Csor.00g309470 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g309470
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionaspartic proteinase PCS1-like
LocationCsor_Chr01: 6620260 .. 6621309 (+)
RNA-Seq ExpressionCsor.00g309470
SyntenyCsor.00g309470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglestart_codonpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACATGGTCGTAGACACTGGCAGCCAATTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGTGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAAATATCCAAGTTTTCCTATTGCGTTCCTGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTTGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAGACTTGTGACTGAGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTGGAGTACGATCTGAGCAATAAGAGAGTTGGGTTTGGTGTTGCTAAGTGTAGTGGATTGAAGGCATGA

mRNA sequence

ATGGACATGGTCGTAGACACTGGCAGCCAATTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGTGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAAATATCCAAGTTTTCCTATTGCGTTCCTGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTTGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAGACTTGTGACTGAGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTGGAGTACGATCTGAGCAATAAGAGAGTTGGGTTTGGTGTTGCTAAGTGTAGTGGATTGAAGGCATGA

Coding sequence (CDS)

ATGGACATGGTCGTAGACACTGGCAGCCAATTGTCCTGGATTCAATGCCACGGCAAAGTAAGGAGAAAATCAGTGAAGCCCATGATTAATTGGTTTGACCCTTATCTCTCCTCCTCTTTCTCTTTCCTTCCCTGTAACACCACTCTGTGCAGACCCCGAATTCCCGATTTTACCCTTCCTACTTCTTGTGACCCAACTCGCCACTGCCACTACTCCTACTTCTACGCGGATGGGACCCTGGCCGAGGGTAATCTCGTCACTGAAAAATTCACCTTCTCCAATTCCTTAACTACCCGCTCCCTCCTTCTGGGCTGTGCTACGGCCTCCACCCAAAACAGGGGTATGTTGGGAATGAATACTGGACGCCTCTCCTTCATCTCCCAGGCCAAAATATCCAAGTTTTCCTATTGCGTTCCTGATCGAACCGGGTCGGATCTAACCGGCTTGTTCTACCTTGGAGACAACCCCAACTCCGCTAAATTCAAATACGTCAACATGTTGACTTTCCCCAAAAGTCGACTCTCCCCGAATCTTGACAAGTCGGCCTACACCCTCCCGATGAAGGGGATAAGAATAGGCAACAACAAACTCAACATCTCGCCCGCCGTTTTCAAACCGGACCCATCTGGGGCCGGTCAGACCATGATCGACTCCGGCTCCGATTTGACGTATTTGGTAGATGAGGCCTACAGCAAGGTCAGAGAAGAGATGGTCAGATTAGTGGGGCCCATGATGAAGAAAGGATACGAATACGCCGCCGTCGCCGACATGTGTTTCGACGGCGCAGTGGCGGCGGCGGTGGGTCGGAGGATCGGCGACATGTGGTTCCAGTTTGAGAATGGGGTGGAGATATTGGTTGGGAAAGGGGAAGGGTTATTGACGGAAGTGGAGGAAGGGGTGAAGTGCGTGGGGATCGGACGGTCAGATAGACTTGTGACTGAGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTGGAGTACGATCTGAGCAATAAGAGAGTTGGGTTTGGTGTTGCTAAGTGTAGTGGATTGAAGGCATGA

Protein sequence

MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLPTSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA
Homology
BLAST of Csor.00g309470 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.4e-57
Identity = 134/369 (36.31%), Postives = 196/369 (53.12%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           + MV+DTGS+LSW++C+    R S    +N FDP  SSS+S +PC++  CR R  DF +P
Sbjct: 86  ISMVIDTGSELSWLRCN----RSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIP 145

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATA--------STQN 120
            SCD  + CH +  YAD + +EGNL  E F F NS    +L+ GC  +         T+ 
Sbjct: 146 ASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKT 205

Query: 121 RGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF-PK 180
            G+LGMN G LSFISQ    KFSYC+      D  G   LGD    + F ++  L + P 
Sbjct: 206 TGLLGMNRGSLSFISQMGFPKFSYCISGT--DDFPGFLLLGD----SNFTWLTPLNYTPL 265

Query: 181 SRLS---PNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDE 240
            R+S   P  D+ AYT+ + GI++    L I  +V  PD +GAGQTM+DSG+  T+L+  
Sbjct: 266 IRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGP 325

Query: 241 AYSKVREEMVRLVGPMM----KKGYEYAAVADMCF---DGAVAAAVGRRIGDMWFQFENG 300
            Y+ +R   +     ++       + +    D+C+      + + +  R+  +   FE G
Sbjct: 326 VYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFE-G 385

Query: 301 VEILVGKGEGLLTEV------EEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNK 345
            EI V  G+ LL  V       + V C   G SD +  E+ +IG+ HQQNMW+E+DL   
Sbjct: 386 AEIAV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRS 442

BLAST of Csor.00g309470 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.8e-34
Identity = 107/347 (30.84%), Postives = 162/347 (46.69%), Query Frame = 0

Query: 4   VVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLPTSC 63
           ++DTGS L W QC    +  +    I  F+P  SSSFS LPC++ LC+       L +  
Sbjct: 111 IMDTGSDLIWTQCQPCTQCFNQSTPI--FNPQGSSSFSTLPCSSQLCQ------ALSSPT 170

Query: 64  DPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCAT-----ASTQNRGMLGM 123
                C Y+Y Y DG+  +G++ TE  TF  S++  ++  GC             G++GM
Sbjct: 171 CSNNFCQYTYGYGDGSETQGSMGTETLTF-GSVSIPNITFGCGENNQGFGQGNGAGLVGM 230

Query: 124 NTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNL 183
             G LS  SQ  ++KFSYC+    GS       LG   NS      N      S++    
Sbjct: 231 GRGPLSLPSQLDVTKFSYCMTP-IGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQI---- 290

Query: 184 DKSAYTLPMKGIRIGNNKLNISPAVFKPDP-SGAGQTMIDSGSDLTYLVDEAYSKVREEM 243
             + Y + + G+ +G+ +L I P+ F  +  +G G  +IDSG+ LTY V+ AY  VR+E 
Sbjct: 291 -PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEF 350

Query: 244 VRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVE 303
           +  +   +  G   ++  D+CF    +     +I      F+ G   L    E       
Sbjct: 351 ISQINLPVVNG--SSSGFDLCFQ-TPSDPSNLQIPTFVMHFDGG--DLELPSENYFISPS 410

Query: 304 EGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKC 345
            G+ C+ +G S +     +I GN+ QQNM V YD  N  V F  A+C
Sbjct: 411 NGLICLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Csor.00g309470 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.3e-33
Identity = 109/350 (31.14%), Postives = 165/350 (47.14%), Query Frame = 0

Query: 4   VVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLPTSC 63
           ++DTGS L W QC    +  S    I  F+P  SSSFS LPC +  C+       LP+  
Sbjct: 112 IMDTGSDLIWTQCEPCTQCFSQPTPI--FNPQDSSSFSTLPCESQYCQ------DLPSET 171

Query: 64  DPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCAT-----ASTQNRGMLGM 123
                C Y+Y Y DG+  +G + TE FTF  S +  ++  GC             G++GM
Sbjct: 172 CNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFGCGEDNQGFGQGNGAGLIGM 231

Query: 124 NTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNL 183
             G LS  SQ  + +FSYC+    GS       LG   +    +     T   S L+P  
Sbjct: 232 GWGPLSLPSQLGVGQFSYCMTS-YGSSSPSTLALGSAASGVP-EGSPSTTLIHSSLNP-- 291

Query: 184 DKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMV 243
             + Y + ++GI +G + L I  + F+    G G  +IDSG+ LTYL  +AY+ V +   
Sbjct: 292 --TYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFT 351

Query: 244 RLVGPMMKKGYEYAAVADMCF----DGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLT 303
             +   +    E ++    CF    DG+       ++ ++  QF+ GV + +G+ + +L 
Sbjct: 352 DQIN--LPTVDESSSGLSTCFQQPSDGSTV-----QVPEISMQFDGGV-LNLGE-QNILI 411

Query: 304 EVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKC 345
              EGV C+ +G S +L    +I GN+ QQ   V YDL N  V F   +C
Sbjct: 412 SPAEGVICLAMGSSSQL--GISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Csor.00g309470 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-31
Identity = 102/348 (29.31%), Postives = 164/348 (47.13%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           M +V+DTGS ++WIQC            +  F+P  SS++  L C+   C        L 
Sbjct: 175 MYLVLDTGSDVNWIQCEPCADCYQQSDPV--FNPTSSSTYKSLTCSAPQCS------LLE 234

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS----TQNRGML 120
           TS   +  C Y   Y DG+   G L T+  TF NS    ++ LGC   +    T   G+L
Sbjct: 235 TSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLL 294

Query: 121 GMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSP 180
           G+  G LS  +Q K + FSYC+ DR     + L +     NS +    +  T P  R + 
Sbjct: 295 GLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDF-----NSVQLGGGD-ATAPLLR-NK 354

Query: 181 NLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREE 240
            +D + Y + + G  +G  K+ +  A+F  D SG+G  ++D G+ +T L  +AY+ +R+ 
Sbjct: 355 KID-TFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDA 414

Query: 241 MVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEV 300
            ++L    +KKG    ++ D C+D +  + V  ++  + F F  G  + +     L+   
Sbjct: 415 FLKLT-VNLKKGSSSISLFDTCYDFSSLSTV--KVPTVAFHFTGGKSLDLPAKNYLIPVD 474

Query: 301 EEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKC 345
           + G  C     +    +  +IIGNV QQ   + YDLS   +G    KC
Sbjct: 475 DSGTFCFAFAPTS---SSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Csor.00g309470 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-28
Identity = 102/356 (28.65%), Postives = 165/356 (46.35%), Query Frame = 0

Query: 3   MVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPD-FTLPT 62
           +VVDTGS+L+W+ C  + R K  + +   F    S SF  + C T  C+  + + F+L T
Sbjct: 121 VVVDTGSELTWVNCRYRARGKDNRRV---FRADESKSFKTVGCLTQTCKVDLMNLFSLTT 180

Query: 63  SCDPTRHCHYSYFYADGTLAEGNLVTEKFT--FSNSLTTR--SLLLGCATASTQNR---- 122
              P+  C Y Y YADG+ A+G    E  T   +N    R    L+GC+++ T       
Sbjct: 181 CPTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNGRMARLPGHLIGCSSSFTGQSFQGA 240

Query: 123 -GMLGMNTGRLSFISQAKI---SKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF 182
            G+LG+     SF S A     +KFSYC+ D   +     + +  +  S K  +      
Sbjct: 241 DGVLGLAFSDFSFTSTATSLYGAKFSYCLVDHLSNKNVSNYLIFGSSRSTKTAFRRTTPL 300

Query: 183 PKSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEA 242
             +R+ P      Y + + GI +G + L+I   V+  D +  G T++DSG+ LT L D A
Sbjct: 301 DLTRIPP-----FYAINVIGISLGYDMLDIPSQVW--DATSGGGTILDSGTSLTLLADAA 360

Query: 243 YSKVREEMVRLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKG 302
           Y +V   + R +  + +   E   + + CF       V  ++  + F  + G      + 
Sbjct: 361 YKQVVTGLARYLVELKRVKPEGVPI-EYCFSFTSGFNVS-KLPQLTFHLKGGARFEPHR- 420

Query: 303 EGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCS 346
           +  L +   GVKC+G   +    T  N+IGN+ QQN   E+DL    + F  + C+
Sbjct: 421 KSYLVDAAPGVKCLGFVSAGTPAT--NVIGNIMQQNYLWEFDLMASTLSFAPSACT 461

BLAST of Csor.00g309470 vs. NCBI nr
Match: KAG6607601.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714 bits (1843), Expect = 7.40e-260
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA
Sbjct: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349

BLAST of Csor.00g309470 vs. NCBI nr
Match: KAG6607573.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714 bits (1843), Expect = 1.06e-258
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 72  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 131

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 132 TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 191

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 192 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 251

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 252 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 311

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 312 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 371

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA
Sbjct: 372 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 420

BLAST of Csor.00g309470 vs. NCBI nr
Match: KAG6607571.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714 bits (1843), Expect = 1.06e-258
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 72  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 131

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 132 TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 191

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 192 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 251

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 252 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 311

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 312 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 371

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA
Sbjct: 372 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 420

BLAST of Csor.00g309470 vs. NCBI nr
Match: XP_022932084.1 (aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932086.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932444.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022933293.1 aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022933314.1 aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 714 bits (1842), Expect = 1.51e-258
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 72  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 131

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 132 TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 191

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 192 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 251

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 252 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 311

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 312 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 371

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKR+GFGVAKCSGLKA
Sbjct: 372 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of Csor.00g309470 vs. NCBI nr
Match: XP_022932085.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 714 bits (1842), Expect = 1.51e-258
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 72  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 131

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 132 TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 191

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 192 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 251

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 252 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 311

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 312 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 371

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKR+GFGVAKCSGLKA
Sbjct: 372 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of Csor.00g309470 vs. ExPASy TrEMBL
Match: A0A6J1EWZ6 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438863 PE=3 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 7.31e-259
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 72  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 131

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 132 TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 191

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 192 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 251

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 252 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 311

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 312 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 371

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKR+GFGVAKCSGLKA
Sbjct: 372 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of Csor.00g309470 vs. ExPASy TrEMBL
Match: A0A6J1F1P3 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438856 PE=3 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 7.31e-259
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 72  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 131

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 132 TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 191

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 192 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 251

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 252 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 311

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 312 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 371

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKR+GFGVAKCSGLKA
Sbjct: 372 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of Csor.00g309470 vs. ExPASy TrEMBL
Match: A0A6J1F181 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438406 PE=3 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 7.31e-259
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 72  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 131

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 132 TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 191

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 192 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 251

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 252 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 311

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 312 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 371

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKR+GFGVAKCSGLKA
Sbjct: 372 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of Csor.00g309470 vs. ExPASy TrEMBL
Match: A0A6J1F0M7 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438404 PE=3 SV=1)

HSP 1 Score: 713 bits (1841), Expect = 1.04e-258
Identity = 347/349 (99.43%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 72  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 131

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 132 TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 191

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 192 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 251

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPA+FKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 252 SAYTLPMKGIRIGNNKLNISPAIFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 311

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 312 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 371

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKR+GFGVAKCSGLKA
Sbjct: 372 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRIGFGVAKCSGLKA 420

BLAST of Csor.00g309470 vs. ExPASy TrEMBL
Match: A0A6J1F166 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438397 PE=3 SV=1)

HSP 1 Score: 711 bits (1836), Expect = 1.20e-258
Identity = 347/349 (99.43%), Postives = 348/349 (99.71%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP
Sbjct: 29  MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 88

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 120
           TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT
Sbjct: 89  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNT 148

Query: 121 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 180
           GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK
Sbjct: 149 GRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLDK 208

Query: 181 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 240
           SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL
Sbjct: 209 SAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVRL 268

Query: 241 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 300
           VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV
Sbjct: 269 VGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEGV 328

Query: 301 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGLKA 349
           KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDL NKR+GFGVAKCSGLKA
Sbjct: 329 KCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLINKRIGFGVAKCSGLKA 377

BLAST of Csor.00g309470 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 463.0 bits (1190), Expect = 2.1e-130
Identity = 227/346 (65.61%), Postives = 269/346 (77.75%), Query Frame = 0

Query: 2   DMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLPT 61
           ++V+DTGSQLSWIQCH K  +K + P    FDP LSSSFS LPC+  LC+PRIPDFTLPT
Sbjct: 94  ELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFDPSLSSSFSDLPCSHPLCKPRIPDFTLPT 153

Query: 62  SCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNTG 121
           SCD  R CHYSYFYADGT AEGNLV EKFTFSNS TT  L+LGCA  ST  +G+LGMN G
Sbjct: 154 SCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSNSQTTPPLILGCAKESTDEKGILGMNLG 213

Query: 122 RLSFISQAKISKFSYCVP---DRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNL 181
           RLSFISQAKISKFSYC+P   +R G   TG FYLGDNPNS  FKYV++LTFP+S+  PNL
Sbjct: 214 RLSFISQAKISKFSYCIPTRSNRPGLASTGSFYLGDNPNSRGFKYVSLLTFPQSQRMPNL 273

Query: 182 DKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMV 241
           D  AYT+P++GIRIG  +LNI  +VF+PD  G+GQTM+DSGS+ T+LVD AY KV+EE+V
Sbjct: 274 DPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIV 333

Query: 242 RLVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEE 301
           RLVG  +KKGY Y + ADMCFDG  +  +GR IGD+ F+F  GVEILV K + LL  V  
Sbjct: 334 RLVGSRLKKGYVYGSTADMCFDGNHSMEIGRLIGDLVFEFGRGVEILVEK-QSLLVNVGG 393

Query: 302 GVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKC 345
           G+ CVGIGRS  L   SNIIGNVHQQN+WVE+D++N+RVGF  A+C
Sbjct: 394 GIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAEC 438

BLAST of Csor.00g309470 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 439.9 bits (1130), Expect = 1.9e-123
Identity = 226/346 (65.32%), Postives = 262/346 (75.72%), Query Frame = 0

Query: 3   MVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLPTS 62
           MV+DTGSQLSWIQCH K  +   KP  + FDP LSSSFS LPC+  LC+PRIPDFTLPTS
Sbjct: 87  MVLDTGSQLSWIQCHRK--KLPPKPKTS-FDPSLSSSFSTLPCSHPLCKPRIPDFTLPTS 146

Query: 63  CDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATASTQNRGMLGMNTGR 122
           CD  R CHYSYFYADGT AEGNLV EK TFSN+  T  L+LGCAT S+ +RG+LGMN GR
Sbjct: 147 CDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEITPPLILGCATESSDDRGILGMNRGR 206

Query: 123 LSFISQAKISKFSYCVP---DRTGSDLTGLFYLGDNPNSAKFKYVNMLTFPKSRLSPNLD 182
           LSF+SQAKISKFSYC+P   +R G   TG FYLGDNPNS  FKYV++LTFP+S+  PNLD
Sbjct: 207 LSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYLGDNPNSHGFKYVSLLTFPESQRMPNLD 266

Query: 183 KSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSKVREEMVR 242
             AYT+PM GIR G  KLNIS +VF+PD  G+GQTM+DSGS+ T+LVD AY KVR E++ 
Sbjct: 267 PLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMT 326

Query: 243 LVGPMMKKGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVEILVGKGEGLLTEVEEG 302
            VG  +KKGY Y   ADMCFDG V A + R IGD+ F F  GVEILV K E +L  V  G
Sbjct: 327 RVGRRLKKGYVYGGTADMCFDGNV-AMIPRLIGDLVFVFTRGVEILVPK-ERVLVNVGGG 386

Query: 303 VKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCS 346
           + CVGIGRS  L   SNIIGNVHQQN+WVE+D++N+RVGF  A CS
Sbjct: 387 IHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADCS 427

BLAST of Csor.00g309470 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 224.9 bits (572), Expect = 9.8e-59
Identity = 134/369 (36.31%), Postives = 196/369 (53.12%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           + MV+DTGS+LSW++C+    R S    +N FDP  SSS+S +PC++  CR R  DF +P
Sbjct: 86  ISMVIDTGSELSWLRCN----RSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIP 145

Query: 61  TSCDPTRHCHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATA--------STQN 120
            SCD  + CH +  YAD + +EGNL  E F F NS    +L+ GC  +         T+ 
Sbjct: 146 ASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKT 205

Query: 121 RGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTF-PK 180
            G+LGMN G LSFISQ    KFSYC+      D  G   LGD    + F ++  L + P 
Sbjct: 206 TGLLGMNRGSLSFISQMGFPKFSYCISGT--DDFPGFLLLGD----SNFTWLTPLNYTPL 265

Query: 181 SRLS---PNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDE 240
            R+S   P  D+ AYT+ + GI++    L I  +V  PD +GAGQTM+DSG+  T+L+  
Sbjct: 266 IRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGP 325

Query: 241 AYSKVREEMVRLVGPMM----KKGYEYAAVADMCF---DGAVAAAVGRRIGDMWFQFENG 300
            Y+ +R   +     ++       + +    D+C+      + + +  R+  +   FE G
Sbjct: 326 VYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFE-G 385

Query: 301 VEILVGKGEGLLTEV------EEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNK 345
            EI V  G+ LL  V       + V C   G SD +  E+ +IG+ HQQNMW+E+DL   
Sbjct: 386 AEIAV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRS 442

BLAST of Csor.00g309470 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 215.3 bits (547), Expect = 7.7e-56
Identity = 131/369 (35.50%), Postives = 198/369 (53.66%), Query Frame = 0

Query: 1   MDMVVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLP 60
           + MV+DTGS+LSW+ C       SV      F+P  SS++S +PC++ +CR R  D  +P
Sbjct: 78  ISMVLDTGSELSWLHCKKSPNLGSV------FNPVSSSTYSPVPCSSPICRTRTRDLPIP 137

Query: 61  TSCDPTRH-CHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGC--------ATASTQ 120
            SCDP  H CH +  YAD T  EGNL  E F    S+T    L GC        +    +
Sbjct: 138 ASCDPKTHLCHVAISYADATSIEGNLAHETFVI-GSVTRPGTLFGCMDSGLSSNSEEDAK 197

Query: 121 NRGMLGMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNPNSAKFKYVNMLTFP- 180
           + G++GMN G LSF++Q   SKFSYC+   +GSD +G   LGD    A + ++  + +  
Sbjct: 198 STGLMGMNRGSLSFVNQLGFSKFSYCI---SGSDSSGFLLLGD----ASYSWLGPIQYTP 257

Query: 181 ---KSRLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVD 240
              +S   P  D+ AYT+ ++GIR+G+  L++  +VF PD +GAGQTM+DSG+  T+L+ 
Sbjct: 258 LVLQSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMG 317

Query: 241 EAYSKVREEMVRLVGPMMK----KGYEYAAVADMCFDGAVAAAVGRRIGDMWFQFENGVE 300
             Y+ ++ E +     +++      + +    D+C+              M      G E
Sbjct: 318 PVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAE 377

Query: 301 ILVGKGEGLL-------TEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKR 345
           + V  G+ LL       +E +E V C   G SD L  E+ +IG+ HQQN+W+E+DL+  R
Sbjct: 378 MSV-SGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSR 431

BLAST of Csor.00g309470 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 143.7 bits (361), Expect = 2.9e-34
Identity = 112/359 (31.20%), Postives = 171/359 (47.63%), Query Frame = 0

Query: 4   VVDTGSQLSWIQCHGKVRRKSVKPMINWFDPYLSSSFSFLPCNTTLCRPRIPDFTLPTS- 63
           +VDTGS L W QC  K   +        FDP  SSS+S + C++ LC        LP S 
Sbjct: 123 IVDTGSDLIWTQC--KPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCN------ALPRSN 182

Query: 64  CDPTRH-CHYSYFYADGTLAEGNLVTEKFTFSNSLTTRSLLLGCATAS-----TQNRGML 123
           C+  +  C Y Y Y D +   G L TE FTF +  +   +  GC   +     +Q  G++
Sbjct: 183 CNEDKDACEYLYTYGDYSSTRGLLATETFTFEDENSISGIGFGCGVENEGDGFSQGSGLV 242

Query: 124 GMNTGRLSFISQAKISKFSYCVPDRTGSDLTGLFYLGDNP----NSAKFKYVNMLTFPKS 183
           G+  G LS ISQ K +KFSYC+     S+ +   ++G       N         +T   S
Sbjct: 243 GLGRGPLSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMS 302

Query: 184 RLSPNLDKSAYTLPMKGIRIGNNKLNISPAVFKPDPSGAGQTMIDSGSDLTYLVDEAYSK 243
            L      S Y L ++GI +G  +L++  + F+    G G  +IDSG+ +TYL + A+  
Sbjct: 303 LLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKV 362

Query: 244 VREEMV-RLVGPMMKKGYEYAAVADMCF---DGAVAAAVGRRIGDMWFQFENGVEILVGK 303
           ++EE   R+  P+   G   +   D+CF   D A   AV + I    F F+ G ++ +  
Sbjct: 363 LKEEFTSRMSLPVDDSG---STGLDLCFKLPDAAKNIAVPKMI----FHFK-GADLELPG 422

Query: 304 GEGLLTEVEEGVKCVGIGRSDRLVTESNIIGNVHQQNMWVEYDLSNKRVGFGVAKCSGL 348
              ++ +   GV C+ +G S+ +    +I GNV QQN  V +DL  + V F   +C  L
Sbjct: 423 ENYMVADSSTGVLCLAMGSSNGM----SIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZL31.4e-5736.31Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1[more]
Q766C34.8e-3430.84Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C25.3e-3331.14Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LS401.7e-3129.31Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LTW41.3e-2828.65Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE... [more]
Match NameE-valueIdentityDescription
KAG6607601.17.40e-260100.00Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6607573.11.06e-258100.00Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6607571.11.06e-258100.00Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022932084.11.51e-25899.71aspartic proteinase PCS1-like [Cucurbita moschata] >XP_022932086.1 aspartic prot... [more]
XP_022932085.11.51e-25899.71aspartic proteinase PCS1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1EWZ67.31e-25999.71aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438863 PE=3... [more]
A0A6J1F1P37.31e-25999.71aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438856 PE=3... [more]
A0A6J1F1817.31e-25999.71aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438406 PE=3... [more]
A0A6J1F0M71.04e-25899.43aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438404 PE=3... [more]
A0A6J1F1661.20e-25899.43aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111438397 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G37540.12.1e-13065.61Eukaryotic aspartyl protease family protein [more]
AT1G66180.11.9e-12365.32Eukaryotic aspartyl protease family protein [more]
AT5G02190.19.8e-5936.31Eukaryotic aspartyl protease family protein [more]
AT2G39710.17.7e-5635.50Eukaryotic aspartyl protease family protein [more]
AT2G03200.12.9e-3431.20Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 154..348
e-value: 9.2E-39
score: 134.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 1..153
e-value: 1.3E-27
score: 99.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 3..345
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 182..339
e-value: 4.9E-31
score: 107.7
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 2..154
e-value: 1.9E-29
score: 103.1
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 2..345
NoneNo IPR availablePANTHERPTHR47965:SF51EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 2..345
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 1..340
score: 26.513563
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 2..344
e-value: 2.54477E-66
score: 208.271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g309470.m01Csor.00g309470.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity