Csor.00g306860 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g306860
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	cytochrome b5
Location	Csor_Chr05: 2220757 .. 2222372 (+)
RNA-Seq Expression	Csor.00g306860
Synteny	Csor.00g306860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGAGCATTTCGGCCCTCTATTCCATAGAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTACGTCCCTGATAGTATTCATGGCCTTACTTAATTTTCTTTCACAAATTTTTGTTCCGTTCGTCGGCCTTACAGTATTCTCTTCATGTATGTGCTTCGCCATATGATTCTCATGGAATTTTTTTCTGAGTTTTTTTTTTTTTTTTTTTTTTTCGATTTGTTATTCCACGAGATGCGTTCTAAATTATGGTCGGAATTTGTTTAAGGCGCTCTCGAGTTGTGGACTGAATTCAATTTTTGTGGTTGATTGTCGTCTTTTTGGCTGGGGGATGGGAAGTTGATGCTATAGTTTTACTGGAATTAGCTGAACTTTGTCACTGTGATGGTGAGAAATGAAGTAAACAATCTTATCATGTTCAGATTTTTTTATTTCTGATATTCCATTATACAGTGTTCATAGTTGTAATTGATGAGGATCGAGACTCTTTAAGTTTAAATGAAGTTCTGTTTAGAAGTAACTGTCTGATTAATATAGGCATGAATTTATAAACCATTTTAGGAGTTGAGATCTGATGAAATCTCTTGGCCTTAATTTCTCCTATCCACCTGAAGTTTCTTCTTCTACTATCTAACTTTGTTTTGTTCCCACTCTTGGAGGCAGAGTGGAGATAACCGGATTAAAGTAGAAGGTAAAAGAATGGTGGATGGATGGATGGATGGAAACTCACCAACATGGTTCACTCTTAAACCAATAGATTTATTTTGATTTCTAGTTCATCGTCATGTCAAATTCTTAGAATTAACAATACAAAAGTAAGGGATTCCAATACTGAATTCAAATAGGACTCGAAGTTTCAATATCCTCTTAGAACGTATGTTACTGTTTGAATCTGATACTTTATTGGATTGGATGTGTCAATTCTGACAAATTGTTGATTCAAAATTCTTGAGAGGGAAATTGACGTTTTAGTTGCGTGTTTGAAACATCGGCCCTCCATTTCCTCGCTCACCATCTTTGCCAAAATATTGATACTTTCGTTTTTACTTCGGTCCACGATCAATTTTCTGTCCACACATTCTCCATTGACACCATTCTACAACATGTCCACCTCCTCTGACCAGTAAAAATGTGTCATCTTTTACCATATTTTGTTTGAGAGGATGCGTTCTGACATTACTTCAAAATGACATGTTATCGTTCATAGGTGTATGATTTGACGTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGTATGGTATGCTTGTACTTTGTAATGGATTCTTCTCTCACAAATCTGAACACTGCACTTTTTTGTCCCATCAAAGGCAGAGATGCCACAGATGATTTCGAAGATGCCGGTCATAGCAAAGATGCTCGAGAGCTGATGGAGAAGTTCTATATCGGCGAGCTTGATCCTTCTTCCTCAGCGATCCCAGAACTCGAAACAAAAGAACCAGATGGTTATGCAGCTCGGGTTCAGACCTTGACAAAGCAATACTGGGGGGTTCCTATTGCAGTACTTGGTATTTCTGTAGTACTTGGCTGGTTATACTTGCTCAAAAAGTGA

mRNA sequence

ATGCCGAGCATTTCGGCCCTCTATTCCATAGAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTGTATGATTTGACGTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGCAGAGATGCCACAGATGATTTCGAAGATGCCGGTCATAGCAAAGATGCTCGAGAGCTGATGGAGAAGTTCTATATCGGCGAGCTTGATCCTTCTTCCTCAGCGATCCCAGAACTCGAAACAAAAGAACCAGATGGTTATGCAGCTCGGGTTCAGACCTTGACAAAGCAATACTGGGGGGTTCCTATTGCAGTACTTGGTATTTCTGTAGTACTTGGCTGGTTATACTTGCTCAAAAAGTGA

Coding sequence (CDS)

ATGCCGAGCATTTCGGCCCTCTATTCCATAGAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTGTATGATTTGACGTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGCAGAGATGCCACAGATGATTTCGAAGATGCCGGTCATAGCAAAGATGCTCGAGAGCTGATGGAGAAGTTCTATATCGGCGAGCTTGATCCTTCTTCCTCAGCGATCCCAGAACTCGAAACAAAAGAACCAGATGGTTATGCAGCTCGGGTTCAGACCTTGACAAAGCAATACTGGGGGGTTCCTATTGCAGTACTTGGTATTTCTGTAGTACTTGGCTGGTTATACTTGCTCAAAAAGTGA

Protein sequence

MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK

Homology

[Close]

BLAST of Csor.00g306860 vs. ExPASy Swiss-Prot
Match: Q9FDW8 (Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 3.7e-40
Identity = 78/134 (58.21%), Postives = 104/134 (77.61%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LYS+EE + H+  DDCW++IDGKVYD++SY+DEHPGGDDV++A  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSS-SAIPEL---ETKEPDGYAARVQTLTKQYWGVPIAV 120
           DAGHSKDARELMEK++IGELD SS   IPEL   +  +P     ++  LTKQYW VP+++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LGISVVLGWLYLLK 131
           + ISV +  L+  K
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of Csor.00g306860 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 7.3e-28
Identity = 56/121 (46.28%), Postives = 90/121 (74.38%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDDV++++TG+DATDDFED GHS+
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 67  DARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQY------WGVPIAVLGIS 122
            ARE+ME++Y+GE+DP++  IP+     P       Q  T ++      + VP+A+LG++
Sbjct: 67  SAREMMEQYYVGEIDPTT--IPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLA 125

[Close]

BLAST of Csor.00g306860 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.2e-27
Identity = 61/129 (47.29%), Postives = 88/129 (68.22%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           M S   +   EEVSQH+   DCW+II GKVYD+T ++D+HPGGD+V++++TG+DAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQY------WGVPI 120
           D GHS  AR++MEK+YIGE+D  SS +P   T       A  Q  T ++      + VPI
Sbjct: 61  DVGHSDTARDMMEKYYIGEID--SSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPI 120

Query: 121 AVLGISVVL 124
            +LG+++V+
Sbjct: 121 LILGLALVV 127

[Close]

BLAST of Csor.00g306860 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 6.1e-27
Identity = 59/129 (45.74%), Postives = 90/129 (69.77%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           M S   + S EEVS+H+   DCW+II GKVYD+T ++D+HPGGD+V++++TG+DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQY------WGVPI 120
           D GHS  AR++M+K++IGE+D  SS++P   T       A  Q  T ++      + VPI
Sbjct: 61  DVGHSDTARDMMDKYFIGEID--SSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPI 120

Query: 121 AVLGISVVL 124
            +LG+++V+
Sbjct: 121 LILGLALVV 127

[Close]

BLAST of Csor.00g306860 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 1.4e-26
Identity = 55/123 (44.72%), Postives = 89/123 (72.36%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           +Y++EEV++H+S DDCW+II GKVY+++ +L++HPGGDDV++++TG+DATDDFED GHS 
Sbjct: 8   VYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHST 67

Query: 67  DARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQY------WGVPIAVLGIS 124
            AR +M+++Y+G++D  +S IP      P       Q  T ++      + VP+A+LG++
Sbjct: 68  TARAMMDEYYVGDID--TSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLA 127

[Close]

BLAST of Csor.00g306860 vs. NCBI nr
Match: KAG6598642.1 (Cytochrome b5 isoform A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 264 bits (674), Expect = 6.74e-89
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120

Query: 121 VVLGWLYLLKK 131
           VVLGWLYLLKK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of Csor.00g306860 vs. NCBI nr
Match: XP_022961879.1 (cytochrome b5 [Cucurbita moschata])

HSP 1 Score: 261 bits (668), Expect = 5.55e-88
Identity = 129/131 (98.47%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKDARELMEKFYIGELDPSSSAIPELE KEPDGYAARVQTLTKQYWG+PIAVLGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELEAKEPDGYAARVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVLGWLYLLKK 131
           VVLGWLYLLKK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of Csor.00g306860 vs. NCBI nr
Match: XP_023545611.1 (cytochrome b5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261 bits (667), Expect = 7.88e-88
Identity = 129/131 (98.47%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLG+S
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGMS 120

Query: 121 VVLGWLYLLKK 131
           V LGWLYLLKK
Sbjct: 121 VALGWLYLLKK 131

[Close]

BLAST of Csor.00g306860 vs. NCBI nr
Match: XP_022997294.1 (cytochrome b5 [Cucurbita maxima])

HSP 1 Score: 254 bits (649), Expect = 4.39e-85
Identity = 123/131 (93.89%), Postives = 129/131 (98.47%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIEE+S+HSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEISRHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIIAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKD RELM+KFYIGELDPSSSAIP LETKEPDGYAA+VQTLTKQYWG+PIAVLGIS
Sbjct: 61  DAGHSKDTRELMKKFYIGELDPSSSAIPALETKEPDGYAAQVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVLGWLYLLKK 131
           VVLGWLYLLKK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of Csor.00g306860 vs. NCBI nr
Match: KAG7029579.1 (Cytochrome b5 isoform A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250 bits (638), Expect = 4.84e-83
Identity = 131/156 (83.97%), Postives = 131/156 (83.97%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATG-------- 60
           MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATG        
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60

Query: 61  -----------------RDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                            RDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP
Sbjct: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120

Query: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 131
           DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK
Sbjct: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 156

[Close]

BLAST of Csor.00g306860 vs. ExPASy TrEMBL
Match: A0A6J1HFA7 (cytochrome b5 OS=Cucurbita moschata OX=3662 GN=LOC111462520 PE=3 SV=1)

HSP 1 Score: 261 bits (668), Expect = 2.69e-88
Identity = 129/131 (98.47%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKDARELMEKFYIGELDPSSSAIPELE KEPDGYAARVQTLTKQYWG+PIAVLGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELEAKEPDGYAARVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVLGWLYLLKK 131
           VVLGWLYLLKK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of Csor.00g306860 vs. ExPASy TrEMBL
Match: A0A6J1KB15 (cytochrome b5 OS=Cucurbita maxima OX=3661 GN=LOC111492240 PE=3 SV=1)

HSP 1 Score: 254 bits (649), Expect = 2.13e-85
Identity = 123/131 (93.89%), Postives = 129/131 (98.47%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIEE+S+HSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEISRHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIIAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKD RELM+KFYIGELDPSSSAIP LETKEPDGYAA+VQTLTKQYWG+PIAVLGIS
Sbjct: 61  DAGHSKDTRELMKKFYIGELDPSSSAIPALETKEPDGYAAQVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVLGWLYLLKK 131
           VVLGWLYLLKK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of Csor.00g306860 vs. ExPASy TrEMBL
Match: A0A0A0KIR1 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G366540 PE=3 SV=1)

HSP 1 Score: 226 bits (576), Expect = 2.89e-74
Identity = 113/131 (86.26%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSI+EVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKDARELMEKFYIG LD SS    +LET + DGYA RVQTLTKQYW  P+AV+GIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWLYLLKK 131
           VVLGW YL KK
Sbjct: 121 VVLGWAYLRKK 131

[Close]

BLAST of Csor.00g306860 vs. ExPASy TrEMBL
Match: A0A6J1BQT8 (cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111004609 PE=3 SV=1)

HSP 1 Score: 225 bits (573), Expect = 8.27e-74
Identity = 108/131 (82.44%), Postives = 120/131 (91.60%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSIS LYSI+E+S HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKDARELM+KFYIGELD SSSAIPEL+T++ D YAARVQ L KQYW  P+A+LGIS
Sbjct: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120

Query: 121 VVLGWLYLLKK 131
           VV+GWL+L  K
Sbjct: 121 VVIGWLFLRNK 131

[Close]

BLAST of Csor.00g306860 vs. ExPASy TrEMBL
Match: A0A1S3BCR4 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1)

HSP 1 Score: 222 bits (565), Expect = 1.37e-72
Identity = 109/131 (83.21%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSI+EVSQHSS+DDCWIIIDGKVYDLTSYLDEHPGGDD+IV ATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120
           DAGHSKDARELMEKFYIG LD SSS   +LET + D YA RVQTLTKQYW  P+AV+GIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQVDSYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWLYLLKK 131
           +VLGW+YL KK
Sbjct: 121 MVLGWVYLRKK 131

[Close]

BLAST of Csor.00g306860 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 165.6 bits (418), Expect = 2.6e-41
Identity = 78/134 (58.21%), Postives = 104/134 (77.61%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LYS+EE + H+  DDCW++IDGKVYD++SY+DEHPGGDDV++A  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSS-SAIPEL---ETKEPDGYAARVQTLTKQYWGVPIAV 120
           DAGHSKDARELMEK++IGELD SS   IPEL   +  +P     ++  LTKQYW VP+++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LGISVVLGWLYLLK 131
           + ISV +  L+  K
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of Csor.00g306860 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 124.8 bits (312), Expect = 5.2e-29
Identity = 56/121 (46.28%), Postives = 90/121 (74.38%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDDV++++TG+DATDDFED GHS+
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 67  DARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQY------WGVPIAVLGIS 122
            ARE+ME++Y+GE+DP++  IP+     P       Q  T ++      + VP+A+LG++
Sbjct: 67  SAREMMEQYYVGEIDPTT--IPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLA 125

[Close]

BLAST of Csor.00g306860 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 121.7 bits (304), Expect = 4.4e-28
Identity = 59/129 (45.74%), Postives = 90/129 (69.77%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           M S   + S EEVS+H+   DCW+II GKVYD+T ++D+HPGGD+V++++TG+DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQY------WGVPI 120
           D GHS  AR++M+K++IGE+D  SS++P   T       A  Q  T ++      + VPI
Sbjct: 61  DVGHSDTARDMMDKYFIGEID--SSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPI 120

Query: 121 AVLGISVVL 124
            +LG+++V+
Sbjct: 121 LILGLALVV 127

[Close]

BLAST of Csor.00g306860 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 117.9 bits (294), Expect = 6.3e-27
Identity = 55/126 (43.65%), Postives = 84/126 (66.67%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           ++++ EVSQHSS  DCWI+IDGKVYD+T +LD+HPGGD+VI+ +TG+DATDDFED GHS 
Sbjct: 7   VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 67  DARELMEKFYIGELD------------PSSSAIPELETKEPDGYAARVQTLTKQYWGVPI 121
            A+ +++++Y+G++D            P+S+     + K  D     +Q L      VP+
Sbjct: 67  TAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFL------VPL 126

[Close]

BLAST of Csor.00g306860 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 109.8 bits (273), Expect = 1.7e-24
Identity = 52/124 (41.94%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           L S  +V++H   +DCWI+I GKVYD+++++DEHPGGD+V++A TG+DA+ DFED  HSK
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  DARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWG-------VPIAVLGI 124
           DA+ELM+K+ IG++D S+  + +      +  +   +T TK+  G       +P+ +LG+
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAET-TKEESGKKLLIYLIPLLILGV 123

The following BLAST results are available for this feature:

BLAST of Csor.00g306860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FDW8	3.7e-40	58.21	Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1	[more]
O48845	7.3e-28	46.28	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P40934	1.2e-27	47.29	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
Q42342	6.1e-27	45.74	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P49100	1.4e-26	44.72	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of Csor.00g306860 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6598642.1	6.74e-89	100.00	Cytochrome b5 isoform A, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022961879.1	5.55e-88	98.47	cytochrome b5 [Cucurbita moschata]	[more]
XP_023545611.1	7.88e-88	98.47	cytochrome b5 [Cucurbita pepo subsp. pepo]	[more]
XP_022997294.1	4.39e-85	93.89	cytochrome b5 [Cucurbita maxima]	[more]
KAG7029579.1	4.84e-83	83.97	Cytochrome b5 isoform A [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Csor.00g306860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HFA7	2.69e-88	98.47	cytochrome b5 OS=Cucurbita moschata OX=3662 GN=LOC111462520 PE=3 SV=1	[more]
A0A6J1KB15	2.13e-85	93.89	cytochrome b5 OS=Cucurbita maxima OX=3661 GN=LOC111492240 PE=3 SV=1	[more]
A0A0A0KIR1	2.89e-74	86.26	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1BQT8	8.27e-74	82.44	cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111004609 PE=3 SV=1	[more]
A0A1S3BCR4	1.37e-72	83.21	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1	[more]

BLAST of Csor.00g306860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26340.1	2.6e-41	58.21	cytochrome B5 isoform A	[more]
AT2G32720.1	5.2e-29	46.28	cytochrome B5 isoform B	[more]
AT5G53560.1	4.4e-28	45.74	cytochrome B5 isoform E	[more]
AT5G48810.1	6.3e-27	43.65	cytochrome B5 isoform D	[more]
AT2G46650.1	1.7e-24	41.94	cytochrome B5 isoform C	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 40..54 score: 51.92 coord: 68..80 score: 48.25 coord: 30..40 score: 55.65 coord: 55..62 score: 44.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 8.8E-33 score: 124.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 10..80 e-value: 3.3E-26 score: 91.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 29.246239
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 5..90 e-value: 1.1E-34 score: 120.3
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..85
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 3..129
None	No IPR available	PANTHER	PTHR19359:SF25	CYTOCHROME B5 ISOFORM A	coord: 3..129
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g306860.m01	Csor.00g306860.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009707	chloroplast outer membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0010319	stromule
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding