Csor.00g312230 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g312230
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionProline dehydrogenase
LocationCsor_Chr15: 1039667 .. 1042604 (-)
RNA-Seq ExpressionCsor.00g312230
SyntenyCsor.00g312230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAGAAGTATCCTTTCAACCCAATCAAAGATCCCCAAGAACGTATTTTTTTACGCTTTCAATCGGTTGTTGAACACCGCCGCATCCTCCTCCGCCGCTGCCTTCACTTCCGCCGCTTCTACGCCGCCGCCGCATCTCAATCTCGCTGTCTCTCCGGCGGCACCCACCGTCGATTTTTCTGACACGAAGGCCTTGTTTGGGTCGTTATCAACGTTGGAGGTCCTACACGCCGCCGCCAGCCTCCATGTCGCCGCTGTCGGCCCTGTCGTAGACGTCGGAATGTGGGTTATGAACTCTAAATTGATGGATGTTGAGTTGGCTCGTGATGTAATTTTTGGTACCGTTAGACATAGTTTTTACCGACATTTTTGCGCCGGCGAGGACGACACGGCTGTCGGGAAAACCGTTCGCCGGTTGCATAATGTCGGGCTGAGAAGCATGCTCGATTACGCTATTGAATACGCCAACGATGAGGAGTCCTGTGATCGGAATCTTCATGGGTTTCTCCGAACCATTGAGGCCACTAGAGCGCTCCCACGTGGCTCTGTAAGTTCGTAAGAATTTTTTTTTGTTTAATACGGGAATAATATTGTCCACTTTGAGTATATGTTCATGATTTTGTTTTGGGTCTCTCCAAAATGTCTCAGACCACTTGTAAACGGCGATTCATTCTCTAAATTAGTCTACCTAGGACTCCCTCCCAACAATCTTCCGAGCCTTCCTTTGATGTCTATGTAGCCCTCGAACAGTTTCTCTTTAATCGAGGTTTAACTCTTGTCTCTGGAACCCTAGAACAAACTACACCCTTTGTTAACACAAGTTACTTTTGACTACACTTTCGATGCTAGTAATATCTTTGTTCTCATTCTCTAAATTAGTCTACATGGGACTCCCTCCCAACAATCTTCCGAGCCTTCCTTTAATGTCTATGTAGCCCTCGAACAGCTTCTCCTTAATCGAGGTTCAACTCTTGTCTCTAGAACCCGGGAACAAAGTACCCTTTTATTGACCCGAGTCACTTTTGCCTACACTTTTAAGGCTAGCAATTTCTTTATTCTTAAGGGCATGACTTTGATACCATGTTAGGAATCATACTCTCCACACGAGTATGATATTGTCTATTTTGAGTATAAGCTTCATGGCTTTGATTTGGGATTTTGGAAAGGGTCTCGTACCGATAGAGATGTATTCCTTACTTATAAACACATGATCATTCTCTAAATTAACGTTCGATTTGTTTCGTGCGATTGATAGGCAAGCTTTGTGGTGGCTAAAGTGAGCGCAATTTGCCCATTGAGATTGCTTGAGAGAGTGAGTGACTTGTTAAGATGGCAACAAAAGGACCCATGTTTGAATCTTCCATGGAAGCTCAAAACATTCCCAATTTTGTCTGAATCAAGCCCTCTTTACCACACTCTTCAACAGCCCAATCCCTTAACAAACGAAGAACAAAAAAGCCTCCAAATAAGCCATCAAAGGCTAATGAAAATCTGCCAAAGTTGCGTGGATGCCAACGTTCCTTTAGCCATCGACGCCGAGCACACCAAAATTCAACCCGCCATTGATTACTTCACATACTCCGCCGCCATCATCCACAACAAAGACCATAACCCAATAGTGTATGGAACCCTCCAAGCCTATCTAAAAGACGCAAAGGATCGGCTTTTGTTGGTCACCAAAGAGGCCAACGAAATGAGAGTTCCTCTTGGGATTAAGCTCGTGAGAGGTGCTTATATGTCTAGTGAATCCAAGCTCGCTTCTTCTCTTGGATTCGAATCTCCCATTCACAATAGCATTCATGACACACATGCATGCTATAATAGTTGCGCGTCGTTCTTGCTCGACAACATCGCCACAGGCTCGAGCGGAGCCATTCTCGCTACCCACAACGTCGAATCAGGTATCCAAAATCGTATCTCATTTAATAACATAGGTACCTAGAACCATGTCTCGTTTCATAACATGGATATCCAGAATCGTGTCTCGTTTAATAATATAGGAATCTAAAATCGTGCCTCGTTGAGAGTACCCAGAATCGTGTCTCATTTAATAACATAGGTACCTAGAATCATGTCTCGTTTCATAACATGGGTATCGAGAATCGTGTCTCGTTTCATAACATGGGTATCCTGTCTCGTTATGGGAACCCAAAATCGTGTCTCATTAAGAGTACTCAGAATCGTGTCTTGTTTAATAACATGGGTACCCAGAACCGTGTCTCGTTTCATAACATGGGTATCCAGAATCGTGTCTCGTTTAATAACATGGGAATCCAGAATCGTGTCTCTTTGAATAACATGAGTACCCAGAATCGTGTCTCATTTAATAACATGGGTACCCCCCAGAATCGTGTCTCGTTTTAATGAGTACCCAGAATTGTGTCTCTGTTTAATAACATGGGTACCCAAAATCATGTTTCGTGTAATAACCATGTTAGAACGTTGTTCATAATCTAGATCTCGTTCGATAATCATTTTATACCCACAATGTCGAATCCTGTAACACCCATATTAGAACGTTATTCATAATATGGGTCTCGTTTGATAACCATTCTACAGGAAAATTAGCTGCAAAAAAAGCGTACGAGATAGGGATTGAGAAGTTGAAGAAAAAGCTCGAGTTTGCTCAATTGTATGGAATGTCGGAAGCGTTGTCGTATGGGTTAAGAAACGCTGGGTTTCAAGTAAGCAAATACATGCCATTTGGAGCAGTGGATATGGTAATGCCATACCTTCTAAGAAGAGCAGAGGAGAATAGAGGCCTCTTATCGGCCTCAAATTTGGACAGACAGCTTATGAGGTATAGAAATATAAATATTTTGTAGGGAGGGACTTGATAGCAAATTTGACATCTTTTTTAATTTCTTGATATTTGCAGAAAGGAGCTCGGGAGGAGGATGAAAGAGTACTTGAGGGGTGAAAGAAGGGCCTTTTAG

mRNA sequence

ATGGCCAGAAGTATCCTTTCAACCCAATCAAAGATCCCCAAGAACGTATTTTTTTACGCTTTCAATCGGTTGTTGAACACCGCCGCATCCTCCTCCGCCGCTGCCTTCACTTCCGCCGCTTCTACGCCGCCGCCGCATCTCAATCTCGCTGTCTCTCCGGCGGCACCCACCGTCGATTTTTCTGACACGAAGGCCTTGTTTGGGTCGTTATCAACGTTGGAGGTCCTACACGCCGCCGCCAGCCTCCATGTCGCCGCTGTCGGCCCTGTCGTAGACGTCGGAATGTGGGTTATGAACTCTAAATTGATGGATGTTGAGTTGGCTCGTGATGTAATTTTTGGTACCGTTAGACATAGTTTTTACCGACATTTTTGCGCCGGCGAGGACGACACGGCTGTCGGGAAAACCGTTCGCCGGTTGCATAATGTCGGGCTGAGAAGCATGCTCGATTACGCTATTGAATACGCCAACGATGAGGAGTCCTGTGATCGGAATCTTCATGGGTTTCTCCGAACCATTGAGGCCACTAGAGCGCTCCCACGTGGCTCTGCAAGCTTTGTGGTGGCTAAAGTGAGCGCAATTTGCCCATTGAGATTGCTTGAGAGAGTGAGTGACTTGTTAAGATGGCAACAAAAGGACCCATGTTTGAATCTTCCATGGAAGCTCAAAACATTCCCAATTTTGTCTGAATCAAGCCCTCTTTACCACACTCTTCAACAGCCCAATCCCTTAACAAACGAAGAACAAAAAAGCCTCCAAATAAGCCATCAAAGGCTAATGAAAATCTGCCAAAGTTGCGTGGATGCCAACGTTCCTTTAGCCATCGACGCCGAGCACACCAAAATTCAACCCGCCATTGATTACTTCACATACTCCGCCGCCATCATCCACAACAAAGACCATAACCCAATAGTGTATGGAACCCTCCAAGCCTATCTAAAAGACGCAAAGGATCGGCTTTTGTTGGTCACCAAAGAGGCCAACGAAATGAGAGTTCCTCTTGGGATTAAGCTCGTGAGAGGTGCTTATATGTCTAGTGAATCCAAGCTCGCTTCTTCTCTTGGATTCGAATCTCCCATTCACAATAGCATTCATGACACACATGCATGCTATAATAGTTGCGCGTCGTTCTTGCTCGACAACATCGCCACAGGCTCGAGCGGAGCCATTCTCGCTACCCACAACGTCGAATCAGGAAAATTAGCTGCAAAAAAAGCGTACGAGATAGGGATTGAGAAGTTGAAGAAAAAGCTCGAGTTTGCTCAATTGTATGGAATGTCGGAAGCGTTGTCGTATGGGTTAAGAAACGCTGGGTTTCAAGTAAGCAAATACATGCCATTTGGAGCAGTGGATATGGTAATGCCATACCTTCTAAGAAGAGCAGAGGAGAATAGAGGCCTCTTATCGGCCTCAAATTTGGACAGACAGCTTATGAGAAAGGAGCTCGGGAGGAGGATGAAAGAGTACTTGAGGGGTGAAAGAAGGGCCTTTTAG

Coding sequence (CDS)

ATGGCCAGAAGTATCCTTTCAACCCAATCAAAGATCCCCAAGAACGTATTTTTTTACGCTTTCAATCGGTTGTTGAACACCGCCGCATCCTCCTCCGCCGCTGCCTTCACTTCCGCCGCTTCTACGCCGCCGCCGCATCTCAATCTCGCTGTCTCTCCGGCGGCACCCACCGTCGATTTTTCTGACACGAAGGCCTTGTTTGGGTCGTTATCAACGTTGGAGGTCCTACACGCCGCCGCCAGCCTCCATGTCGCCGCTGTCGGCCCTGTCGTAGACGTCGGAATGTGGGTTATGAACTCTAAATTGATGGATGTTGAGTTGGCTCGTGATGTAATTTTTGGTACCGTTAGACATAGTTTTTACCGACATTTTTGCGCCGGCGAGGACGACACGGCTGTCGGGAAAACCGTTCGCCGGTTGCATAATGTCGGGCTGAGAAGCATGCTCGATTACGCTATTGAATACGCCAACGATGAGGAGTCCTGTGATCGGAATCTTCATGGGTTTCTCCGAACCATTGAGGCCACTAGAGCGCTCCCACGTGGCTCTGCAAGCTTTGTGGTGGCTAAAGTGAGCGCAATTTGCCCATTGAGATTGCTTGAGAGAGTGAGTGACTTGTTAAGATGGCAACAAAAGGACCCATGTTTGAATCTTCCATGGAAGCTCAAAACATTCCCAATTTTGTCTGAATCAAGCCCTCTTTACCACACTCTTCAACAGCCCAATCCCTTAACAAACGAAGAACAAAAAAGCCTCCAAATAAGCCATCAAAGGCTAATGAAAATCTGCCAAAGTTGCGTGGATGCCAACGTTCCTTTAGCCATCGACGCCGAGCACACCAAAATTCAACCCGCCATTGATTACTTCACATACTCCGCCGCCATCATCCACAACAAAGACCATAACCCAATAGTGTATGGAACCCTCCAAGCCTATCTAAAAGACGCAAAGGATCGGCTTTTGTTGGTCACCAAAGAGGCCAACGAAATGAGAGTTCCTCTTGGGATTAAGCTCGTGAGAGGTGCTTATATGTCTAGTGAATCCAAGCTCGCTTCTTCTCTTGGATTCGAATCTCCCATTCACAATAGCATTCATGACACACATGCATGCTATAATAGTTGCGCGTCGTTCTTGCTCGACAACATCGCCACAGGCTCGAGCGGAGCCATTCTCGCTACCCACAACGTCGAATCAGGAAAATTAGCTGCAAAAAAAGCGTACGAGATAGGGATTGAGAAGTTGAAGAAAAAGCTCGAGTTTGCTCAATTGTATGGAATGTCGGAAGCGTTGTCGTATGGGTTAAGAAACGCTGGGTTTCAAGTAAGCAAATACATGCCATTTGGAGCAGTGGATATGGTAATGCCATACCTTCTAAGAAGAGCAGAGGAGAATAGAGGCCTCTTATCGGCCTCAAATTTGGACAGACAGCTTATGAGAAAGGAGCTCGGGAGGAGGATGAAAGAGTACTTGAGGGGTGAAAGAAGGGCCTTTTAG

Protein sequence

MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSFYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALPRGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRMKEYLRGERRAF
Homology
BLAST of Csor.00g312230 vs. ExPASy Swiss-Prot
Match: P92983 (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 PE=1 SV=2)

HSP 1 Score: 494.2 bits (1271), Expect = 1.7e-138
Identity = 249/464 (53.66%), Postives = 331/464 (71.34%), Query Frame = 0

Query: 26  NTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDFSDTKALFGSLSTLEVLHAAASLHVA 85
           +TA      +F   A  PP H       +   +D SD   LF S+ T ++L + A LH A
Sbjct: 31  STAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRSTAVLHAA 90

Query: 86  AVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSFYRHFCAGEDDTAVGKTVRRLHN-VG 145
           A+GP+VD+G WVM+SKLMD  + R ++ G V+ +FY HFCAGED  A  + VR ++   G
Sbjct: 91  AIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRSVYEATG 150

Query: 146 LRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALPRGSASFVVAKVSAICPLRLLERVS 205
           L+ ML Y +E+A+D  SCD N+  F+RTIEA ++LP    S VV K++AICP+ LL+RVS
Sbjct: 151 LKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPISLLKRVS 210

Query: 206 DLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQ 265
           DLLRW+ K P   L WKLK+FP+ SESSPLYHT  +P PLT EE++ L+ +H R+ +IC+
Sbjct: 211 DLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGRIQEICR 270

Query: 266 SCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHN-PIVYGTLQAYLKDAKDRLLLV 325
            C ++NVPL IDAE T +QPAIDY  YS+AI+ N D + PIVY T+QAYL+DA +RL L 
Sbjct: 271 KCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAGERLHLA 330

Query: 326 TKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIA 385
            + A +  VP+G KLVRGAYMSSE+ LA SLG +SP+H++I DTH+CYN C +FL++  +
Sbjct: 331 VQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTFLMEKAS 390

Query: 386 TGSS-GAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVS 445
            GS  G +LATHN +SG+LA++KA ++GI+K   K+EFAQLYGMS+ALS+GL+ AGF VS
Sbjct: 391 NGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKRAGFNVS 450

Query: 446 KYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRM 487
           KYMPFG V   +PYLLRRA ENRG+++    DRQLMR EL RR+
Sbjct: 451 KYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMRMELKRRL 494

BLAST of Csor.00g312230 vs. ExPASy Swiss-Prot
Match: Q6NKX1 (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 PE=2 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 4.7e-136
Identity = 238/432 (55.09%), Postives = 315/432 (72.92%), Query Frame = 0

Query: 58  VDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVR 117
           +D SD   LF S+    +L + A LH  ++GP+VD+G W+M+SKLMD  + RD++   V+
Sbjct: 42  LDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDLVLRIVK 101

Query: 118 HSFYRHFCAGEDDTAVGKTVRRLH-NVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEAT 177
            +FY HFCAGED  A  + V  ++ + GL+ ML Y +E+A D  +CD N+  F+ T+EA 
Sbjct: 102 GTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFIETVEAA 161

Query: 178 RALPRGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYH 237
           + LP    S VV K++AICP+ +L+RVSDLLRWQ K+P   LPWKL +FP+ S  SPLYH
Sbjct: 162 KTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYH 221

Query: 238 TLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAII 297
           T  +P PLT EE++ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  Y +AI+
Sbjct: 222 TTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIM 281

Query: 298 HNKDHN-PIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLG 357
            N D + PIVY T+QAYLKDA +RL L  +E+ +M VP+G KLVRGAYMSSE+KLA SLG
Sbjct: 282 FNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLG 341

Query: 358 FESPIHNSIHDTHACYNSCASFLLDNIATGSS-GAILATHNVESGKLAAKKAYEIGIEKL 417
           ++SP+H++I +TH CYN C SFL++  + GS    ILATHN +SGKL A+KA E+GI K 
Sbjct: 342 YKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKE 401

Query: 418 KKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLD 477
             K+EFAQLYGMS+ALS+GL+ AGF VSKYMP+G VD  +PYL+RRA ENRG++S   LD
Sbjct: 402 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALD 461

Query: 478 RQLMRKELGRRM 487
           RQLMRKEL RR+
Sbjct: 462 RQLMRKELKRRV 473

BLAST of Csor.00g312230 vs. ExPASy Swiss-Prot
Match: Q86H28 (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=prodh PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.7e-48
Identity = 143/441 (32.43%), Postives = 230/441 (52.15%), Query Frame = 0

Query: 62  DTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSFY 121
           DT  L+ S ST E+      L V ++  + D     +N     + L++ + F  +++SF+
Sbjct: 145 DTSKLYVSKSTGELFFTFMILKVCSINFISDNSQKFLN-LFEKLGLSKPLNF-FIKYSFF 204

Query: 122 RHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAI-EYANDEESCDRNLHGFLRTIEATRALP 181
           + FCAGE          +L+ +G+ ++LDYAI E A   E  D        TI      P
Sbjct: 205 KQFCAGETIRETEIFTEKLNKLGIGTILDYAIEELAGSSEGFDSVAENICETIRVAAKNP 264

Query: 182 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 241
             S S V  K + +    +LE+++ L+         N+   +   PI S +SPL   L Q
Sbjct: 265 TNSFSCV--KFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLDFYLNQ 324

Query: 242 PNP---------LTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTY 301
            +          LT+++ K ++    R+ KI Q C    VP+ +DAE +  Q AI + T 
Sbjct: 325 SSSLMKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTM 384

Query: 302 SAAIIHNKDHNPIVYGTLQAYLKDAKDRLL--LVTKEANEMRVPLGIKLVRGAYMSSESK 361
           S +I +NK+  PI+Y T Q YL +  + L        + +    LG K+VRGAYM +ES+
Sbjct: 385 SYSIKYNKE-KPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESE 444

Query: 362 LASSLGFESPIHNSIHDTHACYNSCASFLLDNIAT--GSSGAILATHNVESGKLAAKKAY 421
            +  L  E+P+  +I DTH  YN+   FLL+ I +   S G ++A+HN +S  L  K   
Sbjct: 445 RSQRLSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIK 504

Query: 422 EIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGL 481
           +  I+     ++F QL+GM++ LS+ L +   ++ KY+PFG V+ V+PYL+RR  EN+G 
Sbjct: 505 QYKIDPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKGF 564

Query: 482 LSASNLDRQL--MRKELGRRM 487
           +  SN D++L  ++KE+ RR+
Sbjct: 565 I-GSNSDKELFYLKKEIKRRL 571

BLAST of Csor.00g312230 vs. ExPASy Swiss-Prot
Match: A6QQ74 (Hydroxyproline dehydrogenase OS=Bos taurus OX=9913 GN=PRODH2 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.7e-38
Identity = 132/430 (30.70%), Postives = 215/430 (50.00%), Query Frame = 0

Query: 74  EVLHAAASLHVAAVGPVVDVGMWVM--NSKLMDVELARDVIFGTVRHSFYRHFCAGEDDT 133
           E+  A   L + A  P+V  G+ +   + +L+   L+  ++    R S Y  F AGE   
Sbjct: 36  ELTRALLVLRLCAWPPLVTHGLALQAWSQRLLGSRLSGALL----RASIYGQFVAGETAE 95

Query: 134 AVGKTVRRLHNVGLRSMLDYAIEYAND------EESCDRNLHGFLRTIEATRAL-----P 193
            V   V +L N+GLR +L    E   D      E   + NL   LR ++ +R L     P
Sbjct: 96  EVRSCVLQLQNLGLRPLLAVPTEEEPDSAVKTGEAWYEGNLSAMLRCVDLSRGLLETPDP 155

Query: 194 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 253
            G+A  +  K++A+   RL ++++  +R +  D     P +      L+E+      L Q
Sbjct: 156 TGNA-LMQLKMTALMSTRLCKQLTSWVR-RPGDSLELSPER------LAEAMDSGQDL-Q 215

Query: 254 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK- 313
            + L  E+ + LQ S  RL ++ Q      V L +DAE+T + PA+     + A   N  
Sbjct: 216 VSCLNTEQTRHLQASLSRLHRVVQHARAQRVRLLVDAEYTSLNPALSLLVAALATRWNSS 275

Query: 314 -DHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFES 373
            +  P V+ T QAYLKD  +RL    + A+   +  G+KLVRGAY+  E + A   G E 
Sbjct: 276 GEGGPWVWNTYQAYLKDTYERLRWDAEAADRAGLAFGVKLVRGAYLDKERETARLQGTED 335

Query: 374 PIHNSIHDTHACYNSCASFLLDNIATGS--SGAILATHNVESGKLAAKKAYEIGIEKLKK 433
           P       T   Y+ C   +L  ++        ++A+HN +S + A K+ +E+GI     
Sbjct: 336 PTQPDYEATSQSYSRCLELMLTQVSHRGPMCHLMVASHNEDSVRQATKRMWELGIPP-DG 395

Query: 434 KLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQ 487
            + F QL GM + +S  L  AG+ V K +P+G+++ V+PYL+RRA+ENR +L  +  +++
Sbjct: 396 PVCFGQLLGMCDHVSLALGQAGYAVYKSIPYGSLEEVIPYLIRRAQENRSVLRGARREQE 451

BLAST of Csor.00g312230 vs. ExPASy Swiss-Prot
Match: O43272 (Proline dehydrogenase 1, mitochondrial OS=Homo sapiens OX=9606 GN=PRODH PE=1 SV=3)

HSP 1 Score: 159.8 bits (403), Expect = 7.7e-38
Identity = 151/580 (26.03%), Postives = 250/580 (43.10%), Query Frame = 0

Query: 25  LNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDFSDTKALFGSLSTLEVLHAAASLHV 84
           L+TA +S        A+ P      AV P  P VDF + +  + S  T E+  +   L +
Sbjct: 20  LSTAPASREQPAAGPAAVPGGGSATAVRPPVPAVDFGNAQEAYRSRRTWELARSLLVLRL 79

Query: 85  AAVGPVV--DVGMWVMNSKLMDVELARDVIFGTVRHSFYRHFCAGEDDTAVGKTVRRLHN 144
            A   ++     +  ++ KL+   L   +    ++ +FY HF AGED  ++   +R    
Sbjct: 80  CAWPALLARHEQLLYVSRKLLGQRLFNKL----MKMTFYGHFVAGEDQESIQPLLRHYRA 139

Query: 145 VGLRSMLDYAIE------------------------------------------------ 204
            G+ ++LDY +E                                                
Sbjct: 140 FGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHRAFGDRRNGVI 199

Query: 205 ------YANDEESCDRNLHGFLRTIEATRALPRGSASFVVAKVSAICPLRLLERVSDLL- 264
                 YAN E  CD ++  FLR IEA+  +      F+  K++A+   + L + S++L 
Sbjct: 200 SARTYFYAN-EAKCDSHMETFLRCIEASGRV--SDDGFIAIKLTALGRPQFLLQFSEVLA 259

Query: 265 RW----------QQKDPCLNLPWKLK--------------------------------TF 324
           +W          Q +     +  KL+                                T 
Sbjct: 260 KWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTM 319

Query: 325 PILSESS------PLYHTLQQPNPLTNE---------EQKSLQISH--QRLMKICQSCVD 384
            +L  SS       L   L  PN  T +         E++ LQ++   QR+  + +   +
Sbjct: 320 DLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATE 379

Query: 385 ANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNPIVYGTLQAYLKDAKDRLLLVTKEAN 444
             V L +DAE T  QPAI   T       N +  P+++ T Q YLKDA D + L  + A 
Sbjct: 380 MGVRLMVDAEQTYFQPAISRLTLEMQRKFNVE-KPLIFNTYQCYLKDAYDNVTLDVELAR 439

Query: 445 EMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIATGSSG 488
                 G KLVRGAY++ E   A+ +G+E PI+ +   T+A Y+ C  ++L+ +   +  
Sbjct: 440 REGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKA 499

BLAST of Csor.00g312230 vs. NCBI nr
Match: KAG6578518.1 (Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 968 bits (2503), Expect = 0.0
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180

Query: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240
           RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELGRRMKEYLRGERRAF 497
           ELGRRMKEYLRGERRAF
Sbjct: 481 ELGRRMKEYLRGERRAF 497

BLAST of Csor.00g312230 vs. NCBI nr
Match: XP_022939392.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 964 bits (2491), Expect = 0.0
Identity = 494/497 (99.40%), Postives = 495/497 (99.60%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATR LP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRVLP 180

Query: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240
           RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPI SESSPLYHTLQQ
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELGRRMKEYLRGERRAF 497
           ELGRR+KEYLRGERRAF
Sbjct: 481 ELGRRVKEYLRGERRAF 497

BLAST of Csor.00g312230 vs. NCBI nr
Match: XP_023551146.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 944 bits (2441), Expect = 0.0
Identity = 488/498 (97.99%), Postives = 492/498 (98.80%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPH-LNLAVSPAAPTVD 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPP  LNLAVSPAAPTVD
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPPPLNLAVSPAAPTVD 60

Query: 61  FSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHS 120
           FSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHS
Sbjct: 61  FSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHS 120

Query: 121 FYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRAL 180
           FYRHFCAGEDDTAVGKTVRRLH+VGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRAL
Sbjct: 121 FYRHFCAGEDDTAVGKTVRRLHDVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRAL 180

Query: 181 PRGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQ 240
           P GSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPI SESSPLYHTLQ
Sbjct: 181 PPGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQ 240

Query: 241 QPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK 300
           QPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK
Sbjct: 241 QPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK 300

Query: 301 DHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESP 360
           DHNPIVYGTLQAYLKDAKDRLLLVTKEAN+MRVPLGIKLVRGAYMSSESKLASSLGFESP
Sbjct: 301 DHNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLE 420
           IHNSIHDTHACYNSCASFLLDNIA GSSGAILATHNV+SGKLAAKKAYEIGIEKLK+KLE
Sbjct: 361 IHNSIHDTHACYNSCASFLLDNIAVGSSGAILATHNVDSGKLAAKKAYEIGIEKLKEKLE 420

Query: 421 FAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480
           FAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR
Sbjct: 421 FAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480

Query: 481 KELGRRMKEYLRGERRAF 497
           KEL RRMKEYLRGERRAF
Sbjct: 481 KELRRRMKEYLRGERRAF 498

BLAST of Csor.00g312230 vs. NCBI nr
Match: XP_022993279.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 481/497 (96.78%), Postives = 484/497 (97.38%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYA NRLLNTAA     AFTSAASTPPP LNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYASNRLLNTAA-----AFTSAASTPPP-LNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARD+IFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDIIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNL GFLRTIEATRALP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLRGFLRTIEATRALP 180

Query: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240
            GSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPI SESSPLYHTLQQ
Sbjct: 181 PGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEAN+MRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIA GSSGAILATHNVESGKL AKKAYEIGIEKLK+KLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALSYGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELGRRMKEYLRGERRAF 497
           ELGRRMKEYLRGERRAF
Sbjct: 481 ELGRRMKEYLRGERRAF 491

BLAST of Csor.00g312230 vs. NCBI nr
Match: KAG7016079.1 (Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 852 bits (2202), Expect = 3.76e-310
Identity = 444/485 (91.55%), Postives = 444/485 (91.55%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG            
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG------------ 180

Query: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240
                                       WQQKDPCLNLPWKLKTFPI SESSPLYHTLQQ
Sbjct: 181 ----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 445

Query: 481 ELGRR 485
           ELGRR
Sbjct: 481 ELGRR 445

BLAST of Csor.00g312230 vs. ExPASy TrEMBL
Match: A0A6J1FGN7 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1)

HSP 1 Score: 964 bits (2491), Expect = 0.0
Identity = 494/497 (99.40%), Postives = 495/497 (99.60%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATR LP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRVLP 180

Query: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240
           RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPI SESSPLYHTLQQ
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELGRRMKEYLRGERRAF 497
           ELGRR+KEYLRGERRAF
Sbjct: 481 ELGRRVKEYLRGERRAF 497

BLAST of Csor.00g312230 vs. ExPASy TrEMBL
Match: A0A6J1JZR8 (Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1)

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 481/497 (96.78%), Postives = 484/497 (97.38%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYA NRLLNTAA     AFTSAASTPPP LNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYASNRLLNTAA-----AFTSAASTPPP-LNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARD+IFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDIIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNL GFLRTIEATRALP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLRGFLRTIEATRALP 180

Query: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240
            GSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPI SESSPLYHTLQQ
Sbjct: 181 PGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEAN+MRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIA GSSGAILATHNVESGKL AKKAYEIGIEKLK+KLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALSYGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELGRRMKEYLRGERRAF 497
           ELGRRMKEYLRGERRAF
Sbjct: 481 ELGRRMKEYLRGERRAF 491

BLAST of Csor.00g312230 vs. ExPASy TrEMBL
Match: A0A5D3BNA9 (Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002050 PE=3 SV=1)

HSP 1 Score: 806 bits (2083), Expect = 6.22e-292
Identity = 406/490 (82.86%), Postives = 448/490 (91.43%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MAR+++STQSKI K VF Y FNR LNTAASS AAAFT  A  PP HLN    P+ PTVDF
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFT--APPPPLHLN---PPSTPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           +D++ALFGS+ T ++LHA A+LH +A+GPVVDVGMWVMNSKLMDVEL RDV+ GTV+H+F
Sbjct: 61  TDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHTF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180
           YRHFCAGEDDT+V KTVRRLH+VGLRSMLDYA+EYA+DE SCDRNL GFLRT+EAT++LP
Sbjct: 121 YRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSLP 180

Query: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240
            GSASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PI SESSPLYHTL+Q
Sbjct: 181 SGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           P PLT EE+KSLQISH+RLMKICQSCVD NVPLAIDAEHTK+QPAIDYFTYSAAIIHNKD
Sbjct: 241 PEPLTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
            NPIVYGT+QAYLKDAKDRLLL  KEA+++++PLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 RNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HN+I DTH+CYN+CASFLLD+IA GSSGAILATHNVESGKLAA +AYEIGI KLK+KLEF
Sbjct: 361 HNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELGRRMKEYL 490
           ELGRRMKEYL
Sbjct: 481 ELGRRMKEYL 485

BLAST of Csor.00g312230 vs. ExPASy TrEMBL
Match: A0A0A0KXN4 (Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1)

HSP 1 Score: 805 bits (2078), Expect = 4.49e-291
Identity = 408/494 (82.59%), Postives = 453/494 (91.70%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSA-ASTPPP---HLNLAVSPAAP 60
           MA++I+STQSKI K VFFY FNR LNTAASS AAAFT+A A+T PP   HLN    P+ P
Sbjct: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLN---PPSTP 60

Query: 61  TVDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTV 120
           TVDF+D++ALFGS+ T ++LHA A+LH AAVGPVVDVGMWVMNSKLMDVEL RDV+ GTV
Sbjct: 61  TVDFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTV 120

Query: 121 RHSFYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEAT 180
           +HSFYRHFCAGEDDT+V KTVRRLH+VGLRSMLDYA+EYA+DE SCDRNL GFL T+EAT
Sbjct: 121 KHSFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEAT 180

Query: 181 RALPRGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYH 240
           ++LP GSASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PI SESSPLYH
Sbjct: 181 KSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYH 240

Query: 241 TLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAII 300
           TL+QP PLT EE+KSLQ+SH+RLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAII
Sbjct: 241 TLEQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAII 300

Query: 301 HNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGF 360
           HNKD NPIVYGT+QAYLKDAKDRLLL  KEA++++VPLGIKLVRGAYMSSESKLASSLGF
Sbjct: 301 HNKDRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGF 360

Query: 361 ESPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKK 420
           ESPIH++I DTH+CYN+CASFLLD+IA GS+GAILATHNVESGKLAA +AYEIGI KLK+
Sbjct: 361 ESPIHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQ 420

Query: 421 KLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQ 480
           KLEFAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDR+
Sbjct: 421 KLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRE 480

Query: 481 LMRKELGRRMKEYL 490
           LMRKELGRRMKEY+
Sbjct: 481 LMRKELGRRMKEYI 491

BLAST of Csor.00g312230 vs. ExPASy TrEMBL
Match: A0A1S3CBE9 (Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1)

HSP 1 Score: 795 bits (2054), Expect = 1.45e-286
Identity = 402/497 (80.89%), Postives = 449/497 (90.34%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MAR+++STQSKI K VF Y FNR LNTAASS AAAFT  A  PP HLN    P+ PTVDF
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFT--APPPPLHLN---PPSTPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           +D++ALFGS+ T ++LHA A+LH +A+GPVVDVGMWVMNSKLMDVEL RDV+ GTV+H+F
Sbjct: 61  TDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHTF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180
           YRHFCAGEDDT+V KTVRRLH+VGLRSMLDYA+EYA+DE SCDRNL GFLRT+EAT++LP
Sbjct: 121 YRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSLP 180

Query: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240
            GSASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PI SESSPLYHTL+Q
Sbjct: 181 SGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           P PLT EE+KSLQISH+RLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHNKD
Sbjct: 241 PEPLTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
            NPIVYGT+QAYLKDAKDRLLL  KEA+++++PLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 RNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HN+I DTH+CYN+CASFLLD+IA GSSGAILATHNVESGKLAA +AYEIGI KLK+KLEF
Sbjct: 361 HNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480
           AQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELGRRMKEYLRGERRAF 497
           E G++ +  L+ +  AF
Sbjct: 481 EFGKKDERVLKLKEIAF 492

BLAST of Csor.00g312230 vs. TAIR 10
Match: AT3G30775.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 494.2 bits (1271), Expect = 1.2e-139
Identity = 249/464 (53.66%), Postives = 331/464 (71.34%), Query Frame = 0

Query: 26  NTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDFSDTKALFGSLSTLEVLHAAASLHVA 85
           +TA      +F   A  PP H       +   +D SD   LF S+ T ++L + A LH A
Sbjct: 31  STAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRSTAVLHAA 90

Query: 86  AVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSFYRHFCAGEDDTAVGKTVRRLHN-VG 145
           A+GP+VD+G WVM+SKLMD  + R ++ G V+ +FY HFCAGED  A  + VR ++   G
Sbjct: 91  AIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRSVYEATG 150

Query: 146 LRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALPRGSASFVVAKVSAICPLRLLERVS 205
           L+ ML Y +E+A+D  SCD N+  F+RTIEA ++LP    S VV K++AICP+ LL+RVS
Sbjct: 151 LKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPISLLKRVS 210

Query: 206 DLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQ 265
           DLLRW+ K P   L WKLK+FP+ SESSPLYHT  +P PLT EE++ L+ +H R+ +IC+
Sbjct: 211 DLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGRIQEICR 270

Query: 266 SCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHN-PIVYGTLQAYLKDAKDRLLLV 325
            C ++NVPL IDAE T +QPAIDY  YS+AI+ N D + PIVY T+QAYL+DA +RL L 
Sbjct: 271 KCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAGERLHLA 330

Query: 326 TKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIA 385
            + A +  VP+G KLVRGAYMSSE+ LA SLG +SP+H++I DTH+CYN C +FL++  +
Sbjct: 331 VQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTFLMEKAS 390

Query: 386 TGSS-GAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVS 445
            GS  G +LATHN +SG+LA++KA ++GI+K   K+EFAQLYGMS+ALS+GL+ AGF VS
Sbjct: 391 NGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKRAGFNVS 450

Query: 446 KYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRM 487
           KYMPFG V   +PYLLRRA ENRG+++    DRQLMR EL RR+
Sbjct: 451 KYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMRMELKRRL 494

BLAST of Csor.00g312230 vs. TAIR 10
Match: AT5G38710.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 486.1 bits (1250), Expect = 3.3e-137
Identity = 238/432 (55.09%), Postives = 315/432 (72.92%), Query Frame = 0

Query: 58  VDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVR 117
           +D SD   LF S+    +L + A LH  ++GP+VD+G W+M+SKLMD  + RD++   V+
Sbjct: 42  LDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDLVLRIVK 101

Query: 118 HSFYRHFCAGEDDTAVGKTVRRLH-NVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEAT 177
            +FY HFCAGED  A  + V  ++ + GL+ ML Y +E+A D  +CD N+  F+ T+EA 
Sbjct: 102 GTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFIETVEAA 161

Query: 178 RALPRGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYH 237
           + LP    S VV K++AICP+ +L+RVSDLLRWQ K+P   LPWKL +FP+ S  SPLYH
Sbjct: 162 KTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYH 221

Query: 238 TLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAII 297
           T  +P PLT EE++ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  Y +AI+
Sbjct: 222 TTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIM 281

Query: 298 HNKDHN-PIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLG 357
            N D + PIVY T+QAYLKDA +RL L  +E+ +M VP+G KLVRGAYMSSE+KLA SLG
Sbjct: 282 FNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLG 341

Query: 358 FESPIHNSIHDTHACYNSCASFLLDNIATGSS-GAILATHNVESGKLAAKKAYEIGIEKL 417
           ++SP+H++I +TH CYN C SFL++  + GS    ILATHN +SGKL A+KA E+GI K 
Sbjct: 342 YKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKE 401

Query: 418 KKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLD 477
             K+EFAQLYGMS+ALS+GL+ AGF VSKYMP+G VD  +PYL+RRA ENRG++S   LD
Sbjct: 402 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALD 461

Query: 478 RQLMRKELGRRM 487
           RQLMRKEL RR+
Sbjct: 462 RQLMRKELKRRV 473

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P929831.7e-13853.66Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 P... [more]
Q6NKX14.7e-13655.09Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 P... [more]
Q86H281.7e-4832.43Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=p... [more]
A6QQ742.7e-3830.70Hydroxyproline dehydrogenase OS=Bos taurus OX=9913 GN=PRODH2 PE=2 SV=1[more]
O432727.7e-3826.03Proline dehydrogenase 1, mitochondrial OS=Homo sapiens OX=9606 GN=PRODH PE=1 SV=... [more]
Match NameE-valueIdentityDescription
KAG6578518.10.0100.00Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022939392.10.099.40proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata][more]
XP_023551146.10.097.99proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_022993279.10.096.78proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima][more]
KAG7016079.13.76e-31091.55Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. a... [more]
Match NameE-valueIdentityDescription
A0A6J1FGN70.099.40Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1[more]
A0A6J1JZR80.096.78Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1[more]
A0A5D3BNA96.22e-29282.86Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16... [more]
A0A0A0KXN44.49e-29182.59Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1[more]
A0A1S3CBE91.45e-28680.89Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G30775.11.2e-13953.66Methylenetetrahydrofolate reductase family protein [more]
AT5G38710.13.3e-13755.09Methylenetetrahydrofolate reductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.220coord: 100..492
e-value: 2.6E-90
score: 305.3
NoneNo IPR availablePANTHERPTHR13914:SF25PROLINE DEHYDROGENASEcoord: 10..488
IPR002872Proline dehydrogenase domainPFAMPF01619Pro_dhcoord: 135..469
e-value: 8.8E-74
score: 248.5
IPR015659Proline oxidase familyPANTHERPTHR13914PROLINE OXIDASEcoord: 10..488
IPR029041FAD-linked oxidoreductase-likeSUPERFAMILY51730FAD-linked oxidoreductasecoord: 127..489

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g312230.m01Csor.00g312230.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010133 proline catabolic process to glutamate
biological_process GO:0006562 proline catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0071949 FAD binding
molecular_function GO:0004657 proline dehydrogenase activity