Cucsat.G1105 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G1105
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	tRNA (Guanine-N(7)-)-methyltransferase
Location	ctg1: 7942442 .. 7945008 (+)
RNA-Seq Expression	Cucsat.G1105
Synteny	Cucsat.G1105

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGAAGATGATAGAGAGTGAATTAACCGAAACAGCTCCACTTCGAGGAGAGGCAAAATGGCTTCCTCCCTTTGCTTGCATTTACAAGCTTCCTCCCACCTCCGCTGCATTGCGGCTGCGAAGCCGGCGGCGCCTTCGATGGTCGCAACTGCGAACAAAATCTTATCTAAACGACCATTCCTTCGAAACTTCTCTTTACCATCGGGCTCTTCGGCCGACCTTTGCGAGCGGCAACCTCTCCGGAGTTCGGAATTAGTGGCGTTGGAATATGCCGACCTCAATCTCTCCCACAAAATGGTCGAGGTTTGTGTCTCTGTTCTTTAATTTTTGCCCTTATTTCTGATATCTTTGGCGAGTAATTGAAAACTGAGTGTGCTGATTATAATTAATGTGCATTTTTCTTGGTTTACTTATAATTGCAGGATTTGGGCCACGTTAGAATCAGACAGCATGTTAATCCTCTTAGTTCTTCTTTTTCTGTATGTAATTTGATATTTGAAACTGGGTTTGGCTTCTCTCTGATGAAGATGAACAATTAAGAACTGTGGGATTGGAATTTGAGCTTAGTGTTTGCTTAATTGAAGGAGTGCTTTCCCTCTTTTACCTTTCAGGTGCCCACACCAGCACCAAACTGGGATGAAGTCTTCAGGGATCCCACGTTGCCCCTCATGGTCGATATAGGAAGTGGTGAGTAAGTTGATGCATTCTCTGTTATATACTTATAACTATCTATTTTCGTCTTAATTCCAACTTTTTTCTTTGTATCCAAGCTACTTATGCATACTATGATTTCATGGGATAATATTTGATTTTTCTATACCATGTGCTTGAATCCATGTCCGTAATTTTGAAATGGTTAAAATCTTGTTCGTCCCTATAAAATATGCTTCATATGCATTTATTCATTCATAATCGATTATGATGACATGAAAATAGTGTTTAAAAGTGTAAAATTTAACAATAGATTGATTTTAAATGATTAAATAGTGATTTTGAACATAAAGAGATCACTCTTAAGTATGTCATTATTATAGTCCGTCTCTTACCCATTATCCCAGTTTGGGGTAAGCAGAGGATAGTCATGAAGAAATCTTTGAAACTAATGCATCCACCGTGATTTAAACCTTGGGCTAGCTAGTAAAAGTTAATACGATATCAGTCACCTAGGTTGACCTGAATAATTTACAATGGATTTGCTGTGGTCTACATTGAAAATGTTAGTACACTTTATGAAAATCTTGATTACCTCTCAGTTTTGGTTTAACTACCCTTGTTACATTCTTTTTGTGGGATTCACGAGGCCGTTTTATATATTGTTTACCCCAGCAATATTCTAAGTATTCCTATTCCCAGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAGACACCCTGAGTCAAGAAATTTCTTGGGTCTTGAAATACGACAAAAAGTACGGGACCAAAGATATTCATTCCTTTAGAGTCTTAATTGGTTTGAACTTTTTGATCCTCATTCGCTTTCTTTTGTGCTCTTGCAGCTGGTCAAGCGTGCTGGTATTTGGGTTAAAGAGTTGGGGTTTAGCAACGTGTAAGGACCTTAACCTCATATCAGGTAGCGAAAAGCAAGATCATCTTTTTGCTATTGGTACATTAGTAATGAACTTTCTGAACTTGAATGCAGACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATTATATCTTCATATCCTGGGCCCTTGATACTGGTTTCCATACTGGTTAGTGCACAACCATTCATAACACACTATACTGCCATATTTTTCAGTCCCCCCACCCAAACAAATCAACCCCAACTTCCCCATTGATCCAGTCCGATAAATATCGACAAAGATAGAACTCACCCATGTTTAATCTGATTGCAGTGCCCAGATCCCCATTTCAAGAAACGACATCATAAACGAAGGGTTTTGCAGAAGCCTTTGGTAGAATCTATAGTAGATAGTTTAATGCCAGGAGGACAGGTATATATGATCTTCTGATTATCCATCCCTGACAATGAATGATCTGTTGCATTGTCTGATGTTGTGAATTTTAATCTATACAATTCAACAGGTGTTCATACAGTCCGACGTGCTCGAAGTGGCAGCAGACATGAGACTTTGGTTTGATTCTGAAGCCCATATTCTGGAACACATTAACAAGATCGACAAGAGTGTTTTGTGTGATAAAGATGGATGGTTGTTGAACAATCCAATGGGAATAAGGACAGAAAGAGAAATTCACGCTGAATCAGAAGGTGCAAACATTTATAGAAGAATGTACTCAAAAGTACACTTGACTAAAGAAGAATAACTAACTTACTTACTAGATGGATTGTTTAATGATATAATCTTAATCTATTTACATTTACATTTGAGTCATGCTTTCTGCCTAATATTGGTATTGGGAAGTTGAAAAAAAAATTTCCCATGAAATTAGGAAAGAAATAGAAATTAGGATTAGGGTTGGAAAATATAACGAAAAATACGGTGGGTCCCACCTGCCTCTATCTTGTTTCCACCTGGCGCCAACATAGATGACTAGTCAAATTAAAT

Coding sequence (CDS)

ATGGCTTCCTCCCTTTGCTTGCATTTACAAGCTTCCTCCCACCTCCGCTGCATTGCGGCTGCGAAGCCGGCGGCGCCTTCGATGGTCGCAACTGCGAACAAAATCTTATCTAAACGACCATTCCTTCGAAACTTCTCTTTACCATCGGGCTCTTCGGCCGACCTTTGCGAGCGGCAACCTCTCCGGAGTTCGGAATTAGTGGCGTTGGAATATGCCGACCTCAATCTCTCCCACAAAATGGTCGAGGATTTGGGCCACGTTAGAATCAGACAGCATGTTAATCCTCTTAGTTCTTCTTTTTCTGTGCCCACACCAGCACCAAACTGGGATGAAGTCTTCAGGGATCCCACGTTGCCCCTCATGGTCGATATAGGAAGTGGTAGTGGCAGATTTCTCTTGTGGCTTGCAAAAAGACACCCTGAGTCAAGAAATTTCTTGGGTCTTGAAATACGACAAAAACTGGTCAAGCGTGCTGGTATTTGGGTTAAAGAGTTGGGGTTTAGCAACGTGTAA

Protein sequence

MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV

Homology

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BLAST of Cucsat.G1105 vs. ExPASy Swiss-Prot
Match: Q2JRH1 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=trmB PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 8.5e-21
Identity = 48/83 (57.83%), Postives = 60/83 (72.29%), Query Frame = 0


Query: 88  RIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPLMVDIGSGSGRFLLWLAKRHPESRNFLG 147
           R+RQHVNPL + +  PTP P+W+ V+R    PL +DIGSGSGRFLL +A+  P+  NFLG
Sbjct: 34  RLRQHVNPLQAQYQQPTPPPHWERVYRRLGQPLHLDIGSGSGRFLLRMAQEQPD-WNFLG 93

Query: 148 LEIRQKLVKRAGIWVKELGFSNV 171
           +EIRQ LV+RA  W  ELG  NV
Sbjct: 94  VEIRQPLVERANAWRDELGLDNV 115

[Close]

BLAST of Cucsat.G1105 vs. ExPASy Swiss-Prot
Match: Q2JJQ0 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=trmB PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.2e-20
Identity = 48/87 (55.17%), Postives = 61/87 (70.11%), Query Frame = 0


Query: 84  LGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPLMVDIGSGSGRFLLWLAKRHPESR 143
           LGH R+RQHVNPL + +  P P P+W+ V+R    P  +DIG+GSGRFLL +A+  P+  
Sbjct: 34  LGH-RVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRFLLRIAQEQPD-W 93

Query: 144 NFLGLEIRQKLVKRAGIWVKELGFSNV 171
           NFLG+EIRQ LV+RA  W  ELG  NV
Sbjct: 94  NFLGVEIRQALVERANAWRDELGLDNV 118

[Close]

BLAST of Cucsat.G1105 vs. ExPASy Swiss-Prot
Match: B0JUK5 (tRNA (guanine-N(7)-)-methyltransferase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=trmB PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.7e-16
Identity = 43/87 (49.43%), Postives = 58/87 (66.67%), Query Frame = 0


Query: 84  LGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPLMVDIGSGSGRFLLWLAKRHPESR 143
           +  VR+RQHVNPLS  +  P   P+W++V++D T PL +DIG   G+FLL +A+  PE  
Sbjct: 1   MAKVRVRQHVNPLSHKYRHPIAPPDWNQVYQDMTQPLHLDIGCARGKFLLQMAQVEPEI- 60

Query: 144 NFLGLEIRQKLVKRAGIWVKELGFSNV 171
           NFLG+EIRQ LV  A    + LG SN+
Sbjct: 61  NFLGIEIRQPLVIEANQERERLGLSNL 86

[Close]

BLAST of Cucsat.G1105 vs. ExPASy Swiss-Prot
Match: P72546 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=trmB PE=3 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 2.4e-15
Identity = 42/87 (48.28%), Postives = 54/87 (62.07%), Query Frame = 0


Query: 84  LGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPLMVDIGSGSGRFLLWLAKRHPESR 143
           +  VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A   PE  
Sbjct: 1   MARVRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQPE-W 60

Query: 144 NFLGLEIRQKLVKRAGIWVKELGFSNV 171
           N+LGLEIR+ LV  A    +E   +N+
Sbjct: 61  NYLGLEIREPLVDEANAIARERELTNL 86

[Close]

BLAST of Cucsat.G1105 vs. ExPASy Swiss-Prot
Match: Q5N2X5 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=trmB PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.4e-15
Identity = 42/87 (48.28%), Postives = 54/87 (62.07%), Query Frame = 0


Query: 84  LGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPLMVDIGSGSGRFLLWLAKRHPESR 143
           +  VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A   PE  
Sbjct: 1   MARVRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQPE-W 60

Query: 144 NFLGLEIRQKLVKRAGIWVKELGFSNV 171
           N+LGLEIR+ LV  A    +E   +N+
Sbjct: 61  NYLGLEIREPLVDEANAIARERELTNL 86

[Close]

BLAST of Cucsat.G1105 vs. NCBI nr
Match: XP_004133932.1 (uncharacterized protein LOC101218266 [Cucumis sativus] >KGN56621.1 hypothetical protein Csa_011077 [Cucumis sativus])

HSP 1 Score: 338 bits (866), Expect = 8.58e-115
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP 60
           MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP
Sbjct: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP 60

Query: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120
           LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL
Sbjct: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120

Query: 121 MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV
Sbjct: 121 MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170

[Close]

BLAST of Cucsat.G1105 vs. NCBI nr
Match: KAA0049058.1 (tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo var. makuwa] >TYK17505.1 tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 325 bits (833), Expect = 9.92e-110
Identity = 163/170 (95.88%), Postives = 165/170 (97.06%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP 60
           MASSLCLHLQ  SHLRC+ AAKPAAPS+VATANKILSKRPFLRNFSLPSGSSADLCERQP
Sbjct: 1   MASSLCLHLQTCSHLRCLGAAKPAAPSVVATANKILSKRPFLRNFSLPSGSSADLCERQP 60

Query: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120
           LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL
Sbjct: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120

Query: 121 MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           MVDIGSGSGRFLLWLAKRH ESRNFLGLEIRQKLVKRAG WVKELGFSNV
Sbjct: 121 MVDIGSGSGRFLLWLAKRHLESRNFLGLEIRQKLVKRAGFWVKELGFSNV 170

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BLAST of Cucsat.G1105 vs. NCBI nr
Match: XP_008438199.1 (PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo])

HSP 1 Score: 324 bits (831), Expect = 2.00e-109
Identity = 163/170 (95.88%), Postives = 165/170 (97.06%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP 60
           MASSLCLHLQ  SHLRC+AAAKPAAPS+VATANKILSKRPFLRNFSLPSGSSADLCERQP
Sbjct: 1   MASSLCLHLQTCSHLRCLAAAKPAAPSVVATANKILSKRPFLRNFSLPSGSSADLCERQP 60

Query: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120
           LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL
Sbjct: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120

Query: 121 MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           MVDIGSGSGRFLLWLAK H ESRNFLGLEIRQKLVKRAG WVKELGFSNV
Sbjct: 121 MVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNV 170

[Close]

BLAST of Cucsat.G1105 vs. NCBI nr
Match: XP_038879912.1 (tRNA (guanine-N(7)-)-methyltransferase [Benincasa hispida])

HSP 1 Score: 293 bits (749), Expect = 4.91e-97
Identity = 152/173 (87.86%), Postives = 156/173 (90.17%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILS---KRPFLRNFSLPSGSSADLCE 60
           MASSLCLHLQ  SHLR IAAAK AAPS VATANK LS   KRPFL NF LPSGSSADLCE
Sbjct: 1   MASSLCLHLQTCSHLRYIAAAKAAAPSRVATANKTLSLFLKRPFLPNFCLPSGSSADLCE 60

Query: 61  RQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPT 120
           RQPLRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+DPT
Sbjct: 61  RQPLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPT 120

Query: 121 LPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           LPLMVDIG GSGRFLLWLAKRH +SRNFLGLEIR+KLVKRA IWVKELGFSNV
Sbjct: 121 LPLMVDIGCGSGRFLLWLAKRHFDSRNFLGLEIREKLVKRADIWVKELGFSNV 173

[Close]

BLAST of Cucsat.G1105 vs. NCBI nr
Match: XP_022924717.1 (uncharacterized protein LOC111432133 [Cucurbita moschata])

HSP 1 Score: 276 bits (706), Expect = 1.95e-90
Identity = 144/176 (81.82%), Postives = 154/176 (87.50%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAA---PSMVATANKILS---KRPFLRNFSLPSGSSAD 60
           MASSLC  L   S LRCIAAAK AA   PS VATAN+ LS   KRPFL++F LPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 120
           LCERQ LRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           DPTLPLMVDIGSGSGRFLLWLAK+H +SRNFLGLEIRQKLVKRA +WVKELGF+NV
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNV 176

[Close]

BLAST of Cucsat.G1105 vs. ExPASy TrEMBL
Match: A0A0A0L947 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126850 PE=4 SV=1)

HSP 1 Score: 338 bits (866), Expect = 4.16e-115
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP 60
           MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP
Sbjct: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP 60

Query: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120
           LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL
Sbjct: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120

Query: 121 MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV
Sbjct: 121 MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170

[Close]

BLAST of Cucsat.G1105 vs. ExPASy TrEMBL
Match: A0A5A7U1F8 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003710 PE=4 SV=1)

HSP 1 Score: 325 bits (833), Expect = 4.80e-110
Identity = 163/170 (95.88%), Postives = 165/170 (97.06%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP 60
           MASSLCLHLQ  SHLRC+ AAKPAAPS+VATANKILSKRPFLRNFSLPSGSSADLCERQP
Sbjct: 1   MASSLCLHLQTCSHLRCLGAAKPAAPSVVATANKILSKRPFLRNFSLPSGSSADLCERQP 60

Query: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120
           LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL
Sbjct: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120

Query: 121 MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           MVDIGSGSGRFLLWLAKRH ESRNFLGLEIRQKLVKRAG WVKELGFSNV
Sbjct: 121 MVDIGSGSGRFLLWLAKRHLESRNFLGLEIRQKLVKRAGFWVKELGFSNV 170

[Close]

BLAST of Cucsat.G1105 vs. ExPASy TrEMBL
Match: A0A1S3AVT2 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 PE=4 SV=1)

HSP 1 Score: 324 bits (831), Expect = 9.67e-110
Identity = 163/170 (95.88%), Postives = 165/170 (97.06%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAAPSMVATANKILSKRPFLRNFSLPSGSSADLCERQP 60
           MASSLCLHLQ  SHLRC+AAAKPAAPS+VATANKILSKRPFLRNFSLPSGSSADLCERQP
Sbjct: 1   MASSLCLHLQTCSHLRCLAAAKPAAPSVVATANKILSKRPFLRNFSLPSGSSADLCERQP 60

Query: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120
           LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL
Sbjct: 61  LRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRDPTLPL 120

Query: 121 MVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           MVDIGSGSGRFLLWLAK H ESRNFLGLEIRQKLVKRAG WVKELGFSNV
Sbjct: 121 MVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNV 170

[Close]

BLAST of Cucsat.G1105 vs. ExPASy TrEMBL
Match: A0A6J1E9S1 (uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC111432133 PE=4 SV=1)

HSP 1 Score: 276 bits (706), Expect = 9.45e-91
Identity = 144/176 (81.82%), Postives = 154/176 (87.50%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAA---PSMVATANKILS---KRPFLRNFSLPSGSSAD 60
           MASSLC  L   S LRCIAAAK AA   PS VATAN+ LS   KRPFL++F LPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 120
           LCERQ LRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           DPTLPLMVDIGSGSGRFLLWLAK+H +SRNFLGLEIRQKLVKRA +WVKELGF+NV
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNV 176

[Close]

BLAST of Cucsat.G1105 vs. ExPASy TrEMBL
Match: A0A6J1ITE1 (uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752 PE=4 SV=1)

HSP 1 Score: 276 bits (705), Expect = 1.34e-90
Identity = 144/176 (81.82%), Postives = 153/176 (86.93%), Query Frame = 0


Query: 1   MASSLCLHLQASSHLRCIAAAKPAA---PSMVATANKILS---KRPFLRNFSLPSGSSAD 60
           MASSLC  L   S LRCIAAAK AA   PS VATAN+ LS   KRPFL +F LPSGSSA+
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLHSFCLPSGSSAE 60

Query: 61  LCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 120
           LCERQ LRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 170
           DPTLPLMVDIGSGSGRFLLWLAK+H +SRNFLGLEIRQKLVKRA +WVKELGF+NV
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNV 176

[Close]

BLAST of Cucsat.G1105 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 183.0 bits (463), Expect = 2.1e-46
Identity = 100/176 (56.82%), Postives = 124/176 (70.45%), Query Frame = 0


Query: 3   SSLCLHLQASSHLRCIAAAK----PAAPSMVATANKILSKRPFLRNF---SLPSGSSADL 62
           +SLC    +SS    + A +     ++P +    +KI       R F   S  S +S  +
Sbjct: 2   ASLCQACSSSSATPLLLAKRVFSLASSPFIFMREHKIPRHCNSFRAFNAVSASSSASTSV 61

Query: 63  C-ERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 122
           C + + LRS+ELV LEYA+LNL+HK+ E++GHVRIRQHVNPLSSSFS P P P WDEV++
Sbjct: 62  CVDFKDLRSNELVELEYAELNLNHKISEEVGHVRIRQHVNPLSSSFSKPAPVPVWDEVYK 121

Query: 123 DPTLPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNV 171
           DP+LPLMVDIGSGSGRFLLW A ++ ESRN+LGLEIRQKLVKRA  WV ELG SNV
Sbjct: 122 DPSLPLMVDIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGLSNV 177

The following BLAST results are available for this feature:

BLAST of Cucsat.G1105 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2JRH1	8.5e-21	57.83	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX...	[more]
Q2JJQ0	3.2e-20	55.17	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-...	[more]
B0JUK5	1.7e-16	49.43	tRNA (guanine-N(7)-)-methyltransferase OS=Microcystis aeruginosa (strain NIES-84...	[more]
P72546	2.4e-15	48.28	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 79...	[more]
Q5N2X5	2.4e-15	48.28	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 /...	[more]

BLAST of Cucsat.G1105 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004133932.1	8.58e-115	100.00	uncharacterized protein LOC101218266 [Cucumis sativus] >KGN56621.1 hypothetical ...	[more]
KAA0049058.1	9.92e-110	95.88	tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo var. makuwa] >TYK17505.1 tR...	[more]
XP_008438199.1	2.00e-109	95.88	PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo]	[more]
XP_038879912.1	4.91e-97	87.86	tRNA (guanine-N(7)-)-methyltransferase [Benincasa hispida]	[more]
XP_022924717.1	1.95e-90	81.82	uncharacterized protein LOC111432133 [Cucurbita moschata]	[more]

BLAST of Cucsat.G1105 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L947	4.16e-115	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126850 PE=4 SV=1	[more]
A0A5A7U1F8	4.80e-110	95.88	tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3AVT2	9.67e-110	95.88	tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 P...	[more]
A0A6J1E9S1	9.45e-91	81.82	uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC1114321...	[more]
A0A6J1ITE1	1.34e-90	81.82	uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752...	[more]

BLAST of Cucsat.G1105 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17660.1	2.1e-46	56.82	tRNA (guanine-N-7) methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 2..213 e-value: 2.8E-55 score: 188.7
None	No IPR available	PANTHER	PTHR23417:SF21	TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE	coord: 5..208
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 26..139 e-value: 9.70845E-5 score: 38.5651
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	TIGRFAM	TIGR00091	TIGR00091	coord: 11..207 e-value: 1.8E-34 score: 116.7
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PFAM	PF02390	Methyltransf_4	coord: 23..202 e-value: 2.6E-35 score: 121.4
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PANTHER	PTHR23417	3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE	coord: 5..208
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PROSITE	PS51625	SAM_MT_TRMB	coord: 1..207 score: 51.97023
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 24..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G1105.T3	Cucsat.G1105.T3	mRNA
Cucsat.G1105.T4	Cucsat.G1105.T4	mRNA
Cucsat.G1105.T5	Cucsat.G1105.T5	mRNA
Cucsat.G1105.T6	Cucsat.G1105.T6	mRNA
Cucsat.G1105.T2	Cucsat.G1105.T2	mRNA
Cucsat.G1105.T13	Cucsat.G1105.T13	mRNA
Cucsat.G1105.T7	Cucsat.G1105.T7	mRNA
Cucsat.G1105.T10	Cucsat.G1105.T10	mRNA
Cucsat.G1105.T8	Cucsat.G1105.T8	mRNA
Cucsat.G1105.T11	Cucsat.G1105.T11	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106004	tRNA (guanine-N7)-methylation
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0043527	tRNA methyltransferase complex
molecular_function	GO:0008176	tRNA (guanine-N7-)-methyltransferase activity