Csor.00g306610 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g306610
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioneukaryotic translation initiation factor 5-like
LocationCsor_Chr05: 2427041 .. 2428387 (-)
RNA-Seq ExpressionCsor.00g306610
SyntenyCsor.00g306610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAGAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAATGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAACTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

mRNA sequence

ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAGAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAATGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAACTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

Coding sequence (CDS)

ATGGCGTTACAGAACATTGGTGCCTCAAACCGTGACGATGCCTTCTATAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGACATTGCAAAAGCCTTGGGCAGACCTGCTGCTTACACTACCAAGTACTTCGGATGTGAGCTTGGCGCACAATCCAAGTTTGATGAGAAAACAGGTATTGCTCTTGTAAATGGAGCACATGAGACGGCGAAGTTGGCTGGTCTTCTCGAGAACTTCATCAAGAAATATGTTCAATGCTATGGTTGTGGAAACCCTGAAACTGAGATAATCATCACTAAGACGCAGATGATCAATATGAAATGTGCTGCTTGTGGATTCATATCTGATGTTGATATGAGAGATAAACTCACTACATTTATTCTGAAGAATCCTCCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGCGGGCTGAGAAGGAACGGCTTAAGGAAGGAGAGGCTGCTGATGAGGAACTGAAGAAAATAAAGAAGGATTCCCTGAAGAAGAAGAGCACTAGTGGTAATGCAAAGGAAGCTGTAACCAAAGTTCCAAGCAAGAAGAAGAGTAACGGCTCTGATGAGGATCATTCTCCAACTCGTAGCCATGCCGTTGAGAATGACCAAGCTGCTGACGACGACGACGACGACGATGTACAGTGGCAAACAGACACATCTTTGGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCTGATATGGTCATGCTCTCTACAACTGAAGAGACAAAGTCGTCAAAGAAGTCCCCCGAACACGAGAAGTCTGTGGTCAATGGAAGCAAGAATGGAAATGGAGTGTCTACCCATGGTGCCCTTGTTCATGAGATCAAAGATTATTTGAAGAAAACTCCCTCTGCAGCCGATCTTAAATCATTCTTGGAGTCGCTTACTGGAACTCACCAAGAGACTGCGAATGCCTTAGTTGAGGCTCTATTTGAAGGAGTTGGTAAAGGGTTCTCAAAGGAGGTAGTGAAAAAGAAGAAGTTCCTGGTTGCAGTTGCAGCAGCTCAGGAGGAGGGATCACAGATAGTTCTGTTGCGTTCCATCGAATCTTTCTGTCTCAACACGAGTCCGGAGGCGGCTAAGGAGATCGCACTGGTCCTGAAATCGTTATACGATGGGGATGTAATAGAGGAGGAATTCGTGCTAGAGTGGTACCAGCAGGGACTGGCAGGAGCTAACAAAGGTAGCCAAATCTGGAAGCATGTAAAGCCGTTCATCGAGTGGCTCCAGAACGCAGAGTCGGAGACGGAAGAAGAGTAA

Protein sequence

MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMINMKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKIKKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGALVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVAVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE
Homology
BLAST of Csor.00g306610 vs. ExPASy Swiss-Prot
Match: P48724 (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 7.9e-159
Identity = 312/454 (68.72%), Postives = 361/454 (79.52%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGA N DDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAK L RPA+YTTKYFG
Sbjct: 1   MALQNIGAGNSDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKRLARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG + VNGAHETAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QMI 
Sbjct: 61  CELGAQSKFDEKTGTSHVNGAHETAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMIQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGF+SDVDMRDKLTTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEELKK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIVKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKV 180

Query: 181 KKDSLKKKSTS---GNAKEAVTKVPSKKKSNGSDED-HSPTRSHAVENDQAADDDDDDD- 240
           KK+  KK S+S   G AK  +    SKKK +GSDED  SPT     E ++A D+DDDDD 
Sbjct: 181 KKEVKKKGSSSAKDGTAKSTI----SKKKGSGSDEDRRSPTHKQIEEKEEAKDEDDDDDD 240

Query: 241 -VQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGV 300
            VQW TDTSL+A++QRI+EQLSAVTADMVML TT+E +  KK+  ++     GS+NGN  
Sbjct: 241 GVQWLTDTSLDASRQRIKEQLSAVTADMVML-TTDEPEKKKKAASNQN---GGSQNGNS- 300

Query: 301 STHGALVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKK 360
             +G +V E+K  LKK   A++L S L +L    QE  +ALVEALFEG  KGF +E +KK
Sbjct: 301 KNYGTVVAEVKANLKKGFGASELLSHLAALPVPAQEKMSALVEALFEGTEKGFGRETLKK 360

Query: 361 KKFLVAVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWY 420
           K +  A  A  EEGSQI+LL +IE FC   +  A KE+ALVLK+LYD DV+EEE ++ WY
Sbjct: 361 KNYFAAAVA--EEGSQILLLHAIEEFCCKPNSNALKEVALVLKTLYDADVLEEEAIVLWY 420

Query: 421 QQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 449
           Q+GL G NK S+IWK+ +PFI+WLQNAESE++EE
Sbjct: 421 QKGLKGDNKNSKIWKNAQPFIDWLQNAESESDEE 443

BLAST of Csor.00g306610 vs. ExPASy Swiss-Prot
Match: P55876 (Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 5.7e-157
Identity = 307/456 (67.32%), Postives = 360/456 (78.95%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMVDIAKAL RPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGI+LVNGAH+TAKLAGLLE FIKKYVQCYGCGNPETEI+I+KTQMI+
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLEVFIKKYVQCYGCGNPETEILISKTQMIS 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKKG KDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDH--SPTRSHAVENDQAADDDDDDDVQW 240
           KKD+ KK S    AK    K  +   + GSDEDH  SPTRSH  + D+AA DDDDDDVQW
Sbjct: 181 KKDAKKKGSKDSTAKGLKKKATT---ATGSDEDHSSSPTRSH--DGDKAAADDDDDDVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNG------- 300
           QTDTS+EAAKQR+QEQLSA TA+MVMLS TEET+   K P H+    NGS          
Sbjct: 241 QTDTSIEAAKQRMQEQLSAATAEMVMLS-TEETEKKMKQPTHKDGSTNGSAKEIPNDKPA 300

Query: 301 -NGVSTHGALVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKE 360
               S +  L+ +IK  L   P+ + LK+ L S T   Q+  NA +EALF GVGKGF+KE
Sbjct: 301 VTKPSPYEELIGDIKASLGSAPTPSQLKAVLASSTLPPQDVMNAPLEALFGGVGKGFTKE 360

Query: 361 VVKKKKFLVAVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFV 420
           VVK KK+L    A  +EG+Q +L+++IE+F    +PEA KE+ +VLK+LYDGD+++EE +
Sbjct: 361 VVKNKKYL--AVAVPDEGAQTLLVQAIEAFGGKCNPEALKEVPVVLKALYDGDILDEETI 420

Query: 421 LEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETE 447
           ++WY   +A A K SQ+ K+ KPF+EWLQ+AES+ E
Sbjct: 421 VDWYNDAVA-AGKDSQVVKNAKPFVEWLQSAESDEE 447

BLAST of Csor.00g306610 vs. ExPASy Swiss-Prot
Match: Q9C8F1 (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX=3702 GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.7e-156
Identity = 307/459 (66.88%), Postives = 364/459 (79.30%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEK G +LVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE++K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKD--SLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQW 240
           KK+  S KK +T+G +K+ V    SKKK      DHSP RS + ENDQA  ++DDDDVQW
Sbjct: 181 KKEAASKKKAATTGTSKDKV----SKKK------DHSPPRSLSDENDQADSEEDDDDVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETK---SSKKSPEHEKSVVNGSKNGNG-- 300
           QTDTS EAA++R++EQLSAVTA+MVMLST EE K     KK+PE         +NGN   
Sbjct: 241 QTDTSREAAEKRMKEQLSAVTAEMVMLSTVEEKKPVAEVKKAPEQV------HENGNSKI 300

Query: 301 -VSTHGALVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVV 360
             + H  LV+EIK+ L    S   LK+ L S +   QE  +AL  ALF G GKGF+KEV+
Sbjct: 301 PENAHEKLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVI 360

Query: 361 KKKKFLVAVAAAQEEG---SQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEF 420
           KKKK+L+A+   QEE    +Q+ LL  IESFC+  S EAAKE+ALV+K LYD D+++E+ 
Sbjct: 361 KKKKYLLALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDV 420

Query: 421 VLEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 449
           ++EWY +G+    K S + K+V PFIEWLQNAESE+EEE
Sbjct: 421 IVEWYNKGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

BLAST of Csor.00g306610 vs. ExPASy Swiss-Prot
Match: Q9S825 (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX=3702 GN=At1g77840 PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.6e-151
Identity = 297/450 (66.00%), Postives = 355/450 (78.89%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNR+DAFYRYKMP+MVTK EG+GNGIKTN++N V+IAKAL RP +YTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+N
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEE +K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTR-SHAVENDQAADDDDDDDVQWQ 240
           K     KK    N K++ T      K++ SDED SP    +A+E D+  D+DDDD V+WQ
Sbjct: 181 K----AKKKALSNGKDSKT-----SKNHSSDEDISPKHDENALEVDE--DEDDDDGVEWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTS EAA++R+ EQLSA TA+MVMLS  E     KK+P+  KS  N  K  N       
Sbjct: 241 TDTSREAAEKRMMEQLSAKTAEMVMLSAME--VEEKKAPK-SKSNGNVVKTENPPPQEKN 300

Query: 301 LVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LV ++K+YLKK    + LKSF+ SL+   Q+  +AL  ALF+GVGKGF+KEV KKK +L 
Sbjct: 301 LVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLA 360

Query: 361 AVAAAQEEGSQIVLLRSIESFC-LNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGL 420
           A A  QE+GSQ+ LL SI +FC  N + EA KE+ALVLK+LYD D+IEEE VL+WY++GL
Sbjct: 361 AAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGL 420

Query: 421 AGANKGSQIWKHVKPFIEWLQNAESETEEE 449
            GA+K S +WK+VKPF+EWLQ+AESE+EEE
Sbjct: 421 TGADKSSPVWKNVKPFVEWLQSAESESEEE 436

BLAST of Csor.00g306610 vs. ExPASy Swiss-Prot
Match: Q9VXK6 (Eukaryotic translation initiation factor 5 OS=Drosophila melanogaster OX=7227 GN=eIF5 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.7e-47
Identity = 152/463 (32.83%), Postives = 224/463 (48.38%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MA  N+  S   D FYRYKMPR+  K+EG+GNGIKT +VNM ++A+A+GRPA Y TKYFG
Sbjct: 1   MATVNVNRS-VTDIFYRYKMPRLQAKVEGKGNGIKTVLVNMAEVARAIGRPATYPTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIIT-KTQMI 120
           CELGAQ+ FD K    +VNG+H+  KL  LL+ FI+K+V C  C NPET + ++ K Q I
Sbjct: 61  CELGAQTLFDHKNERFVVNGSHDVNKLQDLLDGFIRKFVLCPECDNPETNLTVSAKNQTI 120

Query: 121 NMKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKK 180
           +  C ACGF   + +  K+ TFI+KNPP              A+   L EG         
Sbjct: 121 SQSCKACGFHGLLKVNHKVNTFIVKNPPSLNPA---------AQGSSLTEG--------- 180

Query: 181 IKKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTR---------SHAVENDQAADD 240
             K S K+K  + NA  ++T       S G  +  + T          S A+    A DD
Sbjct: 181 --KRSRKQKQKNDNADGSMTNNSLANNSGGESDGGNGTNQASQTEAEISAAIPEKTAKDD 240

Query: 241 DDDDDVQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHE-KSVVNGSK 300
           DD+    W  D S EA + R+Q+ L+     M +    ++T+  +    +E        K
Sbjct: 241 DDEG---WSVDVSKEAIRARLQD-LTDGAKGMTISDDYDKTEKERIDIFYELVKDKRDKK 300

Query: 301 NGNGVSTHGALVHEIK--DYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGF 360
             + V TH  LV E +  D + K P       F E++    Q+    L+          F
Sbjct: 301 QLDDVQTHKELVIEAERLDIINKAPLVLAELLFTENIIKDVQKNRPLLLR---------F 360

Query: 361 SKEVVKKKKFLVAVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEE 420
           +    K +++L+       E  + +L+                ++A + K  YD D+++E
Sbjct: 361 TLNNPKAQRYLIGGVEQTVELHKGILM---------------SKVAGIFKLFYDLDILDE 414

Query: 421 EFVLEWYQ---QGLAGANKGSQIWKHVKPFIEWLQNAESETEE 448
             +LEW Q   +     N  ++I +   PF+ WL+NAE E+ E
Sbjct: 421 AVILEWAQKVSKRHVSKNIAAEIHEKAMPFVLWLKNAEEESSE 414

BLAST of Csor.00g306610 vs. NCBI nr
Match: KAG6598667.1 (Eukaryotic translation initiation factor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 852 bits (2201), Expect = 4.61e-311
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300

Query: 301 VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360
           VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA
Sbjct: 301 VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360

Query: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420
           VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG
Sbjct: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420

Query: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448
           ANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Csor.00g306610 vs. NCBI nr
Match: XP_022962417.1 (probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata] >XP_022962418.1 probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata])

HSP 1 Score: 847 bits (2188), Expect = 4.42e-309
Identity = 445/448 (99.33%), Postives = 446/448 (99.55%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300

Query: 301 VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360
           VHEIKDYLKK+PSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA
Sbjct: 301 VHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360

Query: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420
           VAAAQEEGSQIVLLRSIESFCLNT PE AKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG
Sbjct: 361 VAAAQEEGSQIVLLRSIESFCLNTCPEVAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420

Query: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448
           ANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Csor.00g306610 vs. NCBI nr
Match: KAG7029612.1 (Eukaryotic translation initiation factor 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 846 bits (2185), Expect = 1.31e-308
Identity = 447/449 (99.55%), Postives = 448/449 (99.78%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDD-VQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDD VQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKK+PSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 449

BLAST of Csor.00g306610 vs. NCBI nr
Match: XP_023546001.1 (eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo] >XP_023546002.1 eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 841 bits (2172), Expect = 1.26e-306
Identity = 445/449 (99.11%), Postives = 446/449 (99.33%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDD-VQWQ 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHA ENDQAADDDDDDD VQWQ
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAGENDQAADDDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300

Query: 301 LVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKK+PSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV
Sbjct: 301 LVHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEE VLEWYQQGLA
Sbjct: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEIVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448
           GANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 449

BLAST of Csor.00g306610 vs. NCBI nr
Match: XP_022997142.1 (probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima] >XP_022997143.1 probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima])

HSP 1 Score: 839 bits (2167), Expect = 7.00e-306
Identity = 441/448 (98.44%), Postives = 446/448 (99.55%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHA ENDQAADDDDDDDVQWQT
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAGENDQAADDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGN VS+HGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNRVSSHGAL 300

Query: 301 VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360
           V+EIKDYLK++PSAADLKSFLESLTGTHQETANAL+EALFEGVGKGFSKEVVKKKKFLVA
Sbjct: 301 VNEIKDYLKRSPSAADLKSFLESLTGTHQETANALIEALFEGVGKGFSKEVVKKKKFLVA 360

Query: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420
           VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG
Sbjct: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420

Query: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448
           ANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Csor.00g306610 vs. ExPASy TrEMBL
Match: A0A6J1HCN3 (probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3662 GN=LOC111462865 PE=3 SV=1)

HSP 1 Score: 847 bits (2188), Expect = 2.14e-309
Identity = 445/448 (99.33%), Postives = 446/448 (99.55%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300

Query: 301 VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360
           VHEIKDYLKK+PSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA
Sbjct: 301 VHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360

Query: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420
           VAAAQEEGSQIVLLRSIESFCLNT PE AKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG
Sbjct: 361 VAAAQEEGSQIVLLRSIESFCLNTCPEVAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420

Query: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448
           ANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Csor.00g306610 vs. ExPASy TrEMBL
Match: A0A6J1K464 (probable eukaryotic translation initiation factor 5-1 OS=Cucurbita maxima OX=3661 GN=LOC111492137 PE=3 SV=1)

HSP 1 Score: 839 bits (2167), Expect = 3.39e-306
Identity = 441/448 (98.44%), Postives = 446/448 (99.55%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240
           KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHA ENDQAADDDDDDDVQWQT
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAGENDQAADDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGN VS+HGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNRVSSHGAL 300

Query: 301 VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360
           V+EIKDYLK++PSAADLKSFLESLTGTHQETANAL+EALFEGVGKGFSKEVVKKKKFLVA
Sbjct: 301 VNEIKDYLKRSPSAADLKSFLESLTGTHQETANALIEALFEGVGKGFSKEVVKKKKFLVA 360

Query: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420
           VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG
Sbjct: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420

Query: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448
           ANKGSQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Csor.00g306610 vs. ExPASy TrEMBL
Match: A0A0A0LMB4 (W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 4.27e-276
Identity = 402/448 (89.73%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240
           KKD LKKKST+   K+AVTKVPSKKK+NGSDEDHSPTRSHA +ND+AADD+DDDDVQWQT
Sbjct: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS EHEKS  NG+KNGNGVSTHGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGAL 300

Query: 301 VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360
           + EIKDYLKK  S  DLKSFLESL GT QE  NALVEALF+GVGKGFSKEVVKKKKFL  
Sbjct: 301 IQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAV 360

Query: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420
           VAA   EGSQI+LLR+IESFCLNTSPEA KE+ALVLKSLYDGDVIEEEFVLEWYQQG+AG
Sbjct: 361 VAAV--EGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420

Query: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448
           ANK SQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444

BLAST of Csor.00g306610 vs. ExPASy TrEMBL
Match: A0A6J1BQ30 (eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 GN=LOC111004721 PE=3 SV=1)

HSP 1 Score: 758 bits (1957), Expect = 3.46e-274
Identity = 398/449 (88.64%), Postives = 422/449 (93.99%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDD-DDVQWQ 240
           KKDS+KKKST+GN K+AVTKVPSKKK+NGSDEDHSPTRS A +ND AADDD+D DDVQWQ
Sbjct: 181 KKDSVKKKSTAGNTKDAVTKVPSKKKNNGSDEDHSPTRSQAGDNDHAADDDEDEDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS E+EKS VNG+KNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LVHEIKDYLKK  SAA+LKSFLESL+GT QE  NALVE LF+GVGKGFSKEV+KKK FL 
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLKKKSFLA 360

Query: 361 AVAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLA 420
           A AAAQE  SQI+LLR+IES CLNT+PEA KE+ALVLKSLYDGDV+EEEFVLEWYQQGLA
Sbjct: 361 A-AAAQEGSSQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLA 420

Query: 421 GANKGSQIWKHVKPFIEWLQNAESETEEE 448
           G NK SQIWKHVKPFIEWLQNAESETEEE
Sbjct: 421 GENKSSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Csor.00g306610 vs. ExPASy TrEMBL
Match: A0A1S3BCI3 (eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC103488416 PE=3 SV=1)

HSP 1 Score: 758 bits (1956), Expect = 4.08e-274
Identity = 400/448 (89.29%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240
           KKD LKKKST+   K+AVTKVPSKKK+NGSDEDHSPTRSHA +NDQAADD+DDDDVQWQT
Sbjct: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAADDEDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS EHEKS  NG+KNGNGVSTH +L
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHVSL 300

Query: 301 VHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360
           + EIKD+LKK  S  DLKSFLESL+GT QE  NALVEALF+GVGKGFSKEVVKKKKFL  
Sbjct: 301 IQEIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAV 360

Query: 361 VAAAQEEGSQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420
           VAA  EEGSQI LLRSIESFC+NTSPEA KE+ALVLKSLYDGDVIEEEFVLEWY+QG+AG
Sbjct: 361 VAA--EEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAG 420

Query: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448
           ANK SQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444

BLAST of Csor.00g306610 vs. TAIR 10
Match: AT1G36730.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 307/459 (66.88%), Postives = 364/459 (79.30%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKALGRPAAYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEK G +LVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE++K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKD--SLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQW 240
           KK+  S KK +T+G +K+ V    SKKK      DHSP RS + ENDQA  ++DDDDVQW
Sbjct: 181 KKEAASKKKAATTGTSKDKV----SKKK------DHSPPRSLSDENDQADSEEDDDDVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEETK---SSKKSPEHEKSVVNGSKNGNG-- 300
           QTDTS EAA++R++EQLSAVTA+MVMLST EE K     KK+PE         +NGN   
Sbjct: 241 QTDTSREAAEKRMKEQLSAVTAEMVMLSTVEEKKPVAEVKKAPEQV------HENGNSKI 300

Query: 301 -VSTHGALVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVV 360
             + H  LV+EIK+ L    S   LK+ L S +   QE  +AL  ALF G GKGF+KEV+
Sbjct: 301 PENAHEKLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVI 360

Query: 361 KKKKFLVAVAAAQEEG---SQIVLLRSIESFCLNTSPEAAKEIALVLKSLYDGDVIEEEF 420
           KKKK+L+A+   QEE    +Q+ LL  IESFC+  S EAAKE+ALV+K LYD D+++E+ 
Sbjct: 361 KKKKYLLALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDV 420

Query: 421 VLEWYQQGLAGANKGSQIWKHVKPFIEWLQNAESETEEE 449
           ++EWY +G+    K S + K+V PFIEWLQNAESE+EEE
Sbjct: 421 IVEWYNKGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

BLAST of Csor.00g306610 vs. TAIR 10
Match: AT1G77840.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.1e-152
Identity = 297/450 (66.00%), Postives = 355/450 (78.89%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60
           MALQNIGASNR+DAFYRYKMP+MVTK EG+GNGIKTN++N V+IAKAL RP +YTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120
           CELGAQSKFDEKTG +LVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+N
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEE +K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTR-SHAVENDQAADDDDDDDVQWQ 240
           K     KK    N K++ T      K++ SDED SP    +A+E D+  D+DDDD V+WQ
Sbjct: 181 K----AKKKALSNGKDSKT-----SKNHSSDEDISPKHDENALEVDE--DEDDDDGVEWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGA 300
           TDTS EAA++R+ EQLSA TA+MVMLS  E     KK+P+  KS  N  K  N       
Sbjct: 241 TDTSREAAEKRMMEQLSAKTAEMVMLSAME--VEEKKAPK-SKSNGNVVKTENPPPQEKN 300

Query: 301 LVHEIKDYLKKTPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLV 360
           LV ++K+YLKK    + LKSF+ SL+   Q+  +AL  ALF+GVGKGF+KEV KKK +L 
Sbjct: 301 LVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLA 360

Query: 361 AVAAAQEEGSQIVLLRSIESFC-LNTSPEAAKEIALVLKSLYDGDVIEEEFVLEWYQQGL 420
           A A  QE+GSQ+ LL SI +FC  N + EA KE+ALVLK+LYD D+IEEE VL+WY++GL
Sbjct: 361 AAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGL 420

Query: 421 AGANKGSQIWKHVKPFIEWLQNAESETEEE 449
            GA+K S +WK+VKPF+EWLQ+AESE+EEE
Sbjct: 421 TGADKSSPVWKNVKPFVEWLQSAESESEEE 436

BLAST of Csor.00g306610 vs. TAIR 10
Match: AT5G20920.1 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 56.6 bits (135), Expect = 5.9e-08
Identity = 28/93 (30.11%), Postives = 46/93 (49.46%), Query Frame = 0

Query: 35  KTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENF 94
           KT  VN +D+ K + R   +  +Y   ELG     D +  + +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMINMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

BLAST of Csor.00g306610 vs. TAIR 10
Match: AT5G20920.2 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 56.6 bits (135), Expect = 5.9e-08
Identity = 28/93 (30.11%), Postives = 46/93 (49.46%), Query Frame = 0

Query: 35  KTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENF 94
           KT  VN +D+ K + R   +  +Y   ELG     D +  + +V G        G+L  +
Sbjct: 156 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 215

Query: 95  IKKYVQCYGCGNPETEIIITKTQMINMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 216 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 246

BLAST of Csor.00g306610 vs. TAIR 10
Match: AT5G20920.3 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 56.6 bits (135), Expect = 5.9e-08
Identity = 28/93 (30.11%), Postives = 46/93 (49.46%), Query Frame = 0

Query: 35  KTNVVNMVDIAKALGRPAAYTTKYFGCELGAQSKFDEKTGIALVNGAHETAKLAGLLENF 94
           KT  VN +D+ K + R   +  +Y   ELG     D +  + +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMINMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P487247.9e-15968.72Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5... [more]
P558765.7e-15767.32Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1[more]
Q9C8F11.7e-15666.88Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX... [more]
Q9S8251.6e-15166.00Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX... [more]
Q9VXK61.7e-4732.83Eukaryotic translation initiation factor 5 OS=Drosophila melanogaster OX=7227 GN... [more]
Match NameE-valueIdentityDescription
KAG6598667.14.61e-311100.00Eukaryotic translation initiation factor 5, partial [Cucurbita argyrosperma subs... [more]
XP_022962417.14.42e-30999.33probable eukaryotic translation initiation factor 5-1 [Cucurbita moschata] >XP_0... [more]
KAG7029612.11.31e-30899.55Eukaryotic translation initiation factor 5, partial [Cucurbita argyrosperma subs... [more]
XP_023546001.11.26e-30699.11eukaryotic translation initiation factor 5-like [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022997142.17.00e-30698.44probable eukaryotic translation initiation factor 5-1 [Cucurbita maxima] >XP_022... [more]
Match NameE-valueIdentityDescription
A0A6J1HCN32.14e-30999.33probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3... [more]
A0A6J1K4643.39e-30698.44probable eukaryotic translation initiation factor 5-1 OS=Cucurbita maxima OX=366... [more]
A0A0A0LMB44.27e-27689.73W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=... [more]
A0A6J1BQ303.46e-27488.64eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 G... [more]
A0A1S3BCI34.08e-27489.29eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT1G36730.11.2e-15766.88Translation initiation factor IF2/IF5 [more]
AT1G77840.11.1e-15266.00Translation initiation factor IF2/IF5 [more]
AT5G20920.15.9e-0830.11eukaryotic translation initiation factor 2 beta subunit [more]
AT5G20920.25.9e-0830.11eukaryotic translation initiation factor 2 beta subunit [more]
AT5G20920.35.9e-0830.11eukaryotic translation initiation factor 2 beta subunit [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003307W2 domainSMARTSM00515542_3coord: 356..444
e-value: 9.9E-28
score: 108.1
IPR003307W2 domainPFAMPF02020W2coord: 370..448
e-value: 5.9E-19
score: 68.0
IPR003307W2 domainPROSITEPS51363W2coord: 275..448
score: 23.756828
IPR002735Translation initiation factor IF2/IF5SMARTSM00653eIF2Bneu4coord: 15..129
e-value: 3.5E-63
score: 225.9
IPR002735Translation initiation factor IF2/IF5PFAMPF01873eIF-5_eIF-2Bcoord: 11..127
e-value: 2.2E-36
score: 124.3
NoneNo IPR availableGENE3D3.30.30.170coord: 2..104
e-value: 2.0E-42
score: 145.9
NoneNo IPR availableGENE3D2.20.25.350coord: 105..145
e-value: 2.8E-19
score: 70.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..225
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..190
NoneNo IPR availablePANTHERPTHR23001EUKARYOTIC TRANSLATION INITIATION FACTORcoord: 1..448
NoneNo IPR availablePANTHERPTHR23001:SF28EUKARYOTIC TRANSLATION INITIATION FACTOR 5-1-RELATEDcoord: 1..448
NoneNo IPR availableCDDcd11561W2_eIF5coord: 295..446
e-value: 5.51452E-36
score: 128.118
IPR016021MIF4G-like domain superfamilyGENE3D1.25.40.180coord: 294..448
e-value: 3.9E-26
score: 93.8
IPR016190Translation initiation factor IF2/IF5, zinc-bindingSUPERFAMILY75689Zinc-binding domain of translation initiation factor 2 betacoord: 98..133
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 299..442
IPR016189Translation initiation factor IF2/IF5, N-terminalSUPERFAMILY100966Translation initiation factor 2 beta, aIF2beta, N-terminal domaincoord: 10..101

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g306610.m01Csor.00g306610.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001732 formation of cytoplasmic translation initiation complex
biological_process GO:0001731 formation of translation preinitiation complex
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0006413 translational initiation
cellular_component GO:0005829 cytosol
molecular_function GO:0071074 eukaryotic initiation factor eIF2 binding
molecular_function GO:0005092 GDP-dissociation inhibitor activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0005515 protein binding