Csor.00g308690 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g308690
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionCytochrome P450 76C4
LocationCsor_Chr15: 9227555 .. 9229744 (+)
RNA-Seq ExpressionCsor.00g308690
SyntenyCsor.00g308690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACCCACCTCCAATTCTCTTCAAACCCGCATAGATTCCCTCAATTTCTTGCACCCATGGCGGTGGATTGACGCCGCCGATAACCAATCCCGTTTCCTCTTCACTGTCTTCTTCGCCGGTCTACTAATTTTCCTCTATACTAAAGTTACGCGTCGGCGCGTGCCTCTACCACCAGGTCCTCTGGGAGTTCCCCTGCTTGGAAACCTGCCGTTTCTGGACCCGGAACTTCACACCTATTTCAGAGACTTGGGGCAGAAATACGGGCCAATTGTAAAGCTCCAATTGGGTAATAAGGTGGGTATAATCGTCAATTCACCCTCCGTGGCACGCGAGATTTTGAAAGACAACGATGTCACGTTCGCCAATCGTGACGTTCCTCAGGCTGGGAGAGCCGCCACGTACGGTGGTTCCGACATAGCTTGGACCCCGTATGGAGACGAGTGGCGAATGTTGAGAAAAGTTTGTGTGGTCAAGATGCTGAGCAACGCCACTTTGGAGTCCGTATACGATCTCCGCCGTAGAGAGGTCAGAAACACCGTCGCTATCCTGTACGGGCGAGCCGGGTCGGCGATGAATGTGGGAGAGCAGAGTTTTCTGACGATTTTCAACGTGGTTACGAGCATGTTGTGGGGTGGGACAGTGAAGGGGGACCAGAGGGATAGCCTTGCAGCTGAGTTCAGAGAGACAGTTTCTGACTTGACTGAGCTACTTGGAAAGCCTAATGTTTCGGACTTTTTTCCGAGCTTGGCTCGTTTCGATCTCCAAGGCATTGAAAAGAAGATGCATAAGCTTGCTCACAAATTTGATACCATTTTTGAGAACATGATCAATCAACGATTGAAAATTGCCGGTGGGGCGGACGGCGAGAGTGCCAAGAATAAGGATTTCTTGCAGTTCTTACTTGAGGTGAAAGATTCCGGCGACTCCAAAACTCCTCTCACCATCACCCACCTCAAAGCCTTGCTCATGGTAACACTCATTTTAATCCCTCTTTTTATGTTATATTACAAGTTTATTGTGCTTTTAACTTATATAATTTTTAGTAGATTCCTTAAACCAGATGTATTCAATGAGTCTATAGATCCATCTTTGAAATTTCATCTTAATGGGATAGATCATTACAATAGTTGGTATTCAACCAGGAATTTCTAGTACGCGAGAGTGATCAGCTCGCGTTGGCTACATCCCTGCCCTTTGTACATACTGCCCGTCACTCCTACTGATTAAATGGTCCGATGAAGTGTTCGAATTACGGCGACGTAGGCAGTTCGCTGTCTGCGACGTCGCGAGAAGTCCACTTAAACTTATCATTTAGAGGAAGAAGTCGTAACAAGAATTGTTAGTAGATCTAGTAGAATAGAGTAACTAGTAGAGTTAATAGTAAGTTATGACTTATGCACCAACTACCACTAGTAGAATAGAGTGACAAATTGTGGACCAAATTTTATGTTTGTGGGCCATGTGCTTTAATTGTGAAGATTGCCGGTGATTCTTAATTCCTTAACTTATAGTTAATGTTTGTGGACAAAAATTTGAGAAAGGGAAGTGGGGTTGTGTGGGCAGGATATGGTGACTGGGGGGACAGACACATCGTCAAACACCATAGAGTTTGCGCTGGCAGAGATGATAAACAGCCCTGCAACTCTGAAGAAAGCACAGGAAGAAGTAGCTGCCGTGGTGGGAGAAGACAACATCGTGGAGGAGTCCCACATTCATAGTTTACCATATTTAAAAGCTGTAATGAAAGAAACTTTGCGTTTGCACCCAATTCTACCCCTGCTAGTGCCACACTCCCCAAGTGAGACCACCATCATCTCCAAATACACAATCCCAAAGGGCTCTCGTGTGTTCATCAATGTGTGGGCCATTCAGCGAGACCCCAACCAATGGGAAAATCCATTGGTGTTCGATCCGGAGAGGTTTCTGAACAACCAGAAGTGGGATTTCGGTGGGAGTGATTTTCGTTACTTCCCGTTCGGATCCGGCAGAAGAAACTGTGCAGGGATTGCCATGGCGGAAAGAACGTTCATGTACTTGCTTGCTACGCTCTTGCACTCCTTTGATTGGAAACTGGAAGAGGGTAAGAAACTGGAAATTGAGGAGAAATTTGGGATTGTTTTGAAGATGAAGACGCCTCTTGTTCTCGTCCCGACGCCCAGACTATCCGACCCTACTCTGTATCAGTAA

mRNA sequence

ATGGAACCCACCTCCAATTCTCTTCAAACCCGCATAGATTCCCTCAATTTCTTGCACCCATGGCGGTGGATTGACGCCGCCGATAACCAATCCCGTTTCCTCTTCACTGTCTTCTTCGCCGGTCTACTAATTTTCCTCTATACTAAAGTTACGCGTCGGCGCGTGCCTCTACCACCAGGTCCTCTGGGAGTTCCCCTGCTTGGAAACCTGCCGTTTCTGGACCCGGAACTTCACACCTATTTCAGAGACTTGGGGCAGAAATACGGGCCAATTGTAAAGCTCCAATTGGGTAATAAGGTGGGTATAATCGTCAATTCACCCTCCGTGGCACGCGAGATTTTGAAAGACAACGATGTCACGTTCGCCAATCGTGACGTTCCTCAGGCTGGGAGAGCCGCCACGTACGGTGGTTCCGACATAGCTTGGACCCCGTATGGAGACGAGTGGCGAATGTTGAGAAAAGTTTGTGTGGTCAAGATGCTGAGCAACGCCACTTTGGAGTCCGTATACGATCTCCGCCGTAGAGAGGTCAGAAACACCGTCGCTATCCTGTACGGGCGAGCCGGGTCGGCGATGAATGTGGGAGAGCAGAGTTTTCTGACGATTTTCAACGTGGTTACGAGCATGTTGTGGGGTGGGACAGTGAAGGGGGACCAGAGGGATAGCCTTGCAGCTGAGTTCAGAGAGACAGTTTCTGACTTGACTGAGCTACTTGGAAAGCCTAATGTTTCGGACTTTTTTCCGAGCTTGGCTCGTTTCGATCTCCAAGGCATTGAAAAGAAGATGCATAAGCTTGCTCACAAATTTGATACCATTTTTGAGAACATGATCAATCAACGATTGAAAATTGCCGGTGGGGCGGACGGCGAGAGTGCCAAGAATAAGGATTTCTTGCAGTTCTTACTTGAGGTGAAAGATTCCGGCGACTCCAAAACTCCTCTCACCATCACCCACCTCAAAGCCTTGCTCATGATGAAGACGCCTCTTGTTCTCGTCCCGACGCCCAGACTATCCGACCCTACTCTGTATCAGTAA

Coding sequence (CDS)

ATGGAACCCACCTCCAATTCTCTTCAAACCCGCATAGATTCCCTCAATTTCTTGCACCCATGGCGGTGGATTGACGCCGCCGATAACCAATCCCGTTTCCTCTTCACTGTCTTCTTCGCCGGTCTACTAATTTTCCTCTATACTAAAGTTACGCGTCGGCGCGTGCCTCTACCACCAGGTCCTCTGGGAGTTCCCCTGCTTGGAAACCTGCCGTTTCTGGACCCGGAACTTCACACCTATTTCAGAGACTTGGGGCAGAAATACGGGCCAATTGTAAAGCTCCAATTGGGTAATAAGGTGGGTATAATCGTCAATTCACCCTCCGTGGCACGCGAGATTTTGAAAGACAACGATGTCACGTTCGCCAATCGTGACGTTCCTCAGGCTGGGAGAGCCGCCACGTACGGTGGTTCCGACATAGCTTGGACCCCGTATGGAGACGAGTGGCGAATGTTGAGAAAAGTTTGTGTGGTCAAGATGCTGAGCAACGCCACTTTGGAGTCCGTATACGATCTCCGCCGTAGAGAGGTCAGAAACACCGTCGCTATCCTGTACGGGCGAGCCGGGTCGGCGATGAATGTGGGAGAGCAGAGTTTTCTGACGATTTTCAACGTGGTTACGAGCATGTTGTGGGGTGGGACAGTGAAGGGGGACCAGAGGGATAGCCTTGCAGCTGAGTTCAGAGAGACAGTTTCTGACTTGACTGAGCTACTTGGAAAGCCTAATGTTTCGGACTTTTTTCCGAGCTTGGCTCGTTTCGATCTCCAAGGCATTGAAAAGAAGATGCATAAGCTTGCTCACAAATTTGATACCATTTTTGAGAACATGATCAATCAACGATTGAAAATTGCCGGTGGGGCGGACGGCGAGAGTGCCAAGAATAAGGATTTCTTGCAGTTCTTACTTGAGGTGAAAGATTCCGGCGACTCCAAAACTCCTCTCACCATCACCCACCTCAAAGCCTTGCTCATGATGAAGACGCCTCTTGTTCTCGTCCCGACGCCCAGACTATCCGACCCTACTCTGTATCAGTAA

Protein sequence

MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNTVAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQFLLEVKDSGDSKTPLTITHLKALLMMKTPLVLVPTPRLSDPTLYQ
Homology
BLAST of Csor.00g308690 vs. ExPASy Swiss-Prot
Match: E9KBR8 (Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.8e-44
Identity = 97/302 (32.12%), Postives = 167/302 (55.30%), Query Frame = 0

Query: 33  FLFTVFFAGLLIFLYTKVTRRRVPL--PPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGP 92
           FL     A LL  L+ +  + +  L  PP P  +P++G+L  L P +H  FRDL  +YGP
Sbjct: 10  FLIVFISASLLKLLFVRENKPKAHLKNPPSPPAIPIIGHLHLLKPLIHHSFRDLSLRYGP 69

Query: 93  IVKLQLGNKVGIIVNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDEWR 152
           ++ L++G+   I+ ++PS+A+E LK N++T+++R +  A    TY  +  A+ PY   W+
Sbjct: 70  LLSLRIGSVKFIVASTPSLAQEFLKTNELTYSSRKMNMAINMVTYHNATFAFAPYDTYWK 129

Query: 153 MLRKVCVVKMLSNATLESVYDLRRREVRNTVAILY--GRAGSAMNVGEQSFLTIFNVVTS 212
            ++K+   ++L N TL     +R REV + +  L+   +A  ++N+ E       NV++ 
Sbjct: 130 FMKKLSTTELLGNKTLGHFLPIRTREVHDIIQFLFHKSKAQESVNLTEALLSLSNNVISQ 189

Query: 213 MLWGGTVKGDQRDSLAAEFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHK 272
           M+   ++K    DS A + R  V ++T++ G+ NVSDF       DLQG  K+   +  +
Sbjct: 190 MML--SIKSSGTDSQAEQARTLVREVTQIFGEFNVSDFLGFCKNLDLQGFRKRALDIHKR 249

Query: 273 FDTIFENMINQR------LKIAGGADGESAKNKDFLQFLLEVKDSGDSKTPLTITHLKAL 325
           +D + E +I+ R       K+ G  DG+  K KDFL  LL+V +  + +  LT  H+K+L
Sbjct: 250 YDALLEKIISDREELRRKSKVDGCEDGDDEKVKDFLDILLDVAEQKECEVQLTRNHVKSL 309

BLAST of Csor.00g308690 vs. ExPASy Swiss-Prot
Match: A6YIH8 (Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.2e-39
Identity = 101/296 (34.12%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 31  SRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPLGVPLLGNLPFLDPEL-HTYFRDLGQKYG 90
           S FLF  F    L+  +     +   LPPGP  +PLLG++  +   L H   RDL +KYG
Sbjct: 8   SIFLFLSFL--FLLRKWKNSNSQSKKLPPGPWKLPLLGSMLHMVGGLPHHVLRDLAKKYG 67

Query: 91  PIVKLQLGNKVGIIVNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDEW 150
           P++ LQLG    ++V SP +A+E+LK +D+ FA+R    A     Y  SDIA+ PYGD W
Sbjct: 68  PLMHLQLGEVSAVVVTSPDMAKEVLKTHDIAFASRPKLLAPEIVCYNRSDIAFCPYGDYW 127

Query: 151 RMLRKVCVVKMLSNATLESVYDLRRREVRNTVAILYGRAGSAMNVGEQSFLTIFNVVTSM 210
           R +RK+CV+++LS   + S   +RR EV   V  +       +N  E+ FL   ++    
Sbjct: 128 RQMRKICVLEVLSAKNVRSFSSIRRDEVLRLVNFVRSSTSEPVNFTERLFLFTSSMTCRS 187

Query: 211 LWGGTVKGDQRDSLAAEFRETVSDLTELLGKPNVSDFFPSLARFD-LQGIEKKMHKLAHK 270
            +G   K  +       F + + ++  L G  +V+D FPSL     L G+E K+ K  HK
Sbjct: 188 AFGKVFKEQE------TFIQLIKEVIGLAGGFDVADIFPSLKFLHVLTGMEGKIMKAHHK 247

Query: 271 FDTIFENMINQRLK-IAGGADGESAKNKDFLQFLLEVKDSGDSKTPLTITHLKALL 324
            D I E++IN+  K +A G    +   +D +  LL + + G  + P+T  ++KA++
Sbjct: 248 VDAIVEDVINEHKKNLAMGKTNGALGGEDLIDVLLRLMNDGGLQFPITNDNIKAII 295

BLAST of Csor.00g308690 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.3e-39
Identity = 97/298 (32.55%), Postives = 159/298 (53.36%), Query Frame = 0

Query: 33  FLFTVFFAGLLIF-LYTKVTRRRVPLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPI 92
           ++ T+  +  +++ LY + + +  PLPPGP G P+LGNLP L  + H     L ++YGP+
Sbjct: 8   WISTLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQYGPL 67

Query: 93  VKLQLGNKVGIIVNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDEWRM 152
            +L+LG+   ++ +S +VA + L+ +DV F+NR          Y   D+ + PYG  WRM
Sbjct: 68  FRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRM 127

Query: 153 LRKVCVVKMLSNATLESVYDLRRREVRNTVAIL--YGRAGSAMNVGEQSFLTIFNVVTSM 212
           LRK+C V + S   L+ +  +R+ EV   V  L  +   G  +N+G+   +   N +   
Sbjct: 128 LRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALARA 187

Query: 213 LWGGTVKGDQRDSLAA--EFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAH 272
           + G  V  D+   LA   EF+E V +L  L G  NV DF P L   DLQG+  KM +L  
Sbjct: 188 MLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHR 247

Query: 273 KFDTIFENMINQRLKIAGGADGESAKNKDFLQFLLEVKDS-GDSKTPLTITHLKALLM 325
           ++D   + +I +        +  +AK+ D L  L+ +K++  + +  L  T +KALL+
Sbjct: 248 RYDAFLDRVIEE--------NQANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLL 297

BLAST of Csor.00g308690 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-38
Identity = 90/294 (30.61%), Postives = 154/294 (52.38%), Query Frame = 0

Query: 36  TVFFAGLLIFLYTKVTRRRVPLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQ 95
           T+ FA +   L+ K  + +  LPP P+ +P++G+L  L P  H  F  L  +YGPI+ L 
Sbjct: 13  TIVFASI---LWRKQNKNKTLLPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLF 72

Query: 96  LGNKVGIIVNSPSVAREILKDNDVTFANRDV-PQAGRAATYGGSDIAWTPYGDEWRMLRK 155
           LG+   ++ ++   A+E LK ++  F+NR     A    TYG  D  + PYG  W+ ++K
Sbjct: 73  LGSVPCVVASTAEAAKEFLKTHEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKK 132

Query: 156 VCVVKMLSNATLESVYDLRRREVRNTV--AILYGRAGSAMNVGEQSFLTIFNVVTSMLWG 215
           +C+ ++L    L+    +R+ E +  +   +  G +G A++ G + F+T+ N + S +  
Sbjct: 133 LCMSELLGGHMLDQFLPVRQXETKKFIKRVLQKGISGEAVDFGGE-FITLSNNIVSRMIV 192

Query: 216 GTVKGDQRDSLAAEFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHKFDTI 275
                 + ++   E R+ V D  EL GK N+SDF   L RFDLQG  K++ K+   FDT+
Sbjct: 193 SQTSTTEDENEVEEMRKLVKDAAELSGKFNISDFVSFLKRFDLQGFNKRLEKIRDCFDTV 252

Query: 276 FENMINQRLKIAGGADGESAKN--KDFLQFLLEVKDSGDSKTPLTITHLKALLM 325
            + +I QR +     +    K   KD L  L ++ +   S+  L   ++KA ++
Sbjct: 253 LDRIIKQREEERRNKNETVGKREFKDMLDVLFDISEDESSEIKLNKENIKAFIL 302

BLAST of Csor.00g308690 vs. ExPASy Swiss-Prot
Match: C0SJS3 (Angelicin synthase (Fragment) OS=Pastinaca sativa OX=4041 GN=CYP71AJ4 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.8e-38
Identity = 96/296 (32.43%), Postives = 162/296 (54.73%), Query Frame = 0

Query: 33  FLFTVFFAGLLIFLYTKVTRRRVP---LPPGPLGVPLLGNLPFLDPELHTYFRDLGQKYG 92
           + F++F   + IFLY K   ++ P   LPP P  +P++GNL  + P+LH   RDL +KYG
Sbjct: 1   YFFSLFL--VTIFLY-KWLAKKTPSKNLPPSPPRLPIIGNLHQIGPDLHISLRDLARKYG 60

Query: 93  PIVKLQLGNKVGIIVNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDEW 152
           P+++LQLG    ++V+S    RE+LK +DV F+ R +  A     Y G D+A++ Y + W
Sbjct: 61  PLMQLQLGRIPVLVVSSAEATREVLKTHDVVFSQRPITSAIDKLCYKGRDVAFSRYSEYW 120

Query: 153 RMLRKVCVVKMLSNATLESVYDLRRREVRNTVAILYGRAGSAMNVGEQSFLTIFNVVTSM 212
           R +R  CV ++LSN+ + S +++R  EV   +  +   A   +N+GEQ      NVV  +
Sbjct: 121 RQVRSTCVTQLLSNSRVHSFHNIREEEVALLIQNIENSASEVINLGEQLIQLTRNVVCRV 180

Query: 213 LWGGTVKGDQRDSLAAEFRETVSDLTELLGKP-NVSDFFPSLARFD-LQGIEKKMHKLAH 272
             G       +  L   +++ ++++TE+L    ++ DF P L   D L G + K+ K A 
Sbjct: 181 SVGSEYLSGHKGKL---YQKLLAEVTEMLAYTYSIGDFIPLLGWVDWLSGSKAKVEKTAK 240

Query: 273 KFDTIFENMINQRLKIAGGADGESAKNKDFLQFLLEVKDSGDSKTPLTITHLKALL 324
           + D   E  +   +K    A  + + N DFL  LLE++++ D+ + L    +KA++
Sbjct: 241 EVDAFLEGALRDHIKTM--ASNKGSANDDFLSILLEIREA-DAGSTLDEECIKAIV 287

BLAST of Csor.00g308690 vs. NCBI nr
Match: KAG6579436.1 (Premnaspirodiene oxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693 bits (1788), Expect = 1.21e-251
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120
           PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180
           FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT
Sbjct: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180

Query: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240
           VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK
Sbjct: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300
           PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF
Sbjct: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300

Query: 301 LLEVKDSGDSKTPLTITHLKALLMMKTPLVLVPTPRLSDPTLYQ 344
           LLEVKDSGDSKTPLTITHLKALLMMKTPLVLVPTPRLSDPTLYQ
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLMMKTPLVLVPTPRLSDPTLYQ 344

BLAST of Csor.00g308690 vs. NCBI nr
Match: XP_022970206.1 (geraniol 8-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 627 bits (1618), Expect = 8.99e-223
Identity = 311/324 (95.99%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           MEPTSN LQ  IDSLNFL PWRWIDAADNQSR LFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   MEPTSNPLQIFIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120
           PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180
           FANRDVPQAGRAA+YGGSDIAWTPYG EWRMLRKVCV+KMLSNATLESVYDLRRREVRNT
Sbjct: 121 FANRDVPQAGRAASYGGSDIAWTPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNT 180

Query: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240
           VAILYGRAGSA+NVGEQSFLTIFNVVTSMLWGGTV+GDQRDSLAAEFRETVSDLTELLGK
Sbjct: 181 VAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300
           PNVSDFFP LARFDLQGIEKKMHKLAHKFDTIFENMINQRLKI+GG DGESAKNKDFLQF
Sbjct: 241 PNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKISGGEDGESAKNKDFLQF 300

Query: 301 LLEVKDSGDSKTPLTITHLKALLM 324
           LLEVKDSGDSKTPLTITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308690 vs. NCBI nr
Match: XP_023551603.1 (geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617 bits (1592), Expect = 8.09e-219
Identity = 308/324 (95.06%), Postives = 313/324 (96.60%), Query Frame = 0

Query: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           ME TSNSLQT IDSLNFL PWRWI AADNQSR LFTVFFAGLLIFLYTKVTRRRV LPPG
Sbjct: 1   METTSNSLQTLIDSLNFLLPWRWIHAADNQSRLLFTVFFAGLLIFLYTKVTRRRVTLPPG 60

Query: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120
           PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKD+DVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDHDVT 120

Query: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180
           FANRDVPQAGRAATYGGSDIAWTPYG EWRMLRKVCVVKMLSNATLESVYDLRRREVRNT
Sbjct: 121 FANRDVPQAGRAATYGGSDIAWTPYGAEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180

Query: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240
           VA LYGR GSA+NVGEQSFLTIFNVVTSMLWGG ++GDQRDSLAAEFRETVSDLTELLGK
Sbjct: 181 VANLYGRGGSAVNVGEQSFLTIFNVVTSMLWGGIMEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300
           PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGG DGESAK+KDFLQF
Sbjct: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKSKDFLQF 300

Query: 301 LLEVKDSGDSKTPLTITHLKALLM 324
           LLEVKDSGDSKTPLTITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308690 vs. NCBI nr
Match: XP_022970207.1 (geraniol 8-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 611 bits (1576), Expect = 2.19e-216
Identity = 305/324 (94.14%), Postives = 310/324 (95.68%), Query Frame = 0

Query: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           ME TSNSL+T IDSLNFL PW WID AD Q RFLFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   METTSNSLRTLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120
           PLGVPLLGNL FLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT
Sbjct: 61  PLGVPLLGNLLFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180
           FANRDVPQAGRAA YGGS+I W PYG EWRMLRKVCV+KMLSNATLESVYDLRRREVRNT
Sbjct: 121 FANRDVPQAGRAARYGGSNIVWNPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNT 180

Query: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240
           VAILYGRAGSA+NVGEQSFLTIFNVVTSMLWGGTV+GDQRDSLAAEFRETVSDLTELLGK
Sbjct: 181 VAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300
           PNVSDFFP LARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGG DGESAKNKDFLQF
Sbjct: 241 PNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQF 300

Query: 301 LLEVKDSGDSKTPLTITHLKALLM 324
           LLEVKDSGDSKTPLTITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308690 vs. NCBI nr
Match: KAG6579434.1 (Angelicin synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 595 bits (1534), Expect = 3.20e-213
Identity = 294/324 (90.74%), Postives = 305/324 (94.14%), Query Frame = 0

Query: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           ME TSNSLQT IDSLNFL PWRWIDAADNQSR LFTVFFAGLLIFLYTKV RRRVPLPPG
Sbjct: 1   METTSNSLQTLIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVKRRRVPLPPG 60

Query: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120
           PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKD+DVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDHDVT 120

Query: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180
           FANRDVPQAGRAATYGGS+I W PYG EWRMLRKVCVVKMLSNATLESVYDLRRREVRNT
Sbjct: 121 FANRDVPQAGRAATYGGSNIVWNPYGSEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180

Query: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240
           VA LYGRAGSA+NVGEQ FLT+FNVVT MLWGG ++GDQRDSLAAEFRETVSD TELLGK
Sbjct: 181 VANLYGRAGSAVNVGEQGFLTVFNVVTGMLWGGIMEGDQRDSLAAEFRETVSDFTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300
           PNVSDFFPSLARFDLQGIEKKM KLA KFDTIFENMINQRLKI GG DG+SA+N DFLQF
Sbjct: 241 PNVSDFFPSLARFDLQGIEKKMRKLALKFDTIFENMINQRLKIVGGEDGQSAQNHDFLQF 300

Query: 301 LLEVKDSGDSKTPLTITHLKALLM 324
           LLE+KD+GDSKTPLT+ HLKALLM
Sbjct: 301 LLEMKDAGDSKTPLTMIHLKALLM 324

BLAST of Csor.00g308690 vs. ExPASy TrEMBL
Match: A0A6J1I001 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469168 PE=3 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 4.35e-223
Identity = 311/324 (95.99%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           MEPTSN LQ  IDSLNFL PWRWIDAADNQSR LFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   MEPTSNPLQIFIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120
           PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180
           FANRDVPQAGRAA+YGGSDIAWTPYG EWRMLRKVCV+KMLSNATLESVYDLRRREVRNT
Sbjct: 121 FANRDVPQAGRAASYGGSDIAWTPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNT 180

Query: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240
           VAILYGRAGSA+NVGEQSFLTIFNVVTSMLWGGTV+GDQRDSLAAEFRETVSDLTELLGK
Sbjct: 181 VAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300
           PNVSDFFP LARFDLQGIEKKMHKLAHKFDTIFENMINQRLKI+GG DGESAKNKDFLQF
Sbjct: 241 PNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKISGGEDGESAKNKDFLQF 300

Query: 301 LLEVKDSGDSKTPLTITHLKALLM 324
           LLEVKDSGDSKTPLTITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308690 vs. ExPASy TrEMBL
Match: A0A6J1HYH5 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469169 PE=3 SV=1)

HSP 1 Score: 611 bits (1576), Expect = 1.06e-216
Identity = 305/324 (94.14%), Postives = 310/324 (95.68%), Query Frame = 0

Query: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           ME TSNSL+T IDSLNFL PW WID AD Q RFLFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   METTSNSLRTLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120
           PLGVPLLGNL FLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT
Sbjct: 61  PLGVPLLGNLLFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180
           FANRDVPQAGRAA YGGS+I W PYG EWRMLRKVCV+KMLSNATLESVYDLRRREVRNT
Sbjct: 121 FANRDVPQAGRAARYGGSNIVWNPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNT 180

Query: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240
           VAILYGRAGSA+NVGEQSFLTIFNVVTSMLWGGTV+GDQRDSLAAEFRETVSDLTELLGK
Sbjct: 181 VAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300
           PNVSDFFP LARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGG DGESAKNKDFLQF
Sbjct: 241 PNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQF 300

Query: 301 LLEVKDSGDSKTPLTITHLKALLM 324
           LLEVKDSGDSKTPLTITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308690 vs. ExPASy TrEMBL
Match: A0A6J1E5N3 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111430178 PE=3 SV=1)

HSP 1 Score: 597 bits (1540), Expect = 3.16e-211
Identity = 295/324 (91.05%), Postives = 306/324 (94.44%), Query Frame = 0

Query: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           ME TSNSLQT IDSLNFL PWRWIDAADNQSR LFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   METTSNSLQTLIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120
           PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKD+DVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDHDVT 120

Query: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180
           FANRDVPQAGRAATYGGS+I W PYG EWRMLRKVCVVKMLSNATLESVYDLRRREVRNT
Sbjct: 121 FANRDVPQAGRAATYGGSNIVWNPYGSEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180

Query: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240
           VA LYGRAGSA+NVGEQ FLT+FNVVT MLWGG ++GDQRDSLAAEFRETVSD TELLGK
Sbjct: 181 VANLYGRAGSAVNVGEQGFLTVFNVVTGMLWGGIMEGDQRDSLAAEFRETVSDFTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300
           PNVSDFFPSLARFDLQGIEKKM KLA KFDTIFENMINQRLKI GG DG+SA+N DFLQF
Sbjct: 241 PNVSDFFPSLARFDLQGIEKKMRKLALKFDTIFENMINQRLKIVGGEDGQSAQNHDFLQF 300

Query: 301 LLEVKDSGDSKTPLTITHLKALLM 324
           LLE+KD+GDSKTPLT+ HLKALLM
Sbjct: 301 LLEMKDAGDSKTPLTMIHLKALLM 324

BLAST of Csor.00g308690 vs. ExPASy TrEMBL
Match: A0A0A0KR55 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159600 PE=3 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 7.59e-167
Identity = 237/296 (80.07%), Postives = 265/296 (89.53%), Query Frame = 0

Query: 29  NQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKY 88
           +QS F FT F A LLIFLY ++TR RVPLPPGP GVPLLGNLPFL PELHTYF  LGQKY
Sbjct: 12  DQSHFFFT-FLAALLIFLYLRLTRLRVPLPPGPRGVPLLGNLPFLHPELHTYFAQLGQKY 71

Query: 89  GPIVKLQLGNKVGIIVNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDE 148
           GPIVKLQLG K+GII+NSPSV RE+LKD+DVTFANRDVPQAGRAA+YGGSDI WTPYG +
Sbjct: 72  GPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPQAGRAASYGGSDIVWTPYGPK 131

Query: 149 WRMLRKVCVVKMLSNATLESVYDLRRREVRNTVAILYGRAGSAMNVGEQSFLTIFNVVTS 208
           WRMLRKVCVVKMLSNATL+SVY+LRR+EVRNTVA LY RAG+A+NVGEQ FLT+FNVVTS
Sbjct: 132 WRMLRKVCVVKMLSNATLDSVYELRRKEVRNTVAHLYARAGTAVNVGEQGFLTVFNVVTS 191

Query: 209 MLWGGTVKGDQRDSLAAEFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHK 268
           MLWGG+V+G+QRD LAAEFRETVS++TELLG PNVSDFFPSLARFDLQGIEKKM +LA +
Sbjct: 192 MLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDLQGIEKKMRELAPR 251

Query: 269 FDTIFENMINQRLKIAGGADGESAKNKDFLQFLLEVKDSGDSKTPLTITHLKALLM 324
           FD+IFE MI+QRLKI GG +G S K  DFLQFLL+VKD  +SKTPLT+THLKALLM
Sbjct: 252 FDSIFEKMIDQRLKIDGGDEGGSVKKNDFLQFLLQVKDEEESKTPLTMTHLKALLM 306

BLAST of Csor.00g308690 vs. ExPASy TrEMBL
Match: A0A1S3ASY0 (flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482651 PE=3 SV=1)

HSP 1 Score: 483 bits (1243), Expect = 2.16e-166
Identity = 235/296 (79.39%), Postives = 264/296 (89.19%), Query Frame = 0

Query: 29  NQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKY 88
           +QS F FT F A LLIFLY ++TR RVPLPPGP GVPLLGNLPFLDPELHTYF  L QKY
Sbjct: 12  DQSHFFFT-FLAALLIFLYLRLTRLRVPLPPGPRGVPLLGNLPFLDPELHTYFAQLSQKY 71

Query: 89  GPIVKLQLGNKVGIIVNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDE 148
           GPIVKLQLG K+GII+NSPSV RE+LKD+DVTFANRDVP AGRAA+YGGSDI WTPYG +
Sbjct: 72  GPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPHAGRAASYGGSDIVWTPYGPQ 131

Query: 149 WRMLRKVCVVKMLSNATLESVYDLRRREVRNTVAILYGRAGSAMNVGEQSFLTIFNVVTS 208
           WRMLRKVCV+KMLSNATL+SVY+LRRREVRNTVA LY RAG+ +NVGEQ FLT+FNVVTS
Sbjct: 132 WRMLRKVCVLKMLSNATLDSVYELRRREVRNTVAHLYARAGTRVNVGEQGFLTVFNVVTS 191

Query: 209 MLWGGTVKGDQRDSLAAEFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHK 268
           MLWGG+V+G+QRD LAAEFRETVS++TELLG PNVSDFFPSLARFD+QGIEKKM +LA +
Sbjct: 192 MLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDIQGIEKKMRELAPR 251

Query: 269 FDTIFENMINQRLKIAGGADGESAKNKDFLQFLLEVKDSGDSKTPLTITHLKALLM 324
           FD+IFE MI+QRLKI G  +GES K  DFLQFLL+VKD G+SKTPLT+THLKALLM
Sbjct: 252 FDSIFEKMIDQRLKIDGKDEGESVKKNDFLQFLLQVKDDGESKTPLTMTHLKALLM 306

BLAST of Csor.00g308690 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 328.9 bits (842), Expect = 4.7e-90
Identity = 156/299 (52.17%), Postives = 212/299 (70.90%), Query Frame = 0

Query: 29  NQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKY 88
           N + +   +  A   I  Y      +  LPPGP G+P++GNLPFLDP+LHTYF  L + Y
Sbjct: 14  NLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFLDPDLHTYFTKLAESY 73

Query: 89  GPIVKLQLGNKVGIIVNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDE 148
           GPI KL LG+K+ ++VN+PS+AREILKD D+ F+N DVP   RA TYGG D+ W PYG E
Sbjct: 74  GPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAE 133

Query: 149 WRMLRKVCVVKMLSNATLESVYDLRRREVRNTVAILY--GRAGSAMNVGEQSFLTIFNVV 208
           WRMLRKVCV+K+LS+ TL S Y+LRR+E+R     LY  G+  S +NVGEQ FLT+ N+ 
Sbjct: 134 WRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMNLT 193

Query: 209 TSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLA 268
            +MLWGG+VK ++ +S+  EF+E +S++T LLG+PNVSDFFP LARFDLQG+ KKMH  A
Sbjct: 194 MNMLWGGSVKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKMHVCA 253

Query: 269 HKFDTIFENMINQRLKIAGGADGESAKNKDFLQFLLEVKD-SGDSKTPLTITHLKALLM 325
            + D I +  I Q +++    DG+  + KDFLQ L+++KD   DS+ P+T+ H+KA+L+
Sbjct: 254 RELDAILDRAIEQ-MQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVKAVLV 311

BLAST of Csor.00g308690 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 327.4 bits (838), Expect = 1.4e-89
Identity = 156/270 (57.78%), Postives = 202/270 (74.81%), Query Frame = 0

Query: 57  LPPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKD 116
           LPPGP G+P++GNLPFLDP+LHTYF +L Q +GPI KL LG+K+ I+VNSPS+AREILKD
Sbjct: 42  LPPGPRGLPIVGNLPFLDPDLHTYFANLAQSHGPIFKLNLGSKLTIVVNSPSLAREILKD 101

Query: 117 NDVTFANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRRE 176
            D+ F+NRDVP  GRAATYGG DI WTPYG EWR LRK+CV+K+LS  TL+S Y+LRR+E
Sbjct: 102 QDINFSNRDVPLTGRAATYGGIDIVWTPYGAEWRQLRKICVLKLLSRKTLDSFYELRRKE 161

Query: 177 VRNTVAILY--GRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDL 236
           VR     LY  GR  S + VG+Q FLT+ N+  +MLWGG+VK ++ +S+  EF+  +S++
Sbjct: 162 VRERTRYLYEQGRKQSPVKVGDQLFLTMMNLTMNMLWGGSVKAEEMESVGTEFKGVISEI 221

Query: 237 TELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKN 296
           T LL +PNVSDFFP LARFDLQG+ K+M   A + D + +  I Q   + G  D E    
Sbjct: 222 TRLLSEPNVSDFFPWLARFDLQGLVKRMGVCARELDAVLDRAIEQMKPLRGRDDDEV--- 281

Query: 297 KDFLQFLLEVKD-SGDSKTPLTITHLKALL 324
           KDFLQ+L+++KD  GDS+ P+TI H+KALL
Sbjct: 282 KDFLQYLMKLKDQEGDSEVPITINHVKALL 308

BLAST of Csor.00g308690 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 319.3 bits (817), Expect = 3.7e-87
Identity = 154/274 (56.20%), Postives = 196/274 (71.53%), Query Frame = 0

Query: 56  PLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILK 115
           PLPPGP G+P++GNLPFLDP+LHTYF  L Q +GPI KL LG+K+ ++VNSPS+A EILK
Sbjct: 43  PLPPGPRGLPIVGNLPFLDPDLHTYFTKLAQSHGPIFKLNLGSKLTVVVNSPSLASEILK 102

Query: 116 DNDVTFANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRR 175
           D D+ F+N DVP   RA TYGG D+ W PYG EWRMLRKVC  K+ S  TL+S Y+LRR+
Sbjct: 103 DQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRKVCAAKLFSRKTLDSFYELRRK 162

Query: 176 EVRNTVAILY--GRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSD 235
           E+R     LY  G   S +NVGEQ FLT+ N++ +MLWGG+VK +  +S+  EF+  +S+
Sbjct: 163 EIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNMLWGGSVKAEDMESVGTEFKGVISE 222

Query: 236 LTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMIN--QRLKIAGGADGES 295
           +T LLG PNVSDFFP LARFDLQG+ KKMH  A   D I +  I   QRL+   G DGE 
Sbjct: 223 ITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYARDLDAILDRAIEQMQRLRSRDGDDGEC 282

Query: 296 AKNKDFLQFLLEVKD-SGDSKTPLTITHLKALLM 325
              KDFLQ L++++D   DS  P+T+ H+KA+LM
Sbjct: 283 ---KDFLQHLMKLRDQEADSDVPITMNHVKAVLM 313

BLAST of Csor.00g308690 vs. TAIR 10
Match: AT5G44620.1 (cytochrome P450, family 706, subfamily A, polypeptide 3 )

HSP 1 Score: 312.8 bits (800), Expect = 3.5e-85
Identity = 156/285 (54.74%), Postives = 203/285 (71.23%), Query Frame = 0

Query: 44  IFLYTKVTRRRVPLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGII 103
           ++LY K  RR  PLPPGP G+P++GNLPFL PELHTYF+ L +K+GPI KL LG K+ I+
Sbjct: 33  LWLYAKCKRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQGLAKKHGPIFKLWLGAKLTIV 92

Query: 104 VNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSN 163
           V S  VA+EILK ND+ FAN DVP  G   TYGG++I W+PYG +WRMLRK+CV ++L N
Sbjct: 93  VTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGPKWRMLRKLCVNRILRN 152

Query: 164 ATLESVYDLRRREVRNTVAIL--YGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRD 223
           A L+S  DLRRRE R TV  L    R GS +N+GEQ FL + NVVT MLWG TVK ++R+
Sbjct: 153 AMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNVVTQMLWGTTVKEEERE 212

Query: 224 SLAAEFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRL 283
            + AEF E + ++ +LL  PN+SDFFP L+RFDLQG+ K+M + A + D +F+ +INQRL
Sbjct: 213 VVGAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKRMRRPAQRMDQMFDRIINQRL 272

Query: 284 KIAGGADGESAKNK--DFLQFLLEVKDSGDSKTPLTITHLKALLM 325
               G D +S+  +  DFL  LL+VKD    KT LT+  +KA+LM
Sbjct: 273 ----GMDRDSSDGRAVDFLDVLLKVKDEEAEKTKLTMNDVKAVLM 313

BLAST of Csor.00g308690 vs. TAIR 10
Match: AT4G12330.1 (cytochrome P450, family 706, subfamily A, polypeptide 7 )

HSP 1 Score: 301.2 bits (770), Expect = 1.1e-81
Identity = 152/286 (53.15%), Postives = 201/286 (70.28%), Query Frame = 0

Query: 44  IFLYTKVTRRRVPLPPGPLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGII 103
           I++Y K  R   PLPPGP G+P++GNLPFL PELHTYF  L QK+GP+ KL LG K+ I+
Sbjct: 33  IWVYVKSKRLFPPLPPGPRGLPIVGNLPFLHPELHTYFHSLAQKHGPVFKLWLGAKLTIV 92

Query: 104 VNSPSVAREILKDNDVTFANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSN 163
           + S    R+IL+ NDV FAN DVP AG  +TYGG DI W+PYG EW MLRK+C+ KMLSN
Sbjct: 93  ITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGVDIVWSPYGPEWPMLRKICINKMLSN 152

Query: 164 ATLE--SVYDLRRREVRNTVAIL--YGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGD- 223
           ATL+  S   LRR+E R TV  L    RAG A+NVGEQ F+TI NVVT MLWG TV  D 
Sbjct: 153 ATLDSNSFSALRRQETRRTVRYLADRARAGLAVNVGEQIFVTILNVVTQMLWGETVADDE 212

Query: 224 QRDSLAAEFRETVSDLTELLGKPNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMIN 283
           +R+ + AEF E ++++ +++GKPNVSDFFP L+RFDLQG+ K++ + A + D +F+ +I+
Sbjct: 213 EREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRFDLQGLAKRVRRSAQRMDRMFDRIIS 272

Query: 284 QRLKIAGGADGESAKNKDFLQFLLEVKDSGDSKTPLTITHLKALLM 325
           QR+ +  G+ G      DFL  LL  KD  ++   +++ H+KALLM
Sbjct: 273 QRMGMDKGSKGNGG---DFLMVLLNAKDEDEN---MSMNHVKALLM 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E9KBR83.8e-4432.12Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1[more]
A6YIH82.2e-3934.12Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1[more]
A0A4D6Q4156.3e-3932.55Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
O819731.1e-3830.61Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1[more]
C0SJS31.8e-3832.43Angelicin synthase (Fragment) OS=Pastinaca sativa OX=4041 GN=CYP71AJ4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6579436.11.21e-251100.00Premnaspirodiene oxygenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022970206.18.99e-22395.99geraniol 8-hydroxylase-like [Cucurbita maxima][more]
XP_023551603.18.09e-21995.06geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_022970207.12.19e-21694.14geraniol 8-hydroxylase-like [Cucurbita maxima][more]
KAG6579434.13.20e-21390.74Angelicin synthase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1I0014.35e-22395.99geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469168 PE=3 SV=... [more]
A0A6J1HYH51.06e-21694.14geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469169 PE=3 SV=... [more]
A0A6J1E5N33.16e-21191.05geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111430178 PE=3 S... [more]
A0A0A0KR557.59e-16780.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159600 PE=3 SV=1[more]
A0A1S3ASY02.16e-16679.39flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482651 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT4G12320.14.7e-9052.17cytochrome P450, family 706, subfamily A, polypeptide 6 [more]
AT4G12300.11.4e-8957.78cytochrome P450, family 706, subfamily A, polypeptide 4 [more]
AT4G12310.13.7e-8756.20cytochrome P450, family 706, subfamily A, polypeptide 5 [more]
AT5G44620.13.5e-8554.74cytochrome P450, family 706, subfamily A, polypeptide 3 [more]
AT4G12330.11.1e-8153.15cytochrome P450, family 706, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 85..104
score: 27.83
coord: 109..130
score: 24.15
coord: 201..219
score: 17.64
IPR001128Cytochrome P450PFAMPF00067p450coord: 58..324
e-value: 5.4E-30
score: 104.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 50..325
e-value: 1.1E-45
score: 158.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 56..324
NoneNo IPR availablePANTHERPTHR47951:SF3CYTOCHROME P450, FAMILY 706, SUBFAMILY A, POLYPEPTIDE 3-RELATEDcoord: 30..324
NoneNo IPR availablePANTHERPTHR47951OS08G0547900 PROTEINcoord: 30..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g308690.m01Csor.00g308690.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen