Csor.00g309050 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g309050
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	(R)-mandelonitrile lyase
Location	Csor_Chr15: 9408554 .. 9412186 (-)
RNA-Seq Expression	Csor.00g309050
Synteny	Csor.00g309050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTTGCATGGCGGAATTTCTTCTCTGCTGCTCTCGCCGGAATTCTCCTCTGCCATGTCTGTTCTTCCGCTCAAAAAGGTCCCAAATTTGAAATTCAAAACCTTTTAACTTCTAATTATTTTAATTACTTCATTCAGTTTGATCTTTGAGTTTAATTCTATGTATTATTACTTCAAATTTTAGCTAAATTATGAAAATGAAGAGCCATAAATTCTCTGTTTAAAAGTTCCTCTGTTTTTGCAGTTCCCAAATTCGATTTCATGCGAAATGCAGCAGAGGCTCCGGCGGTGTCACACTACGACTACATAATCGTAGGAGGTGGGACGGCGGGATGTCCGTTGGCGGCGACGCTGTCGGAAAATCACAAGGTGTTGATGTTAGAACGCGGTGGTTCTCCGTACGGAAACTCCAATATCACCAACTTATCGGCCTTCGGCGCTGCACTTGCCGACCTGTCGGAATCGTCGCCGTCGCAACGTTTCGTGTCGGAGGACGGCGTGATCAACTCTCGCGCACGTGTCCTCGGCGGCGGAAGCTGCCTGAATGCCGGATTTTACTCACGTGCGTCACTTGATTACGTCAGGTTCTCGTCGCACTACAAAAGTACACGCAATAATTTTAAAAAAAGTAATTGAAATGAAAATTGTGATTGGAATTGTTTGATAGGGAGGCGGGATGGGACGCGAAGTTGGTGAACGAGTCGTATGAGTGGGTGGAGCGGGTGGTGGCGTTCGAACCGCGGATGGGGAAGTGGCAGTCGGCGGTGAGGGGCGGTTTGGTTGATGTGGGTGTGGTGCCTGATAATGGGTTCACCTATGATCACTTATATGGTACTAAAGTGGGTGGCACCATTTTCGATCGTCACGGACATAGACACACGGCTGCTGATCTTTTGACCTATGCCAACCCTCCTAATCTAACCCTCTTCCTCTACGCCACTGCACATACCATCCTTTTCCAAACTAAAGGTAACTCCAATTACCCCGTTTTGCTCGAGTTGCTATAATTTTTATGTCTAATTATTTGCGGGTTTTGCTCGAATTGCCATATTTTTTTAGACTCTGCTTATTTGTTCATGGGTTTTGCTCGGGTTGCCCTATTTTTTAATGCCCAATTGTTCAGGGGTGTTCCTCGAGTTGCCATACTTTTTATGTTGCGGGTTTGGCTCATATTGCCATATTTTTTTAGACTATGCTTAATTGTTCATGGATTTTGCTCGAGTTGCCATATGTTTTGGACTCTGCCCAATTGTTCATGAGTTTTGCTGCCATATTTTTTAGTCTCTGCCCAATTGTTCACGGGTTTTACTCGGGTTGCAATATTTTTTTAGACTCTATCCAATTATTCGCGGGTTGCTATATTTTTTTAGACTCTACTCAATTGTTCACTAGTTTTGTTTGGATTGCCATCTTTTTTAGACTAATGCCCAACTGCTCATGGATTTAGCTCTGGTTGCCATGTTTTTTGGACTCTACCAAATTATTCACGGGTTTTTGTTGGAATTGCCATATTTATAAATTTTTTAAACTCAATTTCAACTTAATTATTTATTTAAAAGTTATTTTGTAATATAATTATTAATTTAAAATAAATTTAAATGCTGGTTTCCGTTTTTTTTTTTTAATAGGACGCAAAAGGCCAAGGGCCCACGGAGTGGTCTTCGAAGACTCGAAGGGAATCAAACACAGAGCGTACCTAAGGAATGGGCCTGAGAGCGAAGTAATCATATCAGCGGGCTGTCTTGGAAGCCCACAACTTCTGATGCTAAGTGGACTGGGCCCGGCCCAACATCTAAAGGCCCATAACATTACGGTGCTTTTGGATCAGCCCATGGTCGGGCAGAGGGTGTCCGATAACCCAATGAACGCCGTTTTCGTTCCATCCCCCGTTCCGGTGGAGGTGTCGTTGATTGAGGTCGTCGGAATTACCCATAACGGAACGTACATCGAAGCCGCCAGCGGCGAGAGCTTCGCCGGCGGCCCTTCTTCCAGAGACTTCGGCATGTTCTCTCCTAAGGTATGGATTTTACAATTATACATACATACATATATATATATATATATATATATAATTTTTTTTTTTTTTAATTTTGTAGACTTTATAAACTAAATTTTTTCATAAAATTTATGAATTTTGTGTTTTAATTGAATATTAAAGTTTAAAATTGATTAGTAATTTAAGGAAATAAAGTTGTGGGCAGATCGGTCAGCTATCGACAGTGCCACCGAAGCAAAGAACACCAGAAGCCATAGCCGAAGCCTGGGAGCTTATGAACAAACTGGACGAAGCCGCATTCAGAGGCGGCTTCATCCTTGAAAAGATAATGGGCCCACTCTCCTCGGGTCATTTGGAGCTCCGAACCCGAGACCCGAACGACAACCCGTCCGTCACATTCAACTACTTCAAAGATCCGACTGACCTACAACGCTGCGTCGCCGGCATAAACATAATCCGGCGAATCATCGAGTCAAAATCCTTCGCCAAGTTCCGATACCAAAACGTGTCGATGGCGACGCTTCTGAACATGACGGCGAGTGCTCCGATCAACCTGCTGCCGAAGCACGACAACTTGTCGAGGTCGACGGAGCAGTACTGCAGAGACACGGTGATGACGATCTGGCATTACCACGGCGGCTGCCAGACGGGGTCGGTGGTGGATTCCGATTACAGGGTTTTGGGAGTGGATTCGTTACGGGTTGTTGATGGCTCCACTTTTCATGATTCCCCTGGAACTAACCCTCAGGCTACTGTCATGATGCTCGGCAGGTATGTTTTTCTTTCATTCCTATTCCACCTAAATTACCAATTTCTCCCCATAATTTTTGGGTTCGGTTTTTATTTGGTTCTTATGATTTTAATCGAACCATTCACCTAAAAAAAATAACTAAAATTACTTCTATATTATATTTAAAAAGAAATAAAAAGTTAGTGATCAAACTATCAGTGTTCATATGACTCAATAATCGGAGTAATACAAGTTACACAACTCAAACTAAACAGTTTTTTTTGGTTTGAATTAAGGGTTGACATTTTTTTTAATCGGGTCAAGCTGAAAATAGTGTTACAACTCAAATCTACTTTTAAAAATATTAAAAAAATTTTATTTGAATTTTCAGAAATTATTTATTAATGTAATATATTCCGTGAATAAGTAAATATTTTTTAAATAATTAAAATAATTATATAAGACAACTGAATCAACTAAAAAGTAGAGAGTTGGATTGAGTCGTGTGATTTTTTCAACCTAACTCTGTATACCTAAATGAGATACTAATACGTTTTCTATTTTTATTAAAGAATATTAAATTTTAATAACAAAAATATATTTTTATAAATTAAGTTTTTTTTTTTAGATCTTAACAATTTATTTTTCGTTCTTTATTATTTATTTTAAAAATTTATACCCTTTGTTTCCTTTCCATTTTTTAATTTAAAAAAAAAGTATTAATTGATTTTTTAAATTGAAAGTCTTGAAAGTATACTTATAGGGAGAAAATCGTAATTATGGATCAAATTAATGATTTAAACCTTATTAATTGTATTTTTTTTTCTTTTATTTTTGGCAATTTTGCAGGTACATGGGAGTCAGAATATCGAGGGAAAGGTTTGAGAAAAGCACTGAAAAATAA

mRNA sequence

ATGGGTTTTGCATGGCGGAATTTCTTCTCTGCTGCTCTCGCCGGAATTCTCCTCTGCCATGTCTGTTCTTCCGCTCAAAAAGTTCCCAAATTCGATTTCATGCGAAATGCAGCAGAGGCTCCGGCGGTGTCACACTACGACTACATAATCGTAGGAGGTGGGACGGCGGGATGTCCGTTGGCGGCGACGCTGTCGGAAAATCACAAGGTGTTGATGTTAGAACGCGGTGGTTCTCCGTACGGAAACTCCAATATCACCAACTTATCGGCCTTCGGCGCTGCACTTGCCGACCTGTCGGAATCGTCGCCGTCGCAACGTTTCGTGTCGGAGGACGGCGTGATCAACTCTCGCGCACGTGTCCTCGGCGGCGGAAGCTGCCTGAATGCCGGATTTTACTCACGTGCGTCACTTGATTACGTCAGGGGTGTTCCTCGAGTTGCCATACTTTTTATGTTGCGGGCCCATAACATTACGGTGCTTTTGGATCAGCCCATGGTCGGGCAGAGGGTGTCCGATAACCCAATGAACGCCGTTTTCGTTCCATCCCCCGTTCCGGTGGAGGTGTCGTTGATTGAGGTCGTCGGAATTACCCATAACGGAACGTACATCGAAGCCGCCAGCGGCGAGAGCTTCGCCGGCGGCCCTTCTTCCAGAGACTTCGGCATGTTCTCTCCTAAGATCGGTCAGCTATCGACAGTGCCACCGAAGCAAAGAACACCAGAAGCCATAGCCGAAGCCTGGGAGCTTATGAACAAACTGGACGAAGCCGCATTCAGAGGCGGCTTCATCCTTGAAAAGATAATGGGCCCACTCTCCTCGGGTCATTTGGAGCTCCGAACCCGAGACCCGAACGACAACCCGTCCGTCACATTCAACTACTTCAAAGATCCGACTGACCTACAACGCTGCGTCGCCGGCATAAACATAATCCGGCGAATCATCGAGTCAAAATCCTTCGCCAAGTTCCGATACCAAAACGTGTCGATGGCGACGCTTCTGAACATGACGGCGAGTGCTCCGATCAACCTGCTGCCGAAGCACGACAACTTGTCGAGGTCGACGGAGCAGTACATGGGAGTCAGAATATCGAGGGAAAGGTTTGAGAAAAGCACTGAAAAATAA

Coding sequence (CDS)

ATGGGTTTTGCATGGCGGAATTTCTTCTCTGCTGCTCTCGCCGGAATTCTCCTCTGCCATGTCTGTTCTTCCGCTCAAAAAGTTCCCAAATTCGATTTCATGCGAAATGCAGCAGAGGCTCCGGCGGTGTCACACTACGACTACATAATCGTAGGAGGTGGGACGGCGGGATGTCCGTTGGCGGCGACGCTGTCGGAAAATCACAAGGTGTTGATGTTAGAACGCGGTGGTTCTCCGTACGGAAACTCCAATATCACCAACTTATCGGCCTTCGGCGCTGCACTTGCCGACCTGTCGGAATCGTCGCCGTCGCAACGTTTCGTGTCGGAGGACGGCGTGATCAACTCTCGCGCACGTGTCCTCGGCGGCGGAAGCTGCCTGAATGCCGGATTTTACTCACGTGCGTCACTTGATTACGTCAGGGGTGTTCCTCGAGTTGCCATACTTTTTATGTTGCGGGCCCATAACATTACGGTGCTTTTGGATCAGCCCATGGTCGGGCAGAGGGTGTCCGATAACCCAATGAACGCCGTTTTCGTTCCATCCCCCGTTCCGGTGGAGGTGTCGTTGATTGAGGTCGTCGGAATTACCCATAACGGAACGTACATCGAAGCCGCCAGCGGCGAGAGCTTCGCCGGCGGCCCTTCTTCCAGAGACTTCGGCATGTTCTCTCCTAAGATCGGTCAGCTATCGACAGTGCCACCGAAGCAAAGAACACCAGAAGCCATAGCCGAAGCCTGGGAGCTTATGAACAAACTGGACGAAGCCGCATTCAGAGGCGGCTTCATCCTTGAAAAGATAATGGGCCCACTCTCCTCGGGTCATTTGGAGCTCCGAACCCGAGACCCGAACGACAACCCGTCCGTCACATTCAACTACTTCAAAGATCCGACTGACCTACAACGCTGCGTCGCCGGCATAAACATAATCCGGCGAATCATCGAGTCAAAATCCTTCGCCAAGTTCCGATACCAAAACGTGTCGATGGCGACGCTTCTGAACATGACGGCGAGTGCTCCGATCAACCTGCTGCCGAAGCACGACAACTTGTCGAGGTCGACGGAGCAGTACATGGGAGTCAGAATATCGAGGGAAAGGTTTGAGAAAAGCACTGAAAAATAA

Protein sequence

MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPLAATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARVLGGGSCLNAGFYSRASLDYVRGVPRVAILFMLRAHNITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGPSSRDFGMFSPKIGQLSTVPPKQRTPEAIAEAWELMNKLDEAAFRGGFILEKIMGPLSSGHLELRTRDPNDNPSVTFNYFKDPTDLQRCVAGINIIRRIIESKSFAKFRYQNVSMATLLNMTASAPINLLPKHDNLSRSTEQYMGVRISRERFEKSTEK

Homology

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BLAST of Csor.00g309050 vs. ExPASy Swiss-Prot
Match: Q9S746 (Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.4e-60
Identity = 163/462 (35.28%), Postives = 215/462 (46.54%), Query Frame = 0


Query: 44  SHYDYIIVGGGTAGCPLAATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSP 103
           S YDYI++GGGTAGCPLAATLS+N  VL+LERGG P+ N+N++ L  F   LAD+S SS 
Sbjct: 61  SSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLADISASSA 120

Query: 104 SQRFVSEDGVINSRARVLGGGSCLNAGFYSRA---------------------------- 163
           SQ FVS DGV N+RARVLGGGSC+NAGFYSRA                            
Sbjct: 121 SQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAGWDPKLVKESYPWVEREIVH 180

Query: 164 --------------------------SLDYVRGV-------------------------- 223
                                     + D+V G                           
Sbjct: 181 QPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQ 240

Query: 224 ---------------------PRV-AILFM------------------------------ 283
                                PRV  ++F                               
Sbjct: 241 KLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQ 300

Query: 284 ------------LRAHNITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHN 343
                       L+   I V+L+   VG+ ++DNPMN + VPS  P+E SLI+ VGIT  
Sbjct: 301 MLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKM 360

Query: 344 GTYIEAASGESFAGGPSS--RDFGMFSPKIGQLSTVPPKQRTPEAIAEAWELMNKLD-EA 358
           G Y+EA++G  F   P S    +G+ S K    ST+P KQR PEA  +A+   NK     
Sbjct: 361 GVYVEASTG--FGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEA-TQAYITRNKYQLHE 420

[Close]

BLAST of Csor.00g309050 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.3e-37
Identity = 139/469 (29.64%), Postives = 180/469 (38.38%), Query Frame = 0


Query: 12  ALAGILLCHVCSSAQKVPKFD-------FMRNAAEAPAVSHYDYIIVGGGTAGCPLAATL 71
           AL  +LL  V   +   P+ +       F+ NA +  +  +YDYIIVGGGTAGCPLAATL
Sbjct: 13  ALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATL 72

Query: 72  SENHKVLMLERGGSPYGNSNITNLSAFGAALADLSE-SSPSQRFVSEDGVINSRARVLGG 131
           S++ +VL+LERGG PY   N+ +   F   L D++   SP+Q F+SE+GV N+R RVLGG
Sbjct: 73  SQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGG 132

Query: 132 GSCLNAGFYSRAS----------------------------------------------- 191
            S +NAGFYSRA                                                
Sbjct: 133 SSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEV 192

Query: 192 -----------------------------------LDYVRG----------VPRV----- 251
                                              L Y R           V RV     
Sbjct: 193 GVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASS 252

Query: 252 -------------------------------------------AILFM--------LRAH 311
                                                       +LF+        L   
Sbjct: 253 PSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTW 312

Query: 312 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 323
            I V LDQP VG  V DNP N + +  PVP+E SLI+VVG+T +G ++EAAS       P
Sbjct: 313 GIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASP 372

[Close]

BLAST of Csor.00g309050 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.4e-23
Identity = 116/451 (25.72%), Postives = 163/451 (36.14%), Query Frame = 0


Query: 23  SSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPLAATLSENHKVLMLERGGSPYGN 82
           +SA      +F+ +A +      YDYIIVGGGTAGCPLAATLS N+ VL+LERG  P   
Sbjct: 32  TSAHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGCPLAATLSANYSVLVLERGTLPTEY 91

Query: 83  SNITNLSAFGAALADLSE-SSPSQRFVSEDGVINSRARVLGGGSCLNAGFYSRA------ 142
            N+     F   L    +  +P +RFVS DG+ N R RVLGG S +NAG Y RA      
Sbjct: 92  PNLLTSDGFIYNLQQEDDGQTPVERFVSGDGIDNVRGRVLGGTSMINAGVYVRANTSFFN 151

Query: 143 --------------------------------------------------SLDYVR---- 202
                                                             SLD++     
Sbjct: 152 QTGIEWDMDLVNKTYDWVEDTIVFKPDFQFWQNLTGTAFLEVGILPDNGFSLDHLEGTRL 211

Query: 203 --------------------------------------------GVPRVAILF------- 262
                                                       GV  + +++       
Sbjct: 212 TGSTFDNNGTRHASDELLNKGDPNNLRVAVHAAVEKIIFSSDSSGVTAIGVIYTDSNGTT 271

Query: 263 ---------------------------------MLRAHNITVLLDQPMVGQRVSDNPMNA 322
                                             L + NI+V+   P VGQ + DNP N 
Sbjct: 272 HQAFVRGDGEVILSAGPIGSPQLLLLSGVGLESYLTSLNISVVASHPYVGQYIYDNPRNF 331

Query: 323 VFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGPSSRDFGMFSPKIGQLSTVPPKQ 328
           + +  P P+E S + V+GIT +  Y  + S   F+  P    FG F           P  
Sbjct: 332 INILPPNPIEASTVTVLGIT-SDFYQCSISSLPFSTAP----FGFF-----------PNP 391

[Close]

BLAST of Csor.00g309050 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 5.3e-23
Identity = 115/440 (26.14%), Postives = 164/440 (37.27%), Query Frame = 0


Query: 33  FMRNAAEAPAVSHYDYIIVGGGTAGCPLAATLSENHKVLMLERGGSPYGNSNITNLSAFG 92
           F  +A +      YDY+IVGGGT+GCPLAATLSE +KVL+LERG  P    N+     F 
Sbjct: 41  FAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFV 100

Query: 93  AALADLSE-SSPSQRFVSEDGVINSRARVLGGGSCLNAGFYSRA---------------- 152
             L    +  +P +RFVSEDG+ N R RVLGG S +NAG Y+RA                
Sbjct: 101 YNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDL 160

Query: 153 ----------------------------------------SLD----------------- 212
                                                   SLD                 
Sbjct: 161 VNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGT 220

Query: 213 ----------------------------------------------------YVR----- 272
                                                               +VR     
Sbjct: 221 RHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEV 280

Query: 273 -------GVPRVAIL------FMLRAHNITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEV 328
                  G P++ +L        L + NI V+L  P VGQ + DNP N + +  P P+E 
Sbjct: 281 IVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEP 340

[Close]

BLAST of Csor.00g309050 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.5e-22
Identity = 113/440 (25.68%), Postives = 162/440 (36.82%), Query Frame = 0


Query: 33  FMRNAAEAPAVSHYDYIIVGGGTAGCPLAATLSENHKVLMLERGGSPYGNSNITNLSAFG 92
           F  +A +      YDY+IVGGGT+GCPLAATLSE +KVL+LERG  P    N+     F 
Sbjct: 41  FAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFV 100

Query: 93  AALADLSE-SSPSQRFVSEDGVINSRARVLGGGSCLNAGFYSRA---------------- 152
             L    +  +P +RFVSEDG+ N R RVLGG S +NAG Y+RA                
Sbjct: 101 YNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDL 160

Query: 153 ----------------------------------------SLD----------------- 212
                                                   SLD                 
Sbjct: 161 VNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGT 220

Query: 213 ----------------------------------------------------YVR----- 272
                                                               +VR     
Sbjct: 221 RHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEV 280

Query: 273 -------GVPRVAIL------FMLRAHNITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEV 328
                  G P++ +L        L + NI V+L  P VGQ + DNP N + +  P P+E 
Sbjct: 281 IVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEP 340

[Close]

BLAST of Csor.00g309050 vs. NCBI nr
Match: KAG6579472.1 (Protein HOTHEAD, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 734 bits (1894), Expect = 8.12e-267
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL
Sbjct: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV
Sbjct: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120

Query: 121 LGGGSCLNAGFYSRASLDYVRGVPRVAILFMLRAHNITVLLDQPMVGQRVSDNPMNAVFV 180
           LGGGSCLNAGFYSRASLDYVRGVPRVAILFMLRAHNITVLLDQPMVGQRVSDNPMNAVFV
Sbjct: 121 LGGGSCLNAGFYSRASLDYVRGVPRVAILFMLRAHNITVLLDQPMVGQRVSDNPMNAVFV 180

Query: 181 PSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGPSSRDFGMFSPKIGQLSTVPPKQRTP 240
           PSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGPSSRDFGMFSPKIGQLSTVPPKQRTP
Sbjct: 181 PSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGPSSRDFGMFSPKIGQLSTVPPKQRTP 240

Query: 241 EAIAEAWELMNKLDEAAFRGGFILEKIMGPLSSGHLELRTRDPNDNPSVTFNYFKDPTDL 300
           EAIAEAWELMNKLDEAAFRGGFILEKIMGPLSSGHLELRTRDPNDNPSVTFNYFKDPTDL
Sbjct: 241 EAIAEAWELMNKLDEAAFRGGFILEKIMGPLSSGHLELRTRDPNDNPSVTFNYFKDPTDL 300

Query: 301 QRCVAGINIIRRIIESKSFAKFRYQNVSMATLLNMTASAPINLLPKHDNLSRSTEQYMGV 360
           QRCVAGINIIRRIIESKSFAKFRYQNVSMATLLNMTASAPINLLPKHDNLSRSTEQYMGV
Sbjct: 301 QRCVAGINIIRRIIESKSFAKFRYQNVSMATLLNMTASAPINLLPKHDNLSRSTEQYMGV 360

Query: 361 RISRERFEKSTEK 373
           RISRERFEKSTEK
Sbjct: 361 RISRERFEKSTEK 373

[Close]

BLAST of Csor.00g309050 vs. NCBI nr
Match: KAG7016941.1 (Protein HOTHEAD, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 628 bits (1619), Expect = 1.02e-221
Identity = 367/577 (63.60%), Postives = 369/577 (63.95%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL
Sbjct: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV
Sbjct: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120

Query: 121 LGGGSCLNAGFYSRASLDYVRGV---------------------PRVA------------ 180
           LGGGSCLNAGFYSRASLDYVR                       PR+             
Sbjct: 121 LGGGSCLNAGFYSRASLDYVREAGWDAKLVNESYEWVERVVAFEPRMGKWQSAVRGGLVD 180

Query: 181 -------------------------------------------------------ILFM- 240
                                                                  ILF  
Sbjct: 181 VGVVPDNGFTYDHLYGTKVGGTIFDRHGHRHTAADLLTYANPPNLTLFLYATAHTILFQT 240

Query: 241 --------------------------------------------------------LRAH 300
                                                                   L+AH
Sbjct: 241 KGRKRPRAHGVVFEDSKGIKHRAYLRNGPESEVIISAGCLGSPQLLMLSGLGPAQHLKAH 300

Query: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 360
           NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP
Sbjct: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 360

Query: 361 SSRDFGMFSPKIGQLSTVPPKQRTPEAIAEAWELMNKLDEAAFRGGFILEKIMGPLSSGH 373
           SSRDFGMFSPKIGQLSTVPPKQRTPEAIAEAWELMNKLDEAAFRGGFILEKIMGPLSSGH
Sbjct: 361 SSRDFGMFSPKIGQLSTVPPKQRTPEAIAEAWELMNKLDEAAFRGGFILEKIMGPLSSGH 420

[Close]

BLAST of Csor.00g309050 vs. NCBI nr
Match: XP_023549952.1 (protein HOTHEAD [Cucurbita pepo subsp. pepo])

HSP 1 Score: 621 bits (1602), Expect = 4.19e-219
Identity = 347/502 (69.12%), Postives = 351/502 (69.92%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           MGFAWRNFFSAAL GILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL
Sbjct: 1   MGFAWRNFFSAALTGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLSENHKVL+LERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVIN+RARV
Sbjct: 61  AATLSENHKVLLLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINARARV 120

Query: 121 LGGGSCLNAGFYSRASLDYVRGV---------------------PRVA------------ 180
           LGGGSCLNAGFYSRASLDYVR                       PR+             
Sbjct: 121 LGGGSCLNAGFYSRASLDYVREAGWDAKLVNESYEWVERVVAFEPRMGKWQSAVRGGLVD 180

Query: 181 -------------------------------------------------------ILFM- 240
                                                                  ILF  
Sbjct: 181 VGVVPDNGFTYDHLYGTKVGGTIFDRHGHRHTAADLLTYANPPNLTLFLYATAHTILFQT 240

Query: 241 --------------------------------------------------------LRAH 300
                                                                   L+AH
Sbjct: 241 KGRKRPRAHGVVFEDSKGIKHRAYLRNGPESEVIISAGCLGSPQLLMLSGLGPAQHLKAH 300

Query: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 357
           NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP
Sbjct: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 360

[Close]

BLAST of Csor.00g309050 vs. NCBI nr
Match: XP_022922265.1 (protein HOTHEAD [Cucurbita moschata])

HSP 1 Score: 620 bits (1600), Expect = 7.87e-219
Identity = 348/502 (69.32%), Postives = 351/502 (69.92%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL
Sbjct: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLSENH VLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV
Sbjct: 61  AATLSENHNVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120

Query: 121 LGGGSCLNAGFYSRASLDYVRGV---------------------PRVA------------ 180
           LGGGSCLNAGFYSRASLDYVR                       PR+             
Sbjct: 121 LGGGSCLNAGFYSRASLDYVREAGWDAKLVNESYEWVERVVAFEPRMGKWQSAVRGGLVD 180

Query: 181 -------------------------------------------------------ILFM- 240
                                                                  ILF  
Sbjct: 181 VGVVPDNGFTYDHLYGTKVGGTIFDRHGHRHTAADLLTYANPPNLTLFLYATAHTILFQT 240

Query: 241 --------------------------------------------------------LRAH 300
                                                                   L+AH
Sbjct: 241 KGRKRPRAHGVVFEDSKGIKHRAYLRNGPESEVIISAGCLGSPQLLMLSGLGPAQHLKAH 300

Query: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 357
           NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP
Sbjct: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 360

[Close]

BLAST of Csor.00g309050 vs. NCBI nr
Match: XP_022969860.1 (protein HOTHEAD [Cucurbita maxima])

HSP 1 Score: 612 bits (1577), Expect = 2.63e-215
Identity = 343/502 (68.33%), Postives = 349/502 (69.52%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           MGFAWRNFFSAAL GILLCH CSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL
Sbjct: 1   MGFAWRNFFSAALTGILLCHGCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLSEN+KVLMLERGGSP+GNSNITNLSAFGAALADLSESSPSQRFVSEDGVIN+RARV
Sbjct: 61  AATLSENYKVLMLERGGSPFGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINARARV 120

Query: 121 LGGGSCLNAGFYSRASLDYVRGV---------------------PRVA------------ 180
           LGGGSCLNAGFYSRASLDYVR                       PR+             
Sbjct: 121 LGGGSCLNAGFYSRASLDYVREAGWDAKLVNESYEWVERVVAFEPRMGKWQSAVRGGLVD 180

Query: 181 -------------------------------------------------------ILFM- 240
                                                                  ILF  
Sbjct: 181 VGVVPDNGFTYDHLYGTKVGGTIFDRHGHRHTAADLLTYANPPNLTLFLYATAHTILFQT 240

Query: 241 --------------------------------------------------------LRAH 300
                                                                   L+AH
Sbjct: 241 KGRKRPRAHGVVFEDSKGIKHRAYLRNGPESEVIISAGCLGSPQLLMLSGLGPAQHLKAH 300

Query: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 357
           NITVLLDQPMVGQRVSDNPMNAVFVPSPV VEVSLIEVVGITHNGTYIEAASGESFAGGP
Sbjct: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVSVEVSLIEVVGITHNGTYIEAASGESFAGGP 360

[Close]

BLAST of Csor.00g309050 vs. ExPASy TrEMBL
Match: A0A6J1E887 (protein HOTHEAD OS=Cucurbita moschata OX=3662 GN=LOC111430298 PE=3 SV=1)

HSP 1 Score: 620 bits (1600), Expect = 3.81e-219
Identity = 348/502 (69.32%), Postives = 351/502 (69.92%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL
Sbjct: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLSENH VLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV
Sbjct: 61  AATLSENHNVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120

Query: 121 LGGGSCLNAGFYSRASLDYVRGV---------------------PRVA------------ 180
           LGGGSCLNAGFYSRASLDYVR                       PR+             
Sbjct: 121 LGGGSCLNAGFYSRASLDYVREAGWDAKLVNESYEWVERVVAFEPRMGKWQSAVRGGLVD 180

Query: 181 -------------------------------------------------------ILFM- 240
                                                                  ILF  
Sbjct: 181 VGVVPDNGFTYDHLYGTKVGGTIFDRHGHRHTAADLLTYANPPNLTLFLYATAHTILFQT 240

Query: 241 --------------------------------------------------------LRAH 300
                                                                   L+AH
Sbjct: 241 KGRKRPRAHGVVFEDSKGIKHRAYLRNGPESEVIISAGCLGSPQLLMLSGLGPAQHLKAH 300

Query: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 357
           NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP
Sbjct: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 360

[Close]

BLAST of Csor.00g309050 vs. ExPASy TrEMBL
Match: A0A6J1I159 (protein HOTHEAD OS=Cucurbita maxima OX=3661 GN=LOC111468930 PE=3 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 1.27e-215
Identity = 343/502 (68.33%), Postives = 349/502 (69.52%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           MGFAWRNFFSAAL GILLCH CSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL
Sbjct: 1   MGFAWRNFFSAALTGILLCHGCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLSEN+KVLMLERGGSP+GNSNITNLSAFGAALADLSESSPSQRFVSEDGVIN+RARV
Sbjct: 61  AATLSENYKVLMLERGGSPFGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINARARV 120

Query: 121 LGGGSCLNAGFYSRASLDYVRGV---------------------PRVA------------ 180
           LGGGSCLNAGFYSRASLDYVR                       PR+             
Sbjct: 121 LGGGSCLNAGFYSRASLDYVREAGWDAKLVNESYEWVERVVAFEPRMGKWQSAVRGGLVD 180

Query: 181 -------------------------------------------------------ILFM- 240
                                                                  ILF  
Sbjct: 181 VGVVPDNGFTYDHLYGTKVGGTIFDRHGHRHTAADLLTYANPPNLTLFLYATAHTILFQT 240

Query: 241 --------------------------------------------------------LRAH 300
                                                                   L+AH
Sbjct: 241 KGRKRPRAHGVVFEDSKGIKHRAYLRNGPESEVIISAGCLGSPQLLMLSGLGPAQHLKAH 300

Query: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGP 357
           NITVLLDQPMVGQRVSDNPMNAVFVPSPV VEVSLIEVVGITHNGTYIEAASGESFAGGP
Sbjct: 301 NITVLLDQPMVGQRVSDNPMNAVFVPSPVSVEVSLIEVVGITHNGTYIEAASGESFAGGP 360

[Close]

BLAST of Csor.00g309050 vs. ExPASy TrEMBL
Match: A0A1S3AT31 (protein HOTHEAD OS=Cucumis melo OX=3656 GN=LOC103482699 PE=3 SV=1)

HSP 1 Score: 528 bits (1359), Expect = 1.51e-182
Identity = 296/501 (59.08%), Postives = 326/501 (65.07%), Query Frame = 0


Query: 2   GFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPLA 61
           GF W  FF++AL   LL H  SS+Q+VPKF F+RNA +AP VS+YDYIIVGGGTAGCPLA
Sbjct: 3   GFVWGGFFTSALTAFLLFHGFSSSQQVPKFSFLRNATDAPMVSYYDYIIVGGGTAGCPLA 62

Query: 62  ATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARVL 121
           ATLSEN+KVLMLERGGSPYGN NITNLSAFGAAL+DLS SSPSQRFVSEDGVINSRARVL
Sbjct: 63  ATLSENYKVLMLERGGSPYGNPNITNLSAFGAALSDLSASSPSQRFVSEDGVINSRARVL 122

Query: 122 GGGSCLNAGFYSRASLDYVR---------------------------------------- 181
           GGGSCLNAGFY+RAS DYVR                                        
Sbjct: 123 GGGSCLNAGFYTRASSDYVRRAGWERRLVKESYEWVERVVAFEPPMGEWQSAVRDGLIEA 182

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 183 GVKPNNGFTYDHLYGTKVGGTIFDHHGHRHTAADLLSYANPSNLDVLLYASAHSIIFRPL 242

Query: 242 ---------------------------------------GVPRVAILF------MLRAHN 301
                                                  G P++ +L        L+AHN
Sbjct: 243 GEERPKAHGVVFEDSKGIKHRAYLKYGPKSEIIISAGCLGSPQLLMLSGLGPAQHLKAHN 302

Query: 302 ITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGPS 357
           ITV+LD PMVGQRVSDNPMNAVFVPSPVPVE+SLIEVVGIT NGTYIEAASGE+F GGPS
Sbjct: 303 ITVVLDHPMVGQRVSDNPMNAVFVPSPVPVELSLIEVVGITQNGTYIEAASGENFGGGPS 362

[Close]

BLAST of Csor.00g309050 vs. ExPASy TrEMBL
Match: A0A0A0KP09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G156120 PE=3 SV=1)

HSP 1 Score: 521 bits (1341), Expect = 8.07e-180
Identity = 294/501 (58.68%), Postives = 324/501 (64.67%), Query Frame = 0


Query: 2   GFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPLA 61
           G  W  FF++AL   LL H  SS+ +VPKF F+RNA +AP VS+YDYIIVGGGTAGCPLA
Sbjct: 3   GSVWGEFFTSALTAFLLFHGFSSSHQVPKFSFLRNATDAPTVSYYDYIIVGGGTAGCPLA 62

Query: 62  ATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARVL 121
           ATLS+ +KVL+LERGGSPYGN NITNLSAFGAAL+DLS SSPSQRFVSEDGVINSRARVL
Sbjct: 63  ATLSKKYKVLVLERGGSPYGNPNITNLSAFGAALSDLSASSPSQRFVSEDGVINSRARVL 122

Query: 122 GGGSCLNAGFYSRASLDYVR---------------------------------------- 181
           GGGSCLNAGFY+RAS DYVR                                        
Sbjct: 123 GGGSCLNAGFYTRASPDYVRRAGWEGKLVNESYEWVERVVAFEPPMGEWQSAVRDGLIEA 182

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 183 GVKPNNGFTYDHLYGTKVGGTIFDHHGHRHTAADLLSYANPSNLNVLLYATARSIIFPSL 242

Query: 242 ---------------------------------------GVPRVAILF------MLRAHN 301
                                                  G P++ +L        L+AHN
Sbjct: 243 GKRRPKAHGVVFEDSKGIKHRAYLKYGSKSEIIISAGCLGSPQLLMLSGLGPAQHLKAHN 302

Query: 302 ITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGPS 357
           ITV+LD PMVGQ VSDNPMNAVFVPSPVPVEVSLIEVVGIT NGTYIEAASGE+FAGGPS
Sbjct: 303 ITVVLDHPMVGQSVSDNPMNAVFVPSPVPVEVSLIEVVGITQNGTYIEAASGENFAGGPS 362

[Close]

BLAST of Csor.00g309050 vs. ExPASy TrEMBL
Match: A0A314Z6F2 ((R)-mandelonitrile lyase OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_19580 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 2.64e-170
Identity = 263/455 (57.80%), Postives = 307/455 (67.47%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           MGF W   F AAL GIL  H    A++ P F FM NA  AP +S+YDYIIVGGGTAGCPL
Sbjct: 1   MGFGWWKLFGAALVGILFFHGFCGAERAPDFSFMHNATTAPDISYYDYIIVGGGTAGCPL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLS+N+ VL+LERGG+PYGN NITNLSAFG AL+DLS +SPSQRF+SEDGVIN+RARV
Sbjct: 61  AATLSQNYSVLLLERGGTPYGNPNITNLSAFGRALSDLSPTSPSQRFISEDGVINARARV 120

Query: 121 LGGGSCLNAGFYSRASLDYVR--------------------------------------- 180
           LGGGSCLNAGFYSRA+ DY R                                       
Sbjct: 121 LGGGSCLNAGFYSRAATDYAREAGWDTRLVNESYQWVERLVAFRPPMQAWQSAVRDGLTV 180

Query: 181 -----------------------------------------------------GVPRVAI 240
                                                                G P++ +
Sbjct: 181 LLHATVHKILFRTKGKSRPLAHGVVFRDASGNKHRAYLKSGPKNEIIVSSGALGSPQLLM 240

Query: 241 LF------MLRAHNITVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTY 300
           L        LRAHNITV++DQP+VGQ +SDNPMNA+FVPSP+PVEVSLI+VVGITH G+Y
Sbjct: 241 LSGVGPAEHLRAHNITVVVDQPLVGQGMSDNPMNAIFVPSPIPVEVSLIQVVGITHFGSY 300

Query: 301 IEAASGESFAGGPSSRDFGMFSPKIGQLSTVPPKQRTPEAIAEAWELMNKLDEAAFRGGF 357
           IEAASGE+FAG  S+RDFGMFSPKIGQLSTVPPKQRTPEA+A+A E MN L+  AFRGGF
Sbjct: 301 IEAASGENFAGSASTRDFGMFSPKIGQLSTVPPKQRTPEALAKAVEYMNSLEAPAFRGGF 360

[Close]

BLAST of Csor.00g309050 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 344.4 bits (882), Expect = 1.2e-94
Identity = 216/497 (43.46%), Postives = 262/497 (52.72%), Query Frame = 0


Query: 3   FAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPLAA 62
           ++WR F   AL+  L   +CSS  K P + FMR+A  +P  S+YDYII+GGGTAGCPLAA
Sbjct: 6   WSWRLF--VALSLFLHSPICSS-DKAPNYSFMRDATGSPTTSYYDYIIIGGGTAGCPLAA 65

Query: 63  TLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARVLG 122
           TLS+N  VL+LERG SPY N NIT LSAFGAAL+DLSESSPSQRFVSEDGVIN+RARVLG
Sbjct: 66  TLSQNASVLLLERGDSPYNNPNITRLSAFGAALSDLSESSPSQRFVSEDGVINARARVLG 125

Query: 123 GGSCLNAGFYSRASLDYVR----------------------------------------- 182
           GGS LNAGFY+RA   YVR                                         
Sbjct: 126 GGSALNAGFYTRAGTKYVRNMGWDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAG 185

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 186 IVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRG 245

Query: 243 --------------------------------------GVPRVAIL------FMLRAHNI 302
                                                 G P++ +L        L+A NI
Sbjct: 246 TTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNI 305

Query: 303 TVLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGG--- 346
           TV++DQP VGQ + DNPMNAVFVPSPVPVEVSLIEVVGIT  GTY+EAA GE+F GG   
Sbjct: 306 TVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGG 365

[Close]

BLAST of Csor.00g309050 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.1e-68
Identity = 171/480 (35.62%), Postives = 227/480 (47.29%), Query Frame = 0


Query: 20  HVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPLAATLSENHKVLMLERGGSP 79
           H   S  K   + FM++A  AP  + +DYII+GGGT+GC LAATLS+N  VL+LERGG+P
Sbjct: 20  HASCSPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAP 79

Query: 80  YGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARVLGGGSCLNAGFYSRASLDY 139
           Y N   T++  F   L++ S  S SQ F+SEDGV N+RARVLGGGS LNAGFY+RA  +Y
Sbjct: 80  YDNPTATDIENFATTLSNTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEY 139

Query: 140 V----------------------------------------------------------- 199
           V                                                           
Sbjct: 140 VKETEWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKI 199

Query: 200 -----------------------------------------RGVPR---VAILFM----- 259
                                                    +G PR     ++F      
Sbjct: 200 GGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGV 259

Query: 260 -------------------------------------LRAHNI-TVLLDQPMVGQRVSDN 319
                                                L AH I  ++LD PMVGQ + DN
Sbjct: 260 LHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDN 319

Query: 320 PMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFAGGPSSRDFGMFSPKIGQL--- 348
           PMNA+F+PSP PVEVSLI+VVGIT   +YIE ASG  F+   + R F      + ++   
Sbjct: 320 PMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTS 379

[Close]

BLAST of Csor.00g309050 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 254.2 bits (648), Expect = 1.6e-67
Identity = 166/505 (32.87%), Postives = 236/505 (46.73%), Query Frame = 0


Query: 1   MGFAWRNFFSAALAGILLCHVCSSAQKVPKFDFMRNAAEAPAVSHYDYIIVGGGTAGCPL 60
           M F    FF   L  + + H    + +   + FM++A  AP +SH+DYII+GGGTAGC L
Sbjct: 1   MNFHIFRFFQFILVAVFIFHDSCYSDEAGNYRFMKDATLAPKLSHFDYIIIGGGTAGCAL 60

Query: 61  AATLSENHKVLMLERGGSPYGNSNITNLSAFGAALADLSESSPSQRFVSEDGVINSRARV 120
           AATLS+N  VL+LERGGSPY +   T++  F   L +++ +S SQ F+SEDGV NSRARV
Sbjct: 61  AATLSQNATVLVLERGGSPYDDPAATDIGNFANTLLNITPNSWSQLFISEDGVFNSRARV 120

Query: 121 LGGGSCLNAGFYSRASLDYV---------------------------------------- 180
           LGGG+ +NAGFYSRA  D+V                                        
Sbjct: 121 LGGGTVINAGFYSRAEEDFVAEAGWERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLE 180

Query: 181 RGV--------------------------------------PRVAILFM----------- 240
            GV                                      P + ++++           
Sbjct: 181 AGVTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKILFTI 240

Query: 241 ----------------------------------------------------------LR 300
                                                                     L 
Sbjct: 241 KGNQRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGPAAHLA 300

Query: 301 AHNIT-VLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIEVVGITHNGTYIEAASGESFA 358
           A+ +  V++DQPMVGQ + DNPMN VF+PSP PVEVSL++ VGIT  G+YIE  S  S +
Sbjct: 301 AYRVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSALSLS 360

[Close]

BLAST of Csor.00g309050 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.3e-66
Identity = 167/466 (35.84%), Postives = 222/466 (47.64%), Query Frame = 0


Query: 34  MRNAAEAPAVSHYDYIIVGGGTAGCPLAATLSENHKVLMLERGGSPYGNSNITNLSAFGA 93
           M++A  AP  + +DYII+GGGT+GC LAATLS+N  VL+LERGG+PY N   T++  F  
Sbjct: 1   MKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFAT 60

Query: 94  ALADLSESSPSQRFVSEDGVINSRARVLGGGSCLNAGFYSRASLDYV------------- 153
            L++ S  S SQ F+SEDGV N+RARVLGGGS LNAGFY+RA  +YV             
Sbjct: 61  TLSNTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKETEWKTDEVEAA 120

Query: 154 ------------------------------------------------------------ 213
                                                                       
Sbjct: 121 YEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTA 180

Query: 214 ---------------------------RGVPR---VAILFM------------------- 273
                                      +G PR     ++F                    
Sbjct: 181 ADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEV 240

Query: 274 -----------------------LRAHNI-TVLLDQPMVGQRVSDNPMNAVFVPSPVPVE 333
                                  L AH I  ++LD PMVGQ + DNPMNA+F+PSP PVE
Sbjct: 241 ILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVE 300

Query: 334 VSLIEVVGITHNGTYIEAASGESFAGGPSSRDFGMFSPKIGQL------STVPPKQRTPE 348
           VSLI+VVGIT   +YIE ASG  F+   + R F      + ++      ST  P   T +
Sbjct: 301 VSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLST-Q 360

[Close]

BLAST of Csor.00g309050 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 236.1 bits (601), Expect = 4.5e-62
Identity = 156/465 (33.55%), Postives = 216/465 (46.45%), Query Frame = 0


Query: 33  FMRNAAEAPAVSHYDYIIVGGGTAGCPLAATLSENHKVLMLERGGSPYGNSNITNLSAFG 92
           FM++A  AP  + +DYII+GGGTAGC LAATLS+N  VL+LERGGSPY N   T++    
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 93  AALADLSESSPSQRFVSEDGVINSRARVLGGGSCLNAGFYSRASLDYVR----------- 152
             L + + +S SQ F+SEDGV N+R RVLGGGS +N GFYSRA  DYV            
Sbjct: 120 NTLLNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEAEWEMEEVEA 179

Query: 153 ------------------------------------------------------------ 212
                                                                       
Sbjct: 180 AYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGHRHT 239

Query: 213 ------------------------------------------------------------ 272
                                                                       
Sbjct: 240 AANLLEYANPNRIVVYLHASVHKVLFTTEAYEVLFEDANGVFHKANLANKATNEVILSAG 299

Query: 273 --GVPRVAIL------FMLRAHNIT-VLLDQPMVGQRVSDNPMNAVFVPSPVPVEVSLIE 332
             G P++ +L        L AH +  ++LDQPMVGQ ++DNPMN V +PSP PVE+SLI+
Sbjct: 300 ALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVELSLIQ 359

Query: 333 VVGITHNGTYIEAASGESFAGGPSSRDFGMFSPKIGQLSTVPPKQRTPEAIAEAWELMNK 358
            VGIT   +YIE  SG S +   + R F      + + S    ++   ++IA   +  + 
Sbjct: 360 AVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLLKSFDV 419

The following BLAST results are available for this feature:

BLAST of Csor.00g309050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S746	5.4e-60	35.28	Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1	[more]
Q9SSM2	1.3e-37	29.64	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
O50048	2.4e-23	25.72	(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1	[more]
P52706	5.3e-23	26.14	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	1.5e-22	25.68	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]

BLAST of Csor.00g309050 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6579472.1	8.12e-267	100.00	Protein HOTHEAD, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7016941.1	1.02e-221	63.60	Protein HOTHEAD, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023549952.1	4.19e-219	69.12	protein HOTHEAD [Cucurbita pepo subsp. pepo]	[more]
XP_022922265.1	7.87e-219	69.32	protein HOTHEAD [Cucurbita moschata]	[more]
XP_022969860.1	2.63e-215	68.33	protein HOTHEAD [Cucurbita maxima]	[more]

BLAST of Csor.00g309050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E887	3.81e-219	69.32	protein HOTHEAD OS=Cucurbita moschata OX=3662 GN=LOC111430298 PE=3 SV=1	[more]
A0A6J1I159	1.27e-215	68.33	protein HOTHEAD OS=Cucurbita maxima OX=3661 GN=LOC111468930 PE=3 SV=1	[more]
A0A1S3AT31	1.51e-182	59.08	protein HOTHEAD OS=Cucumis melo OX=3656 GN=LOC103482699 PE=3 SV=1	[more]
A0A0A0KP09	8.07e-180	58.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G156120 PE=3 SV=1	[more]
A0A314Z6F2	2.64e-170	57.80	(R)-mandelonitrile lyase OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_19...	[more]

BLAST of Csor.00g309050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12570.1	1.2e-94	43.46	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	1.1e-68	35.63	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT3G56060.1	1.6e-67	32.87	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.2	2.3e-66	35.84	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	4.5e-62	33.55	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 29..144 e-value: 2.6E-33 score: 117.4
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 39..193
None	No IPR available	GENE3D	3.30.410.40		coord: 158..359 e-value: 1.4E-44 score: 153.8
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 152..358 coord: 21..143
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 171..334
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 46..136 e-value: 2.4E-9 score: 37.0
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 270..326 e-value: 2.8E-8 score: 34.3

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g309050.m01	Csor.00g309050.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors