPFMVNLSLL GAIISWGIMWPLIEL+
Sbjct: 241 GCGFSSFPTFGLQAYAKRFYFDFSATYVGVGMICPFMVNLSLLFGAIISWGIMWPLIELK 300

Query: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360
           KGDWY+ASLSASSLHGIQGYRVFIAIAMMLGDGLYHV FMLFQTFYSL+K++S + N DS
Sbjct: 301 KGDWYSASLSASSLHGIQGYRVFIAIAMMLGDGLYHVFFMLFQTFYSLAKKRSSNENIDS 360

Query: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420
           S+E+TDYDA+RR EYFLKDQIPNWVA++GYV+LAAIS+I VPLIFHQLKWYH+LVAY IA
Sbjct: 361 SMEVTDYDAQRRSEYFLKDQIPNWVAMVGYVVLAAISVITVPLIFHQLKWYHILVAYAIA 420

Query: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480
           PVLAFCNAYGCGLTDWSLASNYGKFAIIIFS+WVGLGNGGVIAGLASCGVMMSIV+TASD
Sbjct: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSAWVGLGNGGVIAGLASCGVMMSIVATASD 480

Query: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540
           LMQDFKTGYLTL SPRSMF SQV GTA+GC+LSPLVFWFFFKAYN+GDP+GSYPAPYGLM
Sbjct: 481 LMQDFKTGYLTLTSPRSMFVSQVCGTAIGCILSPLVFWFFFKAYNVGDPDGSYPAPYGLM 540

Query: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600
           YRGIALLGVEGVSSLPKNCLTLAICFFVGA+VINIIRDVL K+ETK RIYRFIPSPMCMA
Sbjct: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAVVINIIRDVLQKYETKHRIYRFIPSPMCMA 600

Query: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660
           IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGE+APAVASGLICGESLW VPAAILALAG
Sbjct: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEYAPAVASGLICGESLWGVPAAILALAG 660

Query: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684
           VKAPLCMKFLSSS NA+VDAFL+G
Sbjct: 661 VKAPLCMKFLSSSANARVDAFLQG 679

BLAST of Cucsat.G12332 vs. NCBI nr
Match: XP_022153106.1 (probable metal-nicotianamine transporter YSL7 [Momordica charantia])

HSP 1 Score: 1199 bits (3102), Expect = 0.0
Identity = 598/662 (90.33%), Postives = 636/662 (96.07%), Query Frame = 0

Query: 23  ERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNFIVCKLNLTTGVIPSLNVAAGLL 82
           ERI+VE+AF+N EVP W+NQITFRA+FTSF+LSIVFNFIVCKLNLTTGVIPSLNVAAGLL
Sbjct: 11  ERIMVEEAFRNTEVPPWQNQITFRAIFTSFLLSIVFNFIVCKLNLTTGVIPSLNVAAGLL 70

Query: 83  GFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQ 142
           GFAILKGYTSIL   GLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQ
Sbjct: 71  GFAILKGYTSILKTFGLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQ 130

Query: 143 AEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRKMMIIKYKLTYPSGTATAYLINS 202
            EEGN PINIK+LSVGWM+GFLFVVSFVGLFSIVPLRKMMI+KYKLTYPSGTATAYLINS
Sbjct: 131 GEEGNTPINIKRLSVGWMMGFLFVVSFVGLFSIVPLRKMMILKYKLTYPSGTATAYLINS 190

Query: 203 FHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAADGCGFSSFPTFGLQAYAKRFYFD 262
           FHTPKGAKLAKKQVAVLFKSFCFSFVFA+FQWF+AAADGCGFSSFPTFGLQAYAKRFYFD
Sbjct: 191 FHTPKGAKLAKKQVAVLFKSFCFSFVFAMFQWFYAAADGCGFSSFPTFGLQAYAKRFYFD 250

Query: 263 FSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELRKGDWYNASLSASSLHGIQGYRV 322
           FSSTYVGVGMICPFMVN+SLLLGA+ISWGIMWPLIE RKG WY+ASLSASSLHGIQGYRV
Sbjct: 251 FSSTYVGVGMICPFMVNISLLLGAVISWGIMWPLIEQRKGIWYSASLSASSLHGIQGYRV 310

Query: 323 FIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADSSLEITDYDARRRMEYFLKDQIP 382
           FIAI+MMLGDGLYHV +MLFQTFYSL+KQKS S NA++S EITDYDA+RR EYFLKDQIP
Sbjct: 311 FIAISMMLGDGLYHVFYMLFQTFYSLAKQKSDSENANTSSEITDYDAQRRTEYFLKDQIP 370

Query: 383 NWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIAPVLAFCNAYGCGLTDWSLASNY 442
           NWVAL+GYV+LA IS+I VPLIFHQLKWYH+LVAY IAPVLAFCNAYGCGLTDWSLASNY
Sbjct: 371 NWVALIGYVVLAVISVITVPLIFHQLKWYHILVAYAIAPVLAFCNAYGCGLTDWSLASNY 430

Query: 443 GKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLTLASPRSMFFSQ 502
           GKFAIIIFS+WVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLTLASPRSMFFSQ
Sbjct: 431 GKFAIIIFSAWVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLTLASPRSMFFSQ 490

Query: 503 VAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLMYRGIALLGVEGVSSLPKNCLTL 562
           V GTAMGCVLSPLVFWFF+KAYN+GDPEGSYPAPYGLMYRGIALLGVEGVSSLPKNCLTL
Sbjct: 491 VCGTAMGCVLSPLVFWFFYKAYNVGDPEGSYPAPYGLMYRGIALLGVEGVSSLPKNCLTL 550

Query: 563 AICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMAIPFYLGAYFAIDMCVGSLILFI 622
           AICFFV +I +NIIRD+L K+E K+ IYRF+PSPMCMAIPFYLGAYFAIDMCVGSLILF+
Sbjct: 551 AICFFVASIAMNIIRDLLAKYERKYHIYRFVPSPMCMAIPFYLGAYFAIDMCVGSLILFL 610

Query: 623 WQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAGVKAPLCMKFLSSSTNAKVDAFL 682
           WQR++KV+A EFAPAVASGLICGESLWSVPAAILAL GVKAPLCMKFLSSSTN KVDAFL
Sbjct: 611 WQRRDKVRASEFAPAVASGLICGESLWSVPAAILALXGVKAPLCMKFLSSSTNDKVDAFL 670

Query: 683 EG 684
           +G
Sbjct: 671 QG 672

BLAST of Cucsat.G12332 vs. ExPASy TrEMBL
Match: A0A0A0LCX3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G654470 PE=4 SV=1)

HSP 1 Score: 1337 bits (3461), Expect = 0.0
Identity = 684/684 (100.00%), Postives = 684/684 (100.00%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60
           MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF
Sbjct: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60

Query: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120
           IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS
Sbjct: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120

Query: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180
           GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK
Sbjct: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180

Query: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240
           MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD
Sbjct: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240

Query: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300
           GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR
Sbjct: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300

Query: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360
           KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS
Sbjct: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360

Query: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420
           SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA
Sbjct: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420

Query: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480
           PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD
Sbjct: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480

Query: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540
           LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM
Sbjct: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540

Query: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600
           YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA
Sbjct: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600

Query: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660
           IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG
Sbjct: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660

Query: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684
           VKAPLCMKFLSSSTNAKVDAFLEG
Sbjct: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684

BLAST of Cucsat.G12332 vs. ExPASy TrEMBL
Match: A0A5D3BWT5 (Putative metal-nicotianamine transporter YSL7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1856G00190 PE=4 SV=1)

HSP 1 Score: 1301 bits (3368), Expect = 0.0
Identity = 660/684 (96.49%), Postives = 675/684 (98.68%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60
           MERNSSKK NEGESG+GTDGGG+RI+VEDAFKNLEVPSWRNQITFRALFTSF+LSIVFNF
Sbjct: 1   MERNSSKKANEGESGTGTDGGGDRIMVEDAFKNLEVPSWRNQITFRALFTSFVLSIVFNF 60

Query: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120
           IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSIL+QLGLMKQPFTRQENTVIQTCVVASS
Sbjct: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILEQLGLMKQPFTRQENTVIQTCVVASS 120

Query: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180
           GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK
Sbjct: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180

Query: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240
           MMI+KYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSF+FA+FQWFFAAAD
Sbjct: 181 MMILKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFLFAMFQWFFAAAD 240

Query: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300
           GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR
Sbjct: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300

Query: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360
           KGDWY+ASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFML QTFYSLSKQKSG GNADS
Sbjct: 301 KGDWYSASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLSQTFYSLSKQKSGCGNADS 360

Query: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420
             EI DYDARRR+EYFLKDQIPNWVALLGYVILAAISII VPLIFHQLKWYHVLVAY IA
Sbjct: 361 LSEINDYDARRRIEYFLKDQIPNWVALLGYVILAAISIITVPLIFHQLKWYHVLVAYAIA 420

Query: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480
           PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD
Sbjct: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480

Query: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540
           LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYN+GDPEGSYPAPYGLM
Sbjct: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNVGDPEGSYPAPYGLM 540

Query: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600
           YRGIALLGVEGVS+LPKNCLTLAICFFVGAIVINIIRD+LH+ ETK+RIYRFIPSPMCMA
Sbjct: 541 YRGIALLGVEGVSTLPKNCLTLAICFFVGAIVINIIRDILHRCETKYRIYRFIPSPMCMA 600

Query: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660
           IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG
Sbjct: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660

Query: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684
           VKAPLCMKFLSSSTNAKVDAFLEG
Sbjct: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684

BLAST of Cucsat.G12332 vs. ExPASy TrEMBL
Match: A0A1S3CG63 (probable metal-nicotianamine transporter YSL7 OS=Cucumis melo OX=3656 GN=LOC103500359 PE=4 SV=1)

HSP 1 Score: 1301 bits (3368), Expect = 0.0
Identity = 660/684 (96.49%), Postives = 675/684 (98.68%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTDGGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNF 60
           MERNSSKK NEGESG+GTDGGG+RI+VEDAFKNLEVPSWRNQITFRALFTSF+LSIVFNF
Sbjct: 1   MERNSSKKANEGESGTGTDGGGDRIMVEDAFKNLEVPSWRNQITFRALFTSFVLSIVFNF 60

Query: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASS 120
           IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSIL+QLGLMKQPFTRQENTVIQTCVVASS
Sbjct: 61  IVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILEQLGLMKQPFTRQENTVIQTCVVASS 120

Query: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180
           GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK
Sbjct: 121 GIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRK 180

Query: 181 MMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAAD 240
           MMI+KYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSF+FA+FQWFFAAAD
Sbjct: 181 MMILKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFLFAMFQWFFAAAD 240

Query: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300
           GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR
Sbjct: 241 GCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELR 300

Query: 301 KGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADS 360
           KGDWY+ASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFML QTFYSLSKQKSG GNADS
Sbjct: 301 KGDWYSASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLSQTFYSLSKQKSGCGNADS 360

Query: 361 SLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIA 420
             EI DYDARRR+EYFLKDQIPNWVALLGYVILAAISII VPLIFHQLKWYHVLVAY IA
Sbjct: 361 LSEINDYDARRRIEYFLKDQIPNWVALLGYVILAAISIITVPLIFHQLKWYHVLVAYAIA 420

Query: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480
           PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD
Sbjct: 421 PVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASD 480

Query: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLM 540
           LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYN+GDPEGSYPAPYGLM
Sbjct: 481 LMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNVGDPEGSYPAPYGLM 540

Query: 541 YRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMA 600
           YRGIALLGVEGVS+LPKNCLTLAICFFVGAIVINIIRD+LH+ ETK+RIYRFIPSPMCMA
Sbjct: 541 YRGIALLGVEGVSTLPKNCLTLAICFFVGAIVINIIRDILHRCETKYRIYRFIPSPMCMA 600

Query: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660
           IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG
Sbjct: 601 IPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAG 660

Query: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684
           VKAPLCMKFLSSSTNAKVDAFLEG
Sbjct: 661 VKAPLCMKFLSSSTNAKVDAFLEG 684

BLAST of Cucsat.G12332 vs. ExPASy TrEMBL
Match: A0A6J1DJP2 (probable metal-nicotianamine transporter YSL7 OS=Momordica charantia OX=3673 GN=LOC111020688 PE=4 SV=1)

HSP 1 Score: 1199 bits (3102), Expect = 0.0
Identity = 598/662 (90.33%), Postives = 636/662 (96.07%), Query Frame = 0

Query: 23  ERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFNFIVCKLNLTTGVIPSLNVAAGLL 82
           ERI+VE+AF+N EVP W+NQITFRA+FTSF+LSIVFNFIVCKLNLTTGVIPSLNVAAGLL
Sbjct: 11  ERIMVEEAFRNTEVPPWQNQITFRAIFTSFLLSIVFNFIVCKLNLTTGVIPSLNVAAGLL 70

Query: 83  GFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQ 142
           GFAILKGYTSIL   GLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQ
Sbjct: 71  GFAILKGYTSILKTFGLMKQPFTRQENTVIQTCVVASSGIAFSSGTASYLLGMSAKIAAQ 130

Query: 143 AEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLRKMMIIKYKLTYPSGTATAYLINS 202
            EEGN PINIK+LSVGWM+GFLFVVSFVGLFSIVPLRKMMI+KYKLTYPSGTATAYLINS
Sbjct: 131 GEEGNTPINIKRLSVGWMMGFLFVVSFVGLFSIVPLRKMMILKYKLTYPSGTATAYLINS 190

Query: 203 FHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAADGCGFSSFPTFGLQAYAKRFYFD 262
           FHTPKGAKLAKKQVAVLFKSFCFSFVFA+FQWF+AAADGCGFSSFPTFGLQAYAKRFYFD
Sbjct: 191 FHTPKGAKLAKKQVAVLFKSFCFSFVFAMFQWFYAAADGCGFSSFPTFGLQAYAKRFYFD 250

Query: 263 FSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIELRKGDWYNASLSASSLHGIQGYRV 322
           FSSTYVGVGMICPFMVN+SLLLGA+ISWGIMWPLIE RKG WY+ASLSASSLHGIQGYRV
Sbjct: 251 FSSTYVGVGMICPFMVNISLLLGAVISWGIMWPLIEQRKGIWYSASLSASSLHGIQGYRV 310

Query: 323 FIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNADSSLEITDYDARRRMEYFLKDQIP 382
           FIAI+MMLGDGLYHV +MLFQTFYSL+KQKS S NA++S EITDYDA+RR EYFLKDQIP
Sbjct: 311 FIAISMMLGDGLYHVFYMLFQTFYSLAKQKSDSENANTSSEITDYDAQRRTEYFLKDQIP 370

Query: 383 NWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVIAPVLAFCNAYGCGLTDWSLASNY 442
           NWVAL+GYV+LA IS+I VPLIFHQLKWYH+LVAY IAPVLAFCNAYGCGLTDWSLASNY
Sbjct: 371 NWVALIGYVVLAVISVITVPLIFHQLKWYHILVAYAIAPVLAFCNAYGCGLTDWSLASNY 430

Query: 443 GKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLTLASPRSMFFSQ 502
           GKFAIIIFS+WVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLTLASPRSMFFSQ
Sbjct: 431 GKFAIIIFSAWVGLGNGGVIAGLASCGVMMSIVSTASDLMQDFKTGYLTLASPRSMFFSQ 490

Query: 503 VAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGLMYRGIALLGVEGVSSLPKNCLTL 562
           V GTAMGCVLSPLVFWFF+KAYN+GDPEGSYPAPYGLMYRGIALLGVEGVSSLPKNCLTL
Sbjct: 491 VCGTAMGCVLSPLVFWFFYKAYNVGDPEGSYPAPYGLMYRGIALLGVEGVSSLPKNCLTL 550

Query: 563 AICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCMAIPFYLGAYFAIDMCVGSLILFI 622
           AICFFV +I +NIIRD+L K+E K+ IYRF+PSPMCMAIPFYLGAYFAIDMCVGSLILF+
Sbjct: 551 AICFFVASIAMNIIRDLLAKYERKYHIYRFVPSPMCMAIPFYLGAYFAIDMCVGSLILFL 610

Query: 623 WQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALAGVKAPLCMKFLSSSTNAKVDAFL 682
           WQR++KV+A EFAPAVASGLICGESLWSVPAAILAL GVKAPLCMKFLSSSTN KVDAFL
Sbjct: 611 WQRRDKVRASEFAPAVASGLICGESLWSVPAAILALXGVKAPLCMKFLSSSTNDKVDAFL 670

Query: 683 EG 684
           +G
Sbjct: 671 QG 672

BLAST of Cucsat.G12332 vs. ExPASy TrEMBL
Match: A0A6J1KGN4 (probable metal-nicotianamine transporter YSL7 OS=Cucurbita maxima OX=3661 GN=LOC111493049 PE=4 SV=1)

HSP 1 Score: 1191 bits (3081), Expect = 0.0
Identity = 605/683 (88.58%), Postives = 641/683 (93.85%), Query Frame = 0

Query: 1   MERNSSKKENEGESGSGTD-GGGERIVVEDAFKNLEVPSWRNQITFRALFTSFILSIVFN 60
           MERN SK+    ES   ++  G ER++VEDAF+N EVPSW+NQIT RA+ TSFILSIVFN
Sbjct: 1   MERNPSKQMAGNESDYTSEIEGRERVMVEDAFRNTEVPSWKNQITVRAIVTSFILSIVFN 60

Query: 61  FIVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILDQLGLMKQPFTRQENTVIQTCVVAS 120
           FIVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSIL Q GLMK PFTRQENTVIQTCVVAS
Sbjct: 61  FIVCKLNLTTGVIPSLNVAAGLLGFAILKGYTSILSQFGLMKLPFTRQENTVIQTCVVAS 120

Query: 121 SGIAFSSGTASYLLGMSAKIAAQAEEGNMPINIKKLSVGWMIGFLFVVSFVGLFSIVPLR 180
           SGIAFSSGTASYLLGMS KIAAQAEEGN+PINIKKLSVGWM+GFLFVVSFVGLFSIVPLR
Sbjct: 121 SGIAFSSGTASYLLGMSGKIAAQAEEGNIPINIKKLSVGWMMGFLFVVSFVGLFSIVPLR 180

Query: 181 KMMIIKYKLTYPSGTATAYLINSFHTPKGAKLAKKQVAVLFKSFCFSFVFALFQWFFAAA 240
           KMMI+KYKLTYPSGTATAYLINSFHTPKGAKLAK+QVAVLFKSFCFSF FALFQWFFAAA
Sbjct: 181 KMMILKYKLTYPSGTATAYLINSFHTPKGAKLAKRQVAVLFKSFCFSFSFALFQWFFAAA 240

Query: 241 DGCGFSSFPTFGLQAYAKRFYFDFSSTYVGVGMICPFMVNLSLLLGAIISWGIMWPLIEL 300
           DGCGF+SFPTFGLQAY KRFYFDFSSTYVGVGMICPFMVN+SLLLGAIISWG+MWPLIE 
Sbjct: 241 DGCGFASFPTFGLQAYEKRFYFDFSSTYVGVGMICPFMVNISLLLGAIISWGVMWPLIEA 300

Query: 301 RKGDWYNASLSASSLHGIQGYRVFIAIAMMLGDGLYHVCFMLFQTFYSLSKQKSGSGNAD 360
           RKGDWY+ASLSA+SLHGIQGYRVF AIAMMLGDGLYHV FMLFQTFYSL++QK    + D
Sbjct: 301 RKGDWYSASLSATSLHGIQGYRVFTAIAMMLGDGLYHVFFMLFQTFYSLAQQKLSHDSGD 360

Query: 361 SSLEITDYDARRRMEYFLKDQIPNWVALLGYVILAAISIIAVPLIFHQLKWYHVLVAYVI 420
           SSL+ TDYDA+RR+E+F KDQIPNWVA+LGY ILA ISIIAVPLIFHQLKWYHVLVAY I
Sbjct: 361 SSLDATDYDAKRRIEHFTKDQIPNWVAMLGYGILAVISIIAVPLIFHQLKWYHVLVAYAI 420

Query: 421 APVLAFCNAYGCGLTDWSLASNYGKFAIIIFSSWVGLGNGGVIAGLASCGVMMSIVSTAS 480
           APVLAFCNAYGCGLTDWSLASNYGKF+IIIFSSWVGL NGGVIAGLASCGVMMSIVSTAS
Sbjct: 421 APVLAFCNAYGCGLTDWSLASNYGKFSIIIFSSWVGLDNGGVIAGLASCGVMMSIVSTAS 480

Query: 481 DLMQDFKTGYLTLASPRSMFFSQVAGTAMGCVLSPLVFWFFFKAYNIGDPEGSYPAPYGL 540
           DLMQDFKTGYLTLASPRSMFFSQV GTAMGC LSPLVFWFFFKAYN+GDPEGSYPAPYGL
Sbjct: 481 DLMQDFKTGYLTLASPRSMFFSQVCGTAMGCFLSPLVFWFFFKAYNVGDPEGSYPAPYGL 540

Query: 541 MYRGIALLGVEGVSSLPKNCLTLAICFFVGAIVINIIRDVLHKFETKFRIYRFIPSPMCM 600
           MYRGIALLGVEGVSSLP+NCLTLAICFFV AIVINIIR+ L K +T++R YRFIPSPMCM
Sbjct: 541 MYRGIALLGVEGVSSLPRNCLTLAICFFVAAIVINIIREFLQKVDTRYRTYRFIPSPMCM 600

Query: 601 AIPFYLGAYFAIDMCVGSLILFIWQRKNKVKAGEFAPAVASGLICGESLWSVPAAILALA 660
           AIPFYLGAYFAIDMCVGSLILF+WQR+NK+KA EFAPAVASGLICGESLWSVPAAILALA
Sbjct: 601 AIPFYLGAYFAIDMCVGSLILFLWQRRNKIKASEFAPAVASGLICGESLWSVPAAILALA 660

Query: 661 GVKAPLCMKFLSSSTNAKVDAFL 682
           GVKAPLCMKFLSSSTN+KVDAFL
Sbjct: 661 GVKAPLCMKFLSSSTNSKVDAFL 683