Cucsat.G12324 (gene) Cucumber (B10) v3

BLAST of Cucsat.G12324 vs. ExPASy Swiss-Prot
Match: A0A1D6F9Y9 (Trimethyltridecatetraene synthase OS=Zea mays OX=4577 GN=CYP92C6 PE=1 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 2.0e-137
Identity = 254/506 (50.20%), Postives = 344/506 (67.98%), Query Frame = 0

Query: 44  RRRKLNLPPGPKPWPIIGNLD-LIGSL-PHQSIHQLSKKYGPIMHLRFGSFPVVVGSSVE 103
           R + LNLPPGP+ WP+IG+L  L G+L PH+++  L+ ++GP+MHLR GS+  VV SS +
Sbjct: 26  RSKPLNLPPGPRGWPVIGSLGALAGALPPHRALAALAARHGPLMHLRLGSYHAVVASSAD 85

Query: 104 MAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNITWSQYGPYWRQLRKMCLMELFSARRLDS 163
            A++VLK  DL F  RP+TAAG+  +Y Y  I  + YG YWR  RK+C  ELFSARR+DS
Sbjct: 86  TARLVLKVHDLAFADRPRTAAGEVASYGYLGIVHTPYGAYWRMARKLCATELFSARRVDS 145

Query: 164 YEYIRKEEMNGLIREIYKSCGE-VIKVKDYLFALSLNVISRMVLGKRYTDESSESGIVSP 223
           +E +R +EM  L R +++  G   + V++++   +L  I RM +G+++   S   G    
Sbjct: 146 FERVRAQEMRALARGLFECAGRGAVAVREHVAGATLRNILRMAVGEKW---SGCYGSPEG 205

Query: 224 DEFRKMMDELFLLNG-VLNIGDSIPWMDFLDLQGYVKRMKGLSKKLDRFLEHVLDEH--- 283
           + FR+ +DE F + G V N+G+ +PW+ +LDLQG V+RMK L  + DRF E +LD+H   
Sbjct: 206 EAFRRTLDEAFAVTGAVSNVGEWVPWLGWLDLQGCVRRMKRLRAQYDRFFEQILDDHDHK 265

Query: 284 ---KER-RKGVENYVA-KDMVDVLLQLA----DHPDL--EVKLERHGVKAFTQDLLGGGT 343
              +ER R G  +  A  D+VDVLL+L     D P+     +L R GVKAF QD++ GGT
Sbjct: 266 KTRRERGRPGAGDSAADDDLVDVLLRLVEEDEDRPETTEASRLTRDGVKAFVQDIVAGGT 325

Query: 344 ETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPYINAIAKETMRLH 403
           E+S +TIEWAMSELL+ P     AT EL++VIG  RWV E+D+ +LPYI+A+ KETMRLH
Sbjct: 326 ESSAVTIEWAMSELLRRPDALRAATAELDRVIGHGRWVTERDLPDLPYIDAVVKETMRLH 385

Query: 404 PVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKN-PHAFDPDRFIENS-- 463
           PV P+LVP  A E   +AGYD+  G RVLVNVW I RD   W + P AF P+RF+  S  
Sbjct: 386 PVGPLLVPHHAREHTVVAGYDVPAGARVLVNVWAIARDPASWPDAPDAFRPERFLNGSSG 445

Query: 464 -RVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDMEKEDLNMEE 523
             VDV+G  FELLPFG+GRRMCP + L +K++ + +ANL+HGF W+LP  M  ED++MEE
Sbjct: 446 ASVDVRGAHFELLPFGAGRRMCPAHGLAMKLVTAGVANLVHGFAWRLPDGMAPEDVSMEE 505

Query: 524 IFGLSTPKKYPLDAVAEPRLPPHLYS 528
           +FGLST +K PL AVAEPRLP HLY+
Sbjct: 506 LFGLSTRRKVPLVAVAEPRLPAHLYT 528

BLAST of Cucsat.G12324 vs. ExPASy Swiss-Prot
Match: A0A1D6HSP4 (Dimethylnonatriene synthase OS=Zea mays OX=4577 GN=CYP92C5 PE=1 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.6e-129
Identity = 242/531 (45.57%), Postives = 347/531 (65.35%), Query Frame = 0

Query: 14  MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRK-LNLPPGPKPWPIIGNLD-LIGSL-P 73
           ME+    S    +  +I   L  ++ S   RR K L LPPGP+ WP++G+L  L G+L P
Sbjct: 1   MELASTMSVAMALAAAIFVVLCSVVASARGRREKALKLPPGPRGWPVLGSLGALAGALPP 60

Query: 74  HQSIHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNY 133
           H+++  L+ ++GP+MHLR GS+  VV SS + A++VL+T D     RP TAAG+ T+Y Y
Sbjct: 61  HRALAALAARHGPLMHLRLGSYHTVVASSADAARLVLRTHDSALADRPDTAAGEITSYGY 120

Query: 134 SNITWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCG--EVIKVK 193
             I  +  G YWR  R++C  ELFSARR++S++ +R +EM  L R ++        + V+
Sbjct: 121 LGIVHTPRGAYWRMARRLCATELFSARRVESFQDVRAQEMRALARGLFGCAAGRRAVAVR 180

Query: 194 DYLFALSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNG-VLNIGDSIPWMD 253
           +++   ++  I RM +G+++   S   G    + FR+ +DE F   G V N+G+ +PW+ 
Sbjct: 181 EHVAGATMRNILRMAVGEKW---SGCYGSPEGEAFRRSLDEAFAATGAVSNVGEWVPWLG 240

Query: 254 FLDLQGYVKRMKGLSKKLDRFLEHVLDEHKERRKGVE----NYVAKDMVDVLLQLAD--- 313
           +LD+QG+ ++MK L    D F E +L +H+ERR+  +     +VA D+VDVLLQL++   
Sbjct: 241 WLDVQGFKRKMKRLHDLHDHFYEKILVDHEERRRLAQASGGEFVATDLVDVLLQLSEEST 300

Query: 314 --HPDLEVKLERHGVKAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIG 373
               + E +L R GVKA  QD++ GGTE+S +TIEWAM+ELL++P+   KAT EL++V+G
Sbjct: 301 KLESESEARLPRDGVKALIQDIIAGGTESSAVTIEWAMAELLRHPEAMAKATDELDRVVG 360

Query: 374 KERWVEEKDMINLPYINAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVW 433
             RWV E+D+  L YI+A+ KET+RLHPV P+LVP  A E   +AGYD+  G RVLVN W
Sbjct: 361 SGRWVAERDLPELHYIDAVVKETLRLHPVGPLLVPHYARERTVVAGYDVPAGARVLVNAW 420

Query: 434 TIGRDQTVWKN-PHAFDPDRFI-ENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILST 493
            I RD   W + P AF P+RF+   + VDV+G  FELLPFGSGRR+CP Y L +K++ + 
Sbjct: 421 AIARDPASWPDAPDAFQPERFLGAAAAVDVRGAHFELLPFGSGRRICPAYDLAMKLVAAG 480

Query: 494 LANLLHGFNWKLPGDMEKEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYS 528
           +ANL+HGF W+LP  +  ED++MEE  GLST +K PL AVAEPRLP HLYS
Sbjct: 481 VANLVHGFAWRLPDGVAAEDVSMEEHVGLSTRRKVPLFAVAEPRLPVHLYS 528

BLAST of Cucsat.G12324 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 426.4 bits (1095), Expect = 4.7e-118
Identity = 220/504 (43.65%), Postives = 317/504 (62.90%), Query Frame = 0

Query: 23  LSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQSIHQLSKKYG 82
           +S +  +I  T  LL L+   R +K NLPP P   PIIGNL  +G+LPH+S+  L+ + G
Sbjct: 5   VSLLFLAIALTFFLLKLNE-KREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELG 64

Query: 83  PIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNITWSQYGPYW 142
           P++ L  G  P ++ S+ E+A+ +LKT DL F  RP T A +   Y+ +++ +S YG YW
Sbjct: 65  PLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYW 124

Query: 143 RQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSC--GEVIKVKDYLFALSLNVIS 202
           RQ+RK+C++EL S +R++SY  IR+EE+  ++  I +SC  GE + + + L  LS   I+
Sbjct: 125 RQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTIT 184

Query: 203 RMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLD-LQGYVKRMK 262
           R+  GK+Y  E         ++F  +  EL  L G   +GD  P   ++D L G   R+K
Sbjct: 185 RVAFGKKYEGEEERK-----NKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLK 244

Query: 263 GLSKKLDRFLEHVLDEHKERRK--GVENYVAKDMVDVLLQLADHPDLEVKLERHGVKAFT 322
               +LD F++HV+D+H   RK  G +    KD+VDVLL L     L V L R+ +KA  
Sbjct: 245 RNHGELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVI 304

Query: 323 QDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPYINAI 382
            D+  GGT+T+ +T+EWAM+EL+K+P +  KA  E+ +V+GK+  VEE+D+  L Y+  I
Sbjct: 305 LDMFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLI 364

Query: 383 AKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAFDPDR 442
            KET+RLHPV P+LVPR +  D  I GY I   TRV +N W IGRD   W+N   F P+R
Sbjct: 365 IKETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPER 424

Query: 443 FIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDMEKEDL 502
           F+ NS VD KGQDF+L+PFG+GRR CPG + G+  +  +LANLL+ FNW+LPGD+ KEDL
Sbjct: 425 FVNNS-VDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDL 484

Query: 503 NMEEIFGLSTPKKYPLDAVAEPRL 522
           +M E  G++   K+PL  VA+  L
Sbjct: 485 DMSEAVGITVHMKFPLQLVAKRHL 501

BLAST of Cucsat.G12324 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 4.9e-115
Identity = 209/509 (41.06%), Postives = 319/509 (62.67%), Query Frame = 0

Query: 25  YVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQSIHQLSKKYGPI 84
           +++T ++++  + LL R    +   LPPGP  WPI+GNL  +G+ PHQ++  LSK+YGP+
Sbjct: 8   WISTLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQYGPL 67

Query: 85  MHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNITWSQYGPYWRQ 144
             LR GS  VVV SS  +A   L+T D+NF  RP  +  ++  YNY ++ ++ YGP WR 
Sbjct: 68  FRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRM 127

Query: 145 LRKMCLMELFSARRLDSYEYIRKEEMNGLIREI--YKSCGEVIKVKDYLFALSLNVISRM 204
           LRK+C + LFS + LD    +R+ E+  L+R +  +   G ++ +   L   + N ++R 
Sbjct: 128 LRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALARA 187

Query: 205 VLGKR-YTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQGYVKRMKGL 264
           +LG+R + DE ++  +   DEF++M+ EL  L GV N+GD +P + +LDLQG V +MK L
Sbjct: 188 MLGRRVFADEDAQ--LAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRL 247

Query: 265 SKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLAD-HPDLEVKLERHGVKAFTQDL 324
            ++ D FL+ V++E++   K      + D++ VL++L +   + E+KL    +KA   +L
Sbjct: 248 HRRYDAFLDRVIEENQANAK------SGDLLSVLIRLKEADAEGEIKLNNTDIKALLLNL 307

Query: 325 LGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPYINAIAKE 384
              GT+TS+ T+EW ++EL+++P I  K   EL+ VIG++R V E D+ NLPY+ A+ KE
Sbjct: 308 FTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVKE 367

Query: 385 TMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAFDPDRFI- 444
           T RLHP  P+ +PRMA E+C + GY I K   +LVNVW+IGRD  VW +P  F P RF+ 
Sbjct: 368 TFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLP 427

Query: 445 --ENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDMEKEDL 504
             E   VDVKG DFE++PFG+GRR+C G SLGL+++    A ++H ++W LP   E + L
Sbjct: 428 GGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQKL 487

Query: 505 NMEEIFGLSTPKKYPLDAVAEPRLPPHLY 527
           +MEE +GL+  +  PL     PRL    Y
Sbjct: 488 DMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of Cucsat.G12324 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 3.2e-114
Identity = 213/513 (41.52%), Postives = 317/513 (61.79%), Query Frame = 0

Query: 23  LSYVTTSIIATLALLLLSRCLRRRK--LNLPPGPKPWPIIGNLDLIGSLPHQSIHQLSKK 82
           LS +  ++I +  L  + R   R++  L LPPGPKPWPIIGNL  +G  PHQS   +++ 
Sbjct: 4   LSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMAQT 63

Query: 83  YGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNITWSQYGP 142
           YGP+M+L+ G   VVV +S  +A   LKT D NF  RP  +  ++  YNY ++ ++ YGP
Sbjct: 64  YGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGP 123

Query: 143 YWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFALSLNVIS 202
            WR LRK+C + LFS + LD + ++R++E+  L R +  +  + +K+   L   + N ++
Sbjct: 124 RWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALA 183

Query: 203 RMVLGKRYTDESSESGIVSPD--EFRKMMDELFLLNGVLNIGDSIPWMDFLDLQGYVKRM 262
           R++LGKR   +   SG V P   EF+ M+ E+ ++ GV NIGD IP +++LD+QG   +M
Sbjct: 184 RVMLGKRVFADG--SGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKM 243

Query: 263 KGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLA--DHPDLEVKLERHGVKAF 322
           K L  + D FL  +L+EHK +  G      KD++  L+ L   D  +   KL    +KA 
Sbjct: 244 KKLHARFDAFLTDILEEHKGKIFG----EMKDLLSTLISLKNDDADNDGGKLTDTEIKAL 303

Query: 323 TQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPYINA 382
             +L   GT+TS+ T+EWA++EL++NPKI  +A  E++KV+G++R V E D+  L Y+ A
Sbjct: 304 LLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEA 363

Query: 383 IAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAFDPD 442
           I KET RLHP  P+ +PR+A E C+I GY I KG+ +L+NVW I RD   W +P  F P+
Sbjct: 364 IVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPE 423

Query: 443 RFI---ENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 502
           RF+   E  +VDV+G DFE++PFG+GRR+C G +LG++++   +A L+H FNW L     
Sbjct: 424 RFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQL 483

Query: 503 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLY 527
            E LNMEE +GL+  +  PL     PRL    Y
Sbjct: 484 PEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510

BLAST of Cucsat.G12324 vs. NCBI nr
Match: XP_004148586.3 (trimethyltridecatetraene synthase [Cucumis sativus] >XP_031745350.1 trimethyltridecatetraene synthase-like [Cucumis sativus] >KAE8637308.1 hypothetical protein CSA_004523 [Cucumis sativus] >KAE8650901.1 hypothetical protein Csa_001942 [Cucumis sativus])

HSP 1 Score: 1050 bits (2715), Expect = 0.0
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 14  MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS 73
           MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS
Sbjct: 1   MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS 60

Query: 74  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI 133
           IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI
Sbjct: 61  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI 120

Query: 134 TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 193
           TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA
Sbjct: 121 TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 180

Query: 194 LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 253
           LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG
Sbjct: 181 LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 240

Query: 254 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV 313
           YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV
Sbjct: 241 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV 300

Query: 314 KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY 373
           KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY
Sbjct: 301 KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY 360

Query: 374 INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 433
           INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF
Sbjct: 361 INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 420

Query: 434 DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 493
           DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME
Sbjct: 421 DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 480

Query: 494 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 529
           KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL
Sbjct: 481 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 516

BLAST of Cucsat.G12324 vs. NCBI nr
Match: XP_004148565.3 (LOW QUALITY PROTEIN: trimethyltridecatetraene synthase [Cucumis sativus])

HSP 1 Score: 977 bits (2525), Expect = 0.0
Identity = 477/516 (92.44%), Postives = 495/516 (95.93%), Query Frame = 0

Query: 14  MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS 73
           MEMQP PSCLSY+T  I ATLA+LLLSR LRRRKLNLPPGPKPWP+IGNLDLIGSLPHQS
Sbjct: 1   MEMQPTPSCLSYITPWI-ATLAILLLSRRLRRRKLNLPPGPKPWPVIGNLDLIGSLPHQS 60

Query: 74  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI 133
           IHQLSKKYGPIMHLRFGSFP+VVGSSVEMAK+ LKTQDLNF  RPKT AGKYTTYN+SNI
Sbjct: 61  IHQLSKKYGPIMHLRFGSFPIVVGSSVEMAKLFLKTQDLNFASRPKTTAGKYTTYNHSNI 120

Query: 134 TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 193
           TWSQYGPYW QLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA
Sbjct: 121 TWSQYGPYWXQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 180

Query: 194 LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 253
           +SLNVISRMVLGK+YTDE SESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG
Sbjct: 181 VSLNVISRMVLGKKYTDEPSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 240

Query: 254 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV 313
           YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLAD PDLEVKLERHGV
Sbjct: 241 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADDPDLEVKLERHGV 300

Query: 314 KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY 373
           KAFTQDLLGGG E+ST+T+EW +SELLK P+I NKAT ELNKVIGKERWVEEKDMINLPY
Sbjct: 301 KAFTQDLLGGGIESSTVTVEWTISELLKTPEILNKATEELNKVIGKERWVEEKDMINLPY 360

Query: 374 INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 433
           INAIAKETMRLHPV PMLVPRMA EDCQ+AGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF
Sbjct: 361 INAIAKETMRLHPVAPMLVPRMAREDCQVAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 420

Query: 434 DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 493
           DPDRF+EN+ +DVKG+DFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME
Sbjct: 421 DPDRFMENNCIDVKGEDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 480

Query: 494 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 529
           KEDLNMEE FGLSTPKKYPLDAVAEPRLPPHLYSLL
Sbjct: 481 KEDLNMEESFGLSTPKKYPLDAVAEPRLPPHLYSLL 515

BLAST of Cucsat.G12324 vs. NCBI nr
Match: XP_008463991.1 (PREDICTED: cytochrome P450 71A1-like [Cucumis melo] >KAA0065634.1 cytochrome P450 71A1-like [Cucumis melo var. makuwa] >TYK07203.1 cytochrome P450 71A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 942 bits (2435), Expect = 0.0
Identity = 462/516 (89.53%), Postives = 489/516 (94.77%), Query Frame = 0

Query: 14  MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS 73
           MEM  IPSCLSY+T  I A LALLLLS  LRRRKLNLPPGPKPWP+IGNL LIGSLPHQS
Sbjct: 1   MEMPLIPSCLSYITAWI-AALALLLLSYRLRRRKLNLPPGPKPWPVIGNLHLIGSLPHQS 60

Query: 74  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI 133
           IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKI LKTQDLNFV RPKTAAGKYTTYNYS+I
Sbjct: 61  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDI 120

Query: 134 TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 193
           TWSQYGPYWRQ RKMCLMELFSARRLDSYEYIRKEEMNGL+REIYKSCGEVIKVKDYL  
Sbjct: 121 TWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNGLVREIYKSCGEVIKVKDYLST 180

Query: 194 LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 253
           +SLNVISRMVLGK+YTDESSE+GIVSPDEF+KM+DELFLL+GVLNIGDSIPW+DFLDLQG
Sbjct: 181 VSLNVISRMVLGKKYTDESSENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQG 240

Query: 254 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV 313
           YVKRMK LSKK DRFLEHVLDEH ERRKGV+NYVAKDMVDVLLQLAD P+LEVKLERHGV
Sbjct: 241 YVKRMKALSKKFDRFLEHVLDEHNERRKGVQNYVAKDMVDVLLQLADDPNLEVKLERHGV 300

Query: 314 KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY 373
           KAFTQDL+ GGTE+S +T+EWA+SELLKNP+IFNKA  ELN+VIGKERWVEEKD++NLPY
Sbjct: 301 KAFTQDLIAGGTESSAVTVEWAISELLKNPEIFNKAIEELNEVIGKERWVEEKDIVNLPY 360

Query: 374 INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 433
           INAIAKETMRLHPV PMLVPRMA EDCQIAGYDIAKGTRVLVNVWTIGRD TVW+NPHAF
Sbjct: 361 INAIAKETMRLHPVAPMLVPRMAREDCQIAGYDIAKGTRVLVNVWTIGRDPTVWRNPHAF 420

Query: 434 DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 493
           DPDRF+E+S +DVKGQDFELLPFGSGRRMCPGYSLGLKVI S+LANLLHGFNWKLPGDME
Sbjct: 421 DPDRFMESS-IDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFNWKLPGDME 480

Query: 494 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 529
           KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL
Sbjct: 481 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 514

BLAST of Cucsat.G12324 vs. NCBI nr
Match: XP_038877760.1 (trimethyltridecatetraene synthase-like [Benincasa hispida])

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 440/515 (85.44%), Postives = 481/515 (93.40%), Query Frame = 0

Query: 14  MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS 73
           MEMQ +PS +SY    + ATLALLLLSR L RRKLNLPPGP+PWP+IGNL+LIGSLPHQS
Sbjct: 1   MEMQ-LPSWVSYAAAWM-ATLALLLLSRRLCRRKLNLPPGPRPWPLIGNLNLIGSLPHQS 60

Query: 74  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI 133
           IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKI LKT DLNFV RPKTAAGKYTTYNYS+I
Sbjct: 61  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDI 120

Query: 134 TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 193
           TWSQYGPYWRQ RKMCLMELFSARRLDSYEYIRKEEMN L+++IYKS G+VIK+KDYL  
Sbjct: 121 TWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLKDIYKSRGQVIKLKDYLST 180

Query: 194 LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 253
           +SLNVISRMVLG++YTDES E+ IVSPDEF+KM+DELFLL+GVLNIGDSIPW+DFLDLQG
Sbjct: 181 VSLNVISRMVLGRKYTDES-ENAIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQG 240

Query: 254 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV 313
           YVKRMK LSKK DRFLEHVLDEH ERRKGVENYVAKDMVDVLLQ AD P+LEVKLERHGV
Sbjct: 241 YVKRMKALSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQQADDPNLEVKLERHGV 300

Query: 314 KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY 373
           KAFTQDL+ GGTE+S +TIEWA+SELLK P+IF+KAT EL++VIG+ERWVEEKD++NLPY
Sbjct: 301 KAFTQDLIAGGTESSAVTIEWAISELLKQPEIFDKATEELDRVIGRERWVEEKDIVNLPY 360

Query: 374 INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 433
           INAIAKETMRLHPV PMLVPRM  EDCQIAGYDIAKGTRVLVNVWTIGRD TVWKNP+AF
Sbjct: 361 INAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGRDPTVWKNPYAF 420

Query: 434 DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 493
           DP+RF+ENS +DVKGQDFELLPFGSGRRMCPGYSLGLKVI S+LANLLHGF+WKLPGDME
Sbjct: 421 DPERFMENS-IDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFSWKLPGDME 480

Query: 494 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSL 528
           KEDLN+EEIFGLSTPKK+PLDAVA+PRLPPHLYSL
Sbjct: 481 KEDLNLEEIFGLSTPKKFPLDAVADPRLPPHLYSL 511

BLAST of Cucsat.G12324 vs. NCBI nr
Match: XP_008460308.1 (PREDICTED: cytochrome P450 71A1-like [Cucumis melo])

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 428/504 (84.92%), Postives = 462/504 (91.67%), Query Frame = 0

Query: 25  YVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQSIHQLSKKYGPI 84
           YV  + IATLA+LLLSR +RRRKLNLPPGPKPWP+IGNLDLIGSLPHQSIHQLSKKYGPI
Sbjct: 7   YVVVAWIATLAILLLSRRIRRRKLNLPPGPKPWPLIGNLDLIGSLPHQSIHQLSKKYGPI 66

Query: 85  MHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNITWSQYGPYWRQ 144
           MHLRFGSFPVVVGSSVEMAKI LKTQDLNFV RPKTAAGKYTTYNYS+ITWSQYGPYWRQ
Sbjct: 67  MHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYWRQ 126

Query: 145 LRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFALSLNVISRMVL 204
            RKMCLMELFSARRLDSYEYIRKEEMN L+REIYKSCGEVIK+KDYL  +SLNVISRMVL
Sbjct: 127 ARKMCLMELFSARRLDSYEYIRKEEMNALLREIYKSCGEVIKIKDYLSTVSLNVISRMVL 186

Query: 205 GKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQGYVKRMKGLSKK 264
           GK+YTDES E+GIVSPDEF+KM+DELFLL+GVLNIGDSIPW+DFLDLQGYVKRMK LSKK
Sbjct: 187 GKKYTDES-ENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKALSKK 246

Query: 265 LDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGVKAFTQDLLGGG 324
            DRFLEHVLDEH ERRKGVE+YVAKDMVDVLLQLAD PDLEVKLERHGVKAFTQDL+ GG
Sbjct: 247 FDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQDLIAGG 306

Query: 325 TETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPYINAIAKETMRL 384
           TE+S +T+EWAMSELLK P+IFNKA  EL++VIGKERWVEEKD+INLPYI+AIAKETMRL
Sbjct: 307 TESSAVTVEWAMSELLKKPEIFNKAREELDRVIGKERWVEEKDIINLPYIDAIAKETMRL 366

Query: 385 HPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAFDPDRFIENSRV 444
           HPV PMLVPRM  EDCQIAGYDI KGTRV VNVWTIGRD TVW+NP  F P+RF+    +
Sbjct: 367 HPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVWENPLEFKPERFM-GKNI 426

Query: 445 DVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDMEKEDLNMEEIFG 504
           DVKGQDFELLPFGSGRRMCPGYSLGLKVI S+LANLLHGF WKL GDM+ EDLNM+E+FG
Sbjct: 427 DVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMDEVFG 486

Query: 505 LSTPKKYPLDAVAEPRLPPHLYSL 528
           LSTPKK+PLD VAEPRL   LY +
Sbjct: 487 LSTPKKFPLDVVAEPRLSSSLYFI 508

BLAST of Cucsat.G12324 vs. ExPASy TrEMBL
Match: A0A5D3C5I8 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G00890 PE=3 SV=1)

HSP 1 Score: 942 bits (2435), Expect = 0.0
Identity = 462/516 (89.53%), Postives = 489/516 (94.77%), Query Frame = 0

Query: 14  MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS 73
           MEM  IPSCLSY+T  I A LALLLLS  LRRRKLNLPPGPKPWP+IGNL LIGSLPHQS
Sbjct: 1   MEMPLIPSCLSYITAWI-AALALLLLSYRLRRRKLNLPPGPKPWPVIGNLHLIGSLPHQS 60

Query: 74  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI 133
           IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKI LKTQDLNFV RPKTAAGKYTTYNYS+I
Sbjct: 61  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDI 120

Query: 134 TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 193
           TWSQYGPYWRQ RKMCLMELFSARRLDSYEYIRKEEMNGL+REIYKSCGEVIKVKDYL  
Sbjct: 121 TWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNGLVREIYKSCGEVIKVKDYLST 180

Query: 194 LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 253
           +SLNVISRMVLGK+YTDESSE+GIVSPDEF+KM+DELFLL+GVLNIGDSIPW+DFLDLQG
Sbjct: 181 VSLNVISRMVLGKKYTDESSENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQG 240

Query: 254 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV 313
           YVKRMK LSKK DRFLEHVLDEH ERRKGV+NYVAKDMVDVLLQLAD P+LEVKLERHGV
Sbjct: 241 YVKRMKALSKKFDRFLEHVLDEHNERRKGVQNYVAKDMVDVLLQLADDPNLEVKLERHGV 300

Query: 314 KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY 373
           KAFTQDL+ GGTE+S +T+EWA+SELLKNP+IFNKA  ELN+VIGKERWVEEKD++NLPY
Sbjct: 301 KAFTQDLIAGGTESSAVTVEWAISELLKNPEIFNKAIEELNEVIGKERWVEEKDIVNLPY 360

Query: 374 INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 433
           INAIAKETMRLHPV PMLVPRMA EDCQIAGYDIAKGTRVLVNVWTIGRD TVW+NPHAF
Sbjct: 361 INAIAKETMRLHPVAPMLVPRMAREDCQIAGYDIAKGTRVLVNVWTIGRDPTVWRNPHAF 420

Query: 434 DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 493
           DPDRF+E+S +DVKGQDFELLPFGSGRRMCPGYSLGLKVI S+LANLLHGFNWKLPGDME
Sbjct: 421 DPDRFMESS-IDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFNWKLPGDME 480

Query: 494 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 529
           KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL
Sbjct: 481 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 514

BLAST of Cucsat.G12324 vs. ExPASy TrEMBL
Match: A0A1S3CKG3 (cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103501983 PE=3 SV=1)

HSP 1 Score: 942 bits (2435), Expect = 0.0
Identity = 462/516 (89.53%), Postives = 489/516 (94.77%), Query Frame = 0

Query: 14  MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS 73
           MEM  IPSCLSY+T  I A LALLLLS  LRRRKLNLPPGPKPWP+IGNL LIGSLPHQS
Sbjct: 1   MEMPLIPSCLSYITAWI-AALALLLLSYRLRRRKLNLPPGPKPWPVIGNLHLIGSLPHQS 60

Query: 74  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI 133
           IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKI LKTQDLNFV RPKTAAGKYTTYNYS+I
Sbjct: 61  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDI 120

Query: 134 TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 193
           TWSQYGPYWRQ RKMCLMELFSARRLDSYEYIRKEEMNGL+REIYKSCGEVIKVKDYL  
Sbjct: 121 TWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNGLVREIYKSCGEVIKVKDYLST 180

Query: 194 LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 253
           +SLNVISRMVLGK+YTDESSE+GIVSPDEF+KM+DELFLL+GVLNIGDSIPW+DFLDLQG
Sbjct: 181 VSLNVISRMVLGKKYTDESSENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQG 240

Query: 254 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV 313
           YVKRMK LSKK DRFLEHVLDEH ERRKGV+NYVAKDMVDVLLQLAD P+LEVKLERHGV
Sbjct: 241 YVKRMKALSKKFDRFLEHVLDEHNERRKGVQNYVAKDMVDVLLQLADDPNLEVKLERHGV 300

Query: 314 KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY 373
           KAFTQDL+ GGTE+S +T+EWA+SELLKNP+IFNKA  ELN+VIGKERWVEEKD++NLPY
Sbjct: 301 KAFTQDLIAGGTESSAVTVEWAISELLKNPEIFNKAIEELNEVIGKERWVEEKDIVNLPY 360

Query: 374 INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 433
           INAIAKETMRLHPV PMLVPRMA EDCQIAGYDIAKGTRVLVNVWTIGRD TVW+NPHAF
Sbjct: 361 INAIAKETMRLHPVAPMLVPRMAREDCQIAGYDIAKGTRVLVNVWTIGRDPTVWRNPHAF 420

Query: 434 DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 493
           DPDRF+E+S +DVKGQDFELLPFGSGRRMCPGYSLGLKVI S+LANLLHGFNWKLPGDME
Sbjct: 421 DPDRFMESS-IDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFNWKLPGDME 480

Query: 494 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 529
           KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL
Sbjct: 481 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYSLL 514

BLAST of Cucsat.G12324 vs. ExPASy TrEMBL
Match: A0A1S3CCA6 (cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103499163 PE=3 SV=1)

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 428/504 (84.92%), Postives = 462/504 (91.67%), Query Frame = 0

Query: 25  YVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQSIHQLSKKYGPI 84
           YV  + IATLA+LLLSR +RRRKLNLPPGPKPWP+IGNLDLIGSLPHQSIHQLSKKYGPI
Sbjct: 7   YVVVAWIATLAILLLSRRIRRRKLNLPPGPKPWPLIGNLDLIGSLPHQSIHQLSKKYGPI 66

Query: 85  MHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNITWSQYGPYWRQ 144
           MHLRFGSFPVVVGSSVEMAKI LKTQDLNFV RPKTAAGKYTTYNYS+ITWSQYGPYWRQ
Sbjct: 67  MHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYWRQ 126

Query: 145 LRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFALSLNVISRMVL 204
            RKMCLMELFSARRLDSYEYIRKEEMN L+REIYKSCGEVIK+KDYL  +SLNVISRMVL
Sbjct: 127 ARKMCLMELFSARRLDSYEYIRKEEMNALLREIYKSCGEVIKIKDYLSTVSLNVISRMVL 186

Query: 205 GKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQGYVKRMKGLSKK 264
           GK+YTDES E+GIVSPDEF+KM+DELFLL+GVLNIGDSIPW+DFLDLQGYVKRMK LSKK
Sbjct: 187 GKKYTDES-ENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKALSKK 246

Query: 265 LDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGVKAFTQDLLGGG 324
            DRFLEHVLDEH ERRKGVE+YVAKDMVDVLLQLAD PDLEVKLERHGVKAFTQDL+ GG
Sbjct: 247 FDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQDLIAGG 306

Query: 325 TETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPYINAIAKETMRL 384
           TE+S +T+EWAMSELLK P+IFNKA  EL++VIGKERWVEEKD+INLPYI+AIAKETMRL
Sbjct: 307 TESSAVTVEWAMSELLKKPEIFNKAREELDRVIGKERWVEEKDIINLPYIDAIAKETMRL 366

Query: 385 HPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAFDPDRFIENSRV 444
           HPV PMLVPRM  EDCQIAGYDI KGTRV VNVWTIGRD TVW+NP  F P+RF+    +
Sbjct: 367 HPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVWENPLEFKPERFM-GKNI 426

Query: 445 DVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDMEKEDLNMEEIFG 504
           DVKGQDFELLPFGSGRRMCPGYSLGLKVI S+LANLLHGF WKL GDM+ EDLNM+E+FG
Sbjct: 427 DVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMDEVFG 486

Query: 505 LSTPKKYPLDAVAEPRLPPHLYSL 528
           LSTPKK+PLD VAEPRL   LY +
Sbjct: 487 LSTPKKFPLDVVAEPRLSSSLYFI 508

BLAST of Cucsat.G12324 vs. ExPASy TrEMBL
Match: A0A5D3D8H9 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1267G00050 PE=3 SV=1)

HSP 1 Score: 875 bits (2261), Expect = 0.0
Identity = 427/504 (84.72%), Postives = 462/504 (91.67%), Query Frame = 0

Query: 25  YVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQSIHQLSKKYGPI 84
           YV  + IATLA+LLLSR +RRRKLNLPPGPKPWP+IGNLDLIGSLPHQSIHQLSKKYGPI
Sbjct: 7   YVVVAWIATLAILLLSRRIRRRKLNLPPGPKPWPLIGNLDLIGSLPHQSIHQLSKKYGPI 66

Query: 85  MHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNITWSQYGPYWRQ 144
           MHLRFGSFPVVVGSSVEMAKI LKTQDLNFV RPKTAAGKYTTYNYS+ITWSQYGPYWRQ
Sbjct: 67  MHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYWRQ 126

Query: 145 LRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFALSLNVISRMVL 204
            RKMCLMELFSARRLDSYEYIRKEEMN L+REIYKSCGEVIK+KDYL  +SLNVISRMVL
Sbjct: 127 ARKMCLMELFSARRLDSYEYIRKEEMNALLREIYKSCGEVIKIKDYLSTVSLNVISRMVL 186

Query: 205 GKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQGYVKRMKGLSKK 264
           GK+YTDES E+GIVSPDEF+KM+DELFLL+GVLNIGDSIPW+DFLDLQGYVKRMK LSKK
Sbjct: 187 GKKYTDES-ENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKALSKK 246

Query: 265 LDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGVKAFTQDLLGGG 324
            DRFLEHVLDEH ERRKGVE+YVAKDMVDVLLQLAD PDLEVKLERHGVKAFTQDL+ GG
Sbjct: 247 FDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVKLERHGVKAFTQDLIAGG 306

Query: 325 TETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPYINAIAKETMRL 384
           TE+S +T+EWAMSELLK P+IFNKA  EL++VIG+ERWVEEKD+INLPYI+AIAKETMRL
Sbjct: 307 TESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKDIINLPYIDAIAKETMRL 366

Query: 385 HPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAFDPDRFIENSRV 444
           HPV PMLVPRM  EDCQIAGYDI KGTRV VNVWTIGRD TVW+NP  F P+RF+    +
Sbjct: 367 HPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVWENPLEFKPERFM-GKNI 426

Query: 445 DVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDMEKEDLNMEEIFG 504
           DVKGQDFELLPFGSGRRMCPGYSLGLKVI S+LANLLHGF WKL GDM+ EDLNM+E+FG
Sbjct: 427 DVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWKLSGDMKNEDLNMDEVFG 486

Query: 505 LSTPKKYPLDAVAEPRLPPHLYSL 528
           LSTPKK+PLD VAEPRL   LY +
Sbjct: 487 LSTPKKFPLDVVAEPRLSSSLYFI 508

BLAST of Cucsat.G12324 vs. ExPASy TrEMBL
Match: A0A6J1GC60 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111452799 PE=3 SV=1)

HSP 1 Score: 865 bits (2236), Expect = 2.91e-314
Identity = 428/514 (83.27%), Postives = 466/514 (90.66%), Query Frame = 0

Query: 14  MEMQPIPSCLSYVTTSIIATLALLLLSRCLRRRKLNLPPGPKPWPIIGNLDLIGSLPHQS 73
           M++Q IPS + Y    + ATLA+LLLSR LRR  LN PPGPKPWP IGNL LIGSLPHQS
Sbjct: 1   MDIQ-IPSWVFYAAAWM-ATLAILLLSRRLRRPNLNPPPGPKPWPFIGNLHLIGSLPHQS 60

Query: 74  IHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIVLKTQDLNFVWRPKTAAGKYTTYNYSNI 133
           IHQLSKKYGPIM LRFGSFPVVVGSSVEMAKI LKT DLNFV RPKTAAGKYTTYNYS+I
Sbjct: 61  IHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDI 120

Query: 134 TWSQYGPYWRQLRKMCLMELFSARRLDSYEYIRKEEMNGLIREIYKSCGEVIKVKDYLFA 193
           TWSQYGPYWRQ RKMCL+ELFSARRLDSYEYIR+EEM GLI+EIYKSCGE I++KDYL  
Sbjct: 121 TWSQYGPYWRQARKMCLLELFSARRLDSYEYIRREEMKGLIKEIYKSCGEGIQLKDYLST 180

Query: 194 LSLNVISRMVLGKRYTDESSESGIVSPDEFRKMMDELFLLNGVLNIGDSIPWMDFLDLQG 253
           LSLNVISRMVLGK+YT+ES E+GIVSPDEF+KM+DELFLL+GVLNIGDSIPW+DFLDLQG
Sbjct: 181 LSLNVISRMVLGKKYTEES-ENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQG 240

Query: 254 YVKRMKGLSKKLDRFLEHVLDEHKERRKGVENYVAKDMVDVLLQLADHPDLEVKLERHGV 313
           YVKRMK LSKK DRFLEHVLDEH ERRKGVE YVAKDMVDVLLQLAD P+LEVKLERHGV
Sbjct: 241 YVKRMKALSKKFDRFLEHVLDEHNERRKGVEGYVAKDMVDVLLQLADDPNLEVKLERHGV 300

Query: 314 KAFTQDLLGGGTETSTITIEWAMSELLKNPKIFNKATIELNKVIGKERWVEEKDMINLPY 373
           KAFTQDL+ GGTE+S +T+EWAMSELLKNP+IF KA  EL++VIG+ RWVEE+DM+NLPY
Sbjct: 301 KAFTQDLIAGGTESSAVTVEWAMSELLKNPEIFGKAREELDRVIGRGRWVEERDMVNLPY 360

Query: 374 INAIAKETMRLHPVVPMLVPRMAGEDCQIAGYDIAKGTRVLVNVWTIGRDQTVWKNPHAF 433
           INAIAKETMRLHPV PMLVPRM  EDC+IAGYDI KGTRVLVNVWTIGRD  VWKN HAF
Sbjct: 361 INAIAKETMRLHPVAPMLVPRMCREDCRIAGYDIVKGTRVLVNVWTIGRDSEVWKNAHAF 420

Query: 434 DPDRFIENSRVDVKGQDFELLPFGSGRRMCPGYSLGLKVILSTLANLLHGFNWKLPGDME 493
           DP+RF+ +S V+VKGQDFELLPFGSGRRMCPGYSLGLKVI S+LANLLHGF WKLPGDME
Sbjct: 421 DPERFVGSS-VEVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFCWKLPGDME 480

Query: 494 KEDLNMEEIFGLSTPKKYPLDAVAEPRLPPHLYS 527
           KEDLN+EE+FGLSTPKK+PL AVA PRLPPHLYS
Sbjct: 481 KEDLNLEEVFGLSTPKKFPLQAVAHPRLPPHLYS 510