Cucsat.G10569 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G10569
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Citrate synthase
Location	ctg1679: 153575 .. 158439 (-)
RNA-Seq Expression	Cucsat.G10569
Synteny	Cucsat.G10569

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTATTTATTTTCCTAAACTCCTAAAAGCCAATTCAATTCTCACATCTTTTAAATGCGAGAAAGTTGCATTATTTAGTTTCATTTCCGACCTATTTTCAAGGAAAAGACGAAGAAGAAGTTCCTTACAAGCAATTTAGAAAAGAGAAATTGATTAAATTTCGTCGATCGGAAACGGAAACGGAAACAGATTCCAACCGACATGGAATTGTCGCCGTCGAACGTTGCTCGTGGTCGCCTGGCCGTTTTGGCAGCGCATCTGAGCGCTGCCACCTTGGAATCGCCGGTGATGGCTTCGTTACTGGAGGCTAATTGCGTGTCAGCTCGAACCATGCTTCCACCGCCTGAAGCATTGAAGGGTACTTTGACGATTGTTGATGAGAGAACTGGTAAGAGATATCAGGTCCAGATATCCGAAGAAGGCACGATAAAGGCCACTGACTTGAAGAAGGTACACAAATGAATTAATGGATCTGTTGCACGTTTTCCCATAGTTGAGTCGGTTTGTTTGTTTGTTTTGTTGTTATTGATTTAACTCTAGGCTCGTCGGTTGTGCTTGATTAAGCTTTTGTTGTGTTGTTTGATTTCTTGGAATCATCTTCCTGGTGTTAAATGTTAATCGATGGATGTGGTTGTACTGTTTGTTCGTAGATAACTACTGGACCAAATGACAAGGGACTTAAACTGTACGATCCAGGCTATCTCAACACTGCTCCAGTTCGGTCATCCATTAGTTATATTGATGGTGATGAGGGGATCCTTAGGTACAGAGGTTACCCAATTGAGGAACTGGCTGAGAGTAGTACCTATGTGGAAGTTGCATACCTCTTGAGTAACTCACTTCTTTAACAATCTATTCCTTTTGATCTAAGAACATTGTTTTTTGCTTCTGTTCTGTTCATGGATAGTAATATATGTAACGTTTGATTTGTGTTCCAGTGTATGGGAATTTGCCTTCTCAGAGTCAATTGGCAGATTGGGAATTTGCTCTTTCTCAGCATTCTGCTGTACCACAGGGACTTTTGGTGAGATTGCATTTTTCTATTCATATATTTCTTTATATAAATCGCATTGCTCTATTGATTTTTCTGAAGGACTGTTAATTCTCTCTCTTTATCTCAATAGGATATTATTCAAGCAATGCCTCATGATGCACATCCAATGGGTGTTCTTGTTAGTGCAATGAGTGCTCTATCTGTCTTTCATCCAGATGCCAATCCTGCTCTGAGAGTAAGTAGATATTTTTTGTTCTCAGTTGTTTATTGCATATTAATCTTCTGCACCTACTCCTAAACCTTCTCAGTTTTTTGTTGCATGGTAAGTAACTTTTATGTATGTTATCTTTTGTTTAAAAAGTTTTGAGGGGGTTTAGAGAAAAGTTTGGACTCTTGTATAGGTTTTTGTCTAAAGAATCCAAGAGTGCAATTATTCTATTTCTTCCTCAAGAAAAGATAAAATTGAAGAAAATAAATAAAAAAAGGGATGTTCTCTGGGACCCTCCTCTCTTCTAGAAGCTGAGTTTCGGCCCAGCTTAGTTGAAGTACCACTTTAGTTTAATCAACATTAACGAGGAATGGATGAGAAACTATACCTGCTTGAACTGACTAGTTCTTTTTACGCTGTGGATCTTGAAAAAACATTTTTAAGGCATACTGTGGAGAAAGTGATATTTTCTCTCTGTATTCAATTAACATTTAAAACTGCGTAATGTCTTCATTTTATTTCCTACACTTTAACTGAACATAAACTTACTCATACTTTACTAGTATGTAGTTCATTACTTCAATTATCTTCTGATACAGCGGACTTTGTTAAAATGGCATCAGTGGCCAAGAGTAGGTGGGAAATCCATCCCAACGGAAGGAAGAAATGTTTGTTGTAATTTATTTATTTTTATTGAAAGCAATAAAACACGAAACATCAACTTACGTGAAAACCTGAGTATCGGGAGAAAAACCATGGTTTTTTTGTTGTTATTATTTTCTGATAAAATACAATAGGTACAAGAGGGGGAAATAACTGAAATAGTAGAAAGAGATAAAAAAGGAAAAAATATTTAGGGCAAAACTTCCAATGGGCTAAAATTTAACATAATTTTGTCTTTGTATGGTATACAACAGAAGTGGAAAATACCACATAAAAACCTAAGGAAGTTGATTAAAATATCATGATCAACTATCCTAACAATTGTAATACTCTTGATCAAATCTTCGTTGTAATCTTATTTGTTATCATTGTTTGTACACAAATCTATCTTTTAAGACTTTTTAATCCATGTGAAAGGATTTTATTTTTAGCTATGCATCATTGTTTTGCTGTAGGGACAAGATCTGTACAAGTCTAAGCAAGTGAGAGACAAACAAATAGCACGTATTATAGGGAAGGTATGCTTTCTATGAACATTTTGTCAGCTGAGATAGTTTTATTACTGTTACTGTTATTGCCATGTTATTTTTTTGATTTGTTCGTCAATTTGATTTTGTATTGAAATACATACTGCAGTTTGAGAGTTCCATCTATTTCCTTCTAGATGTACAGAACTACCAACTAATTTGATGGTATATTCTCTGAATTTATAGGCTCCCACCATTGCAGCAGCTGCATATCTTAGATTGGCTGGAAGACCTCCAGTTCTCCCTTCCAGCAACCTTTCTTATTCAGAGAATTTCTTGTACATGCTTGATTCTCTGTAAGTTGCCTAGTTATCTTTTTTTCTTTCTTTTCCCCCTCATGAAAATATGAGGATAGGTTCTATTTCCTAATAGAAATGTCAATGTCTTTACCTTTGCTAAATTGTATTCTCGTGTGACTTGAATAATATTGTACTCACAAAACTATCAGACTTCAAGATAAATATGAACTGAATTGAGGAAGTAGAAAAACAAAAGCTTTGGGCCTGAACAAGACTTTTGTTCTATTCAGGGGTAATAGGTCTTATAAACCCAATCCTCGGCTTGCTCGAGTCATAGACATTCTATTCATCCTCCATGCAGAACATGAAATGAACTGCTCTACATCTGCTGCTCGCCATCTAGCATCAAGGTAGACAGCTTTATATCAATGACCTGTCTTAATTATCACATCTCTTATTCCCGTCTGATAGCATTTTTCTTTTGGCTTTTCAGTGGTGTTGATGTGTTCACTGCTCTTTCTGGAGCTGTCGGAGCACTGTATGGCCCTCTTCATGGTGGGGCCAATGAGGTATGCACAAAAACAATTTTTTCTCAGCGATTGGAATAAGCTTGCTTCCAACTGAGTTGCTTATGGGGTTCTATCCATGGATTAGACACCACCACTTGGCTCTTTTTGACATTTGTTTGGTGTGTTTCTTTTGTTTTCCTAATGATTGTAGGCTGTACTTAAAATGTTAAGTGAGATTGGAACTGTTGATAACATTCCAGGATTCATCGAGGGTGTTAAAAATAGGTAATTGTCACTTTGTGCGAATAAGCTAAGTTTTTTACTTGGTGGATCTCTTTTTGTTGTGGAAGAATAGGTAGACGCCATTTTGGGCCATACTTCCCAAGGAGCACTTTTCAGTTTTTCTTTTCTTTTCTTTTATTTTAATAGATTTCTCACATTATTTTGTTCTCCAGGAAGAGAAAGATGTCAGGTTTTGGCCATAGGGTGTATAAGAACTACGATCCTAGAGCAAAGGTTATAAGAAAACTTGCGGAAGAAGTATTTTCCATTGTTGGCCGGGATCCTCTAATTGAGGTATTTGTCTTACAACTGTTTCCATGTGAATATGCAATCTGATCTCTCTCTAAGAGTGAGCTACGATTTCTTTTTTAAAAGTGTTTAACAATAATTATTATGCCATATAGGTGGCTGTTGCACTGGAGAAAGCTGCCCTTTCAGATGAGTATTTTGTCAAGAGGAAGTTGTATCCAAATGTTGACTTTTACTCCGGATTAATATACAGGTGGGAAGGTGGTTATTTTAATTTTCTAATGAAAAGATGTAATTGTTCCATCTCTCATGAAGTCGATAACTGATAATGTGTTTGATCAGGGCTATGGGATTTCCACCTGAGTTTTTCACCGTGCTGTTTGCAATCCCTCGAATGGCTGGATACTTGGCACATTGGCGAGAATCTCTCGACGATCCTGACACTAAGATAATTCGACCTCAACAGGTTAGTTCTCTGCTCGCCACATCTTTGTAACTTTATCTTTTCAGTTCTCAAACTAATAGATTGGTTTGATTTCCTTCCTTAGATAGCTTGAAATTTTGTATAATGCTAGCAAGTAGCAATGAGTTCAATTAACCTTATTCTTGGTGTATTCAGGTCTACACTGGGGAATGGCTGCGACATTACATTCCACCCAAAGAACGACTGGTACCAGCCAAAGCAGACAGGCTCGGTCAGGTTTCCGTTTCCAATGCCTCAAAGCGCAGACTATCTGGATCAGGGATCTAGACTATTTGGGATGCATACATATCAAATGGGAGAATAAAACAAACGCATCAAAGGGAAGGTAAAGCGTTGGTTGGCCAAATTACACTGCCTGAATATGAAACAAAGGAAAATATAAGAGATAGGCCCCCTTCTACAAAGAATAAAGGCCACTCTGTCATTTATAGATAGGGATTTTCTATTGTAAGTTATAATTTCCATCATTTTGGAGAAACTTATGTTCTATCACTTCTTTCCATTGTAAAAAAAACAAAAAAACTATTTATATTTATGAAAATGAATAGTTATTGAAGTTTGTTAGTTGCTGCGGTGATGTTTTGTTTGTGTGGATTCATACATCTATTTGAATCTATTGAATTGAGAAATATGAAACGATTGAGCAATAGAAATTCTCTAACAACTCCACCTTTGTTGTATATGCATACATCACTTAGAAGGAATAAATTT

Coding sequence (CDS)

ATGGAATTGTCGCCGTCGAACGTTGCTCGTGGTCGCCTGGCCGTTTTGGCAGCGCATCTGAGCGCTGCCACCTTGGAATCGCCGGTGATGGCTTCGTTACTGGAGGCTAATTGCGTGTCAGCTCGAACCATGCTTCCACCGCCTGAAGCATTGAAGGGTACTTTGACGATTGTTGATGAGAGAACTGGTAAGAGATATCAGGTCCAGATATCCGAAGAAGGCACGATAAAGGCCACTGACTTGAAGAAGATAACTACTGGACCAAATGACAAGGGACTTAAACTGTACGATCCAGGCTATCTCAACACTGCTCCAGTTCGGTCATCCATTAGTTATATTGATGGTGATGAGGGGATCCTTAGGTACAGAGGTTACCCAATTGAGGAACTGGCTGAGAGTAGTACCTATGTGGAAGTTGCATACCTCTTGATGTATGGGAATTTGCCTTCTCAGAGTCAATTGGCAGATTGGGAATTTGCTCTTTCTCAGCATTCTGCTGTACCACAGGGACTTTTGGATATTATTCAAGCAATGCCTCATGATGCACATCCAATGGGTGTTCTTGTTAGTGCAATGAGTGCTCTATCTGTCTTTCATCCAGATGCCAATCCTGCTCTGAGAGTAAGTAGATATTTTTTGTTCTCAGTTGTTTATTGCATATTAATCTTCTGCACCTACTCCTAA

Protein sequence

MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDERTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGILRYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPHDAHPMGVLVSAMSALSVFHPDANPALRVSRYFLFSVVYCILIFCTYS

Homology

[Close]

BLAST of Cucsat.G10569 vs. ExPASy Swiss-Prot
Match: P49299 (Citrate synthase, glyoxysomal OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 1.7e-104
Identity = 193/207 (93.24%), Postives = 201/207 (97.10%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MELSPSNVAR RLAVLAAHLSAA+LE PVMAS LEA+CVSA+TM+ PPE +KGTLTIVDE
Sbjct: 5   MELSPSNVARHRLAVLAAHLSAASLEPPVMASSLEAHCVSAQTMVAPPELVKGTLTIVDE 64

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQ+SEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGD GIL
Sbjct: 65  RTGKRYQVQVSEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDLGIL 124

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGL+DIIQAMPH
Sbjct: 125 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLVDIIQAMPH 184

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 208
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 185 DAHPMGVLVSAMSALSVFHPDANPALR 211

[Close]

BLAST of Cucsat.G10569 vs. ExPASy Swiss-Prot
Match: Q9SJH7 (Citrate synthase 3, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=CSY3 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 3.0e-82
Identity = 153/202 (75.74%), Postives = 173/202 (85.64%), Query Frame = 0


Query: 6   SNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDERTGKR 65
           S   R RLAVL+ HLS    +SP     +E  C SA T + P  +LKGTLTIVDERTGK 
Sbjct: 4   SERVRARLAVLSGHLSEGKQDSPA----IERWCTSADTSVAPLGSLKGTLTIVDERTGKN 63

Query: 66  YQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGILRYRGY 125
           Y+V +S++GT+KA D KKI TG  DKGLKLYDPGYLNTAPVRSSISYIDGDEGILRYRGY
Sbjct: 64  YKVPVSDDGTVKAVDFKKIVTGKEDKGLKLYDPGYLNTAPVRSSISYIDGDEGILRYRGY 123

Query: 126 PIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPHDAHPM 185
           PIEE+AE+ST++EVAYLLMYGNLPS+SQL+DWEFA+SQHSAVPQG+LDIIQ+MPHDAHPM
Sbjct: 124 PIEEMAENSTFLEVAYLLMYGNLPSESQLSDWEFAVSQHSAVPQGVLDIIQSMPHDAHPM 183

Query: 186 GVLVSAMSALSVFHPDANPALR 208
           GVLVSAMSALS+FHPDANPALR
Sbjct: 184 GVLVSAMSALSIFHPDANPALR 201

[Close]

BLAST of Cucsat.G10569 vs. ExPASy Swiss-Prot
Match: Q9LXS6 (Citrate synthase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=CSY2 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 9.8e-81
Identity = 153/202 (75.74%), Postives = 170/202 (84.16%), Query Frame = 0


Query: 6   SNVARGRLAVLAAHLSAA-TLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDERTGK 65
           S   + RLAVL AHL+ + T+    +   +   C SA     P  +LKG LTIVDERTGK
Sbjct: 4   SQRVKARLAVLTAHLAVSDTVGLEQVLPAIAPWCTSAHITAAPHGSLKGNLTIVDERTGK 63

Query: 66  RYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGILRYRG 125
           +YQV +SE GT+KA DLKKITTG +DKGLKLYDPGYLNTAPVRSSI YIDGDEGILRYRG
Sbjct: 64  KYQVPVSEHGTVKAVDLKKITTGKDDKGLKLYDPGYLNTAPVRSSICYIDGDEGILRYRG 123

Query: 126 YPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPHDAHP 185
           YPIEELAESST++EVAYLLMYGNLPSQSQLADWEF +SQHSAVPQG+LDIIQ+MPHDAHP
Sbjct: 124 YPIEELAESSTFIEVAYLLMYGNLPSQSQLADWEFTVSQHSAVPQGVLDIIQSMPHDAHP 183

Query: 186 MGVLVSAMSALSVFHPDANPAL 207
           MGVLVSAMSALS+FHPDANPAL
Sbjct: 184 MGVLVSAMSALSIFHPDANPAL 205

[Close]

BLAST of Cucsat.G10569 vs. ExPASy Swiss-Prot
Match: Q9LXS7 (Citrate synthase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=CSY1 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 6.8e-74
Identity = 141/201 (70.15%), Postives = 162/201 (80.60%), Query Frame = 0


Query: 6   SNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDERTGKR 65
           S  AR RLAVL AHL+ +  E   +   +E  C SA     P  +LKG L IVDERTG  
Sbjct: 4   SERARARLAVLNAHLTVS--EPNQVLPAIEPWCTSAHITAAPHGSLKGNLKIVDERTGNE 63

Query: 66  YQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGILRYRGY 125
           YQV +SE GT+K  DLKKITTG +DKGL LYDPGYLNTAPVRSSISYIDGDEGILRYRGY
Sbjct: 64  YQVPVSEHGTVKTVDLKKITTGKDDKGLNLYDPGYLNTAPVRSSISYIDGDEGILRYRGY 123

Query: 126 PIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPHDAHPM 185
           P+EELAE STY EV YLL+YGNLPSQ QLADWEFA+SQ+SAVPQG+LD+IQ+MP+D HP+
Sbjct: 124 PVEELAEKSTYTEVTYLLIYGNLPSQRQLADWEFAISQNSAVPQGVLDMIQSMPNDVHPV 183

Query: 186 GVLVSAMSALSVFHPDANPAL 207
           G LV+AMSALS+F+PDANP+L
Sbjct: 184 GALVTAMSALSIFYPDANPSL 202

[Close]

BLAST of Cucsat.G10569 vs. ExPASy Swiss-Prot
Match: Q86AV6 (Citrate synthase OS=Dictyostelium discoideum OX=44689 GN=gltA PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.4e-42
Identity = 79/155 (50.97%), Postives = 115/155 (74.19%), Query Frame = 0


Query: 52  KGTLTIVDERTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSIS 111
           K TLT++D RTGK Y++ I+ E T+K+ D + I     D G  +YDPGY NTA  +S I+
Sbjct: 16  KNTLTVIDNRTGKSYEIPINHE-TVKSIDFRAIKEQSIDFGTMIYDPGYYNTAVCKSQIT 75

Query: 112 YIDGDEGILRYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGL 171
           YIDGD GIL YRGYPIE+LAE S+++EV+YLL+YG+LPS+ Q   W   +  H+ + + L
Sbjct: 76  YIDGDRGILEYRGYPIEQLAEKSSFLEVSYLLIYGDLPSKEQSNLWNTKIMNHTFIHENL 135

Query: 172 LDIIQAMPHDAHPMGVLVSAMSALSVFHPDANPAL 207
           + ++++  +DAHPMG+L+S++SA+S F+P+ANPAL
Sbjct: 136 ISMMKSFRYDAHPMGMLISSLSAMSTFYPEANPAL 169

[Close]

BLAST of Cucsat.G10569 vs. NCBI nr
Match: XP_011657098.1 (citrate synthase, glyoxysomal [Cucumis sativus] >KGN47018.1 hypothetical protein Csa_020878 [Cucumis sativus])

HSP 1 Score: 405 bits (1042), Expect = 1.27e-137
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE
Sbjct: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL
Sbjct: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH
Sbjct: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207

[Close]

BLAST of Cucsat.G10569 vs. NCBI nr
Match: XP_008456386.1 (PREDICTED: citrate synthase, glyoxysomal [Cucumis melo] >KAA0054523.1 citrate synthase, glyoxysomal [Cucumis melo var. makuwa] >TYJ98273.1 citrate synthase, glyoxysomal [Cucumis melo var. makuwa])

HSP 1 Score: 388 bits (996), Expect = 1.14e-130
Identity = 199/207 (96.14%), Postives = 202/207 (97.58%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MEL PSNVAR RLAVLAAHLSAATLESP+M S LEANCVSARTM+PPPE LKGTLTIVDE
Sbjct: 1   MEL-PSNVARSRLAVLAAHLSAATLESPLMPSSLEANCVSARTMVPPPEILKGTLTIVDE 60

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL
Sbjct: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGLLDIIQAMPH
Sbjct: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLLDIIQAMPH 180

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 181 DAHPMGVLVSAMSALSVFHPDANPALR 206

[Close]

BLAST of Cucsat.G10569 vs. NCBI nr
Match: XP_038900376.1 (citrate synthase, glyoxysomal [Benincasa hispida])

HSP 1 Score: 384 bits (985), Expect = 6.17e-129
Identity = 196/207 (94.69%), Postives = 199/207 (96.14%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           ME SPSNVAR RLAVLAAHLSAATLE PVMAS LEANCVSARTM+ PPE  KGTLTIVDE
Sbjct: 5   MESSPSNVARSRLAVLAAHLSAATLEPPVMASSLEANCVSARTMVAPPERFKGTLTIVDE 64

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQISEEGTIKATDLKKI+TG NDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL
Sbjct: 65  RTGKRYQVQISEEGTIKATDLKKISTGANDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 124

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGLLDIIQAMPH
Sbjct: 125 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLLDIIQAMPH 184

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 185 DAHPMGVLVSAMSALSVFHPDANPALR 211

[Close]

BLAST of Cucsat.G10569 vs. NCBI nr
Match: XP_022944344.1 (citrate synthase, glyoxysomal [Cucurbita moschata] >XP_023512768.1 citrate synthase, glyoxysomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382 bits (980), Expect = 3.52e-128
Identity = 194/207 (93.72%), Postives = 201/207 (97.10%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MELSPSNVAR RLAVLAAHLSAA+LE PVMAS LEA+CVSA+TM+ PPE +KGTLTIVDE
Sbjct: 5   MELSPSNVARHRLAVLAAHLSAASLEPPVMASSLEAHCVSAQTMVAPPELVKGTLTIVDE 64

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQ+SEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGD GIL
Sbjct: 65  RTGKRYQVQVSEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDLGIL 124

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGLLDIIQAMPH
Sbjct: 125 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLLDIIQAMPH 184

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 185 DAHPMGVLVSAMSALSVFHPDANPALR 211

[Close]

BLAST of Cucsat.G10569 vs. NCBI nr
Match: XP_022985955.1 (citrate synthase, glyoxysomal [Cucurbita maxima] >P49299.1 RecName: Full=Citrate synthase, glyoxysomal; AltName: Full=GCS; Flags: Precursor [Cucurbita maxima] >pir|S53007| citrate synthase - cucurbit >BAA07328.1 glyoxysomal citrate synthase [Cucurbita hybrid cultivar])

HSP 1 Score: 380 bits (977), Expect = 1.00e-127
Identity = 193/207 (93.24%), Postives = 201/207 (97.10%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MELSPSNVAR RLAVLAAHLSAA+LE PVMAS LEA+CVSA+TM+ PPE +KGTLTIVDE
Sbjct: 5   MELSPSNVARHRLAVLAAHLSAASLEPPVMASSLEAHCVSAQTMVAPPELVKGTLTIVDE 64

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQ+SEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGD GIL
Sbjct: 65  RTGKRYQVQVSEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDLGIL 124

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGL+DIIQAMPH
Sbjct: 125 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLVDIIQAMPH 184

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 185 DAHPMGVLVSAMSALSVFHPDANPALR 211

[Close]

BLAST of Cucsat.G10569 vs. ExPASy TrEMBL
Match: A0A0A0KC13 (Citrate synthase OS=Cucumis sativus OX=3659 GN=Csa_6G169340 PE=3 SV=1)

HSP 1 Score: 405 bits (1042), Expect = 6.15e-138
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE
Sbjct: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL
Sbjct: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH
Sbjct: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207

[Close]

BLAST of Cucsat.G10569 vs. ExPASy TrEMBL
Match: A0A5D3BGA7 (Citrate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold203G00170 PE=3 SV=1)

HSP 1 Score: 388 bits (996), Expect = 5.52e-131
Identity = 199/207 (96.14%), Postives = 202/207 (97.58%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MEL PSNVAR RLAVLAAHLSAATLESP+M S LEANCVSARTM+PPPE LKGTLTIVDE
Sbjct: 1   MEL-PSNVARSRLAVLAAHLSAATLESPLMPSSLEANCVSARTMVPPPEILKGTLTIVDE 60

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL
Sbjct: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGLLDIIQAMPH
Sbjct: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLLDIIQAMPH 180

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 181 DAHPMGVLVSAMSALSVFHPDANPALR 206

[Close]

BLAST of Cucsat.G10569 vs. ExPASy TrEMBL
Match: A0A1S3C3T1 (Citrate synthase OS=Cucumis melo OX=3656 GN=LOC103496336 PE=3 SV=1)

HSP 1 Score: 388 bits (996), Expect = 5.52e-131
Identity = 199/207 (96.14%), Postives = 202/207 (97.58%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MEL PSNVAR RLAVLAAHLSAATLESP+M S LEANCVSARTM+PPPE LKGTLTIVDE
Sbjct: 1   MEL-PSNVARSRLAVLAAHLSAATLESPLMPSSLEANCVSARTMVPPPEILKGTLTIVDE 60

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL
Sbjct: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGLLDIIQAMPH
Sbjct: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLLDIIQAMPH 180

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 181 DAHPMGVLVSAMSALSVFHPDANPALR 206

[Close]

BLAST of Cucsat.G10569 vs. ExPASy TrEMBL
Match: A0A6J1FWJ1 (Citrate synthase OS=Cucurbita moschata OX=3662 GN=LOC111448813 PE=3 SV=1)

HSP 1 Score: 382 bits (980), Expect = 1.70e-128
Identity = 194/207 (93.72%), Postives = 201/207 (97.10%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MELSPSNVAR RLAVLAAHLSAA+LE PVMAS LEA+CVSA+TM+ PPE +KGTLTIVDE
Sbjct: 5   MELSPSNVARHRLAVLAAHLSAASLEPPVMASSLEAHCVSAQTMVAPPELVKGTLTIVDE 64

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQ+SEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGD GIL
Sbjct: 65  RTGKRYQVQVSEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDLGIL 124

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGLLDIIQAMPH
Sbjct: 125 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLLDIIQAMPH 184

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 185 DAHPMGVLVSAMSALSVFHPDANPALR 211

[Close]

BLAST of Cucsat.G10569 vs. ExPASy TrEMBL
Match: A0A6J1JCQ2 (Citrate synthase OS=Cucurbita maxima OX=3661 GN=LOC111483841 PE=3 SV=1)

HSP 1 Score: 380 bits (977), Expect = 4.85e-128
Identity = 193/207 (93.24%), Postives = 201/207 (97.10%), Query Frame = 0


Query: 1   MELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDE 60
           MELSPSNVAR RLAVLAAHLSAA+LE PVMAS LEA+CVSA+TM+ PPE +KGTLTIVDE
Sbjct: 5   MELSPSNVARHRLAVLAAHLSAASLEPPVMASSLEAHCVSAQTMVAPPELVKGTLTIVDE 64

Query: 61  RTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDEGIL 120
           RTGKRYQVQ+SEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGD GIL
Sbjct: 65  RTGKRYQVQVSEEGTIKATDLKKITTGPNDKGLKLYDPGYLNTAPVRSSISYIDGDLGIL 124

Query: 121 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMPH 180
           RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFA+SQHSAVPQGL+DIIQAMPH
Sbjct: 125 RYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLVDIIQAMPH 184

Query: 181 DAHPMGVLVSAMSALSVFHPDANPALR 207
           DAHPMGVLVSAMSALSVFHPDANPALR
Sbjct: 185 DAHPMGVLVSAMSALSVFHPDANPALR 211

The following BLAST results are available for this feature:

BLAST of Cucsat.G10569 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49299	1.7e-104	93.24	Citrate synthase, glyoxysomal OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q9SJH7	3.0e-82	75.74	Citrate synthase 3, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=CSY3 PE=2 SV=...	[more]
Q9LXS6	9.8e-81	75.74	Citrate synthase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=CSY2 PE=2 SV=...	[more]
Q9LXS7	6.8e-74	70.15	Citrate synthase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=CSY1 PE=2 SV=...	[more]
Q86AV6	2.4e-42	50.97	Citrate synthase OS=Dictyostelium discoideum OX=44689 GN=gltA PE=3 SV=1	[more]

BLAST of Cucsat.G10569 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011657098.1	1.27e-137	100.00	citrate synthase, glyoxysomal [Cucumis sativus] >KGN47018.1 hypothetical protein...	[more]
XP_008456386.1	1.14e-130	96.14	PREDICTED: citrate synthase, glyoxysomal [Cucumis melo] >KAA0054523.1 citrate sy...	[more]
XP_038900376.1	6.17e-129	94.69	citrate synthase, glyoxysomal [Benincasa hispida]	[more]
XP_022944344.1	3.52e-128	93.72	citrate synthase, glyoxysomal [Cucurbita moschata] >XP_023512768.1 citrate synth...	[more]
XP_022985955.1	1.00e-127	93.24	citrate synthase, glyoxysomal [Cucurbita maxima] >P49299.1 RecName: Full=Citrate...	[more]

BLAST of Cucsat.G10569 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KC13	6.15e-138	100.00	Citrate synthase OS=Cucumis sativus OX=3659 GN=Csa_6G169340 PE=3 SV=1	[more]
A0A5D3BGA7	5.52e-131	96.14	Citrate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold203G001...	[more]
A0A1S3C3T1	5.52e-131	96.14	Citrate synthase OS=Cucumis melo OX=3656 GN=LOC103496336 PE=3 SV=1	[more]
A0A6J1FWJ1	1.70e-128	93.72	Citrate synthase OS=Cucurbita moschata OX=3662 GN=LOC111448813 PE=3 SV=1	[more]
A0A6J1JCQ2	4.85e-128	93.24	Citrate synthase OS=Cucurbita maxima OX=3661 GN=LOC111483841 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002020	Citrate synthase	PRINTS	PR00143	CITRTSNTHASE	coord: 177..197 score: 55.86 coord: 259..273 score: 47.82 coord: 124..152 score: 44.63 coord: 48..61 score: 43.68 coord: 102..117 score: 37.98 coord: 239..255 score: 56.0
IPR002020	Citrate synthase	PFAM	PF00285	Citrate_synt	coord: 1..291 e-value: 2.0E-105 score: 352.8
IPR002020	Citrate synthase	PANTHER	PTHR11739	CITRATE SYNTHASE	coord: 1..319
IPR024176	Citrate synthase, bacterial-type	PIRSF	PIRSF001369	Citrate_synth	coord: 1..309 e-value: 1.3E-107 score: 357.9
IPR016143	Citrate synthase-like, small alpha subdomain	GENE3D	1.10.230.10		coord: 151..256 e-value: 7.3E-108 score: 362.5
IPR016142	Citrate synthase-like, large alpha subdomain	GENE3D	1.10.580.10	Citrate Synthase, domain 1	coord: 1..290 e-value: 7.3E-108 score: 362.5
None	No IPR available	PANTHER	PTHR11739:SF32	CITRATE SYNTHASE	coord: 1..319
IPR019810	Citrate synthase active site	PROSITE	PS00480	CITRATE_SYNTHASE	coord: 184..196
IPR036969	Citrate synthase superfamily	SUPERFAMILY	48256	Citrate synthase	coord: 1..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G10569.T8	Cucsat.G10569.T8	mRNA
Cucsat.G10569.T3	Cucsat.G10569.T3	mRNA
Cucsat.G10569.T2	Cucsat.G10569.T2	mRNA
Cucsat.G10569.T1	Cucsat.G10569.T1	mRNA
Cucsat.G10569.T6	Cucsat.G10569.T6	mRNA
Cucsat.G10569.T4	Cucsat.G10569.T4	mRNA
Cucsat.G10569.T7	Cucsat.G10569.T7	mRNA
Cucsat.G10569.T5	Cucsat.G10569.T5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006099	tricarboxylic acid cycle
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0004108	citrate (Si)-synthase activity
molecular_function	GO:0046912	acyltransferase, acyl groups converted into alkyl on transfer