Cucsat.G10257 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G10257
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Mannan endo-1,4-beta-mannosidase
Location	ctg1674: 7411 .. 11892 (-)
RNA-Seq Expression	Cucsat.G10257
Synteny	Cucsat.G10257

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTCATCCACCATGGGTGAAACTAAGATCCAAGCCATATCTCAATCCTTCCTCCATATCCTCACTTAGGCTCAGGTTCTTGGATCTCCGATCACGACCTTTTTCAGCCCTAGAACCCCCTGATAACCATAGATGGCACTACAGAAACACATTTATGTAGCCTACGAGTAAAAATCACCTTGGTACATTGGTATTGTGGTTTAAAAAACCAACCTTCACACTCCATTATCATAAACAACCATCTATAATTATGTTTCATATTTTTTCATATCAAATGTTTGAAAGTAACCACACACAGAAAAAAAAAAAGAACTCGTTCTCACTTTTCCCACTTCAGTTTGGCTTGAGACACAAATTTAGACCTCTCAGTTACCTTCAGATAGAAGGACGTCAAGAGAATGCACATTACATAAGTCTTAACGCGTAATTAACTATACTTTTAAGTGTTTTTCATGTAGGTGACTTAAAGAAGAAATGAAGTGAGGATCGCGCATTAACTCAACGAGAAGAAGTCTTGAAATTCATGAGACACAAGGTGAGATGCTAGTGCAAACTAGGAAAGATGATTCGTTGCCATCAATTTCTGAATGGTAACTAATATTATCAAACCGTAGGTACAATACTGTGTGGAGGGTGATGTGGAGAGGCTTTGCTCATCGGCGTGCACATCCTCCTCTGGTGGATCGAGCGGCGCATCAATAGATGGAGACAAGGCTGCAACGCAACACCAAAGGACGTAGTGGAGATTTGGGATGCGGTTTGTAGCTGTATTGTAGCATGAGGAAGCAACAACGGGTCTGGCAGCCGACTGACGAAAGGGAACAAGGTGGAGGAGAGGGAACAGTCCAAAGGAAGGGGAGGGGGCACGGTCGAGACCTCTTGAAGACGTTCTCGTGTTATGTTTCTTTTAATTGCTTCAAATAAAAAATACTAATAATAGATATAGACAAAAATACCCCCACTTTCAAAATAAACTCACTTTTTGCCCCTACCTCGCTCTTTTTAACACCTCAAAAAGTCCAAGCCTTAAACCAAACTAAATTTGTTCCAAACTTTCTTATTACAATCAATTTACCCTATTAAAACAATTCAACCTTAAAAGATTGACTATTGGATTTAAACTTGTTTTTCAAACTTAAAATGTTAATAAATAAAAGAAGAATGGAATGAGGGATCACAATCATCACTTGAGCGGAATGTTTAGTTAATCCCAGAGGACAATAATGTCTTAAATGAGGTATGAGATTTGAGTCCATTGACTACTAACGTGTGTCCTTTAGAGCTGGAAGACGATATAGATGGATTTGTTAGTGTTGTAAATGTAGAGTCATCTAACAATGACGACACATATGGCATGGAGATCATGTCATCATATTATGATGTTGAGGCGTATGAAGTATCTGAGTCGAATGTTAGTACTCATATATACTTCAATCATATTGAGCACTTGATAGACAATCATATACATATGGCAACACAGATTGAATGTACATTTTTAGAGTATAATGAAGTGAGAAAAGAGGACATGTGGGTGATATAAACCCAGCTCGTGAATGATCACTCAGACTGTCTAGTGGTCAGTGACGATGAACAACCGGTTAGTGATAAGTGAAAGCGTATTAAAAAATTGATGGATCACACTGTTGCTGTCTCAGCTAAGTTAACCTTGAAGATTGATAACTTGTAGAAATACAGGTTATTGCAGCCTTTCAGGTAAAACAATGGGTCAAAACACAAGATAAAGTGACAAAATGCATAACCTTCAGTGCAAAATTGTAACGTCAAGAGAATGCACATTACATAAGTCTTAACGCCTGATTATCTATGCTTTTAAGTGTTTTTCATGTAGGTGACTTAAAGAAGAAATGAAGTGAGGATCGCGCATTAACTCAACGAGAAGAAGACTTGAAATTCACGAGTCACAAGGTGAGATGCTAGTACAAAACTAGGAAAGATGATTTGTTGCCATCAATTTCTGAATGGTAACTAACATTATCAAACCGTAGGTACAAACTAGCACTAAGAAAACATACCAAAACTTACTTTTGCAACCTAATCATTACCAAGTCAAATATTAATTAATATAAACATCAAGTCAGCTAATATATTTCTTTTAATGAACTATAATCCTTAATTTAGACTTTAAAATAAATTTCAGTTACAACATTTCATCCACAGCTTCTTTGTTACAACTTCTTATCCTAGCTTGCTATTTCAATTTTTAATAGACCCTGTGGTATCATTCCTTCCGCTATATAAATATTGAAGAAGTGCATCTTTTGTCATATTGTGCTAACTTGAGAGCATTCATTCATTTTTTGAAATCAAGGACAAAATGACAGGACAACCGTGGAAAAATCTCGCTTTAGCTTGTGTTTTTCGTGTTGTTAACTTTTAAGGCCTATTCATTGCCTCTCTCGACAAATGGAAGATGGATTGTTGAGGCAACAACCGGCCAACGTGTGAAGTTGATGTGTGTAAATTGGCCGGGACACATGCAAGCCATGGTGGCAGAGGGTCTTCATCTCAGACCGCTTGACGACATTGCAACCATGGTGGCGAACTTGCGGTTTAATTGTGTGCGTTTGACATATTCGATTCACATGTTCACACGCTATGCTAATTTGACTGTGAAGCAATCCTTTGAGAATTTTGATATGAAAGAAGCCATAGCAGGTATAGCCCAAAACAATCCTACTATATTGAACATGAAGGTCGTTGAGGCTTATGAAGCAGTAGTTGATTCACTTGGTGCACATGGAGTTATGGTAGTTTCTGATAATCATATAAGCCAACCAAGATGGTGTTGTAGCAATGACGATGGCAATGGCTTTTTTGGAGATCGCTATTTCAATTCTCAAGAATGGCTAGAAGGACTTAGTTTGGCAGCTCAAAGCCTAAAAAACAAACCTCAGGTCTGGTTTATTTATTTACTTTGTTTTCATTAAACTACTTCTAAAAGAACTATAAAATAGGCTGAAGCATATAATAAAAAGACAACAGAAAAAGCCTTAATTTAACACATTTTAGACAATAAATTAACTACATCTAAATTATTAAGGAAGATACAAAAAAAAACAATTTAATCCCTCCAATTCATATATAACACAACTCTTAAAATAGAGGGGCAGTATTTGTACTTCAACCTATAAGTTATCATTAATTGTTTTATATGACGTTTTGACAAATTAGATGATAATTTATATGTAAATTTAAAGGTGGTGGCAATGAGTTTGAGAAACGAACCACGAGGACCAAATCAAAATGTGGAGATGTGGTTTCAATATATGAGCCAAGGAGCTAAACTTGTTCACCAAATTAACCCAAACGCTTTAGTAGTGGTTTCTGGACTAAGTTATGACACCGATCTAAGCTTCTTGAAGAATAGGTCAATGGGCTTCAATTTGGACAACAAGCTTGTATTTGAAGCTCACTTGTACTCCTTCACAAACAACATGAGTGATTATTGGACGTCAAAGCCATTGAACACGTTTTGTGCGAATGTCAATCAAGGATTTGAAGACCGTGCTGGATTTCTTGTGAGAGGACAAAACCCAATACCTCTCTTTGTGAGTGAGTTTGGGATCAACCAAATGGGAGCAAATGAAGGACAAAATCGATTCTTGAGTTGCTTTTTTACCTATCTTACTGAGAATGATTTTGATTGGGGCCTATGGGCATTGCAAGGTAGCTACTACTATAGAGAAGGTGTGAAAAACGATGAAGAAACCTTTGGCGTCTTAGATGCCAAGTTTACAAATGTCAAAAACCCGAAATTCCTTCAGAAGTTTCAGCTTATGCAAACCAAGCTTCAAGGTATATAACGTTTAAAAGTGATACATACATTTGAATCATGTTTTAGTTAAGAATAATGTTAGTCTCTATTCATTTTTTCTTCTCACCGATGAATCACACAATTGCTCTTTAACACAGATCCAATCTCAAATCTCACAACCTCATTCATAATGTACCATCCTCTTAGTGGTGAGTGCGTGCGAATGAACAAAAAGTACCAACTAGGAGTTAGTAGCTGCAAGACATCAAATCGTTGGAGTCACGAACAAGATGGCACTCCAATTAAGTTGGCAGGATCTATATTGTGTTTGCAAGCTGTAGGAGATGGACTCCCTCCAATTCTATCTAAAGACTGCTCAAGCCAACAAAGTGCATGGAAATATGCTTCAAATGCTAAGCTTCAACTGGCCACTGTTGATGAAGAAGGACAAGCCTTGTGTTTGCAAAGGGCTTCTCATTCCCATCAAATTTTGACTAACAAATGCATGTGTCCTAATGATTCTGAATGCCAAGGAGATCCACAAAGTCAATGGTTTACACTTGTACCATCAAATGTACATCTCAATTAATTTATGTACAAAATTGTAATTATCTATCTATTTGTTACACATCCTCCATTTTAATTTGAACGAGTTTATTATTCAAACATTTTTCATGAAGGCCAAATAAGAAGTTTAATTAGATGTTT

Coding sequence (CDS)

ATGTGTGTAAATTGGCCGGGACACATGCAAGCCATGGTGGCAGAGGGTCTTCATCTCAGACCGCTTGACGACATTGCAACCATGGTGGCGAACTTGCGGTTTAATTGTGTGCGTTTGACATATTCGATTCACATGTTCACACGCTATGCTAATTTGACTGTGAAGCAATCCTTTGAGAATTTTGATATGAAAGAAGCCATAGCAGGTATAGCCCAAAACAATCCTACTATATTGAACATGAAGGTCGTTGAGGCTTATGAAGCAGTAGTTGATTCACTTGGTGCACATGGAGTTATGGTAGTTTCTGATAATCATATAAGCCAACCAAGATGGTGTTGTAGCAATGACGATGGCAATGGCTTTTTTGGAGATCGCTATTTCAATTCTCAAGAATGGCTAGAAGGACTTAGTTTGGCAGCTCAAAGCCTAAAAAACAAACCTCAGGTGGTGGCAATGAGTTTGAGAAACGAACCACGAGGACCAAATCAAAATGTGGAGATGTGGTTTCAATATATGAGCCAAGGAGCTAAACTTGTTCACCAAATTAACCCAAACGCTTTAGTAGTGGTTTCTGGACTAAGTTATGACACCGATCTAAGCTTCTTGAAGAATAGGTCAATGGGCTTCAATTTGGACAACAAGCTTGTATTTGAAGCTCACTTGTACTCCTTCACAAACAACATGAGTGATTATTGGACGTCAAAGCCATTGAACACGTTTTGTGCGAATGTCAATCAAGGATTTGAAGACCGTGCTGGATTTCTTGTGAGAGGACAAAACCCAATACCTCTCTTTGTGAGTGAGTTTGGGATCAACCAAATGGGAGCAAATGAAGGACAAAATCGATTCTTGAGTTGCTTTTTTACCTATCTTACTGAGAATGATTTTGATTGGGGCCTATGGGCATTGCAAGGTAGCTACTACTATAGAGAAGGTGTGAAAAACGATGAAGAAACCTTTGGCGTCTTAGATGCCAAGTTTACAAATGTCAAAAACCCGAAATTCCTTCAGAAGTTTCAGCTTATGCAAACCAAGCTTCAAGATCCAATCTCAAATCTCACAACCTCATTCATAATGTACCATCCTCTTAGTGGTGAGTGCGTGCGAATGAACAAAAAGTACCAACTAGGAGTTAGTAGCTGCAAGACATCAAATCGTTGGAGTCACGAACAAGATGGCACTCCAATTAAGTTGGCAGGATCTATATTGTGTTTGCAAGCTGTAGGAGATGGACTCCCTCCAATTCTATCTAAAGACTGCTCAAGCCAACAAAGTGCATGGAAATATGCTTCAAATGCTAAGCTTCAACTGGCCACTGTTGATGAAGAAGGACAAGCCTTGTGTTTGCAAAGGGCTTCTCATTCCCATCAAATTTTGACTAACAAATGCATGTGTCCTAATGATTCTGAATGCCAAGGAGATCCACAAAGTCAATGGTTTACACTTGTACCATCAAATGTACATCTCAATTAA

Protein sequence

MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFENFDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVHQINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNDEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPISNLTTSFIMYHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKDCSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWFTLVPSNVHLN

Homology

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BLAST of Cucsat.G10257 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 3.2e-113
Identity = 217/505 (42.97%), Postives = 302/505 (59.80%), Query Frame = 0


Query: 2   CVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTR--YANLTVKQSFE 61
           CVNW GH++  + EGL+  P+  +A  +++L FNCVRLTYSIHM TR  Y N TV Q+F 
Sbjct: 51  CVNWVGHLEPGLPEGLNRLPVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFA 110

Query: 62  NFDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGN 121
             ++ EA +GI  NNP +L++  V AY  VV +L   GVMV+ DNH+S+P+WCC+ DDGN
Sbjct: 111 RLNLTEAASGIEHNNPELLDLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGN 170

Query: 122 GFFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLV 181
           GFFGDRYFN   W+EGL L A    N P VVAMSLRNE RG       W ++M  GA  V
Sbjct: 171 GFFGDRYFNPNTWVEGLGLMATYFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATV 230

Query: 182 HQINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNT 241
           H+ NP  LV++SGL +DTDLSFL    +      K+V+E H YSF       W +   N 
Sbjct: 231 HKANPKVLVILSGLQFDTDLSFLPVLPVTLPFKEKIVYEGHWYSF----GVPWRTGLPND 290

Query: 242 FCANVNQGFEDRAGFLVRGQN--PIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFD 301
            C N    F+   GF+    N    PLF+SEFGI+Q   N+  NR+L+C   YL E D D
Sbjct: 291 VCKNETGRFKSNVGFVTSSANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLD 350

Query: 302 WGLWALQGSYYYR---EGVKNDEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPISNLT 361
           W LW + GSYYYR   + VK+ EET+G  +  ++ ++NP F+ + + +Q  +QDP     
Sbjct: 351 WALWTMGGSYYYRSDKQPVKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPG 410

Query: 362 TSF-IMYHPLSGECVRMNKKYQLGVSSCKT-SNRWSHEQD-GTPIKLAGSILCLQAVGDG 421
             + I+YHP SG CV       + + SC++  +RW+++     PI L GS  C+   G+G
Sbjct: 411 PYYQIIYHPASGLCVESGIGNTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNG 470

Query: 422 LPPILSKDCSS-QQSAWKYASNAKLQLAT----VDEEGQALCLQRASHSHQILTNKCMCP 481
           LP I++++CS+   + W   S+A+LQL T     D + + +CL   S S  I TN+C+C 
Sbjct: 471 LPAIMTENCSAPNNTLWSTVSSAQLQLGTRVLGKDGKEKWMCLD-GSKSPLISTNECICI 530

Query: 482 NDSEC--QGDPQSQWFTLVPSNVHL 490
            DS C  + +P+ QWF ++ +N  L
Sbjct: 531 TDSHCYPKLNPEKQWFKVITTNKQL 550

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BLAST of Cucsat.G10257 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 3.7e-16
Identity = 76/354 (21.47%), Postives = 145/354 (40.96%), Query Frame = 0


Query: 3   VNWPG-HMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFENF 62
           +NW G         GL  R +DD+   V    +N +RL YS             Q F++ 
Sbjct: 59  LNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYS------------NQLFDSS 118

Query: 63  DMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGF 122
              ++I      NP ++ +  ++  + +++  G  G+ ++ D H            G+G 
Sbjct: 119 SRPDSID--YHKNPDLVGLNPIQIMDKLIEKAGQRGIQIILDRH----------RPGSGG 178

Query: 123 FGDRYFNSQ----EWLEGLSLAAQSLKNKPQVVAMSLRNEPRGP------NQNVEMWFQY 182
             + ++ SQ     W+    + A   KN P V+   L NEP G       N + + W   
Sbjct: 179 QSELWYTSQYPESRWISDWKMLADRYKNNPTVIGADLHNEPHGQASWGTGNASTD-WRLA 238

Query: 183 MSQGAKLVHQINPNALVVVSGLSYDT-----------DLSFLKNRSMGFNLDNKLVFEAH 242
             +    +  +NPN L++V G+ ++            +L+ + N  +  ++ N++V+  H
Sbjct: 239 AQRAGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLTGVANYPVVLDVPNRVVYSPH 298

Query: 243 LYSFTNNMSDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQ 302
            Y      S  W + P   F +N+   ++   G++ + QN  P+ V EFG   +  +  +
Sbjct: 299 DYG-PGVSSQPWFNDP--AFPSNLPAIWDQTWGYISK-QNIAPVLVGEFGGRNVDLSCPE 358

Query: 303 NRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNDEETFGVLDAKFTNVKNPK 335
            ++ +    Y+  N+  +  W+L           N  +T G+L   +T    PK
Sbjct: 359 GKWQNALVHYIGANNLYFTYWSLN---------PNSGDTGGLLLDDWTTWNRPK 374

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BLAST of Cucsat.G10257 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 1.7e-05
Identity = 70/341 (20.53%), Postives = 121/341 (35.48%), Query Frame = 0


Query: 13  VAEGLHLRPLDDIATMVANLRFNCVRLTY---SIHMFTRYANLTVKQSFENFDMKEAIAG 72
           V  GL  R   D+   +  L FN VRL +   ++   T  A++   +             
Sbjct: 58  VMHGLWARNWKDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSR------------- 117

Query: 73  IAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNS 132
               N  +  +  ++  + V+    A G+ V+ D+H       C+      + G   +  
Sbjct: 118 ----NADLQGLTSLQILDKVIAEFNARGMYVLLDHHTPD----CAGISELWYTGS--YTE 177

Query: 133 QEWLEGLSLAAQSLKNKPQVVAMSLRNEPR-----GPNQNVEMWFQYMSQGAKLVHQINP 192
            +WL  L   A   KN P V+ + L+NEP      G       W +   +G+  V  + P
Sbjct: 178 AQWLADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAP 237

Query: 193 NALVVVSGLSYDTDLSFLKNRSMGFNLD-----------NKLVFEAHLYSFTNNMSDYWT 252
             L+ V G++ +   S       G NL            N+L+   H+Y     +  Y+ 
Sbjct: 238 KWLIAVEGITDNPVCSTNGGIFWGGNLQPLACTPLNIPANRLLLAPHVYGPDVFVQSYFN 297

Query: 253 SKPLNTFCANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTE 312
               + F  N+   +E   G   +      L + EFG      +     +      YL  
Sbjct: 298 D---SNFPNNMPAIWERHFG---QFAGTHALLLGEFGGKYGEGDARDKTWQDALVKYLRS 357

Query: 313 NDFDWGLWALQGSYYYREGVKNDEETFGVLDAKFTNVKNPK 335
              + G        +Y     N  +T G+L   +T+V+  K
Sbjct: 358 KGINQG--------FYWSWNPNSGDTGGILRDDWTSVRQDK 361

[Close]

BLAST of Cucsat.G10257 vs. NCBI nr
Match: KAE8648503.1 (hypothetical protein Csa_009407 [Cucumis sativus])

HSP 1 Score: 1012 bits (2617), Expect = 0.0
Identity = 486/490 (99.18%), Postives = 488/490 (99.59%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN
Sbjct: 46  MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 105

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG
Sbjct: 106 FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 165

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH
Sbjct: 166 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 225

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF
Sbjct: 226 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 285

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL
Sbjct: 286 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 345

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPISNLTTSFIMY 360
           WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDP SNLTTSFIMY
Sbjct: 346 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMY 405

Query: 361 HPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKDC 420
           HPLSGECV++NKKYQLGVSSCKTSNRWSHEQDGTPIKL GSILCLQAVGDGLPPILSKDC
Sbjct: 406 HPLSGECVQVNKKYQLGVSSCKTSNRWSHEQDGTPIKLVGSILCLQAVGDGLPPILSKDC 465

Query: 421 SSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF 480
           SSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF
Sbjct: 466 SSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF 525

Query: 481 TLVPSNVHLN 490
           TLVPSNVHLN
Sbjct: 526 TLVPSNVHLN 535

[Close]

BLAST of Cucsat.G10257 vs. NCBI nr
Match: KAE8648505.1 (hypothetical protein Csa_007897 [Cucumis sativus])

HSP 1 Score: 1006 bits (2600), Expect = 0.0
Identity = 483/487 (99.18%), Postives = 485/487 (99.59%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN
Sbjct: 46  MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 105

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG
Sbjct: 106 FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 165

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH
Sbjct: 166 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 225

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF
Sbjct: 226 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 285

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL
Sbjct: 286 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 345

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPISNLTTSFIMY 360
           WALQGSYYYREGVKNDEETFGVLD+KFTNVKNPKFLQKFQLMQTKLQDP SNLTTSFIMY
Sbjct: 346 WALQGSYYYREGVKNDEETFGVLDSKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMY 405

Query: 361 HPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKDC 420
           HPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKL GSILCLQAVGDGLPPILSKDC
Sbjct: 406 HPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLVGSILCLQAVGDGLPPILSKDC 465

Query: 421 SSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF 480
           SSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF
Sbjct: 466 SSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF 525

Query: 481 TLVPSNV 487
           TLVPSN+
Sbjct: 526 TLVPSNL 532

[Close]

BLAST of Cucsat.G10257 vs. NCBI nr
Match: XP_031739082.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8650476.1 hypothetical protein Csa_010408 [Cucumis sativus])

HSP 1 Score: 988 bits (2553), Expect = 0.0
Identity = 471/490 (96.12%), Postives = 479/490 (97.76%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           +CVNWPGHMQAMVAEGLHL+PLDDIA MV  LRFNCVRLTYSIHMFTRYANLTVKQSFEN
Sbjct: 46  ICVNWPGHMQAMVAEGLHLKPLDDIAAMVVKLRFNCVRLTYSIHMFTRYANLTVKQSFEN 105

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FD+KEAI GIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG
Sbjct: 106 FDLKEAIVGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 165

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYFNSQEWL+GLSLA QSLK KPQVVAMSLRNEPRGPNQNVEMWFQYMSQG KLVH
Sbjct: 166 FFGDRYFNSQEWLQGLSLATQSLKTKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGTKLVH 225

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM DYWTSKPLNTF
Sbjct: 226 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTF 285

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMG NEGQNRFLSCFFTYLT+NDFDWGL
Sbjct: 286 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGVNEGQNRFLSCFFTYLTKNDFDWGL 345

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPISNLTTSFIMY 360
           WALQGSYYYREGVKNDEETFGVLD+KFTNVKNPKFLQKFQLMQTKLQDP SNLTTSFIMY
Sbjct: 346 WALQGSYYYREGVKNDEETFGVLDSKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMY 405

Query: 361 HPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKDC 420
           HPLSGECVRMNKKYQLGVSSCKTSNRWSHEQD TPIKLAGSILCLQAVGDGLPPILSKDC
Sbjct: 406 HPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDDTPIKLAGSILCLQAVGDGLPPILSKDC 465

Query: 421 SSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF 480
           SSQQSAWKYASNAKLQLATVDE+GQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF
Sbjct: 466 SSQQSAWKYASNAKLQLATVDEQGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF 525

Query: 481 TLVPSNVHLN 490
           TLVPSNVHL+
Sbjct: 526 TLVPSNVHLS 535

[Close]

BLAST of Cucsat.G10257 vs. NCBI nr
Match: KAA0042984.1 (endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 890 bits (2301), Expect = 0.0
Identity = 419/490 (85.51%), Postives = 453/490 (92.45%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNW GHMQ M+ EGLH RPLDDIA +VA LRFNCVRLTYSIHMFTR+AN+TVKQSFEN
Sbjct: 48  MCVNWAGHMQGMLVEGLHRRPLDDIAALVAKLRFNCVRLTYSIHMFTRHANVTVKQSFEN 107

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FDMK+A+ GIAQNNP+ILN+ +VEAY AVVDSL AHG+MVVSDNHISQPRWCC NDDGNG
Sbjct: 108 FDMKDALVGIAQNNPSILNLTLVEAYGAVVDSLAAHGIMVVSDNHISQPRWCCDNDDGNG 167

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYF+ QEW +G+SLAAQSLK+K QVVAMSLRNEPRGPNQNVE WFQYMSQGAKL+H
Sbjct: 168 FFGDRYFDPQEWFQGISLAAQSLKSKAQVVAMSLRNEPRGPNQNVEKWFQYMSQGAKLIH 227

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFL+NRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTF
Sbjct: 228 QINPNALVVVSGLSYDTDLSFLRNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTF 287

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CA++NQGFEDRAGFLVRGQNPIPLFVSEFGI+Q G NEGQNRFLSCFF+YLTENDFDWGL
Sbjct: 288 CASINQGFEDRAGFLVRGQNPIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGL 347

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPK-FLQKFQLMQTKLQDPISNLTTSFIM 360
           WALQGSYYY+ GVKN EE FGVLD+ FT  KN K FLQ+FQLMQTKLQDP SN TTSFIM
Sbjct: 348 WALQGSYYYKVGVKNAEENFGVLDSNFTKAKNSKLFLQRFQLMQTKLQDPSSNFTTSFIM 407

Query: 361 YHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKD 420
           YHPLSG C+RMNKKYQLG+SSCKTSNRWSHEQDG PIKLAGSILCL+A+G GLPPILS+D
Sbjct: 408 YHPLSGGCMRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQD 467

Query: 421 CSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQW 480
           CSSQQS WKY SNAKLQLATVDE+GQALCLQRASHSHQI+TNKC+C NDS+CQ DPQSQW
Sbjct: 468 CSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSNDSQCQEDPQSQW 527

Query: 481 FTLVPSNVHL 489
           FTLVPSN+ L
Sbjct: 528 FTLVPSNLRL 537

[Close]

BLAST of Cucsat.G10257 vs. NCBI nr
Match: XP_038874277.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 418/490 (85.31%), Postives = 458/490 (93.47%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNWPGHMQ M+AEGLHLRPLDDIA +VA LRFNCVRLTYSIHMFTRYANLTV+QSFEN
Sbjct: 46  MCVNWPGHMQVMLAEGLHLRPLDDIAVLVAKLRFNCVRLTYSIHMFTRYANLTVQQSFEN 105

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FD+KEA+AGIA+NNP+ILNM ++EAY+AVVDSL  HGVMVVSDNHISQPRWCC+NDDGNG
Sbjct: 106 FDLKEAMAGIARNNPSILNMTLIEAYKAVVDSLAVHGVMVVSDNHISQPRWCCNNDDGNG 165

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYF+ QEWL+G+SLAAQSLK+K QVVAMSLRNEPRGPNQNVEMWFQYMSQGAKL+H
Sbjct: 166 FFGDRYFDPQEWLQGISLAAQSLKSKFQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLIH 225

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           Q NP+ALVV+SGLSYDTDLSFLKNRS+GFNL+NKLVFEAHLYSFTNNMSD WTSKPLNTF
Sbjct: 226 QNNPDALVVISGLSYDTDLSFLKNRSLGFNLNNKLVFEAHLYSFTNNMSDSWTSKPLNTF 285

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CANVNQGFEDRAGFLVRGQNP PLFVSEFGI+Q G N+GQNR+LSCFFTYL ENDFDWGL
Sbjct: 286 CANVNQGFEDRAGFLVRGQNPTPLFVSEFGIDQTGKNDGQNRYLSCFFTYLVENDFDWGL 345

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPISNLTTSFIMY 360
           WALQGSYYYREGVKN EE+FGV D  F + KNPKFLQ+FQLMQTKLQDP SNLT SFIMY
Sbjct: 346 WALQGSYYYREGVKNAEESFGVSDFNFRHAKNPKFLQRFQLMQTKLQDPSSNLTASFIMY 405

Query: 361 HPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKDC 420
           H LSGECVRM+KKYQL VSSCKTSNRW+H++DG PIKL  S+LCL+A+G+GLPPILSKDC
Sbjct: 406 HSLSGECVRMDKKYQLRVSSCKTSNRWNHKEDGAPIKLVDSLLCLKALGEGLPPILSKDC 465

Query: 421 SSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWF 480
           SSQQS WKYASNAKLQLATVD++GQALCLQ  SHSHQI+TNKCMC +DS+CQGDPQSQWF
Sbjct: 466 SSQQSVWKYASNAKLQLATVDKQGQALCLQSVSHSHQIVTNKCMCLDDSQCQGDPQSQWF 525

Query: 481 TLVPSNVHLN 490
           TLV SNV+LN
Sbjct: 526 TLVFSNVNLN 535

[Close]

BLAST of Cucsat.G10257 vs. ExPASy TrEMBL
Match: A0A5A7TI19 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00580 PE=3 SV=1)

HSP 1 Score: 890 bits (2301), Expect = 0.0
Identity = 419/490 (85.51%), Postives = 453/490 (92.45%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNW GHMQ M+ EGLH RPLDDIA +VA LRFNCVRLTYSIHMFTR+AN+TVKQSFEN
Sbjct: 48  MCVNWAGHMQGMLVEGLHRRPLDDIAALVAKLRFNCVRLTYSIHMFTRHANVTVKQSFEN 107

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FDMK+A+ GIAQNNP+ILN+ +VEAY AVVDSL AHG+MVVSDNHISQPRWCC NDDGNG
Sbjct: 108 FDMKDALVGIAQNNPSILNLTLVEAYGAVVDSLAAHGIMVVSDNHISQPRWCCDNDDGNG 167

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYF+ QEW +G+SLAAQSLK+K QVVAMSLRNEPRGPNQNVE WFQYMSQGAKL+H
Sbjct: 168 FFGDRYFDPQEWFQGISLAAQSLKSKAQVVAMSLRNEPRGPNQNVEKWFQYMSQGAKLIH 227

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFL+NRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTF
Sbjct: 228 QINPNALVVVSGLSYDTDLSFLRNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTF 287

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CA++NQGFEDRAGFLVRGQNPIPLFVSEFGI+Q G NEGQNRFLSCFF+YLTENDFDWGL
Sbjct: 288 CASINQGFEDRAGFLVRGQNPIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGL 347

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPK-FLQKFQLMQTKLQDPISNLTTSFIM 360
           WALQGSYYY+ GVKN EE FGVLD+ FT  KN K FLQ+FQLMQTKLQDP SN TTSFIM
Sbjct: 348 WALQGSYYYKVGVKNAEENFGVLDSNFTKAKNSKLFLQRFQLMQTKLQDPSSNFTTSFIM 407

Query: 361 YHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKD 420
           YHPLSG C+RMNKKYQLG+SSCKTSNRWSHEQDG PIKLAGSILCL+A+G GLPPILS+D
Sbjct: 408 YHPLSGGCMRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQD 467

Query: 421 CSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQW 480
           CSSQQS WKY SNAKLQLATVDE+GQALCLQRASHSHQI+TNKC+C NDS+CQ DPQSQW
Sbjct: 468 CSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSNDSQCQEDPQSQW 527

Query: 481 FTLVPSNVHL 489
           FTLVPSN+ L
Sbjct: 528 FTLVPSNLRL 537

[Close]

BLAST of Cucsat.G10257 vs. ExPASy TrEMBL
Match: A0A5A7UGS0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00740 PE=3 SV=1)

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 422/488 (86.48%), Postives = 454/488 (93.03%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNWPGHMQ M+AEGLHLRPL+DIA +VA LRFNCVRLTYSIHMFTR+ANLTV+QSFEN
Sbjct: 30  MCVNWPGHMQGMLAEGLHLRPLNDIAALVAKLRFNCVRLTYSIHMFTRHANLTVQQSFEN 89

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FD+K  IAGIAQNNP+ILNM +V+AY AVVDSL AH +MVVSDNHISQPRWCC N+DGNG
Sbjct: 90  FDLKNTIAGIAQNNPSILNMTLVQAYGAVVDSLAAHRIMVVSDNHISQPRWCCDNNDGNG 149

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYF+ QEWL+G+SLAAQSLK+K QVVAMSLRNE RGPNQNVE WFQYMSQGAKL+H
Sbjct: 150 FFGDRYFDPQEWLQGISLAAQSLKSKSQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIH 209

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTF
Sbjct: 210 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTF 269

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CA+VNQGFEDRAGFLVRGQ+PIPLFVSEFGI+Q G NEGQ RFL+CFFTYLTENDFDWGL
Sbjct: 270 CASVNQGFEDRAGFLVRGQSPIPLFVSEFGIDQTGVNEGQKRFLNCFFTYLTENDFDWGL 329

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPK-FLQKFQLMQTKLQDPISNLTTSFIM 360
           WALQGSYYYREGVKN EETFGVLD+ F   KN K FLQKFQLMQ+KLQDP SNLTTSFIM
Sbjct: 330 WALQGSYYYREGVKNAEETFGVLDSTFAKAKNSKLFLQKFQLMQSKLQDPSSNLTTSFIM 389

Query: 361 YHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKD 420
           YHPLSG CVRMNKKYQLG+SSCKTSNRWSHEQDG PIKLAGSILCL+A+G GLPPILS+D
Sbjct: 390 YHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQD 449

Query: 421 CSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQW 480
           CSSQQS WKY SNAKLQLATVDE+GQALCLQRASHSHQI+TNKC+C NDS+CQ DPQSQW
Sbjct: 450 CSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSNDSQCQEDPQSQW 509

Query: 481 FTLVPSNV 487
           FTLVPSN+
Sbjct: 510 FTLVPSNL 517

[Close]

BLAST of Cucsat.G10257 vs. ExPASy TrEMBL
Match: A0A5D3BML8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00620 PE=3 SV=1)

HSP 1 Score: 885 bits (2288), Expect = 0.0
Identity = 417/490 (85.10%), Postives = 454/490 (92.65%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNWPGHMQ M+AEGLHLRPL+DIA +V  LRFNCVRLTYSIHMFTR+ANLTV+QSFEN
Sbjct: 30  MCVNWPGHMQGMLAEGLHLRPLNDIAALVVKLRFNCVRLTYSIHMFTRHANLTVQQSFEN 89

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FDMK+ IAGIAQNNP+ILN+ +V+AYE V+DSL  HG+MVVSDNHISQPRWCC N+DGNG
Sbjct: 90  FDMKDTIAGIAQNNPSILNLTLVQAYETVIDSLAVHGIMVVSDNHISQPRWCCDNNDGNG 149

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYF+ QEWL+G+SLAAQSLK+K QVVAMSLRNE RGPNQNVE WFQYMSQGAKL+H
Sbjct: 150 FFGDRYFDPQEWLQGISLAAQSLKSKSQVVAMSLRNELRGPNQNVETWFQYMSQGAKLIH 209

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM D+WTSKPLNTF
Sbjct: 210 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWTSKPLNTF 269

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CA+VNQGFEDRAGFLVRG+NPIPLFVSEFGI+Q G NEGQNRFL+CFFTYLTENDFDWGL
Sbjct: 270 CASVNQGFEDRAGFLVRGKNPIPLFVSEFGIDQTGVNEGQNRFLNCFFTYLTENDFDWGL 329

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPK-FLQKFQLMQTKLQDPISNLTTSFIM 360
           WALQGSYYYREGVKN +E FGVLD+ F   KN K FL+KFQLMQ+KLQD  SNLTTSFIM
Sbjct: 330 WALQGSYYYREGVKNAKENFGVLDSTFAKAKNSKLFLKKFQLMQSKLQDSSSNLTTSFIM 389

Query: 361 YHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKD 420
           YHPLSG CVRMNKKYQLG+SSCKTSNRWSHEQDG PIKLAGSILCL+A+G GLPPILS+D
Sbjct: 390 YHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQD 449

Query: 421 CSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQW 480
           CSSQQS WKY S+AKLQLATVDE+GQ LCLQRASHSHQI+TNKC+C NDS+CQ DPQSQW
Sbjct: 450 CSSQQSIWKYGSDAKLQLATVDEQGQVLCLQRASHSHQIVTNKCLCSNDSQCQEDPQSQW 509

Query: 481 FTLVPSNVHL 489
           FTLVPSN+ L
Sbjct: 510 FTLVPSNLRL 519

[Close]

BLAST of Cucsat.G10257 vs. ExPASy TrEMBL
Match: A0A5A7UKF5 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G00930 PE=3 SV=1)

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 420/488 (86.07%), Postives = 452/488 (92.62%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNWPGHMQ M+AEGLHLRPL+DIA +VA LRFNCVRLTYSIHMFTR+ANLTV+QSFEN
Sbjct: 30  MCVNWPGHMQGMLAEGLHLRPLNDIAALVAKLRFNCVRLTYSIHMFTRHANLTVQQSFEN 89

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FD+K  IAGIAQNNP+ILNM +V+AY AVVDSL AH +MVVSDNHISQPRWCC N+DGNG
Sbjct: 90  FDLKNTIAGIAQNNPSILNMTLVQAYGAVVDSLAAHRIMVVSDNHISQPRWCCDNNDGNG 149

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYF+ QEWL+G+SLAAQSLK+K QVVAMSLRNE RGPNQNVE WFQYMSQGAKL+H
Sbjct: 150 FFGDRYFDPQEWLQGISLAAQSLKSKSQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIH 209

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTF
Sbjct: 210 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTF 269

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CA+VNQGFEDRAGFLVRGQ+PIPLFVSEFGI+Q G NEGQ RFL+CFFTYLTENDFDWGL
Sbjct: 270 CASVNQGFEDRAGFLVRGQSPIPLFVSEFGIDQTGVNEGQKRFLNCFFTYLTENDFDWGL 329

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPK-FLQKFQLMQTKLQDPISNLTTSFIM 360
           WALQGSYYYREGVKN EETFGVLD+ F   KN K FLQKFQLMQ+KLQDP SNL TSFIM
Sbjct: 330 WALQGSYYYREGVKNAEETFGVLDSTFAKAKNSKLFLQKFQLMQSKLQDPSSNLITSFIM 389

Query: 361 YHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKD 420
           YHPLSG CVRMNKKYQLG+SSCKTSNRWSHEQDG PIKL GSILCL+A+G GLPPILS+D
Sbjct: 390 YHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLTGSILCLKAIGVGLPPILSQD 449

Query: 421 CSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQW 480
           CSSQQS WKY SNAKLQLATVDE+GQALCLQRASHSHQI+TNKC+C NDS+CQ DPQSQW
Sbjct: 450 CSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSNDSQCQEDPQSQW 509

Query: 481 FTLVPSNV 487
           FTLVPSN+
Sbjct: 510 FTLVPSNL 517

[Close]

BLAST of Cucsat.G10257 vs. ExPASy TrEMBL
Match: A0A5A7UKP6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00780 PE=3 SV=1)

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 416/490 (84.90%), Postives = 453/490 (92.45%), Query Frame = 0


Query: 1   MCVNWPGHMQAMVAEGLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFEN 60
           MCVNWPGHM  M+AEGLHLRPL+DIA +V  LRFNCVRLTYSIHMFTR+ANLTV+QSFEN
Sbjct: 30  MCVNWPGHMHGMLAEGLHLRPLNDIAALVVKLRFNCVRLTYSIHMFTRHANLTVQQSFEN 89

Query: 61  FDMKEAIAGIAQNNPTILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNG 120
           FDMK+ IAGIAQNNP+ILN+ +V+AYE V+DSL  HG+MVVSDNHISQPRWCC N+DGNG
Sbjct: 90  FDMKDTIAGIAQNNPSILNLTLVQAYETVIDSLAVHGIMVVSDNHISQPRWCCDNNDGNG 149

Query: 121 FFGDRYFNSQEWLEGLSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVH 180
           FFGDRYF+ QEWL+G+SLAAQSLK+K QVVAMSLRNE RGPNQNVE WFQYMSQGAKL+H
Sbjct: 150 FFGDRYFDPQEWLQGISLAAQSLKSKSQVVAMSLRNELRGPNQNVETWFQYMSQGAKLIH 209

Query: 181 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTF 240
           QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM D+WTSKPLNTF
Sbjct: 210 QINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWTSKPLNTF 269

Query: 241 CANVNQGFEDRAGFLVRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGL 300
           CA+VNQGFEDRAGFLVRG+NPIPLFVSEFGI+Q G NEGQNRFL+CFFTYLTENDFDWGL
Sbjct: 270 CASVNQGFEDRAGFLVRGKNPIPLFVSEFGIDQTGVNEGQNRFLNCFFTYLTENDFDWGL 329

Query: 301 WALQGSYYYREGVKNDEETFGVLDAKFTNVKNPK-FLQKFQLMQTKLQDPISNLTTSFIM 360
           WALQGSYYYREGVKN +E FGVLD+ F   KN K FL+KFQLMQ+KLQD  SNLTTSFIM
Sbjct: 330 WALQGSYYYREGVKNAKENFGVLDSTFAKAKNSKLFLKKFQLMQSKLQDSSSNLTTSFIM 389

Query: 361 YHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLAGSILCLQAVGDGLPPILSKD 420
           YHPLSG CVRMNKKYQLG+SSCKTSNRWSHEQDG PIKLAGSILCL+A+G GLPPILS+D
Sbjct: 390 YHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQD 449

Query: 421 CSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQW 480
           CSSQQS WKY S+AKLQLATVDE+GQ LCLQRASHSHQI+TNKC+C NDS+CQ DPQSQW
Sbjct: 450 CSSQQSIWKYGSDAKLQLATVDEQGQVLCLQRASHSHQIVTNKCLCSNDSQCQEDPQSQW 509

Query: 481 FTLVPSNVHL 489
           FTLVPSN+ L
Sbjct: 510 FTLVPSNLRL 519

The following BLAST results are available for this feature:

BLAST of Cucsat.G10257 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	3.2e-113	42.97	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P23548	3.7e-16	21.47	Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2	[more]
P19487	1.7e-05	20.53	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]

BLAST of Cucsat.G10257 vs. NCBI nr


Match Name	Identity	Description
KAE8648503.1	99.18	hypothetical protein Csa_009407 [Cucumis sativus]	[more]
KAE8648505.1	99.18	hypothetical protein Csa_007897 [Cucumis sativus]	[more]
XP_031739082.1	96.12	glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8650476.1 hypothetical...	[more]
KAA0042984.1	85.51	endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_038874277.1	85.31	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]

BLAST of Cucsat.G10257 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A5A7TI19	85.51	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7UGS0	86.48	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3BML8	85.10	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7UKF5	86.07	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7UKP6	84.90	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..342 e-value: 1.4E-63 score: 217.2
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 1..484
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 21..304 e-value: 1.5E-24 score: 87.0
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 2..336
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 353..463

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G10257.T1	Cucsat.G10257.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds