Cucsat.G11753 (gene) Cucumber (B10) v3

Overview
NameCucsat.G11753
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionTranscription initiation factor IIE subunit beta
Locationctg1820: 4577394 .. 4580451 (+)
RNA-Seq ExpressionCucsat.G11753
SyntenyCucsat.G11753
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTAGATCTTAACTGCAATATTTGAAAATATAGGGGTCAAAGTATAAACATTCTAAAACTGAGGTACCAAAATGGAAGTTGAGTTCTTTCCAAGAACCTAACCCCATTTCTGATTTTAACCTTCCTCAAACGCACTCGTTCCTCAGCTTTCTCCCTCGCGCTTTTCCAATTCATTCTTCTCGATCTCCATACCTCCACAAACCCAACTCCATACAACAATCACCTCCATTACGTACACTACAGATCATTCGACATCGACCACCCATCTCACCGTCGTCTTATTCACGGCGGTTGATTTCGGTCTATCTAACCGATGCAATTTCTGGTATTTTACTACATACCCTTTTCTTTTCTGGGTATTTTCAATTGTGTGTTGGGCTTGATGGGACGCCTGGCAATTTTATTTTATTAGTTGAATCTATTTTGATGATCTTTGTCGTTATTTTGATTGTTAGGGTTGAGAATTATTGAAACTCTTAAGGGGATTTAATGGCGTTGCAAGAACAATTAGATAGGTTTAAGAAACAGCAAGAACGATGTCAGAGTACCTTGTCTGGCATTGCTGCATCCAAGTCTGTATCCAAGTCTACACCCAAGTTTACGCCTGCACCTGCTGCTTCCATTGCAAGACCTCCGGCTCCTGCTGTAAAATTTTCAAATGACACTGAGAGACTTCAACATATTAACAGCATAAGGAAGTCTCCAGTCGGGGCTCAGATCAAGAGGGTTATTGACCTGCTGCTGGAGGTTCATATCCATCACTCCATCTTTCTTATATTTCCCCGTTTGTTTTTCTCCTTCTATCTAATCTGCTGTACTTGGTTTCTTGTGTTTGACCGGATTCTTCAATGTAAGGCTTTGTATTGGATAGGATTTAATGGACCGTGTATTGATTTGAGCCGTGTAGACCTTATGCTGCTGAAATTTTGCATTTTATTCTGTCCAAGTTCCATAAGTTGAATGTGTGTTTTTTAATGGATACCGTTTTAACTGGGGGATTTTGCTGGAATGAGGACTTCTTCAGGTAGTGAGGCTTGTGATTGGCATTAGATGGCAAGAGGAGCCAGGACCCTCTTGAGTGGCTTTTGGGTGGTTGTTTTGCCTACATGTGTTGGTGCAAGATTGTATTTTTACATCCGAATTACATATGTTAATCTAATCCCTTCCCTGTAATCTTCTCTGGATTTTCAATTGGCTGTAAAAAAGCTAAAAAGAAGGCCTACTGAAATGCATTTTCTACTTACTGTTAGTGAATTGGAACCTTAGGGATATGTTACAAGCTTACCGCTTGCTTATTGTGCATGTTAACAGACAAGGCAAGCGTTTACACCTGAGCAAATCAATGAAGCATGTTATGTTGATATAAATTCCAACAAGGCTGTCTATGATAGTCTGCGGAATAACCCAAAGGTGCACTATGATGGGAGGCGTTTCTCATACAAGGTAATTTATATTCTCAAATACCTTTATTATTTACATCCTTGTACTTATTTGGTTTAAAATTTTGAACCTTAAGGTTGACATTTTTCCACTCCTTTTTTTCCCTTCCGTTTTTGGTGTTTTCAGTCCAAGCATGACCTGAAGGATAAGAATCAACTTCTTTACTTGGTACGAAAATTTCCAGAAGGCATTGCTGTCATTGATCTCAAGGATGCTTATCCAAGCGTTATGGAGGACTTGCAAGTATGTATATATATATATATATATATATATATATATTTAATTTTTTTTTCCAATTTTGAATGCAAAAACGTGAAAAATAACAAATGACTCTTGAATGATGCTATCATTAATATAACTTATTCAGCAAAATAACCTTTGTAAAGTTTAATGTATTGGCTTTTTGGACATCAAATGTCTATATGGCTTGCTCACTATTTATGTCGATGTTCAGGGTTCTTGTTCATGCTATGCCTCAATAAGTCTATTCTATTTTCTTCCTGGTTAGACTTTGGTTTCTGTTATAGACTAGAAGGTTTTGTTCATCCAACAATTCCTATCCCAAAATAGCAGCTGAAATTTGAATTGAAGGATTTAACTCATGATTTATAGTCTTGTCAAAACAAATACAAATTGTTTTTCTAATTCAGAGGAGGAAACAAAAAGAAAATTCAACAACAGGAGCCAATCTGAGACTAATGAGACGTAATGTCTCAATCTAAAACTTTAAGGTCTGACAAGTATTTACAATTCTTTGAAAAGAATATACTCAAGGAAGTATTGAACTTGGGGTTGTTGCATCCAAATTGTAGTTGATCACCGGCCCTCAAAATTATTACAACTTCTTTGCTTATATAGTGAATGGAAAATACACGGCTTTACTCTTTGTTTATGAATGGAAAACAGAGTCCGTTAACTTTAGTTTTTAGCTTACATTTTATTTTCCTCTCTTGTTTGTTTAGGCCCTAAAAGCCTCAGGCCAGGTTTGGCTTCTCTCAAACTTCGATTCACAAGAAGATATTGCCTACCCAAATGACCCACGAATTCAAATCAAGGTAGACGATGATCTGAAGCAGCTCTTTCGGGAGATCGAATTACCTCGTGACATGCTTGATGTCGAAAAGGATCTGCAGAAGAATGGAATGAAGCCTGCAACCAACACGGCAAAGAGGCGAGCCAACGCACAACACGTAGCATCCTCAAAACCCAAGCCGAAGAAGAAGAAGCAAGAAATCAGCAAGAGGACTAAGCTCACTAACGCACATCTACCCGAGCTCTTCAAAAACTAGAGAACCGTATAGACTGGCCTGCAACAATATAGTTTGTATGGATAATTCATGGACTCTGTAGATAGGATTCAATCATGCCTTCTGTTTGGTGTATTGCTATTTGTTTTCCATGATCTCATCATAGATTGAAATGCTCCTGTGATTTACTCTTCTTTGTTTACATCAATTAATTGAACAATTTGAAATGTAAGATATAAGTTCAACTAAACTAGATATATGGTATTAGAAGTTTTAGCATATATACCAACTTTTGAATTCTATTGTTTCAAATTGATACGTGTAACATCACTTCTATCCCCTGCTTTGCCGCAGACCTGTCCTTTGAAGGAGGTTATACTCTAATGACTAAAT

Coding sequence (CDS)

ATGGCGTTGCAAGAACAATTAGATAGGTTTAAGAAACAGCAAGAACGATGTCAGAGTACCTTGTCTGGCATTGCTGCATCCAAGTCTGTATCCAAGTCTACACCCAAGTTTACGCCTGCACCTGCTGCTTCCATTGCAAGACCTCCGGCTCCTGCTGTAAAATTTTCAAATGACACTGAGAGACTTCAACATATTAACAGCATAAGGAAGTCTCCAGTCGGGGCTCAGATCAAGAGGGTTATTGACCTGCTGCTGGAGACAAGGCAAGCGTTTACACCTGAGCAAATCAATGAAGCATGTTATGTTGATATAAATTCCAACAAGGCTGTCTATGATAGTCTGCGGAATAACCCAAAGGTGCACTATGATGGGAGGCGTTTCTCATACAAGTCCAAGCATGACCTGAAGGATAAGAATCAACTTCTTTACTTGGTACGAAAATTTCCAGAAGGCATTGCTGTCATTGATCTCAAGGATGCTTATCCAAGCGTTATGGAGGACTTGCAAGCCCTAAAAGCCTCAGGCCAGGTTTGGCTTCTCTCAAACTTCGATTCACAAGAAGATATTGCCTACCCAAATGACCCACGAATTCAAATCAAGGTAGACGATGATCTGAAGCAGCTCTTTCGGGAGATCGAATTACCTCGTGACATGCTTGATGTCGAAAAGGATCTGCAGAAGAATGGAATGAAGCCTGCAACCAACACGGCAAAGAGGCGAGCCAACGCACAACACGTAGCATCCTCAAAACCCAAGCCGAAGAAGAAGAAGCAAGAAATCAGCAAGAGGACTAAGCTCACTAACGCACATCTACCCGAGCTCTTCAAAAACTAG

Protein sequence

MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTERLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKVHYDGRRFSYKSKHDLKDKNQLLYLVRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLLSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRRANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN
Homology
[Close]
BLAST of Cucsat.G11753 vs. NCBI nr
Match: XP_011653191.1 (general transcription factor IIE subunit 2 [Cucumis sativus] >KGN53349.1 hypothetical protein Csa_014626 [Cucumis sativus])

HSP 1 Score: 159 bits (403), Expect = 2.63e-45
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE
Sbjct: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86
          RLQHINSIRKSPVGAQIKRVIDLLLE
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86

[Close]
BLAST of Cucsat.G11753 vs. NCBI nr
Match: XP_008452012.1 (PREDICTED: general transcription factor IIE subunit 2-like [Cucumis melo] >XP_008452013.1 PREDICTED: general transcription factor IIE subunit 2-like [Cucumis melo] >KAA0044840.1 general transcription factor IIE subunit 2-like [Cucumis melo var. makuwa] >TYK16624.1 general transcription factor IIE subunit 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 155 bits (393), Expect = 8.37e-44
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARP APAVKFSNDTE
Sbjct: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPLAPAVKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86
          RLQHINSIRKSPVGAQIKRVIDLLLE
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86

[Close]
BLAST of Cucsat.G11753 vs. NCBI nr
Match: XP_038906431.1 (general transcription factor IIE subunit 2-like [Benincasa hispida])

HSP 1 Score: 147 bits (372), Expect = 1.13e-40
Identity = 81/86 (94.19%), Postives = 83/86 (96.51%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQEQLDRFKKQQERCQ+TLSGIAA+KS SKSTPKFTPAPAAS  RPPAPAVKFSNDTE
Sbjct: 1  MALQEQLDRFKKQQERCQTTLSGIAAAKSASKSTPKFTPAPAAS--RPPAPAVKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86
          RLQHINSIRKSPVGAQIKRVIDLLLE
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 84

[Close]
BLAST of Cucsat.G11753 vs. NCBI nr
Match: XP_022136771.1 (general transcription factor IIE subunit 2-like [Momordica charantia])

HSP 1 Score: 142 bits (357), Expect = 2.01e-38
Identity = 77/92 (83.70%), Postives = 83/92 (90.22%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQ++LDRFKKQQERCQ+TL+GIAASKS   STPKFTPAPAA   RPPAPAVKFSNDTE
Sbjct: 1  MALQDKLDRFKKQQERCQTTLTGIAASKS--SSTPKFTPAPAAPNGRPPAPAVKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLEVHIHHS 92
          RLQHINSIRKSPVGAQIKRVIDLLLE  + H+
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLETRLAHT 90

[Close]
BLAST of Cucsat.G11753 vs. NCBI nr
Match: KAG6577183.1 (General transcription factor IIE subunit 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 134 bits (338), Expect = 4.01e-36
Identity = 74/86 (86.05%), Postives = 82/86 (95.35%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQE+LDRFK+QQERCQ+TLSGIAASK+ + +TP+FTPAPAAS  RPPAPA+KFSNDTE
Sbjct: 1  MALQEKLDRFKRQQERCQTTLSGIAASKTTT-TTPRFTPAPAAS--RPPAPAIKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86
          RLQHINSIRKSPVGAQIKRVIDLLLE
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 83

[Close]
BLAST of Cucsat.G11753 vs. ExPASy TrEMBL
Match: A0A0A0KWU5 (Transcription initiation factor IIE subunit beta OS=Cucumis sativus OX=3659 GN=Csa_4G049080 PE=3 SV=1)

HSP 1 Score: 159 bits (403), Expect = 1.27e-45
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE
Sbjct: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86
          RLQHINSIRKSPVGAQIKRVIDLLLE
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86

[Close]
BLAST of Cucsat.G11753 vs. ExPASy TrEMBL
Match: A0A1S3BS95 (Transcription initiation factor IIE subunit beta OS=Cucumis melo OX=3656 GN=LOC103493148 PE=3 SV=1)

HSP 1 Score: 155 bits (393), Expect = 4.05e-44
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARP APAVKFSNDTE
Sbjct: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPLAPAVKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86
          RLQHINSIRKSPVGAQIKRVIDLLLE
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86

[Close]
BLAST of Cucsat.G11753 vs. ExPASy TrEMBL
Match: A0A5A7TNR4 (Transcription initiation factor IIE subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004520 PE=3 SV=1)

HSP 1 Score: 155 bits (393), Expect = 4.05e-44
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARP APAVKFSNDTE
Sbjct: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPLAPAVKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86
          RLQHINSIRKSPVGAQIKRVIDLLLE
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86

[Close]
BLAST of Cucsat.G11753 vs. ExPASy TrEMBL
Match: A0A6J1C4F9 (Transcription initiation factor IIE subunit beta OS=Momordica charantia OX=3673 GN=LOC111008395 PE=4 SV=1)

HSP 1 Score: 142 bits (357), Expect = 9.73e-39
Identity = 77/92 (83.70%), Postives = 83/92 (90.22%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQ++LDRFKKQQERCQ+TL+GIAASKS   STPKFTPAPAA   RPPAPAVKFSNDTE
Sbjct: 1  MALQDKLDRFKKQQERCQTTLTGIAASKS--SSTPKFTPAPAAPNGRPPAPAVKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLEVHIHHS 92
          RLQHINSIRKSPVGAQIKRVIDLLLE  + H+
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLETRLAHT 90

[Close]
BLAST of Cucsat.G11753 vs. ExPASy TrEMBL
Match: A0A6J1J372 (Transcription initiation factor IIE subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111482983 PE=4 SV=1)

HSP 1 Score: 134 bits (338), Expect = 6.62e-36
Identity = 74/86 (86.05%), Postives = 82/86 (95.35%), Query Frame = 0 

Query: 1  MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60
          MALQE+LDRFK+QQERCQ+TLSGIAASK+ + +TP+FTPAPAAS  RPPAPA+KFSNDTE
Sbjct: 1  MALQEKLDRFKRQQERCQTTLSGIAASKTTT-TTPRFTPAPAAS--RPPAPAIKFSNDTE 60

Query: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 86
          RLQHINSIRKSPVGAQIKRVIDLLLE
Sbjct: 61 RLQHINSIRKSPVGAQIKRVIDLLLE 83

The following BLAST results are available for this feature:
BLAST of Cucsat.G11753 vs.
Match NameE-valueIdentityDescription
BLAST of Cucsat.G11753 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011653191.12.63e-45100.00general transcription factor IIE subunit 2 [Cucumis sativus] >KGN53349.1 hypothe... [more]
XP_008452012.18.37e-4498.84PREDICTED: general transcription factor IIE subunit 2-like [Cucumis melo] >XP_00... [more]
XP_038906431.11.13e-4094.19general transcription factor IIE subunit 2-like [Benincasa hispida][more]
XP_022136771.12.01e-3883.70general transcription factor IIE subunit 2-like [Momordica charantia][more]
KAG6577183.14.01e-3686.05General transcription factor IIE subunit 2, partial [Cucurbita argyrosperma subs... [more]
BLAST of Cucsat.G11753 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KWU51.27e-45100.00Transcription initiation factor IIE subunit beta OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3BS954.05e-4498.84Transcription initiation factor IIE subunit beta OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A5A7TNR44.05e-4498.84Transcription initiation factor IIE subunit beta OS=Cucumis melo var. makuwa OX=... [more]
A0A6J1C4F99.73e-3983.70Transcription initiation factor IIE subunit beta OS=Momordica charantia OX=3673 ... [more]
A0A6J1J3726.62e-3686.05Transcription initiation factor IIE subunit beta OS=Cucurbita maxima OX=3661 GN=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040501TFA2, Winged helix domain 2PFAMPF18121TFA2_Winged_2coord: 136..193
e-value: 4.6E-14
score: 51.8
IPR016656Transcription initiation factor TFIIE, beta subunitPIRSFPIRSF016398TFIIE-betacoord: 1..277
e-value: 8.8E-25
score: 85.4
IPR016656Transcription initiation factor TFIIE, beta subunitPANTHERPTHR12716TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNITcoord: 1..277
IPR003166Transcription factor TFIIE beta subunit, DNA-binding domainPFAMPF02186TFIIE_betacoord: 75..134
e-value: 2.4E-9
score: 37.4
IPR003166Transcription factor TFIIE beta subunit, DNA-binding domainPROSITEPS51351TFIIE_BETA_Ccoord: 61..136
score: 10.965059
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..277
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 229..243
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..52
NoneNo IPR availablePANTHERPTHR12716:SF13BNAA02G21830D PROTEINcoord: 1..277

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G11753.T2Cucsat.G11753.T2mRNA
Cucsat.G11753.T1Cucsat.G11753.T1mRNA
Cucsat.G11753.T3Cucsat.G11753.T3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0006413 translational initiation
cellular_component GO:0005673 transcription factor TFIIE complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003743 translation initiation factor activity