Cucsat.G11887 (gene) Cucumber (B10) v3

Overview
NameCucsat.G11887
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationctg1820: 2471363 .. 2474875 (-)
RNA-Seq ExpressionCucsat.G11887
SyntenyCucsat.G11887
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACGTAGTGAAAAGAAAGTGGGAAAAAGAACGCAGTTGATAAAGGGTAAAATTGGAAAATAAGAAAGAATTGACAAAAATGACGAGTAAAGAAATGAGTTATCATTCGAAGAAAAATGGGGAGAGGAATTTATATATATATATATATGGAGAGAGGGTGTAGCTAGGGAGGTAAATGAGCAGTGTGATGGAGATCCAACTTTTGTGTTCAGGGGGACGTCACGAGAAGTTGCCGGAGAAGTATGAACGGCCTGAATCGGATAGGCCGCGGCTGTCGGAGGTGTGTTGTTGGGACAAGGTTCCAATAATCGACTTGGGATGCGAGGAGAGAGAGATGATTGTGAAGCAAGTGGAGGAGGCCTGCAAGTCTTACGGCTTTTTCCAGGTAAATAGTTATTCATAATTTTTGTTTGATTGGAAACTAGACCGATCATATGTAAAAATATATATTAATAAAACACAGGTTATAAATCATGGTGTGAGGAAGGAATTGGTGGAGAAAGTGATAGAAGTTGGCAAGCAGTTCTTTGAGCTGCCGATGGAGGAGAAGTTGAAATTTTATTCAGACGACCCTTCCAAGACCGTCAGACTCTCCACAAGTTTCAATGTCCGGAAAGAGCAATTTCGCAACTGGAGGGATTATCTCAGACTCCATTGCTATCCTCTCTCCAACTACACCCCCCATTGGCCCTCTAACCCACCATCCTTCAGGTTTTTTTGTTTTTTGTTTTTTTCTTTTTAACTCTCTAATTCCCTTAATATTATTATCCTAATCTATATATGCAAATTAAACATTAACTTTTCTTTTATTTTTCTATCATCCGTTAATCATCTCTAGTATTATTTTTTTGTTCTTTTTATTATTATAATAGAGCGTCTGACAGATTTTGATATACTTTTTTAAAAAATAAAGTCCGTACATAATCTATCTCTAACCGAAAAACTAACTAAAAATATATTCTTTACAAACAAAAGGCAATAATGAATATTCCATTGTATAATTTCTGTAGAAAAGACTTTTTGGATAGAATTAAATAAACGAATGAAGCAAACTATAGCATAGGTGTAGGAGGTGAGGGCAGCCATCTTTAACAAGAACTACCCTCAGTTTGAAGAATTGAGTTTAAAAAAGATCCAATAAACCAATTGGGGGCTAATTCAGTAGTATCCTGATTATTGCTACTATGACTTTTAATCTGGTGAAAAATGTTAGCCACACACACTTGAATTGAAAAACAAAATCTGGAGAAGAAGAAGGATTTTATATATCTATATATTGGAAAAAAGAGAAAAGAAAAGTAAAATGAGGAGGAAATAAAAGCGTAGATATTTTGTTGAAGTGAAGATGAAATTTAATTAATTTAATTTATGAATCTTTTTTTTTTATTTGTGTGATATGAACTTAGAATTGTGAATTGGGTTTAGGGTTGGAGAAGGACCATTGACTGGGATAGTAGTAGAAGTGGGTCCATATAGGGTGGAAATTAGAAGTAGCGTGGTTCCATGTGCGCCCGCCCGGCGCCCATTATTTCAAAATTACTAACCTTTTTTTTTATGGATCTTCTTTCTCTCTCCGTTGAAATGGACGGCGGTGACGGCACCCCCATTTCACACTCACACATCGCACAACCTCTCTCTTTTTATTTTATTTTAATACCTCATCTCTGACGTCATTGTTCAAACCGCGTTGACGTAGCTATCCTCATCATCCAACGCCCAACAATGCAGCACGCCATCTTTCATGGATCCTCCCATCCTCCTCCCACTATTTTATATTTTATACTTTTCTTTCTTTCTTTCGTTATTATTTATTTATTTTAAGCATTTCATCTCCTTTTCTTGCTTCTATGATACAGTCATTCTTTTGTCCATAATCTATTTAAATTATTCTTATTCTTATTCTTATTTAATAACTACCTTTCATTTTAAATCATTAAACCTCCTCTCTTATTTGATTATTACTATTTTCTTTAATAAAAAAAATGCACATTTAAAAATTGTAAACGTACATCACAATAAAAAATATTTTAATATTTCAAAATATTCTCCATCAACATACTTTACAAAGATCTATTTGATGAAAATTTCAACAAAAATTTAGAAAACAAAACACCATTAGATTAAAAGAAAGATACTCCTACTGTTAAAATGTTATCTCTAAACCTTAAAAAATATTTCTTTCCATAAAAGATTTAAGAATAGTGTGTATAGTCTAACTTGGGTGTGGGAGTTGGGGTTTCCATATGAGTGGTGGACCGGCAAGATAAGCAGTGGGTGGATGTAAATAAAAGTACGGACTTAAATATGAATTTGGTAGTAAAAGAAAAGAAAAAGTGATAGTGTTTTTATTGGTGCATGTATAAAATGAGAAGGAATTAATTGGATTGATATTGAATAAAGATAGATAGGAAGAATATCAATATAAATTAAAGTGTAATAAAATAGTGATGAATAATTGATGATGGGGTGGTTATGATAGGGAAATAGTGAGTAGTTATTGCAATGAAGTACGAAAAGTTGGGTACAGAATAGAGGAGCTAATATCGGAGAGCTTGGGGCTGGAGAAGGAATACATAAGGAAGAAGTTGGGTGAACAAGGTCAGCACATGGCTATAAATTATTATCCGCCATGTCCCCAACCAGAACTCACCTACGGGCTCCCTGGCCATACGGATCCCAACGCACTCACCATTCTCCTTCAGGATCTCCATGTCGCCGGCCTCCAAGTCCTCAAAGATGGAAAGTGGCTAGCGGTCAACCCCCACCCCAATGCCTTTGTAATCAATATAGGCGACCAATTGCAGGTACTTTGTACGCCTGTACGTACCTTCTACGTTTTTCAATTTAATTTTAGTTCATTTGTTTTAACAATGCCCATGTGCATACACAGGCATTGAGCAATGGGGTGTACAAGAGCGTTTGGCACCGAGCGGTGGTCAATGTTGATAAGCCCAGGCTGTCGGTCGCTTCTTTTCTCTGCCCTTGTGATGACGCCCTCATTACTCCTGCACCGCTCCTCTCCCAGCCTTCCCCCATTTACAGACCTTTCACCTACGCCCAGTACTACAATACTTTTTGGAGCAGAAACTTGGATCAACAACATTGCTTGGAACTATTTAAAAACCACCCTCCTTAAAAAAAAAACAAAAAAGATTCTCTCTCTCCATCTCTTTTATCCTTTAATTATTACGTCTTCCTAATAAAGGCAAGTGGATAAACTTTTTGGCCCAATCTGGTCTTGCCCTCTTTGTTGATGAAACGCCGACGCTTCGAACGTTGGGGGTGTATTAAATTAGAAGAATTTTTAGCACAATAGCAAAAGTTCCATTATTTACCTAAACTCTTCAACTGTTGGAATTATAGTTGAATAAGTAATTTGGACCCTTTGGTAGATTTCTTCTTTTTAGCTTTTTAATTACTAAGTACGTTTGCAAGTGATTTTTATAATTAGTTAAAATAACTTTTTATTATATTCAAAATTGTTATGAAACATCCATATATTCACTCAAAATTT

Coding sequence (CDS)

ATGAGCAGTGTGATGGAGATCCAACTTTTGTGTTCAGGGGGACGTCACGAGAAGTTGCCGGAGAAGTATGAACGGCCTGAATCGGATAGGCCGCGGCTGTCGGAGGTGTGTTGTTGGGACAAGGTTCCAATAATCGACTTGGGATGCGAGGAGAGAGAGATGATTGTGAAGCAAGTGGAGGAGGCCTGCAAGTCTTACGGCTTTTTCCAGGTTATAAATCATGGTGTGAGGAAGGAATTGGTGGAGAAAGTGATAGAAGTTGGCAAGCAGTTCTTTGAGCTGCCGATGGAGGAGAAGTTGAAATTTTATTCAGACGACCCTTCCAAGACCGTCAGACTCTCCACAAGTTTCAATGTCCGGAAAGAGCAATTTCGCAACTGGAGGGATTATCTCAGACTCCATTGCTATCCTCTCTCCAACTACACCCCCCATTGGCCCTCTAACCCACCATCCTTCAGGTTTTTTTGTTTTTTGTTTTTTTCTTTTTAA

Protein sequence

MSSVMEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACKSYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQFRNWRDYLRLHCYPLSNYTPHWPSNPPSFRFFCFLFFSF
Homology
BLAST of Cucsat.G11887 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 2.9e-50
Identity = 85/149 (57.05%), Postives = 113/149 (75.84%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           M  +L+ +G RH  LPE Y RP SDRPRLSEV   +  P+IDL   +R  +++Q+ +AC 
Sbjct: 1   MAAKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACA 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
            +GFFQVINHGV K+++++++ V ++FF + MEEK+K YSDDP+KT RLSTSFNV+KE+ 
Sbjct: 61  RFGFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEV 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 154
            NWRDYLRLHCYP+  Y   WPSNPPSF+
Sbjct: 121 NNWRDYLRLHCYPIHKYVNEWPSNPPSFK 149

BLAST of Cucsat.G11887 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 3.9e-47
Identity = 83/144 (57.64%), Postives = 105/144 (72.92%), Query Frame = 0

Query: 10  LCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACKSYGFF 69
           L S   H+ +P KY RPES RPRL  V    ++P++DL   +R  +V  V +AC+++GFF
Sbjct: 11  LLSTAVHDTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFF 70

Query: 70  QVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQFRNWRD 129
           QV+NHG+   L+  V+EVG++FF LP EEK K YSDDP+K +RLSTSFNVRKE   NWRD
Sbjct: 71  QVVNHGIDAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSFNVRKETVHNWRD 130

Query: 130 YLRLHCYPLSNYTPHWPSNPPSFR 154
           YLRLHCYPL  + P WPSNPPSF+
Sbjct: 131 YLRLHCYPLHQFVPDWPSNPPSFK 154

BLAST of Cucsat.G11887 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 2.9e-34
Identity = 71/139 (51.08%), Postives = 93/139 (66.91%), Query Frame = 0

Query: 19  LPEKYERPESDRPRLSEV-CCWDKVPII---DLGCEEREMIVKQVEEACKSYGFFQVINH 78
           +P  Y RP SDRP LSEV    D +P+I   DL    R +IV+Q+  AC +YGFFQ+ NH
Sbjct: 20  IPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNH 79

Query: 79  GVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQFRNWRDYLRLH 138
           GV    V K+  V ++FF  P  E++K YS DP+KT RLSTSFNV  ++  NWRD+LRLH
Sbjct: 80  GVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLH 139

Query: 139 CYPLSNYTPHWPSNPPSFR 154
           C+P+ ++   WPS+P SFR
Sbjct: 140 CFPIEDFIEEWPSSPISFR 158

BLAST of Cucsat.G11887 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.2e-33
Identity = 69/141 (48.94%), Postives = 91/141 (64.54%), Query Frame = 0

Query: 17  EKLPEKYERPESDRPRLSEV-CCWDKVPII---DLGCEEREMIVKQVEEACKSYGFFQVI 76
           + +P  Y RP SDRP++SEV    D +P+I   DL    R  I+ Q   AC S GFFQ+ 
Sbjct: 16  DHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIK 75

Query: 77  NHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQFRNWRDYLR 136
           NHGV +E ++K++   ++FF     E++K YS D  KT RLSTSFNV KE+  NWRD+LR
Sbjct: 76  NHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLR 135

Query: 137 LHCYPLSNYTPHWPSNPPSFR 154
           LHCYP+ ++   WPS P SFR
Sbjct: 136 LHCYPIEDFINEWPSTPISFR 156

BLAST of Cucsat.G11887 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.8e-23
Identity = 58/156 (37.18%), Postives = 88/156 (56.41%), Query Frame = 0

Query: 7   IQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWD-----KVPIIDLG----CEEREMIVK 66
           ++ LC  G   +LP  Y  P SDRP  +           K+P++DL       ER  +++
Sbjct: 41  VRHLCDSG-ITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRVPSERGAVLR 100

Query: 67  QVEEACKSYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSF 126
            ++ AC+ YGFFQV+NHGV  E+V  +++V ++FFELP  E+ ++ S D    VR  TSF
Sbjct: 101 TLDAACREYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSF 160

Query: 127 NVRKEQFRNWRDYLRLHCYPLSNYTPHWPSNPPSFR 154
           N  ++    WRD+L+L C PL+     WP++P   R
Sbjct: 161 NQVRDAVLCWRDFLKLACMPLAAVVESWPTSPADLR 195

BLAST of Cucsat.G11887 vs. NCBI nr
Match: XP_004137205.1 (protein DOWNY MILDEW RESISTANCE 6 [Cucumis sativus] >KGN53620.1 hypothetical protein Csa_014644 [Cucumis sativus])

HSP 1 Score: 328 bits (842), Expect = 8.05e-111
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MSSVMEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVE 60
           MSSVMEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVE
Sbjct: 1   MSSVMEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVE 60

Query: 61  EACKSYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVR 120
           EACKSYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVR
Sbjct: 61  EACKSYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVR 120

Query: 121 KEQFRNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
           KEQFRNWRDYLRLHCYPLSNYTPHWPSNPPSFR
Sbjct: 121 KEQFRNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153

BLAST of Cucsat.G11887 vs. NCBI nr
Match: XP_008462909.2 (PREDICTED: LOW QUALITY PROTEIN: protein DOWNY MILDEW RESISTANCE 6-like [Cucumis melo])

HSP 1 Score: 299 bits (766), Expect = 2.47e-99
Identity = 141/149 (94.63%), Postives = 143/149 (95.97%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           MEIQLLCSG RHEKLPEKYERPESDRPRLSEVC  DKVPIIDLGCEEREMIVKQVEEACK
Sbjct: 1   MEIQLLCSGERHEKLPEKYERPESDRPRLSEVCGCDKVPIIDLGCEEREMIVKQVEEACK 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
           SYGFFQVINHGVRKELVEK+IEVGK FF LP+EEKLKFYSDDPSKTVRLSTSFNVRKEQF
Sbjct: 61  SYGFFQVINHGVRKELVEKMIEVGKLFFGLPVEEKLKFYSDDPSKTVRLSTSFNVRKEQF 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
            NWRDYLRLHCYPLSNYTPHWPSNPPSFR
Sbjct: 121 HNWRDYLRLHCYPLSNYTPHWPSNPPSFR 149

BLAST of Cucsat.G11887 vs. NCBI nr
Match: XP_038895989.1 (protein DOWNY MILDEW RESISTANCE 6 [Benincasa hispida])

HSP 1 Score: 283 bits (724), Expect = 6.62e-93
Identity = 131/149 (87.92%), Postives = 137/149 (91.95%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           M+IQLLCS  RHEKLPEKYERPESDRPRL EVC WD VPIID+GCEER  IVK+V EACK
Sbjct: 1   MDIQLLCSWERHEKLPEKYERPESDRPRLWEVCDWDNVPIIDMGCEERGRIVKEVGEACK 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
           SYGFFQVINHGV KELVEK+I VGK FFELP+EEKLKFYSDDPSKTVRLSTSFNVRKEQF
Sbjct: 61  SYGFFQVINHGVSKELVEKMIGVGKHFFELPLEEKLKFYSDDPSKTVRLSTSFNVRKEQF 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
            NWRDYLRLHCYPLSNY+PHWPSNPPSFR
Sbjct: 121 HNWRDYLRLHCYPLSNYSPHWPSNPPSFR 149

BLAST of Cucsat.G11887 vs. NCBI nr
Match: XP_023552149.1 (protein DOWNY MILDEW RESISTANCE 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 259 bits (662), Expect = 1.60e-83
Identity = 119/149 (79.87%), Postives = 128/149 (85.91%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           MEIQLL SG RHE LPEKYERPESDRPRLSEVCC + VP+IDLGCE+R  +V+QV EACK
Sbjct: 1   MEIQLLSSGTRHESLPEKYERPESDRPRLSEVCCLENVPVIDLGCEDRAWMVEQVGEACK 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
            YGFFQV NHGV KELVE+ I VG  FF LP+EEKLK YSDDPSKTVRLSTSFNVRKE+F
Sbjct: 61  CYGFFQVRNHGVSKELVERTIGVGNAFFGLPLEEKLKLYSDDPSKTVRLSTSFNVRKEEF 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
            NWRDYLRLHCYPLS Y PHWP+NPP FR
Sbjct: 121 HNWRDYLRLHCYPLSKYLPHWPTNPPLFR 149

BLAST of Cucsat.G11887 vs. NCBI nr
Match: XP_022922602.1 (protein DOWNY MILDEW RESISTANCE 6 [Cucurbita moschata])

HSP 1 Score: 258 bits (659), Expect = 4.56e-83
Identity = 119/149 (79.87%), Postives = 128/149 (85.91%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           ME QLL SG RHE+LPEKYERPESDRPRLSEVCC + VPIIDLGCE+R  +V+QV EACK
Sbjct: 1   METQLLSSGTRHERLPEKYERPESDRPRLSEVCCLENVPIIDLGCEDRGWMVEQVGEACK 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
            YGFFQV NHGV KELVE+ I VG  FF LP+EEKLK YSDDPSKTVRLSTSFNVRKE+F
Sbjct: 61  CYGFFQVRNHGVSKELVERTIGVGNAFFGLPLEEKLKLYSDDPSKTVRLSTSFNVRKEEF 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
            NWRDYLRLHCYPLS Y PHWP+NPP FR
Sbjct: 121 HNWRDYLRLHCYPLSKYLPHWPTNPPLFR 149

BLAST of Cucsat.G11887 vs. ExPASy TrEMBL
Match: A0A0A0KVJ3 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis sativus OX=3659 GN=Csa_4G091870 PE=3 SV=1)

HSP 1 Score: 328 bits (842), Expect = 3.90e-111
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MSSVMEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVE 60
           MSSVMEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVE
Sbjct: 1   MSSVMEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVE 60

Query: 61  EACKSYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVR 120
           EACKSYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVR
Sbjct: 61  EACKSYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVR 120

Query: 121 KEQFRNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
           KEQFRNWRDYLRLHCYPLSNYTPHWPSNPPSFR
Sbjct: 121 KEQFRNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153

BLAST of Cucsat.G11887 vs. ExPASy TrEMBL
Match: A0A1S3CHY9 (LOW QUALITY PROTEIN: protein DOWNY MILDEW RESISTANCE 6-like OS=Cucumis melo OX=3656 GN=LOC103501178 PE=3 SV=1)

HSP 1 Score: 299 bits (766), Expect = 1.19e-99
Identity = 141/149 (94.63%), Postives = 143/149 (95.97%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           MEIQLLCSG RHEKLPEKYERPESDRPRLSEVC  DKVPIIDLGCEEREMIVKQVEEACK
Sbjct: 1   MEIQLLCSGERHEKLPEKYERPESDRPRLSEVCGCDKVPIIDLGCEEREMIVKQVEEACK 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
           SYGFFQVINHGVRKELVEK+IEVGK FF LP+EEKLKFYSDDPSKTVRLSTSFNVRKEQF
Sbjct: 61  SYGFFQVINHGVRKELVEKMIEVGKLFFGLPVEEKLKFYSDDPSKTVRLSTSFNVRKEQF 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
            NWRDYLRLHCYPLSNYTPHWPSNPPSFR
Sbjct: 121 HNWRDYLRLHCYPLSNYTPHWPSNPPSFR 149

BLAST of Cucsat.G11887 vs. ExPASy TrEMBL
Match: A0A6J1E3R8 (protein DOWNY MILDEW RESISTANCE 6 OS=Cucurbita moschata OX=3662 GN=LOC111430560 PE=3 SV=1)

HSP 1 Score: 258 bits (659), Expect = 2.21e-83
Identity = 119/149 (79.87%), Postives = 128/149 (85.91%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           ME QLL SG RHE+LPEKYERPESDRPRLSEVCC + VPIIDLGCE+R  +V+QV EACK
Sbjct: 1   METQLLSSGTRHERLPEKYERPESDRPRLSEVCCLENVPIIDLGCEDRGWMVEQVGEACK 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
            YGFFQV NHGV KELVE+ I VG  FF LP+EEKLK YSDDPSKTVRLSTSFNVRKE+F
Sbjct: 61  CYGFFQVRNHGVSKELVERTIGVGNAFFGLPLEEKLKLYSDDPSKTVRLSTSFNVRKEEF 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
            NWRDYLRLHCYPLS Y PHWP+NPP FR
Sbjct: 121 HNWRDYLRLHCYPLSKYLPHWPTNPPLFR 149

BLAST of Cucsat.G11887 vs. ExPASy TrEMBL
Match: A0A6J1JAA4 (protein DOWNY MILDEW RESISTANCE 6 OS=Cucurbita maxima OX=3661 GN=LOC111482687 PE=3 SV=1)

HSP 1 Score: 255 bits (651), Expect = 3.60e-82
Identity = 119/149 (79.87%), Postives = 126/149 (84.56%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           MEIQLL SG RHE LPEKYERPESDRPRLSEVC  + VPIIDLGCE+R  +V QV EACK
Sbjct: 1   MEIQLLSSGTRHESLPEKYERPESDRPRLSEVCSLENVPIIDLGCEDRAWMVAQVGEACK 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
            YGFFQV NHGV KELVE+ I VG  FF LP+EEKLK YSDDPSKTVRLSTSFNVRKE+F
Sbjct: 61  CYGFFQVRNHGVSKELVERTIGVGNAFFGLPLEEKLKLYSDDPSKTVRLSTSFNVRKEEF 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
            NWRDYLRLHCYPLS Y PHWP+NPP FR
Sbjct: 121 HNWRDYLRLHCYPLSKYLPHWPTNPPLFR 149

BLAST of Cucsat.G11887 vs. ExPASy TrEMBL
Match: A0A6J1CGV1 (protein DOWNY MILDEW RESISTANCE 6 OS=Momordica charantia OX=3673 GN=LOC111011401 PE=3 SV=1)

HSP 1 Score: 248 bits (633), Expect = 2.03e-79
Identity = 113/149 (75.84%), Postives = 130/149 (87.25%), Query Frame = 0

Query: 5   MEIQLLCSGGRHEKLPEKYERPESDRPRLSEVCCWDKVPIIDLGCEEREMIVKQVEEACK 64
           M+I++L SG R+E LPEKY RPES+RPRLSEV   + VPIIDLGC +R  +V++V EACK
Sbjct: 1   MDIKVLSSGVRYENLPEKYARPESERPRLSEVSACENVPIIDLGCGDRSRVVQEVGEACK 60

Query: 65  SYGFFQVINHGVRKELVEKVIEVGKQFFELPMEEKLKFYSDDPSKTVRLSTSFNVRKEQF 124
           SYGFFQVINHGVR E+VE++IEVG +FF LP+EEKLK YSDDPSKTVRLSTSFNVRKEQF
Sbjct: 61  SYGFFQVINHGVRAEVVERMIEVGNEFFALPVEEKLKLYSDDPSKTVRLSTSFNVRKEQF 120

Query: 125 RNWRDYLRLHCYPLSNYTPHWPSNPPSFR 153
            NWRDYLRLHC+PL +Y PHWPSNPPSFR
Sbjct: 121 HNWRDYLRLHCHPLHHYIPHWPSNPPSFR 149

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLV02.9e-5057.05Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Q8W2X53.9e-4757.64Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... [more]
Q9ZSA82.9e-3451.08Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Q9ZSA73.2e-3348.94Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1[more]
Q6YYX91.8e-2337.18Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni... [more]
Match NameE-valueIdentityDescription
XP_004137205.18.05e-111100.00protein DOWNY MILDEW RESISTANCE 6 [Cucumis sativus] >KGN53620.1 hypothetical pro... [more]
XP_008462909.22.47e-9994.63PREDICTED: LOW QUALITY PROTEIN: protein DOWNY MILDEW RESISTANCE 6-like [Cucumis ... [more]
XP_038895989.16.62e-9387.92protein DOWNY MILDEW RESISTANCE 6 [Benincasa hispida][more]
XP_023552149.11.60e-8379.87protein DOWNY MILDEW RESISTANCE 6 [Cucurbita pepo subsp. pepo][more]
XP_022922602.14.56e-8379.87protein DOWNY MILDEW RESISTANCE 6 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KVJ33.90e-111100.001-aminocyclopropane-1-carboxylate oxidase OS=Cucumis sativus OX=3659 GN=Csa_4G09... [more]
A0A1S3CHY91.19e-9994.63LOW QUALITY PROTEIN: protein DOWNY MILDEW RESISTANCE 6-like OS=Cucumis melo OX=3... [more]
A0A6J1E3R82.21e-8379.87protein DOWNY MILDEW RESISTANCE 6 OS=Cucurbita moschata OX=3662 GN=LOC111430560 ... [more]
A0A6J1JAA43.60e-8279.87protein DOWNY MILDEW RESISTANCE 6 OS=Cucurbita maxima OX=3661 GN=LOC111482687 PE... [more]
A0A6J1CGV12.03e-7975.84protein DOWNY MILDEW RESISTANCE 6 OS=Momordica charantia OX=3673 GN=LOC111011401... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 194..292
e-value: 2.7E-29
score: 101.6
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 42..146
e-value: 1.2E-25
score: 90.5
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..330
e-value: 5.4E-102
score: 343.5
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 5..338
NoneNo IPR availablePANTHERPTHR47990:SF70PROTEIN DOWNY MILDEW RESISTANCE 6coord: 5..338
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 16..329
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 192..292
score: 13.510912

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G11887.T1Cucsat.G11887.T1mRNA
Cucsat.G11887.T2Cucsat.G11887.T2mRNA
Cucsat.G11887.T3Cucsat.G11887.T3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009805 coumarin biosynthetic process
biological_process GO:0002238 response to molecule of fungal origin
molecular_function GO:0016706 2-oxoglutarate-dependent dioxygenase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0046872 metal ion binding