Cucsat.G12220 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G12220
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	tropinone reductase homolog
Location	ctg1837: 2401412 .. 2405395 (-)
RNA-Seq Expression	Cucsat.G12220
Synteny	Cucsat.G12220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTCAACTAGAACATTGTATTAAAAACTAAAAAATGATTGTCAAATTAAAATTTGAATGTAGACATAATAATTACTAAGCTGAATTGTTTATATATTTATATGTTATCCTAAGTTGATGAATTATAAAAGAAAAAATGAATCTCGTGGTTCGGACCTCACCATAATAATTGAATATTTACATATTAAAATGATGCAATTTTTAGTTCATATAATTAAGTTAAGAAAAATCGAGCGAGAGTGATCTCTCTCTTCCTTCATTTGTTGTGAATCAAATCGAAAGTCGAAACCCAACCCAGCTGCCATGGCCAACATTAGAAACCAAAGATGGTCACTCAAGGGGATGACCGCTCTCGTCACCGGCGGAAGCCGCGGCATCGGGTACTCCTAATTTTCAGTCTTTATTTTCGTTACGCCGATTCATCGAACTCCTGTAATGTTTTTTCTTTCTTCAGACGAGCCACGGTGGAAGAACTTGCAGAATTCGGGGCCAGGGTTCACACATGCTGTCGAAGCCAAGAAGATTTAGACAAATGTTTGAAAGAATGGGAAGCAATGGGCTTCAAAGTGAGTGGCTCTGTATGCGATGTCCAAAGCAAAGAACAGAGGAAGAAGCTGATGGAAACCGTTTCCTCACTCTTCAATGGGACCCTAAACATTCTGGTAATTAACTCCCAAGAAAAAAAAAACGAAAGTTTTGATTTTTGGATTTTGGATTTTGGATTTTGGATTTTGATCGATTATTTGAGTTAGGTGAATAATGCTGGGAGAACGTTGTCGTCGTTGAAGTCGACGGTAGAAGTAACAGAGGAAGATATTTCAAGTGTAATGAGTACAAATTTTGAGTCGTCGTTCCATTTTAGCCAACTTGCATATCCATTACTAAAGGCTTCTGGTAATGGAAGCATTGTGTTCATATCCTCTGTTTCAGGCTTAACGGCCCTTCCTTTCTCTACTCCATATGCTGCTTCTAAAGGTAACCCACAAATTTCTTCACCTTTACCCACCTTATTTTGAATTTGAATTTGTGTTCTTATAACATGAATTTGTGTTCTTATAACATGAATTTGTTGATGAAGCTGCCATAAATCAAATCACAAAGAACTTGGCATGTGAATGGGCAAAAGATAACATCCGTACAAACGCAGTGGCTCCATGGATCATCAAAACTCGCCTTGTCGAGCGTTCCAATGTACAAACTTTCATTCATTATCGATATCTCTTTCAATTCGATCCAAGTTTATTAATTATTTTTTTAACTTTTTTATTATTTTTCAGGATGATCCAATGCATGTAAAAGGAATAGAGCAACTATTGTCAGTAACGCCTCTTAAACGGGCAGGGGAACCACATGAAGTTTCGTCAATGGTTGTTTTCCTTTGTCTTCCAGCTGCGTCTTATATTACTGGACAACTTTTTGTTATTGATGGAGGCCACACTGTCAAGGCTTACCCCATACCCGATCTCTGAAACTAAACAAAACTAGTCTTTGTATTTCTCTATGTAATTTTAATCAATTTTAAAATTAGTATGGACTTGAATCTTTAGACTATATACAATCAATCTAATTTCCAATCACTCATCGTACACCAAGGGATCTGTAGGTTTAATTCCAAAACGGTAGTTTGATCAATCTCTGAAACTTGTGAATCTATCTTTCTCAACAATTCAATAAGCAGAGAAAA

Coding sequence (CDS)

ATGAGTACAAATTTTGAGTCGTCGTTCCATTTTAGCCAACTTGCATATCCATTACTAAAGGCTTCTGGTAATGGAAGCATTGTGTTCATATCCTCTGTTTCAGGCTTAACGGCCCTTCCTTTCTCTACTCCATATGCTGCTTCTAAAGCTGCCATAAATCAAATCACAAAGAACTTGGCATGTGAATGGGCAAAAGATAACATCCGTACAAACGCAGTGGCTCCATGGATCATCAAAACTCGCCTTGTCGAGCGTTCCAATGATGATCCAATGCATGTAAAAGGAATAGAGCAACTATTGTCAGTAACGCCTCTTAAACGGGCAGGGGAACCACATGAAGTTTCGTCAATGGTTGTTTTCCTTTGTCTTCCAGCTGCGTCTTATATTACTGGACAACTTTTTGTTATTGATGGAGGCCACACTGTCAAGGCTTACCCCATACCCGATCTCTGA

Protein sequence

MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAASYITGQLFVIDGGHTVKAYPIPDL

Homology

[Close]

BLAST of Cucsat.G12220 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.1e-89
Identity = 164/260 (63.08%), Postives = 200/260 (76.92%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVS 65
           ++RWSL+GMTALVTGG+RGIG A VEELA FGA V+TC RSQ DLD+CL++W   GFKVS
Sbjct: 10  DRRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKVS 69

Query: 66  GSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNF 125
           G VCDV S  QR+ LME+V+S FNG LNIL+NNAG T+   K     T ED S +M TNF
Sbjct: 70  GPVCDVSSISQRQTLMESVTSSFNGKLNILINNAGTTIP--KEATNFTAEDYSIIMGTNF 129

Query: 126 ESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAK 185
           E+S++  QLA+PLLKASGN SIVF SS +G+ A+P S+ YAASK AINQ+TK+LACEWAK
Sbjct: 130 EASYNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKSLACEWAK 189

Query: 186 DNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPA 245
           D+IR NAVAPWII T ++E +   P   K IE L+   P+KRAGEP EVSS+V +LCLP 
Sbjct: 190 DSIRVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTYLCLPT 249

Query: 246 ASYITGQLFVIDGGHTVKAY 266
           ASYITGQ+  +DGG+TV  +
Sbjct: 250 ASYITGQIICVDGGYTVNGF 267

[Close]

BLAST of Cucsat.G12220 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.1e-83
Identity = 157/260 (60.38%), Postives = 198/260 (76.15%), Query Frame = 0


Query: 6   NQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVS 65
           ++RWSL G TALVTGG+RGIGRA VEELA+FGA+VHTC R+QE+L+ CL +W+A G  VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 66  GSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNF 125
           GSVCD   ++QR+KL++  SS F+G LNIL+NN G  +   K TVE + E+ + +MSTN 
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVR--KPTVEYSSEEYAKIMSTNL 123

Query: 126 ESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAK 185
           ES+FH SQ+A+PLLKASG GSIVFISSV+GL  L   + Y A+K A+NQ+T+NLACEWA 
Sbjct: 124 ESAFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWAS 183

Query: 186 DNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPA 245
           DNIRTN VAPW IKT LVE   +     + +E ++S TPL R GEP EVSS+V FLCLPA
Sbjct: 184 DNIRTNCVAPWYIKTSLVETLLEKK---EFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPA 243

Query: 246 ASYITGQLFVIDGGHTVKAY 266
           +SYITGQ+  +DGG TV  +
Sbjct: 244 SSYITGQVISVDGGFTVNGF 258

[Close]

BLAST of Cucsat.G12220 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.5e-80
Identity = 152/259 (58.69%), Postives = 193/259 (74.52%), Query Frame = 0


Query: 7   QRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSG 66
           +RWSL GM+ALVTGG+RGIGRA VEELA  GA VHTC R++ +L+ CL +W   GF+V+G
Sbjct: 64  ERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAG 123

Query: 67  SVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFE 126
           SVCDV  + QR+ LMETVSS+F G L+ILVNN G  +   K  VE T  + S++MSTNFE
Sbjct: 124 SVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIR--KPMVEFTAGEFSTLMSTNFE 183

Query: 127 SSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKD 186
           S FH  QLAYPLL+ S  GS+VFISSVSG  +L   +  +++K AINQ+T++LACEWAKD
Sbjct: 184 SVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKD 243

Query: 187 NIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAA 246
           NIR NAVAPW IKT +VE+   +  +   +E++ SVTPL R GEP EVSS V FLCLPA+
Sbjct: 244 NIRINAVAPWYIKTSMVEQVLSNKEY---LEEVYSVTPLGRLGEPREVSSAVAFLCLPAS 303

Query: 247 SYITGQLFVIDGGHTVKAY 266
           SYITGQ+  +DGG ++  +
Sbjct: 304 SYITGQILCVDGGMSINGF 317

[Close]

BLAST of Cucsat.G12220 vs. ExPASy Swiss-Prot
Match: Q9ZW20 (Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g29370 PE=3 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.7e-79
Identity = 152/262 (58.02%), Postives = 197/262 (75.19%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSGS 67
           +WSL+GMTALVTGGS+G+G+A VEELA  GARVHTC R +  L + L+EW+A G +V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 68  VCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFES 127
           VCDV S++QR+KLMETVSSLF G L+ILV N G  +  LK T E T E+ S +++TN ES
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVG--IGVLKPTTECTAEEFSFIIATNLES 132

Query: 128 SFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDN 187
           +FHFSQLA+PLLKASG+G+IV +SSV+G+  L  ++ Y A+K A+NQ+ +NLACEWA DN
Sbjct: 133 TFHFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDN 192

Query: 188 IRTNAVAPWIIKTRLVERSNDDPMHVKGI-EQLLSVTPLKRAGEPHEVSSMVVFLCLPAA 247
           IR N+V PW I T     S  D +  K + E++ SVTPL+R GE +EVSS+V FLCLPAA
Sbjct: 193 IRANSVCPWFITT----PSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAA 252

Query: 248 SYITGQLFVIDGGHTVKAYPIP 269
           SYITGQ   +DGG T+  + +P
Sbjct: 253 SYITGQTICVDGGFTINGFSLP 268

[Close]

BLAST of Cucsat.G12220 vs. ExPASy Swiss-Prot
Match: Q9ZW19 (Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 3.8e-79
Identity = 154/264 (58.33%), Postives = 194/264 (73.48%), Query Frame = 0


Query: 8   RWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAMGFKVSGS 67
           RWSL GMTALVTGGS+GIG A VEELA  GAR+HTC R +  L + L++W+A GF+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 68  VCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSVMSTNFES 127
           VCDV S+++R+KLMETVS++F G LNILVNN G  +  +K T++ T ED S  M+TN ES
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCI--VKPTLQHTAEDFSFTMATNLES 132

Query: 128 SFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLACEWAKDN 187
           +FH SQLA+PLLKASG+GSIV ISSVSG+  +  ++ Y  SK A+NQ+ +NLACEWA DN
Sbjct: 133 AFHLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDN 192

Query: 188 IRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVFLCLPAAS 247
           IRTN+V PW I+T LV  S  +    K +E   S  P+ R GE +EVSS+V FLCLPAAS
Sbjct: 193 IRTNSVCPWFIETPLVTESLSNEEFRKEVE---SRPPMGRVGEVNEVSSLVAFLCLPAAS 252

Query: 248 YITGQLFVIDGGHTVKAY---PIP 269
           YITGQ   +DGG TV  +   P+P
Sbjct: 253 YITGQTICVDGGFTVNGFSFKPLP 271

[Close]

BLAST of Cucsat.G12220 vs. NCBI nr
Match: XP_004137777.1 (tropinone reductase homolog [Cucumis sativus] >KGN58842.1 hypothetical protein Csa_002580 [Cucumis sativus])

HSP 1 Score: 532 bits (1370), Expect = 2.04e-190
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM
Sbjct: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV
Sbjct: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA
Sbjct: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKAYPIPDL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270

[Close]

BLAST of Cucsat.G12220 vs. NCBI nr
Match: XP_008442511.1 (PREDICTED: tropinone reductase homolog [Cucumis melo] >KAA0044114.1 tropinone reductase-like protein [Cucumis melo var. makuwa] >TYK25024.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 507 bits (1306), Expect = 1.16e-180
Identity = 256/270 (94.81%), Postives = 262/270 (97.04%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKG TALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWE M
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GF+VSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEE+ISSV
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFE+S HFSQLAYPLLKASGNGSIVFISSVSGL ALPFSTPYAASKAAINQ+TKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTR VE SNDDPMHVKG+EQ+LSVTPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKAYPI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of Cucsat.G12220 vs. NCBI nr
Match: XP_038904663.1 (tropinone reductase homolog [Benincasa hispida])

HSP 1 Score: 467 bits (1201), Expect = 1.12e-164
Identity = 237/270 (87.78%), Postives = 251/270 (92.96%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MAN RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGA VHTCCRSQE+LDKCLKEWE M
Sbjct: 1   MANNRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGATVHTCCRSQENLDKCLKEWEEM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           G KVSGSVCDVQS+EQRKKLMETVSSLFNG+LNILVNNAG T S LK+  EVTEE+I SV
Sbjct: 61  GLKVSGSVCDVQSREQRKKLMETVSSLFNGSLNILVNNAGTT-SELKTAAEVTEEEIWSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           M+TNFE+SFH SQLAYPLLKASGNG+IVFISSVSGL ALP+STPYAASKAAINQ+TKNLA
Sbjct: 121 MNTNFEASFHSSQLAYPLLKASGNGNIVFISSVSGLMALPYSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIR NAVAPWII+TR VE  NDDP++VK +EQLLSVTPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRVNAVAPWIIRTRFVEPPNDDPIYVKSMEQLLSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKAYPI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 269

[Close]

BLAST of Cucsat.G12220 vs. NCBI nr
Match: XP_022971600.1 (tropinone reductase homolog [Cucurbita maxima])

HSP 1 Score: 431 bits (1108), Expect = 1.65e-150
Identity = 217/270 (80.37%), Postives = 244/270 (90.37%), Query Frame = 0


Query: 1   MANI-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEA 60
           MAN  R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA VHTC RSQ DLDKCLKEWE 
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISS 120
           MG  VSGSVCDV S+EQRK+LMETVSSLFNG+LNILVNNAG   S+ KS++EVTEE++SS
Sbjct: 61  MGLNVSGSVCDVHSREQRKRLMETVSSLFNGSLNILVNNAGT--SAYKSSLEVTEEELSS 120

Query: 121 VMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNL 180
           VMSTNFE+SFHFSQLA+PL+KASGNGSIVF+SSV+GL +LP+STPYAASKAAINQIT+NL
Sbjct: 121 VMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNL 180

Query: 181 ACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVV 240
           ACEWAKDNIRTNAVAPWII+TR V+   DDP+HV+ +  +LSVTPLKRAGEPHEVSSMV 
Sbjct: 181 ACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 240

Query: 241 FLCLPAASYITGQLFVIDGGHTVKAYPIPD 269
           FLCLPAASYITGQ+F IDGGHTV A+PIPD
Sbjct: 241 FLCLPAASYITGQVFCIDGGHTVTAFPIPD 268

[Close]

BLAST of Cucsat.G12220 vs. NCBI nr
Match: XP_022941371.1 (tropinone reductase homolog [Cucurbita moschata])

HSP 1 Score: 431 bits (1107), Expect = 2.35e-150
Identity = 215/270 (79.63%), Postives = 245/270 (90.74%), Query Frame = 0


Query: 1   MANI-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEA 60
           MAN  R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA VHTC RSQ DLDKCLKEWE 
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISS 120
           MGF VSGSVCDV S+EQR +L+ETVSSLFNG+LNILVNNAG   S+ KS++EVTEE++SS
Sbjct: 61  MGFNVSGSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAGT--SAYKSSLEVTEEELSS 120

Query: 121 VMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNL 180
           VMSTNFE++FHFSQLA+PL+KASGNGSIVF+SSV+GL +LP+STPYAASKAAINQIT+NL
Sbjct: 121 VMSTNFEAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNL 180

Query: 181 ACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVV 240
           ACEWAKDNIRTNAVAPWII+TR V+   DDP+HV+ +  +LSVTPLKRAGEPHEVSSMV 
Sbjct: 181 ACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 240

Query: 241 FLCLPAASYITGQLFVIDGGHTVKAYPIPD 269
           FLCLPAASYITGQ+F IDGGHTVKA+P+PD
Sbjct: 241 FLCLPAASYITGQVFCIDGGHTVKAFPVPD 268

[Close]

BLAST of Cucsat.G12220 vs. ExPASy TrEMBL
Match: A0A0A0LDM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733960 PE=4 SV=1)

HSP 1 Score: 532 bits (1370), Expect = 9.86e-191
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM
Sbjct: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV
Sbjct: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA
Sbjct: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKAYPIPDL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270

[Close]

BLAST of Cucsat.G12220 vs. ExPASy TrEMBL
Match: A0A5D3DN54 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001430 PE=4 SV=1)

HSP 1 Score: 507 bits (1306), Expect = 5.63e-181
Identity = 256/270 (94.81%), Postives = 262/270 (97.04%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKG TALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWE M
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GF+VSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEE+ISSV
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFE+S HFSQLAYPLLKASGNGSIVFISSVSGL ALPFSTPYAASKAAINQ+TKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTR VE SNDDPMHVKG+EQ+LSVTPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKAYPI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of Cucsat.G12220 vs. ExPASy TrEMBL
Match: A0A1S3B5V0 (tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486361 PE=4 SV=1)

HSP 1 Score: 507 bits (1306), Expect = 5.63e-181
Identity = 256/270 (94.81%), Postives = 262/270 (97.04%), Query Frame = 0


Query: 1   MANIRNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEAM 60
           MANIRNQRWSLKG TALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWE M
Sbjct: 1   MANIRNQRWSLKGKTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEEM 60

Query: 61  GFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISSV 120
           GF+VSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEE+ISSV
Sbjct: 61  GFEVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEEISSV 120

Query: 121 MSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNLA 180
           MSTNFE+S HFSQLAYPLLKASGNGSIVFISSVSGL ALPFSTPYAASKAAINQ+TKNLA
Sbjct: 121 MSTNFEASLHFSQLAYPLLKASGNGSIVFISSVSGLMALPFSTPYAASKAAINQMTKNLA 180

Query: 181 CEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVVF 240
           CEWAKDNIRTNAVAPWIIKTR VE SNDDPMHVKG+EQ+LSVTPLKRAGEPHEVSSMV F
Sbjct: 181 CEWAKDNIRTNAVAPWIIKTRFVESSNDDPMHVKGMEQILSVTPLKRAGEPHEVSSMVAF 240

Query: 241 LCLPAASYITGQLFVIDGGHTVKAYPIPDL 270
           LCLPAASYITGQLFVIDGGHTVKAYPI DL
Sbjct: 241 LCLPAASYITGQLFVIDGGHTVKAYPISDL 270

[Close]

BLAST of Cucsat.G12220 vs. ExPASy TrEMBL
Match: A0A6J1I667 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=1)

HSP 1 Score: 431 bits (1108), Expect = 8.00e-151
Identity = 217/270 (80.37%), Postives = 244/270 (90.37%), Query Frame = 0


Query: 1   MANI-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEA 60
           MAN  R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA VHTC RSQ DLDKCLKEWE 
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISS 120
           MG  VSGSVCDV S+EQRK+LMETVSSLFNG+LNILVNNAG   S+ KS++EVTEE++SS
Sbjct: 61  MGLNVSGSVCDVHSREQRKRLMETVSSLFNGSLNILVNNAGT--SAYKSSLEVTEEELSS 120

Query: 121 VMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNL 180
           VMSTNFE+SFHFSQLA+PL+KASGNGSIVF+SSV+GL +LP+STPYAASKAAINQIT+NL
Sbjct: 121 VMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNL 180

Query: 181 ACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVV 240
           ACEWAKDNIRTNAVAPWII+TR V+   DDP+HV+ +  +LSVTPLKRAGEPHEVSSMV 
Sbjct: 181 ACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 240

Query: 241 FLCLPAASYITGQLFVIDGGHTVKAYPIPD 269
           FLCLPAASYITGQ+F IDGGHTV A+PIPD
Sbjct: 241 FLCLPAASYITGQVFCIDGGHTVTAFPIPD 268

[Close]

BLAST of Cucsat.G12220 vs. ExPASy TrEMBL
Match: A0A6J1FKX7 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 SV=1)

HSP 1 Score: 431 bits (1107), Expect = 1.14e-150
Identity = 215/270 (79.63%), Postives = 245/270 (90.74%), Query Frame = 0


Query: 1   MANI-RNQRWSLKGMTALVTGGSRGIGRATVEELAEFGARVHTCCRSQEDLDKCLKEWEA 60
           MAN  R+QRWSL+GMTALVTGG+ GIGRATVEELA FGA VHTC RSQ DLDKCLKEWE 
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFKVSGSVCDVQSKEQRKKLMETVSSLFNGTLNILVNNAGRTLSSLKSTVEVTEEDISS 120
           MGF VSGSVCDV S+EQR +L+ETVSSLFNG+LNILVNNAG   S+ KS++EVTEE++SS
Sbjct: 61  MGFNVSGSVCDVHSREQRMQLIETVSSLFNGSLNILVNNAGT--SAYKSSLEVTEEELSS 120

Query: 121 VMSTNFESSFHFSQLAYPLLKASGNGSIVFISSVSGLTALPFSTPYAASKAAINQITKNL 180
           VMSTNFE++FHFSQLA+PL+KASGNGSIVF+SSV+GL +LP+STPYAASKAAINQIT+NL
Sbjct: 121 VMSTNFEAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQITRNL 180

Query: 181 ACEWAKDNIRTNAVAPWIIKTRLVERSNDDPMHVKGIEQLLSVTPLKRAGEPHEVSSMVV 240
           ACEWAKDNIRTNAVAPWII+TR V+   DDP+HV+ +  +LSVTPLKRAGEPHEVSSMV 
Sbjct: 181 ACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 240

Query: 241 FLCLPAASYITGQLFVIDGGHTVKAYPIPD 269
           FLCLPAASYITGQ+F IDGGHTVKA+P+PD
Sbjct: 241 FLCLPAASYITGQVFCIDGGHTVKAFPVPD 268

The following BLAST results are available for this feature:

BLAST of Cucsat.G12220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50165	3.1e-89	63.08	Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
Q9LHT0	1.1e-83	60.38	Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060...	[more]
Q9ZW12	1.5e-80	58.69	Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=...	[more]
Q9ZW20	1.7e-79	58.02	Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g293...	[more]
Q9ZW19	3.8e-79	58.33	Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=...	[more]

BLAST of Cucsat.G12220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137777.1	2.04e-190	100.00	tropinone reductase homolog [Cucumis sativus] >KGN58842.1 hypothetical protein C...	[more]
XP_008442511.1	1.16e-180	94.81	PREDICTED: tropinone reductase homolog [Cucumis melo] >KAA0044114.1 tropinone re...	[more]
XP_038904663.1	1.12e-164	87.78	tropinone reductase homolog [Benincasa hispida]	[more]
XP_022971600.1	1.65e-150	80.37	tropinone reductase homolog [Cucurbita maxima]	[more]
XP_022941371.1	2.35e-150	79.63	tropinone reductase homolog [Cucurbita moschata]	[more]

BLAST of Cucsat.G12220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDM3	9.86e-191	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733960 PE=4 SV=1	[more]
A0A5D3DN54	5.63e-181	94.81	Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B5V0	5.63e-181	94.81	tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486361 PE=4 SV=1	[more]
A0A6J1I667	8.00e-151	80.37	tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=...	[more]
A0A6J1FKX7	1.14e-150	79.63	tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 19..35 score: 35.35 coord: 66..83 score: 37.23 coord: 45..64 score: 42.77 coord: 104..124 score: 33.84
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 45..64 score: 40.37 coord: 25..33 score: 53.59
None	No IPR available	GENE3D	3.40.50.720		coord: 1..145 e-value: 8.5E-47 score: 161.7
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 1..141 e-value: 6.6E-37 score: 127.3
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 11..144 e-value: 2.7E-11 score: 40.9
None	No IPR available	PANTHER	PTHR42898:SF28	TROPINONE REDUCTASE-LIKE PROTEIN	coord: 1..144
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 1..144
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 32..60
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 1..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G12220.T8	Cucsat.G12220.T8	mRNA
Cucsat.G12220.T1	Cucsat.G12220.T1	mRNA
Cucsat.G12220.T3	Cucsat.G12220.T3	mRNA
Cucsat.G12220.T4	Cucsat.G12220.T4	mRNA
Cucsat.G12220.T5	Cucsat.G12220.T5	mRNA
Cucsat.G12220.T2	Cucsat.G12220.T2	mRNA
Cucsat.G12220.T6	Cucsat.G12220.T6	mRNA
Cucsat.G12220.T7	Cucsat.G12220.T7	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity