Cucsat.G10613 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G10613
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	DNA-directed RNA polymerases II and IV subunit 5A-like
Location	ctg1679: 219750 .. 222326 (+)
RNA-Seq Expression	Cucsat.G10613
Synteny	Cucsat.G10613

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAAACGAACCCTCCATGCAATTTTTCCTCCCCTTCTTCACGTTAACTCTTCGCTTTCTCTCTCTTCCATTCATCTTTCTCCTTCTTCCTCCGGCGACCTGTTCGACGGCGTCCGGGGCAATCTCCTCCAACGACAGTGTGGTTCTTCCCTCTCGACAGCACGTACGTGAGCAAGGCGTACGAGCGAGCCGACGGCATTGACGCGAAAACCTCCTTATCGAGCCCAGGGATCCCACGAACCAGCAACGATCACGCACAGCAGCAGCGTCGAACGTGACTCAGGCGGTTTGCAAACCCTAAAACTGCGTTTTCCCCCATCCTAAAAGGTTTGAAACTTACCCATTTTTGTTCGGCATTATATTTGAAATACCCACTTCTGTGTTGAATTTCTGTGATTTGATGATTCCCTTTTGTTTGGCTTTGATATTCTTATATGCTTTAGGATTAGTCGAGTTGAGGGGAATTTCTATAGATTTTATCTTTCGTTTTCGTTTTTTCCCCCTTTCCCCCCTGTTATTTGGTTTAGGAATTGAGTTGTATACGCTTTTATGTGCATCTTATTGAGAATAATGATTGAATACTGAATTTCTTGAAAATGTGTTTAGGCTGAAAGAGATGGTGTTCCCAGAAGAAGAAATCACCAAACTCTTTAGAGTCCGAAAAACTGTGTTGCAGATGTTGAAAGACAGAGGCTATTTTGTTGGGGATTTCGAGCTTGATATGTCTAAAGTACAATTTAAGAATAAGTTTGGTGAGAACATGAAAAGAGAAGATCTGGTTATCAATAAATCCAAGAGAAATAATAGCTCTGATCAGGTATAGTTTCATACTACACAATGATAATCTCATTTATGTATGACTCTCTTATAACTATTTAGTTTTCAATTTTTAGTTTTTCAAGATTGTGTTTGATGCTCCCAACTCATTGACTATGATTTTCATTTTCTAATGTAAACATTGAATTCGGAGCAAGTTTCAATAGAAAAATGTTTTTAAAAATTACTATTCTTTAGTTTTCAAAACTTGGCTTAAATTTTGAAGACTCACATAAAAAGAAGAATATAACAAAACATAGAAATCAATAGGTTTAAGTAATATTTACAAGCTTAATTTTCAAGAACCAAAAGCTTAGGGTGTGTTTGGCCCAAGGAGTTGTGAAATATAAGGTAAGAGCTTAGAAGTAGTAGTTGTGAACTTCACTCCTTGTTTGGCTCGAGGAGTTGGTGGGTCCTACTCCTAAATAACTTCAATTTTATATGGTGGACCTCAAAGTTCACAACTCTCTGAGTTTCATTACTCCTTAGTCCTCAATATTTCACTCCGTATCCCAAACACTTGTCTGGCTCACCAACTTCTAAGTTAGTGTTAAATATTTCAACCTCAATGGTTTTCACTACCAAAGTTGCACATTTATTAAGGAACTCCGTCTTGCAATTCTGTAACCCCAAACACATGGTTAATAACTATAACATATTAACTCCAAACTTCCTCACTCAACTTAGCACCCCAACACCCCTCAATTGTTATAAAACAGGGTCTCATTGACTCATGAAATTTATTGTGTATTTAAACATGTTTAAACTTTACCTTAATAAAGGGTTGCAGTAAGTTTCCTCGGAAGAAGTTTCTATTTAAGTCATTCTCAAGTACTGTTACTGTTTGACATGGAAAATAGTTAACTATCGAAAAATATTTACCACATTTATATAAAGCTTGGTTATAGGGATGCACAGGTTATTTGTTAACCCTTTTATTTGTTTCCTTCTTTTGATTTATGACTCTTTTACACAGATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTGGTATAAAGATAGTGGAAACTTATGTAGGTCGTATGAAATCAGAGAATGTTTTCAGAGCAATATTTGTTGTTCAACTGAATTTGACCCCCTTTGCCCGAAATAAAATCGCCGAATTATCGTCTTCCAAACTCCATTTGGAGGTTTTCCAGGTAAAAGTAAAATCTCTTATAGTTTTTGGAGCCTTTTAATGTTGAATAGATTATTTTAATATCATCACATGCTACAAATTTAACGAGAACTAGAGAGGATTTTCTATTTTCCTATCCCAAAACCAGTTCTCTCGTACTTTTGTTGTCTCTCACAACAACAATGATGGGGGGTGGGGGAAGAATGGTGACGTTAGTTTGTAGTTACTTATGTGATTTTCATTTTCCATATAATAATGTCTTGCCTACCTTCTTATTTTGCTGACGTTAACGTTTACCTCTTTGTCATTTTTTTATGCAGGAAACAGAATTGTTGGTTAACATCAAAGAGCATGTTCTCATTCCCGAGCACCAGACACTCACAGACGAAGAGAAGAAAACGTTGTTGGGGAGGTATACGGTGAAAGAAACACAAGTGAGTTTCATTTTATTTGATCTTATTTTCTTGAAATACAAGAATATTGTGAGAATTGTGTTCCATAAAATATGTTGAAATAACTTTGGTAGCTTGGCCCGTGTTGAATAAGATTTTTGTAAATGATGAAGATATTAGGAGAGTTCAAGTCATGATGATTGCCTACCTAAAATTTAATTTTCTACACGTTACTCTGACAATCAAATATAGTAACGTTGGATGGTTGTTTTGTTAGAATAGTTAAGATGCGTTCAAGTTGGCTCTAACAGTGTTGGATACATATTGAAAATTGGTATACGTTAGTTTTTAACAAACATTTTGCATTTTGTCTCAGCTACCTCGCATGCAAGTTACCGATCCAATTTCTAGATATTATGGGCTGAAGCGTGGACATGTAGTGAAGATCATCCGACCAAGCGAAACTGCTGGAAGATATGTGACTTATAGATATGTTGTTTGAGATCAATCTGATATGAGTAATTGAAGTCATGATATTTCTGGAGTGGAAGTGGTTTGGAGTTTTAGGCAATTCTCCTTTATGAGCTTCAACTGGGGAATTCGTGCATTTAGAATTTAAATTGAACATCCACTCACGGTTTTTTTTTATTCTTTCATTGACGTAAGCATGTTTTTTGTAAAGGAAGGGAAAAAACGCTTACTGGTACACCAAATATTGAGGTTTTTGGTGACAAAGAAAGTCGATTAACAAGTTTATCCTTTTGGATCTTTTTGGTTGATCTTTCATTGCTAAAACATTTTCTTATTGTGAGGTTGATGAAAA

Coding sequence (CDS)

ATGGTGTTCCCAGAAGAAGAAATCACCAAACTCTTTAGAGTCCGAAAAACTGTGTTGCAGATGTTGAAAGACAGAGGCTATTTTGTTGGGGATTTCGAGCTTGATATGTCTAAAGTACAATTTAAGAATAAGTTTGGTGAGAACATGAAAAGAGAAGATCTGGTTATCAATAAATCCAAGAGAAATAATAGCTCTGATCAGATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTGGTATAAAGATAGTGGAAACTTATGTAGGTCGTATGAAATCAGAGAATGTTTTCAGAGCAATATTTGTTGTTCAACTGAATTTGACCCCCTTTGCCCGAAATAAAATCGCCGAATTATCGTCTTCCAAACTCCATTTGGAGGTTTTCCAGGAAACAGAATTGTTGGTTAACATCAAAGAGCATGTTCTCATTCCCGAGCACCAGACACTCACAGACGAAGAGAAGAAAACGTTGTTGGGGAGGTATACGGTGAAAGAAACACAACTACCTCGCATGCAAGTTACCGATCCAATTTCTAGATATTATGGGCTGAAGCGTGGACATGTAGTGAAGATCATCCGACCAAGCGAAACTGCTGGAAGATATGTGACTTATAGATATGTTGTTTGA

Protein sequence

MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRYVTYRYVV

Homology

[Close]

BLAST of Cucsat.G10613 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 3.4e-80
Identity = 150/203 (73.89%), Postives = 177/203 (87.19%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNS 64
           EEE+ +L+R++KT++QML+DRGYF+ D EL M+K QF  K G+NMKREDLV  K+KRN++
Sbjct: 4   EEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDN 63

Query: 65  SDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHL 124
           SDQ+Y+FFP+E KVG+K ++ Y  RMKSENVFRAI VVQ NLTPFAR  I+E+ SSK HL
Sbjct: 64  SDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEI-SSKFHL 123

Query: 125 EVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKR 184
           EVFQE E+LVNIKEHVL+PEHQ LT EEKKTLL RYTVKETQLPR+QVTDPI+RY+GLKR
Sbjct: 124 EVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKR 183

Query: 185 GHVVKIIRPSETAGRYVTYRYVV 208
           G VVKIIRPSETAGRYVTYRYVV
Sbjct: 184 GQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of Cucsat.G10613 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.9e-52
Identity = 104/207 (50.24%), Postives = 147/207 (71.01%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGE-----NMKREDLVINKS 64
           EEE  +L+++RKT++Q+  DRGY V   ELD +  +FK +FG+       +R DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS 124
             ++ +DQ++VFFPEEPKVGIK ++ Y  RM+ EN+ RA+ VVQ  +TP A+  + ++ +
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-A 123

Query: 125 SKLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRY 184
            K  LE F + ELL+NI EH L+PEH  +T EE   LL RY ++E+QLPR+Q  DP++RY
Sbjct: 124 PKYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARY 183

Query: 185 YGLKRGHVVKIIRPSETAGRYVTYRYV 207
           +G+KRG VVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of Cucsat.G10613 vs. ExPASy Swiss-Prot
Match: B0BNE2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus OX=10116 GN=Polr2e PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.9e-52
Identity = 104/207 (50.24%), Postives = 147/207 (71.01%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGE-----NMKREDLVINKS 64
           EEE  +L+++RKT++Q+  DRGY V   ELD +  +FK +FG+       +R DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS 124
             ++ +DQ++VFFPEEPKVGIK ++ Y  RM+ EN+ RA+ VVQ  +TP A+  + ++ +
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-A 123

Query: 125 SKLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRY 184
            K  LE F + ELL+NI EH L+PEH  +T EE   LL RY ++E+QLPR+Q  DP++RY
Sbjct: 124 PKYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARY 183

Query: 185 YGLKRGHVVKIIRPSETAGRYVTYRYV 207
           +G+KRG VVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of Cucsat.G10613 vs. ExPASy Swiss-Prot
Match: Q5R587 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=9601 GN=POLR2E PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-51
Identity = 103/207 (49.76%), Postives = 146/207 (70.53%), Query Frame = 0


Query: 5   EEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGE-----NMKREDLVINKS 64
           EEE  +L+++RKT++Q+  DRGY V   ELD +  +F+ +FG+       +R DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS 124
             ++ +DQ++VFFPEEPKVGIK ++ Y  RM+ EN+ RA+ VVQ  +TP A+  + ++ +
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-A 123

Query: 125 SKLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRY 184
            K  LE F + ELL+NI EH L+PEH  +T EE   LL RY ++E QLPR+Q  DP++RY
Sbjct: 124 PKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARY 183

Query: 185 YGLKRGHVVKIIRPSETAGRYVTYRYV 207
           +G+KRG VVKIIRPSETAGRY+TYR V
Sbjct: 184 FGIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of Cucsat.G10613 vs. ExPASy Swiss-Prot
Match: Q54EH2 (DNA-directed RNA polymerases I, II, and III subunit rpabc1 OS=Dictyostelium discoideum OX=44689 GN=polr2e PE=3 SV=2)

HSP 1 Score: 203.0 bits (515), Expect = 3.3e-51
Identity = 107/203 (52.71%), Postives = 139/203 (68.47%), Query Frame = 0


Query: 6   EEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSS 65
           E+I +L ++RKTVLQML DR Y +  +E +  +  F       + RE+L +   K+++ +
Sbjct: 2   EDINRLLQIRKTVLQMLSDREYIITAYEQEYPRESF-------VSRENLTMYCVKKDDPT 61

Query: 66  DQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELS-SSKLHL 125
           D IYVFFP+ PKVG+  ++ YV  MK     RAI VVQ N+TPFA+  +AE S   K+ L
Sbjct: 62  DSIYVFFPDTPKVGVTQIKKYVDIMKDRQANRAIIVVQQNITPFAKQALAEFSQEKKITL 121

Query: 126 EVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKR 185
           E F E ELLVNI  H L+P+H  LT EEK  LL RY +KE+QLPR+Q  DP++RYYGL R
Sbjct: 122 EQFNEAELLVNITHHQLVPKHILLTKEEKLELLTRYKMKESQLPRIQTNDPVARYYGLSR 181

Query: 186 GHVVKIIRPSETAGRYVTYRYVV 208
           GHVVKI+RPSETAGRY+TYR  V
Sbjct: 182 GHVVKIVRPSETAGRYITYRLCV 197

[Close]

BLAST of Cucsat.G10613 vs. NCBI nr
Match: XP_004143184.1 (DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN47027.1 hypothetical protein Csa_020872 [Cucumis sativus])

HSP 1 Score: 403 bits (1036), Expect = 1.33e-141
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY
Sbjct: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207
           GLKRGHVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of Cucsat.G10613 vs. NCBI nr
Match: XP_008456369.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo] >KAA0054557.1 DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 391 bits (1004), Expect = 1.01e-136
Identity = 199/207 (96.14%), Postives = 204/207 (98.55%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYY
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207
           GLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of Cucsat.G10613 vs. NCBI nr
Match: TYK20394.1 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 380 bits (977), Expect = 2.33e-132
Identity = 199/223 (89.24%), Postives = 204/223 (91.48%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQ-------------- 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQ              
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQVSFILFDLIFLKYK 180

Query: 181 --LPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRYVTYRYVV 207
             LPRMQVTDPI+RYYGLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 NILPRMQVTDPIARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 223

[Close]

BLAST of Cucsat.G10613 vs. NCBI nr
Match: XP_008456371.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 [Cucumis melo])

HSP 1 Score: 354 bits (908), Expect = 2.13e-122
Identity = 179/187 (95.72%), Postives = 184/187 (98.40%), Query Frame = 0


Query: 21  MLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI 80
           MLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI
Sbjct: 1   MLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI 60

Query: 81  KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHLEVFQETELLVNIKEHV 140
           KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+K+HLEVFQE EL+VNIKEHV
Sbjct: 61  KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSTKVHLEVFQEAELMVNIKEHV 120

Query: 141 LIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRY 200
           LIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYYGLKRG VVKIIRPSETAGRY
Sbjct: 121 LIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYYGLKRGQVVKIIRPSETAGRY 180

Query: 201 VTYRYVV 207
           VTYRYVV
Sbjct: 181 VTYRYVV 187

[Close]

BLAST of Cucsat.G10613 vs. NCBI nr
Match: XP_018829461.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans regia] >XP_018829463.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans regia] >XP_018829464.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans regia] >XP_041001822.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans microcarpa x Juglans regia] >XP_041001823.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans microcarpa x Juglans regia] >XP_041001824.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans microcarpa x Juglans regia] >KAF5451447.1 hypothetical protein F2P56_026555 [Juglans regia])

HSP 1 Score: 338 bits (868), Expect = 5.20e-116
Identity = 169/207 (81.64%), Postives = 190/207 (91.79%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           M   EEEIT+LFR+R+TV+QMLKDRGYFVGDFE+DM+K QFKNK+GE+MKREDLVINKSK
Sbjct: 1   MTLSEEEITRLFRIRRTVMQMLKDRGYFVGDFEIDMTKEQFKNKYGESMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIY+FFPEEPKVG+K ++TY  RMKSENVFRAI VVQ NLTPFAR+ I E+S+ 
Sbjct: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYSQRMKSENVFRAILVVQQNLTPFARSSINEIST- 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           K HLEVFQE ELL+NIKEHVL+PEHQ LT+EEKKTLL RYTVKETQLPR+QVTDP++RYY
Sbjct: 121 KFHLEVFQEAELLINIKEHVLVPEHQVLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207
           GLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of Cucsat.G10613 vs. ExPASy TrEMBL
Match: A0A0A0KF69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G170410 PE=3 SV=1)

HSP 1 Score: 403 bits (1036), Expect = 6.45e-142
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY
Sbjct: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207
           GLKRGHVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of Cucsat.G10613 vs. ExPASy TrEMBL
Match: A0A5A7UJ12 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G003460 PE=3 SV=1)

HSP 1 Score: 391 bits (1004), Expect = 4.88e-137
Identity = 199/207 (96.14%), Postives = 204/207 (98.55%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYY
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207
           GLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of Cucsat.G10613 vs. ExPASy TrEMBL
Match: A0A1S3C2N2 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496327 PE=3 SV=1)

HSP 1 Score: 391 bits (1004), Expect = 4.88e-137
Identity = 199/207 (96.14%), Postives = 204/207 (98.55%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYY 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYY
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYY 180

Query: 181 GLKRGHVVKIIRPSETAGRYVTYRYVV 207
           GLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 GLKRGQVVKIIRPSETAGRYVTYRYVV 207

[Close]

BLAST of Cucsat.G10613 vs. ExPASy TrEMBL
Match: A0A5D3DA58 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold519G00160 PE=3 SV=1)

HSP 1 Score: 380 bits (977), Expect = 1.13e-132
Identity = 199/223 (89.24%), Postives = 204/223 (91.48%), Query Frame = 0


Query: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSK 60
           MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSK
Sbjct: 1   MVFPEEEITKLFRVRKTVLQMLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSK 60

Query: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSS 120
           RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+
Sbjct: 61  RNNSSDQIYVFFPEEPKVGIKIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSST 120

Query: 121 KLHLEVFQETELLVNIKEHVLIPEHQTLTDEEKKTLLGRYTVKETQ-------------- 180
           K+HLEVFQE EL+VNIKEHVLIPEHQ+LTDEEKKTLLGRYTVKETQ              
Sbjct: 121 KVHLEVFQEAELMVNIKEHVLIPEHQSLTDEEKKTLLGRYTVKETQVSFILFDLIFLKYK 180

Query: 181 --LPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRYVTYRYVV 207
             LPRMQVTDPI+RYYGLKRG VVKIIRPSETAGRYVTYRYVV
Sbjct: 181 NILPRMQVTDPIARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 223

[Close]

BLAST of Cucsat.G10613 vs. ExPASy TrEMBL
Match: A0A1S3C358 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496327 PE=3 SV=1)

HSP 1 Score: 354 bits (908), Expect = 1.03e-122
Identity = 179/187 (95.72%), Postives = 184/187 (98.40%), Query Frame = 0


Query: 21  MLKDRGYFVGDFELDMSKVQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI 80
           MLKDRGYFVGDFELDMSK QFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI
Sbjct: 1   MLKDRGYFVGDFELDMSKAQFKNKFGENMKREDLVINKSKRNNSSDQIYVFFPEEPKVGI 60

Query: 81  KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSSKLHLEVFQETELLVNIKEHV 140
           KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSS+K+HLEVFQE EL+VNIKEHV
Sbjct: 61  KIVETYVGRMKSENVFRAIFVVQLNLTPFARNKIAELSSTKVHLEVFQEAELMVNIKEHV 120

Query: 141 LIPEHQTLTDEEKKTLLGRYTVKETQLPRMQVTDPISRYYGLKRGHVVKIIRPSETAGRY 200
           LIPEHQ+LTDEEKKTLLGRYTVKETQLPRMQVTDPI+RYYGLKRG VVKIIRPSETAGRY
Sbjct: 121 LIPEHQSLTDEEKKTLLGRYTVKETQLPRMQVTDPIARYYGLKRGQVVKIIRPSETAGRY 180

Query: 201 VTYRYVV 207
           VTYRYVV
Sbjct: 181 VTYRYVV 187

The following BLAST results are available for this feature:

BLAST of Cucsat.G10613 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81098	3.4e-80	73.89	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
Q80UW8	3.9e-52	50.24	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10...	[more]
B0BNE2	3.9e-52	50.24	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus ...	[more]
Q5R587	1.1e-51	49.76	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=96...	[more]
Q54EH2	3.3e-51	52.71	DNA-directed RNA polymerases I, II, and III subunit rpabc1 OS=Dictyostelium disc...	[more]

BLAST of Cucsat.G10613 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143184.1	1.33e-141	100.00	DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN47027.1 ...	[more]
XP_008456369.1	1.01e-136	96.14	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cu...	[more]
TYK20394.1	2.33e-132	89.24	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo ...	[more]
XP_008456371.1	2.13e-122	95.72	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 [Cu...	[more]
XP_018829461.1	5.20e-116	81.64	DNA-directed RNA polymerases II and IV subunit 5A-like [Juglans regia] >XP_01882...	[more]

BLAST of Cucsat.G10613 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KF69	6.45e-142	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G170410 PE=3 SV=1	[more]
A0A5A7UJ12	4.88e-137	96.14	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A1S3C2N2	4.88e-137	96.14	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A5D3DA58	1.13e-132	89.24	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A1S3C358	1.03e-122	95.72	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 OS=Cucumis mel...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 4..131 e-value: 5.1E-44 score: 151.7
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 5..135
IPR014381	DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus	PIRSF	PIRSF000747	RPB5	coord: 2..207 e-value: 2.6E-82 score: 273.3
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 132..207 e-value: 5.8E-32 score: 111.2
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 132..207
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 5..90 e-value: 2.1E-29 score: 102.0
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 134..206 e-value: 2.0E-32 score: 110.9
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 1..207
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 1..207
IPR020609	Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit	HAMAP	MF_00025	RNApol_RpoH_RPB5	coord: 134..207 score: 21.908932

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G10613.T1	Cucsat.G10613.T1	mRNA
Cucsat.G10613.T2	Cucsat.G10613.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity