Csor.00g310220 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g310220
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioncytochrome P450 86A8
LocationCsor_Chr17: 2657182 .. 2658836 (-)
RNA-Seq ExpressionCsor.00g310220
SyntenyCsor.00g310220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGTGGGTTTGGGTTTGGTTATTGTAATGGCGGTGACGGCGTATCTTCTGTGGTTTACGTTCATTTCCCGGTCGTTGAAAGGGCCTCAAATGTGGCCGTTGCTGGGCAGCTTGCCGGGGTTGATCGAGAATTGCGACCGTTTACACGACTGGATCTCCGACAACCTCCGTGCTTGCGGCGGCACTTATCAAACATGCATTTGGGCCTTCCCTTTCTTCGCTACAAAACAGGGTCTCGTGACTGTCACGTGCGACCCGAGAAATCTGGAGCATATACTCAAGACCCGATTCGATAATTACCCTAAGGGCCCCACTTGGCAAGCTGTATTTCACGAGTTACTTGGCCAAGGTATTTTTAATTCTGATGGTGACACGTGGCTCTTCCAGAGAAAAACTGCTGCCTTGGAATTTACTACTAGGACCCTTCGTCAAGCCATGGCTCGATGGGTTAGCCGAGCTATTAAGTTGAGGTTTTGTCCTATTCTTAAACAAGCCGAGCTTAATTCTCAGCCGATTGATCTTCAGGATGTGTTGTTGCGGCTTACTTTTGATAATATTTGCGGCTTGGCTTTTGGGAAAGATCCTATGACTTGCGCGCCGGGTTTCCCTGAAAATGGCTTTGCTATGGCTTTTGATCGAGCCACTGAAGCTTCGTTGCAACGGTTTATTTTTCCGGAGGTTCTTTGGAAATTTAAAAAGTGGCTTCGGCTTGGTATGGAAGTCAGCTTGAGCCGGAGTCTCGTTCATATCGAGGAGTATTTATCCGGTGTTATTGATTCACGTAAGCTCGAGTTGCTGAATCAGCTTAAAGACTCGACCCCACATGATGATTTATTGTCACGTTTCATGAAGAAGAAGGAAAGTAATTACTCCAACGCGTTTCTTCGCCATGTGGCGCTTAATTTCATCCTAGCTGGACGGGACACGTCATCCGTTGCTCTGAGCTGGTTCTTCTGGTTGGTGACATTAAACCCACAAATTGAAGAAAAAATCCTCCGCGAGATCTGCACCGTTCTGATTGAGACACGTGGCAATAATATCAATCATGGCTGGATGAGCCGTTAGGATTTGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTTAGACTGTACCCGTCGGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACATTCTTCCCGACGGAACATTCGTTCCGGCGGGATCGTCGGTGACTTATTCCATCTATTCCACCGGAAGAATGAAGTCGACGTGGGGAGAAGACTGCCTCGAATTCCGGCCGGAGCGGTGGCTGACGGCGGACGGGAAGAAATTCATACCATACGATCCGTACAAATTCGTAGCGTTCAATGCAGGGCCAAGGATCTGCTTGGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATAGCGGCGGCGGTACTTCTCCGACACCGGCTGACGGTGGCGGCAGGGCATAAGGTGGAGCAGAAAATGTCGTTGACGCTGTTTATGAAGTACGGGCTGAAGGTGAACGTTCACCGGAGGGAGTTGGAGGGAATAGTGGAAAGCATAAGGGGCAAAAACGGAAATTCACAAGGGAATGATACTCCGTTAGTCGCCGTTGTGAACTGTAACGGCGGGTGTAACGGTTTTAACGACGTACAACGCTAA

mRNA sequence

ATGGACGTGGGTTTGGGTTTGGTTATTGTAATGGCGGTGACGGCGTATCTTCTGTGGTTTACGTTCATTTCCCGGTCGTTGAAAGGGCCTCAAATGTGGCCGTTGCTGGGCAGCTTGCCGGGGTTGATCGAGAATTGCGACCGTTTACACGACTGGATCTCCGACAACCTCCGTGCTTGCGGCGGCACTTATCAAACATGCATTTGGGCCTTCCCTTTCTTCGCTACAAAACAGGGTCTCGTGACTGTCACGTGCGACCCGAGAAATCTGGAGCATATACTCAAGACCCGATTCGATAATTACCCTAAGGGCCCCACTTGGCAAGCTGTATTTCACGAGTTACTTGGCCAAGGTATTTTTAATTCTGATGGTGACACGTGGCTCTTCCAGAGAAAAACTGCTGCCTTGGAATTTACTACTAGGACCCTTCGTCAAGCCATGGCTCGATGGGTTAGCCGAGCTATTAAGTTGAGGTTTTGTCCTATTCTTAAACAAGCCGAGCTTAATTCTCAGCCGATTGATCTTCAGGATGTGTTGTTGCGGCTTACTTTTGATAATATTTGCGGCTTGGCTTTTGGGAAAGATCCTATGACTTGCGCGCCGGGTTTCCCTGAAAATGGCTTTGCTATGGCTTTTGATCGAGCCACTGAAGCTTCGTTGCAACGGTTTATTTTTCCGGAGGTTCTTTGGAAATTTAAAAAGTGGCTTCGGCTTGGTATGGAAGTCAGCTTGAGCCGGAGTCTCGTTCATATCGAGGAGTATTTATCCGGTGTTATTGATTCACGATTTGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTTAGACTGTACCCGTCGGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACATTCTTCCCGACGGAACATTCGTTCCGGCGGGATCGTCGGTGACTTATTCCATCTATTCCACCGGAAGAATGAAGTCGACGTGGGGAGAAGACTGCCTCGAATTCCGGCCGGAGCGGTGGCTGACGGCGGACGGGAAGAAATTCATACCATACGATCCGTACAAATTCGTAGCGTTCAATGCAGGGCCAAGGATCTGCTTGGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATAGCGGCGGCGGTACTTCTCCGACACCGGCTGACGGTGGCGGCAGGGCATAAGGTGGAGCAGAAAATGTCGTTGACGCTGTTTATGAAGTACGGGCTGAAGGTGAACGTTCACCGGAGGGAGTTGGAGGGAATAGTGGAAAGCATAAGGGGCAAAAACGGAAATTCACAAGGGAATGATACTCCGTTAGTCGCCGTTGTGAACTGTAACGGCGGGTGTAACGGTTTTAACGACGTACAACGCTAA

Coding sequence (CDS)

ATGGACGTGGGTTTGGGTTTGGTTATTGTAATGGCGGTGACGGCGTATCTTCTGTGGTTTACGTTCATTTCCCGGTCGTTGAAAGGGCCTCAAATGTGGCCGTTGCTGGGCAGCTTGCCGGGGTTGATCGAGAATTGCGACCGTTTACACGACTGGATCTCCGACAACCTCCGTGCTTGCGGCGGCACTTATCAAACATGCATTTGGGCCTTCCCTTTCTTCGCTACAAAACAGGGTCTCGTGACTGTCACGTGCGACCCGAGAAATCTGGAGCATATACTCAAGACCCGATTCGATAATTACCCTAAGGGCCCCACTTGGCAAGCTGTATTTCACGAGTTACTTGGCCAAGGTATTTTTAATTCTGATGGTGACACGTGGCTCTTCCAGAGAAAAACTGCTGCCTTGGAATTTACTACTAGGACCCTTCGTCAAGCCATGGCTCGATGGGTTAGCCGAGCTATTAAGTTGAGGTTTTGTCCTATTCTTAAACAAGCCGAGCTTAATTCTCAGCCGATTGATCTTCAGGATGTGTTGTTGCGGCTTACTTTTGATAATATTTGCGGCTTGGCTTTTGGGAAAGATCCTATGACTTGCGCGCCGGGTTTCCCTGAAAATGGCTTTGCTATGGCTTTTGATCGAGCCACTGAAGCTTCGTTGCAACGGTTTATTTTTCCGGAGGTTCTTTGGAAATTTAAAAAGTGGCTTCGGCTTGGTATGGAAGTCAGCTTGAGCCGGAGTCTCGTTCATATCGAGGAGTATTTATCCGGTGTTATTGATTCACGATTTGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTTAGACTGTACCCGTCGGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACATTCTTCCCGACGGAACATTCGTTCCGGCGGGATCGTCGGTGACTTATTCCATCTATTCCACCGGAAGAATGAAGTCGACGTGGGGAGAAGACTGCCTCGAATTCCGGCCGGAGCGGTGGCTGACGGCGGACGGGAAGAAATTCATACCATACGATCCGTACAAATTCGTAGCGTTCAATGCAGGGCCAAGGATCTGCTTGGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATAGCGGCGGCGGTACTTCTCCGACACCGGCTGACGGTGGCGGCAGGGCATAAGGTGGAGCAGAAAATGTCGTTGACGCTGTTTATGAAGTACGGGCTGAAGGTGAACGTTCACCGGAGGGAGTTGGAGGGAATAGTGGAAAGCATAAGGGGCAAAAACGGAAATTCACAAGGGAATGATACTCCGTTAGTCGCCGTTGTGAACTGTAACGGCGGGTGTAACGGTTTTAACGACGTACAACGCTAA

Protein sequence

MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRACGGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLLRLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGMEVSLSRSLVHIEEYLSGVIDSRFEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTWGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVESIRGKNGNSQGNDTPLVAVVNCNGGCNGFNDVQR
Homology
BLAST of Csor.00g310220 vs. ExPASy Swiss-Prot
Match: O80823 (Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 5.7e-197
Identity = 343/525 (65.33%), Postives = 383/525 (72.95%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++   L+I+ A+TAY LW TFISR LKGP++WP+LGSLPGLIENC+R+HDWISDNLRAC
Sbjct: 1   MEISTALMILSAITAYFLWLTFISRCLKGPRVWPILGSLPGLIENCERMHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
            GTYQTCI A PF A KQGLVTVTCDPRNLEHILK RFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  SGTYQTCICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWV+RAIKLRF PIL+ A L S+PIDLQD+LL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQDLLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP TCAPG P N FA+AFDRATEASLQRFI PE+LWKFK+WLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSL+RSLV ++ YLS +I +R                                      
Sbjct: 241 EVSLTRSLVQVDNYLSEIITTRKEEMMTQHNNGKHHDDLLSRFIKKKESYSDETLQRVAL 300

Query: 301 --------------------------------------------------------FEEI 360
                                                                    EE+
Sbjct: 301 NFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTVLVETRGDDVALWTDEPLSCEEL 360

Query: 361 DRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTWG 420
           DRL++LKAAL+ETLRLYPSVPEDSK  V DD+LPDGTFVPAGSS+TYSIYS GRMKSTWG
Sbjct: 361 DRLVFLKAALSETLRLYPSVPEDSKRAVKDDVLPDGTFVPAGSSITYSIYSAGRMKSTWG 420

Query: 421 EDCLEFRPERWLT-ADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 431
           EDCLEF+PERW++ +DG +FI +DP+KFVAFNAGPRICLGKDLAYLQMKSIA+AVLLRHR
Sbjct: 421 EDCLEFKPERWISQSDGGRFINHDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLRHR 480

BLAST of Csor.00g310220 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.1e-192
Identity = 344/546 (63.00%), Postives = 387/546 (70.88%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDV   +++V  V AY LWF  ISR LKGP++WP+LGSLPGLIE  DR+HDWI++NLRAC
Sbjct: 1   MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI A PF A KQGLVTVTCDP+N+EH+LKTRFDNYPKGPTWQAVFH+ LGQGIF
Sbjct: 61  GGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL+ A+ N +P+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA AFDRATEASLQRFI PE LW+ KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSL  I+ YL  VI++R                                      
Sbjct: 241 EVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQSYSETFLRHV 300

Query: 301 ----------------------------------------------------------FE 360
                                                                     F+
Sbjct: 301 ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD 360

Query: 361 EIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKST 420
           E+DRL+YLKAAL+ETLRLYPSVPEDSKHVV DDILPDGTFVPAGSSVTYSIY+ GRMKST
Sbjct: 361 EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST 420

Query: 421 WGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRH 451
           WGEDCLEF+PERW++ D  KF+ +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAAVLLRH
Sbjct: 421 WGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRH 480

BLAST of Csor.00g310220 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.0e-189
Identity = 336/548 (61.31%), Postives = 386/548 (70.44%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++   +++V  VT Y LWF  ISR LKGP++WPLLGSLPGLIE  DR+H+WI++NLRAC
Sbjct: 1   MEISNAMLLVAIVTGYWLWFKRISRWLKGPRVWPLLGSLPGLIEQRDRMHEWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI+A PF A KQGLVTVTCDP+NLEH+LKTRFDNYPKGPTWQ+VFH+LLGQGIF
Sbjct: 61  GGTYQTCIFAVPFLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL  A+ N++P+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA AFDRATEASLQRFI P+ +WK KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSL  I+EYL+ VI++R                                      
Sbjct: 241 EVSLSRSLGEIDEYLAAVINTRKQELMSQQESGTHQRHDDLLSRFMMKKTESYSDTFLQH 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 VALNFILAGRDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLG 360

Query: 361 FEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMK 420
           F+EIDRL+YLKAA++ETLRLYPSVPEDSKHV  DD+LPDGTFVPAGSSVTYSIY+ GRMK
Sbjct: 361 FDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMK 420

Query: 421 STWGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLL 451
           STWGEDCLEF PERW++    KFI +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAAVLL
Sbjct: 421 STWGEDCLEFNPERWISPIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLL 480

BLAST of Csor.00g310220 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 6.6e-177
Identity = 316/521 (60.65%), Postives = 367/521 (70.44%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQ---MWPLLGSLPGLIENCDRLHDWISDNL 60
           M+V   ++IV  V AYLLWF  I++S+KGP+   MWP++GSLPGLIEN +R+H+WI+DNL
Sbjct: 1   MEVSTTMMIVAIVAAYLLWFKSITKSMKGPKGPTMWPVVGSLPGLIENGNRMHEWIADNL 60

Query: 61  RACGGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQ 120
           RAC GTYQTCI A PF A KQ LVTVTCDP+NLEHILK RFDNYPKGPTWQAVFH+LLG+
Sbjct: 61  RACSGTYQTCICAIPFLARKQ-LVTVTCDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGE 120

Query: 121 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQD 180
           GIFNSDGDTWLFQRKTAALEFTTRTLRQAM RWV+RAIK RFCPIL+ A++  +P+DLQD
Sbjct: 121 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRAIKNRFCPILEMAQVQGKPVDLQD 180

Query: 181 VLLRLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLR 240
           +LLRLTFDNICGLAFGKDP T +P  PEN FA +FDRATEA+L RFI PE +WK KK L 
Sbjct: 181 LLLRLTFDNICGLAFGKDPETLSPELPENNFATSFDRATEATLHRFIMPEFVWKLKKMLG 240

Query: 241 LGMEVSLSRSLVHIEEYLSGVIDSR----------------------------------- 300
           LG+EVSL+ SL  ++ Y++ VI++R                                   
Sbjct: 241 LGLEVSLNNSLKQVDNYMTDVINTRKLELLNHQNGGPQHDDLLSRFMKKKESYSDKFLQH 300

Query: 301 -----------------------------------------------------------F 360
                                                                      F
Sbjct: 301 VALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVEICTILAETRGNDTSKWLEEPLVF 360

Query: 361 EEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKS 420
           EE+D+L+YLKAAL+ETLRLYPSVPEDSKHV++DD LPDGTFVPAGS++TYSIYSTGRMK 
Sbjct: 361 EEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGRMKF 420

Query: 421 TWGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLR 425
            WGEDCLEF+PERW++ DG KF   D ++FVAFNAGPRICLGKDLAYLQMKSIAAAVLLR
Sbjct: 421 IWGEDCLEFKPERWMSQDGDKFQVQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAVLLR 480

BLAST of Csor.00g310220 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 2.6e-173
Identity = 305/518 (58.88%), Postives = 359/518 (69.31%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MD     +I+  +  Y++WF  + RS KGP++WPL+GSLP LI N  R+HD+I+DNLR C
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI+  PF A KQG VTVTCDP+NLEHILKTRFDNYPKGP+WQ+VFH+LLG GIF
Sbjct: 61  GGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTW FQRKTAALEFTTRTLRQAMARWV RAIK R  PIL+ A   ++PIDLQDVLL
Sbjct: 121 NSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP T +P FPENGFA+AFD ATEA+LQRFI PE +WK +KWLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           E  +SRS+ H++ YLS +I++R                                      
Sbjct: 241 EDDMSRSISHVDNYLSEIINTRKLELLGQQQDESRHDDLLSRFMKKKESYSDKYLKYVAL 300

Query: 301 --------------------------------------------------------FEEI 360
                                                                   F+EI
Sbjct: 301 NFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFDEI 360

Query: 361 DRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTWG 420
           D+L+YLKAAL+ETLRLYPSVPEDSK VVA+D+LPDGTFVP+GS+VTYSIYS GRMK  WG
Sbjct: 361 DQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIWG 420

Query: 421 EDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHRL 425
           EDCLEF+PERWL  +  +    + YKFVAFNAGPRICLGKDLAYLQMKSI A++LLRHRL
Sbjct: 421 EDCLEFKPERWL--EESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRHRL 480

BLAST of Csor.00g310220 vs. NCBI nr
Match: KAG6575179.1 (Cytochrome P450 86A8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSRFEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILP 300
           EVSLSRSLVHIEEYLSGVIDSRFEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILP
Sbjct: 241 EVSLSRSLVHIEEYLSGVIDSRFEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILP 300

Query: 301 DGTFVPAGSSVTYSIYSTGRMKSTWGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGP 360
           DGTFVPAGSSVTYSIYSTGRMKSTWGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGP
Sbjct: 301 DGTFVPAGSSVTYSIYSTGRMKSTWGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGP 360

Query: 361 RICLGKDLAYLQMKSIAAAVLLRHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGI 420
           RICLGKDLAYLQMKSIAAAVLLRHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGI
Sbjct: 361 RICLGKDLAYLQMKSIAAAVLLRHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGI 420

Query: 421 VESIRGKNGNSQGNDTPLVAVVNCNGGCNGFNDVQR 456
           VESIRGKNGNSQGNDTPLVAVVNCNGGCNGFNDVQR
Sbjct: 421 VESIRGKNGNSQGNDTPLVAVVNCNGGCNGFNDVQR 456

BLAST of Csor.00g310220 vs. NCBI nr
Match: XP_022958814.1 (cytochrome P450 86A8 [Cucurbita moschata])

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 454/551 (82.40%), Postives = 454/551 (82.40%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSLVHIEEYLS VIDSR                                      
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 456
           GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

BLAST of Csor.00g310220 vs. NCBI nr
Match: XP_023547593.1 (cytochrome P450 86A8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 886 bits (2290), Expect = 0.0
Identity = 452/551 (82.03%), Postives = 452/551 (82.03%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGL LVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSLVHIEEYLS VIDSR                                      
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 456
           GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

BLAST of Csor.00g310220 vs. NCBI nr
Match: XP_023006552.1 (cytochrome P450 86A8 [Cucurbita maxima])

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 449/551 (81.49%), Postives = 451/551 (81.85%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGL LVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFP+FATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPYFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSLVHIEEYLS VIDSR                                      
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 456
           GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

BLAST of Csor.00g310220 vs. NCBI nr
Match: XP_038907189.1 (cytochrome P450 86A8-like [Benincasa hispida])

HSP 1 Score: 860 bits (2222), Expect = 4.41e-312
Identity = 439/551 (79.67%), Postives = 443/551 (80.40%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG  LVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 23  MDVGFALVIVAAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 82

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 83  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 142

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAE NS+PIDLQDVLL
Sbjct: 143 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAEFNSEPIDLQDVLL 202

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 203 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 262

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSL RSLVHIEEYLS VIDSR                                      
Sbjct: 263 EVSLGRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 322

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 323 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLAFEE 382

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 383 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 442

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 456
           GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 443 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 502

BLAST of Csor.00g310220 vs. ExPASy TrEMBL
Match: A0A6J1H676 (cytochrome P450 86A8 OS=Cucurbita moschata OX=3662 GN=LOC111459971 PE=3 SV=1)

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 454/551 (82.40%), Postives = 454/551 (82.40%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSLVHIEEYLS VIDSR                                      
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 456
           GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

BLAST of Csor.00g310220 vs. ExPASy TrEMBL
Match: A0A6J1KW55 (cytochrome P450 86A8 OS=Cucurbita maxima OX=3661 GN=LOC111499241 PE=3 SV=1)

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 449/551 (81.49%), Postives = 451/551 (81.85%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGL LVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFP+FATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPYFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSLVHIEEYLS VIDSR                                      
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 456
           GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

BLAST of Csor.00g310220 vs. ExPASy TrEMBL
Match: A0A5D3BVJ6 (Cytochrome P450 86A8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001130 PE=3 SV=1)

HSP 1 Score: 852 bits (2200), Expect = 2.11e-309
Identity = 433/551 (78.58%), Postives = 441/551 (80.04%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG  LVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AELNS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSLVHIEEYLS VIDSR                                      
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLLETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 456
           GEDCLEFRPERWLTADGKKFI YDPYKFV+FNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIQYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

BLAST of Csor.00g310220 vs. ExPASy TrEMBL
Match: A0A1S3C7C5 (cytochrome P450 86A8-like OS=Cucumis melo OX=3656 GN=LOC103497711 PE=3 SV=1)

HSP 1 Score: 852 bits (2200), Expect = 2.11e-309
Identity = 433/551 (78.58%), Postives = 441/551 (80.04%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG  LVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AELNS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSLVHIEEYLS VIDSR                                      
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLLETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 456
           GEDCLEFRPERWLTADGKKFI YDPYKFV+FNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIQYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

BLAST of Csor.00g310220 vs. ExPASy TrEMBL
Match: A0A0A0K9Z1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004550 PE=3 SV=1)

HSP 1 Score: 847 bits (2187), Expect = 1.73e-307
Identity = 430/546 (78.75%), Postives = 437/546 (80.04%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG  LVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AELNS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFI PEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSLVHIEEYLS VIDSR                                      
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 ---------------------------------------------------------FEE 360
                                                                    FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLDEPLEFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 451
           GEDCLEFRPERWLTADG KFI YDPYKFV+FNAGPRICLGKDLAYLQMKSIAAAVLLRHR
Sbjct: 421 GEDCLEFRPERWLTADGTKFIAYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

BLAST of Csor.00g310220 vs. TAIR 10
Match: AT2G45970.1 (cytochrome P450, family 86, subfamily A, polypeptide 8 )

HSP 1 Score: 688.3 bits (1775), Expect = 4.1e-198
Identity = 343/525 (65.33%), Postives = 383/525 (72.95%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++   L+I+ A+TAY LW TFISR LKGP++WP+LGSLPGLIENC+R+HDWISDNLRAC
Sbjct: 1   MEISTALMILSAITAYFLWLTFISRCLKGPRVWPILGSLPGLIENCERMHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
            GTYQTCI A PF A KQGLVTVTCDPRNLEHILK RFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  SGTYQTCICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWV+RAIKLRF PIL+ A L S+PIDLQD+LL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQDLLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP TCAPG P N FA+AFDRATEASLQRFI PE+LWKFK+WLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSL+RSLV ++ YLS +I +R                                      
Sbjct: 241 EVSLTRSLVQVDNYLSEIITTRKEEMMTQHNNGKHHDDLLSRFIKKKESYSDETLQRVAL 300

Query: 301 --------------------------------------------------------FEEI 360
                                                                    EE+
Sbjct: 301 NFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTVLVETRGDDVALWTDEPLSCEEL 360

Query: 361 DRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTWG 420
           DRL++LKAAL+ETLRLYPSVPEDSK  V DD+LPDGTFVPAGSS+TYSIYS GRMKSTWG
Sbjct: 361 DRLVFLKAALSETLRLYPSVPEDSKRAVKDDVLPDGTFVPAGSSITYSIYSAGRMKSTWG 420

Query: 421 EDCLEFRPERWLT-ADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 431
           EDCLEF+PERW++ +DG +FI +DP+KFVAFNAGPRICLGKDLAYLQMKSIA+AVLLRHR
Sbjct: 421 EDCLEFKPERWISQSDGGRFINHDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLRHR 480

BLAST of Csor.00g310220 vs. TAIR 10
Match: AT4G00360.1 (cytochrome P450, family 86, subfamily A, polypeptide 2 )

HSP 1 Score: 674.1 bits (1738), Expect = 7.9e-194
Identity = 344/546 (63.00%), Postives = 387/546 (70.88%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDV   +++V  V AY LWF  ISR LKGP++WP+LGSLPGLIE  DR+HDWI++NLRAC
Sbjct: 1   MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI A PF A KQGLVTVTCDP+N+EH+LKTRFDNYPKGPTWQAVFH+ LGQGIF
Sbjct: 61  GGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL+ A+ N +P+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA AFDRATEASLQRFI PE LW+ KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSL  I+ YL  VI++R                                      
Sbjct: 241 EVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQSYSETFLRHV 300

Query: 301 ----------------------------------------------------------FE 360
                                                                     F+
Sbjct: 301 ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD 360

Query: 361 EIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKST 420
           E+DRL+YLKAAL+ETLRLYPSVPEDSKHVV DDILPDGTFVPAGSSVTYSIY+ GRMKST
Sbjct: 361 EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST 420

Query: 421 WGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRH 451
           WGEDCLEF+PERW++ D  KF+ +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAAVLLRH
Sbjct: 421 WGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRH 480

BLAST of Csor.00g310220 vs. TAIR 10
Match: AT1G01600.1 (cytochrome P450, family 86, subfamily A, polypeptide 4 )

HSP 1 Score: 663.3 bits (1710), Expect = 1.4e-190
Identity = 336/548 (61.31%), Postives = 386/548 (70.44%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++   +++V  VT Y LWF  ISR LKGP++WPLLGSLPGLIE  DR+H+WI++NLRAC
Sbjct: 1   MEISNAMLLVAIVTGYWLWFKRISRWLKGPRVWPLLGSLPGLIEQRDRMHEWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI+A PF A KQGLVTVTCDP+NLEH+LKTRFDNYPKGPTWQ+VFH+LLGQGIF
Sbjct: 61  GGTYQTCIFAVPFLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL  A+ N++P+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA AFDRATEASLQRFI P+ +WK KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           EVSLSRSL  I+EYL+ VI++R                                      
Sbjct: 241 EVSLSRSLGEIDEYLAAVINTRKQELMSQQESGTHQRHDDLLSRFMMKKTESYSDTFLQH 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 VALNFILAGRDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLG 360

Query: 361 FEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMK 420
           F+EIDRL+YLKAA++ETLRLYPSVPEDSKHV  DD+LPDGTFVPAGSSVTYSIY+ GRMK
Sbjct: 361 FDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMK 420

Query: 421 STWGEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLL 451
           STWGEDCLEF PERW++    KFI +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAAVLL
Sbjct: 421 STWGEDCLEFNPERWISPIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLL 480

BLAST of Csor.00g310220 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 609.8 bits (1571), Expect = 1.8e-174
Identity = 305/518 (58.88%), Postives = 359/518 (69.31%), Query Frame = 0

Query: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MD     +I+  +  Y++WF  + RS KGP++WPL+GSLP LI N  R+HD+I+DNLR C
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMC 60

Query: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI+  PF A KQG VTVTCDP+NLEHILKTRFDNYPKGP+WQ+VFH+LLG GIF
Sbjct: 61  GGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180
           NSDGDTW FQRKTAALEFTTRTLRQAMARWV RAIK R  PIL+ A   ++PIDLQDVLL
Sbjct: 121 NSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP T +P FPENGFA+AFD ATEA+LQRFI PE +WK +KWLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSGVIDSR-------------------------------------- 300
           E  +SRS+ H++ YLS +I++R                                      
Sbjct: 241 EDDMSRSISHVDNYLSEIINTRKLELLGQQQDESRHDDLLSRFMKKKESYSDKYLKYVAL 300

Query: 301 --------------------------------------------------------FEEI 360
                                                                   F+EI
Sbjct: 301 NFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFDEI 360

Query: 361 DRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTWG 420
           D+L+YLKAAL+ETLRLYPSVPEDSK VVA+D+LPDGTFVP+GS+VTYSIYS GRMK  WG
Sbjct: 361 DQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIWG 420

Query: 421 EDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHRL 425
           EDCLEF+PERWL  +  +    + YKFVAFNAGPRICLGKDLAYLQMKSI A++LLRHRL
Sbjct: 421 EDCLEFKPERWL--EESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRHRL 480

BLAST of Csor.00g310220 vs. TAIR 10
Match: AT5G58860.1 (cytochrome P450, family 86, subfamily A, polypeptide 1 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.2e-152
Identity = 275/496 (55.44%), Postives = 326/496 (65.73%), Query Frame = 0

Query: 10  VMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRACGGTYQTCIW 69
           V A++ Y LWF F+SR L GP++ P +GSLP LI N  R+HDWI+DNLRA GGTYQTC  
Sbjct: 13  VAALSVYALWFYFLSRRLTGPKVLPFVGSLPYLIANRSRIHDWIADNLRATGGTYQTCTM 72

Query: 70  AFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIFNSDGDTWLF 129
             PF A  QG  TVTC P+N+EHILKTRFDNYPKGP W+A FH+LLGQGIFNSDGDTWL 
Sbjct: 73  VIPFVAKAQGFYTVTCHPKNVEHILKTRFDNYPKGPMWRAAFHDLLGQGIFNSDGDTWLM 132

Query: 130 QRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLLRLTFDNICG 189
           QRKTAALEFTTRTLRQAMARWV+  IK R   IL +A  N++P+DLQD+ LRLTFDNICG
Sbjct: 133 QRKTAALEFTTRTLRQAMARWVNGTIKNRLWLILDRAVQNNKPVDLQDLFLRLTFDNICG 192

Query: 190 LAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFIFPEVLWKFKKWLRLGMEVSLSRSLV 249
           L FGKDP T +   P+N F++AFD ATEA+L+R ++   LW+ +K + +G E  L +SL 
Sbjct: 193 LTFGKDPETLSLDLPDNPFSVAFDTATEATLKRLLYTGFLWRIQKAMGIGSEDKLKKSLE 252

Query: 250 HIEEYLSGVIDSR----------------------------------------------- 309
            +E Y++  ID+R                                               
Sbjct: 253 VVETYMNDAIDARKNSPSDDLLSRFLKKRDVNGNVLPTDVLQRIALNFVLAGRDTSSVAL 312

Query: 310 ------------------------------------------FEEIDRLIYLKAALTETL 369
                                                     F+E DRL+YLKAAL ETL
Sbjct: 313 SWFFWLVMNNREVETKIVNELSMVLKETRGNDQEKWTEEPLEFDEADRLVYLKAALAETL 372

Query: 370 RLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTWGEDCLEFRPERWLTA 416
           RLYPSVP+D K+VV DD+LPDGTFVP GS+VTYSIYS GRMK+ WGEDCLEFRPERWLTA
Sbjct: 373 RLYPSVPQDFKYVVDDDVLPDGTFVPRGSTVTYSIYSIGRMKTIWGEDCLEFRPERWLTA 432

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O808235.7e-19765.33Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1[more]
O230661.1e-19263.00Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Q9LMM12.0e-18961.31Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
B3RFJ66.6e-17760.65Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
Q9CAD62.6e-17358.88Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6575179.10.0100.00Cytochrome P450 86A8, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022958814.10.082.40cytochrome P450 86A8 [Cucurbita moschata][more]
XP_023547593.10.082.03cytochrome P450 86A8 [Cucurbita pepo subsp. pepo][more]
XP_023006552.10.081.49cytochrome P450 86A8 [Cucurbita maxima][more]
XP_038907189.14.41e-31279.67cytochrome P450 86A8-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1H6760.082.40cytochrome P450 86A8 OS=Cucurbita moschata OX=3662 GN=LOC111459971 PE=3 SV=1[more]
A0A6J1KW550.081.49cytochrome P450 86A8 OS=Cucurbita maxima OX=3661 GN=LOC111499241 PE=3 SV=1[more]
A0A5D3BVJ62.11e-30978.58Cytochrome P450 86A8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3C7C52.11e-30978.58cytochrome P450 86A8-like OS=Cucumis melo OX=3656 GN=LOC103497711 PE=3 SV=1[more]
A0A0A0K9Z11.73e-30778.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004550 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G45970.14.1e-19865.33cytochrome P450, family 86, subfamily A, polypeptide 8 [more]
AT4G00360.17.9e-19463.00cytochrome P450, family 86, subfamily A, polypeptide 2 [more]
AT1G01600.11.4e-19061.31cytochrome P450, family 86, subfamily A, polypeptide 4 [more]
AT1G63710.11.8e-17458.88cytochrome P450, family 86, subfamily A, polypeptide 7 [more]
AT5G58860.11.2e-15255.44cytochrome P450, family 86, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 274..285
score: 43.62
coord: 363..374
score: 38.77
coord: 354..363
score: 49.59
IPR001128Cytochrome P450PFAMPF00067p450coord: 262..402
e-value: 1.3E-30
score: 106.6
coord: 28..267
e-value: 7.0E-11
score: 41.5
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 320..338
score: 23.48
coord: 269..285
score: 37.1
coord: 363..381
score: 32.39
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 264..415
e-value: 2.0E-42
score: 147.4
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..263
e-value: 3.6E-30
score: 107.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 21..415
NoneNo IPR availablePANTHERPTHR24296:SF117CYTOCHROME P450 86A2coord: 7..262
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 7..262
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 263..429
NoneNo IPR availablePANTHERPTHR24296:SF117CYTOCHROME P450 86A2coord: 263..429
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 356..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g310220.m01Csor.00g310220.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen