Csor.00g312930 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g312930
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	protease Do-like 8, chloroplastic
Location	Csor_Chr15: 1342121 .. 1344921 (+)
RNA-Seq Expression	Csor.00g312930
Synteny	Csor.00g312930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAGTTCTGGCATTCAATGCTCCCCAACTGCGAATGATGGTAGAATCTCGTGTTTGGACTTCGACCCATTTCTTGGGAAGACGAGAACTGTGCTTTGATGGTGTGTCGTCTGTATGCTCTCCTTCAAATCCCAGCAATTCCCAATCCATTTCCATCCTAAGGTTGTTTTTTCTCTCCTTTTTACTCTGTGCTACTGCATCATCTGGCCAAAATCTTTGTTCATCGTGTACCCATTGATATGCATTTAGGTGTTTTGGACTTTGTCTGAAGTGTGTAATTAGTGTAGCTGATTATTCAGGAATGCTGGTGATGACTCTGGAGATCTTTGTTCTATAATGAGCAGCAAATTTCTTTTCACAACTCGGCGGAGGTTATTTGCTAGTTTGTTTCTGAGTTTGTGGCCTTATCCTTCTAGGTTTGACTACTTTATGATCTCTGCACAATAAACTTGCATTTGGTTCTTCTACACGGGTCTCTATTTACGATAGTATGATATGCTCCACTTTGAGCATAAGCTCTTATGGCTTTGCTTTAGGCTTCCTCAAGAGGTCTCATTCCAATCGAGAGAATATTTTTTTGTTTATAAACCCATGATCATTCCCTAAATTAGCCGATGTGGGACTTTCATCATCCAACAGTCTCAACCTGTTGTGTTGTCGTGGATGATCGACGCTAACATTTATCATTTTTGTATGCGAAAACGAAATGTTGTGGTTAACTTCAAATGTGCAAATTTCTGCTCGGAGGGTTCTAAGAAGCATAATTGTTAATTTACTAGCTTTTTTAGCTTTATATGTATTATGTGAGATCGGGGAGTAGAACAAAACATTCATTATAAGGGTTTGGAAACCTCTCTCTAGCAGATGCATTTTAAAAACTTCGAGGGGAAGCCCGAAAGGAAAAGCCCAAAGAAGACAATATTTGCTAGCGATGGGTTTGGACCGTTACATGTTAGATACTTTCTTACACTTAAAAGAGAAAATCACTTATCTATAACCAAGTTGTGTAGTGTATTTACAGCTCAAGCTTTGGGCGATCCTTCTATAACCATAGATGAAGTAACCCCCACTTCTTCACCTTCTGGAGCCTTTTTCCCTACAGAGGTGTTTTTCTCTTCAATACATACTTATATATGCTTTCTTCCTAATTGATTTGTTTTGGATCTTCGTTGCGTTGTTATTATTTATCATGGTGTATGAACGAGAAATATGCGGTTGACAGGATAGAGTTGTCGAATCGTTTGAGAAAAATACATACTCTGTTGTCAACATTTTCGATGCGACTTTGCGTCCTCAACTCAATGTAACTGGAATGGTTGAGGTAATCATCTATATAAAACTATGGTTCATGTTGTTTTAGCCACTTTGACACGTTACGAATTGCATATCCCAAGCTGTTTATTGTGTTCCTATTTTGATAACCTTCATGTAAGATCATCATTTTGTATTCATTACCATGCCTATTTGCAGATTCCTGAAGGAAATGGGCCTGGAGTAGTCTGGGATGATCAGGGACATATAGTAACGAATTATCACGGTGAGTGCTTTTAGCGCCCGAGTAAAATGGTAACCATATTCGACTAGAAACAACTGTACATTGAATAGCTTCAGTGAGGATCATCTGACATTTCTAATGCCAAGCCTTGTATTCACCTAATGAACAGTAATTGCTAGTGCCCTTGCAAGAAATCCGAGCGCTGGGCAGGTTGTAGCACAAGTTAATATCCTAGCATCCGATGGGTGAGGACGCTTGAAGTTAGACCAATGTACTACTGTGATAAACAACATCTATCTGTTTCCTCAATTTTCTAAATCTTTGCATAGGATACAGAAGAATTTTGAAGGCAAGCTCGTTGGTGCAGATCGCACGAAGGATCTTGCCGTTTTAAAGGTAAGGCTTTTCATCTTGCAAGAATTTGATGATCAAGCATGCATGATTACCATTGTTATTCCAGAGAACAAACAAAACATCACTGATTCTAATCATATCTGAGATAATCTCTGCGACCGTGCAGGTGGATGCCTCGAACGATTTCTTGAGGCCAATCAAGGTGGGGCAGTCATCTTCTCTTAAAGTGGGGCGACACTGCTTGGCAATTGGAAATCCTTTTGGTTTTGATCATACTCTCGCAGTTGGAGTCATTAGTGGATTAAATCGCGACGTTTTTAGTCGGACTGGTGTCACCATCGGAGGTGGAATCGAAACGGATGCAGCTATCAATCCCGGGAATAGGTTAGACCAAGATTCATCCTAAAATGAATACCATCAGGGAAATCTTGTCTTTATCACCATATATATTTGATGTTAACATCAAGGAGATATCCTTTTCCTGTATCTTTGACAGTGGAGGTCCTCTACTTGATTCGAAAGGCAATTTAATCGGTATCAATACCGCTATTTTCAGTCGAACAGGTAACGTCCATCTTCCTCCTGCCAAGTGCCAATTCACTGTCTTACGTCCTTGCTGTAACGACAGGGTAGTTGTTGTGATTTTCACATTACCTCGTCCTGCTCTTCGACGACAACATTCAGAATGTGATTTAACATGACTCTACACTATCATGATTGTAGGAACATCAGCTGGTGTTGGATTTGCAATCCCATCTTCAACCGTAGTCAAGATTGTACCTCAGTTGATCCAATTGGGTAGAGTGAGTAAAGGTAAAGAGAGGAGGGCAAAGTTTGGAGCTGGCTTTGATATTAGAAGAAACTCGGTAGCAGAACGTCGCGGCGAAGAACACGAGCTAAGCATTTCGACATTCTACTTGGAATCAAATGGAGAAGAAGAAGAAGATTAA

mRNA sequence

ATGCAAGTTCTGGCATTCAATGCTCCCCAACTGCGAATGATGGTAGAATCTCGTGTTTGGACTTCGACCCATTTCTTGGGAAGACGAGAACTGTGCTTTGATGGTGTGTCGTCTGTATGCTCTCCTTCAAATCCCAGCAATTCCCAATCCATTTCCATCCTAAGGAATGCTGGTGATGACTCTGGAGATCTTTGTTCTATAATGAGCAGCAAATTTCTTTTCACAACTCGGCGGAGGTTATTTGCTAGTTTGTTTCTGAGTTTGTGGCCTTATCCTTCTAGTGTATTTACAGCTCAAGCTTTGGGCGATCCTTCTATAACCATAGATGAAGTAACCCCCACTTCTTCACCTTCTGGAGCCTTTTTCCCTACAGAGGATAGAGTTGTCGAATCGTTTGAGAAAAATACATACTCTGTTGTCAACATTTTCGATGCGACTTTGCGTCCTCAACTCAATGTAACTGGAATGGTTGAGATTCCTGAAGGAAATGGGCCTGGAGTAGTCTGGGATGATCAGGGACATATAGTAACGAATTATCACGTAATTGCTAGTGCCCTTGCAAGAAATCCGAGCGCTGGGCAGGTTGTAGCACAAGTTAATATCCTAGCATCCGATGGGATACAGAAGAATTTTGAAGGCAAGCTCGTTGGTGCAGATCGCACGAAGGATCTTGCCGTTTTAAAGGTGGATGCCTCGAACGATTTCTTGAGGCCAATCAAGGTGGGGCAGTCATCTTCTCTTAAAGTGGGGCGACACTGCTTGGCAATTGGAAATCCTTTTGGTTTTGATCATACTCTCGCAGTTGGAGTCATTAGTGGATTAAATCGCGACGTTTTTAGTCGGACTGGTGTCACCATCGGAGGTGGAATCGAAACGGATGCAGCTATCAATCCCGGGAATAGTGGAGGTCCTCTACTTGATTCGAAAGGCAATTTAATCGGTATCAATACCGCTATTTTCAGTCGAACAGGAACATCAGCTGGTGTTGGATTTGCAATCCCATCTTCAACCGTAGTCAAGATTGTACCTCAGTTGATCCAATTGGGTAGAGTGAGTAAAGGTAAAGAGAGGAGGGCAAAGTTTGGAGCTGGCTTTGATATTAGAAGAAACTCGGTAGCAGAACGTCGCGGCGAAGAACACGAGCTAAGCATTTCGACATTCTACTTGGAATCAAATGGAGAAGAAGAAGAAGATTAA

Coding sequence (CDS)

ATGCAAGTTCTGGCATTCAATGCTCCCCAACTGCGAATGATGGTAGAATCTCGTGTTTGGACTTCGACCCATTTCTTGGGAAGACGAGAACTGTGCTTTGATGGTGTGTCGTCTGTATGCTCTCCTTCAAATCCCAGCAATTCCCAATCCATTTCCATCCTAAGGAATGCTGGTGATGACTCTGGAGATCTTTGTTCTATAATGAGCAGCAAATTTCTTTTCACAACTCGGCGGAGGTTATTTGCTAGTTTGTTTCTGAGTTTGTGGCCTTATCCTTCTAGTGTATTTACAGCTCAAGCTTTGGGCGATCCTTCTATAACCATAGATGAAGTAACCCCCACTTCTTCACCTTCTGGAGCCTTTTTCCCTACAGAGGATAGAGTTGTCGAATCGTTTGAGAAAAATACATACTCTGTTGTCAACATTTTCGATGCGACTTTGCGTCCTCAACTCAATGTAACTGGAATGGTTGAGATTCCTGAAGGAAATGGGCCTGGAGTAGTCTGGGATGATCAGGGACATATAGTAACGAATTATCACGTAATTGCTAGTGCCCTTGCAAGAAATCCGAGCGCTGGGCAGGTTGTAGCACAAGTTAATATCCTAGCATCCGATGGGATACAGAAGAATTTTGAAGGCAAGCTCGTTGGTGCAGATCGCACGAAGGATCTTGCCGTTTTAAAGGTGGATGCCTCGAACGATTTCTTGAGGCCAATCAAGGTGGGGCAGTCATCTTCTCTTAAAGTGGGGCGACACTGCTTGGCAATTGGAAATCCTTTTGGTTTTGATCATACTCTCGCAGTTGGAGTCATTAGTGGATTAAATCGCGACGTTTTTAGTCGGACTGGTGTCACCATCGGAGGTGGAATCGAAACGGATGCAGCTATCAATCCCGGGAATAGTGGAGGTCCTCTACTTGATTCGAAAGGCAATTTAATCGGTATCAATACCGCTATTTTCAGTCGAACAGGAACATCAGCTGGTGTTGGATTTGCAATCCCATCTTCAACCGTAGTCAAGATTGTACCTCAGTTGATCCAATTGGGTAGAGTGAGTAAAGGTAAAGAGAGGAGGGCAAAGTTTGGAGCTGGCTTTGATATTAGAAGAAACTCGGTAGCAGAACGTCGCGGCGAAGAACACGAGCTAAGCATTTCGACATTCTACTTGGAATCAAATGGAGAAGAAGAAGAAGATTAA

Protein sequence

MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDDSGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGAFFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYHVIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIKVGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGNSGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAKFGAGFDIRRNSVAERRGEEHELSISTFYLESNGEEEED

Homology

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BLAST of Csor.00g312930 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 5.8e-121
Identity = 230/331 (69.49%), Postives = 268/331 (80.97%), Query Frame = 0


Query: 25  FLGRRELCFDGVSSVCSPSNPSNSQS---ISILRNAGDDSGDLCSIMSSKFLFTTRRRLF 84
           F+GRR+L    +SSVCS  +  +  S   +S ++   D   +L  +       TTRR L 
Sbjct: 16  FVGRRQL----LSSVCSKISQGDVVSHPPVSSVKVTQDWKSNLHELAVKSVPSTTRRILL 75

Query: 85  ASLFLSLWPYPSSVFTAQALGDPSI-TIDEVTPTSSPSGAFFPTEDRVVESFEKNTYSVV 144
            SLF++L   PS   +A ALGDPS+ T+++V+PT  P+G  FPTE R+V+ FEKNTYSVV
Sbjct: 76  TSLFMNLCFNPSRYLSALALGDPSVATVEDVSPTVFPAGPLFPTEGRIVQLFEKNTYSVV 135

Query: 145 NIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYHVIASALARNPSAGQVVAQVN 204
           NIFD TLRPQL +TG+VEIPEGNG GVVWD QG+IVTNYHVI +AL+RNPS G VV +VN
Sbjct: 136 NIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVN 195

Query: 205 ILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIKVGQSSSLKVGRHCLAIGNPF 264
           ILASDG+QKNFEGKLVGADR KDLAVLKVDA    L+PIKVGQS+SLKVG+ CLAIGNPF
Sbjct: 196 ILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPF 255

Query: 265 GFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGNSGGPLLDSKGNLIGINTAIF 324
           GFDHTL VGVISGLNRD+FS+TGVTIGGGI+TDAAINPGNSGGPLLDSKGNLIGINTAIF
Sbjct: 256 GFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIF 315

Query: 325 SRTGTSAGVGFAIPSSTVVKIVPQLIQLGRV 352
           ++TGTSAGVGFAIPSSTV+KIVPQLIQ  +V
Sbjct: 316 TQTGTSAGVGFAIPSSTVLKIVPQLIQFSKV 342

[Close]

BLAST of Csor.00g312930 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 217.6 bits (553), Expect = 2.5e-55
Identity = 119/226 (52.65%), Postives = 153/226 (67.70%), Query Frame = 0


Query: 129 VESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYHVIASALAR 188
           V  F++NT SVV I +  +R       ++E+P+G+G G VWD QGHIVTNYHVI  A   
Sbjct: 122 VRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGA--- 181

Query: 189 NPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIKVGQSSSLK 248
                   + + +  +D  Q  F+ K+VG D+ KD+AVL++DA  + LRPI VG S+ L 
Sbjct: 182 --------SDLRVTLAD--QTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLL 241

Query: 249 VGRHCLAIGNPFGFDHTLAVGVISGLNRDVFS-RTGVTIGGGIETDAAINPGNSGGPLLD 308
           VG+   AIGNPFG DHTL  GVISGL R++ S  TG  I   I+TDAAINPGNSGGPLLD
Sbjct: 242 VGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLD 301

Query: 309 SKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSK 354
           S G LIGINTAI+S +G S+GVGF+IP  TV  IV QL++ G+V++
Sbjct: 302 SSGTLIGINTAIYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTR 334

[Close]

BLAST of Csor.00g312930 vs. ExPASy Swiss-Prot
Match: Q9SEL7 (Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 SV=3)

HSP 1 Score: 189.5 bits (480), Expect = 7.3e-47
Identity = 109/232 (46.98%), Postives = 148/232 (63.79%), Query Frame = 0


Query: 125 EDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIP-----EGNGPGVVWDDQGHIVTNY 184
           E+R V  F+K + SVV I +A   P+ +   ++        EG G G VWD  GHIVTNY
Sbjct: 88  EERNVNLFQKTSPSVVYI-EAIELPKTSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNY 147

Query: 185 HVIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPI 244
           HVIA         G    +V+++ + G + + EGK+VG D   DLAVLK++     L P+
Sbjct: 148 HVIAK--LATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPV 207

Query: 245 KVGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPG 304
            +G S+ L+VG+ C AIGNP+G+++TL +GV+SGL R++ S  G +I   I+TDA IN G
Sbjct: 208 VLGTSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSG 267

Query: 305 NSGGPLLDSKGNLIGINTAIFSR--TGTSAGVGFAIPSSTVVKIVPQLIQLG 350
           NSGGPLLDS G+ IG+NTA F+R  +G S+GV FAIP  TVV+ VP LI  G
Sbjct: 268 NSGGPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYG 316

[Close]

BLAST of Csor.00g312930 vs. ExPASy Swiss-Prot
Match: P05676 (Uncharacterized serine protease syc0938_d OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=syc0938_d PE=3 SV=2)

HSP 1 Score: 144.4 bits (363), Expect = 2.7e-33
Identity = 87/204 (42.65%), Postives = 123/204 (60.29%), Query Frame = 0


Query: 151 LNVTGMVEIPEGNGPGVVWDDQGHIVTNYHVIASALARNPSAGQVVAQVNILASDGIQKN 210
           +N     E+  G G G V D  G I+TN HV+A+A            QV +   DG  + 
Sbjct: 112 VNPPARQEVQRGQGSGFVVDGNGLIMTNAHVVANA-----------DQVRVTLRDG--RE 171

Query: 211 FEGKLVGADRTKDLAVLKVDASNDFLRPIKVGQSSSLKVGRHCLAIGNPFGFDHTLAVGV 270
           F G++ GAD   DLA+++VD   + L   ++G SS+++VG   +AIGNP G D+T+ +G+
Sbjct: 172 FTGRVRGADSVTDLALVEVDTKGERLPTARIGNSSNVEVGDWAIAIGNPLGLDNTVTLGI 231

Query: 271 ISGLNRDVFSRTGVTIG--GGIETDAAINPGNSGGPLLDSKGNLIGINTAIFSRTGTSAG 330
           +S L R   S  G+       I+TDA INPGNSGGPL++S+G +IGINTAI  R    AG
Sbjct: 232 VSSLGRR-SSAVGIPDKRLDFIQTDAVINPGNSGGPLVNSRGEVIGINTAI--RQAPGAG 291

Query: 331 VGFAIPSSTVVKIVPQLIQLGRVS 353
           +GFAIP +T  +I  QL++ G+VS
Sbjct: 292 IGFAIPVNTAKQIETQLLKNGKVS 299

[Close]

BLAST of Csor.00g312930 vs. ExPASy Swiss-Prot
Match: F6AA62 (Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fulva (strain 12-X) OX=743720 GN=Psefu_3239 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.0e-32
Identity = 79/191 (41.36%), Postives = 116/191 (60.73%), Query Frame = 0


Query: 164 GPGVVWDDQGHIVTNYHVIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKD 223
           G G +    G+I+TN HV+A A            ++ +  SD  +   E KL+G D   D
Sbjct: 102 GSGFIISKDGYILTNNHVVADA-----------DEIIVRLSD--RSELEAKLIGTDPRSD 161

Query: 224 LAVLKVDASNDFLRPIKVGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTG 283
           +A+LKV+A++  L  +K+G S +LKVG   LAIG+PFGFDH++  G++S   R + + + 
Sbjct: 162 VALLKVEAND--LPTVKLGNSDNLKVGEWVLAIGSPFGFDHSVTAGIVSAKGRSLPNESY 221

Query: 284 VTIGGGIETDAAINPGNSGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVP 343
           V     I+TD AINPGNSGGPL +  G ++GIN+ IF+R+G   G+ FAIP S  + +  
Sbjct: 222 VPF---IQTDVAINPGNSGGPLFNLDGEVVGINSQIFTRSGGFMGLSFAIPMSVAMDVAD 274

Query: 344 QLIQLGRVSKG 355
           QL   G+VS+G
Sbjct: 282 QLKASGKVSRG 274

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BLAST of Csor.00g312930 vs. NCBI nr
Match: KAG6578588.1 (Protease Do-like 8, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 776 bits (2005), Expect = 6.90e-283
Identity = 398/398 (100.00%), Postives = 398/398 (100.00%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD
Sbjct: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
           SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA
Sbjct: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH
Sbjct: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN
Sbjct: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360
           SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK
Sbjct: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360

Query: 361 FGAGFDIRRNSVAERRGEEHELSISTFYLESNGEEEED 398
           FGAGFDIRRNSVAERRGEEHELSISTFYLESNGEEEED
Sbjct: 361 FGAGFDIRRNSVAERRGEEHELSISTFYLESNGEEEED 398

[Close]

BLAST of Csor.00g312930 vs. NCBI nr
Match: XP_023550587.1 (protease Do-like 8, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 742 bits (1916), Expect = 2.44e-269
Identity = 380/396 (95.96%), Postives = 387/396 (97.73%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQVLA+NAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILR+AGD+
Sbjct: 1   MQVLAYNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRSAGDE 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
           SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALG+PSITIDEVT T SPSGA
Sbjct: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGEPSITIDEVTSTFSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNG GVVWDDQGHIVTNYH
Sbjct: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGSGVVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVD  N+ LRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDTLNELLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           VGQSSSLKVG+HCLAIGNPFGFDHTL VGVISGLNRDVFS+TGVTIGGGIETDAAINPGN
Sbjct: 241 VGQSSSLKVGQHCLAIGNPFGFDHTLTVGVISGLNRDVFSQTGVTIGGGIETDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360
           SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK
Sbjct: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360

Query: 361 FGAGFDIRRNSVAERRGEEHELSISTFYLESNGEEE 396
           FGAGFDIRR SVAERRGEEHEL ISTFYLESNGEEE
Sbjct: 361 FGAGFDIRRKSVAERRGEEHELRISTFYLESNGEEE 396

[Close]

BLAST of Csor.00g312930 vs. NCBI nr
Match: XP_023550588.1 (protease Do-like 8, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731 bits (1887), Expect = 5.72e-265
Identity = 377/396 (95.20%), Postives = 384/396 (96.97%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQVLA+NAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILR+AGD+
Sbjct: 1   MQVLAYNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRSAGDE 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
           SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALG+PSITIDEVT T SPSGA
Sbjct: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGEPSITIDEVTSTFSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNG GVVWDDQGHIVTNYH
Sbjct: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGSGVVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVD  N+ LRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDTLNELLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           VGQSSSLKVG+HCLAIGNPFGFDHTL VGVISGLNRDVFS+TGVTIGGGIETDAAINPGN
Sbjct: 241 VGQSSSLKVGQHCLAIGNPFGFDHTLTVGVISGLNRDVFSQTGVTIGGGIETDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360
           SGGPLLDSKGNLIGINTAIFSRT   AGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK
Sbjct: 301 SGGPLLDSKGNLIGINTAIFSRT---AGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360

Query: 361 FGAGFDIRRNSVAERRGEEHELSISTFYLESNGEEE 396
           FGAGFDIRR SVAERRGEEHEL ISTFYLESNGEEE
Sbjct: 361 FGAGFDIRRKSVAERRGEEHELRISTFYLESNGEEE 393

[Close]

BLAST of Csor.00g312930 vs. NCBI nr
Match: XP_022993611.1 (protease Do-like 8, chloroplastic isoform X3 [Cucurbita maxima])

HSP 1 Score: 708 bits (1827), Expect = 8.87e-256
Identity = 365/396 (92.17%), Postives = 376/396 (94.95%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQ LA+N  QLRMMVESRVWTSTHFLGRREL FDGVSSV  PSNPSNSQSISILRNAGDD
Sbjct: 1   MQALAYNVQQLRMMVESRVWTSTHFLGRRELFFDGVSSVWCPSNPSNSQSISILRNAGDD 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
            GDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSI+IDEVTPT SPSGA
Sbjct: 61  PGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSISIDEVTPTFSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FF TEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEI EGNG GVVWDDQGHIVTNYH
Sbjct: 121 FFRTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEISEGNGSGVVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVD SN+FLRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDTSNNFLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           VGQSSSLKVG+ CLAIGNPFGF+HTL VGVI GLNRD+FS+TGVTIGGGI+TDAAINPGN
Sbjct: 241 VGQSSSLKVGQQCLAIGNPFGFNHTLTVGVIGGLNRDIFSQTGVTIGGGIQTDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360
           SGGPLLDSKG LIGINTAIF++TGTSAGVGFAIPS+TVVKIV QLIQLGRVSKGKE RAK
Sbjct: 301 SGGPLLDSKGYLIGINTAIFTQTGTSAGVGFAIPSATVVKIVSQLIQLGRVSKGKEMRAK 360

Query: 361 FGAGFDIRRNSVAERRGEEHELSISTFYLESNGEEE 396
           FGAGFDIRR SV ERRGEEHELSISTFYLESNGEEE
Sbjct: 361 FGAGFDIRRKSVVERRGEEHELSISTFYLESNGEEE 396

[Close]

BLAST of Csor.00g312930 vs. NCBI nr
Match: XP_022939766.1 (protease Do-like 8, chloroplastic [Cucurbita moschata])

HSP 1 Score: 699 bits (1803), Expect = 1.52e-252
Identity = 356/371 (95.96%), Postives = 363/371 (97.84%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQVLA+NAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSI ILRNAGDD
Sbjct: 1   MQVLAYNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSIPILRNAGDD 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
            GDLCSIM+SKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPT SPSGA
Sbjct: 61  YGDLCSIMTSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTFSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNG G+VWDDQGHIVTNYH
Sbjct: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGSGIVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNF GKLVGADRTKDLAVLKVDASNDFLRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFGGKLVGADRTKDLAVLKVDASNDFLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           V QSSSLKVG+HCLAIGNPFGFDHTLAVGVISGLNRDVFS+ GVTIGGGI+TDAAINPGN
Sbjct: 241 VEQSSSLKVGQHCLAIGNPFGFDHTLAVGVISGLNRDVFSQIGVTIGGGIQTDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360
           SGGPLLDSKGNLIGINTAIFS+TGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK
Sbjct: 301 SGGPLLDSKGNLIGINTAIFSQTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360

Query: 361 FGAGFDIRRNS 371
           FGAGFDIRR S
Sbjct: 361 FGAGFDIRRKS 371

[Close]

BLAST of Csor.00g312930 vs. ExPASy TrEMBL
Match: A0A6J1JWT2 (protease Do-like 8, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111489551 PE=3 SV=1)

HSP 1 Score: 708 bits (1827), Expect = 4.30e-256
Identity = 365/396 (92.17%), Postives = 376/396 (94.95%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQ LA+N  QLRMMVESRVWTSTHFLGRREL FDGVSSV  PSNPSNSQSISILRNAGDD
Sbjct: 1   MQALAYNVQQLRMMVESRVWTSTHFLGRRELFFDGVSSVWCPSNPSNSQSISILRNAGDD 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
            GDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSI+IDEVTPT SPSGA
Sbjct: 61  PGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSISIDEVTPTFSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FF TEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEI EGNG GVVWDDQGHIVTNYH
Sbjct: 121 FFRTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEISEGNGSGVVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVD SN+FLRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDTSNNFLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           VGQSSSLKVG+ CLAIGNPFGF+HTL VGVI GLNRD+FS+TGVTIGGGI+TDAAINPGN
Sbjct: 241 VGQSSSLKVGQQCLAIGNPFGFNHTLTVGVIGGLNRDIFSQTGVTIGGGIQTDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360
           SGGPLLDSKG LIGINTAIF++TGTSAGVGFAIPS+TVVKIV QLIQLGRVSKGKE RAK
Sbjct: 301 SGGPLLDSKGYLIGINTAIFTQTGTSAGVGFAIPSATVVKIVSQLIQLGRVSKGKEMRAK 360

Query: 361 FGAGFDIRRNSVAERRGEEHELSISTFYLESNGEEE 396
           FGAGFDIRR SV ERRGEEHELSISTFYLESNGEEE
Sbjct: 361 FGAGFDIRRKSVVERRGEEHELSISTFYLESNGEEE 396

[Close]

BLAST of Csor.00g312930 vs. ExPASy TrEMBL
Match: A0A6J1FGT8 (protease Do-like 8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445550 PE=3 SV=1)

HSP 1 Score: 699 bits (1803), Expect = 7.36e-253
Identity = 356/371 (95.96%), Postives = 363/371 (97.84%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQVLA+NAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSI ILRNAGDD
Sbjct: 1   MQVLAYNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSIPILRNAGDD 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
            GDLCSIM+SKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPT SPSGA
Sbjct: 61  YGDLCSIMTSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTFSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNG G+VWDDQGHIVTNYH
Sbjct: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGSGIVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNF GKLVGADRTKDLAVLKVDASNDFLRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFGGKLVGADRTKDLAVLKVDASNDFLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           V QSSSLKVG+HCLAIGNPFGFDHTLAVGVISGLNRDVFS+ GVTIGGGI+TDAAINPGN
Sbjct: 241 VEQSSSLKVGQHCLAIGNPFGFDHTLAVGVISGLNRDVFSQIGVTIGGGIQTDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360
           SGGPLLDSKGNLIGINTAIFS+TGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK
Sbjct: 301 SGGPLLDSKGNLIGINTAIFSQTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSKGKERRAK 360

Query: 361 FGAGFDIRRNS 371
           FGAGFDIRR S
Sbjct: 361 FGAGFDIRRKS 371

[Close]

BLAST of Csor.00g312930 vs. ExPASy TrEMBL
Match: A0A6J1JZ04 (protease Do-like 8, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489551 PE=3 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 5.95e-225
Identity = 323/351 (92.02%), Postives = 334/351 (95.16%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQ LA+N  QLRMMVESRVWTSTHFLGRREL FDGVSSV  PSNPSNSQSISILRNAGDD
Sbjct: 1   MQALAYNVQQLRMMVESRVWTSTHFLGRRELFFDGVSSVWCPSNPSNSQSISILRNAGDD 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
            GDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSI+IDEVTPT SPSGA
Sbjct: 61  PGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSISIDEVTPTFSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FF TEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEI EGNG GVVWDDQGHIVTNYH
Sbjct: 121 FFRTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEISEGNGSGVVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVD SN+FLRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDTSNNFLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           VGQSSSLKVG+ CLAIGNPFGF+HTL VGVI GLNRD+FS+TGVTIGGGI+TDAAINPGN
Sbjct: 241 VGQSSSLKVGQQCLAIGNPFGFNHTLTVGVIGGLNRDIFSQTGVTIGGGIQTDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRV 351
           SGGPLLDSKG LIGINTAIF++TGTSAGVGFAIPS+TVVKIV QLIQLGRV
Sbjct: 301 SGGPLLDSKGYLIGINTAIFTQTGTSAGVGFAIPSATVVKIVSQLIQLGRV 351

[Close]

BLAST of Csor.00g312930 vs. ExPASy TrEMBL
Match: A0A6J1K0M3 (protease Do-like 8, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489551 PE=3 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 1.78e-224
Identity = 323/351 (92.02%), Postives = 334/351 (95.16%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPSNPSNSQSISILRNAGDD 60
           MQ LA+N  QLRMMVESRVWTSTHFLGRREL FDGVSSV  PSNPSNSQSISILRNAGDD
Sbjct: 1   MQALAYNVQQLRMMVESRVWTSTHFLGRRELFFDGVSSVWCPSNPSNSQSISILRNAGDD 60

Query: 61  SGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTSSPSGA 120
            GDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSI+IDEVTPT SPSGA
Sbjct: 61  PGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSISIDEVTPTFSPSGA 120

Query: 121 FFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYH 180
           FF TEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEI EGNG GVVWDDQGHIVTNYH
Sbjct: 121 FFRTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEISEGNGSGVVWDDQGHIVTNYH 180

Query: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIK 240
           VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVD SN+FLRPIK
Sbjct: 181 VIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDTSNNFLRPIK 240

Query: 241 VGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGN 300
           VGQSSSLKVG+ CLAIGNPFGF+HTL VGVI GLNRD+FS+TGVTIGGGI+TDAAINPGN
Sbjct: 241 VGQSSSLKVGQQCLAIGNPFGFNHTLTVGVIGGLNRDIFSQTGVTIGGGIQTDAAINPGN 300

Query: 301 SGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRV 351
           SGGPLLDSKG LIGINTAIF++TGTSAGVGFAIPS+TVVKIV QLIQLGRV
Sbjct: 301 SGGPLLDSKGYLIGINTAIFTQTGTSAGVGFAIPSATVVKIVSQLIQLGRV 351

[Close]

BLAST of Csor.00g312930 vs. ExPASy TrEMBL
Match: A0A1S3C9Y8 (protease Do-like 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498478 PE=3 SV=1)

HSP 1 Score: 596 bits (1537), Expect = 6.32e-211
Identity = 307/356 (86.24%), Postives = 324/356 (91.01%), Query Frame = 0


Query: 1   MQVLAFNAPQLRMMVESRVWTSTHFLGRRELCFDGVSSVCSPS-----NPSNSQSISILR 60
           M VLA NAPQLRMM +S V TSTH LGRRELCFDGVSSVCS S     NPS S+SI I R
Sbjct: 1   MHVLACNAPQLRMMGKSGVSTSTHLLGRRELCFDGVSSVCSSSQPSTSNPSTSESIFIQR 60

Query: 61  NAGDDSGDLCSIMSSKFLFTTRRRLFASLFLSLWPYPSSVFTAQALGDPSITIDEVTPTS 120
            A DDSGDL S++S KFLFTTRR LF SL LSLWPYPSSVFTAQALGDPS+TIDEVTPT 
Sbjct: 61  RADDDSGDLGSVVSRKFLFTTRRTLFVSLSLSLWPYPSSVFTAQALGDPSVTIDEVTPTI 120

Query: 121 SPSGAFFPTEDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHI 180
           SPSG+ FPTE+R+ + FEKNTYSVVNIFD TLRPQLNVTGMVEIPEGNG GVVWDDQGHI
Sbjct: 121 SPSGSLFPTEERIAQLFEKNTYSVVNIFDVTLRPQLNVTGMVEIPEGNGSGVVWDDQGHI 180

Query: 181 VTNYHVIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDF 240
           VTNYHVIASALARNPSAGQVVA+VNILASDGIQKNFEGKL+GADRTKDLAVLKVDASND 
Sbjct: 181 VTNYHVIASALARNPSAGQVVARVNILASDGIQKNFEGKLIGADRTKDLAVLKVDASNDL 240

Query: 241 LRPIKVGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAA 300
           LRPIKVGQSSSLKVG+ CLAIGNPFGFDHTL VGVISGLNRD+FS+TGVTIGGGI+TDAA
Sbjct: 241 LRPIKVGQSSSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 300

Query: 301 INPGNSGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRV 351
           INPGNSGGPLLDSKGNLIGINTAIF++TGTSAGVGFAIPSSTVVKIVPQLIQ GRV
Sbjct: 301 INPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVVKIVPQLIQFGRV 356

[Close]

BLAST of Csor.00g312930 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 435.6 bits (1119), Expect = 4.2e-122
Identity = 230/331 (69.49%), Postives = 268/331 (80.97%), Query Frame = 0


Query: 25  FLGRRELCFDGVSSVCSPSNPSNSQS---ISILRNAGDDSGDLCSIMSSKFLFTTRRRLF 84
           F+GRR+L    +SSVCS  +  +  S   +S ++   D   +L  +       TTRR L 
Sbjct: 16  FVGRRQL----LSSVCSKISQGDVVSHPPVSSVKVTQDWKSNLHELAVKSVPSTTRRILL 75

Query: 85  ASLFLSLWPYPSSVFTAQALGDPSI-TIDEVTPTSSPSGAFFPTEDRVVESFEKNTYSVV 144
            SLF++L   PS   +A ALGDPS+ T+++V+PT  P+G  FPTE R+V+ FEKNTYSVV
Sbjct: 76  TSLFMNLCFNPSRYLSALALGDPSVATVEDVSPTVFPAGPLFPTEGRIVQLFEKNTYSVV 135

Query: 145 NIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYHVIASALARNPSAGQVVAQVN 204
           NIFD TLRPQL +TG+VEIPEGNG GVVWD QG+IVTNYHVI +AL+RNPS G VV +VN
Sbjct: 136 NIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVN 195

Query: 205 ILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIKVGQSSSLKVGRHCLAIGNPF 264
           ILASDG+QKNFEGKLVGADR KDLAVLKVDA    L+PIKVGQS+SLKVG+ CLAIGNPF
Sbjct: 196 ILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPF 255

Query: 265 GFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGNSGGPLLDSKGNLIGINTAIF 324
           GFDHTL VGVISGLNRD+FS+TGVTIGGGI+TDAAINPGNSGGPLLDSKGNLIGINTAIF
Sbjct: 256 GFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIF 315

Query: 325 SRTGTSAGVGFAIPSSTVVKIVPQLIQLGRV 352
           ++TGTSAGVGFAIPSSTV+KIVPQLIQ  +V
Sbjct: 316 TQTGTSAGVGFAIPSSTVLKIVPQLIQFSKV 342

[Close]

BLAST of Csor.00g312930 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 400.2 bits (1027), Expect = 1.9e-111
Identity = 216/331 (65.26%), Postives = 254/331 (76.74%), Query Frame = 0


Query: 25  FLGRRELCFDGVSSVCSPSNPSNSQS---ISILRNAGDDSGDLCSIMSSKFLFTTRRRLF 84
           F+GRR+L    +SSVCS  +  +  S   +S ++   D   +L  +       TTRR L 
Sbjct: 16  FVGRRQL----LSSVCSKISQGDVVSHPPVSSVKVTQDWKSNLHELAVKSVPSTTRRILL 75

Query: 85  ASLFLSLWPYPSSVFTAQALGDPSI-TIDEVTPTSSPSGAFFPTEDRVVESFEKNTYSVV 144
            SLF++L   PS   +A ALGDPS+ T+++V+PT  P+G  FPTE R+V+ FEKNTYSVV
Sbjct: 76  TSLFMNLCFNPSRYLSALALGDPSVATVEDVSPTVFPAGPLFPTEGRIVQLFEKNTYSVV 135

Query: 145 NIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYHVIASALARNPSAGQVVAQVN 204
           NIFD TLRPQL +TG+VEIPEGNG GVVWD QG+IVTNYHVI +AL+RNPS G VV +VN
Sbjct: 136 NIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVN 195

Query: 205 ILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIKVGQSSSLKVGRHCLAIGNPF 264
           ILASDG+QKNFEGKLVGADR KDLAVLKVDA    L+PIKVGQS+SLKVG+ CLAIGNPF
Sbjct: 196 ILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPF 255

Query: 265 GFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPGNSGGPLLDSKGNLIGINTAIF 324
           GFDHTL VGVISGLNRD+FS+TGVTIGGGI+TDAAINPGNSGGPLLDSKGNLIGINTAIF
Sbjct: 256 GFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIF 315

Query: 325 SRTGTSAGVGFAIPSSTVVKIVPQLIQLGRV 352
           ++              TV+KIVPQLIQ  +V
Sbjct: 316 TQ--------------TVLKIVPQLIQFSKV 328

[Close]

BLAST of Csor.00g312930 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 217.6 bits (553), Expect = 1.8e-56
Identity = 119/226 (52.65%), Postives = 153/226 (67.70%), Query Frame = 0


Query: 129 VESFEKNTYSVVNIFDATLRPQLNVTGMVEIPEGNGPGVVWDDQGHIVTNYHVIASALAR 188
           V  F++NT SVV I +  +R       ++E+P+G+G G VWD QGHIVTNYHVI  A   
Sbjct: 122 VRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGA--- 181

Query: 189 NPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPIKVGQSSSLK 248
                   + + +  +D  Q  F+ K+VG D+ KD+AVL++DA  + LRPI VG S+ L 
Sbjct: 182 --------SDLRVTLAD--QTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLL 241

Query: 249 VGRHCLAIGNPFGFDHTLAVGVISGLNRDVFS-RTGVTIGGGIETDAAINPGNSGGPLLD 308
           VG+   AIGNPFG DHTL  GVISGL R++ S  TG  I   I+TDAAINPGNSGGPLLD
Sbjct: 242 VGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLD 301

Query: 309 SKGNLIGINTAIFSRTGTSAGVGFAIPSSTVVKIVPQLIQLGRVSK 354
           S G LIGINTAI+S +G S+GVGF+IP  TV  IV QL++ G+V++
Sbjct: 302 SSGTLIGINTAIYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTR 334

[Close]

BLAST of Csor.00g312930 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 189.5 bits (480), Expect = 5.2e-48
Identity = 109/232 (46.98%), Postives = 148/232 (63.79%), Query Frame = 0


Query: 125 EDRVVESFEKNTYSVVNIFDATLRPQLNVTGMVEIP-----EGNGPGVVWDDQGHIVTNY 184
           E+R V  F+K + SVV I +A   P+ +   ++        EG G G VWD  GHIVTNY
Sbjct: 88  EERNVNLFQKTSPSVVYI-EAIELPKTSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNY 147

Query: 185 HVIASALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTKDLAVLKVDASNDFLRPI 244
           HVIA         G    +V+++ + G + + EGK+VG D   DLAVLK++     L P+
Sbjct: 148 HVIAK--LATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPV 207

Query: 245 KVGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRTGVTIGGGIETDAAINPG 304
            +G S+ L+VG+ C AIGNP+G+++TL +GV+SGL R++ S  G +I   I+TDA IN G
Sbjct: 208 VLGTSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSG 267

Query: 305 NSGGPLLDSKGNLIGINTAIFSR--TGTSAGVGFAIPSSTVVKIVPQLIQLG 350
           NSGGPLLDS G+ IG+NTA F+R  +G S+GV FAIP  TVV+ VP LI  G
Sbjct: 268 NSGGPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYG 316

[Close]

BLAST of Csor.00g312930 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 94.4 bits (233), Expect = 2.3e-19
Identity = 64/193 (33.16%), Postives = 106/193 (54.92%), Query Frame = 0


Query: 164 GPGVVWDDQGHIVTNYHVIAS-ALARNPSAGQVVAQVNILASDGIQKNFEGKLVGADRTK 223
           G G + D  G I+T  HV+      R+ S G    +V++   DG  + FEG +V AD   
Sbjct: 149 GSGTIIDADGTILTCAHVVVDFQNIRHSSKG----RVDVTLQDG--RTFEGVVVNADLQS 208

Query: 224 DLAVLKVDASNDFLRPIKVGQSSSLKVGRHCLAIGNPFGFDHTLAVGVISGLNRDVFSRT 283
           D+A++K+ +    L   K+G SS L+ G   +A+G P    +T+  G++S ++R     +
Sbjct: 209 DIALVKIKSKTP-LPTAKLGFSSKLRPGDWVIAVGCPLSLQNTVTAGIVSCVDR---KSS 268

Query: 284 GVTIGGG----IETDAAINPGNSGGPLLDSKGNLIGINTAIFSRTGTSAGVGFAIPSSTV 343
            + +GG     ++TD +IN GNSGGPL++  G +IG+N     +   + G+GF++P  +V
Sbjct: 269 DLGLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVN---IMKVLAADGLGFSVPIDSV 328

Query: 344 VKIVPQLIQLGRV 352
            KI+    + GRV
Sbjct: 329 SKIIEHFKKSGRV 328

The following BLAST results are available for this feature:

BLAST of Csor.00g312930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU10	5.8e-121	69.49	Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ...	[more]
O22609	2.5e-55	52.65	Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ...	[more]
Q9SEL7	7.3e-47	46.98	Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 ...	[more]
P05676	2.7e-33	42.65	Uncharacterized serine protease syc0938_d OS=Synechococcus sp. (strain ATCC 2714...	[more]
F6AA62	3.0e-32	41.36	Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fulva (strain ...	[more]

BLAST of Csor.00g312930 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578588.1	6.90e-283	100.00	Protease Do-like 8, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023550587.1	2.44e-269	95.96	protease Do-like 8, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023550588.1	5.72e-265	95.20	protease Do-like 8, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022993611.1	8.87e-256	92.17	protease Do-like 8, chloroplastic isoform X3 [Cucurbita maxima]	[more]
XP_022939766.1	1.52e-252	95.96	protease Do-like 8, chloroplastic [Cucurbita moschata]	[more]

BLAST of Csor.00g312930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JWT2	4.30e-256	92.17	protease Do-like 8, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1FGT8	7.36e-253	95.96	protease Do-like 8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445550 ...	[more]
A0A6J1JZ04	5.95e-225	92.02	protease Do-like 8, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1K0M3	1.78e-224	92.02	protease Do-like 8, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A1S3C9Y8	6.32e-211	86.24	protease Do-like 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498478 PE=3 S...	[more]

BLAST of Csor.00g312930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39830.1	4.2e-122	69.49	Trypsin family protein with PDZ domain	[more]
AT5G39830.2	1.9e-111	65.26	Trypsin family protein with PDZ domain	[more]
AT3G27925.1	1.8e-56	52.65	DegP protease 1	[more]
AT4G18370.1	5.2e-48	46.98	DEGP protease 5	[more]
AT5G27660.1	2.3e-19	33.16	Trypsin family protein with PDZ domain	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001940	Peptidase S1C	PRINTS	PR00834	PROTEASES2C	coord: 249..273 score: 46.54 coord: 286..303 score: 63.69 coord: 308..325 score: 48.51 coord: 173..185 score: 57.41
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 120..239 e-value: 1.7E-29 score: 103.8
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 240..353 e-value: 6.2E-38 score: 131.6
None	No IPR available	PFAM	PF13365	Trypsin_2	coord: 164..315 e-value: 3.4E-26 score: 93.0
None	No IPR available	PANTHER	PTHR43343	PEPTIDASE S12	coord: 61..352
None	No IPR available	PANTHER	PTHR43343:SF3	PROTEASE DO-LIKE 8, CHLOROPLASTIC	coord: 61..352
IPR009003	Peptidase S1, PA clan	SUPERFAMILY	50494	Trypsin-like serine proteases	coord: 135..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g312930.m01	Csor.00g312930.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010206	photosystem II repair
biological_process	GO:0006508	proteolysis
cellular_component	GO:0009543	chloroplast thylakoid lumen
molecular_function	GO:0004252	serine-type endopeptidase activity