Csor.00g308680 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g308680
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionCytochrome P450, putative
LocationCsor_Chr15: 9223541 .. 9225353 (+)
RNA-Seq ExpressionCsor.00g308680
SyntenyCsor.00g308680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAACCACCTCCGATTCTCTTCGACCTCTCATAGATTCCCTCAATTTCTTGCTCCCATGGCCGTGGATTGACACCGCCGATTACCAGTTCCGCTTCCTCTTCACCGTCTTCTTCGCCGGTCTATTAATTTTCCTCTATACTAAAGTTACGCGTCGGCGCGTGCCACTGCCACCAGGTCCTCCGGGAGTTCCCCTGCTCGGAAACCTGCCGTTTCTCGACCCCGAACTTCACACCTACTTAAGAGAATTGGGCCGTAAATACGGGCCGATAGTAAAGATCCAACTCGGTAGAAAGGTGGGTATAGTCGTCAATTCACCCTCTGTGGCACGTGAGATTTTGAAAGATCACGATGTCACGTTCGCCAATCGTGACGTTCCTCAGGCTGGGAGACTCGCCTCGTACGGCAGTTCTGACATTGTTTGGACCCCGTACGGAGCAGAGTGGCGCATGTTGAGAAAAGTTTGCGTGCTCAAGATGCTCAGCAACGCCGCTTTGGAGTCCGTATACAAGCTCCTTCGTAGAGAGGTCAGAAACACCGTCGCTAACCTCTACCGGCGAAGCGGGTCCGCGGTGAATGTGGGAGAGCAGAGTTTTTTGACGATTTTCAACGCGGCTACGAGCATGTTGTGGGGTGGGACAGTGGAGGGGGACCAGAGGGATAGCCTTGCAGCTGAGTTCAGAAAGACAGTTTCCGACATGACTGGGCTACAGGGAAGGCCTAATGTCTCTGACTTTTTTCCAAGCTTGGCTCGTTTCGATCTCCAGGGCATTGAAAAGCAGATGCACAAGCTTGCTCACAAATTCGATACCATTTTCGAGAATATGATCAATCAACGATTGAAAATTGCCGGTGGGGAAGACGGCGAGAGTGCCAAGAATAAAGATTTTTTGCAGGTCTTACTTGAGGTGAAAGATGCTGGCGACTCCAAAACTCCTTTCACCATCACCCACCTCAAAGCCTTGCTCATGGTAACTAATTTTAATCCCCTTTTTTTATGTTATATTACTAGAAATTTGAGGAATGGAAATGGGTTGTGTTGGTAGGATATGGTGATTGGGGGGACAGACACATCGGCAAATACCATAGAGTTTGCGCTGGCAGAGCTGGTAAAGAACCCTGCAACTCTGAAGAAAGCACAGGAAGAAGTAGCGGCCATGGTGGGAGAAGACAACATCGTGGAGGAGTCCCACATTCATAATTTGCCATATTTAAAAGCCGTAATGAAAGAAACTTTGCGTTTGCACCCAATGATACCGCTGCTAGTGCCACACTGCCCAAGTGAGACCACCATCATCTCCAAATACACAATCCCAAAGGGCTCTCGAGTGTTCATCAACGTGTGGGCCATTCAACGAGACCCCAACCACTGGGAAAATCCATTGGTGTTCGATCCGGAGAGGTTTGTTGACAATCAAAAGTGGGATTTCGGTGGGAGTGATTTACGTTACTTCCCATTTGGGTCCGGCAGAAGAAACTGTGTAGGGATAGCCCTGGCTGAAAGAATGTTCATGTTCTTGCTTGCTACGCTCTTGCATTCCTTTGATTGGAAGGTGGAAGAGGGTGAGAAACTGGAAGTTGAGGAGAAATTTGGGATTGACGCCTCTTGTTCTCATCCCAACGCCAAGGCTATCTGACTCCACTCTGTATCAGTAAGCTTAAAAAGTTAAATTTGTTAGTGCTTTATCTGCCTAAATACTCATAAGATCTATATACAACTGAAAAACATAATTGCTCACGGCTCAGGGCTCCTCGGTTCAAAGAACTCTTGTTTCTATTTATTGTATTTGTTTCGAAATATTTCAATGGTGA

mRNA sequence

ATGGAAACCACCTCCGATTCTCTTCGACCTCTCATAGATTCCCTCAATTTCTTGCTCCCATGGCCGTGGATTGACACCGCCGATTACCAGTTCCGCTTCCTCTTCACCGTCTTCTTCGCCGGTCTATTAATTTTCCTCTATACTAAAGTTACGCGTCGGCGCGTGCCACTGCCACCAGGTCCTCCGGGAGTTCCCCTGCTCGGAAACCTGCCGTTTCTCGACCCCGAACTTCACACCTACTTAAGAGAATTGGGCCGTAAATACGGGCCGATAGTAAAGATCCAACTCGGTAGAAAGGTGGGTATAGTCGTCAATTCACCCTCTGTGGCACGTGAGATTTTGAAAGATCACGATGTCACGTTCGCCAATCGTGACGTTCCTCAGGCTGGGAGACTCGCCTCGTACGGCAGTTCTGACATTGTTTGGACCCCGTACGGAGCAGAGTGGCGCATGTTGAGAAAAGTTTGCGTGCTCAAGATGCTCAGCAACGCCGCTTTGGAGTCCGTATACAAGCTCCTTCGTAGAGAGGTCAGAAACACCGTCGCTAACCTCTACCGGCGAAGCGGGTCCGCGGTGAATGTGGGAGAGCAGAGTTTTTTGACGATTTTCAACGCGGCTACGAGCATGTTGTGGGGTGGGACAGTGGAGGGGGACCAGAGGGATAGCCTTGCAGCTGAGTTCAGAAAGACAGTTTCCGACATGACTGGGCTACAGGGAAGGCCTAATGTCTCTGACTTTTTTCCAAGCTTGGCTCGTTTCGATCTCCAGGGCATTGAAAAGCAGATGCACAAGCTTGCTCACAAATTCGATACCATTTTCGAGAATATGATCAATCAACGATTGAAAATTGCCGGTGGGGAAGACGGCGAGAGTGCCAAGAATAAAGATTTTTTGCAGGTCTTACTTGAGGTGAAAGATGCTGGCGACTCCAAAACTCCTTTCACCATCACCCACCTCAAAGCCTTGCTCATGGGCTCCTCGGTTCAAAGAACTCTTGTTTCTATTTATTGTATTTGTTTCGAAATATTTCAATGGTGA

Coding sequence (CDS)

ATGGAAACCACCTCCGATTCTCTTCGACCTCTCATAGATTCCCTCAATTTCTTGCTCCCATGGCCGTGGATTGACACCGCCGATTACCAGTTCCGCTTCCTCTTCACCGTCTTCTTCGCCGGTCTATTAATTTTCCTCTATACTAAAGTTACGCGTCGGCGCGTGCCACTGCCACCAGGTCCTCCGGGAGTTCCCCTGCTCGGAAACCTGCCGTTTCTCGACCCCGAACTTCACACCTACTTAAGAGAATTGGGCCGTAAATACGGGCCGATAGTAAAGATCCAACTCGGTAGAAAGGTGGGTATAGTCGTCAATTCACCCTCTGTGGCACGTGAGATTTTGAAAGATCACGATGTCACGTTCGCCAATCGTGACGTTCCTCAGGCTGGGAGACTCGCCTCGTACGGCAGTTCTGACATTGTTTGGACCCCGTACGGAGCAGAGTGGCGCATGTTGAGAAAAGTTTGCGTGCTCAAGATGCTCAGCAACGCCGCTTTGGAGTCCGTATACAAGCTCCTTCGTAGAGAGGTCAGAAACACCGTCGCTAACCTCTACCGGCGAAGCGGGTCCGCGGTGAATGTGGGAGAGCAGAGTTTTTTGACGATTTTCAACGCGGCTACGAGCATGTTGTGGGGTGGGACAGTGGAGGGGGACCAGAGGGATAGCCTTGCAGCTGAGTTCAGAAAGACAGTTTCCGACATGACTGGGCTACAGGGAAGGCCTAATGTCTCTGACTTTTTTCCAAGCTTGGCTCGTTTCGATCTCCAGGGCATTGAAAAGCAGATGCACAAGCTTGCTCACAAATTCGATACCATTTTCGAGAATATGATCAATCAACGATTGAAAATTGCCGGTGGGGAAGACGGCGAGAGTGCCAAGAATAAAGATTTTTTGCAGGTCTTACTTGAGGTGAAAGATGCTGGCGACTCCAAAACTCCTTTCACCATCACCCACCTCAAAGCCTTGCTCATGGGCTCCTCGGTTCAAAGAACTCTTGTTTCTATTTATTGTATTTGTTTCGAAATATTTCAATGGTGA

Protein sequence

METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNTVANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQVLLEVKDAGDSKTPFTITHLKALLMGSSVQRTLVSIYCICFEIFQW
Homology
BLAST of Csor.00g308680 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 6.8e-41
Identity = 103/300 (34.33%), Postives = 164/300 (54.67%), Query Frame = 0

Query: 31  FRFLFTVFFAGLLIF-LYTKVTRRRVPLPPGPPGVPLLGNLPFLDPELHTYLRELGRKYG 90
           F ++ T+  +  +++ LY + + +  PLPPGP G P+LGNLP L  + H  L  L ++YG
Sbjct: 6   FLWISTLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQYG 65

Query: 91  PIVKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEW 150
           P+ +++LG    +V +S +VA + L+ HDV F+NR         +Y   D+V+ PYG  W
Sbjct: 66  PLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRW 125

Query: 151 RMLRKVCVLKMLSNAALESVYKLLRREVRNTVANLYRR--SGSAVNVGEQSFLTIFNAAT 210
           RMLRK+C + + S  AL+ +  + + EV   V NL R   +G  VN+G+   +   NA  
Sbjct: 126 RMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALA 185

Query: 211 SMLWGGTVEGDQRDSLAA--EFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKL 270
             + G  V  D+   LA   EF++ V ++  L G  NV DF P L   DLQG+  +M +L
Sbjct: 186 RAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRL 245

Query: 271 AHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQVLLEVKDA-GDSKTPFTITHLKALLM 325
             ++D   + +I         E+  +AK+ D L VL+ +K+A  + +     T +KALL+
Sbjct: 246 HRRYDAFLDRVIE--------ENQANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLL 297

BLAST of Csor.00g308680 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.3e-39
Identity = 101/280 (36.07%), Postives = 146/280 (52.14%), Query Frame = 0

Query: 52  RRRVPLPPGPPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAR 111
           R+R PLPPGP G P+LGNLP L  + H  +  L R+YGP+ +++ G    +V  S  VA 
Sbjct: 36  RKRRPLPPGPRGWPVLGNLPQLGDKPHHTMCALARQYGPLFRLRFGCAEVVVAASAPVAA 95

Query: 112 EILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYK 171
           + L+ HD  F+NR         +Y   D+V+ PYGA WR LRK+C L + S  AL+ +  
Sbjct: 96  QFLRGHDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLRA 155

Query: 172 LLRREVRNTVANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTV 231
           +   EV   V NL R+  ++V +G+++ +   N       G  V        A EF++ V
Sbjct: 156 VREGEVALMVRNLARQQAASVALGQEANVCATNTLARATIGHRVFAVDGGEGAREFKEMV 215

Query: 232 SDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGES 291
            ++  L G  NV DF P+L   D QG+  +M +L  ++D +    IN+R K     DG +
Sbjct: 216 VELMQLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMMNGFINER-KAGAQPDGVA 275

Query: 292 A--KNKDFLQVLL-----EVKDAGDSKTPFTITHLKALLM 325
           A     D L VLL     E K  GD +   T T +KALL+
Sbjct: 276 AGEHGNDLLSVLLARMQEEQKLDGDGE-KITETDIKALLL 313

BLAST of Csor.00g308680 vs. ExPASy Swiss-Prot
Match: E9KBR8 (Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 8.3e-39
Identity = 93/323 (28.79%), Postives = 173/323 (53.56%), Query Frame = 0

Query: 33  FLFTVFFAGLLIFLYTKVTRRRVPL--PPGPPGVPLLGNLPFLDPELHTYLRELGRKYGP 92
           FL     A LL  L+ +  + +  L  PP PP +P++G+L  L P +H   R+L  +YGP
Sbjct: 10  FLIVFISASLLKLLFVRENKPKAHLKNPPSPPAIPIIGHLHLLKPLIHHSFRDLSLRYGP 69

Query: 93  IVKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEWR 152
           ++ +++G    IV ++PS+A+E LK +++T+++R +  A  + +Y ++   + PY   W+
Sbjct: 70  LLSLRIGSVKFIVASTPSLAQEFLKTNELTYSSRKMNMAINMVTYHNATFAFAPYDTYWK 129

Query: 153 MLRKVCVLKMLSNAALESVYKLLRREVRNTVANLYRRSGSAVNVG-EQSFLTIFNAATSM 212
            ++K+   ++L N  L     +  REV + +  L+ +S +  +V   ++ L++ N   S 
Sbjct: 130 FMKKLSTTELLGNKTLGHFLPIRTREVHDIIQFLFHKSKAQESVNLTEALLSLSNNVISQ 189

Query: 213 LWGGTVEGDQRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHKF 272
           +   +++    DS A + R  V ++T + G  NVSDF       DLQG  K+   +  ++
Sbjct: 190 MM-LSIKSSGTDSQAEQARTLVREVTQIFGEFNVSDFLGFCKNLDLQGFRKRALDIHKRY 249

Query: 273 DTIFENMINQR------LKIAGGEDGESAKNKDFLQVLLEVKDAGDSKTPFTITHLKALL 332
           D + E +I+ R       K+ G EDG+  K KDFL +LL+V +  + +   T  H+K+L+
Sbjct: 250 DALLEKIISDREELRRKSKVDGCEDGDDEKVKDFLDILLDVAEQKECEVQLTRNHVKSLI 309

Query: 333 MG---SSVQRTLVSIYCICFEIF 344
           +    ++   T +S+     E+F
Sbjct: 310 LDYFTAATDTTAISVEWTIAELF 331

BLAST of Csor.00g308680 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.1e-38
Identity = 101/305 (33.11%), Postives = 152/305 (49.84%), Query Frame = 0

Query: 34  LFTVFFAGLLIFLYTKVTRRR--VPLPPGPPGVPLLGNLPFLDPELHTYLRELGRKYGPI 93
           L+TV F+ LL F+     R+R  +PLPPGP   P++GNL  L P+ H     + + YGP+
Sbjct: 8   LYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMAQTYGPL 67

Query: 94  VKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEWRM 153
           + +++G    +V  S SVA + LK HD  F++R         +Y   D+V+ PYG  WRM
Sbjct: 68  MYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPRWRM 127

Query: 154 LRKVCVLKMLSNAALESVYKLLRREVRNTVANLYRRSGSAVNVGEQSFLTIFNAATSMLW 213
           LRK+C + + S  AL+    + + EV+     L       V +G+   +   NA   ++ 
Sbjct: 128 LRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALARVML 187

Query: 214 GGTVEGD---QRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHK 273
           G  V  D     D  AAEF+  V +M  + G  N+ DF P L   D+QG+  +M KL  +
Sbjct: 188 GKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKKLHAR 247

Query: 274 FDTIFENMINQRLKIAGGEDGESAKNKDFLQVLLEVK--DAGDSKTPFTITHLKALLMGS 332
           FD    +++ +      GE       KD L  L+ +K  DA +     T T +KALL+  
Sbjct: 248 FDAFLTDILEEHKGKIFGE------MKDLLSTLISLKNDDADNDGGKLTDTEIKALLLNL 306

BLAST of Csor.00g308680 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.4e-38
Identity = 99/293 (33.79%), Postives = 146/293 (49.83%), Query Frame = 0

Query: 34  LFTVFFAGLLIFLYTKVTRRRVPLPPGPPGVPLLGNLPFLDPELHTYLRELGRKYGPIVK 93
           L TV F  L IF + +       LPPGP   P++GNLP +  + H  L  +   YGPI+ 
Sbjct: 10  LATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILH 69

Query: 94  IQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEWRMLR 153
           ++LG    +V  S SVA + LK HD  FA+R      +  +Y   D+V+ PYG  WR+LR
Sbjct: 70  LRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLR 129

Query: 154 KVCVLKMLSNAALESVYKLLRREVRNTVANLYRRSGSAVNVGEQSFLTIFNAATSMLWGG 213
           K+  + + S  ALE    + + EV      L R     VN+G+   + + NA    + G 
Sbjct: 130 KISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGREMIGR 189

Query: 214 TVEGDQRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIF 273
            + G   D  A EFR  V++M  L G  N+ DF PSL   DLQG+  +M +L  +FD   
Sbjct: 190 RLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFL 249

Query: 274 ENMINQRLKIAGGEDGESAKNKDFLQVLLEVK--DAGDSKTPFTITHLKALLM 325
            +++ +        +G+  K+ D L  L+ +K  D        T T +KALL+
Sbjct: 250 SSILKEH-----EMNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLL 297

BLAST of Csor.00g308680 vs. NCBI nr
Match: KAG6579435.1 (Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 694 bits (1790), Expect = 6.51e-252
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120
           PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT
Sbjct: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120

Query: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180
           FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT
Sbjct: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180

Query: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240
           VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR
Sbjct: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240

Query: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300
           PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV
Sbjct: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300

Query: 301 LLEVKDAGDSKTPFTITHLKALLMGSSVQRTLVSIYCICFEIFQW 345
           LLEVKDAGDSKTPFTITHLKALLMGSSVQRTLVSIYCICFEIFQW
Sbjct: 301 LLEVKDAGDSKTPFTITHLKALLMGSSVQRTLVSIYCICFEIFQW 345

BLAST of Csor.00g308680 vs. NCBI nr
Match: XP_022970207.1 (geraniol 8-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 585 bits (1507), Expect = 7.04e-206
Identity = 291/331 (87.92%), Postives = 305/331 (92.15%), Query Frame = 0

Query: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           METTS+SLR LIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   METTSNSLRTLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120
           P GVPLLGNL FLDPELHTY R+LG+KYGPIVK+QLG KVGI+VNSPSVAREILKD+DVT
Sbjct: 61  PLGVPLLGNLLFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180
           FANRDVPQAGR A YG S+IVW PYGAEWRMLRKVCVLKMLSNA LESVY L RREVRNT
Sbjct: 121 FANRDVPQAGRAARYGGSNIVWNPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNT 180

Query: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240
           VA LY R+GSAVNVGEQSFLTIFN  TSMLWGGTVEGDQRDSLAAEFR+TVSD+T L G+
Sbjct: 181 VAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300
           PNVSDFFP LARFDLQGIEK+MHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQ 
Sbjct: 241 PNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQF 300

Query: 301 LLEVKDAGDSKTPFTITHLKALLMGSSVQRT 331
           LLEVKD+GDSKTP TITHLKALLM   +  T
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLMDMVIGGT 331

BLAST of Csor.00g308680 vs. NCBI nr
Match: KAG6579436.1 (Premnaspirodiene oxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 563 bits (1451), Expect = 2.84e-200
Identity = 280/324 (86.42%), Postives = 297/324 (91.67%), Query Frame = 0

Query: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           ME TS+SL+  IDSLNFL PW WID AD Q RFLFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   MEPTSNSLQTRIDSLNFLHPWRWIDAADNQSRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120
           P GVPLLGNLPFLDPELHTY R+LG+KYGPIVK+QLG KVGI+VNSPSVAREILKD+DVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180
           FANRDVPQAGR A+YG SDI WTPYG EWRMLRKVCV+KMLSNA LESVY L RREVRNT
Sbjct: 121 FANRDVPQAGRAATYGGSDIAWTPYGDEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180

Query: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240
           VA LY R+GSA+NVGEQSFLTIFN  TSMLWGGTV+GDQRDSLAAEFR+TVSD+T L G+
Sbjct: 181 VAILYGRAGSAMNVGEQSFLTIFNVVTSMLWGGTVKGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300
           PNVSDFFPSLARFDLQGIEK+MHKLAHKFDTIFENMINQRLKIAGG DGESAKNKDFLQ 
Sbjct: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGADGESAKNKDFLQF 300

Query: 301 LLEVKDAGDSKTPFTITHLKALLM 324
           LLEVKD+GDSKTP TITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308680 vs. NCBI nr
Match: XP_023551603.1 (geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570 bits (1469), Expect = 4.19e-200
Identity = 283/324 (87.35%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           METTS+SL+ LIDSLNFLLPW WI  AD Q R LFTVFFAGLLIFLYTKVTRRRV LPPG
Sbjct: 1   METTSNSLQTLIDSLNFLLPWRWIHAADNQSRLLFTVFFAGLLIFLYTKVTRRRVTLPPG 60

Query: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120
           P GVPLLGNLPFLDPELHTY R+LG+KYGPIVK+QLG KVGI+VNSPSVAREILKDHDVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDHDVT 120

Query: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180
           FANRDVPQAGR A+YG SDI WTPYGAEWRMLRKVCV+KMLSNA LESVY L RREVRNT
Sbjct: 121 FANRDVPQAGRAATYGGSDIAWTPYGAEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180

Query: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240
           VANLY R GSAVNVGEQSFLTIFN  TSMLWGG +EGDQRDSLAAEFR+TVSD+T L G+
Sbjct: 181 VANLYGRGGSAVNVGEQSFLTIFNVVTSMLWGGIMEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300
           PNVSDFFPSLARFDLQGIEK+MHKLAHKFDTIFENMINQRLKIAGGEDGESAK+KDFLQ 
Sbjct: 241 PNVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKSKDFLQF 300

Query: 301 LLEVKDAGDSKTPFTITHLKALLM 324
           LLEVKD+GDSKTP TITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308680 vs. NCBI nr
Match: XP_022970206.1 (geraniol 8-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 568 bits (1464), Expect = 2.41e-199
Identity = 283/324 (87.35%), Postives = 297/324 (91.67%), Query Frame = 0

Query: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           ME TS+ L+  IDSLNFLLPW WID AD Q R LFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   MEPTSNPLQIFIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120
           P GVPLLGNLPFLDPELHTY R+LG+KYGPIVK+QLG KVGI+VNSPSVAREILKD+DVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180
           FANRDVPQAGR ASYG SDI WTPYGAEWRMLRKVCVLKMLSNA LESVY L RREVRNT
Sbjct: 121 FANRDVPQAGRAASYGGSDIAWTPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNT 180

Query: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240
           VA LY R+GSAVNVGEQSFLTIFN  TSMLWGGTVEGDQRDSLAAEFR+TVSD+T L G+
Sbjct: 181 VAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300
           PNVSDFFP LARFDLQGIEK+MHKLAHKFDTIFENMINQRLKI+GGEDGESAKNKDFLQ 
Sbjct: 241 PNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKISGGEDGESAKNKDFLQF 300

Query: 301 LLEVKDAGDSKTPFTITHLKALLM 324
           LLEVKD+GDSKTP TITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308680 vs. ExPASy TrEMBL
Match: A0A6J1HYH5 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469169 PE=3 SV=1)

HSP 1 Score: 585 bits (1507), Expect = 3.41e-206
Identity = 291/331 (87.92%), Postives = 305/331 (92.15%), Query Frame = 0

Query: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           METTS+SLR LIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   METTSNSLRTLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120
           P GVPLLGNL FLDPELHTY R+LG+KYGPIVK+QLG KVGI+VNSPSVAREILKD+DVT
Sbjct: 61  PLGVPLLGNLLFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180
           FANRDVPQAGR A YG S+IVW PYGAEWRMLRKVCVLKMLSNA LESVY L RREVRNT
Sbjct: 121 FANRDVPQAGRAARYGGSNIVWNPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNT 180

Query: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240
           VA LY R+GSAVNVGEQSFLTIFN  TSMLWGGTVEGDQRDSLAAEFR+TVSD+T L G+
Sbjct: 181 VAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300
           PNVSDFFP LARFDLQGIEK+MHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQ 
Sbjct: 241 PNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQF 300

Query: 301 LLEVKDAGDSKTPFTITHLKALLMGSSVQRT 331
           LLEVKD+GDSKTP TITHLKALLM   +  T
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLMDMVIGGT 331

BLAST of Csor.00g308680 vs. ExPASy TrEMBL
Match: A0A6J1I001 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469168 PE=3 SV=1)

HSP 1 Score: 568 bits (1464), Expect = 1.17e-199
Identity = 283/324 (87.35%), Postives = 297/324 (91.67%), Query Frame = 0

Query: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           ME TS+ L+  IDSLNFLLPW WID AD Q R LFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   MEPTSNPLQIFIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120
           P GVPLLGNLPFLDPELHTY R+LG+KYGPIVK+QLG KVGI+VNSPSVAREILKD+DVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVT 120

Query: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180
           FANRDVPQAGR ASYG SDI WTPYGAEWRMLRKVCVLKMLSNA LESVY L RREVRNT
Sbjct: 121 FANRDVPQAGRAASYGGSDIAWTPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNT 180

Query: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240
           VA LY R+GSAVNVGEQSFLTIFN  TSMLWGGTVEGDQRDSLAAEFR+TVSD+T L G+
Sbjct: 181 VAILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300
           PNVSDFFP LARFDLQGIEK+MHKLAHKFDTIFENMINQRLKI+GGEDGESAKNKDFLQ 
Sbjct: 241 PNVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKISGGEDGESAKNKDFLQF 300

Query: 301 LLEVKDAGDSKTPFTITHLKALLM 324
           LLEVKD+GDSKTP TITHLKALLM
Sbjct: 301 LLEVKDSGDSKTPLTITHLKALLM 324

BLAST of Csor.00g308680 vs. ExPASy TrEMBL
Match: A0A6J1E5N3 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111430178 PE=3 SV=1)

HSP 1 Score: 554 bits (1428), Expect = 3.44e-194
Identity = 274/331 (82.78%), Postives = 295/331 (89.12%), Query Frame = 0

Query: 1   METTSDSLRPLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPG 60
           METTS+SL+ LIDSLNFLLPW WID AD Q R LFTVFFAGLLIFLYTKVTRRRVPLPPG
Sbjct: 1   METTSNSLQTLIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRRRVPLPPG 60

Query: 61  PPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVT 120
           P GVPLLGNLPFLDPELHTY R+LG+KYGPIVK+QLG KVGI+VNSPSVAREILKDHDVT
Sbjct: 61  PLGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDHDVT 120

Query: 121 FANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNT 180
           FANRDVPQAGR A+YG S+IVW PYG+EWRMLRKVCV+KMLSNA LESVY L RREVRNT
Sbjct: 121 FANRDVPQAGRAATYGGSNIVWNPYGSEWRMLRKVCVVKMLSNATLESVYDLRRREVRNT 180

Query: 181 VANLYRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGR 240
           VANLY R+GSAVNVGEQ FLT+FN  T MLWGG +EGDQRDSLAAEFR+TVSD T L G+
Sbjct: 181 VANLYGRAGSAVNVGEQGFLTVFNVVTGMLWGGIMEGDQRDSLAAEFRETVSDFTELLGK 240

Query: 241 PNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQV 300
           PNVSDFFPSLARFDLQGIEK+M KLA KFDTIFENMINQRLKI GGEDG+SA+N DFLQ 
Sbjct: 241 PNVSDFFPSLARFDLQGIEKKMRKLALKFDTIFENMINQRLKIVGGEDGQSAQNHDFLQF 300

Query: 301 LLEVKDAGDSKTPFTITHLKALLMGSSVQRT 331
           LLE+KDAGDSKTP T+ HLKALLM   +  T
Sbjct: 301 LLEMKDAGDSKTPLTMIHLKALLMDMVIGGT 331

BLAST of Csor.00g308680 vs. ExPASy TrEMBL
Match: A0A1S3ASY0 (flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482651 PE=3 SV=1)

HSP 1 Score: 462 bits (1188), Expect = 4.92e-158
Identity = 226/302 (74.83%), Postives = 258/302 (85.43%), Query Frame = 0

Query: 30  QFRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPPGVPLLGNLPFLDPELHTYLRELGRKYG 89
           Q  F FT F A LLIFLY ++TR RVPLPPGP GVPLLGNLPFLDPELHTY  +L +KYG
Sbjct: 13  QSHFFFT-FLAALLIFLYLRLTRLRVPLPPGPRGVPLLGNLPFLDPELHTYFAQLSQKYG 72

Query: 90  PIVKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEW 149
           PIVK+QLGRK+GI++NSPSV RE+LKDHDVTFANRDVP AGR ASYG SDIVWTPYG +W
Sbjct: 73  PIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPHAGRAASYGGSDIVWTPYGPQW 132

Query: 150 RMLRKVCVLKMLSNAALESVYKLLRREVRNTVANLYRRSGSAVNVGEQSFLTIFNAATSM 209
           RMLRKVCVLKMLSNA L+SVY+L RREVRNTVA+LY R+G+ VNVGEQ FLT+FN  TSM
Sbjct: 133 RMLRKVCVLKMLSNATLDSVYELRRREVRNTVAHLYARAGTRVNVGEQGFLTVFNVVTSM 192

Query: 210 LWGGTVEGDQRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHKF 269
           LWGG+VEG+QRD LAAEFR+TVS+MT L G PNVSDFFPSLARFD+QGIEK+M +LA +F
Sbjct: 193 LWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDIQGIEKKMRELAPRF 252

Query: 270 DTIFENMINQRLKIAGGEDGESAKNKDFLQVLLEVKDAGDSKTPFTITHLKALLMGSSVQ 329
           D+IFE MI+QRLKI G ++GES K  DFLQ LL+VKD G+SKTP T+THLKALLM   + 
Sbjct: 253 DSIFEKMIDQRLKIDGKDEGESVKKNDFLQFLLQVKDDGESKTPLTMTHLKALLMDMVIG 312

Query: 330 RT 331
            T
Sbjct: 313 GT 313

BLAST of Csor.00g308680 vs. ExPASy TrEMBL
Match: A0A0A0KR55 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159600 PE=3 SV=1)

HSP 1 Score: 461 bits (1186), Expect = 9.88e-158
Identity = 226/302 (74.83%), Postives = 259/302 (85.76%), Query Frame = 0

Query: 30  QFRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPPGVPLLGNLPFLDPELHTYLRELGRKYG 89
           Q  F FT F A LLIFLY ++TR RVPLPPGP GVPLLGNLPFL PELHTY  +LG+KYG
Sbjct: 13  QSHFFFT-FLAALLIFLYLRLTRLRVPLPPGPRGVPLLGNLPFLHPELHTYFAQLGQKYG 72

Query: 90  PIVKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEW 149
           PIVK+QLGRK+GI++NSPSV RE+LKDHDVTFANRDVPQAGR ASYG SDIVWTPYG +W
Sbjct: 73  PIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPQAGRAASYGGSDIVWTPYGPKW 132

Query: 150 RMLRKVCVLKMLSNAALESVYKLLRREVRNTVANLYRRSGSAVNVGEQSFLTIFNAATSM 209
           RMLRKVCV+KMLSNA L+SVY+L R+EVRNTVA+LY R+G+AVNVGEQ FLT+FN  TSM
Sbjct: 133 RMLRKVCVVKMLSNATLDSVYELRRKEVRNTVAHLYARAGTAVNVGEQGFLTVFNVVTSM 192

Query: 210 LWGGTVEGDQRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHKF 269
           LWGG+VEG+QRD LAAEFR+TVS+MT L G PNVSDFFPSLARFDLQGIEK+M +LA +F
Sbjct: 193 LWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDLQGIEKKMRELAPRF 252

Query: 270 DTIFENMINQRLKIAGGEDGESAKNKDFLQVLLEVKDAGDSKTPFTITHLKALLMGSSVQ 329
           D+IFE MI+QRLKI GG++G S K  DFLQ LL+VKD  +SKTP T+THLKALLM   + 
Sbjct: 253 DSIFEKMIDQRLKIDGGDEGGSVKKNDFLQFLLQVKDEEESKTPLTMTHLKALLMDMVIG 312

Query: 330 RT 331
            T
Sbjct: 313 GT 313

BLAST of Csor.00g308680 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 306.6 bits (784), Expect = 2.5e-83
Identity = 156/320 (48.75%), Postives = 214/320 (66.88%), Query Frame = 0

Query: 24  IDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPPGVPLLGNLPFLDPELHTYLRE 83
           I+   Y    L  +F   +L +L+ +  +    LPPGP G+P++GNLPFLDP+LHTY  +
Sbjct: 13  INLTPYAILILIAIF--SILWYLFKRSPQPH--LPPGPRGLPIVGNLPFLDPDLHTYFTK 72

Query: 84  LGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWT 143
           L   YGPI K+ LG K+ +VVN+PS+AREILKD D+ F+N DVP   R  +YG  D+VW 
Sbjct: 73  LAESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWL 132

Query: 144 PYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNTVANLYRR--SGSAVNVGEQSFLT 203
           PYGAEWRMLRKVCVLK+LS+  L S Y+L R+E+R     LY++    S VNVGEQ FLT
Sbjct: 133 PYGAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLT 192

Query: 204 IFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQ 263
           + N   +MLWGG+V+ ++ +S+  EF++ +S++T L G PNVSDFFP LARFDLQG+ K+
Sbjct: 193 MMNLTMNMLWGGSVKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKK 252

Query: 264 MHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQVLLEVKD-AGDSKTPFTITHLK 323
           MH  A + D I +  I Q +++    DG+  + KDFLQ L+++KD   DS+ P T+ H+K
Sbjct: 253 MHVCARELDAILDRAIEQ-MQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVK 312

Query: 324 ALLMGSSVQRTLVSIYCICF 341
           A+L+   V  T  S   I F
Sbjct: 313 AVLVDLVVGGTDTSTNTIEF 327

BLAST of Csor.00g308680 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 305.8 bits (782), Expect = 4.3e-83
Identity = 164/326 (50.31%), Postives = 213/326 (65.34%), Query Frame = 0

Query: 18  LLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPPGVPLLGNLPFLDPEL 77
           L P   I+   Y    L TVF   +L +++ +    +  LPPGP G+P++GNLPFLDP+L
Sbjct: 7   LFPDNTINLTPYAIVILTTVF--SILWYIFKR--SPQPSLPPGPRGLPIVGNLPFLDPDL 66

Query: 78  HTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLASYGS 137
           HTY   L + +GPI K+ LG K+ IVVNSPS+AREILKD D+ F+NRDVP  GR A+YG 
Sbjct: 67  HTYFANLAQSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGG 126

Query: 138 SDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNTVANLYR--RSGSAVNVG 197
            DIVWTPYGAEWR LRK+CVLK+LS   L+S Y+L R+EVR     LY   R  S V VG
Sbjct: 127 IDIVWTPYGAEWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVG 186

Query: 198 EQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDL 257
           +Q FLT+ N   +MLWGG+V+ ++ +S+  EF+  +S++T L   PNVSDFFP LARFDL
Sbjct: 187 DQLFLTMMNLTMNMLWGGSVKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDL 246

Query: 258 QGIEKQMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQVLLEVKD-AGDSKTPF 317
           QG+ K+M   A + D + +  I Q   + G +D E    KDFLQ L+++KD  GDS+ P 
Sbjct: 247 QGLVKRMGVCARELDAVLDRAIEQMKPLRGRDDDEV---KDFLQYLMKLKDQEGDSEVPI 306

Query: 318 TITHLKALLMGSSVQRTLVSIYCICF 341
           TI H+KALL    V  T  S   I F
Sbjct: 307 TINHVKALLTDMVVGGTDTSTNTIEF 325

BLAST of Csor.00g308680 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 297.0 bits (759), Expect = 2.0e-80
Identity = 158/331 (47.73%), Postives = 211/331 (63.75%), Query Frame = 0

Query: 15  LNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPGPPGVPLLGNLPFLD 74
           L+ L P   I    Y +  L       +L +++ +    + PLPPGP G+P++GNLPFLD
Sbjct: 4   LSNLFPDNAISLTPYAYAVLILTATFSILWYIFKR--SPQPPLPPGPRGLPIVGNLPFLD 63

Query: 75  PELHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSVAREILKDHDVTFANRDVPQAGRLAS 134
           P+LHTY  +L + +GPI K+ LG K+ +VVNSPS+A EILKD D+ F+N DVP   R  +
Sbjct: 64  PDLHTYFTKLAQSHGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVT 123

Query: 135 YGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESVYKLLRREVRNTVANLYRR--SGSAV 194
           YG  D+VW PYGAEWRMLRKVC  K+ S   L+S Y+L R+E+R     LY++    S V
Sbjct: 124 YGGLDLVWLPYGAEWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPV 183

Query: 195 NVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEFRKTVSDMTGLQGRPNVSDFFPSLAR 254
           NVGEQ FLT+ N   +MLWGG+V+ +  +S+  EF+  +S++T L G PNVSDFFP LAR
Sbjct: 184 NVGEQLFLTMMNLMMNMLWGGSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLAR 243

Query: 255 FDLQGIEKQMHKLAHKFDTIFENMIN--QRLKIAGGEDGESAKNKDFLQVLLEVKD-AGD 314
           FDLQG+ K+MH  A   D I +  I   QRL+   G+DGE    KDFLQ L++++D   D
Sbjct: 244 FDLQGLVKKMHLYARDLDAILDRAIEQMQRLRSRDGDDGEC---KDFLQHLMKLRDQEAD 303

Query: 315 SKTPFTITHLKALLMGSSVQRTLVSIYCICF 341
           S  P T+ H+KA+LM   V  T  S   I F
Sbjct: 304 SDVPITMNHVKAVLMDMVVGGTESSTNTIEF 329

BLAST of Csor.00g308680 vs. TAIR 10
Match: AT5G44620.1 (cytochrome P450, family 706, subfamily A, polypeptide 3 )

HSP 1 Score: 289.7 bits (740), Expect = 3.2e-78
Identity = 149/299 (49.83%), Postives = 201/299 (67.22%), Query Frame = 0

Query: 44  IFLYTKVTRRRVPLPPGPPGVPLLGNLPFLDPELHTYLRELGRKYGPIVKIQLGRKVGIV 103
           ++LY K  RR  PLPPGP G+P++GNLPFL PELHTY + L +K+GPI K+ LG K+ IV
Sbjct: 33  LWLYAKCKRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQGLAKKHGPIFKLWLGAKLTIV 92

Query: 104 VNSPSVAREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSN 163
           V S  VA+EILK +D+ FAN DVP  G + +YG ++I+W+PYG +WRMLRK+CV ++L N
Sbjct: 93  VTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGPKWRMLRKLCVNRILRN 152

Query: 164 AALESVYKLLRREVRNTVANL--YRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRD 223
           A L+S   L RRE R TV  L    R GS VN+GEQ FL + N  T MLWG TV+ ++R+
Sbjct: 153 AMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNVVTQMLWGTTVKEEERE 212

Query: 224 SLAAEFRKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRL 283
            + AEF + + +M  L   PN+SDFFP L+RFDLQG+ K+M + A + D +F+ +INQRL
Sbjct: 213 VVGAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKRMRRPAQRMDQMFDRIINQRL 272

Query: 284 KIAGGEDGESAKNKDFLQVLLEVKDAGDSKTPFTITHLKALLMGSSVQRTLVSIYCICF 341
            +    D    +  DFL VLL+VKD    KT  T+  +KA+LM   +  T  S++ I F
Sbjct: 273 GM--DRDSSDGRAVDFLDVLLKVKDEEAEKTKLTMNDVKAVLMDMVLGGTDTSLHVIEF 329

BLAST of Csor.00g308680 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 284.3 bits (726), Expect = 1.3e-76
Identity = 136/277 (49.10%), Postives = 192/277 (69.31%), Query Frame = 0

Query: 51  TRRRVPLPPGPPGVPLLGNLPFLDPE-LHTYLRELGRKYGPIVKIQLGRKVGIVVNSPSV 110
           +++  PLPPGP  +P++GNLPFL+ + LHT  + L  KYGP++KI LG K+ IVV+SP +
Sbjct: 47  SKKEPPLPPGPWPLPIVGNLPFLNSDVLHTQFQALTLKYGPLMKIHLGSKLAIVVSSPDM 106

Query: 111 AREILKDHDVTFANRDVPQAGRLASYGSSDIVWTPYGAEWRMLRKVCVLKMLSNAALESV 170
           ARE+LK HD+TFAN D+P+ G++ +YG  DI+W+PYG  WR LRK+CV+KM +  +LE+ 
Sbjct: 107 AREVLKTHDITFANHDLPEVGKINTYGGEDILWSPYGTHWRRLRKLCVMKMFTTPSLEAS 166

Query: 171 YKLLRREVRNTVANL--YRRSGSAVNVGEQSFLTIFNAATSMLWGGTVEGDQRDSLAAEF 230
           Y   R E R T+ ++    R GS VN+GEQ FL+IFN  T M+WG TVEGD+R SL  E 
Sbjct: 167 YSTRREETRQTIVHMSEMAREGSPVNLGEQIFLSIFNVVTRMMWGATVEGDERTSLGNEL 226

Query: 231 RKTVSDMTGLQGRPNVSDFFPSLARFDLQGIEKQMHKLAHKFDTIFENMINQRLKIAGGE 290
           +  +SD++ ++G  N SDFFP  +RFD QG+ KQM     K D +F+ ++   +K+ G  
Sbjct: 227 KTLISDISDIEGIQNYSDFFPLFSRFDFQGLVKQMKGHVKKLDLLFDRVMESHVKMVG-- 286

Query: 291 DGESAKNKDFLQVLLEVKDAGDSKTPFTITHLKALLM 325
             +S + +DFLQ LL VKD  D K P ++TH+K+LLM
Sbjct: 287 -KKSEEEEDFLQYLLRVKD-DDEKAPLSMTHVKSLLM 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A4D6Q4156.8e-4134.33Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Q7G6026.3e-3936.07Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
E9KBR88.3e-3928.79Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1[more]
Q9SBQ94.1e-3833.11Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1[more]
Q9SD855.4e-3833.79Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6579435.16.51e-252100.00Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022970207.17.04e-20687.92geraniol 8-hydroxylase-like [Cucurbita maxima][more]
KAG6579436.12.84e-20086.42Premnaspirodiene oxygenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023551603.14.19e-20087.35geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_022970206.12.41e-19987.35geraniol 8-hydroxylase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1HYH53.41e-20687.92geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469169 PE=3 SV=... [more]
A0A6J1I0011.17e-19987.35geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469168 PE=3 SV=... [more]
A0A6J1E5N33.44e-19482.78geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111430178 PE=3 S... [more]
A0A1S3ASY04.92e-15874.83flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482651 PE=3 SV=... [more]
A0A0A0KR559.88e-15874.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159600 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G12320.12.5e-8348.75cytochrome P450, family 706, subfamily A, polypeptide 6 [more]
AT4G12300.14.3e-8350.31cytochrome P450, family 706, subfamily A, polypeptide 4 [more]
AT4G12310.12.0e-8047.73cytochrome P450, family 706, subfamily A, polypeptide 5 [more]
AT5G44620.13.2e-7849.83cytochrome P450, family 706, subfamily A, polypeptide 3 [more]
AT4G22710.11.3e-7649.10cytochrome P450, family 706, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PFAMPF00067p450coord: 58..324
e-value: 4.1E-29
score: 101.6
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 44..340
e-value: 5.1E-46
score: 159.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 56..324
NoneNo IPR availablePANTHERPTHR47951:SF3CYTOCHROME P450, FAMILY 706, SUBFAMILY A, POLYPEPTIDE 3-RELATEDcoord: 33..330
NoneNo IPR availablePANTHERPTHR47951OS08G0547900 PROTEINcoord: 33..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g308680.m01Csor.00g308680.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen