Cucsat.G10012 (gene) Cucumber (B10) v3

Overview
NameCucsat.G10012
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description60S ribosomal protein L3
Locationctg1673: 3268223 .. 3271762 (+)
RNA-Seq ExpressionCucsat.G10012
SyntenyCucsat.G10012
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCCTTCCCTCGCATCGCAGCCGCAGTATCGATTGTTCCTATTATAAATATAAAGGTGTAGCCTTAACCTAGTTACGACGCCAACAACCTCTCATCCCTTCAGCCATTTCAACTGAGGTTTCCATTGTCAGGTATACTTGTTTCAATGTGTATGGTGGCCTCTATGCTCATTTTACATCGTTCGATTAACTTCTTCTTGCAGGATCGTTCATCTTATCTTGCTTGATACAAGCTGGTTTTGTATTTCATTTCTTGCTGCTACGACGCTTATACATCTGGATGATTCGATGTACATGTTTTCTCATTTAACTTACTGACGACTGTTGTTCCATCATGTTTTTCTGGTCTAATTCCAATATTCCAAATGACAGTCAAAATGGTTTCCCCCTTAAGAAACTATTAATAATCAGGGCTGGAGTCTAGAACGGGCTCTTTCACCATTGCCCGTTTTTGGGTTTTTGGATTGAAACACAAGGGAGCCTGAGTTTTGGCACTATCTAGTGCCATAGGCCACTGGTCGGGGGTCATTTTGTTGTCTGATGGATTTCGGAACTTTGAACTCGGAGAAACTTGAACCTGGAACCTGTTTTGTTTTGCTAATCTTTCCAAATCTTATTCACATTCGTTTTTTTCAAAAAATTTTAACAATTGTTTTTCGCCACTTACGAACTCTATTATCCAAAACAGAACAAGTTCTGCTATTTTTTCTTCTCTTGATTTGACACATTCGTTCGATTTTATTTCATCCGAAACGTTTTTTTGTATCTCAATTTGAAAACTAATTTGTATTTACTGACCTTCTGTTTGATTCTAGCTAACGGAAATTCGGTTGATATGAAACTACTATGCCTTTATTTATTTTTCCTGTCATGGTTTTCTTCTGTTGTACATTGTTTTGTTTTCGGGTCTGGTGCCATCATTATATATTTATTGTGTTCTATGATAATATAATCATCTTTATCTCTATATAAGCGGAACCCAATTCATATTCTGGCCTTTTGGGACTCTTTTGGTTGTTCAATTAACATCATTAAAATATGGTAGTTCACTATTAATTGCTGTAGGAAGACATGTCTCACCGCAAGTTTGAGCACCCAAGACATGGCTCCTTAGGGTTTCTTCCAAGGAAGAGAGCTTCTCGGCATAGAGGAAAAGGTATTGGCAATCTTAACATTTATGCAGGGTGTATGGTTGCAGTTTATATGATCCCTGACTATTTTTTGTAATGTATTCATTGTTTATTAATCATAATCACTTCTTATTTGCATATAGTAGTTTCTGTTGATAGTATTTGTGTAATTCAATGAATATGGATTTTTCTTTTGCCGTTCATAATTTACTCTACGACCGTTCAAGTGCTTTTCTGAGTTTATTCTGTTATTATGGTAAGCTAAATGTTTTATTCTGTGTAATTATTGGCTCTCGATCAGAACATTTGTAGCATCGTCGTACTGCTGGATGCTGTTCTTGACTTAGTTTCATTAGAATTGCTTGTTTTGCCTTTGTTTTCATGCGTTTGCATTTTGGACGCATTTCAGTTGCATGATTATTTTCATACATTATCATTATCCTTGAATCATGTGTCAGTTCCAGTGAGCTAATTAATAAATATTGTCGTTGTAATTTGTGTTTTTCAGTGAAGGCGTTTCCTAGAGATGATTCATCAAAGCCTTGTAGGTTGACTGCTTTCCTTGGATACAAGGCTGGGATGACCCATATAGTCAGAGAAGTCGAAAAACCTGGTTCAAGTGAGTCATGTTCTAAGATATCTAAATCTAGTTTTGCAAAATGAGACAAATACGTATAGTAGTTTAAGTATCTGACGTGCAATTAGTTTTATTTCCATATTAAGCAGTTGTGTGGCTTGCTGTTTGCGGTGGACACTTGATTTAGATTTGATTAGACATTCATTTTTTTTACTATTGTTTTGTTAATCATTTATCTATTTCTGATCCTCGTATTGATTAGACTGAGACATCAACTTTTAATTTGAAAAGTACAATTTTTGGTAGGCAGGTTTATATTCTTTTGTGTTTTTAGGCGCTGTATTATTAATTTTAGTATAATGATTCTCTCAGAGCTACACAAGAAAGAAACATGTGAAGCTGTGACAATTATAGAGGCTCCCCCCATGGTGGTTGTTGGGGTTGTTGGCTACATCAGAACTCCCCGAGGTTTACGTTCATTTAAAACTGTGTGGGCTCAACACTTGAGCGAGGAGGTAAAGAGGCGATTTTACAAGAACTGGTGCATGTCAAAGAAAAAGGCTTTCACCAAGTACTCGAAGAAGTACGAGACTGAAGATGGTAAGAAAGATATTCAGTCACAGTTGGACAGTTTGAGGAAACACAGCACTGTAATTCGTGTTCTTGCCCATTCACAGGTATTTTATGTTTCCTTCTCATCTTTTTCAAAACATCTTATAGGCGATGTTGATCACCTGAAGGTCTAAACCGTTTTAAAATTTCGGTTCAGATTAGATATTTACCCTATGTTTAATAACCCATTTTCAAACCTTGTTTTTCAGATAAGAAAGATGCAGGGACTAAAGCAAAAGAAAGCCCACTTAATGGAAATTCAAGTGAATGGCGGAGATGTCCCTAAGAAGGTTGATTATGCATATAGTTTGTTTGAGAAGCAGGTCCCTGTTGATGCCATTTTCCAAAAGGATGAGATGATTGATATCATTGGAGTAACAAAGGGTAAAGGCTATGAAGGTGTTGTGACCCGTTGGGGTGTCACTCGCTTGCCCCGCAAGACCCATCGTGGCCTTCGGAAAGTAGCTTGTATTGGTGCTTGGCATCCAGCTAGAGTGTCGTTCACAGTTGCCAGGGCAGGACAGAATGGTTATCATCACCGCACAGAAATGAACAAAAAAGTTTATAAGATTGGGAAAACTGGACAGGATTCTCACTCCGCCATGACCGAATTTGATAGGTAAGCCTCTCAAAGCCAATCTTTCCTTGTGGATTATATTTTTACATCAATTCCTTGTTGTAGATTTCCCGCTGCTAATGCTTGTGGATATTTTATATAGGACTGAGAAGGATATAACTCCAATTGGTGGATTCCCTCACTATGGTATCGTCAAAGAGGATTACTTGATGATCAAGGGCGGTTGTGCAGGTCCAAAGAAGCGTGTGGTCACATTGAGGCAGTCACTGATCAAGCAAACATCTAGAGTTGCTATGGAGGAAATCAAACTGAAGTTCATCGATACATCGTCCAAGTTCGGCCACGGACGGTTCCAGACAACACAGGAGAAGGCTAGGTTTTATGGACGAGTCAAGTAGGTTTTATGGATCACTTTTTTCATTGGGGTTCTCTTCAATTTTTTCAGACTAATGTGACGAATATTCTTATCTTATGTTCGTATATTTTATTTTATTAATGAAAATATCCCATAGTTTATCAACATTAATTTAATTTTGGCATTTTGTTGGTGTCGTGATCCTTTCTCAATTGTTCGTATTGAATTCCTTCCATACTGAATGTGCTAACCGAACAAACCATTGGTTCATTGCATGTTTGTATGAGTTTCTATG

Coding sequence (CDS)

ATGTCTCACCGCAAGTTTGAGCACCCAAGACATGGCTCCTTAGGGTTTCTTCCAAGGAAGAGAGCTTCTCGGCATAGAGGAAAAGTGAAGGCGTTTCCTAGAGATGATTCATCAAAGCCTTGTAGGTTGACTGCTTTCCTTGGATACAAGGCTGGGATGACCCATATAGTCAGAGAAGTCGAAAAACCTGGTTCAAAGCTACACAAGAAAGAAACATGTGAAGCTGTGACAATTATAGAGGCTCCCCCCATGGTGGTTGTTGGGGTTGTTGGCTACATCAGAACTCCCCGAGGTTTACGTTCATTTAAAACTGTGTGGGCTCAACACTTGAGCGAGGAGGTAAAGAGGCGATTTTACAAGAACTGGTGCATGTCAAAGAAAAAGGCTTTCACCAAGTACTCGAAGAAGTACGAGACTGAAGATGGTAAGAAAGATATTCAGTCACAGTTGGACAGTTTGAGGAAACACAGCACTGTAATTCGTGTTCTTGCCCATTCACAGATAAGAAAGATGCAGGGACTAAAGCAAAAGAAAGCCCACTTAATGGAAATTCAAGTGAATGGCGGAGATGTCCCTAAGAAGGTTGATTATGCATATAGTTTGTTTGAGAAGCAGGTCCCTGTTGATGCCATTTTCCAAAAGGATGAGATGATTGATATCATTGGAGTAACAAAGGGTAAAGGCTATGAAGGTGTTGTGACCCGTTGGGGTGTCACTCGCTTGCCCCGCAAGACCCATCGTGGCCTTCGGAAAGTAGCTTGTATTGGTGCTTGGCATCCAGCTAGAGTGTCGTTCACAGTTGCCAGGGCAGGACAGAATGGTTATCATCACCGCACAGAAATGAACAAAAAAGTTTATAAGATTGGGAAAACTGGACAGGATTCTCACTCCGCCATGACCGAATTTGATAGGTAA

Protein sequence

MSHRKFEHPRHGSLGFLPRKRASRHRGKVKAFPRDDSSKPCRLTAFLGYKAGMTHIVREVEKPGSKLHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCMSKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEIQVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRKTHRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR
Homology
BLAST of Cucsat.G10012 vs. ExPASy Swiss-Prot
Match: P22738 (60S ribosomal protein L3-2 OS=Arabidopsis thaliana OX=3702 GN=ARP2 PE=2 SV=4)

HSP 1 Score: 575.1 bits (1481), Expect = 5.1e-163
Identity = 276/322 (85.71%), Postives = 306/322 (95.03%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIE PPMVVVGVVGY++TPRGLRS  TVWAQHLSEE++RRFYKNW  
Sbjct: 65  SKLHKKETCEAVTIIETPPMVVVGVVGYVKTPRGLRSLCTVWAQHLSEELRRRFYKNWAK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFT+YSKK+ETE+GKKDIQSQL+ ++K+ +VIRVLAH+QIRKM+GLKQKKAHL EI
Sbjct: 125 SKKKAFTRYSKKHETEEGKKDIQSQLEKMKKYCSVIRVLAHTQIRKMKGLKQKKAHLNEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           Q+NGGD+ KKVDYA SLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QINGGDIAKKVDYACSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVS+TVARAGQNGYHHRTEMNKKVY++GK GQ++HSAMTE+DR
Sbjct: 245 THRGLRKVACIGAWHPARVSYTVARAGQNGYHHRTEMNKKVYRVGKVGQETHSAMTEYDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITP+GGFPHYGIVKEDYLMIKG C GPKKRVVTLRQ+L+KQTSR+AMEEIKLKFID
Sbjct: 305 TEKDITPMGGFPHYGIVKEDYLMIKGCCVGPKKRVVTLRQTLLKQTSRLAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGR 326
            +S  GHGRFQT+QEKA+FYGR
Sbjct: 365 AASNGGHGRFQTSQEKAKFYGR 386

BLAST of Cucsat.G10012 vs. ExPASy Swiss-Prot
Match: P35684 (60S ribosomal protein L3 OS=Oryza sativa subsp. japonica OX=39947 GN=RPL3 PE=2 SV=2)

HSP 1 Score: 563.1 bits (1450), Expect = 2.0e-159
Identity = 262/324 (80.86%), Postives = 303/324 (93.52%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIE PP+V+VG+V Y++TPRGLRS  +VWAQHLSEEV+RRFYKNWC 
Sbjct: 65  SKLHKKETCEAVTIIETPPLVIVGLVAYVKTPRGLRSLNSVWAQHLSEEVRRRFYKNWCK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKY+ KY+++ GKK+IQ QL+ ++K+++++RV+AH+QIRKM+GLKQKKAHLMEI
Sbjct: 125 SKKKAFTKYALKYDSDAGKKEIQMQLEKMKKYASIVRVIAHTQIRKMKGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           Q+NGG +  KVDY Y  FEK++PVDA+FQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QINGGTIADKVDYGYKFFEKEIPVDAVFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVS+TVARAGQNGYHHRTEMNKKVYKIGK+GQ+SH+A TEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSYTVARAGQNGYHHRTEMNKKVYKIGKSGQESHAACTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITP+GGFPHYG+VK DYLMIKG C GPKKRVVTLRQSL+KQTSR+A+EEIKLKFID
Sbjct: 305 TEKDITPMGGFPHYGVVKGDYLMIKGCCVGPKKRVVTLRQSLLKQTSRLALEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 328
           TSSKFGHGRFQTT EK RF+G++K
Sbjct: 365 TSSKFGHGRFQTTDEKQRFFGKLK 388

BLAST of Cucsat.G10012 vs. ExPASy Swiss-Prot
Match: P17094 (60S ribosomal protein L3-1 OS=Arabidopsis thaliana OX=3702 GN=ARP1 PE=1 SV=5)

HSP 1 Score: 553.5 bits (1425), Expect = 1.6e-156
Identity = 259/323 (80.19%), Postives = 301/323 (93.19%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIE P MVVVGVV Y++TPRGLRS  TVWAQHLSEEV+RRFYKNW  
Sbjct: 65  SKLHKKETCEAVTIIETPAMVVVGVVAYVKTPRGLRSLNTVWAQHLSEEVRRRFYKNWAK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFT Y+K+Y++EDGKK IQ+QL+ ++K++TVIRVLAH+QIRKM+GLKQKKAH+MEI
Sbjct: 125 SKKKAFTGYAKQYDSEDGKKGIQAQLEKMKKYATVIRVLAHTQIRKMKGLKQKKAHMMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           Q+NGG + +KVD+AYS FEKQ+P++A+FQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QINGGTIAQKVDFAYSFFEKQIPIEAVFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVS+TVARAGQNGYHHRTE+NKK+Y++GK G ++H+AMTE+DR
Sbjct: 245 THRGLRKVACIGAWHPARVSYTVARAGQNGYHHRTELNKKIYRLGKVGTEAHTAMTEYDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKD+TP+GGFPHYGIVK+DYLMIKG C GPKKRVVTLRQSL+ QTSR+A+EEIKLKFID
Sbjct: 305 TEKDVTPMGGFPHYGIVKDDYLMIKGCCVGPKKRVVTLRQSLLTQTSRLALEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRV 327
           T+S FGHGRFQT+ EK RFY RV
Sbjct: 365 TASIFGHGRFQTSLEKMRFYNRV 387

BLAST of Cucsat.G10012 vs. ExPASy Swiss-Prot
Match: P49149 (60S ribosomal protein L3 OS=Toxocara canis OX=6265 GN=RPL3 PE=2 SV=2)

HSP 1 Score: 431.0 bits (1107), Expect = 1.2e-119
Identity = 205/327 (62.69%), Postives = 266/327 (81.35%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+++KKE  EAVTI EAPPMV+VG+VGYI TPRG R FKTVWA+HLSE+ + RFYKNW  
Sbjct: 65  SKVNKKEVVEAVTIFEAPPMVIVGIVGYIDTPRGPRQFKTVWAEHLSEDCRGRFYKNWHT 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAF K++KK++ EDG+K I++ L+ ++K+ + IRV+AH+Q++ M+  ++KKAH+MEI
Sbjct: 125 SKKKAFQKHAKKWQDEDGRKSIEADLNKMKKYCSKIRVIAHTQMKVMKH-REKKAHIMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           Q+NGG V +KVD+A    EKQVPVD++F +DEMID IGVTKGKG++GV +RW   +LPRK
Sbjct: 185 QINGGTVAEKVDWAREHLEKQVPVDSVFAQDEMIDCIGVTKGKGFKGVTSRWHTKKLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGK---TGQDSHSAMTE 243
           TH+GLRKVACIGAWHP+RV FTVARAGQ GYHHRTE+NKK+Y++GK   T +   +  T+
Sbjct: 245 THKGLRKVACIGAWHPSRVQFTVARAGQKGYHHRTEVNKKIYRLGKSCLTEEGKKNGGTD 304

Query: 244 FDRTEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLK 303
           +D TEK + P+GGFPHYG+V +D++MI+G C G KKR +TLR+SLI QT R A E+I LK
Sbjct: 305 YDITEKSVNPMGGFPHYGLVNQDFVMIRGCCVGSKKRPITLRKSLIVQTKRFAHEKINLK 364

Query: 304 FIDTSSKFGHGRFQTTQEKARFYGRVK 328
           +IDTSSKFGHGRFQT  EK  F G++K
Sbjct: 365 WIDTSSKFGHGRFQTHAEKRAFMGKLK 390

BLAST of Cucsat.G10012 vs. ExPASy Swiss-Prot
Match: Q8NKF4 (60S ribosomal protein L3 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=rpl3 PE=1 SV=2)

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-119
Identity = 213/324 (65.74%), Postives = 261/324 (80.56%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           +++HKKE  EAVTIIE PP+V VGVVGYI TPRGLRS  TVWA+HLS+EVKRRFYKNW  
Sbjct: 65  AKMHKKEIVEAVTIIETPPLVAVGVVGYIETPRGLRSLTTVWAEHLSDEVKRRFYKNWYK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKY+KK+  E+G   I  +L+ ++K+ TV+RVLAH+QIRK   LKQKKAHLMEI
Sbjct: 125 SKKKAFTKYAKKHAEENG-ASITRELERIKKYCTVVRVLAHTQIRKTP-LKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGG V  KVD+A +LFEK + +D+IF+KDEMID+I VTKG G++GV +RWG  +LPRK
Sbjct: 185 QVNGGSVADKVDFARNLFEKPIEIDSIFEKDEMIDVIAVTKGHGFQGVTSRWGTKKLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           TH+GLRKVACIGAWHP+ V +TVARAGQ GYHHRT  N KV++IGK G D  +A T+FD 
Sbjct: 245 THKGLRKVACIGAWHPSHVQWTVARAGQMGYHHRTSCNHKVFRIGK-GSDEGNASTDFDI 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           ++K ITP+GGF  YG VK DY+M+KG   G KKRV+TLR++L  QTSR A E+++LK+ID
Sbjct: 305 SKKQITPMGGFVRYGEVKNDYIMVKGSVPGVKKRVMTLRKTLYPQTSRRATEKVELKWID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 328
           TSSKFGHG FQT +EK  F G +K
Sbjct: 365 TSSKFGHGAFQTPEEKRAFMGTLK 385

BLAST of Cucsat.G10012 vs. NCBI nr
Match: XP_004135353.1 (60S ribosomal protein L3-2 [Cucumis sativus] >XP_031742245.1 60S ribosomal protein L3-2 [Cucumis sativus] >KGN51627.1 hypothetical protein Csa_007913 [Cucumis sativus])

HSP 1 Score: 647 bits (1668), Expect = 6.15e-233
Identity = 323/324 (99.69%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM
Sbjct: 65  SKLHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI
Sbjct: 125 SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKARFYGRVK
Sbjct: 365 TSSKFGHGRFQTTQEKARFYGRVK 388

BLAST of Cucsat.G10012 vs. NCBI nr
Match: XP_008445969.1 (PREDICTED: 60S ribosomal protein L3-2 [Cucumis melo] >KAA0034149.1 60S ribosomal protein L3-2 [Cucumis melo var. makuwa] >TYK15770.1 60S ribosomal protein L3-2 [Cucumis melo var. makuwa])

HSP 1 Score: 638 bits (1646), Expect = 1.38e-229
Identity = 318/324 (98.15%), Postives = 323/324 (99.69%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIEAPPMVVVGVVGYI+TPRGLRSFKTVWAQHLSEE+KRRFYKNWCM
Sbjct: 65  SKLHKKETCEAVTIIEAPPMVVVGVVGYIKTPRGLRSFKTVWAQHLSEELKRRFYKNWCM 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKYSKKYETEDGKKDIQSQL+SLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI
Sbjct: 125 SKKKAFTKYSKKYETEDGKKDIQSQLESLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYGIVKEDYLMIKG CAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGIVKEDYLMIKGCCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKA+FYGRVK
Sbjct: 365 TSSKFGHGRFQTTQEKAKFYGRVK 388

BLAST of Cucsat.G10012 vs. NCBI nr
Match: XP_023547361.1 (60S ribosomal protein L3-2 [Cucurbita pepo subsp. pepo] >XP_023547362.1 60S ribosomal protein L3-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 613 bits (1582), Expect = 7.78e-220
Identity = 302/324 (93.21%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIE PPMVVVGVVGY++TPRGLRS KTVWAQHLSEEVKRRFYKNWC 
Sbjct: 65  SKLHKKETCEAVTIIETPPMVVVGVVGYVKTPRGLRSLKTVWAQHLSEEVKRRFYKNWCK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAF+KYSKK+ETEDGKKDI +QL+ L+K+ TVIRVLAHSQIRKMQGLKQKKAHLMEI
Sbjct: 125 SKKKAFSKYSKKFETEDGKKDILAQLEKLKKYGTVIRVLAHSQIRKMQGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGGDV KKVDYAYSLFEKQ+PVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QVNGGDVSKKVDYAYSLFEKQIPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYK+GKTGQDSHSAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKVGKTGQDSHSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYG+VKEDYLMIKG C GPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGVVKEDYLMIKGCCVGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKA+FYGRVK
Sbjct: 365 TSSKFGHGRFQTTQEKAKFYGRVK 388

BLAST of Cucsat.G10012 vs. NCBI nr
Match: XP_022151895.1 (60S ribosomal protein L3-2 [Momordica charantia])

HSP 1 Score: 613 bits (1580), Expect = 1.63e-219
Identity = 298/324 (91.98%), Postives = 317/324 (97.84%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIE PPM+V+G+VGY++TPRGLRS KTVWAQHLSEEVKRRFYKNWC 
Sbjct: 65  SKLHKKETCEAVTIIETPPMIVIGIVGYVKTPRGLRSLKTVWAQHLSEEVKRRFYKNWCK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKYSKKYETEDGKKDIQ+Q + L+K+ST IRVLAHSQIRKM+GLKQKKAHLMEI
Sbjct: 125 SKKKAFTKYSKKYETEDGKKDIQAQFEKLKKYSTEIRVLAHSQIRKMKGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           +VNGGDVPKKVDYAYSLFEKQ+PVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 KVNGGDVPKKVDYAYSLFEKQIPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTE+NKK+YKIGKTGQDS+SAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTELNKKIYKIGKTGQDSYSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYG+VKEDYLM+KG CAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGVVKEDYLMMKGCCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKARFYGR+K
Sbjct: 365 TSSKFGHGRFQTTQEKARFYGRLK 388

BLAST of Cucsat.G10012 vs. NCBI nr
Match: KAG6601630.1 (60S ribosomal protein L3-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612 bits (1579), Expect = 2.23e-219
Identity = 301/324 (92.90%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIE PPMVVVGVVGY++TPRGLRS KTVWAQHLSEEVKRRFYKNWC 
Sbjct: 65  SKLHKKETCEAVTIIETPPMVVVGVVGYVKTPRGLRSLKTVWAQHLSEEVKRRFYKNWCK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAF+KYSKK+ETEDGKKDI +QL+ L+K+ TVIRVLAHSQIRKMQGLKQKKAHLMEI
Sbjct: 125 SKKKAFSKYSKKFETEDGKKDILAQLEKLKKYGTVIRVLAHSQIRKMQGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGGDV KKVDYAYSLFEKQ+PVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QVNGGDVSKKVDYAYSLFEKQIPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYK+GKTGQDSHSAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKVGKTGQDSHSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TE+DITPIGGFPHYG+VKEDYLMIKG C GPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TERDITPIGGFPHYGVVKEDYLMIKGCCVGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKA+FYGRVK
Sbjct: 365 TSSKFGHGRFQTTQEKAKFYGRVK 388

BLAST of Cucsat.G10012 vs. ExPASy TrEMBL
Match: A0A0A0KT85 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G586000 PE=3 SV=1)

HSP 1 Score: 647 bits (1668), Expect = 2.98e-233
Identity = 323/324 (99.69%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM
Sbjct: 65  SKLHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI
Sbjct: 125 SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKARFYGRVK
Sbjct: 365 TSSKFGHGRFQTTQEKARFYGRVK 388

BLAST of Cucsat.G10012 vs. ExPASy TrEMBL
Match: A0A5D3CWM8 (60S ribosomal protein L3-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002740 PE=3 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 6.70e-230
Identity = 318/324 (98.15%), Postives = 323/324 (99.69%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIEAPPMVVVGVVGYI+TPRGLRSFKTVWAQHLSEE+KRRFYKNWCM
Sbjct: 65  SKLHKKETCEAVTIIEAPPMVVVGVVGYIKTPRGLRSFKTVWAQHLSEELKRRFYKNWCM 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKYSKKYETEDGKKDIQSQL+SLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI
Sbjct: 125 SKKKAFTKYSKKYETEDGKKDIQSQLESLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYGIVKEDYLMIKG CAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGIVKEDYLMIKGCCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKA+FYGRVK
Sbjct: 365 TSSKFGHGRFQTTQEKAKFYGRVK 388

BLAST of Cucsat.G10012 vs. ExPASy TrEMBL
Match: A0A1S3BES9 (60S ribosomal protein L3-2 OS=Cucumis melo OX=3656 GN=LOC103488840 PE=3 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 6.70e-230
Identity = 318/324 (98.15%), Postives = 323/324 (99.69%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIEAPPMVVVGVVGYI+TPRGLRSFKTVWAQHLSEE+KRRFYKNWCM
Sbjct: 65  SKLHKKETCEAVTIIEAPPMVVVGVVGYIKTPRGLRSFKTVWAQHLSEELKRRFYKNWCM 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKYSKKYETEDGKKDIQSQL+SLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI
Sbjct: 125 SKKKAFTKYSKKYETEDGKKDIQSQLESLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYGIVKEDYLMIKG CAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGIVKEDYLMIKGCCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKA+FYGRVK
Sbjct: 365 TSSKFGHGRFQTTQEKAKFYGRVK 388

BLAST of Cucsat.G10012 vs. ExPASy TrEMBL
Match: A0A6J1DCG6 (60S ribosomal protein L3-2 OS=Momordica charantia OX=3673 GN=LOC111019751 PE=3 SV=1)

HSP 1 Score: 613 bits (1580), Expect = 7.88e-220
Identity = 298/324 (91.98%), Postives = 317/324 (97.84%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIE PPM+V+G+VGY++TPRGLRS KTVWAQHLSEEVKRRFYKNWC 
Sbjct: 65  SKLHKKETCEAVTIIETPPMIVIGIVGYVKTPRGLRSLKTVWAQHLSEEVKRRFYKNWCK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAFTKYSKKYETEDGKKDIQ+Q + L+K+ST IRVLAHSQIRKM+GLKQKKAHLMEI
Sbjct: 125 SKKKAFTKYSKKYETEDGKKDIQAQFEKLKKYSTEIRVLAHSQIRKMKGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           +VNGGDVPKKVDYAYSLFEKQ+PVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 KVNGGDVPKKVDYAYSLFEKQIPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTE+NKK+YKIGKTGQDS+SAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTELNKKIYKIGKTGQDSYSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYG+VKEDYLM+KG CAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGVVKEDYLMMKGCCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKARFYGR+K
Sbjct: 365 TSSKFGHGRFQTTQEKARFYGRLK 388

BLAST of Cucsat.G10012 vs. ExPASy TrEMBL
Match: A0A6J1K6W3 (60S ribosomal protein L3-2 OS=Cucurbita maxima OX=3661 GN=LOC111492834 PE=3 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 2.18e-219
Identity = 301/324 (92.90%), Postives = 314/324 (96.91%), Query Frame = 0

Query: 4   SELHKKETCEAVTIIEAPPMVVVGVVGYIRTPRGLRSFKTVWAQHLSEEVKRRFYKNWCM 63
           S+LHKKETCEAVTIIE PPMVVVGVVGY++TPRGLRS KTVWAQHLSEEVKRRFYKNWC 
Sbjct: 65  SKLHKKETCEAVTIIETPPMVVVGVVGYVKTPRGLRSLKTVWAQHLSEEVKRRFYKNWCK 124

Query: 64  SKKKAFTKYSKKYETEDGKKDIQSQLDSLRKHSTVIRVLAHSQIRKMQGLKQKKAHLMEI 123
           SKKKAF+KYSKK+ETEDGKKDI +Q + L+K+STVIRVLAHSQIRKMQGLKQKKAHLMEI
Sbjct: 125 SKKKAFSKYSKKFETEDGKKDILAQFEKLKKYSTVIRVLAHSQIRKMQGLKQKKAHLMEI 184

Query: 124 QVNGGDVPKKVDYAYSLFEKQVPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 183
           QVNGGDV KKVDYAYSLFEKQ+PVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK
Sbjct: 185 QVNGGDVSKKVDYAYSLFEKQIPVDAIFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRK 244

Query: 184 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKIGKTGQDSHSAMTEFDR 243
           THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYK+GKTGQDSHSAMTEFDR
Sbjct: 245 THRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKVYKVGKTGQDSHSAMTEFDR 304

Query: 244 TEKDITPIGGFPHYGIVKEDYLMIKGGCAGPKKRVVTLRQSLIKQTSRVAMEEIKLKFID 303
           TEKDITPIGGFPHYG+VKEDYLMIKG C GPKKRVVTLR SLIKQTSRVAMEEIKLKFID
Sbjct: 305 TEKDITPIGGFPHYGVVKEDYLMIKGCCVGPKKRVVTLRHSLIKQTSRVAMEEIKLKFID 364

Query: 304 TSSKFGHGRFQTTQEKARFYGRVK 327
           TSSKFGHGRFQTTQEKA+FYGRVK
Sbjct: 365 TSSKFGHGRFQTTQEKAKFYGRVK 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P227385.1e-16385.7160S ribosomal protein L3-2 OS=Arabidopsis thaliana OX=3702 GN=ARP2 PE=2 SV=4[more]
P356842.0e-15980.8660S ribosomal protein L3 OS=Oryza sativa subsp. japonica OX=39947 GN=RPL3 PE=2 S... [more]
P170941.6e-15680.1960S ribosomal protein L3-1 OS=Arabidopsis thaliana OX=3702 GN=ARP1 PE=1 SV=5[more]
P491491.2e-11962.6960S ribosomal protein L3 OS=Toxocara canis OX=6265 GN=RPL3 PE=2 SV=2[more]
Q8NKF41.5e-11965.7460S ribosomal protein L3 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 /... [more]
Match NameE-valueIdentityDescription
XP_004135353.16.15e-23399.6960S ribosomal protein L3-2 [Cucumis sativus] >XP_031742245.1 60S ribosomal prote... [more]
XP_008445969.11.38e-22998.15PREDICTED: 60S ribosomal protein L3-2 [Cucumis melo] >KAA0034149.1 60S ribosomal... [more]
XP_023547361.17.78e-22093.2160S ribosomal protein L3-2 [Cucurbita pepo subsp. pepo] >XP_023547362.1 60S ribo... [more]
XP_022151895.11.63e-21991.9860S ribosomal protein L3-2 [Momordica charantia][more]
KAG6601630.12.23e-21992.9060S ribosomal protein L3-2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A0A0KT852.98e-23399.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G586000 PE=3 SV=1[more]
A0A5D3CWM86.70e-23098.1560S ribosomal protein L3-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BES96.70e-23098.1560S ribosomal protein L3-2 OS=Cucumis melo OX=3656 GN=LOC103488840 PE=3 SV=1[more]
A0A6J1DCG67.88e-22091.9860S ribosomal protein L3-2 OS=Momordica charantia OX=3673 GN=LOC111019751 PE=3 S... [more]
A0A6J1K6W32.18e-21992.9060S ribosomal protein L3-2 OS=Cucurbita maxima OX=3661 GN=LOC111492834 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000597Ribosomal protein L3PFAMPF00297Ribosomal_L3coord: 1..304
e-value: 3.8E-155
score: 516.4
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 44..300
e-value: 5.5E-114
score: 382.4
NoneNo IPR availableGENE3D3.30.1430.10coord: 83..207
e-value: 5.5E-114
score: 382.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availablePANTHERPTHR11363:SF1360S RIBOSOMAL PROTEIN L3-1coord: 1..304
IPR044892Ribosomal protein L3, domain 3, archaeal type superfamilyGENE3D4.10.960.10Ribosomal protein L3, domain 3coord: 8..274
e-value: 5.5E-114
score: 382.4
IPR045077Ribosomal protein L3, archaeal/eukaryotic typePANTHERPTHR1136360S RIBOSOMAL PROTEIN L3-RELATEDcoord: 1..304
IPR019926Ribosomal protein L3, conserved sitePROSITEPS00474RIBOSOMAL_L3coord: 212..235
IPR009000Translation protein, beta-barrel domain superfamilySUPERFAMILY50447Translation proteinscoord: 4..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G10012.T7Cucsat.G10012.T7mRNA
Cucsat.G10012.T4Cucsat.G10012.T4mRNA
Cucsat.G10012.T1Cucsat.G10012.T1mRNA
Cucsat.G10012.T5Cucsat.G10012.T5mRNA
Cucsat.G10012.T3Cucsat.G10012.T3mRNA
Cucsat.G10012.T8Cucsat.G10012.T8mRNA
Cucsat.G10012.T10Cucsat.G10012.T10mRNA
Cucsat.G10012.T6Cucsat.G10012.T6mRNA
Cucsat.G10012.T2Cucsat.G10012.T2mRNA
Cucsat.G10012.T9Cucsat.G10012.T9mRNA
Cucsat.G10012.T11Cucsat.G10012.T11mRNA
Cucsat.G10012.T12Cucsat.G10012.T12mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000027 ribosomal large subunit assembly
biological_process GO:0006412 translation
cellular_component GO:0022625 cytosolic large ribosomal subunit
cellular_component GO:0005840 ribosome
molecular_function GO:0003723 RNA binding
molecular_function GO:0003735 structural constituent of ribosome