Csor.00g307290 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g307290
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	thermospermine synthase ACAULIS5-like
Location	Csor_Chr05: 1988120 .. 1991214 (+)
RNA-Seq Expression	Csor.00g307290
Synteny	Csor.00g307290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGGTATTTGATTTCGCTAATTAACTTCCAAATCTCTTCGTATTTCTCCGTTCTAATTCCATCGCCGATTCATTTGTAAATCCGTTTCTTCTCTTCAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATCTTCTGCGATTCGTGAAAATTTTCAATCGGATTCGTTTTTTTCTTTCCTCGTTGTAACTGTATGAGATGATCTGATTAAGGTTTGTGAGTGTAGGCATTGATGATCGACGGGAAGATGCAGAGCGCTGAAGCCGATGAATTCGTGTATCACGAATGCTTGATTCATCCCGCACTATTATGCCATTCCAAGTGCGTTTTCCTTTTGCACCATTATTTGTTCACATCTCCAAATTATTGGTTAAATTAATAAAAATATTAGTAAACTTTTTTTCTTTTCTTAACACTTCAACAAAAAATCTAAAACTATGATTATTTTATATCAAATACCTTTCCACTTTTAAATTATTTCAAAAGTACATTAAATTTTTTTTTATATAGATATCTTTTATTTTTAATAATATATTTAAAAAATATCCATAAATTTTTAAAAATAGCACTGATACAATTTTTATATAAAAAAATTTTAAAAAATTTAACAACAAAAACTCTTAATTTACAACTCATTGTTTTCCTCGTACTAATGGTAAAACAAAAAATAAGTGTTTTCTAAAAGTTAAAGAGTATTAGTGTTATTTTTTAAATGCAATAATAATCCCTTCTATTTATATCTTAATAATATTAATAATAATATTTTTAATTGTATTTTTTAAACATGTTTTAATTTTTTATAAGATAAAGTTAGACGTATTTTTATTAATTTAACCAAATGTCTTTAATATTTTTATTAATTTAATCCAAATGTTTTTACTTTTCTTTATTATTAAATGAAACATACAATAATATGATGAATAGAGGATTGAATCTCCCTCATGCCAACTAAGAAAAGTTCAATAACTTTCTATTCTACGATGATTTGACCATTTGGCTCATGTTTTCAAGATTAATTTAGGAAAAGAGTGAATAACCAATAGAAAATAAAAATTTATACCTTTACCTTCTCTTAAGAAAAAGAATTTTTAATAATAGCATGATAATCTGAATTATGGCTTTGGAATTAACTCAATATTATTATAATACAATTATTTATAGCCCGAAAACTGTATTCATAATGGGAGGTGGGGAAGGATCAGCAGCCAGAGAAGTCCTCAAACACAAATCAATAGAAAAAGTTGTCATGTGCGATATCGATCAGGTAATATTACTCGAACCTCTTCAATGTCGTTGAACAAAAAAGAATTATTACTAAATTTATTTAATTTTCATTCGACAAAACTATGTGTTGTTTGCAGGATGTAGTAGATTTCTGCCGTAAATATTTGACTGTGAACCAAGAGACATTTTGTCACAAAAAACTTCATCTTGTAATAAACGACGCCAAGTAAGTTAATTAATCATTTATTTATACATAAAATTTCTTCTCTAATTTGAAAGCACAAACTAAAAGAAAATACTTTGAAAAGGGCGGAATTGGAAAAGAGAAAGGAGAAATATGATATAATTATAGGAGACCTAGCAGACCCTGTTAAAGATGGGCCTTGCTACAAGCTCTACACCAAATCCTTCTACCAGGAGATCGTCAAGCCAAAGCTCCACGACAATGGCATCTTCGTAACTCAGGTTTCTCAACCTTATTATACATAAACTGCATTATTCATTTAATTTTTTTTACAATATAGTTTTTCGATAACTCTAAGGTTTGCAGGCAGGACCAGCCGGAATTTTCACCCACAAAGAGGTTTTTACCTCCATATATAACACAATCAAACAGACATTCAACTGTAACTCTCTCTCCCTCTCTCAGTAATTATGAATTTTAACCCCAAGTAAGATTAGTTGAACTCCTCTGTTTCTTGCTGCAGATGTGATTGCTTACACGGCTTACATACCATCTTTTGCTGACACATGGGGATGGGTTATGGTATGAGATATTTAAATTTCGCGAAGTCATGTCTGTTGTGAATGAATTTAACTGTATGTTGTTAAAAAACAGGCATCAGATCATCCTTTCTGTATAAAATCTGAGGAATTGGATAAGAGAATTGAAGATAGAATCGATGGCGAGTTGCTCTATTTAACTGGTCCTTCCATTGTGTCCTCCACCATTATCAACAAGATGGTTTCTTTAGCGTAAGTTCATCTAACTTTATCCAATATCATTCTCAACCGATCATTCATAAATGTTTCAAAACTACTATTGAAATATAAATCTGTCGTTAAATGGAAATTAGGATATGAAATTGTGTGGTGATAGCTTAGAATTTCACTCTCTTCTAGGTGTCTACCATTCTTGTTTAAGATTTCTGTAACCGCTCAAGTTCACTCCCAGCAGATATTGTTTTCTTTGGATTTTCCCTCAATGTTTTTAAAATGGGTACGCTACATAGAGGTTTCCAAACCGTTATAAAAAATGTTTTGTTCCCCTCTCTCACTAATGTAAGATCCCACGTCGGTTGGAGAGGGGAACGAAACATTGTTTATAAGGGTGTGAAAACCTTGCGTTTTAAAATCGTGAGACTGATGGTGATACGTAATAGGTCTAATCGGATAATATCTACTAATGGTAGACTTGAGCTGTTACAATCTTAGTTTCTATCGAAGATTCTAATGATTTTAAGTTGATGAGTGATTTAATGTTTACAACTTTTTTATCAGGTTGTTGAATGAAACTCATATCTACACTGAAACGACTGCAAAATTCATACATGGGCAAGGTGTTGGATTCAAGCAACAGAATGGCGGGAGTTGTCGTGGTGTATAA

mRNA sequence

ATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGCATTGATGATCGACGGGAAGATGCAGAGCGCTGAAGCCGATGAATTCGTGTATCACGAATGCTTGATTCATCCCGCACTATTATGCCATTCCAACCCGAAAACTGTATTCATAATGGGAGGTGGGGAAGGATCAGCAGCCAGAGAAGTCCTCAAACACAAATCAATAGAAAAAGTTGTCATGTGCGATATCGATCAGGATGTAGTAGATTTCTGCCGTAAATATTTGACTGTGAACCAAGAGACATTTTGTCACAAAAAACTTCATCTTGTAATAAACGACGCCAAGGCGGAATTGGAAAAGAGAAAGGAGAAATATGATATAATTATAGGAGACCTAGCAGACCCTGTTAAAGATGGGCCTTGCTACAAGCTCTACACCAAATCCTTCTACCAGGAGATCGTCAAGCCAAAGCTCCACGACAATGGCATCTTCGTAACTCAGGCAGGACCAGCCGGAATTTTCACCCACAAAGAGGTTTTTACCTCCATATATAACACAATCAAACAGACATTCAACTATGTGATTGCTTACACGGCTTACATACCATCTTTTGCTGACACATGGGGATGGGTTATGGCATCAGATCATCCTTTCTGTATAAAATCTGAGGAATTGGATAAGAGAATTGAAGATAGAATCGATGGCGAGTTGCTCTATTTAACTGGTCCTTCCATTGTGTCCTCCACCATTATCAACAAGATGGTTTCTTTAGCGTTGTTGAATGAAACTCATATCTACACTGAAACGACTGCAAAATTCATACATGGGCAAGGTGTTGGATTCAAGCAACAGAATGGCGGGAGTTGTCGTGGTGTATAA

Coding sequence (CDS)

ATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGCATTGATGATCGACGGGAAGATGCAGAGCGCTGAAGCCGATGAATTCGTGTATCACGAATGCTTGATTCATCCCGCACTATTATGCCATTCCAACCCGAAAACTGTATTCATAATGGGAGGTGGGGAAGGATCAGCAGCCAGAGAAGTCCTCAAACACAAATCAATAGAAAAAGTTGTCATGTGCGATATCGATCAGGATGTAGTAGATTTCTGCCGTAAATATTTGACTGTGAACCAAGAGACATTTTGTCACAAAAAACTTCATCTTGTAATAAACGACGCCAAGGCGGAATTGGAAAAGAGAAAGGAGAAATATGATATAATTATAGGAGACCTAGCAGACCCTGTTAAAGATGGGCCTTGCTACAAGCTCTACACCAAATCCTTCTACCAGGAGATCGTCAAGCCAAAGCTCCACGACAATGGCATCTTCGTAACTCAGGCAGGACCAGCCGGAATTTTCACCCACAAAGAGGTTTTTACCTCCATATATAACACAATCAAACAGACATTCAACTATGTGATTGCTTACACGGCTTACATACCATCTTTTGCTGACACATGGGGATGGGTTATGGCATCAGATCATCCTTTCTGTATAAAATCTGAGGAATTGGATAAGAGAATTGAAGATAGAATCGATGGCGAGTTGCTCTATTTAACTGGTCCTTCCATTGTGTCCTCCACCATTATCAACAAGATGGTTTCTTTAGCGTTGTTGAATGAAACTCATATCTACACTGAAACGACTGCAAAATTCATACATGGGCAAGGTGTTGGATTCAAGCAACAGAATGGCGGGAGTTGTCGTGGTGTATAA

Protein sequence

MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNPKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVINDAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDRIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV

Homology

[Close]

BLAST of Csor.00g307290 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 2.7e-141
Identity = 231/309 (74.76%), Postives = 272/309 (88.03%), Query Frame = 0


Query: 42  NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEAD 101
           N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGK L+IDGKMQSAE D
Sbjct: 29  NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERD 88

Query: 102 EFVYHECLIHPALLCHSNPKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRK 161
           EF+YHECLIHPALL H NPKTVFIMGGGEGSAARE+LKH +IEKVVMCDIDQ+VVDFCR+
Sbjct: 89  EFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRR 148

Query: 162 YLTVNQETFCHKKLHLVINDAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEI 221
           +LTVN + FC+KKL LVI DAKAELEKR+EK+DII+GDLADPV+ GPCY+LYTKSFYQ I
Sbjct: 149 FLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNI 208

Query: 222 VKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMA 281
           +KPKL  NGIFVTQAGPAGIFTHKEVFTSIYNT+KQ F YV AYTA++PSFADTWGWVMA
Sbjct: 209 LKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMA 268

Query: 282 SDHPFCIKSEELDKRIEDRIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKF 341
           SDH F ++ +E+D+RIE+R++GEL+YL  PS VS+  +NK +SLAL  ET +Y+E  A+F
Sbjct: 269 SDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEENARF 328

Query: 342 IHGQGVGFK 351
           IHG GV ++
Sbjct: 329 IHGHGVAYR 337

[Close]

BLAST of Csor.00g307290 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.7e-55
Identity = 117/277 (42.24%), Postives = 166/277 (59.93%), Query Frame = 0


Query: 62  ALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNPK 121
           A+  V+ +  + +Q   L++   FGKAL++DGK+QS   DEF+YHE L+HP LL  +NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 122 TVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTV-NQETFCHKKLHLVIN 181
            + I+GGGEG+  REVLKHK+I+ V M DID  V+DF +KYL   +Q  F + K  LVI 
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 182 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 241
           D    +++ KEK+D ++ DL DP+KD P   LYTK FY+E+   ++   GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 242 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASD--HPFCIKSEELDKRIE 301
                E F+ IYNTIK  F  V A   Y+P+F   WG+V ASD  +P     EE++ RI+
Sbjct: 200 --FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIK 259

Query: 302 DRIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYT 336
           +RIDG L +  G + ++   I K +   L  E  I T
Sbjct: 260 ERIDGSLRFYDGETHITMFSIPKHIREVLEKENKIST 291

[Close]

BLAST of Csor.00g307290 vs. ExPASy Swiss-Prot
Match: Q9UXE4 (Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=speE PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 9.5e-54
Identity = 117/276 (42.39%), Postives = 166/276 (60.14%), Query Frame = 0


Query: 63  LNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSN-PK 122
           +  VL +  + +Q + L++   FGK L+IDGK+QS   DE +YHE L+HP LL  +  PK
Sbjct: 21  IEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK 80

Query: 123 TVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYL-TVNQETFCHKKLHLVIN 182
           +V I+GGGEG+  REVLK+KS+EK VM DID+ V++F +KYL   +Q  F  K+ +LVI 
Sbjct: 81  SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVIT 140

Query: 183 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 242
           D    +++ KEKYD II DL DP+K+   Y LYTK FY+++ +  L++ G  VTQA    
Sbjct: 141 DGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEKL-REILNERGGIVTQATSPS 200

Query: 243 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 302
                EV+ +IYNTIK+ F    A   Y+ SF   WG+V     P  +  +E+D +I +R
Sbjct: 201 F--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSKINER 260

Query: 303 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTE 337
           IDG+L +  G S   S  + K +       T I TE
Sbjct: 261 IDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTE 293

[Close]

BLAST of Csor.00g307290 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 4.0e-52
Identity = 112/288 (38.89%), Postives = 176/288 (61.11%), Query Frame = 0


Query: 63  LNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNPKT 122
           +  V+  G + FQD  L ++K FGK L++D  +QS E DE++YHE L+HPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 123 VFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTV-NQETFCHKKLHLVIND 182
           V I+GGGEG+  REVLKH ++EK VM DID ++V+  ++++   +Q  F   +  LVI+D
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 183 AKAELEKRKEKYDIIIGDLADPV-KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 242
           A+A LE+ +E+YD++I DL DPV +D P   LYT  FY+ +VK  L+  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMI- 200

Query: 243 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASD--HPFCIKSEELDKRIE 302
           + TH  V   ++ T+++ F YV +Y  +IP F   +G+++ASD   P       ++ RI 
Sbjct: 201 LLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIR 260

Query: 303 DRIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQG 347
           +R +  L +LT P + +  ++ K +  AL  ET + T+    ++  +G
Sbjct: 261 ER-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of Csor.00g307290 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 4.0e-52
Identity = 112/288 (38.89%), Postives = 176/288 (61.11%), Query Frame = 0


Query: 63  LNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNPKT 122
           +  V+  G + FQD  L ++K FGK L++D  +QS E DE++YHE L+HPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 123 VFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTV-NQETFCHKKLHLVIND 182
           V I+GGGEG+  REVLKH ++EK VM DID ++V+  ++++   +Q  F   +  LVI+D
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 183 AKAELEKRKEKYDIIIGDLADPV-KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 242
           A+A LE+ +E+YD++I DL DPV +D P   LYT  FY+ +VK  L+  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMI- 200

Query: 243 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASD--HPFCIKSEELDKRIE 302
           + TH  V   ++ T+++ F YV +Y  +IP F   +G+++ASD   P       ++ RI 
Sbjct: 201 LLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIR 260

Query: 303 DRIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQG 347
           +R +  L +LT P + +  ++ K +  AL  ET + T+    ++  +G
Sbjct: 261 ER-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of Csor.00g307290 vs. NCBI nr
Match: KAG6598599.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 734 bits (1895), Expect = 2.07e-267
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
           MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
           KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
           IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300

Query: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360
           IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360

[Close]

BLAST of Csor.00g307290 vs. NCBI nr
Match: XP_023546524.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 730 bits (1885), Expect = 6.92e-266
Identity = 358/360 (99.44%), Postives = 358/360 (99.44%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
           MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
           KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVN ETFCHKKLHLVIN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNHETFCHKKLHLVIN 180

Query: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
           IFTHKEVFTSIYNTIK TFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR
Sbjct: 241 IFTHKEVFTSIYNTIKHTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300

Query: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360
           IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360

[Close]

BLAST of Csor.00g307290 vs. NCBI nr
Match: XP_022997196.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima])

HSP 1 Score: 729 bits (1881), Expect = 2.82e-265
Identity = 356/360 (98.89%), Postives = 359/360 (99.72%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
           MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVIN VNGNDCYWFEEIIDENLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
           KTVFIMGGGEGSAAREVLKH+SIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHRSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
           IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSE+LDK+IEDR
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEDLDKKIEDR 300

Query: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360
           IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360

[Close]

BLAST of Csor.00g307290 vs. NCBI nr
Match: XP_022961616.1 (thermospermine synthase ACAULIS5 [Cucurbita moschata])

HSP 1 Score: 728 bits (1879), Expect = 5.69e-265
Identity = 357/360 (99.17%), Postives = 358/360 (99.44%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
           MGPAVLSSHTNGFSKSVDRNFEEK+NYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
           KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
           IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300

Query: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360
           IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTE TAKFIHGQGVGFKQQNG SCRGV
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTEATAKFIHGQGVGFKQQNGRSCRGV 360

[Close]

BLAST of Csor.00g307290 vs. NCBI nr
Match: XP_038886733.1 (thermospermine synthase ACAULIS5 isoform X1 [Benincasa hispida])

HSP 1 Score: 686 bits (1769), Expect = 3.59e-248
Identity = 339/362 (93.65%), Postives = 351/362 (96.96%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYING-DSSHESVINTVNGNDCYWFEEIIDENLKW 60
           MGPAVLSSHTNGFS+SVDRNFEEKSNYING +SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1   MGPAVLSSHTNGFSQSVDRNFEEKSNYINGANSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61  SFALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSN 120
           SFALN VLQKGTSEFQDIALLDTK FGK LMIDGKMQSAEADEFVYHECLIHPALLCHSN
Sbjct: 61  SFALNRVLQKGTSEFQDIALLDTKRFGKTLMIDGKMQSAEADEFVYHECLIHPALLCHSN 120

Query: 121 PKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVI 180
           PKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRK+LTVN+ETFCH KLHLVI
Sbjct: 121 PKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKHLTVNRETFCHSKLHLVI 180

Query: 181 NDAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPA 240
           NDA+AELEKR+EKYDIIIGDLADPVKDGPCYKLYTKSFY +IVKPKLH NGIFVTQAGPA
Sbjct: 181 NDARAELEKRREKYDIIIGDLADPVKDGPCYKLYTKSFYHDIVKPKLHHNGIFVTQAGPA 240

Query: 241 GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED 300
           GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED
Sbjct: 241 GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED 300

Query: 301 RIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSC-R 360
           RI+GELLYLTGPSIVSSTI+NKMVSLALLNETHIYTE TAKFIHGQGVGFKQ NGGSC R
Sbjct: 301 RINGELLYLTGPSIVSSTILNKMVSLALLNETHIYTEATAKFIHGQGVGFKQLNGGSCGR 360

[Close]

BLAST of Csor.00g307290 vs. ExPASy TrEMBL
Match: A0A6J1K6U5 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111492169 PE=3 SV=1)

HSP 1 Score: 729 bits (1881), Expect = 1.36e-265
Identity = 356/360 (98.89%), Postives = 359/360 (99.72%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
           MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVIN VNGNDCYWFEEIIDENLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
           KTVFIMGGGEGSAAREVLKH+SIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHRSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
           IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSE+LDK+IEDR
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEDLDKKIEDR 300

Query: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360
           IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360

[Close]

BLAST of Csor.00g307290 vs. ExPASy TrEMBL
Match: A0A6J1HAV2 (thermospermine synthase ACAULIS5 OS=Cucurbita moschata OX=3662 GN=LOC111462336 PE=3 SV=1)

HSP 1 Score: 728 bits (1879), Expect = 2.75e-265
Identity = 357/360 (99.17%), Postives = 358/360 (99.44%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
           MGPAVLSSHTNGFSKSVDRNFEEK+NYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
           KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
           IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300

Query: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCRGV 360
           IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTE TAKFIHGQGVGFKQQNG SCRGV
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTEATAKFIHGQGVGFKQQNGRSCRGV 360

[Close]

BLAST of Csor.00g307290 vs. ExPASy TrEMBL
Match: A0A1S3BCH1 (thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV=1)

HSP 1 Score: 670 bits (1728), Expect = 3.20e-242
Identity = 333/363 (91.74%), Postives = 348/363 (95.87%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
           MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1   MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61  SFALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSN 120
           SFALN VLQ+GTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHECLIHP LLCHSN
Sbjct: 61  SFALNRVLQQGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHECLIHPPLLCHSN 120

Query: 121 PKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVI 180
           PK+VFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRK+LTVN+E FCHKKLHLVI
Sbjct: 121 PKSVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKHLTVNREAFCHKKLHLVI 180

Query: 181 NDAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPA 240
           NDA+ ELEKRKEKYD+IIGDLADPVKDGPCYKLYTKSFY +I+KPKLH NGIFVTQAGPA
Sbjct: 181 NDARVELEKRKEKYDVIIGDLADPVKDGPCYKLYTKSFYHDILKPKLHHNGIFVTQAGPA 240

Query: 241 GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED 300
           GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED
Sbjct: 241 GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED 300

Query: 301 RIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGV-GFKQQNGGSC- 360
           RI+GELLYLTGPSIVSSTIINK+VSLALLNETHIYTE TAKFIHGQGV G KQ NGGSC 
Sbjct: 301 RINGELLYLTGPSIVSSTIINKIVSLALLNETHIYTEATAKFIHGQGVVGCKQLNGGSCG 360

[Close]

BLAST of Csor.00g307290 vs. ExPASy TrEMBL
Match: A0A6J1BPF9 (thermospermine synthase ACAULIS5 OS=Momordica charantia OX=3673 GN=LOC111004633 PE=3 SV=1)

HSP 1 Score: 648 bits (1671), Expect = 1.03e-233
Identity = 319/358 (89.11%), Postives = 338/358 (94.41%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
           MGPAVLSSHTNGFSK     F++KSNYING+SSHE+VI+  NG+DC+WFEEIID+NLKWS
Sbjct: 1   MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120

Query: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
           K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180

Query: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
           IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300

Query: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358
           IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351

[Close]

BLAST of Csor.00g307290 vs. ExPASy TrEMBL
Match: A0A0A0KIK6 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1)

HSP 1 Score: 636 bits (1641), Expect = 3.30e-229
Identity = 321/363 (88.43%), Postives = 334/363 (92.01%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
           MGPAVLSSHTN               Y NGD SSHESVIN V+G+DC+W+EEIIDENLKW
Sbjct: 1   MGPAVLSSHTN---------------YTNGDNSSHESVINNVSGDDCHWYEEIIDENLKW 60

Query: 61  SFALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSN 120
           SFALN VLQ+GTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHECLIHPALLCHSN
Sbjct: 61  SFALNRVLQQGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSN 120

Query: 121 PKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVI 180
           PK+VFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRK+LTVN+E FCHKKLHLVI
Sbjct: 121 PKSVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKHLTVNREAFCHKKLHLVI 180

Query: 181 NDAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPA 240
           NDA+ ELEKRKEKYD+IIGDLADPVKDGPCYKLYTKSFY +IVKPKLH NGIFVTQAGPA
Sbjct: 181 NDARVELEKRKEKYDVIIGDLADPVKDGPCYKLYTKSFYHDIVKPKLHHNGIFVTQAGPA 240

Query: 241 GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED 300
           GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED
Sbjct: 241 GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED 300

Query: 301 RIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGV-GFKQQNGGSC- 360
           RI+GELLYLTGPSIVSSTIINK+VSLALLNETHIYTE TAKFIHGQGV G KQ NGGSC 
Sbjct: 301 RINGELLYLTGPSIVSSTIINKIVSLALLNETHIYTEATAKFIHGQGVVGCKQLNGGSCG 348

[Close]

BLAST of Csor.00g307290 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 503.1 bits (1294), Expect = 1.9e-142
Identity = 231/309 (74.76%), Postives = 272/309 (88.03%), Query Frame = 0


Query: 42  NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEAD 101
           N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGK L+IDGKMQSAE D
Sbjct: 29  NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERD 88

Query: 102 EFVYHECLIHPALLCHSNPKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRK 161
           EF+YHECLIHPALL H NPKTVFIMGGGEGSAARE+LKH +IEKVVMCDIDQ+VVDFCR+
Sbjct: 89  EFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRR 148

Query: 162 YLTVNQETFCHKKLHLVINDAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEI 221
           +LTVN + FC+KKL LVI DAKAELEKR+EK+DII+GDLADPV+ GPCY+LYTKSFYQ I
Sbjct: 149 FLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNI 208

Query: 222 VKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMA 281
           +KPKL  NGIFVTQAGPAGIFTHKEVFTSIYNT+KQ F YV AYTA++PSFADTWGWVMA
Sbjct: 209 LKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMA 268

Query: 282 SDHPFCIKSEELDKRIEDRIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKF 341
           SDH F ++ +E+D+RIE+R++GEL+YL  PS VS+  +NK +SLAL  ET +Y+E  A+F
Sbjct: 269 SDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEENARF 328

Query: 342 IHGQGVGFK 351
           IHG GV ++
Sbjct: 329 IHGHGVAYR 337

[Close]

BLAST of Csor.00g307290 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 503.1 bits (1294), Expect = 1.9e-142
Identity = 231/309 (74.76%), Postives = 272/309 (88.03%), Query Frame = 0


Query: 42  NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEAD 101
           N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGK L+IDGKMQSAE D
Sbjct: 22  NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERD 81

Query: 102 EFVYHECLIHPALLCHSNPKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRK 161
           EF+YHECLIHPALL H NPKTVFIMGGGEGSAARE+LKH +IEKVVMCDIDQ+VVDFCR+
Sbjct: 82  EFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRR 141

Query: 162 YLTVNQETFCHKKLHLVINDAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEI 221
           +LTVN + FC+KKL LVI DAKAELEKR+EK+DII+GDLADPV+ GPCY+LYTKSFYQ I
Sbjct: 142 FLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNI 201

Query: 222 VKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMA 281
           +KPKL  NGIFVTQAGPAGIFTHKEVFTSIYNT+KQ F YV AYTA++PSFADTWGWVMA
Sbjct: 202 LKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMA 261

Query: 282 SDHPFCIKSEELDKRIEDRIDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKF 341
           SDH F ++ +E+D+RIE+R++GEL+YL  PS VS+  +NK +SLAL  ET +Y+E  A+F
Sbjct: 262 SDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVYSEENARF 321

Query: 342 IHGQGVGFK 351
           IHG GV ++
Sbjct: 322 IHGHGVAYR 330

[Close]

BLAST of Csor.00g307290 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 137.9 bits (346), Expect = 1.6e-32
Identity = 85/262 (32.44%), Postives = 138/262 (52.67%), Query Frame = 0


Query: 29  NGDSSHE-SVINTVNGNDCYWFEEIIDENLKW-----SFALNSVLQKGTSEFQDIALLDT 88
           NGD   E +  +TV      WF E+   +  W     S  +  VL +G S++QD+ +  +
Sbjct: 74  NGDQKKEPACFSTVIPG---WFSEM---SPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQS 133

Query: 89  KCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNPKTVFIMGGGEGSAAREVLKHKS 148
             +GK L++DG +Q  E DE  Y E + H  L    NPK V ++GGG+G   REV +H S
Sbjct: 134 ATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHAS 193

Query: 149 IEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVINDAKAELEKRKE-KYDIIIGDLA 208
           IE++ MC+ID+ VVD  +++       +   +++LVI D  A L+   E  YD +I D +
Sbjct: 194 IEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSS 253

Query: 209 DPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFNY 268
           DP+  GP  +L+ K F+Q + +  L   G+  TQA    ++ H ++   I +  ++ F  
Sbjct: 254 DPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQA--ESLWLHMDIIEDIVSNCREIFKG 313

Query: 269 VIAYT-AYIPSFADTWG-WVMA 282
            + Y    +P++   WG W+ A
Sbjct: 314 SVNYAWTSVPTYPRHWGHWIYA 324

[Close]

BLAST of Csor.00g307290 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 132.5 bits (332), Expect = 6.8e-31
Identity = 74/224 (33.04%), Postives = 120/224 (53.57%), Query Frame = 0


Query: 48  WFEEIIDENLKW-----SFALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADE 107
           WF EI   +  W     S  +  +L +G S++QD+ +  +  +GK L++DG +Q  E DE
Sbjct: 51  WFSEI---SPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDE 110

Query: 108 FVYHECLIHPALLCHSNPKTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKY 167
             Y E + H  L   SNPK V ++GGG+G   REV +H S+E++ +C+ID+ VVD  ++Y
Sbjct: 111 CAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQY 170

Query: 168 LTVNQETFCHKKLHLVINDAKAELEKRKE-KYDIIIGDLADPVKDGPCYKLYTKSFYQEI 227
                  +   +++L+I D  A L+   E  YD +I D +DP+  GP  +L+ K F+ E 
Sbjct: 171 FPNVAVGYEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFF-ES 230

Query: 228 VKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFNYVIAY 266
           V   L   G+  TQA    ++ H ++   I +  +  F   + Y
Sbjct: 231 VNRALRPGGVVCTQA--ESLWLHMDIIEDIVSNCRDIFKGSVNY 266

[Close]

BLAST of Csor.00g307290 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 131.3 bits (329), Expect = 1.5e-30
Identity = 80/244 (32.79%), Postives = 129/244 (52.87%), Query Frame = 0


Query: 29  NGDSSHE-SVINTVNGNDCYWFEEIIDENLKW-----SFALNSVLQKGTSEFQDIALLDT 88
           NGD   E +  +TV      WF E+   +  W     S  +  VL +G S++QD+ +  +
Sbjct: 74  NGDQKKEPACFSTVIPG---WFSEM---SPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQS 133

Query: 89  KCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNPKTVFIMGGGEGSAAREVLKHKS 148
             +GK L++DG +Q  E DE  Y E + H  L    NPK V ++GGG+G   REV +H S
Sbjct: 134 ATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHAS 193

Query: 149 IEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVINDAKAELEKRKE-KYDIIIGDLA 208
           IE++ MC+ID+ VVD  +++       +   +++LVI D  A L+   E  YD +I D +
Sbjct: 194 IEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSS 253

Query: 209 DPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFNY 266
           DP+  GP  +L+ K F+Q + +  L   G+  TQA    ++ H ++   I +  ++ F  
Sbjct: 254 DPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQA--ESLWLHMDIIEDIVSNCREIFKG 306

The following BLAST results are available for this feature:

BLAST of Csor.00g307290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7X6	2.7e-141	74.76	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]
Q975S5	1.7e-55	42.24	Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ...	[more]
Q9UXE4	9.5e-54	42.39	Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 3509...	[more]
Q72K55	4.0e-52	38.89	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ...	[more]
Q5SK28	4.0e-52	38.89	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS...	[more]

BLAST of Csor.00g307290 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6598599.1	2.07e-267	100.00	Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_023546524.1	6.92e-266	99.44	thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997196.1	2.82e-265	98.89	thermospermine synthase ACAULIS5-like [Cucurbita maxima]	[more]
XP_022961616.1	5.69e-265	99.17	thermospermine synthase ACAULIS5 [Cucurbita moschata]	[more]
XP_038886733.1	3.59e-248	93.65	thermospermine synthase ACAULIS5 isoform X1 [Benincasa hispida]	[more]

BLAST of Csor.00g307290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K6U5	1.36e-265	98.89	thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149216...	[more]
A0A6J1HAV2	2.75e-265	99.17	thermospermine synthase ACAULIS5 OS=Cucurbita moschata OX=3662 GN=LOC111462336 P...	[more]
A0A1S3BCH1	3.20e-242	91.74	thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV...	[more]
A0A6J1BPF9	1.03e-233	89.11	thermospermine synthase ACAULIS5 OS=Momordica charantia OX=3673 GN=LOC111004633 ...	[more]
A0A0A0KIK6	3.30e-229	88.43	PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 S...	[more]

BLAST of Csor.00g307290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19530.1	1.9e-142	74.76	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G19530.2	1.9e-142	74.76	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G23820.2	1.6e-32	32.44	spermidine synthase 1	[more]
AT1G70310.1	6.8e-31	33.04	spermidine synthase 2	[more]
AT1G23820.1	1.5e-30	32.79	spermidine synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 99..351 e-value: 1.6E-69 score: 236.2
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 102..282 e-value: 4.0E-42 score: 143.8
None	No IPR available	PANTHER	PTHR43317	THERMOSPERMINE SYNTHASE ACAULIS5	coord: 38..350
None	No IPR available	PANTHER	PTHR43317:SF8	BNAA02G04280D PROTEIN	coord: 38..350
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 127..235 e-value: 1.40273E-6 score: 44.3431
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 47..99 e-value: 3.8E-12 score: 45.9
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 47..98 e-value: 1.9E-13 score: 52.2
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 47..319 score: 29.870644
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 46..283 score: 92.97934
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 46..316

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g307290.m01	Csor.00g307290.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0010487	thermospermine synthase activity
molecular_function	GO:0003824	catalytic activity