Cucsat.G10259 (gene) Cucumber (B10) v3

Overview
NameCucsat.G10259
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRING-type domain-containing protein
Locationctg1674: 45786 .. 47889 (-)
RNA-Seq ExpressionCucsat.G10259
SyntenyCucsat.G10259
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCATTTAGTTATTTTCAAATTAAAACTTCACAAAAAGAAAAAAGGAAAAGAAAAAATAAGTATTGGAAACCTTCTTCATCCTCTCTCCTTCAAAACTCAATCGGCATCCTTGGCGTTGGTTTGGCGGAGAGAAAATGATGGGGGAAGATCGCGGCCTTCCTTCTCATTGTTTTTCTCATCTTCTTTTCTTTTTCTGAATCCTTCCCATATTCATCCAAAGGCATGCTCATCCACTTTCTCTAGAGGTAAATTCGTATTTTACATTTTCCATTCTTCATCTTTGATTTTGATTTTGATTTTGATTTTCTTTCATATTTAGTTCTATTTTCATTGGGTTTCCTTCCTTTCAGTTTCTGTTCACACGTTCCGTTGGAATGTTGGGGGCGATTGATTTTGTTGTTGTTGATAATTTTGTGAATTATTCATATCCAAACTCTGATCGAAGTTTAGCTTTCGAATATGCATCTTGGAGCAAGTGTATTGCCTCCGCACCAAATTTCTTTCTTTTTCTCTATTCTGGAATATGATGTTTATATATATCTGTTGCAATGTTTGTCAACATTGTTCGCTTTTGATTATTTTGTGACACTGGGTAAATTGTTTTTCTGCTTTTTTTTTTTTTCCTTTTGTTCTTTTGTGTTTTATTTATTTTTGGCAGTAATCTACTTATGGATTGTATATTCAAAATTGTTGAATTGGCGGTAGTCATCTGATGCCAGCAGAGAAGTTTTGTTGTGATTCTATTGTTTGTTCATAGTGTTGAAACCTTTGGAAGAAAAATGTCCTTATTTCTCGAAAATTTTCCATTCAAGTGATGCTTTGGTATGGTTTAATTAGATTTGGTTTTTAGTTTCTGAAAATTAAGCCTATATAAGATCTTCTTTTACCTTTTAACTATTGTTCTTTGTTGTCTAACATTCTTAAAATTCAAGTCAAAATTTGAAAATTAAAAAAATATTTATAAACTTGTTTGTTTTTTTAACTTTGGGTAAGACTTTAAATTGTGGTAAGAAAATGAGAGGAAATAAGCTTCATTTTCAAAAACTAAAAACTAAAAACCAAATTTTGGTAACTAATTTGTCTTTTAGTACAAACCATTCCGTCAATTTCTATTCTCTAGAAAGGTACCATAGTTTTTAAAATATCGTCTTTAAAGATATTATTACTAAGATGTATAATATTCTTTGATTGGATCTCTAATAATTTGGAAGAACTGTTTCTTTGTGGGAGGCTTCGTAGTTTTTCTTTCATAGATGAACATCTATTTTGCAGATGTCATGTTGATGGCATATTTAAACAATTCTTCTGTCGTCCCTTTTGGAACATCCGATAAAATTGAAACAATTCTTCTGTCAGGATGTGGAAGTTGGCATCGAACGCCATTGCTGGCATTCGATCTAAGAAAAACTCAGATGTATCTAAGCAAGTTTTCTGGGAGTGCTCGGATGACGAGGCTTGCTCGGAAGCCAGTAGAGATGAAGAATTGGAGTGCCCTATATGCTGGGAATCTTTTAACATTGTGGAGAATGTACCTTATGTCTTATGGTGTGGCCATTCCTTGTGCAAAAATTGTGTATTAGGCCTTCAAGGGTCTGTGCTGAAACTAGCTACTCGCCAAATCCGAATCCCAATCGTCATTTCGTGCCCTTGGTGTCATCAGCTGTCACTAAGGGTGGTGTACAAGGGGAACCTGAAGTTTCCCAGCAAGAACTTTTTTCTTCTTTGGATGGTTGAGAGCTTTAATGGCAATGAGGGAAAACTTGACCATTCCTTCAATTCGGATAATCACCCACTTTTTTCTTTGCCATCGGGTACCGGTACCGGTACCGTTACTTCTGAGAGGCGGCGATTGTTTTTCTATAATCATTTGGATTTCGTCGTCCATTTAATCTTCAAGTTTCTACTTCTTGTCATATTTGTTCTGATAGTTGTTTTTGTAATCCCTGGAAGTGCACTCATCTTGCTGCTTTATCTGCTAATCACTTTACTTTTTGCATTGCCATCTTTGCTGATATTTTACCTTGCATTCCATGCTTTGGAAAAGCTTATGAATGATATAACTTCATGAAATGTTTTAACCAATCTCATTTGGTGAATTTCAA

Coding sequence (CDS)

ATGTGGAAGTTGGCATCGAACGCCATTGCTGGCATTCGATCTAAGAAAAACTCAGATGTATCTAAGCAAGTTTTCTGGGAGTGCTCGGATGACGAGGCTTGCTCGGAAGCCAGTAGAGATGAAGAATTGGAGTGCCCTATATGCTGGGAATCTTTTAACATTGTGGAGAATGTACCTTATGTCTTATGGTGTGGCCATTCCTTGTGCAAAAATTGTGTATTAGGCCTTCAAGGGTCTGTGCTGAAACTAGCTACTCGCCAAATCCGAATCCCAATCGTCATTTCGTGCCCTTGGTGTCATCAGCTGTCACTAAGGGTGGTGTACAAGGGGAACCTGAAGTTTCCCAGCAAGAACTTTTTTCTTCTTTGGATGGTTGAGAGCTTTAATGGCAATGAGGGAAAACTTGACCATTCCTTCAATTCGGATAATCACCCACTTTTTTCTTTGCCATCGGGTACCGGTACCGGTACCGTTACTTCTGAGAGGCGGCGATTGTTTTTCTATAATCATTTGGATTTCGTCGTCCATTTAATCTTCAAGTTTCTACTTCTTGTCATATTTGTTCTGATAGTTGTTTTTGTAATCCCTGGAAGTGCACTCATCTTGCTGCTTTATCTGCTAATCACTTTACTTTTTGCATTGCCATCTTTGCTGATATTTTACCTTGCATTCCATGCTTTGGAAAAGCTTATGAATGATATAACTTCATGA

Protein sequence

MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPYVLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFFLLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSERRRLFFYNHLDFVVHLIFKFLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHALEKLMNDITS
Homology
BLAST of Cucsat.G10259 vs. ExPASy Swiss-Prot
Match: Q9H0X6 (RING finger protein 208 OS=Homo sapiens OX=9606 GN=RNF208 PE=1 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 2.4e-05
Identity = 27/69 (39.13%), Postives = 35/69 (50.72%), Query Frame = 0

Query: 33  ACSEASRDEELECPICWESFNIVENVPYVLWCGHSLCKNCVLGLQGSVLKLATRQIRIPI 92
           A S A+  E LECP C  S+N+ +  P VL C HS+C+ C+  L  S  K          
Sbjct: 131 AASSAAAGEPLECPTCGHSYNVTQRRPRVLSCLHSVCEQCLQILYESCPKYK-------- 190

Query: 93  VISCPWCHQ 102
            ISCP C +
Sbjct: 191 FISCPTCRR 191

BLAST of Cucsat.G10259 vs. ExPASy Swiss-Prot
Match: Q8K0W3 (RING finger protein 208 OS=Mus musculus OX=10090 GN=Rnf208 PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 9.1e-05
Identity = 24/61 (39.34%), Postives = 32/61 (52.46%), Query Frame = 0

Query: 41  EELECPICWESFNIVENVPYVLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCH 100
           E LECP C  ++N+ +  P VL C HS+C+ C+  L  S  K           ISCP CH
Sbjct: 143 EPLECPTCGHTYNVTQRRPRVLSCLHSVCEQCLQILYESCPKYK--------FISCPTCH 195

Query: 101 Q 102
           +
Sbjct: 203 R 195

BLAST of Cucsat.G10259 vs. ExPASy Swiss-Prot
Match: Q96D59 (E3 ubiquitin-protein ligase RNF183 OS=Homo sapiens OX=9606 GN=RNF183 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 2.7e-04
Identity = 24/66 (36.36%), Postives = 34/66 (51.52%), Query Frame = 0

Query: 36  EASRDEELECPICWESFNIVENVPYVLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVIS 95
           +  R+ E ECP+CW  FN   + P +L C HS C  C+  L  S++  A R+      + 
Sbjct: 4   QQGRELEAECPVCWNPFNNTFHTPKMLDCCHSFCVECLAHL--SLVTPARRR------LL 61

Query: 96  CPWCHQ 102
           CP C Q
Sbjct: 64  CPLCRQ 61

BLAST of Cucsat.G10259 vs. ExPASy Swiss-Prot
Match: Q13049 (E3 ubiquitin-protein ligase TRIM32 OS=Homo sapiens OX=9606 GN=TRIM32 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 2.7e-04
Identity = 22/63 (34.92%), Postives = 34/63 (53.97%), Query Frame = 0

Query: 41  EELECPICWESFNIVENVPYVLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCH 100
           E LECPIC ESF   +  P +L CGH++C+ C+  L  S +            + CP+C 
Sbjct: 16  EVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSIN----------GVRCPFCS 68

Query: 101 QLS 104
           +++
Sbjct: 76  KIT 68

BLAST of Cucsat.G10259 vs. ExPASy Swiss-Prot
Match: Q8CH72 (E3 ubiquitin-protein ligase TRIM32 OS=Mus musculus OX=10090 GN=Trim32 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 2.7e-04
Identity = 22/63 (34.92%), Postives = 34/63 (53.97%), Query Frame = 0

Query: 41  EELECPICWESFNIVENVPYVLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCH 100
           E LECPIC ESF   +  P +L CGH++C+ C+  L  S +            + CP+C 
Sbjct: 17  EVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSIN----------GVRCPFCS 69

Query: 101 QLS 104
           +++
Sbjct: 77  KIT 69

BLAST of Cucsat.G10259 vs. NCBI nr
Match: XP_011655300.2 (uncharacterized protein LOC101218619 [Cucumis sativus] >XP_031741070.1 uncharacterized protein LOC101218619 [Cucumis sativus] >KAE8648500.1 hypothetical protein Csa_008920 [Cucumis sativus])

HSP 1 Score: 460 bits (1183), Expect = 4.77e-163
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY
Sbjct: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF
Sbjct: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSERRRLFFYNHLDFVVHLIFK 180
           LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSERRRLFFYNHLDFVVHLIFK
Sbjct: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSERRRLFFYNHLDFVVHLIFK 180

Query: 181 FLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHALEKLMNDITS 236
           FLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHALEKLMNDITS
Sbjct: 181 FLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHALEKLMNDITS 236

BLAST of Cucsat.G10259 vs. NCBI nr
Match: XP_008465708.1 (PREDICTED: uncharacterized protein LOC103503329 [Cucumis melo] >XP_008465709.1 PREDICTED: uncharacterized protein LOC103503329 [Cucumis melo] >XP_008465710.1 PREDICTED: uncharacterized protein LOC103503329 [Cucumis melo] >KAA0040509.1 zf-RING_LisH domain-containing protein [Cucumis melo var. makuwa] >TYK23453.1 zf-RING_LisH domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 424 bits (1091), Expect = 4.37e-149
Identity = 220/236 (93.22%), Postives = 225/236 (95.34%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNAIAGIRSKKNSDV KQVFWECSDDE CSEASRDEELECPICWESFNIVENVPY
Sbjct: 1   MWKLASNAIAGIRSKKNSDVPKQVFWECSDDEVCSEASRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGHSLCKNCVLGLQGSVLKLATRQ+RIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF
Sbjct: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQMRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSERRRLFFYNHLDFVVHLIFK 180
           LLWMVESFNGNEGKLDHSFNSDN  +FSLPSGT    VTSERRRLFFYNHLD V+HL+FK
Sbjct: 121 LLWMVESFNGNEGKLDHSFNSDNQSVFSLPSGT----VTSERRRLFFYNHLDCVIHLVFK 180

Query: 181 FLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHALEKLMNDITS 236
           FLL VIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLA HAL+KLMNDITS
Sbjct: 181 FLLTVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLALHALDKLMNDITS 232

BLAST of Cucsat.G10259 vs. NCBI nr
Match: XP_038890517.1 (uncharacterized protein LOC120080047 [Benincasa hispida])

HSP 1 Score: 417 bits (1071), Expect = 5.63e-146
Identity = 214/236 (90.68%), Postives = 220/236 (93.22%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNAIAGIR KKNS   KQVFWECSDDE CSEASRDEELECPICWESFN+VENVPY
Sbjct: 1   MWKLASNAIAGIRPKKNSTAPKQVFWECSDDEVCSEASRDEELECPICWESFNLVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPI +SCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIFLSCPWCHQLSLRVVYRGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSERRRLFFYNHLDFVVHLIFK 180
           LLWMVESFNGNEGK DHSFN DN P+FSLPS T T T  SERRRLFFYNHLDFVVHLIFK
Sbjct: 121 LLWMVESFNGNEGKHDHSFNGDNQPVFSLPSETDTDTDISERRRLFFYNHLDFVVHLIFK 180

Query: 181 FLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHALEKLMNDITS 236
           FLL+VIF+LIVVFVIPGSALILLLYLLITLL ALPSLLIFYLA HALEKLMNDITS
Sbjct: 181 FLLVVIFLLIVVFVIPGSALILLLYLLITLLLALPSLLIFYLALHALEKLMNDITS 236

BLAST of Cucsat.G10259 vs. NCBI nr
Match: KAG7029990.1 (hypothetical protein SDJN02_08334, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 383 bits (984), Expect = 1.61e-132
Identity = 203/249 (81.53%), Postives = 210/249 (84.34%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNA AGIRSK NS VSKQ FWECSDDE CSE SRDEELECPICWESFNIVENVPY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLKLATRQIRIPI ISCPWCHQLSLRVVYKGNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGT---------GTVT----SERRRLFF 180
           LLWMVES NGNEGKLDH FN DN P+FSLP              G V+      RRRLF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDANASQRHSFSDGRVSRHGIERRRRLFS 180

Query: 181 YNHLDFVVHLIFKFLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHAL 236
           Y HLD V+HLIFKFLL VIFVLIVVFVIPGSALILLLYLLIT LFALPSLLIFY++ HAL
Sbjct: 181 YEHLDCVIHLIFKFLLAVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

BLAST of Cucsat.G10259 vs. NCBI nr
Match: KAG6599045.1 (hypothetical protein SDJN03_08823, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 383 bits (983), Expect = 2.29e-132
Identity = 203/249 (81.53%), Postives = 210/249 (84.34%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNA AGIRSK NS VSKQ FWECSDDE CSE SRDEELECPICWESFNIVENVPY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLKLATRQIRIPI ISCPWCHQLSLRVVYKGNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGT---------GTVT----SERRRLFF 180
           LLWMVES NGNEGKLDH FN DN P+FSLP              G V+      RRRLF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDADASQRHSFSDGRVSRHGIERRRRLFS 180

Query: 181 YNHLDFVVHLIFKFLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHAL 236
           Y HLD V+HLIFKFLL VIFVLIVVFVIPGSALILLLYLLIT LFALPSLLIFY++ HAL
Sbjct: 181 YEHLDCVIHLIFKFLLAVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

BLAST of Cucsat.G10259 vs. ExPASy TrEMBL
Match: A0A1S3CPH8 (uncharacterized protein LOC103503329 OS=Cucumis melo OX=3656 GN=LOC103503329 PE=4 SV=1)

HSP 1 Score: 424 bits (1091), Expect = 2.11e-149
Identity = 220/236 (93.22%), Postives = 225/236 (95.34%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNAIAGIRSKKNSDV KQVFWECSDDE CSEASRDEELECPICWESFNIVENVPY
Sbjct: 1   MWKLASNAIAGIRSKKNSDVPKQVFWECSDDEVCSEASRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGHSLCKNCVLGLQGSVLKLATRQ+RIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF
Sbjct: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQMRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSERRRLFFYNHLDFVVHLIFK 180
           LLWMVESFNGNEGKLDHSFNSDN  +FSLPSGT    VTSERRRLFFYNHLD V+HL+FK
Sbjct: 121 LLWMVESFNGNEGKLDHSFNSDNQSVFSLPSGT----VTSERRRLFFYNHLDCVIHLVFK 180

Query: 181 FLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHALEKLMNDITS 236
           FLL VIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLA HAL+KLMNDITS
Sbjct: 181 FLLTVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLALHALDKLMNDITS 232

BLAST of Cucsat.G10259 vs. ExPASy TrEMBL
Match: A0A5A7TGX0 (Zf-RING_LisH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00120 PE=4 SV=1)

HSP 1 Score: 424 bits (1091), Expect = 2.11e-149
Identity = 220/236 (93.22%), Postives = 225/236 (95.34%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNAIAGIRSKKNSDV KQVFWECSDDE CSEASRDEELECPICWESFNIVENVPY
Sbjct: 1   MWKLASNAIAGIRSKKNSDVPKQVFWECSDDEVCSEASRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGHSLCKNCVLGLQGSVLKLATRQ+RIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF
Sbjct: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQMRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSERRRLFFYNHLDFVVHLIFK 180
           LLWMVESFNGNEGKLDHSFNSDN  +FSLPSGT    VTSERRRLFFYNHLD V+HL+FK
Sbjct: 121 LLWMVESFNGNEGKLDHSFNSDNQSVFSLPSGT----VTSERRRLFFYNHLDCVIHLVFK 180

Query: 181 FLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHALEKLMNDITS 236
           FLL VIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLA HAL+KLMNDITS
Sbjct: 181 FLLTVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLALHALDKLMNDITS 232

BLAST of Cucsat.G10259 vs. ExPASy TrEMBL
Match: A0A6J1G3L5 (uncharacterized protein LOC111450453 OS=Cucurbita moschata OX=3662 GN=LOC111450453 PE=4 SV=1)

HSP 1 Score: 381 bits (979), Expect = 4.50e-132
Identity = 202/249 (81.12%), Postives = 210/249 (84.34%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNA AGIRSK NS VSKQ FWECSDDE CSE SRDEELECPICWESFNIVENVPY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLKLATRQIRIPI ISCPWCHQLSLRVVYKGNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGT---------GTVT----SERRRLFF 180
           LLWMVES NGNEGKLDH FN DN P+FSLP              G V+      RRRLF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDADASQRHSFSDGRVSRHGIERRRRLFS 180

Query: 181 YNHLDFVVHLIFKFLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHAL 236
           Y HLD V+HLIFKFLL VIFVLIVVFVIPGSALILLLYLLIT LFALPSLLIFY++ HAL
Sbjct: 181 YEHLDCVIHLIFKFLLAVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

BLAST of Cucsat.G10259 vs. ExPASy TrEMBL
Match: A0A6J1KAL8 (uncharacterized protein LOC111493743 OS=Cucurbita maxima OX=3661 GN=LOC111493743 PE=4 SV=1)

HSP 1 Score: 377 bits (967), Expect = 3.03e-130
Identity = 202/249 (81.12%), Postives = 210/249 (84.34%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNA AGIRSK NS VSKQ FWECSDDE CSE SRDEELECPICWESFNIVENVPY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLKLATRQIRIPI ISCPWCHQLSLRVVYKGNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGT---------GTVTS---ERRR-LFF 180
           LLWMVES NGNEGKLDH FN DN P+FSLP              G V     ERR  LF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDADASQRHSFSDGRVNRHGIERRPWLFS 180

Query: 181 YNHLDFVVHLIFKFLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHAL 236
           Y HLD ++HLIFKFLL+VIFVLIVVFVIPGSALILLLYLLIT LFALPSLLIFY++ HAL
Sbjct: 181 YEHLDCLIHLIFKFLLVVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

BLAST of Cucsat.G10259 vs. ExPASy TrEMBL
Match: A0A6J1D940 (uncharacterized protein LOC111018502 OS=Momordica charantia OX=3673 GN=LOC111018502 PE=4 SV=1)

HSP 1 Score: 376 bits (966), Expect = 4.15e-130
Identity = 198/250 (79.20%), Postives = 217/250 (86.80%), Query Frame = 0

Query: 1   MWKLASNAIAGIRSKKNSDVSKQVFWECSDDEACSEASRDEELECPICWESFNIVENVPY 60
           MWKLASNAIAGIR +KNS VSKQVFWECSDDE CSEASRDE LECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAIAGIRLRKNSVVSKQVFWECSDDEVCSEASRDEGLECPICWESFNIVENIPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQIRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120
           VLWCGH+LCKNCVLGLQGSVLK+ATRQIR+PI ISCPWCHQLSLR+VYKGNLKFPSKNFF
Sbjct: 61  VLWCGHTLCKNCVLGLQGSVLKVATRQIRVPICISCPWCHQLSLRLVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNSDNHPLFSLPSGTGTGTVTSER--------------RRLF 180
           LLWMVESFN +EGKLDHSFN DN P+F LPS T T  V+SER              R LF
Sbjct: 121 LLWMVESFNDDEGKLDHSFNGDNQPVFPLPSDTET--VSSERLDSHVSRHGIGRRRRWLF 180

Query: 181 FYNHLDFVVHLIFKFLLLVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLAFHA 236
            Y +LD+++HLI KFLL+VIF+LIVVFVIPGSA+ILLLYLLIT LFALPSLL+FYLA HA
Sbjct: 181 VYKYLDWLIHLISKFLLVVIFLLIVVFVIPGSAVILLLYLLITFLFALPSLLVFYLALHA 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9H0X62.4e-0539.13RING finger protein 208 OS=Homo sapiens OX=9606 GN=RNF208 PE=1 SV=2[more]
Q8K0W39.1e-0539.34RING finger protein 208 OS=Mus musculus OX=10090 GN=Rnf208 PE=1 SV=2[more]
Q96D592.7e-0436.36E3 ubiquitin-protein ligase RNF183 OS=Homo sapiens OX=9606 GN=RNF183 PE=1 SV=2[more]
Q130492.7e-0434.92E3 ubiquitin-protein ligase TRIM32 OS=Homo sapiens OX=9606 GN=TRIM32 PE=1 SV=2[more]
Q8CH722.7e-0434.92E3 ubiquitin-protein ligase TRIM32 OS=Mus musculus OX=10090 GN=Trim32 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_011655300.24.77e-163100.00uncharacterized protein LOC101218619 [Cucumis sativus] >XP_031741070.1 uncharact... [more]
XP_008465708.14.37e-14993.22PREDICTED: uncharacterized protein LOC103503329 [Cucumis melo] >XP_008465709.1 P... [more]
XP_038890517.15.63e-14690.68uncharacterized protein LOC120080047 [Benincasa hispida][more]
KAG7029990.11.61e-13281.53hypothetical protein SDJN02_08334, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6599045.12.29e-13281.53hypothetical protein SDJN03_08823, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A1S3CPH82.11e-14993.22uncharacterized protein LOC103503329 OS=Cucumis melo OX=3656 GN=LOC103503329 PE=... [more]
A0A5A7TGX02.11e-14993.22Zf-RING_LisH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1G3L54.50e-13281.12uncharacterized protein LOC111450453 OS=Cucurbita moschata OX=3662 GN=LOC1114504... [more]
A0A6J1KAL83.03e-13081.12uncharacterized protein LOC111493743 OS=Cucurbita maxima OX=3661 GN=LOC111493743... [more]
A0A6J1D9404.15e-13079.20uncharacterized protein LOC111018502 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 33..137
e-value: 7.0E-13
score: 49.9
IPR027370RING-type zinc-finger, LisH dimerisation motifPFAMPF13445zf-RING_UBOXcoord: 45..73
e-value: 6.2E-8
score: 32.5
NoneNo IPR availablePANTHERPTHR46616:SF1TRANSCRIPTION FACTOR C2H2 FAMILY-RELATEDcoord: 1..149
NoneNo IPR availablePANTHERPTHR46616UBIQUITIN-PROTEIN LIGASEcoord: 1..149
NoneNo IPR availablePANTHERPTHR46616:SF1TRANSCRIPTION FACTOR C2H2 FAMILY-RELATEDcoord: 155..236
NoneNo IPR availablePANTHERPTHR46616UBIQUITIN-PROTEIN LIGASEcoord: 155..236
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 40..102
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 64..73
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 45..100
score: 9.951762

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G10259.T1Cucsat.G10259.T1mRNA
Cucsat.G10259.T2Cucsat.G10259.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity