Cucsat.G1189 (gene) Cucumber (B10) v3

Overview
NameCucsat.G1189
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPhosphoenolpyruvate carboxylase kinase 4
Locationctg1: 9270299 .. 9271985 (+)
RNA-Seq ExpressionCucsat.G1189
SyntenyCucsat.G1189
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAGGTATGTTATCAAAGTTTTAGGTATGCATGTGCAATAAATGTAAAACAAAACGTACCCGTGGGAGGTACCTTTTGTTTAATGGATTATGGCCAATTATGAAATCTTGTGCAGGTCCACACAAGAATGACAAAAATTGGGAGGAGACAAGAATGACAAAAATTGGTAGGGGAAGAAAAATAAATGAGGTGAGAGCTATAGAACCGAAAAGGATGGATTATAGAGAGAATATATATAAATATATAAACCAGTACGAAAAATAATTGAAATAGTCGTAAATATAGAGATGAGATTTAAAGTATTATCAGAAATAACAATAATTTGAAAAATTGCAAACCTAACAAAATTTATCATAATAGATAGACAATGTTTTAAATATTGAATTAGATAGACATGGAAGTATATTGTTAATAGACTTTGCTATATTTCCACCCTAAAACGGTCATCAACCACTAACTATTCAAATTAATTTATTGATTTTGTTGAAAGTTTTAACTGTTGACTGACCTTACTCTTGGTTTTCTTCGGGAAAGTCTTCGTTAGTTCGTTTAGTATATGGAGAAATACTTGGGTCAAGAAGAAGAAATTAGTTTAGCTTTCCTCAAATAATAATAATATTTTGATTATATAGTTATTCTTACTTTATTTGAAAGTTATAAAATTTAGAAAATTACTTTCATACCCCTGAAATTTTGAACAACAGAATTATTATTTTTTAAAAGATTTTAAAACCTATCTTTTTATTTTATTTTTGTGAATATTTGAGATTGTACCTTAATCTTAAGATTTTTAAAAACGTATTTAAAACATTACTAAAATGATTTTATATTATTATTTTAAATAATTATGACATTTTAAGTGAGCAAAATAATTTTAGAGAAAAACTATTGTTTTAAGAGGTTTTTTTTAAAAAAAAAATAATAAAATATTTATTCTCCATGTAAAAAAGGATGAAATTACGAAAATCATCAATTTTGCTACTTTTGATAATTCTATTTTTTAACATTTATTTTGAACATTTTAATGGTCAAATTACTGTTTAGAATAATAAGGAAAAATTTTATTTCACAGATCTCGTTAATTTATTTAAAAAGATTTAAAGACTAAAAGTATATACTATGACTAGAAAGACGCACTGTGAAAATTGATGAACTAAAAATGCATCTTTTCTTTTTAAAAATTGTGACCAAAAGATTATATTTTTAAAAAAATTGAGGACGAAACACACGTTATCCAAAATTGAAGACAAGTAGATAATTGTTCTTAAAATATATTTCATGTTTCTCCACTTTATTCCTTAATTAATATCCCTCCAAACTCCCAACTCTCAAGTCCCAATCATATTCTTGTTAATTAAGAAAAAGTAAAAAGCTGCTTCCAATTTTGTCAGTATTCTCAGATCAGAATCAAATATCTTGTTCCACAACTTCGAGATTATTGTTATTGTTGATATACCTATTGTTATTTTCATTGGATCATATCTTGGTTGTTATTTTGATTGGATCATATCTCAAGATATGGTCTTTGAAATGTAACCTAATTAATTTTGTGTGTGATAGATCAAAAATTAAGTCATTCAATTAAAAGTTTTTGGTAGCATATAAAAAGTTTGATAAAAAATCGACCGATCAATCAATTGTTGATTTTTACTCCTCCATGATCGATATCTGTTTAGTAAAAAAAAAATTATTTTGATTGTGTGAATGTAATAAAGAAATTTTGGATTTCTTTGTGTAGTACTTATTTTGAGAATGAGTGAAGTTTTAAACATTAAACTTTTCAGAGAGTATGCTAATAAACTCTCAAAATTTTCTTTTGGTTTCTCAATTCTTGTAAGGTTGGTTGTTGTTTGTTTCTCTTAATTTTGTGAGTTACTCCAACAAGTTATCTCATTTGTTTGGCTTTTCGTGTCCTAAACTCTAATTCCCCTTCTTTTGATTCGATACTTTTCTCCCTTGACTATTGTGTTTAGCTTAAATCCATTTTGCCTTCTTAATAATATGATTCTTTTCTTTCAAAAGAAAATGTCTACGATGGTATAACTTAATTCTGTCCTATAATATTTCATCAAAATTAGTAAAACTCAATAGATATTATTTATTTAAAAATTAGATATTCATACGAAAAAAAATCAATACAATATTAAAAACAATACAATATATAACAATCTTTAGTTCAACTTTTCGATTATTTAATTTTAAAAATAAATCAATTTTAAAAAAATTAAAATATTTAACATCACCCAAAATAACATTTGAAACACTAATCTTTCGAAAATTTTAACATCAATATTCTTTTAAAAAATATACATGTTTTTAGTCTATTCAAACACACCTTGTTTGATGGTGAATGATCCAAGAAAAGGAAAGTTTTTTGGGGAGTGATGTGGAATGAGATTCAAAAGATATTTTGCAATCAATATAAATACTTATATATATAATTTAATTAATAATATTATTTAGATAATGTAAATTGTAAATTGCAATAATATAGGTTTTCAAGAAAAAGGGAACACGTTGATGATGGAACGCGGGACTCACCGGCATGACCAACACGCGTTCCTGTTTTCCTTCATAACGCAGTTTCTCCCCTCTTCCATAAACGACAACCTTTTTAACAAGAATATGGAAACGACGTGTCGTCCGGAATCAGTGGACCATTTCTTTTTTGGAAAGTTTCATCTGCTTACAAAACTAATAAAATTTATCCCATAAATGTTCCAAATGCAACCAAAACTAGTTTACAATCATATAATTGTGGAGATATTTGGAAGGTCGGATGTTTTAATAATAAAAATTTCTCTTTTTACGGATAATATTTAAAATGAAAAGAAGTTTAGTTCAACAATAACCGTATGTATAACTTTTAAGATAGGTGATATTTTAATTGTCGATTTGTATTTTGGTTGAGATCGTGTATTAAGTGTATTTATTTGCATTAAGGATGCATGTTGAAATTTTTGTACTGCCTTTTTAAGTACATGAGTTTGGTCAAAATTGAATGCTCATTATAGACTGTGTAACATAGTTGTTGATATTAGATGATGAGTTAATTGAACATAATTTTCCGATAGCTAAAACAATTATGAGAATTAAAAAATTGTATATATTTGCTTAAATTTAATACTGAAACTCTAGGAATAAAAAACAATAATTGCCTAAATTTAGATATTGAGTAAAAAACAATAATTTCTGTAGATTTAAATTTCAGGCGTATTTTCTTTTTCACTTTGTTTAATTCTTTAAACAATTAATTTTAATAATATCTACAATTTTACATCAAATTTAAATTATTTAAAGTGCCAAATGTGTAGATTGAAAATAACAATTAAAAGACATAAATTTTATTTGAAATATCTAAACTGGAAATTCGAAATTAGTAAAAAAAAGAAAAGAAAAAAGTATGAAAGTGAGGTATTTAAAATCGTTTTTTTTTCCCTTTGAATTAGAAAAAATATAAATTGAAAAAGAACGGAACGGAAGAAAGCGTCGTTGAAGGACACGGAAAGGGCAACGGAGAACAGAGAGGAAGGGTAATGTGGTAATTTAAAGGATGAAGGAGTCTGTTTCCTATTCAACGGCTAAAAGTATCTAAGAGTACGGGTTTTGTGATTGACCAACGTGGCGGACTCTCATTGGGTAGTTACAAAAACGAATATTCCATTTCAACATTCCGAAAAAAATGAATTCCTCCTCACTTCACGTTTTTTACTTTCTTCCATAAATATTCAACCAAACCAAACGCTTTCTAATTAGGTTCCGTACAGTTCCACACTCGAAAACCAAAAAGAGAGGAAAGCAAAAGAAAAACAGAGTAATGAGCGACGGATTTAAGAGAGACTATCAAGTAATTGAAGAGCTTGGCCGGGGAAGATTTGGTACCGTTTTCCGATGTATTTCTAAATCTTGCGGTGGAAACTACGCCGTCAAAATCATCGACAAGCGTCGGATCTCGGCCGGTGATTCACTCGACGCGGAATGTCTTGTTAACGAGACGAAGATTCTTCATCTCCTTTACCCTCACCCTCACATTCTCGCGTTGCATAATCTCTACGAAGACGAATCTCACCTTCATATGGTTCTTGATCTTTGTTCTTCTTCCGATCTCCACCGTCGAATCACACTTCAGGTTTTTTCCGAAGCTGAGGCGGCTAGGATTATGTCACAACTGATGCACGCGGTGGCTCATTGTCACCGTCACGGCGTTGCGCATCGTGATATTAAACCAGACAATATTCTGTTCGACGAGTGGGATTCGGTGAAATTGGCGGATTTCGGATCGACGGAAATGTTTAAGCAGGGAGAAGAGTCGATGAGCGGTGTCGTCGGAACGCCGTACTACGTAGCGCCAGAAGTTCTAGCCGGAAAAGACTACGGCGAAAAGGTTGACGTCTGGAGCGCCGGCGTGGTTTTGTATGTTATGTTAGCTGGTTTTCCTCCGTTTCATGGTGAATCGGTTGTTGAGATCTTTCACGCGGTGTTACGAGCGAATTTGAGATTTCCGTCCAGAGTTTTCCATTCTGTATCTCCCTCGGCAAAAGACTTGCTTCGTAAAATGCTATGTAAAGACGTTTCCAGAAGAATTTCCGCGGAACAAGTCCTCAGTGAGTATATAGCTATTTCCCTTCAATTATCTCTAAATATTTCCCTTTTCGCCGTCTTTTATCAATCTTCCACTGAACACCATTCTTCTTCATCTCGAAAAATCAAACCCTCTTCAAAAAAAAAAAAAAAAAACACGAAACCCCTAATTTGTGCCCTGAAACAGAATTAATTCCTTTAAACCACCCCAAATCTTTGAGATCTTATCAATGTATTCGATTCAAATACACTACGCAAACAAATCCAAGCCTTAATTTAGTTTGATTTGATATATATATATATATAATATTATGTAGCATTATATTATTTTCTTCCGCTTAGTAATTCTTCCGTTTTTTGTTTGGCGGAATCAGGACATCCATGGATAACAAGGTACGCAGAAAACATGGGAGAAACGGAAGTAGGGTGAAATTTTGCAATAATTGAAGGAAGAAAAGAAGAGAATATATTATGATTGAGCCTCATCTTCTTCCATTTTCTTTTTTGTATAGAGGGTTTCGACAGTTGTAATTATAATGAGGGTATTATGATATATAAAGCAGTTTAATTAGTGAAAGAGTTTGTATAAACTGTTTCAAACTTCTCCGTTGATTTCTATATGCAAATGGGTGGCCAGAGTTTTATGTCCATATATGCTTTCTACTCTTTCTCCTGCCTTCTCGATATCAAAAGGTAAACATAAGAGAGGATTTCATATTTTATTTGTTGAAATGAGTTTTAATTTAA

Coding sequence (CDS)

AGGTTCCGTACAGTTCCACACTCGAAAACCAAAAAGAGAGGAAAGCAAAAGAAAAACAGAGTAATGAGCGACGGATTTAAGAGAGACTATCAAGTAATTGAAGAGCTTGGCCGGGGAAGATTTGGTACCGTTTTCCGATGTATTTCTAAATCTTGCGGTGGAAACTACGCCGTCAAAATCATCGACAAGCGTCGGATCTCGGCCGGTGATTCACTCGACGCGGAATGTCTTGTTAACGAGACGAAGATTCTTCATCTCCTTTACCCTCACCCTCACATTCTCGCGTTGCATAATCTCTACGAAGACGAATCTCACCTTCATATGGTTCTTGATCTTTGTTCTTCTTCCGATCTCCACCGTCGAATCACACTTCAGGTTTTTTCCGAAGCTGAGGCGGCTAGGATTATGTCACAACTGATGCACGCGGTGGCTCATTGTCACCGTCACGGCGTTGCGCATCGTGATATTAAACCAGACAATATTCTGTTCGACGAGTGGGATTCGGTGAAATTGGCGGATTTCGGATCGACGGAAATGTTTAAGCAGGGAGAAGAGTCGATGAGCGGTGTCGTCGGAACGCCGTACTACGTAGCGCCAGAAGTTCTAGCCGGAAAAGACTACGGCGAAAAGGTTGACGTCTGGAGCGCCGGCGTGGTTTTGTATGTTATGTTAGCTGGTTTTCCTCCGTTTCATGGTGAATCGGTTGTTGAGATCTTTCACGCGGTGTTACGAGCGAATTTGAGATTTCCGTCCAGAGTTTTCCATTCTGTATCTCCCTCGGCAAAAGACTTGCTTCGTAAAATGCTATGTAAAGACGTTTCCAGAAGAATTTCCGCGGAACAAGTCCTCAGACATCCATGGATAACAAGGTACGCAGAAAACATGGGAGAAACGGAAGTAGGGTGA

Protein sequence

RFRTVPHSKTKKRGKQKKNRVMSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMHAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG
Homology
BLAST of Cucsat.G1189 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 5.3e-76
Identity = 151/273 (55.31%), Postives = 189/273 (69.23%), Query Frame = 0

Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           YQ+ EE+GRGRFGTV R  + + G  +A K IDK  +S  D LD  CL NE K++ LL  
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLS--DDLDRACLDNEPKLMALLSY 74

Query: 69  HPHILALHNLYEDESHLHMVLDLC--SSSDLHRRITLQVFSEAEAARIMSQLMHAVAHCH 128
           HP+I+ +H+L + +S L + ++L   S S   R ++   F E + A    Q++ A++HCH
Sbjct: 75  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 134

Query: 129 RHGVAHRDIKPDNILFD-EWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGKD 188
           R+GV HRDIKP+NIL D   D+VK+ DFGS     +G E+  GVVGTPYYVAPEVL G  
Sbjct: 135 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYS 194

Query: 189 YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLLR 248
           YGEKVD+WSAGVVLY MLAG PPF+GE+  EIF AVLR NLRFP+++F  VS  AKD LR
Sbjct: 195 YGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLR 254

Query: 249 KMLCKDVSRRISAEQVLRHPWITRYAENMGETE 279
           K++CKD SRR SAEQ LRHPWI R     GETE
Sbjct: 255 KLICKDASRRFSAEQALRHPWIQR----AGETE 280

BLAST of Cucsat.G1189 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 2.5e-70
Identity = 139/266 (52.26%), Postives = 183/266 (68.80%), Query Frame = 0

Query: 6   KRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHL 65
           + +YQ+ +E+GRGRFGT+ RC S +    YA K IDKR +   D+LD EC+  E +I+ +
Sbjct: 8   ENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLI--DALDRECIETEPRIMAM 67

Query: 66  LYPHPHILALHNLYEDESHLHMVLDLCS-SSDLHRRITLQ--VFSEAEAARIMSQLMHAV 125
           L PHP+I+ + +LYE E  L +V++L      ++ R+       SE+E+A    Q++ A+
Sbjct: 68  LPPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSAL 127

Query: 126 AHCHRHGVAHRDIKPDNILFD-EWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVL 185
           AHCHR  V HRD+KPDN+L D     VKL DFGS      G E+  GVVGTPYYVAPEV+
Sbjct: 128 AHCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWL--GGETAEGVVGTPYYVAPEVV 187

Query: 186 AGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAK 245
            G+ Y EKVD+WSAGVV+Y MLAG PPF+GE+  +IF ++LR NLRFP + F SVS  AK
Sbjct: 188 MGRKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAK 247

Query: 246 DLLRKMLCKDVSRRISAEQVLRHPWI 268
           DLLRKM+C+DVSRR SAE  LRH W+
Sbjct: 248 DLLRKMICRDVSRRFSAEDALRHSWM 269

BLAST of Cucsat.G1189 vs. ExPASy Swiss-Prot
Match: P28583 (Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.5e-57
Identity = 119/263 (45.25%), Postives = 169/263 (64.26%), Query Frame = 0

Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           Y+V  +LG+G+FGT F C  ++ GG +A K I KR++   +  D E +  E +I+H L  
Sbjct: 34  YEVGRKLGQGQFGTTFECTRRASGGKFACKSIPKRKLLCKE--DYEDVWREIQIMHHLSE 93

Query: 69  HPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ-VFSEAEAARIMSQLMHAVAHCHR 128
           H +++ +   YED + +H+V++LC   +L  RI  +  +SE +AAR++  ++  V  CH 
Sbjct: 94  HANVVRIEGTYEDSTAVHLVMELCEGGELFDRIVQKGHYSERQAARLIKTIVEVVEACHS 153

Query: 129 HGVAHRDIKPDNILFDEWD---SVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGK 188
            GV HRD+KP+N LFD  D    +K  DFG +  +K G ES   VVG+PYYVAPEVL  K
Sbjct: 154 LGVMHRDLKPENFLFDTIDEDAKLKATDFGLSVFYKPG-ESFCDVVGSPYYVAPEVLR-K 213

Query: 189 DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLL 248
            YG + DVWSAGV+LY++L+G PPF  ES   IF  +L   L F S  + S+S SAKDL+
Sbjct: 214 LYGPESDVWSAGVILYILLSGVPPFWAESEPGIFRQILLGKLDFHSEPWPSISDSAKDLI 273

Query: 249 RKMLCKDVSRRISAEQVLRHPWI 268
           RKML ++   R++A +VLRHPWI
Sbjct: 274 RKMLDQNPKTRLTAHEVLRHPWI 292

BLAST of Cucsat.G1189 vs. ExPASy Swiss-Prot
Match: Q53P85 (Calcium-dependent protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK24 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.5e-57
Identity = 115/263 (43.73%), Postives = 172/263 (65.40%), Query Frame = 0

Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           Y++ ++LG+G+FGT + C+ K+ GG +A K I KR++   +  D E +  E +I+H L  
Sbjct: 47  YRIGKKLGQGQFGTTYLCVDKASGGEFACKSIPKRKLLCRE--DYEDVWREIQIMHHLSE 106

Query: 69  HPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ-VFSEAEAARIMSQLMHAVAHCHR 128
           HP+++ +   YED   +H+V++LC+  +L  RI  +  ++E  AA+++  ++  V  CH 
Sbjct: 107 HPNVVRIRGAYEDALFVHIVMELCAGGELFDRIVAKGHYTERAAAQLIRTIVAVVEGCHS 166

Query: 129 HGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGK 188
            GV HRD+KP+N LF    E   +K  DFG +  +K G++  S VVG+PYYVAPEVL  K
Sbjct: 167 LGVMHRDLKPENFLFASAAEDAPLKATDFGLSMFYKPGDK-FSDVVGSPYYVAPEVLQ-K 226

Query: 189 DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLL 248
            YG + DVWSAGV+LY++L G PPF  E+   IF  +LR  L F S  + S+S SAKDL+
Sbjct: 227 CYGPESDVWSAGVILYILLCGVPPFWAETEAGIFRQILRGKLDFESEPWPSISDSAKDLV 286

Query: 249 RKMLCKDVSRRISAEQVLRHPWI 268
           R MLC+D ++R++A +VL HPWI
Sbjct: 287 RNMLCRDPTKRLTAHEVLCHPWI 305

BLAST of Cucsat.G1189 vs. ExPASy Swiss-Prot
Match: Q38870 (Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CPK2 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 9.3e-57
Identity = 118/270 (43.70%), Postives = 172/270 (63.70%), Query Frame = 0

Query: 2   SDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETK 61
           ++ FK  Y +  +LG+G+FGT F C+ K  G  YA K I KR++   +  D E +  E +
Sbjct: 179 TENFKEFYSLGRKLGQGQFGTTFLCLEKGTGNEYACKSISKRKLLTDE--DVEDVRREIQ 238

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ-VFSEAEAARIMSQLMH 121
           I+H L  HP+++++   YED   +H+V++LCS  +L  RI  +  ++E +AA +   ++ 
Sbjct: 239 IMHHLAGHPNVISIKGAYEDVVAVHLVMELCSGGELFDRIIQRGHYTERKAAELARTIVG 298

Query: 122 AVAHCHRHGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVA 181
            +  CH  GV HRD+KP+N LF   +E   +K  DFG +  FK  +E  + VVG+PYYVA
Sbjct: 299 VLEACHSLGVMHRDLKPENFLFVSREEDSLLKTIDFGLSMFFKP-DEVFTDVVGSPYYVA 358

Query: 182 PEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  K YG + DVWSAGV++Y++L+G PPF  E+   IF  VL  +L F S  + S+S
Sbjct: 359 PEVLR-KRYGPESDVWSAGVIVYILLSGVPPFWAETEQGIFEQVLHGDLDFSSDPWPSIS 418

Query: 242 PSAKDLLRKMLCKDVSRRISAEQVLRHPWI 268
            SAKDL+RKML +D  RR++A QVL HPW+
Sbjct: 419 ESAKDLVRKMLVRDPKRRLTAHQVLCHPWV 444

BLAST of Cucsat.G1189 vs. NCBI nr
Match: XP_031739464.1 (phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus] >KGN56859.1 hypothetical protein Csa_009620 [Cucumis sativus])

HSP 1 Score: 570 bits (1469), Expect = 3.56e-205
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG 280
           KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG 280

BLAST of Cucsat.G1189 vs. NCBI nr
Match: XP_008438527.1 (PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo] >TYK17304.1 phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 552 bits (1423), Expect = 3.68e-198
Identity = 270/280 (96.43%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFG VFRCIS+S GGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ+FSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGS EMFKQGEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG 280
           KDLLRKMLCKDVSRRISAEQVLRHPWIT Y ENM ETE+G
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITNYVENMAETELG 280

BLAST of Cucsat.G1189 vs. NCBI nr
Match: XP_038900016.1 (phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida])

HSP 1 Score: 526 bits (1354), Expect = 1.21e-187
Identity = 259/279 (92.83%), Postives = 264/279 (94.62%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEE+GRGRFGTVFRC S+S GG YAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEEIGRGRFGTVFRCTSRSSGGIYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KI+HLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITL+VFSE EAA IMSQLMH
Sbjct: 61  KIIHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLRVFSEVEAATIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGS EMFK GEESMSGVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKHGEESMSGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGK YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP A
Sbjct: 181 LAGKKYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPPA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEV 279
           KDLLRKMLCKDVSRRISAEQVLRHPWIT   EN   TE+
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITSCVENRPATEL 279

BLAST of Cucsat.G1189 vs. NCBI nr
Match: KAA0036146.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 520 bits (1338), Expect = 1.20e-184
Identity = 256/262 (97.71%), Postives = 259/262 (98.85%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFG VFRCIS+S GGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ+FSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGS EMFKQGEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVL 262
           KDLLRKMLCKDVSRRISAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Cucsat.G1189 vs. NCBI nr
Match: XP_022924623.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata])

HSP 1 Score: 513 bits (1322), Expect = 9.14e-183
Identity = 253/277 (91.34%), Postives = 259/277 (93.50%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSD  KRDYQVIEE+GRGRFGTVFRC S+S G NYAVK IDKRRI AGDSLDAECLV ET
Sbjct: 1   MSDDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVKET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDE HLHMVLDLCSSSDLHRR+TLQVFSEAEAA IMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHR+GVAHRDIKPDNILFDEWDSVKLADFGS EMFKQGEESM GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGET 277
           KDLLRKMLCKDVSRRISAEQVLRHPWIT   E+   T
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITSCVESRAAT 277

BLAST of Cucsat.G1189 vs. ExPASy TrEMBL
Match: A0A0A0L743 (Phosphoenolpyruvate carboxylase kinase 4 OS=Cucumis sativus OX=3659 GN=Csa_3G135070 PE=3 SV=1)

HSP 1 Score: 570 bits (1469), Expect = 1.73e-205
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG 280
           KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG 280

BLAST of Cucsat.G1189 vs. ExPASy TrEMBL
Match: A0A5D3D228 (Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001580 PE=3 SV=1)

HSP 1 Score: 552 bits (1423), Expect = 1.78e-198
Identity = 270/280 (96.43%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFG VFRCIS+S GGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ+FSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGS EMFKQGEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG 280
           KDLLRKMLCKDVSRRISAEQVLRHPWIT Y ENM ETE+G
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITNYVENMAETELG 280

BLAST of Cucsat.G1189 vs. ExPASy TrEMBL
Match: A0A1S3AWP8 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo OX=3656 GN=LOC103483595 PE=3 SV=1)

HSP 1 Score: 552 bits (1423), Expect = 1.78e-198
Identity = 270/280 (96.43%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFG VFRCIS+S GGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ+FSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGS EMFKQGEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGETEVG 280
           KDLLRKMLCKDVSRRISAEQVLRHPWIT Y ENM ETE+G
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITNYVENMAETELG 280

BLAST of Cucsat.G1189 vs. ExPASy TrEMBL
Match: A0A5A7T1U4 (Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1463G00010 PE=3 SV=1)

HSP 1 Score: 520 bits (1338), Expect = 5.81e-185
Identity = 256/262 (97.71%), Postives = 259/262 (98.85%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSDGFKRDYQVIEELGRGRFG VFRCIS+S GGNYAVKIIDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ+FSEAEAARIMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGS EMFKQGEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVL 262
           KDLLRKMLCKDVSRRISAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Cucsat.G1189 vs. ExPASy TrEMBL
Match: A0A6J1ED04 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111432056 PE=3 SV=1)

HSP 1 Score: 513 bits (1322), Expect = 4.42e-183
Identity = 253/277 (91.34%), Postives = 259/277 (93.50%), Query Frame = 0

Query: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60
           MSD  KRDYQVIEE+GRGRFGTVFRC S+S G NYAVK IDKRRI AGDSLDAECLV ET
Sbjct: 1   MSDDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVKET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120
           KILHLLYPHPHILALHNLYEDE HLHMVLDLCSSSDLHRR+TLQVFSEAEAA IMSQLMH
Sbjct: 61  KILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIMSQLMH 120

Query: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180
           AVAHCHR+GVAHRDIKPDNILFDEWDSVKLADFGS EMFKQGEESM GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYYVAPEV 180

Query: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240
           LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240

Query: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITRYAENMGET 277
           KDLLRKMLCKDVSRRISAEQVLRHPWIT   E+   T
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLRHPWITSCVESRAAT 277

BLAST of Cucsat.G1189 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 285.8 bits (730), Expect = 3.7e-77
Identity = 151/273 (55.31%), Postives = 189/273 (69.23%), Query Frame = 0

Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           YQ+ EE+GRGRFGTV R  + + G  +A K IDK  +S  D LD  CL NE K++ LL  
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLS--DDLDRACLDNEPKLMALLSY 74

Query: 69  HPHILALHNLYEDESHLHMVLDLC--SSSDLHRRITLQVFSEAEAARIMSQLMHAVAHCH 128
           HP+I+ +H+L + +S L + ++L   S S   R ++   F E + A    Q++ A++HCH
Sbjct: 75  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 134

Query: 129 RHGVAHRDIKPDNILFD-EWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGKD 188
           R+GV HRDIKP+NIL D   D+VK+ DFGS     +G E+  GVVGTPYYVAPEVL G  
Sbjct: 135 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYS 194

Query: 189 YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLLR 248
           YGEKVD+WSAGVVLY MLAG PPF+GE+  EIF AVLR NLRFP+++F  VS  AKD LR
Sbjct: 195 YGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLR 254

Query: 249 KMLCKDVSRRISAEQVLRHPWITRYAENMGETE 279
           K++CKD SRR SAEQ LRHPWI R     GETE
Sbjct: 255 KLICKDASRRFSAEQALRHPWIQR----AGETE 280

BLAST of Cucsat.G1189 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 268.5 bits (685), Expect = 6.2e-72
Identity = 141/257 (54.86%), Postives = 179/257 (69.65%), Query Frame = 0

Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           YQ+ EE+GRGRFGTV R  + + G  +A K IDK  +S  D LD  CL NE K++ LL  
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLS--DDLDRACLDNEPKLMALLSY 74

Query: 69  HPHILALHNLYEDESHLHMVLDLC--SSSDLHRRITLQVFSEAEAARIMSQLMHAVAHCH 128
           HP+I+ +H+L + +S L + ++L   S S   R ++   F E + A    Q++ A++HCH
Sbjct: 75  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 134

Query: 129 RHGVAHRDIKPDNILFD-EWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGKD 188
           R+GV HRDIKP+NIL D   D+VK+ DFGS     +G E+  GVVGTPYYVAPEVL G  
Sbjct: 135 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYS 194

Query: 189 YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLLR 248
           YGEKVD+WSAGVVLY MLAG PPF+GE+  EIF AVLR NLRFP+++F  VS  AKD LR
Sbjct: 195 YGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLR 254

Query: 249 KMLCKDVSRRISAEQVL 263
           K++CKD SRR SAEQ L
Sbjct: 255 KLICKDASRRFSAEQAL 268

BLAST of Cucsat.G1189 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 266.9 bits (681), Expect = 1.8e-71
Identity = 139/266 (52.26%), Postives = 183/266 (68.80%), Query Frame = 0

Query: 6   KRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHL 65
           + +YQ+ +E+GRGRFGT+ RC S +    YA K IDKR +   D+LD EC+  E +I+ +
Sbjct: 8   ENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLI--DALDRECIETEPRIMAM 67

Query: 66  LYPHPHILALHNLYEDESHLHMVLDLCS-SSDLHRRITLQ--VFSEAEAARIMSQLMHAV 125
           L PHP+I+ + +LYE E  L +V++L      ++ R+       SE+E+A    Q++ A+
Sbjct: 68  LPPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSAL 127

Query: 126 AHCHRHGVAHRDIKPDNILFD-EWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVL 185
           AHCHR  V HRD+KPDN+L D     VKL DFGS      G E+  GVVGTPYYVAPEV+
Sbjct: 128 AHCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWL--GGETAEGVVGTPYYVAPEVV 187

Query: 186 AGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAK 245
            G+ Y EKVD+WSAGVV+Y MLAG PPF+GE+  +IF ++LR NLRFP + F SVS  AK
Sbjct: 188 MGRKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAK 247

Query: 246 DLLRKMLCKDVSRRISAEQVLRHPWI 268
           DLLRKM+C+DVSRR SAE  LRH W+
Sbjct: 248 DLLRKMICRDVSRRFSAEDALRHSWM 269

BLAST of Cucsat.G1189 vs. TAIR 10
Match: AT3G10660.1 (calmodulin-domain protein kinase cdpk isoform 2 )

HSP 1 Score: 221.9 bits (564), Expect = 6.6e-58
Identity = 118/270 (43.70%), Postives = 172/270 (63.70%), Query Frame = 0

Query: 2   SDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETK 61
           ++ FK  Y +  +LG+G+FGT F C+ K  G  YA K I KR++   +  D E +  E +
Sbjct: 179 TENFKEFYSLGRKLGQGQFGTTFLCLEKGTGNEYACKSISKRKLLTDE--DVEDVRREIQ 238

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ-VFSEAEAARIMSQLMH 121
           I+H L  HP+++++   YED   +H+V++LCS  +L  RI  +  ++E +AA +   ++ 
Sbjct: 239 IMHHLAGHPNVISIKGAYEDVVAVHLVMELCSGGELFDRIIQRGHYTERKAAELARTIVG 298

Query: 122 AVAHCHRHGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVA 181
            +  CH  GV HRD+KP+N LF   +E   +K  DFG +  FK  +E  + VVG+PYYVA
Sbjct: 299 VLEACHSLGVMHRDLKPENFLFVSREEDSLLKTIDFGLSMFFKP-DEVFTDVVGSPYYVA 358

Query: 182 PEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  K YG + DVWSAGV++Y++L+G PPF  E+   IF  VL  +L F S  + S+S
Sbjct: 359 PEVLR-KRYGPESDVWSAGVIVYILLSGVPPFWAETEQGIFEQVLHGDLDFSSDPWPSIS 418

Query: 242 PSAKDLLRKMLCKDVSRRISAEQVLRHPWI 268
            SAKDL+RKML +D  RR++A QVL HPW+
Sbjct: 419 ESAKDLVRKMLVRDPKRRLTAHQVLCHPWV 444

BLAST of Cucsat.G1189 vs. TAIR 10
Match: AT1G76040.2 (calcium-dependent protein kinase 29 )

HSP 1 Score: 214.9 bits (546), Expect = 8.1e-56
Identity = 115/264 (43.56%), Postives = 167/264 (63.26%), Query Frame = 0

Query: 9   YQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNETKILHLLYP 68
           Y + +ELGRG+FG  ++C  KS G  YA K I KR++      D E +  E  IL  L  
Sbjct: 112 YDLHKELGRGQFGITYKCTDKSNGREYACKSISKRKLIR--RKDIEDVRREVMILQHLTG 171

Query: 69  HPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQ-VFSEAEAARIMSQLMHAVAHCHR 128
            P+I+     YED+ +LH+V++LCS  +L  RI  +  +SE EAA I  Q+++ V  CH 
Sbjct: 172 QPNIVEFRGAYEDKDNLHLVMELCSGGELFDRIIKKGSYSEKEAANIFRQIVNVVHVCHF 231

Query: 129 HGVAHRDIKPDNILF---DEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEVLAGK 188
            GV HRD+KP+N L    +E   +K  DFG +   ++G +    +VG+ YYVAPEVL  +
Sbjct: 232 MGVVHRDLKPENFLLVSNEEDSPIKATDFGLSVFIEEG-KVYRDIVGSAYYVAPEVL-HR 291

Query: 189 DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSAKDLL 248
           +YG+++DVWSAGV+LY++L+G PPF GE+   IF A+L   L   +  + ++S SAKDL+
Sbjct: 292 NYGKEIDVWSAGVMLYILLSGVPPFWGETEKTIFEAILEGKLDLETSPWPTISESAKDLI 351

Query: 249 RKMLCKDVSRRISAEQVLRHPWIT 269
           RKML +D  +RI+A + L HPW+T
Sbjct: 352 RKMLIRDPKKRITAAEALEHPWMT 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SPK45.3e-7655.31Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK02.5e-7052.26Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
P285832.5e-5745.25Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1[more]
Q53P855.5e-5743.73Calcium-dependent protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q388709.3e-5743.70Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CPK2 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_031739464.13.56e-205100.00phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus] >KGN56859.1 hypotheti... [more]
XP_008438527.13.68e-19896.43PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo] >TYK1730... [more]
XP_038900016.11.21e-18792.83phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida][more]
KAA0036146.11.20e-18497.71phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo var. makuwa][more]
XP_022924623.19.14e-18391.34phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0L7431.73e-205100.00Phosphoenolpyruvate carboxylase kinase 4 OS=Cucumis sativus OX=3659 GN=Csa_3G135... [more]
A0A5D3D2281.78e-19896.43Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3AWP81.78e-19896.43phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5A7T1U45.81e-18597.71Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1ED044.42e-18391.34phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G08650.13.7e-7755.31phosphoenolpyruvate carboxylase kinase 1 [more]
AT1G08650.26.2e-7254.86phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.11.8e-7152.26phosphoenolpyruvate carboxylase kinase 2 [more]
AT3G10660.16.6e-5843.70calmodulin-domain protein kinase cdpk isoform 2 [more]
AT1G76040.28.1e-5643.56calcium-dependent protein kinase 29 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 30..288
e-value: 1.3E-98
score: 343.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 30..288
e-value: 3.8E-74
score: 249.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 30..288
score: 50.604679
NoneNo IPR availablePIRSFPIRSF000654ILKcoord: 3..286
e-value: 1.5E-24
score: 84.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 3..114
e-value: 1.0E-24
score: 88.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 116..300
e-value: 2.0E-64
score: 219.3
NoneNo IPR availablePIRSRPIRSR630616-1PIRSR630616-1coord: 11..296
e-value: 3.6E-58
score: 194.9
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 33..244
e-value: 7.6E-25
score: 85.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 25..294
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 25..294
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 29..287
e-value: 2.04694E-121
score: 346.386
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 36..59
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 151..163
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 26..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G1189.T2Cucsat.G1189.T2mRNA
Cucsat.G1189.T1Cucsat.G1189.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity