Csor.00g308070 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g308070
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioncucumisin-like
LocationCsor_Chr15: 8754616 .. 8758727 (+)
RNA-Seq ExpressionCsor.00g308070
SyntenyCsor.00g308070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTCTTTCGAGGCTTCTCTTCCTCGCCTTTTGTTTATCTCAGCTATTCTTCAGCTCAAATTCCGAACACGATGATAGAAAAGTAACTCTAATTTTATCTCTAAATCTCCATTTACGTCGTATTTTTCGTTTAATATGTCGTTTTTTGATTTGCAGACGTATATTGTGTACATGGGAAGTCATCCAAAAGAACGAGTTTTGACTCGGTCTCATCATGTGAGAATGCTACAAGAAACTATCGGCAGGTGATTGTTTAGGTTAAGTTACGAATCCAGTCTGTGAACGTTTATGTTTGTGTCTAACAAATCTTTATAGTTTGATAAGTATGGAATAAGTCGTCCTTGAACTTTAAATTGTGTCAAATAGTTTTTACTCGAATTTATTAGACGTAAATTTTTTAAAATTTAAAGATTTATGGTCGAGAAATATACTAATTAACCAAAATAAAACTTTATTTCAGTAGTTTTGCTCCACATTCTTTACTCCATAGCTACGAGAGAAGTTTCAATGGCTTTGTTGCGAAACTGACTGAAGTCGAAGTCCAAAAAGTTTCAGGTACGCTCGTAAAGAACCTAAGATTAGGTTCTTTTTCTCTGCGAAGATCGAGAGAGTGAAAACATTTCCAGCGAGTTAAGAACATAATGTAAAATGTTTGAACAGTATGGTTGAATATTCTCGGGTCTTGGAAACATCCCTAAAATATACCATTTTAATTCTCTCCTAAAAAAAATAATAATAATATATATATTTTTAATTTTAGAAATGAAGGGCGTAATATCGGTTTTTCTAAATGGAAAAAAACAACTCCACACAACAAGATCATGGGACTTCATGGGACTGTCCCAACAAGCTAGCCGAGTTCCATCAGTGGAAAGTGACATCATTGTCGGAGTACTAGATACAGGGATTTGGCCGGAATCGCCTAGTTTCCTCGACCAAGGATATGGCCCGCCACCACCTAAGTGGAAGGGTAGTTGTGAAGTCTCATCCGATTTCTCTTGTAACAAGTACGTTCAATTTCGTATATTCTCACAGATTTTTGCATAGTAATTTTAGGATTCGACATTTAATTTGTAAGAACATAAGTCATAATATTAGATCCTCTATGTAGTAAAATCATTGGAGCTCGATCATATCGTACCAATGGTCAATATCCCATAAACGATATCCAAGGTCCAAGAGATTCAAATGGCCATGGGACGCATGCGGCATCAACAGTAGCAGGCGAGTTGGTCAGACAAGCGAGTATGCTTGGTCTTGGCTCTGGCACAGCAAGGGGAGGAGTCCCATCCGCACGAATCGCTTCCTACAAAATATGTTGGTCTGACGGCTGCCGTGACGCTGATGTTCTTGCCGCATTCGACGATGCCATTGCCGATGGGGTTGATATCATCTCTTTCTCAGTCGGAGGACGCAAGCCAAGAGATTACTTTAATGACTCTATAGCCATTGGAGCATTCCATGCAATGAAAAAAGGAATCCTTACGTCAATGTCGGCTGGCAACAGCGGTCCAAAATCTTTCACTGTTAGAAACTTCTCACCCTGGTCTTTATCAGTAGCAGCTAGTACTACTGATAGGAAACTTTTAACCGGAGTTCAACTTGGAGATGGAAGAAGCTTCGATGTAAGTTCATCCATTACTAATTCGATTTTATCATACATTAAAGTTAAGAAGGCAATTTGTTAAATATATATACATTTCATTTGGATGCAGGGTGTCACAGTTAATACATTTGATTTAAATGGAACACAATATCCATTGGTGTATGCCGGAAATATTCCTAATGTTGGCTTCAATGGATCTATCTCCCGGTACGGTTTAAAACCTAAGCTTATAAATACTATTTTCATCATTAAATTTTCTTGTTCCTATTTTTTTAACCATGAAGATAAACTGAGTTAGATCCGAGTTGAGTTTCGTTGCTATTTTTTTTAATTATAAACGGAAAATTAAGATTTTTTCTTTTGTGAAATAGATTTTGTCTGACGAACTCGGTGGATAAGGAGTCGGTGAAGGGTAAAATTGTCCTTTGTGATTTTTTTATATCACCGACAAATTTGAGCTTTTTAGAAGGTGCAATCGGGATTATAATGCAAGACAACAATCCAAAGGATCTGACATTCCCTTTCCCTTTGCCTGCCTCCCATCTCGGCACACAAGAAGGAGCTCTCATTTCTTCTTATGCCAATTTAACTAGGTAATTAATCCAATCTTTTTTTACTTTTAATCTCAGTAATTACCATTACTTAACACTAATTAATTTCATCATTATTTACAGCCTTCCAACGGCTACTATTTTGAAAAGCACTGAAGGGAAGTATAAAGCAACCCCTTTTGTTGCATCCTTCTCTTCAAGAGGTCCAAATCCAATAACCCCTGATATTCTGAAGGTGATTAGCTTCATAATCATAAACTTAATCACTCTCTAATCTTGATTATTATTAATATTATTGAGTTGTTTATTGTGGCATTGCAGCCAGATTTGAGTGGTCCAGGTGTTGAAATTCTAGCCGCATGGAGCCCTATAGGCCCACCCTCAGGAGCTGAGGAAGATACCAGACAACTCCTTTTTAATATCATTTCAGGGACTTCAATGTCTTGCCCACATGCTACCGCTGTTGCTGCTTATGTTAAGTCGTTTCACCCTTCTTGGTCTCCCGCTGCCCTCAAATCGGCACTTATGACGACGGGTACTAACACCAATACTTGACTCGTAGCTAGCTTGCATAGAGATGTCTACCGGGTGAGATGGGAAAGCTTTTCATGTTCCCGTATTCACCTCTGTAAAATTTGTGGTAATTTAGTTCCCAGTGGATAGTCTTCCTCTTTGGACTTTCCCTTATAAAGAGTGTTTCGTGTTTCGTTTTTCTCCCCAACCAATGTGGGATATCACAATTCACTCCTGTTCCAAGCCCAACATCCTTGCTGGTATTCATTCTTTCTCCAATTGATGTGGGACCCCGCCAAATCTACCCTCTTTTGGGGCTAGCGTCCTTACTGACACACTCCCTCGTGTCTACCGCCTTCGAGGAAGAACCTCCTCGCTGGCACATAGTCCGGTGTCTAACTTTGATACCATTTGTAATGGCCTAAGCCCATTACAAATATTGTCTTCTTTGGGCTTTCTCTCGAAACATTAAAAAGCATCTTTCAGAGTTACAAAACCGGTATTCATCTAGATATTGTCCTCTTTGGACTTTCCCTTCTGAGTTTCCCCTCAAGGTTTTTGGGTTTTTCAGCTTCCCCCATTACATTACAGAAAGGTTTCCATACACTTATAAAGTGTGTTTAGTTCTCCTTCCCAACTAACGTATTTCAATATATATAATGAGGCAGGACGGGGATTTATGCAGGGTTTGGAGCAAGTTCGGGGATGGAGAATATAATCACTATACTCGGTCTCCTCACTCTCTCTCCCATTTAAATAGAGATTCTCCTCCTCGTTCGAGATCGGTCCTACAGGACAAATGGATATTTCTAAGCGTGCACTCATTTTAACGATGTTTATAGATTATTAACGCGATTAATTTACGCCATAGATTACTAACCAAATGAATTTACGAATGCAGCATTTCCCATGAGATCTGACCTTAACCCAGATGCAGAATTTGCATATGGCTCAGGCCATATAAACCCTTTAGGCGCAGTGAATCCCGGACTGATCTACAATGCCACTGAGATCGACTATGTAAGGTTCCTTTGTGGTCAAGGTTACAGCACCAAGTTGATCCAGCAAGTTTCTGGAGATAACAGCTCGTGCTCTCGAAGCGATTCCGACGTAGTTTTCGATCTAAACTATCCTTCGTTCGCTCTTTCCACGCCTATCTCAACCTCGATCAGCCAAGTTTATAGAAGAAAGGTCACAAATGTTGGGTCGGCGAATTCGATATATAAGGCCACAGTTTCGGGTCCTTCGGGGCTTAAAATTACAGTAAATCCTTCTGTTCTTTCGTTCAAGGCCTTGGGAGAGGAGCTGAGCTTTGAGGTTACAATTGAAGGAAGCATCAGCAGTAGCATTGCGTCGGCTTCATTGGTGTGGGATGATGGCCAACACAAAGTGAGGAGTCCTATAATAGTCTTTGATTCTAATAAGTTTATTAATTAA

mRNA sequence

ATGTCTTCTCTTTCGAGGCTTCTCTTCCTCGCCTTTTGTTTATCTCAGCTATTCTTCAGCTCAAATTCCGAACACGATGATAGAAAAACGTATATTGTGTACATGGGAAGTCATCCAAAAGAACGAGTTTTGACTCGGTCTCATCATGTGAGAATGCTACAAGAAACTATCGGCAGTAGTTTTGCTCCACATTCTTTACTCCATAGCTACGAGAGAAGTTTCAATGGCTTTGTTGCGAAACTGACTGAAGTCGAAGTCCAAAAAGTTTCAGAAATGAAGGGCGTAATATCGGTTTTTCTAAATGGAAAAAAACAACTCCACACAACAAGATCATGGGACTTCATGGGACTGTCCCAACAAGCTAGCCGAGTTCCATCAGTGGAAAGTGACATCATTGTCGGAGTACTAGATACAGGGATTTGGCCGGAATCGCCTAGTTTCCTCGACCAAGGATATGGCCCGCCACCACCTAAGTGGAAGGGTAGTTGTGAAGTCTCATCCGATTTCTCTTGTAACAATAAAATCATTGGAGCTCGATCATATCGTACCAATGGTCAATATCCCATAAACGATATCCAAGGTCCAAGAGATTCAAATGGCCATGGGACGCATGCGGCATCAACAGTAGCAGGCGAGTTGGTCAGACAAGCGAGTATGCTTGGTCTTGGCTCTGGCACAGCAAGGGGAGGAGTCCCATCCGCACGAATCGCTTCCTACAAAATATGTTGGTCTGACGGCTGCCGTGACGCTGATGTTCTTGCCGCATTCGACGATGCCATTGCCGATGGGGTTGATATCATCTCTTTCTCAGTCGGAGGACGCAAGCCAAGAGATTACTTTAATGACTCTATAGCCATTGGAGCATTCCATGCAATGAAAAAAGGAATCCTTACGTCAATGTCGGCTGGCAACAGCGGTCCAAAATCTTTCACTGTTAGAAACTTCTCACCCTGGTCTTTATCAGTAGCAGCTAGTACTACTGATAGGAAACTTTTAACCGGAGTTCAACTTGGAGATGGAAGAAGCTTCGATGGTGTCACAGTTAATACATTTGATTTAAATGGAACACAATATCCATTGGTGTATGCCGGAAATATTCCTAATGTTGGCTTCAATGGATCTATCTCCCGATTTTGTCTGACGAACTCGGTGGATAAGGAGTCGGTGAAGGGTAAAATTGTCCTTTGTGATTTTTTTATATCACCGACAAATTTGAGCTTTTTAGAAGGTGCAATCGGGATTATAATGCAAGACAACAATCCAAAGGATCTGACATTCCCTTTCCCTTTGCCTGCCTCCCATCTCGGCACACAAGAAGGAGCTCTCATTTCTTCTTATGCCAATTTAACTAGCCTTCCAACGGCTACTATTTTGAAAAGCACTGAAGGGAAGTATAAAGCAACCCCTTTTGTTGCATCCTTCTCTTCAAGAGGTCCAAATCCAATAACCCCTGATATTCTGAAGCCAGATTTGAGTGGTCCAGGTGTTGAAATTCTAGCCGCATGGAGCCCTATAGGCCCACCCTCAGGAGCTGAGGAAGATACCAGACAACTCCTTTTTAATATCATTTCAGGGACTTCAATGTCTTGCCCACATGCTACCGCTGTTGCTGCTTATGTTAAGTCGTTTCACCCTTCTTGGTCTCCCGCTGCCCTCAAATCGGCACTTATGACGACGGCATTTCCCATGAGATCTGACCTTAACCCAGATGCAGAATTTGCATATGGCTCAGGCCATATAAACCCTTTAGGCGCAGTGAATCCCGGACTGATCTACAATGCCACTGAGATCGACTATGTAAGGTTCCTTTGTGGTCAAGGTTACAGCACCAAGTTGATCCAGCAAGTTTCTGGAGATAACAGCTCGTGCTCTCGAAGCGATTCCGACGTAGTTTTCGATCTAAACTATCCTTCGTTCGCTCTTTCCACGCCTATCTCAACCTCGATCAGCCAAGTTTATAGAAGAAAGGTCACAAATGTTGGGTCGGCGAATTCGATATATAAGGCCACAGTTTCGGGTCCTTCGGGGCTTAAAATTACAGTAAATCCTTCTGTTCTTTCGTTCAAGGCCTTGGGAGAGGAGCTGAGCTTTGAGGTTACAATTGAAGGAAGCATCAGCAGTAGCATTGCGTCGGCTTCATTGGTGTGGGATGATGGCCAACACAAAGTGAGGAGTCCTATAATAGTCTTTGATTCTAATAAGTTTATTAATTAA

Coding sequence (CDS)

ATGTCTTCTCTTTCGAGGCTTCTCTTCCTCGCCTTTTGTTTATCTCAGCTATTCTTCAGCTCAAATTCCGAACACGATGATAGAAAAACGTATATTGTGTACATGGGAAGTCATCCAAAAGAACGAGTTTTGACTCGGTCTCATCATGTGAGAATGCTACAAGAAACTATCGGCAGTAGTTTTGCTCCACATTCTTTACTCCATAGCTACGAGAGAAGTTTCAATGGCTTTGTTGCGAAACTGACTGAAGTCGAAGTCCAAAAAGTTTCAGAAATGAAGGGCGTAATATCGGTTTTTCTAAATGGAAAAAAACAACTCCACACAACAAGATCATGGGACTTCATGGGACTGTCCCAACAAGCTAGCCGAGTTCCATCAGTGGAAAGTGACATCATTGTCGGAGTACTAGATACAGGGATTTGGCCGGAATCGCCTAGTTTCCTCGACCAAGGATATGGCCCGCCACCACCTAAGTGGAAGGGTAGTTGTGAAGTCTCATCCGATTTCTCTTGTAACAATAAAATCATTGGAGCTCGATCATATCGTACCAATGGTCAATATCCCATAAACGATATCCAAGGTCCAAGAGATTCAAATGGCCATGGGACGCATGCGGCATCAACAGTAGCAGGCGAGTTGGTCAGACAAGCGAGTATGCTTGGTCTTGGCTCTGGCACAGCAAGGGGAGGAGTCCCATCCGCACGAATCGCTTCCTACAAAATATGTTGGTCTGACGGCTGCCGTGACGCTGATGTTCTTGCCGCATTCGACGATGCCATTGCCGATGGGGTTGATATCATCTCTTTCTCAGTCGGAGGACGCAAGCCAAGAGATTACTTTAATGACTCTATAGCCATTGGAGCATTCCATGCAATGAAAAAAGGAATCCTTACGTCAATGTCGGCTGGCAACAGCGGTCCAAAATCTTTCACTGTTAGAAACTTCTCACCCTGGTCTTTATCAGTAGCAGCTAGTACTACTGATAGGAAACTTTTAACCGGAGTTCAACTTGGAGATGGAAGAAGCTTCGATGGTGTCACAGTTAATACATTTGATTTAAATGGAACACAATATCCATTGGTGTATGCCGGAAATATTCCTAATGTTGGCTTCAATGGATCTATCTCCCGATTTTGTCTGACGAACTCGGTGGATAAGGAGTCGGTGAAGGGTAAAATTGTCCTTTGTGATTTTTTTATATCACCGACAAATTTGAGCTTTTTAGAAGGTGCAATCGGGATTATAATGCAAGACAACAATCCAAAGGATCTGACATTCCCTTTCCCTTTGCCTGCCTCCCATCTCGGCACACAAGAAGGAGCTCTCATTTCTTCTTATGCCAATTTAACTAGCCTTCCAACGGCTACTATTTTGAAAAGCACTGAAGGGAAGTATAAAGCAACCCCTTTTGTTGCATCCTTCTCTTCAAGAGGTCCAAATCCAATAACCCCTGATATTCTGAAGCCAGATTTGAGTGGTCCAGGTGTTGAAATTCTAGCCGCATGGAGCCCTATAGGCCCACCCTCAGGAGCTGAGGAAGATACCAGACAACTCCTTTTTAATATCATTTCAGGGACTTCAATGTCTTGCCCACATGCTACCGCTGTTGCTGCTTATGTTAAGTCGTTTCACCCTTCTTGGTCTCCCGCTGCCCTCAAATCGGCACTTATGACGACGGCATTTCCCATGAGATCTGACCTTAACCCAGATGCAGAATTTGCATATGGCTCAGGCCATATAAACCCTTTAGGCGCAGTGAATCCCGGACTGATCTACAATGCCACTGAGATCGACTATGTAAGGTTCCTTTGTGGTCAAGGTTACAGCACCAAGTTGATCCAGCAAGTTTCTGGAGATAACAGCTCGTGCTCTCGAAGCGATTCCGACGTAGTTTTCGATCTAAACTATCCTTCGTTCGCTCTTTCCACGCCTATCTCAACCTCGATCAGCCAAGTTTATAGAAGAAAGGTCACAAATGTTGGGTCGGCGAATTCGATATATAAGGCCACAGTTTCGGGTCCTTCGGGGCTTAAAATTACAGTAAATCCTTCTGTTCTTTCGTTCAAGGCCTTGGGAGAGGAGCTGAGCTTTGAGGTTACAATTGAAGGAAGCATCAGCAGTAGCATTGCGTCGGCTTCATTGGTGTGGGATGATGGCCAACACAAAGTGAGGAGTCCTATAATAGTCTTTGATTCTAATAAGTTTATTAATTAA

Protein sequence

MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVTNVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQHKVRSPIIVFDSNKFIN
Homology
BLAST of Csor.00g308070 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 2.7e-236
Identity = 414/732 (56.56%), Postives = 520/732 (71.04%), Query Frame = 0

Query: 5   SRLLFLAFCLSQLFFSS------NSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIG 64
           S L+F  F  S LFFS+      +S+ D +  YIVYMG   ++      HH  ML++ +G
Sbjct: 3   SSLIFKLFFFS-LFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVG 62

Query: 65  SSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLS 124
           S+FAP S+LH+Y+RSFNGF  KLTE E +K++ M+GV+SVFLN   +LHTTRSWDF+G  
Sbjct: 63  STFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP 122

Query: 125 QQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGA 184
               R   VES+I+VGVLDTGIWPESPSF D+G+ PPPPKWKG+CE S++F CN KIIGA
Sbjct: 123 LTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGA 182

Query: 185 RSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIAS 244
           RSY         D+ GPRD+NGHGTH AST AG LV QA++ GLG GTARGGVP ARIA+
Sbjct: 183 RSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAA 242

Query: 245 YKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILT 304
           YK+CW+DGC D D+LAA+DDAIADGVDIIS SVGG  PR YF D+IAIG+FHA+++GILT
Sbjct: 243 YKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILT 302

Query: 305 SMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQY 364
           S SAGN GP  FT  + SPW LSVAAST DRK +T VQ+G+G+SF GV++NTFD     Y
Sbjct: 303 SNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYY 362

Query: 365 PLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTN-LSFLEGAIGIIMQ 424
           PLV   +IPN GF+ S SRFC   SV+   +KGKIV+C+    P      L+GA G++M 
Sbjct: 363 PLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM- 422

Query: 425 DNNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSR 484
            +N +D    +PLP+S L   +      Y      P ATI KST     + P V SFSSR
Sbjct: 423 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 482

Query: 485 GPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVA 544
           GPN  T D++KPD+SGPGVEILAAW  + P  G   +T   LFNIISGTSMSCPH T +A
Sbjct: 483 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNT---LFNIISGTSMSCPHITGIA 542

Query: 545 AYVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEI 604
            YVK+++P+WSPAA+KSALMTTA PM +  NP AEFAYGSGH+NPL AV PGL+Y+A E 
Sbjct: 543 TYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANES 602

Query: 605 DYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKV 664
           DYV+FLCGQGY+T+ +++++GD S+C+  ++  V+DLNYPSF LS   S + +Q + R +
Sbjct: 603 DYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTL 662

Query: 665 TNVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWD 724
           T+V    S Y+A +S P GL I+VNP+VLSF  LG+  SF +T+ GSI   + SASLVW 
Sbjct: 663 TSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWS 722

Query: 725 DGQHKVRSPIIV 730
           DG H VRSPI +
Sbjct: 723 DGVHYVRSPITI 727

BLAST of Csor.00g308070 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 6.9e-192
Identity = 361/738 (48.92%), Postives = 483/738 (65.45%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRK---TYIVYMGSHPKERVLTRSHHVRMLQETI 60
           M+ LS  L+L  CL+ +F    S +D R+    YIVYMG+ P+ +    SHH+ +LQ+ +
Sbjct: 1   MAKLSTPLYL-ICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLV 60

Query: 61  GSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGL 120
           G+  A H L+ SY+RSFNGF A L++ E QK+  MK V+SVF +   +L TTRSWDF+G 
Sbjct: 61  GTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGF 120

Query: 121 SQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIG 180
            ++A R    ESD+IVGV+D+GIWPES SF D+G+GPPP KWKGSC+    F+CNNK+IG
Sbjct: 121 GEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIG 180

Query: 181 ARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIA 240
           AR Y              RD  GHGTH AST AG  V+ AS  GL  GTARGGVPSARIA
Sbjct: 181 ARFYN-------KFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIA 240

Query: 241 SYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGIL 300
           +YK+C+ + C D D+LAAFDDAIADGVD+IS S+      +  N S+AIG+FHAM +GI+
Sbjct: 241 AYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGII 300

Query: 301 TSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQ 360
           T+ SAGN+GP   +V N SPW ++VAAS TDR+ +  V LG+G++  G++VNTF+LNGT+
Sbjct: 301 TAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTK 360

Query: 361 YPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQ 420
           +P+VY  N+     + + + +C +  VD E VKGKIVLCD F+     ++L GAIG+I+Q
Sbjct: 361 FPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQ 420

Query: 421 DNNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSR 480
           +    D  F  P PAS LG ++   I SY      P A IL++ E   +  P+V SFSSR
Sbjct: 421 NTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSR 480

Query: 481 GPNPITPDILKPDLSGPGVEILAAWSPIGPPSG--AEEDTRQLLFNIISGTSMSCPHATA 540
           GP+ +  ++LKPD+S PG+EILAA+SP+  PS     ED R + ++++SGTSM+CPH   
Sbjct: 481 GPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAG 540

Query: 541 VAAYVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNAT 600
           VAAYVKSFHP WSP+A+KSA+MTTA PM    NP+ EFAYGSG INP  A +PGL+Y   
Sbjct: 541 VAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVE 600

Query: 601 EIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRR 660
             DY++ LC +G+ +  +   SG N +C  S+   V DLNYP+           +  ++R
Sbjct: 601 TEDYLKMLCAEGFDSTTLTTTSGQNVTC--SERTEVKDLNYPTMTTFVSSLDPFNVTFKR 660

Query: 661 KVTNVGSANSIYKATVSG-PSGLKITVNPSVLSFKALGEELSFEVTIEGS--ISSSIASA 720
            VTNVG  NS YKA+V      L+I++ P +L F  L E+ SF VTI G      S  S+
Sbjct: 661 TVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSS 720

Query: 721 SLVWDDGQHKVRSPIIVF 731
           S+VW DG H VRSPI+ +
Sbjct: 721 SVVWSDGSHSVRSPIVAY 725

BLAST of Csor.00g308070 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 8.4e-190
Identity = 358/746 (47.99%), Postives = 495/746 (66.35%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSS-NSEHDDRKTYIVYMGSHPKERVLT-RSHHVRMLQETIG 60
           M++L+    L  CL  LF SS ++  DD++ YIVYMGS       T  S H+ +LQE  G
Sbjct: 1   MATLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 60

Query: 61  SSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLS 120
            S     L+ SY+RSFNGF A+LTE E ++V++M GV+SVF N K QL TT SWDFMGL 
Sbjct: 61  ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 120

Query: 121 Q--QASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKII 180
           +  +  R P+VESD I+GV+D+GI PES SF D+G+GPPP KWKG C    +F+CNNK+I
Sbjct: 121 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 180

Query: 181 GARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARI 240
           GAR Y +         +G RD +GHGTH AST AG  V  AS  G+G+GT RGGVP++R+
Sbjct: 181 GARDYTS---------EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRV 240

Query: 241 ASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGI 300
           A+YK+C   GC    +L+AFDDAIADGVD+I+ S+G +    + ND IAIGAFHAM KG+
Sbjct: 241 AAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGV 300

Query: 301 LTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGT 360
           LT  SAGNSGPK  +V   +PW L+VAASTT+R  +T V LG+G++  G +VN +++ G 
Sbjct: 301 LTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGK 360

Query: 361 QYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLE--GAIGI 420
            YPLVY  +  +   +   +  C  + VDK  VKGKI++C     P  L  +E  GA+G+
Sbjct: 361 DYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG---GPGGLKIVESVGAVGL 420

Query: 421 IMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASF 480
           I +   P D+ F  PLPA+ L T++   + SY   T  P A +LK+     + +P +ASF
Sbjct: 421 IYRTPKP-DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASF 480

Query: 481 SSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHAT 540
           SSRGPN I  DILKPD++ PGVEILAA+SP G PS  ++DTR + ++++SGTSMSCPH  
Sbjct: 481 SSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVA 540

Query: 541 AVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLN--PDAEFAYGSGHINPLGAVNPGLIY 600
            VAAYVK+F+P WSP+ ++SA+MTTA+P+ +        EFAYGSGH++P+ A NPGL+Y
Sbjct: 541 GVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVY 600

Query: 601 NATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPIS-TSISQ 660
              + D++ FLCG  Y++++++ +SG+  +CS +   +  +LNYPS +     S T+ + 
Sbjct: 601 ELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTV 660

Query: 661 VYRRKVTNVGSANSIYKATVSGPSG--LKITVNPSVLSFKALGEELSFEVTIEGS-ISSS 720
            + R +TNVG+ NS Y + V    G  L + + PSVLSFK + E+ SF VT+ GS + S 
Sbjct: 661 TFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSE 720

Query: 721 I-ASASLVWDDGQHKVRSPIIVFDSN 734
           + +SA+L+W DG H VRSPI+V+ S+
Sbjct: 721 VPSSANLIWSDGTHNVRSPIVVYTSD 731

BLAST of Csor.00g308070 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.1e-184
Identity = 348/743 (46.84%), Postives = 485/743 (65.28%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSE--HDDRKTYIVYMGS-HPKERVLTRSHHVRMLQETI 60
           M++L+    L   L  L  SS S    +D + YIVYMGS   +   +  S H+ +LQ+  
Sbjct: 1   MANLAASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVT 60

Query: 61  GSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGL 120
           G S     L+ SY+RSFNGF A+LTE E   ++E++GV+SVF N   QLHTT SWDFMG+
Sbjct: 61  GESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGV 120

Query: 121 SQ--QASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKI 180
            +     R  ++ESD I+GV+DTGIWPES SF D+G+GPPP KWKG C    +F+CNNK+
Sbjct: 121 KEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKL 180

Query: 181 IGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSAR 240
           IGAR Y +         +G RD++GHGTH AST AG  V+  S  G+G+GT RGGVP++R
Sbjct: 181 IGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASR 240

Query: 241 IASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKG 300
           IA+YK+C   GC    +L++FDDAIADGVD+I+ S+G + P  + +D IAIGAFHAM KG
Sbjct: 241 IAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKG 300

Query: 301 ILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNG 360
           ILT  SAGNSGPK  TV + +PW  +VAASTT+R  +T V LG+G++  G +VN FD+ G
Sbjct: 301 ILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKG 360

Query: 361 TQYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGII 420
            +YPLVY  +  +   +   +  C    ++K  VKGKI++C     P+     +    I 
Sbjct: 361 KKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIA 420

Query: 421 MQDNNPK-DLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASF 480
           + D +P+ D+ F   LPAS L  ++   + SY      P A +LK+     + +P +ASF
Sbjct: 421 IIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASF 480

Query: 481 SSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHAT 540
           SSRGPN I  DILKPD++ PGVEILAA+SP G PS  E+DTR++ +++ SGTSM+CPH  
Sbjct: 481 SSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVA 540

Query: 541 AVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLN--PDAEFAYGSGHINPLGAVNPGLIY 600
            VAAYVK+F+P WSP+ ++SA+MTTA+P+++        EFAYG+GH++P+ A+NPGL+Y
Sbjct: 541 GVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVY 600

Query: 601 NATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSF-ALSTPISTSISQ 660
              + D++ FLCG  Y++K ++ +SGD   CS+ +  +  +LNYPS  A  +   ++ S 
Sbjct: 601 ELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSV 660

Query: 661 VYRRKVTNVGSANSIYKATVSGPSG--LKITVNPSVLSFKALGEELSFEVTIEGS-ISSS 720
            + R +TNVG+ NS YK+ V    G  L I V PSVL FK + E+ SF VT+ GS + S 
Sbjct: 661 TFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSE 720

Query: 721 I-ASASLVWDDGQHKVRSPIIVF 731
           + +SA+L+W DG H VRSPI+V+
Sbjct: 721 VPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Csor.00g308070 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 2.4e-181
Identity = 351/744 (47.18%), Postives = 480/744 (64.52%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNS----EHDDRKTYIVYMGSHP-KERVLTRSHHVRMLQE 60
           M+  +  +FL   L  L  SS S    +H D++ YIVY+GS P +E     S H+ +LQE
Sbjct: 1   MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQE 60

Query: 61  TIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFM 120
             G S   + L+ SY++SFNGF A+LTE E ++++ M+ V+SVF + K +L TT SW+FM
Sbjct: 61  ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120

Query: 121 GLSQ--QASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNN 180
           GL +  +  R  S+ESD I+GV+D+GI+PES SF DQG+GPPP KWKG+C    +F+CNN
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180

Query: 181 KIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPS 240
           K+IGAR Y    +      Q  RD +GHGTH AS  AG  V  ++  GLG+GTARGGVP+
Sbjct: 181 KVIGARDYTAKSKAN----QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPA 240

Query: 241 ARIASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMK 300
           ARIA YK+C ++GC    +++AFDDAIADGVD+IS S+       +  D IAIGAFHAM 
Sbjct: 241 ARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMA 300

Query: 301 KGILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDL 360
            G+LT  +AGN+GPK  TV + +PW  SVAAS T+R  +  V LGDG+   G +VNT+D+
Sbjct: 301 VGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDM 360

Query: 361 NGTQYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIG 420
           NGT YPLVY  +      +   +R C    +D + VKGKIVLCD          L GA+G
Sbjct: 361 NGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL-GAVG 420

Query: 421 IIMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVAS 480
            I+++  P D  F    P S L   +   + SY N T  P AT+LKS E   +  P VAS
Sbjct: 421 SIVKNPEP-DRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVAS 480

Query: 481 FSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHA 540
           FSSRGP+ I  DILKPD++ PGVEILAA+SP   P+ +E DTR++ ++++SGTSM+CPH 
Sbjct: 481 FSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHV 540

Query: 541 TAVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLN--PDAEFAYGSGHINPLGAVNPGLI 600
             VAAYVK+FHP WSP+ ++SA+MTTA+PM +  +     EFAYGSGH++P+ A+NPGL+
Sbjct: 541 AGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLV 600

Query: 601 YNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVV-FDLNYPSFALSTPISTSIS 660
           Y  T+ D++ FLCG  Y++  ++ +SGDNS+C++  S  +  +LNYP+ +     +   +
Sbjct: 601 YELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFN 660

Query: 661 QVYRRKVTNVGSANSIYKATVSGPSG--LKITVNPSVLSFKALGEELSFEVTIEGSI--S 720
             ++R VTNVG   S Y A V    G  L I V+P VLS K++ E+ SF VT+      +
Sbjct: 661 ITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGT 720

Query: 721 SSIASASLVWDDGQHKVRSPIIVF 731
               SA+L+W DG H VRSPIIV+
Sbjct: 721 KQPVSANLIWSDGTHNVRSPIIVY 738

BLAST of Csor.00g308070 vs. NCBI nr
Match: KAG6579374.1 (hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1462 bits (3786), Expect = 0.0
Identity = 737/737 (100.00%), Postives = 737/737 (100.00%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS
Sbjct: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
           ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS
Sbjct: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM
Sbjct: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL
Sbjct: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360

Query: 361 VYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNN 420
           VYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNN
Sbjct: 361 VYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNN 420

Query: 421 PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN 480
           PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN
Sbjct: 421 PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN 480

Query: 481 PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV 540
           PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV
Sbjct: 481 PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV 540

Query: 541 KSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV 600
           KSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV
Sbjct: 541 KSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV 600

Query: 601 RFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVTNV 660
           RFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVTNV
Sbjct: 601 RFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVTNV 660

Query: 661 GSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQ 720
           GSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQ
Sbjct: 661 GSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQ 720

Query: 721 HKVRSPIIVFDSNKFIN 737
           HKVRSPIIVFDSNKFIN
Sbjct: 721 HKVRSPIIVFDSNKFIN 737

BLAST of Csor.00g308070 vs. NCBI nr
Match: XP_023550545.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1404 bits (3634), Expect = 0.0
Identity = 702/737 (95.25%), Postives = 717/737 (97.29%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLSRLLFLAFC S+LFFSSNSEH+DRKTYIVYMGSHPK+RV TRSHHVRMLQETIGSS
Sbjct: 1   MSSLSRLLFLAFCFSRLFFSSNSEHEDRKTYIVYMGSHPKDRVSTRSHHVRMLQETIGSS 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVF NGKKQLHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
           ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPP KWKGSCEVSSDFSCNNKIIGARS
Sbjct: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRTNGQYPINDIQGPRDSNGHGTHAASTVAG LVRQASMLGLGSGTARGGVPSARIASYK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM
Sbjct: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGNSGPKSFTVRNFSPWSLSVAASTTDRK L+GVQLGDGRSFDGVT+NTFDLNGTQYPL
Sbjct: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKFLSGVQLGDGRSFDGVTINTFDLNGTQYPL 360

Query: 361 VYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNN 420
           VYAGNIPN+GFNGSISRFCLTNSVDKE VKGKIVLCDFF+SPTNLSFLEGAIGIIMQDNN
Sbjct: 361 VYAGNIPNIGFNGSISRFCLTNSVDKELVKGKIVLCDFFVSPTNLSFLEGAIGIIMQDNN 420

Query: 421 PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN 480
           PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYK TPFVASFSSRGPN
Sbjct: 421 PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKETPFVASFSSRGPN 480

Query: 481 PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV 540
           PITPDILKPDLSGPGVEILAAWSPIGPPSGAE+DTRQLLFNIISGTSMSCPHATAVAAYV
Sbjct: 481 PITPDILKPDLSGPGVEILAAWSPIGPPSGAEDDTRQLLFNIISGTSMSCPHATAVAAYV 540

Query: 541 KSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV 600
           KSFHPSWSPAALKSALMTTAFPMR+DLNPDAEFAYGSGHINPL AVNPGLIYNATEIDYV
Sbjct: 541 KSFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEIDYV 600

Query: 601 RFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVTNV 660
           RFLCGQGYSTKL+QQVSGDNSSCSR D D+VFDLNYPSFALST ISTSISQVYRR+VTNV
Sbjct: 601 RFLCGQGYSTKLVQQVSGDNSSCSRGDIDLVFDLNYPSFALSTSISTSISQVYRRRVTNV 660

Query: 661 GSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQ 720
           GSANS YKA VSGP GL ITVNPSVLSFKALGEEL FEVTIEGSISSSIAS SLVWDDG+
Sbjct: 661 GSANSTYKAIVSGPLGLNITVNPSVLSFKALGEELRFEVTIEGSISSSIASGSLVWDDGK 720

Query: 721 HKVRSPIIVFDSNKFIN 737
           HKV+SPI+VFD N FIN
Sbjct: 721 HKVKSPIVVFDENTFIN 737

BLAST of Csor.00g308070 vs. NCBI nr
Match: XP_022922127.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1402 bits (3628), Expect = 0.0
Identity = 703/737 (95.39%), Postives = 716/737 (97.15%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLSRLLFLAFC SQLFF SNSEHDDRKTYIVYMGSHPK+RVLTRSHHVRMLQETIGS 
Sbjct: 1   MSSLSRLLFLAFCFSQLFFCSNSEHDDRKTYIVYMGSHPKDRVLTRSHHVRMLQETIGSH 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVF NGKKQLHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
           ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPP KWKGSCEVSSDFSCNNKIIGARS
Sbjct: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRTNGQYPINDIQGPRDSNGHGTHAASTVAG LVRQASMLGLGSGTARGGVPSARIASYK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DAD+LAAFDDAIADGVDIISFSVGG+ PRDYFNDSIAIGAFHAMKK ILTSM
Sbjct: 241 ICWSDGCPDADILAAFDDAIADGVDIISFSVGGQTPRDYFNDSIAIGAFHAMKKEILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGN+GPKSFTVRNFSPWSLSVAASTTDRK LTGVQLGDGRSF+GVT+NTFDLNGTQYPL
Sbjct: 301 SAGNNGPKSFTVRNFSPWSLSVAASTTDRKFLTGVQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNN 420
           VYAGNIPN+GFNGSISRFCLTNSVDKESVKGKIVLCDFF+ PTNL+FLEGAIGIIMQDNN
Sbjct: 361 VYAGNIPNIGFNGSISRFCLTNSVDKESVKGKIVLCDFFVLPTNLNFLEGAIGIIMQDNN 420

Query: 421 PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN 480
           PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN
Sbjct: 421 PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN 480

Query: 481 PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV 540
           PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV
Sbjct: 481 PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV 540

Query: 541 KSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV 600
           K+FHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV
Sbjct: 541 KTFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV 600

Query: 601 RFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVTNV 660
           RFLCGQGYSTKLIQQVSGDNSSCSRSDS+VVFDLNYPSFALST IST ISQVYRR+VTNV
Sbjct: 601 RFLCGQGYSTKLIQQVSGDNSSCSRSDSNVVFDLNYPSFALSTSISTPISQVYRRRVTNV 660

Query: 661 GSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQ 720
           GSANS Y A VSGPS LKITVNPSVLSFKALGEELSFEVTIEGSISS I SASLVWDDGQ
Sbjct: 661 GSANSTYNAIVSGPSRLKITVNPSVLSFKALGEELSFEVTIEGSISSGIVSASLVWDDGQ 720

Query: 721 HKVRSPIIVFDSNKFIN 737
           HKVRSP+IVFDS  FIN
Sbjct: 721 HKVRSPVIVFDSTSFIN 737

BLAST of Csor.00g308070 vs. NCBI nr
Match: XP_022922167.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1342 bits (3473), Expect = 0.0
Identity = 669/739 (90.53%), Postives = 701/739 (94.86%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLSRLLFLAF LS+LFFSSNSE DD++TYIVYMGSHPK+RV TRSHHVRMLQETIGSS
Sbjct: 1   MSSLSRLLFLAFWLSRLFFSSNSEDDDKRTYIVYMGSHPKDRVSTRSHHVRMLQETIGSS 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           FAPHSLLHSY+RSFNGFV KLTE EV+ +SEMKGVISVF N KKQLHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYQRSFNGFVVKLTEDEVKIISEMKGVISVFPNEKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
             RVPSVESDIIVGVLDTGIWPESPSFLD+GYGPPPP+WKGSCE S +FSCNNKIIGARS
Sbjct: 121 VGRVPSVESDIIVGVLDTGIWPESPSFLDEGYGPPPPRWKGSCEASLNFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRTNGQYPINDIQGPRDSNGHGTH ASTVAG LVRQASMLGLGSGTARGGVPSARIASYK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHTASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSD C DAD+LAAFDDAIADGVDIISFSVGG  P+DYFNDS+AIGAFHAMKKGILTSM
Sbjct: 241 ICWSDDCSDADILAAFDDAIADGVDIISFSVGGHTPKDYFNDSMAIGAFHAMKKGILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGN GP+SFT+RNFSPWSLSVAASTT+R  L+G+QLGDGRSF+GVT+NTFDLNGTQYPL
Sbjct: 301 SAGNDGPQSFTIRNFSPWSLSVAASTTNRSYLSGIQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGNIPNV--GFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQD 420
           VYAGNIPN+  GFNGSISRFCL NSVD+ESVKGKIVLCDFF+SP NL  LEGAIGIIMQD
Sbjct: 361 VYAGNIPNITGGFNGSISRFCLPNSVDRESVKGKIVLCDFFVSPKNLGSLEGAIGIIMQD 420

Query: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480
            NPKDLTFPFPLPASHLGTQEGALISSYANLT LPTATILKSTEGKYKATPFVASFSSRG
Sbjct: 421 TNPKDLTFPFPLPASHLGTQEGALISSYANLTGLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540
           PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600
           YVK+FHPSWSPAALKSALMTTAFPMR+DLNPDAEFAYGSGHINPL AVNPGLIYNATEID
Sbjct: 541 YVKTFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEID 600

Query: 601 YVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVT 660
           YVRFLCGQGYSTKL+QQVSGDNSSCSR D D+VFDLNYPSFALST ISTSISQVYRR+VT
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSCSRGDIDLVFDLNYPSFALSTSISTSISQVYRRRVT 660

Query: 661 NVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDD 720
           NVGSANS YKA VSGPSGL ITVNPSVLSFKALGEELSFE+TIEGSISSSIASASLVWDD
Sbjct: 661 NVGSANSTYKAIVSGPSGLNITVNPSVLSFKALGEELSFELTIEGSISSSIASASLVWDD 720

Query: 721 GQHKVRSPIIVFDSNKFIN 737
           G+HKV+SPI+VFD N FIN
Sbjct: 721 GKHKVKSPIVVFDENTFIN 739

BLAST of Csor.00g308070 vs. NCBI nr
Match: XP_022973015.1 (cucumisin-like [Cucurbita maxima])

HSP 1 Score: 1305 bits (3378), Expect = 0.0
Identity = 655/737 (88.87%), Postives = 685/737 (92.94%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLSRLLFLAF LS LFF+SNSEHD+R TYIVYMGSHPK+RV TRSHH RML+E IGSS
Sbjct: 1   MSSLSRLLFLAFFLSLLFFASNSEHDNRTTYIVYMGSHPKDRVSTRSHHERMLEEAIGSS 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           F   SLLHSY++SFNGFV KLTE EVQKVSEMKGVISVF NGKKQLHTTRSWDFMGLSQQ
Sbjct: 61  FTRGSLLHSYKKSFNGFVVKLTEDEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
            SRVP VESDIIVGVLDTGIWPESPSFLD+GYGPPPPKWKGSCE S +FSCNNKIIGARS
Sbjct: 121 VSRVPLVESDIIVGVLDTGIWPESPSFLDEGYGPPPPKWKGSCETSLNFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRT+G Y I DIQGP DSNGHGTH ASTVAG LVRQASMLGLG+GTARGGVPSARIASYK
Sbjct: 181 YRTSGNYAIGDIQGPIDSNGHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DAD+LAAFDDAIADGVDIISFSVGG  P +YFNDSIAIGAFHAMK GILTSM
Sbjct: 241 ICWSDGCSDADILAAFDDAIADGVDIISFSVGGHTPNNYFNDSIAIGAFHAMKNGILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGN G K FT+RNFSPWSLSVAASTTDR+ L+ VQLGDGRSFDGVT+NTFDLNGTQYPL
Sbjct: 301 SAGNDGWKPFTIRNFSPWSLSVAASTTDRRFLSKVQLGDGRSFDGVTINTFDLNGTQYPL 360

Query: 361 VYAGNIPNV--GFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQD 420
           VYAGNIPNV  GFNGSISRFCL NSVD + VKGKIVLCD F+SP    FLEGAIGIIMQD
Sbjct: 361 VYAGNIPNVSAGFNGSISRFCLRNSVDSDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQD 420

Query: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480
           N PKDLTFPFPLPASHLGTQ+GALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG
Sbjct: 421 NRPKDLTFPFPLPASHLGTQQGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540
           PNPITPDILKPDLSGPGVEILAAW+P+GPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWTPLGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600
           YVKSFHPSWSPAALKSALMTTAFPMR+DLNPDAEFAYGSGHINPLGAVNPGLIYNATEID
Sbjct: 541 YVKSFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600

Query: 601 YVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVT 660
           YVRFLCGQGYSTKL+QQVSGDNSSCSR D D+VFDLNYPSFALSTP+STSISQVYRR+VT
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSCSRGDFDLVFDLNYPSFALSTPMSTSISQVYRRRVT 660

Query: 661 NVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDD 720
           NVGSANS YKA VSGPSGLKIT+NPSVLSFKALGEELSFE+TIEGSIS  IASASLVWDD
Sbjct: 661 NVGSANSTYKAIVSGPSGLKITLNPSVLSFKALGEELSFELTIEGSISRGIASASLVWDD 720

Query: 721 GQHKVRSPIIVFDSNKF 735
           GQH V+SPI+VFD N F
Sbjct: 721 GQHNVKSPIVVFDPNTF 737

BLAST of Csor.00g308070 vs. ExPASy TrEMBL
Match: A0A6J1E2C5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1)

HSP 1 Score: 1402 bits (3628), Expect = 0.0
Identity = 703/737 (95.39%), Postives = 716/737 (97.15%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLSRLLFLAFC SQLFF SNSEHDDRKTYIVYMGSHPK+RVLTRSHHVRMLQETIGS 
Sbjct: 1   MSSLSRLLFLAFCFSQLFFCSNSEHDDRKTYIVYMGSHPKDRVLTRSHHVRMLQETIGSH 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVF NGKKQLHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
           ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPP KWKGSCEVSSDFSCNNKIIGARS
Sbjct: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRTNGQYPINDIQGPRDSNGHGTHAASTVAG LVRQASMLGLGSGTARGGVPSARIASYK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DAD+LAAFDDAIADGVDIISFSVGG+ PRDYFNDSIAIGAFHAMKK ILTSM
Sbjct: 241 ICWSDGCPDADILAAFDDAIADGVDIISFSVGGQTPRDYFNDSIAIGAFHAMKKEILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGN+GPKSFTVRNFSPWSLSVAASTTDRK LTGVQLGDGRSF+GVT+NTFDLNGTQYPL
Sbjct: 301 SAGNNGPKSFTVRNFSPWSLSVAASTTDRKFLTGVQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQDNN 420
           VYAGNIPN+GFNGSISRFCLTNSVDKESVKGKIVLCDFF+ PTNL+FLEGAIGIIMQDNN
Sbjct: 361 VYAGNIPNIGFNGSISRFCLTNSVDKESVKGKIVLCDFFVLPTNLNFLEGAIGIIMQDNN 420

Query: 421 PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN 480
           PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN
Sbjct: 421 PKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRGPN 480

Query: 481 PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV 540
           PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV
Sbjct: 481 PITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAAYV 540

Query: 541 KSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV 600
           K+FHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV
Sbjct: 541 KTFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEIDYV 600

Query: 601 RFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVTNV 660
           RFLCGQGYSTKLIQQVSGDNSSCSRSDS+VVFDLNYPSFALST IST ISQVYRR+VTNV
Sbjct: 601 RFLCGQGYSTKLIQQVSGDNSSCSRSDSNVVFDLNYPSFALSTSISTPISQVYRRRVTNV 660

Query: 661 GSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDDGQ 720
           GSANS Y A VSGPS LKITVNPSVLSFKALGEELSFEVTIEGSISS I SASLVWDDGQ
Sbjct: 661 GSANSTYNAIVSGPSRLKITVNPSVLSFKALGEELSFEVTIEGSISSGIVSASLVWDDGQ 720

Query: 721 HKVRSPIIVFDSNKFIN 737
           HKVRSP+IVFDS  FIN
Sbjct: 721 HKVRSPVIVFDSTSFIN 737

BLAST of Csor.00g308070 vs. ExPASy TrEMBL
Match: A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)

HSP 1 Score: 1342 bits (3473), Expect = 0.0
Identity = 669/739 (90.53%), Postives = 701/739 (94.86%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLSRLLFLAF LS+LFFSSNSE DD++TYIVYMGSHPK+RV TRSHHVRMLQETIGSS
Sbjct: 1   MSSLSRLLFLAFWLSRLFFSSNSEDDDKRTYIVYMGSHPKDRVSTRSHHVRMLQETIGSS 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           FAPHSLLHSY+RSFNGFV KLTE EV+ +SEMKGVISVF N KKQLHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYQRSFNGFVVKLTEDEVKIISEMKGVISVFPNEKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
             RVPSVESDIIVGVLDTGIWPESPSFLD+GYGPPPP+WKGSCE S +FSCNNKIIGARS
Sbjct: 121 VGRVPSVESDIIVGVLDTGIWPESPSFLDEGYGPPPPRWKGSCEASLNFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRTNGQYPINDIQGPRDSNGHGTH ASTVAG LVRQASMLGLGSGTARGGVPSARIASYK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHTASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSD C DAD+LAAFDDAIADGVDIISFSVGG  P+DYFNDS+AIGAFHAMKKGILTSM
Sbjct: 241 ICWSDDCSDADILAAFDDAIADGVDIISFSVGGHTPKDYFNDSMAIGAFHAMKKGILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGN GP+SFT+RNFSPWSLSVAASTT+R  L+G+QLGDGRSF+GVT+NTFDLNGTQYPL
Sbjct: 301 SAGNDGPQSFTIRNFSPWSLSVAASTTNRSYLSGIQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGNIPNV--GFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQD 420
           VYAGNIPN+  GFNGSISRFCL NSVD+ESVKGKIVLCDFF+SP NL  LEGAIGIIMQD
Sbjct: 361 VYAGNIPNITGGFNGSISRFCLPNSVDRESVKGKIVLCDFFVSPKNLGSLEGAIGIIMQD 420

Query: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480
            NPKDLTFPFPLPASHLGTQEGALISSYANLT LPTATILKSTEGKYKATPFVASFSSRG
Sbjct: 421 TNPKDLTFPFPLPASHLGTQEGALISSYANLTGLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540
           PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600
           YVK+FHPSWSPAALKSALMTTAFPMR+DLNPDAEFAYGSGHINPL AVNPGLIYNATEID
Sbjct: 541 YVKTFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEID 600

Query: 601 YVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVT 660
           YVRFLCGQGYSTKL+QQVSGDNSSCSR D D+VFDLNYPSFALST ISTSISQVYRR+VT
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSCSRGDIDLVFDLNYPSFALSTSISTSISQVYRRRVT 660

Query: 661 NVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDD 720
           NVGSANS YKA VSGPSGL ITVNPSVLSFKALGEELSFE+TIEGSISSSIASASLVWDD
Sbjct: 661 NVGSANSTYKAIVSGPSGLNITVNPSVLSFKALGEELSFELTIEGSISSSIASASLVWDD 720

Query: 721 GQHKVRSPIIVFDSNKFIN 737
           G+HKV+SPI+VFD N FIN
Sbjct: 721 GKHKVKSPIVVFDENTFIN 739

BLAST of Csor.00g308070 vs. ExPASy TrEMBL
Match: A0A6J1IAA2 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111471532 PE=3 SV=1)

HSP 1 Score: 1305 bits (3378), Expect = 0.0
Identity = 655/737 (88.87%), Postives = 685/737 (92.94%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLSRLLFLAF LS LFF+SNSEHD+R TYIVYMGSHPK+RV TRSHH RML+E IGSS
Sbjct: 1   MSSLSRLLFLAFFLSLLFFASNSEHDNRTTYIVYMGSHPKDRVSTRSHHERMLEEAIGSS 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           F   SLLHSY++SFNGFV KLTE EVQKVSEMKGVISVF NGKKQLHTTRSWDFMGLSQQ
Sbjct: 61  FTRGSLLHSYKKSFNGFVVKLTEDEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
            SRVP VESDIIVGVLDTGIWPESPSFLD+GYGPPPPKWKGSCE S +FSCNNKIIGARS
Sbjct: 121 VSRVPLVESDIIVGVLDTGIWPESPSFLDEGYGPPPPKWKGSCETSLNFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRT+G Y I DIQGP DSNGHGTH ASTVAG LVRQASMLGLG+GTARGGVPSARIASYK
Sbjct: 181 YRTSGNYAIGDIQGPIDSNGHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DAD+LAAFDDAIADGVDIISFSVGG  P +YFNDSIAIGAFHAMK GILTSM
Sbjct: 241 ICWSDGCSDADILAAFDDAIADGVDIISFSVGGHTPNNYFNDSIAIGAFHAMKNGILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGN G K FT+RNFSPWSLSVAASTTDR+ L+ VQLGDGRSFDGVT+NTFDLNGTQYPL
Sbjct: 301 SAGNDGWKPFTIRNFSPWSLSVAASTTDRRFLSKVQLGDGRSFDGVTINTFDLNGTQYPL 360

Query: 361 VYAGNIPNV--GFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQD 420
           VYAGNIPNV  GFNGSISRFCL NSVD + VKGKIVLCD F+SP    FLEGAIGIIMQD
Sbjct: 361 VYAGNIPNVSAGFNGSISRFCLRNSVDSDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQD 420

Query: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480
           N PKDLTFPFPLPASHLGTQ+GALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG
Sbjct: 421 NRPKDLTFPFPLPASHLGTQQGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540
           PNPITPDILKPDLSGPGVEILAAW+P+GPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWTPLGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600
           YVKSFHPSWSPAALKSALMTTAFPMR+DLNPDAEFAYGSGHINPLGAVNPGLIYNATEID
Sbjct: 541 YVKSFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600

Query: 601 YVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVT 660
           YVRFLCGQGYSTKL+QQVSGDNSSCSR D D+VFDLNYPSFALSTP+STSISQVYRR+VT
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSCSRGDFDLVFDLNYPSFALSTPMSTSISQVYRRRVT 660

Query: 661 NVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDD 720
           NVGSANS YKA VSGPSGLKIT+NPSVLSFKALGEELSFE+TIEGSIS  IASASLVWDD
Sbjct: 661 NVGSANSTYKAIVSGPSGLKITLNPSVLSFKALGEELSFELTIEGSISRGIASASLVWDD 720

Query: 721 GQHKVRSPIIVFDSNKF 735
           GQH V+SPI+VFD N F
Sbjct: 721 GQHNVKSPIVVFDPNTF 737

BLAST of Csor.00g308070 vs. ExPASy TrEMBL
Match: A0A6J1E6I3 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430414 PE=3 SV=1)

HSP 1 Score: 1287 bits (3331), Expect = 0.0
Identity = 646/737 (87.65%), Postives = 679/737 (92.13%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60
           MSSLS LLFLAFCLS LF  SNS+ DDR TYIVYMGSHPK+R  TR+HH RML E IGSS
Sbjct: 1   MSSLSSLLFLAFCLSLLFLGSNSKDDDRTTYIVYMGSHPKDRGSTRAHHERMLGEAIGSS 60

Query: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120
           FAPHSLLHSY+RSFNGFVAKLTE EVQKVSEMKGVISVF NGKKQLHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYKRSFNGFVAKLTEEEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180
            SRVPSVESDIIVGVLDTGIWP SPSFLD+GYGPPPPKWKGSCE S +FSCNNKIIGARS
Sbjct: 121 VSRVPSVESDIIVGVLDTGIWPNSPSFLDEGYGPPPPKWKGSCEASLNFSCNNKIIGARS 180

Query: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240
           YRT+G Y I DIQGP DSNGHGTH ASTVAG LVRQASMLGLG+GTARGGVPSARIASYK
Sbjct: 181 YRTSGNYAIGDIQGPIDSNGHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYK 240

Query: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC +AD+LAAFDD IADGVDIIS SVGG  P +YFNDSIAIGAFHAMK GILTSM
Sbjct: 241 ICWSDGCPEADILAAFDDVIADGVDIISLSVGGHTPNNYFNDSIAIGAFHAMKNGILTSM 300

Query: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360
           SAGN G K FT+RNFSPWSLSVA+STT+R+ L+ VQLGDGRSF GVT+NTFDLNGTQY L
Sbjct: 301 SAGNDGWKPFTIRNFSPWSLSVASSTTNRRFLSKVQLGDGRSFHGVTINTFDLNGTQYSL 360

Query: 361 VYAGNIPNV--GFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQD 420
           VYAGNIPNV  GFNGS+SRFC  NSVD++ VKGKIVLCD F+SP    FLEGAIGIIMQD
Sbjct: 361 VYAGNIPNVTAGFNGSVSRFCTRNSVDRDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQD 420

Query: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480
           NNPKDL+ PFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG
Sbjct: 421 NNPKDLSSPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540
           PNPITPDILKPDLSGPGVEILAAW+P+GPPSGA+EDTRQLLFNIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWTPLGPPSGAKEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600
           YVKSFHPSWSPAALKSALMTTAFPMR++LNP+AEFAYGSGHINPL AVNPGLIYNATEID
Sbjct: 541 YVKSFHPSWSPAALKSALMTTAFPMRAELNPEAEFAYGSGHINPLSAVNPGLIYNATEID 600

Query: 601 YVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQVYRRKVT 660
           YVRFLCGQGYSTKL+QQVSGDNSSCSR D D+VFDLNYPSFALSTPISTSISQVYRR+VT
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSCSRGDFDLVFDLNYPSFALSTPISTSISQVYRRRVT 660

Query: 661 NVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWDD 720
           NVGSANS Y A VSGPSGLKITVNPSVLSF ALGEELSFEVTIEGSISSSIASASLVWDD
Sbjct: 661 NVGSANSTYNAIVSGPSGLKITVNPSVLSFNALGEELSFEVTIEGSISSSIASASLVWDD 720

Query: 721 GQHKVRSPIIVFDSNKF 735
           G HKV+SPI+VFD N F
Sbjct: 721 GHHKVKSPIVVFDPNTF 737

BLAST of Csor.00g308070 vs. ExPASy TrEMBL
Match: A0A1S3AUC4 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)

HSP 1 Score: 1145 bits (2963), Expect = 0.0
Identity = 579/744 (77.82%), Postives = 646/744 (86.83%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDD--RKTYIVYMGSHPKERV----LTRSHHVRMLQ 60
           MSSLSRLLFL FC S LFFSS SE DD  RKTYIVYMGSHPK++V    L+  HH+R+LQ
Sbjct: 1   MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60

Query: 61  ETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDF 120
           E IGS+FAPH LLHSY+RSFNGFVAKLTE E +KVSEM+GVISVF N + QLHTTRSWDF
Sbjct: 61  EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120

Query: 121 MGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNK 180
           MG S+Q  RVPSVESDIIVGV DTGIWPESPSFLD GYGPPPPKWKGSCEVS +FSCNNK
Sbjct: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180

Query: 181 IIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSA 240
           IIGA+SYR++G+YPI+DI+GPRDSNGHGTHAASTVAG LVRQASMLGLG+GTARGGVPSA
Sbjct: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240

Query: 241 RIASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKP-RDYFNDSIAIGAFHAMK 300
           RIA+YK+CWSD C  ADVLAAFDDAIADGVDIIS SVG ++P  +YFND IAIG FHAM+
Sbjct: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300

Query: 301 KGILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDL 360
            GILTS SAGN GP+ FT+ NFSPW+LSVAASTTDR+ LT VQLGDGR F+GVT+NTFDL
Sbjct: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360

Query: 361 NGTQYPLVYAGNIPNV--GFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGA 420
           NGTQYPLV+AGNIPNV  GFNGSISRFCL NSVD+E VKGKI +CD  + P+++  LE A
Sbjct: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420

Query: 421 IGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFV 480
           +GIIMQD +PKDLT  FPLPASHLGTQ+  LISSY NLT +PTATILKSTE K +A P V
Sbjct: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480

Query: 481 ASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCP 540
           ASFSSRGPNP TPDILKPD+ GPGVEILAAWSPI  PS A+ D R+LLFNIISGTSM+CP
Sbjct: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540

Query: 541 HATAVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLI 600
           HATAVAAYVKSFHPSWSPAALKSAL+TTAFPMR DL P+AEFAYGSGHINPLGAVNPGLI
Sbjct: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600

Query: 601 YNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPISTSISQ 660
           YNA+EIDY+RFLCG+GY+T L++ ++ DNS+CS ++S +V+DLNY SFAL T IST  SQ
Sbjct: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFSQ 660

Query: 661 VYRRKVTNVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIAS 720
           VY+R+VTNVGS NS YKAT+  PS L ITVNPS LSFKAL EEL FEVTIEG I+ SIAS
Sbjct: 661 VYKRRVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIAS 720

Query: 721 ASLVWDDGQHKVRSPIIVFDSNKF 735
           ASLVWDDG HKVRSPIIVFDS+ F
Sbjct: 721 ASLVWDDGVHKVRSPIIVFDSDTF 744

BLAST of Csor.00g308070 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 665.2 bits (1715), Expect = 6.0e-191
Identity = 358/746 (47.99%), Postives = 495/746 (66.35%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSS-NSEHDDRKTYIVYMGSHPKERVLT-RSHHVRMLQETIG 60
           M++L+    L  CL  LF SS ++  DD++ YIVYMGS       T  S H+ +LQE  G
Sbjct: 1   MATLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 60

Query: 61  SSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLS 120
            S     L+ SY+RSFNGF A+LTE E ++V++M GV+SVF N K QL TT SWDFMGL 
Sbjct: 61  ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 120

Query: 121 Q--QASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKII 180
           +  +  R P+VESD I+GV+D+GI PES SF D+G+GPPP KWKG C    +F+CNNK+I
Sbjct: 121 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 180

Query: 181 GARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARI 240
           GAR Y +         +G RD +GHGTH AST AG  V  AS  G+G+GT RGGVP++R+
Sbjct: 181 GARDYTS---------EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRV 240

Query: 241 ASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGI 300
           A+YK+C   GC    +L+AFDDAIADGVD+I+ S+G +    + ND IAIGAFHAM KG+
Sbjct: 241 AAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGV 300

Query: 301 LTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGT 360
           LT  SAGNSGPK  +V   +PW L+VAASTT+R  +T V LG+G++  G +VN +++ G 
Sbjct: 301 LTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGK 360

Query: 361 QYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLE--GAIGI 420
            YPLVY  +  +   +   +  C  + VDK  VKGKI++C     P  L  +E  GA+G+
Sbjct: 361 DYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG---GPGGLKIVESVGAVGL 420

Query: 421 IMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASF 480
           I +   P D+ F  PLPA+ L T++   + SY   T  P A +LK+     + +P +ASF
Sbjct: 421 IYRTPKP-DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASF 480

Query: 481 SSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHAT 540
           SSRGPN I  DILKPD++ PGVEILAA+SP G PS  ++DTR + ++++SGTSMSCPH  
Sbjct: 481 SSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVA 540

Query: 541 AVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLN--PDAEFAYGSGHINPLGAVNPGLIY 600
            VAAYVK+F+P WSP+ ++SA+MTTA+P+ +        EFAYGSGH++P+ A NPGL+Y
Sbjct: 541 GVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVY 600

Query: 601 NATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSFALSTPIS-TSISQ 660
              + D++ FLCG  Y++++++ +SG+  +CS +   +  +LNYPS +     S T+ + 
Sbjct: 601 ELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTV 660

Query: 661 VYRRKVTNVGSANSIYKATVSGPSG--LKITVNPSVLSFKALGEELSFEVTIEGS-ISSS 720
            + R +TNVG+ NS Y + V    G  L + + PSVLSFK + E+ SF VT+ GS + S 
Sbjct: 661 TFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSE 720

Query: 721 I-ASASLVWDDGQHKVRSPIIVFDSN 734
           + +SA+L+W DG H VRSPI+V+ S+
Sbjct: 721 VPSSANLIWSDGTHNVRSPIVVYTSD 731

BLAST of Csor.00g308070 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 658.3 bits (1697), Expect = 7.3e-189
Identity = 346/701 (49.36%), Postives = 462/701 (65.91%), Query Frame = 0

Query: 35  MGSHPKERVLTRSHHVRMLQETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKG 94
           MG+ P+ +    SHH+ +LQ+ +G+  A H L+ SY+RSFNGF A L++ E QK+  MK 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 95  VISVFLNGKKQLHTTRSWDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGP 154
           V+SVF +   +L TTRSWDF+G  ++A R    ESD+IVGV+D+GIWPES SF D+G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 155 PPPKWKGSCEVSSDFSCNNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELV 214
           PP KWKGSC+    F+CNNK+IGAR Y              RD  GHGTH AST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTAAGNAV 180

Query: 215 RQASMLGLGSGTARGGVPSARIASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGR 274
           + AS  GL  GTARGGVPSARIA+YK+C+ + C D D+LAAFDDAIADGVD+IS S+   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 275 KPRDYFNDSIAIGAFHAMKKGILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTG 334
              +  N S+AIG+FHAM +GI+T+ SAGN+GP   +V N SPW ++VAAS TDR+ +  
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 335 VQLGDGRSFDGVTVNTFDLNGTQYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIV 394
           V LG+G++  G++VNTF+LNGT++P+VY  N+     + + + +C +  VD E VKGKIV
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIV 360

Query: 395 LCDFFISPTNLSFLEGAIGIIMQDNNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPT 454
           LCD F+     ++L GAIG+I+Q+    D  F  P PAS LG ++   I SY      P 
Sbjct: 361 LCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQ 420

Query: 455 ATILKSTEGKYKATPFVASFSSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSG--AE 514
           A IL++ E   +  P+V SFSSRGP+ +  ++LKPD+S PG+EILAA+SP+  PS     
Sbjct: 421 AEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNP 480

Query: 515 EDTRQLLFNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAE 574
           ED R + ++++SGTSM+CPH   VAAYVKSFHP WSP+A+KSA+MTTA PM    NP+ E
Sbjct: 481 EDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQE 540

Query: 575 FAYGSGHINPLGAVNPGLIYNATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVF 634
           FAYGSG INP  A +PGL+Y     DY++ LC +G+ +  +   SG N +C  S+   V 
Sbjct: 541 FAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTC--SERTEVK 600

Query: 635 DLNYPSFALSTPISTSISQVYRRKVTNVGSANSIYKATVSG-PSGLKITVNPSVLSFKAL 694
           DLNYP+           +  ++R VTNVG  NS YKA+V      L+I++ P +L F  L
Sbjct: 601 DLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFL 660

Query: 695 GEELSFEVTIEGS--ISSSIASASLVWDDGQHKVRSPIIVF 731
            E+ SF VTI G      S  S+S+VW DG H VRSPI+ +
Sbjct: 661 EEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 689

BLAST of Csor.00g308070 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 648.3 bits (1671), Expect = 7.5e-186
Identity = 348/743 (46.84%), Postives = 485/743 (65.28%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSE--HDDRKTYIVYMGS-HPKERVLTRSHHVRMLQETI 60
           M++L+    L   L  L  SS S    +D + YIVYMGS   +   +  S H+ +LQ+  
Sbjct: 1   MANLAASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVT 60

Query: 61  GSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGL 120
           G S     L+ SY+RSFNGF A+LTE E   ++E++GV+SVF N   QLHTT SWDFMG+
Sbjct: 61  GESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGV 120

Query: 121 SQ--QASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKI 180
            +     R  ++ESD I+GV+DTGIWPES SF D+G+GPPP KWKG C    +F+CNNK+
Sbjct: 121 KEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKL 180

Query: 181 IGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSAR 240
           IGAR Y +         +G RD++GHGTH AST AG  V+  S  G+G+GT RGGVP++R
Sbjct: 181 IGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASR 240

Query: 241 IASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKG 300
           IA+YK+C   GC    +L++FDDAIADGVD+I+ S+G + P  + +D IAIGAFHAM KG
Sbjct: 241 IAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKG 300

Query: 301 ILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNG 360
           ILT  SAGNSGPK  TV + +PW  +VAASTT+R  +T V LG+G++  G +VN FD+ G
Sbjct: 301 ILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKG 360

Query: 361 TQYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGII 420
            +YPLVY  +  +   +   +  C    ++K  VKGKI++C     P+     +    I 
Sbjct: 361 KKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIA 420

Query: 421 MQDNNPK-DLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASF 480
           + D +P+ D+ F   LPAS L  ++   + SY      P A +LK+     + +P +ASF
Sbjct: 421 IIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASF 480

Query: 481 SSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHAT 540
           SSRGPN I  DILKPD++ PGVEILAA+SP G PS  E+DTR++ +++ SGTSM+CPH  
Sbjct: 481 SSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVA 540

Query: 541 AVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLN--PDAEFAYGSGHINPLGAVNPGLIY 600
            VAAYVK+F+P WSP+ ++SA+MTTA+P+++        EFAYG+GH++P+ A+NPGL+Y
Sbjct: 541 GVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVY 600

Query: 601 NATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSF-ALSTPISTSISQ 660
              + D++ FLCG  Y++K ++ +SGD   CS+ +  +  +LNYPS  A  +   ++ S 
Sbjct: 601 ELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSV 660

Query: 661 VYRRKVTNVGSANSIYKATVSGPSG--LKITVNPSVLSFKALGEELSFEVTIEGS-ISSS 720
            + R +TNVG+ NS YK+ V    G  L I V PSVL FK + E+ SF VT+ GS + S 
Sbjct: 661 TFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSE 720

Query: 721 I-ASASLVWDDGQHKVRSPIIVF 731
           + +SA+L+W DG H VRSPI+V+
Sbjct: 721 VPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Csor.00g308070 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 642.5 bits (1656), Expect = 4.1e-184
Identity = 347/741 (46.83%), Postives = 481/741 (64.91%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSE--HDDRKTYIVYMGS-HPKERVLTRSHHVRMLQETI 60
           M++L+    L   L  L  SS S    +D + YIVYMGS   +   +  S H+ +LQ+  
Sbjct: 1   MANLAASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVT 60

Query: 61  GSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGL 120
           G S     L+ SY+RSFNGF A+LTE E   ++E++GV+SVF N   QLHTT SWDFMG+
Sbjct: 61  GESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGV 120

Query: 121 SQ--QASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKI 180
            +     R  ++ESD I+GV+DTGIWPES SF D+G+GPPP KWKG C    +F+CNNK+
Sbjct: 121 KEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKL 180

Query: 181 IGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSAR 240
           IGAR Y +         +G RD++GHGTH AST AG  V+  S  G+G+GT RGGVP++R
Sbjct: 181 IGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASR 240

Query: 241 IASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKG 300
           IA+YK+C   GC    +L++FDDAIADGVD+I+ S+G + P  + +D IAIGAFHAM KG
Sbjct: 241 IAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKG 300

Query: 301 ILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNG 360
           ILT  SAGNSGPK  TV + +PW  +VAASTT+R  +T V LG+G++  G +VN FD+ G
Sbjct: 301 ILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKG 360

Query: 361 TQYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGII 420
            +YPLVY  +  +   +   +  C    ++K  VKGKI++C     P+     +    I 
Sbjct: 361 KKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIA 420

Query: 421 MQDNNPK-DLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASF 480
           + D +P+ D+ F   LPAS L  ++   + SY      P A +LK+     + +P +ASF
Sbjct: 421 IIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASF 480

Query: 481 SSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHAT 540
           SSRGPN I  DILKPD++ PGVEILAA+SP G PS  E+DTR++ +++ SGTSM+CPH  
Sbjct: 481 SSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVA 540

Query: 541 AVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNA 600
            VAAYVK+F+P WSP+ ++SA+MTTA   +       EFAYG+GH++P+ A+NPGL+Y  
Sbjct: 541 GVAAYVKTFYPRWSPSMIQSAIMTTA---KGRGIASTEFAYGAGHVDPMAALNPGLVYEL 600

Query: 601 TEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSF-ALSTPISTSISQVY 660
            + D++ FLCG  Y++K ++ +SGD   CS+ +  +  +LNYPS  A  +   ++ S  +
Sbjct: 601 DKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTF 660

Query: 661 RRKVTNVGSANSIYKATVSGPSG--LKITVNPSVLSFKALGEELSFEVTIEGS-ISSSI- 720
            R +TNVG+ NS YK+ V    G  L I V PSVL FK + E+ SF VT+ GS + S + 
Sbjct: 661 NRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVP 720

Query: 721 ASASLVWDDGQHKVRSPIIVF 731
           +SA+L+W DG H VRSPI+V+
Sbjct: 721 SSANLIWSDGTHNVRSPIVVY 724

BLAST of Csor.00g308070 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 642.5 bits (1656), Expect = 4.1e-184
Identity = 348/743 (46.84%), Postives = 483/743 (65.01%), Query Frame = 0

Query: 1   MSSLSRLLFLAFCLSQLFFSSNSE--HDDRKTYIVYMGS-HPKERVLTRSHHVRMLQETI 60
           M++L+    L   L  L  SS S    +D + YIVYMGS   +   +  S H+ +LQ+  
Sbjct: 1   MANLAASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVT 60

Query: 61  GSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGL 120
           G S     L+ SY+RSFNGF A+LTE E   ++E  GV+SVF N   QLHTT SWDFMG+
Sbjct: 61  GESSIEGRLVRSYKRSFNGFAARLTESERTLIAE--GVVSVFPNKILQLHTTTSWDFMGV 120

Query: 121 SQ--QASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKI 180
            +     R  ++ESD I+GV+DTGIWPES SF D+G+GPPP KWKG C    +F+CNNK+
Sbjct: 121 KEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKL 180

Query: 181 IGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSAR 240
           IGAR Y +         +G RD++GHGTH AST AG  V+  S  G+G+GT RGGVP++R
Sbjct: 181 IGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASR 240

Query: 241 IASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKG 300
           IA+YK+C   GC    +L++FDDAIADGVD+I+ S+G + P  + +D IAIGAFHAM KG
Sbjct: 241 IAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKG 300

Query: 301 ILTSMSAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNG 360
           ILT  SAGNSGPK  TV + +PW  +VAASTT+R  +T V LG+G++  G +VN FD+ G
Sbjct: 301 ILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKG 360

Query: 361 TQYPLVYAGNIPNVGFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGII 420
            +YPLVY  +  +   +   +  C    ++K  VKGKI++C     P+     +    I 
Sbjct: 361 KKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIA 420

Query: 421 MQDNNPK-DLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASF 480
           + D +P+ D+ F   LPAS L  ++   + SY      P A +LK+     + +P +ASF
Sbjct: 421 IIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASF 480

Query: 481 SSRGPNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHAT 540
           SSRGPN I  DILKPD++ PGVEILAA+SP G PS  E+DTR++ +++ SGTSM+CPH  
Sbjct: 481 SSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVA 540

Query: 541 AVAAYVKSFHPSWSPAALKSALMTTAFPMRSDLN--PDAEFAYGSGHINPLGAVNPGLIY 600
            VAAYVK+F+P WSP+ ++SA+MTTA+P+++        EFAYG+GH++P+ A+NPGL+Y
Sbjct: 541 GVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVY 600

Query: 601 NATEIDYVRFLCGQGYSTKLIQQVSGDNSSCSRSDSDVVFDLNYPSF-ALSTPISTSISQ 660
              + D++ FLCG  Y++K ++ +SGD   CS+ +  +  +LNYPS  A  +   ++ S 
Sbjct: 601 ELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSV 660

Query: 661 VYRRKVTNVGSANSIYKATVSGPSG--LKITVNPSVLSFKALGEELSFEVTIEGS-ISSS 720
            + R +TNVG+ NS YK+ V    G  L I V PSVL FK + E+ SF VT+ GS + S 
Sbjct: 661 TFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSE 720

Query: 721 I-ASASLVWDDGQHKVRSPIIVF 731
           + +SA+L+W DG H VRSPI+V+
Sbjct: 721 VPSSANLIWSDGTHNVRSPIVVY 727

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q395472.7e-23656.56Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9FIF86.9e-19248.92Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9FIG28.4e-19047.99Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... [more]
Q8L7D21.1e-18446.84Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... [more]
Q9FGU32.4e-18147.18Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... [more]
Match NameE-valueIdentityDescription
KAG6579374.10.0100.00hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023550545.10.095.25cucumisin-like [Cucurbita pepo subsp. pepo][more]
XP_022922127.10.095.39cucumisin-like [Cucurbita moschata][more]
XP_022922167.10.090.53cucumisin-like [Cucurbita moschata][more]
XP_022973015.10.088.87cucumisin-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E2C50.095.39cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1[more]
A0A6J1E2G40.090.53cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1[more]
A0A6J1IAA20.088.87cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111471532 PE=3 SV=1[more]
A0A6J1E6I30.087.65cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430414 PE=3 SV=1[more]
A0A1S3AUC40.077.82cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59120.16.0e-19147.99subtilase 4.13 [more]
AT5G59190.17.3e-18949.36subtilase family protein [more]
AT5G59090.17.5e-18646.84subtilase 4.12 [more]
AT5G59090.24.1e-18446.83subtilase 4.12 [more]
AT5G59090.34.1e-18446.84subtilase 4.12 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 524..540
score: 48.46
coord: 197..210
score: 54.89
coord: 128..147
score: 32.38
NoneNo IPR availableGENE3D2.60.40.2310coord: 603..731
e-value: 5.8E-43
score: 147.7
NoneNo IPR availableGENE3D3.50.30.30coord: 328..464
e-value: 2.0E-185
score: 619.0
NoneNo IPR availablePANTHERPTHR10795:SF649PUTATIVE-RELATEDcoord: 19..730
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 111..586
score: 27.889879
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 335..457
e-value: 2.92707E-20
score: 85.1579
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 633..729
e-value: 8.7E-28
score: 96.4
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 129..577
e-value: 1.6E-44
score: 152.3
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 130..599
e-value: 2.0E-185
score: 619.0
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 106..590
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 30..106
e-value: 6.5E-13
score: 49.2
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 15..107
e-value: 8.2E-26
score: 92.3
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 19..730
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 525..535
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 104..560
e-value: 4.77333E-131
score: 388.88

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g308070.m01Csor.00g308070.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity