Cucsat.G12141 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12141
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionCytochrome P450 705A5-like protein
Locationctg1837: 989589 .. 993536 (-)
RNA-Seq ExpressionCucsat.G12141
SyntenyCucsat.G12141
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTTATTTTAAAACTTGAACGTGACACGAACAATATTTTAATTCCAAAGTAAAGAATTATATGAAAGACTTGGCTGCAAGCAGGAAGATGAAGAAGCTCCGTTTTCCGAGTGGAATCTAAAATGGCCGATTCAACCTCTTACCTTCTCTACATTTTTCTCTTCTTTTTCTTCACCATTCTTCTTCATTATCTCTTTCATCGACGTTCTTATGCCCCCCGCCGTCCCCCCACTCCCCCGGCCCTTCCGCTAATCGGCCACCTCCACCACTTCTCGCCATCCGTTTATAAATCCTTCCACAATCTCGCCGCCAAGTACGGCGATCTTCTCTTTCTCCGACTCGGCAGAGTCCCATGCGTCGTCATTTCCTCGGCGTCCTACGCCGCCGAAATCTACAAGAATCAGGACGTTAATTTCTCGTCGCGGCCGAAATTCGCGTTCGGCGATGAGCTTCCGTACGCGAATGCAGGATTCTTCGCGGCAGAGTACGGGGATTATTGGAGATTCATGAAGAAACTGACGATGACGGAGCTTTTATCGCAGCGGCAAGTTGAGAGATCGCGCGGTGTCCGGAGAGAAGAAATGTTGAAATTGTTGAGGAAATTGTGTGAGTGCGGCGAGAAGAAGGAGGCTGTGGATTTAGGGGCGGAGCTCGTCAAACTCACCAACAACAGCACTTGTAGGTATGAAATCATAAACCAATCATTTTTCATCTCAACGTATATTCAACTTTTCTTTGATCGTAACCAAAGTTAGAATTTAGAAATTGATTTCATAAGTCTATGATTTAACTCTTAACTCAATGTTGATCGACGGACTATAATCTAACCACAAACTTTCTAACACGCTCCAAAACTCAACTGAAGTGTCTCATATAAAATCGCATAAAAACTCTATATTGTAGTTTGATTAAAAAAATTATCACCATGTTAGAACTATAAGTCATAGCACTCTTGCTTTTTCAAAACAAGGATGTAAATTTGTAATTTAACCTAAACAAAGTTTATTTTTAATTAGGATATCATACCTGAAGATTAAGAAATCAAATTAATTAAATTAAATGATTCAAAATCATTGATTAAGAAATCAAATTAACTAAATTAAATGATTCAAAATCATTGACAAACATATAGCTCGACACATGCCTTCTATTTGAGAAAAAAAGCCGTAAGACGAAAATTATATAATCCATCCCCTAGTTGTTTTAGGAAAAGTTTGCTTCTTCTAACTATGTACTAAGAAATATTGTGTGAGACATTTTAGTTTATATTCTAAAAAGCTAACCTTATTTAAATCTTATTACTTGTTTAAAATGTATCTTCTATTTAAAAAAAGAGATTAGAGCAATCGAAGCTAAGTAAAAGTAAATAGAGATTGAAAAGAAGATTATAATATACTTGAAATTTCATAAAATTAAAACTCATTTCGGTTTATCTAATTAATTTATTGGAGTCAATGGGGGATTTCAACTTGCATTTATTTATACTCTATATCAAATATAATAACAGTATCTTAAAATACAATTTTACAACTATGTAGAATGCAATATAAAATTTTATATTAAGCTTGAATCAATTAAATATTTAACTAGGTAGATTACAAAAATAAACTAAATGATTTTTCTAAAAAATTGTATTATACAAATATATGACAACGATGTTCATGTAAAATAAAATATGACCTAACCATATACCATAATACCAACCAAATTAAATATTTTTGTTCCACTATATCTACATTAGTATTAACCTATAAAATAAGTGACTAAACATTAGAGTAAAAATATGGAGACTGTTGATTAACATTTTAAAAAGATTTTTCTTGACAAATTATTGGTCTTTTCTAATTGTTAACTGCCCGCCATGCAATTGTTTTCATTCTCACAATTTCATTAAGACTTGTTAATAAGAATAGAAACATATTTAATTATTTACCACAATACTCAAATTTCCAAATTTAATGGAATTTAATATGGTGAATGAACTTCTCCCATTTCTATTTAAATTATAGCATATAAAATAAAGACATAACTTTCTTTTTCTTTAGATTTTTTTACAAGAGCTTCTCATTTTATTAAAACTTACAAGATCATTAAACTTGTTTATAATTTAAAAAATTAGAAAAATAATAATTAATTAGAAAAATGTCTTAATTTTTATCAAGCACAAATACACTCACTTTCATTATTTTTAGTACAAATTTAGGGTAGAAAATTTTGAATCAAAGTACTTTTTTATCTTTAAATACATGTATGTGCTAGTTGAGTAAAGTTTTTGTTGACACACATTTTCATTATCTTTTTTTTAAAGAAATATATTTATTTAACTTGTATCTTAGAGTAATTGCGACAAATAGAAAGTTTAGGACTAATAATTAAGAGTATATAAATATTTTTAAATAATTATAAATACAACAAAATTTAAAATTCATTTATACTTGGCTATGTTTACTAATAGTTTAAATGTGTCCACAGCCATTATTTTGTAAAACTAATATCAATGATTTGGATTGAGCAATAGGTTGGTGATGAGTACGAGATGTTCAGGTGATGATGATGAAGCGGAGAAGATAAGATTATTAGTAAAAGAAACATTTGAGATGGCTTCAAAAGTAGCATTTGGGGATGTGTTTGGATGGCCTTTAGAGCGGCTGGCGTTTTGGATGTTTGGAAGACAAGCAAGGGATGTGACTTTGAGGTATGATGAGATTTTGGAGAAAATATTAAGGCAACATGAAGATAGAGGAAAAAGAGAAGGTTTGGATAGGGAAGATAGAGATTTAATGGACATTTTGTTGAAGGTTTATCAAGATCACAATGCTGAGTTCAACATTACTAGAACCAATATCAAGGCTTTCTTGCTTGTAAGTCTTCACCCCCTTCTTATCATATATTTAATTACCAACTTTTCCCTTCATTTATATTTCATGATTATACATCTCTATAATCTTTAATAGGTTTGAAAGTGATGATTTAAGAAATTTAATTATCAAATTTAAGTGGATGACCTAAACATTTTAGAGTTGGATGATAGTATGAAATTTCATGAAATTCATTGTGTAGGATTTATTTCTAGGCGGAACTGGTACATCAACAGAGGTATCACAGTGGACAATGGCGGAACTTCTGAACCATCCTAATGTATTCAACAAACTTAGAAACGAAATAAACTATGTCGTAGGAACCACCAGACTCGTCGGAGAAGACGACCTCCCAAATCTCCCTTACTTACAAGCTATCATGAAGGAGGCTCTCCGCCTCTACCCTGCTGTCCCAATTGCCATGAGAGTATGTCGTCAAGACTGCATAATCGACGGCTACGATATCCCCAAGGACACAATGGTTGCAGTCAACCTGTTCGACATAATGCGCGACCCAAAAATCTGGGAAAATCCAAACGAATTCGACCCCGAAAGATTCACTGGCGATGTGAAGTACGAGATTAAGGGGCAACAAAGTTTCAATTTTGTGCCCTTTGGTGGGGGAAGACGAGCTTGCCCTGGCTCCACTTTGGCCTTCAGTTTCATTAGCAATGTGATTGCAACAATGGTTCAGTGTTTTGATTGGAAAATTATTGGAAGACCAGATAATAATGAAGATGGAATAAGTAAAGTGGATATGGAGATTGGAGTTGCCTTTACTTTGCCCATGGCTAACCCTCTTCGTTGTGTTCCTATGGTTCGCTTCAACCCTTTTAATGACACTCTAAATGAATGAGGAAGCACTTTGGATGAGGATATGGAAATTGGGGTGTAGTTGGGCTATATAGACTTTACATTATTAATATCAACCAAGAGACAATTTAATTGTCATAATGACAATGCAAATTTACATTTAAAGTAGATTTTGATGGAATAAGCTAAGTACCAACTAACTTGAACTATGGTAAAAATGTTTATTATTCATCCCACATCTCATAGAAAAAAGTATATATAATAACTCAAAAGGGTAA

Coding sequence (CDS)

ATGGCCGATTCAACCTCTTACCTTCTCTACATTTTTCTCTTCTTTTTCTTCACCATTCTTCTTCATTATCTCTTTCATCGACGTTCTTATGCCCCCCGCCGTCCCCCCACTCCCCCGGCCCTTCCGCTAATCGGCCACCTCCACCACTTCTCGCCATCCGTTTATAAATCCTTCCACAATCTCGCCGCCAAGTACGGCGATCTTCTCTTTCTCCGACTCGGCAGAGTCCCATGCGTCGTCATTTCCTCGGCGTCCTACGCCGCCGAAATCTACAAGAATCAGGACGTTAATTTCTCGTCGCGGCCGAAATTCGCGTTCGGCGATGAGCTTCCGTACGCGAATGCAGGATTCTTCGCGGCAGAGTACGGGGATTATTGGAGATTCATGAAGAAACTGACGATGACGGAGCTTTTATCGCAGCGGCAAGTTGAGAGATCGCGCGGTGTCCGGAGAGAAGAAATGTTGAAATTGTTGAGGAAATTGTGTGAGTGCGGCGAGAAGAAGGAGGCTGTGGATTTAGGGGCGGAGCTCGTCAAACTCACCAACAACAGCACTTGTAGGTTGGTGATGAGTACGAGATGTTCAGGTGATGATGATGAAGCGGAGAAGATAAGATTATTAGTAAAAGAAACATTTGAGATGGCTTCAAAAGTAGCATTTGGGGATGTGTTTGGATGGCCTTTAGAGCGGCTGGCGTTTTGGATGTTTGGAAGACAAGCAAGGGATGTGACTTTGAGGTATGATGAGATTTTGGAGAAAATATTAAGGCAACATGAAGATAGAGGAAAAAGAGAAGGTTTGGATAGGGAAGATAGAGATTTAATGGACATTTTGTTGAAGGTTTATCAAGATCACAATGCTGAGTTCAACATTACTAGAACCAATATCAAGGCTTTCTTGCTTGATTTATTTCTAGGCGGAACTGGTACATCAACAGAGGTATCACAGTGGACAATGGCGGAACTTCTGAACCATCCTAATGTATTCAACAAACTTAGAAACGAAATAAACTATGTCGTAGGAACCACCAGACTCGTCGGAGAAGACGACCTCCCAAATCTCCCTTACTTACAAGCTATCATGAAGGAGGCTCTCCGCCTCTACCCTGCTGTCCCAATTGCCATGAGAGTATGTCGTCAAGACTGCATAATCGACGGCTACGATATCCCCAAGGACACAATGGTTGCAGTCAACCTGTTCGACATAATGCGCGACCCAAAAATCTGGGAAAATCCAAACGAATTCGACCCCGAAAGATTCACTGGCGATGTGAAGTACGAGATTAAGGGGCAACAAAGTTTCAATTTTGTGCCCTTTGGTGGGGGAAGACGAGCTTGCCCTGGCTCCACTTTGGCCTTCAGTTTCATTAGCAATGTGATTGCAACAATGGTTCAGTGTTTTGATTGGAAAATTATTGGAAGACCAGATAATAATGAAGATGGAATAAGTAAAGTGGATATGGAGATTGGAGTTGCCTTTACTTTGCCCATGGCTAACCCTCTTCGTTGTGTTCCTATGGTTCGCTTCAACCCTTTTAATGACACTCTAAATGAATGA

Protein sequence

MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHNLAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAAEYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKLTNNSTCRLVMSTRCSGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAFLLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQAIMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPERFTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNEDGISKVDMEIGVAFTLPMANPLRCVPMVRFNPFNDTLNE
Homology
BLAST of Cucsat.G12141 vs. ExPASy Swiss-Prot
Match: Q9FI39 (Cytochrome P450 705A5 OS=Arabidopsis thaliana OX=3702 GN=CYP705A5 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.9e-112
Identity = 222/509 (43.61%), Postives = 322/509 (63.26%), Query Frame = 0

Query: 10  YIFLFF-FFTILLHYLFHRRSYAPRR----PPTPPALPLIGHLH--HFSPSVYKSFHNLA 69
           +IFL    F+ L + LF R++   R     PP+PP+LP+IGHLH   F P +++SF N++
Sbjct: 13  FIFLLLCLFSRLSYDLFFRKTKDSRAGCALPPSPPSLPIIGHLHLILFVP-IHQSFKNIS 72

Query: 70  AKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAAEY 129
           +KYG LL LR    P V++SSAS A EI+K QDVN SSRP     + L   ++ F    Y
Sbjct: 73  SKYGPLLHLRFFNFPIVLVSSASTAYEIFKAQDVNVSSRPPPPIEESLILGSSSFINTPY 132

Query: 130 GDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKLTN 189
           GDY +FMKK  + +LL  + ++RSR +R +E+ +  + L +   KK+ V++  E +KLTN
Sbjct: 133 GDYSKFMKKFMVQKLLGPQALQRSRNIRADELERFYKTLLDKAMKKQTVEIRNEAMKLTN 192

Query: 190 NSTCRLVMSTRCSGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQARD 249
           N+ C+++M   CS ++ EAE +R LV E+  +  K   G +F  PL++L   +F ++  +
Sbjct: 193 NTICKMIMGRSCSEENGEAETVRGLVTESIFLTKKHFLGAMFHKPLKKLGISLFAKELMN 252

Query: 250 VTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAFLLD 309
           V+ R+DE+LEKIL +HE++ +      +  D++D+LL+ Y D NAE+ ITR  IK+  +D
Sbjct: 253 VSNRFDELLEKILVEHEEKLQEH---HQTSDMLDMLLEAYGDENAEYKITRDQIKSLFVD 312

Query: 310 LFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQAIMK 369
           LF  GT  S    QWTMAE++ +P +  +LR EI+ VVG TRLV E DLPNLPYLQAI+K
Sbjct: 313 LFSAGTEASANTIQWTMAEIIKNPKICERLREEIDSVVGKTRLVQETDLPNLPYLQAIVK 372

Query: 370 EALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPERFTG 429
           E LRL+P  P+ +R  ++ C I G+ IP+ T + VN++ IMRDP  WE+P EF PERF  
Sbjct: 373 EGLRLHPPGPV-VRTFKETCEIKGFYIPEKTRLFVNVYAIMRDPDFWEDPEEFKPERFLA 432

Query: 430 DVKY---EIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 489
             +    + K +    ++PFG GRRACPGS LA++ + +VI  MVQ FDW I G      
Sbjct: 433 SSRLGEEDEKREDMLKYIPFGSGRRACPGSHLAYTVVGSVIGMMVQHFDWIIKG------ 492

Query: 490 DGISKVDMEIGVAFTLPMANPLRCVPMVR 509
               K++M+ G   TL MA+PL+C P+ R
Sbjct: 493 ---EKINMKEGGTMTLTMAHPLKCTPVPR 507

BLAST of Cucsat.G12141 vs. ExPASy Swiss-Prot
Match: Q9FH67 (Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 5.5e-111
Identity = 218/508 (42.91%), Postives = 311/508 (61.22%), Query Frame = 0

Query: 13  LFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHF--SPSVYKSFHNLAAKYGDLLF 72
           L   F+ L + LF ++      PP+PP+LP+IGHLHH   S   +KSF  L+ KYG LL 
Sbjct: 17  LLCLFSFLCYALFFKKPKGFDLPPSPPSLPIIGHLHHLLSSSLPHKSFQKLSFKYGPLLH 76

Query: 73  LRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAAEYGDYWRFMK 132
           LR+   P V++SSAS A E+++  DVN S R      D L + ++GF  A YGDYW+FMK
Sbjct: 77  LRIFNFPMVLVSSASMAYEVFRTNDVNVSYRFVPVNKDSLVFGSSGFVTAPYGDYWKFMK 136

Query: 133 KLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKLTNNSTCRLVM 192
           KL  T+LL    +E S+G R EE+ +    L     KKE+V++G   +KLTNN  CR+ M
Sbjct: 137 KLISTKLLRPHALELSKGNRAEELRRFCLDLQGKARKKESVEIGKVALKLTNNIICRMSM 196

Query: 193 STRCSGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQARDVTLRYDEI 252
              CS  +  AE+ R LV ++F ++ K+ F +            MF +    V+  +DE 
Sbjct: 197 GRSCSEKNGVAERARELVNKSFALSVKLFFSN------------MFRKDIMGVSREFDEF 256

Query: 253 LEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAFLLDLFLGGTGT 312
           LE+IL +HE+  + +    +DRD++D LL+ Y++  AE+ ITR  IK+ ++++FLGGT +
Sbjct: 257 LERILVEHEENLEGD----QDRDMIDHLLEAYRNEEAEYKITRKQIKSLIVEIFLGGTDS 316

Query: 313 STEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQAIMKEALRLYPA 372
           S +  QWTMAE+LN+P V  KLR EI+ VVG  RL+ E DLPNLPYLQA++KE LRL+P+
Sbjct: 317 SAQTIQWTMAEILNNPGVLEKLRAEIDSVVGGKRLIQESDLPNLPYLQAVVKEGLRLHPS 376

Query: 373 VPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPERF-----TGDVK 432
            P+ +RV  + C +  + +P+ T + VNL+ + RDP  WE+P+ F PERF     +GD  
Sbjct: 377 APVLLRVFGESCEVKEFYVPEKTTLVVNLYAVNRDPDSWEDPDMFKPERFLVSSISGD-- 436

Query: 433 YEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNEDGISKV 492
            E   +Q+  +V FGGGRR CP   LA  F+   I  MVQCFDW+I G     E+ +S  
Sbjct: 437 EEKIREQAVKYVTFGGGRRTCPAVKLAHIFMETAIGAMVQCFDWRIKGEKVYMEEAVS-- 496

Query: 493 DMEIGVAFTLPMANPLRCVPMVRFNPFN 514
                   +L MA+PL+C P+VRF+PF+
Sbjct: 497 ------GLSLKMAHPLKCTPVVRFDPFS 498

BLAST of Cucsat.G12141 vs. ExPASy Swiss-Prot
Match: Q9LJY5 (Cytochrome P450 705A22 OS=Arabidopsis thaliana OX=3702 GN=CYP705A22 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.2e-110
Identity = 210/512 (41.02%), Postives = 320/512 (62.50%), Query Frame = 0

Query: 10  YIF-LFFFFTILLHYLFHRRSYAPR----RPPTPPALPLIGHLH-HFSPSVYKSFHNLAA 69
           +IF L F  T L  + F ++    R     PP+PP+LP+IGH+H   S   +KS   L++
Sbjct: 13  FIFILIFLLTFLCFFFFFKKPKDSRVNFDLPPSPPSLPIIGHVHLLLSTLTHKSLQKLSS 72

Query: 70  KYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAAEYG 129
           +YG LL+LR+  VP +++SSAS A EI++ QDVN SSR   A  + L + ++ F  A YG
Sbjct: 73  RYGPLLYLRIFNVPIILVSSASVAYEIFRTQDVNISSRGVTAVDESLVFGSSSFVTAPYG 132

Query: 130 DYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKLTNN 189
           DYW+FMKKLT+ +LL  +  E+SR +R +++ +  R L +   KKE+V++G E + L NN
Sbjct: 133 DYWKFMKKLTVMKLLGPQAQEQSRDIRADDIKRFCRNLLDKARKKESVEIGKEAMNLMNN 192

Query: 190 STCRLVMSTRCSGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQARDV 249
             C++ M    S ++ E EK+R LV E+  +  K+    +    L++L   +F +    V
Sbjct: 193 ILCKMSMGRSFSEENGETEKLRGLVTESIGLMKKMFLAVLLRRQLQKLGISLFKKDIMGV 252

Query: 250 TLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAFLLDL 309
           + ++D +LEK+L +H ++ +++    +   ++D+LL  Y D NAE+ IT+ +IKAF +DL
Sbjct: 253 SNKFDVLLEKVLVEHREKPEKD----QGTVMLDVLLAAYGDENAEYKITKNHIKAFFVDL 312

Query: 310 FLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQAIMKE 369
           F+G T TS +  QWTMAE++N+ ++  ++R EI+ VVG +RL+ E DLPNLPYL A++KE
Sbjct: 313 FIGATDTSVQTIQWTMAEIMNNTHILERMREEIDSVVGKSRLIQETDLPNLPYLHAVIKE 372

Query: 370 ALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPERFTGD 429
           ALRL+P  P+  R  +Q C I G+ IP+ T + +N + +MRDP +WE+P EF PERF   
Sbjct: 373 ALRLHPPGPLLPREFQQGCKIGGFYIPEKTTLLINAYVVMRDPNVWEDPEEFKPERFLAS 432

Query: 430 VK---YEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNED 489
            +    + + +Q+  F+PFG GRR CPGS LA+  + + I  MVQCFDW+I G       
Sbjct: 433 SRSGQEDERREQALKFLPFGSGRRGCPGSNLAYMIVGSAIGMMVQCFDWRIEG------- 492

Query: 490 GISKVDMEIGVAFT-LPMANPLRCVPMVRFNP 512
              KV+M+  V  T L MA+PL+  P+ R  P
Sbjct: 493 --EKVNMKEAVKGTILTMAHPLKLTPVTRQPP 511

BLAST of Cucsat.G12141 vs. ExPASy Swiss-Prot
Match: Q42798 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.3e-109
Identity = 209/503 (41.55%), Postives = 317/503 (63.02%), Query Frame = 0

Query: 18  TILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHNLAAKYGDLLFLRLGRVP 77
           TI+  Y+  R+      PP+P ALP+IGHLH  SP  ++ F+ L+ ++G ++ L LG VP
Sbjct: 13  TIVFAYILWRKQSKKNLPPSPKALPIIGHLHLVSPIPHQDFYKLSTRHGPIMQLFLGSVP 72

Query: 78  CVVISSASYAAEIYKNQDVNFSSRP-KFAFGDELPYANAGF-FA-AEYGDYWRFMKKLTM 137
           CVV S+A  A E  K  ++NFS+RP +      L Y +  F FA A +G YW+FMKKL M
Sbjct: 73  CVVASTAEAAKEFLKTHEINFSNRPGQNVAVKGLAYDSQDFLFAFAPFGPYWKFMKKLCM 132

Query: 138 TELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKLTNNSTCRLVMSTRC 197
           +ELLS R +++   VR++E  + + ++   G   EAVD G EL+ L+NN   R+ +S + 
Sbjct: 133 SELLSGRMMDQFLPVRQQETKRFISRVFRKGVAGEAVDFGDELMTLSNNIVSRMTLSQKT 192

Query: 198 SGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQARDVTLRYDEILEKI 257
           S +D++AE+++ LV    E+  K    D F W L+      F R+ ++   R+D +++ I
Sbjct: 193 SENDNQAEEMKKLVSNIAELMGKFNVSD-FIWYLKPFDLQGFNRKIKETRDRFDVVVDGI 252

Query: 258 LRQHED---RGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAFLLDLFLGGTGTS 317
           ++Q ++   + K  G  ++ +D++D+LL +++D NAE  + + NIKAF++D+F+ GT TS
Sbjct: 253 IKQRQEERRKNKETGTAKQFKDMLDVLLDMHEDENAEIKLDKKNIKAFIMDIFVAGTDTS 312

Query: 318 TEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQAIMKEALRLYPAV 377
               +W MAEL+N+P+V  K R EI+ VVG +R+V E D+ NLPYLQAI++E LRL+P  
Sbjct: 313 AVSIEWAMAELINNPDVLEKARQEIDAVVGKSRMVEESDIANLPYLQAIVRETLRLHPGG 372

Query: 378 PIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPERF--TGDVKYEIK 437
           P+ +R   +  ++ GYDIP  T + VN++ I RDP  WE P EF PERF   G  + +++
Sbjct: 373 PLVVRESSKSAVVCGYDIPAKTRLFVNVWAIGRDPNHWEKPFEFRPERFIRDGQNQLDVR 432

Query: 438 GQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNEDGISKVDMEI 497
           GQ  ++F+PFG GRR CPG++LA+  +   +A ++QCF WK++G       G  KVDME 
Sbjct: 433 GQH-YHFIPFGSGRRTCPGASLAWQVVPVNLAIIIQCFQWKLVG-------GNGKVDMEE 492

Query: 498 GVAFTLPMANPLRCVPMVRFNPF 513
               TLP ANP+ CVP+ R NPF
Sbjct: 493 KSGITLPRANPIICVPVPRINPF 506

BLAST of Cucsat.G12141 vs. ExPASy Swiss-Prot
Match: Q9LJY7 (Cytochrome P450 705A20 OS=Arabidopsis thaliana OX=3702 GN=CYP705A20 PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.1e-107
Identity = 217/514 (42.22%), Postives = 310/514 (60.31%), Query Frame = 0

Query: 3   DSTSYLLYIFLFFFFTILLHYLFH--RRSYAPR-RPPTPPALPLIGHLHHFSPSV-YKSF 62
           DS     +I L FF  +    LF   + S+  R    +PP+LP+IGHLHH   S+ +KS 
Sbjct: 5   DSQHCFSFILLCFFSLLCYSLLFKKLKDSHVGRDLLQSPPSLPIIGHLHHLLSSLAHKSL 64

Query: 63  HNLAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFF 122
             L++KYG LL L +   P V++SSAS A EI+K  D+N SSR      + L   ++ F 
Sbjct: 65  QQLSSKYGPLLHLSIFNFPVVLVSSASVAYEIFKAHDLNISSRDNPPINESLLVGSSVFV 124

Query: 123 AAEYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELV 182
            A YGDYW+FMKKL +T+LL  + +ERSR +R +E+ +  R L +   KKE+V++G E  
Sbjct: 125 GAPYGDYWKFMKKLLVTKLLGPQALERSRSIRADELERFYRSLLDKAMKKESVEIGKEAT 184

Query: 183 KLTNNSTCRLVMSTRCSGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGR 242
           KL+ NS CR+ M    S +  EAE++R LV E   +  KV   ++  WPLE+L   +F +
Sbjct: 185 KLSINSICRMSMGRSFSEESGEAERVRGLVTELDGLTKKVLLVNILRWPLEKLRISLFKK 244

Query: 243 QARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKA 302
           +   V+  +DE+LE+I+ + E +      + +   LMD+LL+ Y+D  AE  ITR +IK+
Sbjct: 245 EIMYVSNSFDELLERIIVEREKKPN----EHQGTYLMDVLLEAYEDEKAEHKITRNHIKS 304

Query: 303 FLLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQ 362
             ++L LGGT TS +  QWTMAEL+N+ NV  +LR EI+ VVG TRL+ E DLP LPYLQ
Sbjct: 305 LFVELLLGGTDTSAQTIQWTMAELINNRNVLKRLREEIDSVVGETRLIQEKDLPKLPYLQ 364

Query: 363 AIMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPE 422
           +++KE LRL+P +P+ +R  ++ C + G+ I + T + VN + +MRDP  WE+P+EF PE
Sbjct: 365 SVVKEGLRLHPPLPLMVRTFQRSCEMKGFYIAEKTTLVVNAYAVMRDPTTWEDPDEFKPE 424

Query: 423 RFTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNN 482
           RF      + + +++   + FG GRR CPGS LA  FI   I TMVQCFD  I G     
Sbjct: 425 RFL----RQEEERRALKHIAFGSGRRGCPGSNLATIFIGTAIGTMVQCFDLSIKG----- 484

Query: 483 EDGISKVDMEIGVAFTLPMANPLRCVPMVRFNPF 513
                KV M+      L MA+PL C+ + R  PF
Sbjct: 485 ----DKVKMDEVGGLNLTMAHPLECILVPRTQPF 501

BLAST of Cucsat.G12141 vs. NCBI nr
Match: XP_011651789.1 (cytochrome P450 705A12 [Cucumis sativus] >KAE8650937.1 hypothetical protein Csa_001311 [Cucumis sativus])

HSP 1 Score: 1046 bits (2705), Expect = 0.0
Identity = 516/518 (99.61%), Postives = 517/518 (99.81%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN
Sbjct: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LA KYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA
Sbjct: 61  LATKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180

Query: 181 TNNSTCRLVMSTRCSGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQA 240
           TNNSTCRLVMSTRCSGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQA
Sbjct: 181 TNNSTCRLVMSTRCSGDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQA 240

Query: 241 RDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAFL 300
           RDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAFL
Sbjct: 241 RDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAFL 300

Query: 301 LDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQAI 360
           LDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQAI
Sbjct: 301 LDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQAI 360

Query: 361 MKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPERF 420
           MKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPERF
Sbjct: 361 MKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPERF 420

Query: 421 TGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNED 480
           TGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIG+PDNNED
Sbjct: 421 TGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGKPDNNED 480

Query: 481 GISKVDMEIGVAFTLPMANPLRCVPMVRFNPFNDTLNE 518
           GISKVDMEIGVAFTLPMANPLRCVPMVRFNPFNDTLNE
Sbjct: 481 GISKVDMEIGVAFTLPMANPLRCVPMVRFNPFNDTLNE 518

BLAST of Cucsat.G12141 vs. NCBI nr
Match: KAA0048325.1 (cytochrome P450 705A5-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 481/514 (93.58%), Postives = 493/514 (95.91%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADSTSY+LYI LFFFFTILLHYL H RSYA  RPP+PPALPLIGHLHHFSPSVYKSFHN
Sbjct: 1   MADSTSYVLYIILFFFFTILLHYLLHPRSYARPRPPSPPALPLIGHLHHFSPSVYKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LAAKYGDLLFLRLGRV CVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYA AGFFAA
Sbjct: 61  LAAKYGDLLFLRLGRVRCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYAKAGFFAA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKE VDLGAELVKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEVVDLGAELVKL 180

Query: 181 TNNSTCRLVMSTRCSGDDD-EAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240
           TNNSTCRLVMSTRCSGDDD EAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ
Sbjct: 181 TNNSTCRLVMSTRCSGDDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240

Query: 241 ARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300
           A+DVTLRYDEILE ILRQHEDRG+ EGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF
Sbjct: 241 AKDVTLRYDEILENILRQHEDRGREEGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300

Query: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQA 360
           LLDLFLGGTGTSTEVSQWTMAELLNHP VFNKLRNEIN +VGT+RLVGEDDLPNLPYLQA
Sbjct: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPKVFNKLRNEINSIVGTSRLVGEDDLPNLPYLQA 360

Query: 361 IMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPER 420
           ++KEALRLYPAVPIAMR CRQDC IDGY IPKDTMVAVNLFDIMRDPKIW+N NEFDPER
Sbjct: 361 VVKEALRLYPAVPIAMRTCRQDCTIDGYHIPKDTMVAVNLFDIMRDPKIWKNSNEFDPER 420

Query: 421 FTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 480
           FTGDV+YE KGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGR DNN 
Sbjct: 421 FTGDVQYEFKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRSDNNR 480

Query: 481 DGISKVDMEIGVAFTLPMANPLRCVPMVRFNPFN 513
           DGISKVDMEIG AFTLPMANPLRCVP+VRFNPF+
Sbjct: 481 DGISKVDMEIGAAFTLPMANPLRCVPIVRFNPFD 514

BLAST of Cucsat.G12141 vs. NCBI nr
Match: KAA0048326.1 (cytochrome P450 705A5-like protein [Cucumis melo var. makuwa] >TYK10669.1 cytochrome P450 705A5-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 465/518 (89.77%), Postives = 488/518 (94.21%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADST Y LYI LFFFFTILL YLFH RSYA RRPP+PPALPLIGHLHHFSP V+KSFHN
Sbjct: 1   MADSTYYALYIILFFFFTILLRYLFHPRSYARRRPPSPPALPLIGHLHHFSPYVHKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LAAKYGDLLFLRLGRV CVVISSA+YAAEIYKNQDVNFSSRPK AFGDE+PYA AGFF A
Sbjct: 61  LAAKYGDLLFLRLGRVRCVVISSATYAAEIYKNQDVNFSSRPKLAFGDEIPYAKAGFFVA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKE VDLG E+VKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEVVDLGTEIVKL 180

Query: 181 TNNSTCRLVMSTRCSGD-DDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240
           TNNSTCRLVMSTRCSG+ DDEAEKIRLL+KETFE+ASK++FGDVFGWPLERLAFWMFGRQ
Sbjct: 181 TNNSTCRLVMSTRCSGNGDDEAEKIRLLIKETFEIASKISFGDVFGWPLERLAFWMFGRQ 240

Query: 241 ARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300
           A+DVTLRYDEILE ILRQHEDRG+ EGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF
Sbjct: 241 AKDVTLRYDEILENILRQHEDRGREEGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300

Query: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQA 360
           LLDLFLGGT TSTEVSQW MAELLNHP VFNKLRNEIN VVGTTRLV EDDLPNLPYLQA
Sbjct: 301 LLDLFLGGTATSTEVSQWAMAELLNHPKVFNKLRNEINSVVGTTRLVDEDDLPNLPYLQA 360

Query: 361 IMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPER 420
           ++KEALRLYPAVPI+MR CRQDC ID YDIPKDT+VAVNLF IMRDPKIWENPN+F+PER
Sbjct: 361 VVKEALRLYPAVPISMRACRQDCTIDNYDIPKDTLVAVNLFTIMRDPKIWENPNKFNPER 420

Query: 421 FTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 480
           FTGDV+YEIKGQQ+FNFVPFGGGRRACPGSTLAFSFI N+IATMVQCFDWKIIG+ DNN 
Sbjct: 421 FTGDVEYEIKGQQNFNFVPFGGGRRACPGSTLAFSFIRNLIATMVQCFDWKIIGKSDNNG 480

Query: 481 DGISKVDMEIGVAFTLPMANPLRCVPMVRFNPFNDTLN 517
           DGISKVDMEIG AFTLPMANPLRCVP+V FNPFNDTLN
Sbjct: 481 DGISKVDMEIGAAFTLPMANPLRCVPIVHFNPFNDTLN 518

BLAST of Cucsat.G12141 vs. NCBI nr
Match: XP_038905910.1 (cytochrome P450 705A5-like [Benincasa hispida])

HSP 1 Score: 924 bits (2389), Expect = 0.0
Identity = 462/512 (90.23%), Postives = 483/512 (94.34%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADSTSY+LYI LFFFFTILL YL  R S+  RRPP+PPALPLIGHLHHFSPSV KSFHN
Sbjct: 1   MADSTSYILYIILFFFFTILLQYLLQRPSHG-RRPPSPPALPLIGHLHHFSPSVCKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LAA+YG+LLFLRLGRV CVV+SSASYAAEIYKNQD+NFSSRPKFAFGDELPYA AGFFAA
Sbjct: 61  LAARYGNLLFLRLGRVRCVVVSSASYAAEIYKNQDINFSSRPKFAFGDELPYAKAGFFAA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKL ECGEKKEAVDLGAELVKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLWECGEKKEAVDLGAELVKL 180

Query: 181 TNNSTCRLVMSTRCSGDDD-EAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240
           TNNSTCRLVMSTRCSGDDD EAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGR 
Sbjct: 181 TNNSTCRLVMSTRCSGDDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRH 240

Query: 241 ARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300
           A+DVT+RYDEILEKILRQHE+R + EGLDRED DLMDILLKVYQDHNAEFNITRTNIKAF
Sbjct: 241 AKDVTMRYDEILEKILRQHEERAREEGLDREDTDLMDILLKVYQDHNAEFNITRTNIKAF 300

Query: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQA 360
           LLDLFLGGTGTSTEVSQW MAELLNHP V NKLRNEIN VVGTTR+VGEDDLPNLPYLQA
Sbjct: 301 LLDLFLGGTGTSTEVSQWAMAELLNHPKVLNKLRNEINSVVGTTRVVGEDDLPNLPYLQA 360

Query: 361 IMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPER 420
           ++KEALRLYPAVPIAMR CRQDC IDG+DIPKDTMVAVNLFDIMRDPKIW+NPNEFDPER
Sbjct: 361 VVKEALRLYPAVPIAMRACRQDCKIDGFDIPKDTMVAVNLFDIMRDPKIWQNPNEFDPER 420

Query: 421 FTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 480
           FTGDV+YEIKGQQSF+FVPFGGGRRACPGSTLAFSFISNVIA MVQCFDWK+IGR DN  
Sbjct: 421 FTGDVRYEIKGQQSFDFVPFGGGRRACPGSTLAFSFISNVIAAMVQCFDWKVIGRDDNG- 480

Query: 481 DGISKVDMEIGVAFTLPMANPLRCVPMVRFNP 511
           DG SKV+MEIG AFTLPMANPL CVP+VRFNP
Sbjct: 481 DGRSKVNMEIGAAFTLPMANPLLCVPVVRFNP 510

BLAST of Cucsat.G12141 vs. NCBI nr
Match: XP_022983262.1 (cytochrome P450 705A5-like [Cucurbita maxima])

HSP 1 Score: 868 bits (2242), Expect = 4.54e-315
Identity = 437/510 (85.69%), Postives = 459/510 (90.00%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADSTSYLLYI LFF F ILL YL HR S   R PP+PPALPLIGHLHHFSPSV KSFHN
Sbjct: 1   MADSTSYLLYIALFFLFIILLQYLLHRPSNG-RTPPSPPALPLIGHLHHFSPSVCKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LAA+YGDLLFLRLG V CVV+SSASYAAEIYK QDV FSSRPKFAFGDELPYA AGFFAA
Sbjct: 61  LAARYGDLLFLRLGSVRCVVVSSASYAAEIYKTQDVTFSSRPKFAFGDELPYAKAGFFAA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSR VRREEMLK L+KL +CGEK EAVDLGAELVKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRAVRREEMLKFLKKLADCGEKNEAVDLGAELVKL 180

Query: 181 TNNSTCRLVMSTRCSGDD-DEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240
           TNNSTCRLVMSTRCSGDD DEAEKIR+LVKETFEMA+KVAFGDVFGWPLERLAFWMFG+ 
Sbjct: 181 TNNSTCRLVMSTRCSGDDSDEAEKIRVLVKETFEMATKVAFGDVFGWPLERLAFWMFGKH 240

Query: 241 ARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300
           A+DVT+RYDEILEKILRQHE+R K EGLDREDRDLMDILLKVYQDHNAEF ITRTNIKAF
Sbjct: 241 AKDVTMRYDEILEKILRQHEERAKEEGLDREDRDLMDILLKVYQDHNAEFKITRTNIKAF 300

Query: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQA 360
            LDLFLGGTGTSTEVSQWTMAELLNHP VFNKLRNEIN VVGTTRLVGEDD+PNLPYLQA
Sbjct: 301 FLDLFLGGTGTSTEVSQWTMAELLNHPEVFNKLRNEINAVVGTTRLVGEDDVPNLPYLQA 360

Query: 361 IMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPER 420
           ++KEALRLYPAVPIAMR  R+DC IDG+ +PKDTMVAVNLF IMRDPKIWENPNEF+PER
Sbjct: 361 VVKEALRLYPAVPIAMRASREDCTIDGFHVPKDTMVAVNLFHIMRDPKIWENPNEFEPER 420

Query: 421 FTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 480
           FTG+V+YEIKGQQ   FVPFGGGRR CPGSTLAFSFIS V A MVQCFDWKIIG      
Sbjct: 421 FTGEVRYEIKGQQGSGFVPFGGGRRGCPGSTLAFSFISTVTAAMVQCFDWKIIGG----- 480

Query: 481 DGISKVDMEIGVAFTLPMANPLRCVPMVRF 509
              SKVDMEIG AFTLPMANPL CVP++RF
Sbjct: 481 ---SKVDMEIGAAFTLPMANPLLCVPLLRF 501

BLAST of Cucsat.G12141 vs. ExPASy TrEMBL
Match: A0A5A7U3V6 (Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00360 PE=3 SV=1)

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 481/514 (93.58%), Postives = 493/514 (95.91%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADSTSY+LYI LFFFFTILLHYL H RSYA  RPP+PPALPLIGHLHHFSPSVYKSFHN
Sbjct: 1   MADSTSYVLYIILFFFFTILLHYLLHPRSYARPRPPSPPALPLIGHLHHFSPSVYKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LAAKYGDLLFLRLGRV CVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYA AGFFAA
Sbjct: 61  LAAKYGDLLFLRLGRVRCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYAKAGFFAA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKE VDLGAELVKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEVVDLGAELVKL 180

Query: 181 TNNSTCRLVMSTRCSGDDD-EAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240
           TNNSTCRLVMSTRCSGDDD EAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ
Sbjct: 181 TNNSTCRLVMSTRCSGDDDDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240

Query: 241 ARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300
           A+DVTLRYDEILE ILRQHEDRG+ EGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF
Sbjct: 241 AKDVTLRYDEILENILRQHEDRGREEGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300

Query: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQA 360
           LLDLFLGGTGTSTEVSQWTMAELLNHP VFNKLRNEIN +VGT+RLVGEDDLPNLPYLQA
Sbjct: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPKVFNKLRNEINSIVGTSRLVGEDDLPNLPYLQA 360

Query: 361 IMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPER 420
           ++KEALRLYPAVPIAMR CRQDC IDGY IPKDTMVAVNLFDIMRDPKIW+N NEFDPER
Sbjct: 361 VVKEALRLYPAVPIAMRTCRQDCTIDGYHIPKDTMVAVNLFDIMRDPKIWKNSNEFDPER 420

Query: 421 FTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 480
           FTGDV+YE KGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGR DNN 
Sbjct: 421 FTGDVQYEFKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRSDNNR 480

Query: 481 DGISKVDMEIGVAFTLPMANPLRCVPMVRFNPFN 513
           DGISKVDMEIG AFTLPMANPLRCVP+VRFNPF+
Sbjct: 481 DGISKVDMEIGAAFTLPMANPLRCVPIVRFNPFD 514

BLAST of Cucsat.G12141 vs. ExPASy TrEMBL
Match: A0A5A7U271 (Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00310 PE=3 SV=1)

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 465/518 (89.77%), Postives = 488/518 (94.21%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADST Y LYI LFFFFTILL YLFH RSYA RRPP+PPALPLIGHLHHFSP V+KSFHN
Sbjct: 1   MADSTYYALYIILFFFFTILLRYLFHPRSYARRRPPSPPALPLIGHLHHFSPYVHKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LAAKYGDLLFLRLGRV CVVISSA+YAAEIYKNQDVNFSSRPK AFGDE+PYA AGFF A
Sbjct: 61  LAAKYGDLLFLRLGRVRCVVISSATYAAEIYKNQDVNFSSRPKLAFGDEIPYAKAGFFVA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKE VDLG E+VKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEVVDLGTEIVKL 180

Query: 181 TNNSTCRLVMSTRCSGD-DDEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240
           TNNSTCRLVMSTRCSG+ DDEAEKIRLL+KETFE+ASK++FGDVFGWPLERLAFWMFGRQ
Sbjct: 181 TNNSTCRLVMSTRCSGNGDDEAEKIRLLIKETFEIASKISFGDVFGWPLERLAFWMFGRQ 240

Query: 241 ARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300
           A+DVTLRYDEILE ILRQHEDRG+ EGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF
Sbjct: 241 AKDVTLRYDEILENILRQHEDRGREEGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300

Query: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQA 360
           LLDLFLGGT TSTEVSQW MAELLNHP VFNKLRNEIN VVGTTRLV EDDLPNLPYLQA
Sbjct: 301 LLDLFLGGTATSTEVSQWAMAELLNHPKVFNKLRNEINSVVGTTRLVDEDDLPNLPYLQA 360

Query: 361 IMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPER 420
           ++KEALRLYPAVPI+MR CRQDC ID YDIPKDT+VAVNLF IMRDPKIWENPN+F+PER
Sbjct: 361 VVKEALRLYPAVPISMRACRQDCTIDNYDIPKDTLVAVNLFTIMRDPKIWENPNKFNPER 420

Query: 421 FTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 480
           FTGDV+YEIKGQQ+FNFVPFGGGRRACPGSTLAFSFI N+IATMVQCFDWKIIG+ DNN 
Sbjct: 421 FTGDVEYEIKGQQNFNFVPFGGGRRACPGSTLAFSFIRNLIATMVQCFDWKIIGKSDNNG 480

Query: 481 DGISKVDMEIGVAFTLPMANPLRCVPMVRFNPFNDTLN 517
           DGISKVDMEIG AFTLPMANPLRCVP+V FNPFNDTLN
Sbjct: 481 DGISKVDMEIGAAFTLPMANPLRCVPIVHFNPFNDTLN 518

BLAST of Cucsat.G12141 vs. ExPASy TrEMBL
Match: A0A6J1IYT7 (cytochrome P450 705A5-like OS=Cucurbita maxima OX=3661 GN=LOC111481896 PE=3 SV=1)

HSP 1 Score: 868 bits (2242), Expect = 2.20e-315
Identity = 437/510 (85.69%), Postives = 459/510 (90.00%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADSTSYLLYI LFF F ILL YL HR S   R PP+PPALPLIGHLHHFSPSV KSFHN
Sbjct: 1   MADSTSYLLYIALFFLFIILLQYLLHRPSNG-RTPPSPPALPLIGHLHHFSPSVCKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LAA+YGDLLFLRLG V CVV+SSASYAAEIYK QDV FSSRPKFAFGDELPYA AGFFAA
Sbjct: 61  LAARYGDLLFLRLGSVRCVVVSSASYAAEIYKTQDVTFSSRPKFAFGDELPYAKAGFFAA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSR VRREEMLK L+KL +CGEK EAVDLGAELVKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRAVRREEMLKFLKKLADCGEKNEAVDLGAELVKL 180

Query: 181 TNNSTCRLVMSTRCSGDD-DEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240
           TNNSTCRLVMSTRCSGDD DEAEKIR+LVKETFEMA+KVAFGDVFGWPLERLAFWMFG+ 
Sbjct: 181 TNNSTCRLVMSTRCSGDDSDEAEKIRVLVKETFEMATKVAFGDVFGWPLERLAFWMFGKH 240

Query: 241 ARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300
           A+DVT+RYDEILEKILRQHE+R K EGLDREDRDLMDILLKVYQDHNAEF ITRTNIKAF
Sbjct: 241 AKDVTMRYDEILEKILRQHEERAKEEGLDREDRDLMDILLKVYQDHNAEFKITRTNIKAF 300

Query: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQA 360
            LDLFLGGTGTSTEVSQWTMAELLNHP VFNKLRNEIN VVGTTRLVGEDD+PNLPYLQA
Sbjct: 301 FLDLFLGGTGTSTEVSQWTMAELLNHPEVFNKLRNEINAVVGTTRLVGEDDVPNLPYLQA 360

Query: 361 IMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPER 420
           ++KEALRLYPAVPIAMR  R+DC IDG+ +PKDTMVAVNLF IMRDPKIWENPNEF+PER
Sbjct: 361 VVKEALRLYPAVPIAMRASREDCTIDGFHVPKDTMVAVNLFHIMRDPKIWENPNEFEPER 420

Query: 421 FTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 480
           FTG+V+YEIKGQQ   FVPFGGGRR CPGSTLAFSFIS V A MVQCFDWKIIG      
Sbjct: 421 FTGEVRYEIKGQQGSGFVPFGGGRRGCPGSTLAFSFISTVTAAMVQCFDWKIIGG----- 480

Query: 481 DGISKVDMEIGVAFTLPMANPLRCVPMVRF 509
              SKVDMEIG AFTLPMANPL CVP++RF
Sbjct: 481 ---SKVDMEIGAAFTLPMANPLLCVPLLRF 501

BLAST of Cucsat.G12141 vs. ExPASy TrEMBL
Match: A0A6J1F426 (cytochrome P450 705A22-like OS=Cucurbita moschata OX=3662 GN=LOC111441932 PE=3 SV=1)

HSP 1 Score: 858 bits (2216), Expect = 1.82e-311
Identity = 429/511 (83.95%), Postives = 458/511 (89.63%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFTILLHYLFHRRSYAPRRPPTPPALPLIGHLHHFSPSVYKSFHN 60
           MADSTSY+ YI L F F ILLHYL HR S   R PP+PPALPLIGHLHHFSPSV KSFHN
Sbjct: 1   MADSTSYIFYIALSFLFIILLHYLLHRPSNG-RTPPSPPALPLIGHLHHFSPSVCKSFHN 60

Query: 61  LAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAGFFAA 120
           LAA+YGDLLFLRLG V CVV+SSASYA EIYK QDV FSSRPKFAFGDELPYA AGFFAA
Sbjct: 61  LAARYGDLLFLRLGSVRCVVVSSASYATEIYKTQDVTFSSRPKFAFGDELPYAKAGFFAA 120

Query: 121 EYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAELVKL 180
           EYGDYWRFMKKLTMTELLSQRQVERSR VRREEMLK L+KL +CGE  EAVDLGAELVKL
Sbjct: 121 EYGDYWRFMKKLTMTELLSQRQVERSRAVRREEMLKFLKKLADCGENNEAVDLGAELVKL 180

Query: 181 TNNSTCRLVMSTRCSGDD-DEAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWMFGRQ 240
           TNNSTCRLVMSTRCSGDD DEAEKIR+LVKETFEMA+KVAFGDVFGWPLER+AFWMFG+ 
Sbjct: 181 TNNSTCRLVMSTRCSGDDSDEAEKIRILVKETFEMATKVAFGDVFGWPLERIAFWMFGKH 240

Query: 241 ARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTNIKAF 300
           A+DVT+RYD+ILE ILRQHE+R K+EGLDREDRDLMDILLKVYQDHNAEF ITRTNIKAF
Sbjct: 241 AKDVTMRYDQILENILRQHEERAKKEGLDREDRDLMDILLKVYQDHNAEFKITRTNIKAF 300

Query: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLPYLQA 360
           LLDLFLGGTGTSTEVSQWTMAELLNHP VFNKLRNEIN VVGTTRLVGEDD+PNLPYLQA
Sbjct: 301 LLDLFLGGTGTSTEVSQWTMAELLNHPEVFNKLRNEINAVVGTTRLVGEDDVPNLPYLQA 360

Query: 361 IMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEFDPER 420
           ++KEALRLYPAVPIAMR  ++DC IDG+ +PK+TMVAVNLF IMRDPKIWENPNEF+PER
Sbjct: 361 VVKEALRLYPAVPIAMRASQEDCTIDGFHVPKNTMVAVNLFHIMRDPKIWENPNEFEPER 420

Query: 421 FTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRPDNNE 480
           FTG+V+YEIKGQQ   FVPFGGGRR CPGSTLAFSFIS V A MVQCFDWKIIG      
Sbjct: 421 FTGEVRYEIKGQQGSGFVPFGGGRRGCPGSTLAFSFISTVTAAMVQCFDWKIIGG----- 480

Query: 481 DGISKVDMEIGVAFTLPMANPLRCVPMVRFN 510
              SKVDMEIG AFTLPMANPL CVP++RF+
Sbjct: 481 ---SKVDMEIGAAFTLPMANPLLCVPLLRFH 502

BLAST of Cucsat.G12141 vs. ExPASy TrEMBL
Match: A0A6J1DFG8 (cytochrome P450 705A5-like OS=Momordica charantia OX=3673 GN=LOC111020330 PE=3 SV=1)

HSP 1 Score: 833 bits (2152), Expect = 1.36e-301
Identity = 423/517 (81.82%), Postives = 455/517 (88.01%), Query Frame = 0

Query: 1   MADSTSYLLYIFLFFFFT-ILLHYLFHRRSYAPR---RPPTPPALPLIGHLHHFSPSVYK 60
           MADS SY+LY   FF FT ILL +LF + S       RPP+PPALPLIGHLH+FSPS  K
Sbjct: 1   MADSISYVLYTIFFFLFTVILLQFLFRKSSKNLAQGCRPPSPPALPLIGHLHYFSPSAIK 60

Query: 61  SFHNLAAKYGDLLFLRLGRVPCVVISSASYAAEIYKNQDVNFSSRPKFAFGDELPYANAG 120
           SFHNLAA+YGDLLFLRLG V CVV+SSAS+AAEIYKNQDV FS RP FAFGDEL YA AG
Sbjct: 61  SFHNLAARYGDLLFLRLGAVRCVVVSSASHAAEIYKNQDVAFSWRPNFAFGDELLYAKAG 120

Query: 121 FFAAEYGDYWRFMKKLTMTELLSQRQVERSRGVRREEMLKLLRKLCECGEKKEAVDLGAE 180
           FFAAEYGDYWRFMKKLTMTELLSQRQVERSR VRREEM+K LRKL E G K EAVDLGAE
Sbjct: 121 FFAAEYGDYWRFMKKLTMTELLSQRQVERSRAVRREEMVKFLRKLAESGHKNEAVDLGAE 180

Query: 181 LVKLTNNSTCRLVMSTRCSGDDD-EAEKIRLLVKETFEMASKVAFGDVFGWPLERLAFWM 240
           LVKLTNNSTCR+VMSTRCSGDDD EAEKIR+LVKETF+MA++VAFGDVFGWPLERLAFW+
Sbjct: 181 LVKLTNNSTCRMVMSTRCSGDDDDEAEKIRVLVKETFQMATQVAFGDVFGWPLERLAFWL 240

Query: 241 FGRQARDVTLRYDEILEKILRQHEDRGKREGLDREDRDLMDILLKVYQDHNAEFNITRTN 300
           FGRQA+DVTLRYDEILEKIL+QHE+R K EGLD EDRDLMDILLKVYQDHNAEF ITRTN
Sbjct: 241 FGRQAQDVTLRYDEILEKILKQHEERAKEEGLDGEDRDLMDILLKVYQDHNAEFKITRTN 300

Query: 301 IKAFLLDLFLGGTGTSTEVSQWTMAELLNHPNVFNKLRNEINYVVGTTRLVGEDDLPNLP 360
           IKAFLLDLFLGGTGTSTEVSQW MAELLNHP VF KLR+EI+ ++GT RLVGEDDLPNLP
Sbjct: 301 IKAFLLDLFLGGTGTSTEVSQWAMAELLNHPKVFTKLRDEIDSIIGTDRLVGEDDLPNLP 360

Query: 361 YLQAIMKEALRLYPAVPIAMRVCRQDCIIDGYDIPKDTMVAVNLFDIMRDPKIWENPNEF 420
           YLQA++KEALRLYPAVP++MR CR+DC IDGY +PK+TMVAVNLFDIMRDPK WENPNEF
Sbjct: 361 YLQAVVKEALRLYPAVPLSMRSCREDCTIDGYVVPKNTMVAVNLFDIMRDPKAWENPNEF 420

Query: 421 DPERFTGDVKYEIKGQQSFNFVPFGGGRRACPGSTLAFSFISNVIATMVQCFDWKIIGRP 480
           DPERF GD+KYEIKGQQSF FVPFGGGRRACPGS LAFSFISNVIA MVQCFDWKI    
Sbjct: 421 DPERFNGDMKYEIKGQQSFKFVPFGGGRRACPGSNLAFSFISNVIAAMVQCFDWKIGRNG 480

Query: 481 DNNEDGISKVDMEIGVAFTLPMANPLRCVPMVRFNPF 512
           D N     KV+MEIG AFTLPMANPL CVP+VRFN F
Sbjct: 481 DEN-----KVNMEIGSAFTLPMANPLLCVPVVRFNFF 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FI392.9e-11243.61Cytochrome P450 705A5 OS=Arabidopsis thaliana OX=3702 GN=CYP705A5 PE=2 SV=1[more]
Q9FH675.5e-11142.91Cytochrome P450 705A12 OS=Arabidopsis thaliana OX=3702 GN=CYP705A12 PE=3 SV=1[more]
Q9LJY51.2e-11041.02Cytochrome P450 705A22 OS=Arabidopsis thaliana OX=3702 GN=CYP705A22 PE=2 SV=1[more]
Q427981.3e-10941.553,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1... [more]
Q9LJY72.1e-10742.22Cytochrome P450 705A20 OS=Arabidopsis thaliana OX=3702 GN=CYP705A20 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_011651789.10.099.61cytochrome P450 705A12 [Cucumis sativus] >KAE8650937.1 hypothetical protein Csa_... [more]
KAA0048325.10.093.58cytochrome P450 705A5-like protein [Cucumis melo var. makuwa][more]
KAA0048326.10.089.77cytochrome P450 705A5-like protein [Cucumis melo var. makuwa] >TYK10669.1 cytoch... [more]
XP_038905910.10.090.23cytochrome P450 705A5-like [Benincasa hispida][more]
XP_022983262.14.54e-31585.69cytochrome P450 705A5-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A5A7U3V60.093.58Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5A7U2710.089.77Cytochrome P450 705A5-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1IYT72.20e-31585.69cytochrome P450 705A5-like OS=Cucurbita maxima OX=3661 GN=LOC111481896 PE=3 SV=1[more]
A0A6J1F4261.82e-31183.95cytochrome P450 705A22-like OS=Cucurbita moschata OX=3662 GN=LOC111441932 PE=3 S... [more]
A0A6J1DFG81.36e-30181.82cytochrome P450 705A5-like OS=Momordica charantia OX=3673 GN=LOC111020330 PE=3 S... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 306..323
score: 25.58
coord: 437..446
score: 57.4
coord: 446..457
score: 31.79
coord: 359..370
score: 42.12
IPR001128Cytochrome P450PFAMPF00067p450coord: 35..473
e-value: 8.3E-93
score: 311.6
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 295..312
score: 35.12
coord: 86..107
score: 23.22
coord: 358..376
score: 29.36
coord: 315..341
score: 33.2
coord: 398..422
score: 39.85
coord: 446..469
score: 27.53
coord: 180..198
score: 21.66
coord: 436..446
score: 55.9
coord: 62..81
score: 36.34
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 21..499
e-value: 3.1E-116
score: 390.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..508
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 36..469
e-value: 4.1E-34
score: 115.3
NoneNo IPR availablePANTHERPTHR24298:SF59CYTOCHROME P450, FAMILY 705, SUBFAMILY A, POLYPEPTIDE 25-RELATEDcoord: 6..512
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 6..512
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 439..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12141.T1Cucsat.G12141.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen