Csor.00g301820 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g301820
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationCsor_Chr18: 10831811 .. 10836826 (+)
RNA-Seq ExpressionCsor.00g301820
SyntenyCsor.00g301820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGCAATGGAGAAGGATTTTGATGCTAAGTTGATGATTCAGAAACAGGGGAACGCTTCTAATGGCGGCGGCAATGTTCAGAGATCCAATAGCTTTGCGTTTCGTGCTCCTCAGGAGAATTTTACTATACAGGATTTTGAATTGGGGAAGATTTATGGCGTTGGTTCATATTCCAAGGTAATTTTGTTGCTAAATTTGTTGTTTAATTCGTTTTTTAATTTAATTGTTCTGTTTCTGGATAATCTGTGTTGAATCTGTTTTGATTTGTATATGGATGGGAATTATTGTAATCGGAGTAGCTGTGTGTTCTTGCTCTAAAATTGAACTAATTTGGTAAAAAATGGGGTTAGAAATGAAGTAAAATTCGTTAGAATTCGTATAAACAGAGCTCGATTGTGAGATCTCATATCGATTTGGGAGGAGAACGAAACACCGTTTATAAGGGTGTGGAGACCTCTCCCTAGCTAGGGACCGTTTTAAAAACTTGGAAGGGAAAGCCCAAAGAGGACAATATCTACTAGCGGTGGGCTTGGGTTGTTACAAATGGTATAGAGCCAAACACCGGGCGATGTGTTAGAGAGGAGGCTGAGACCGGAAGGGGGTTGGACATGAGAGGCGATGTGTCAGTGTCGGGTCCTGAAGGGGTGGATTTGGTGGGGGTCTCATATCGATTGGAGGAAGGAACAAGTGCTAGCGAGGACGCTGGGCCCTGAAGGGGGTGGATGGTGAGATTCCACATCAGTTGGAGAGCAGAACAAAACACCCTTTATAAGGGTGTGGAAACCTCTCTCTAGTAGATGCATTTTAAAAATCTTGAGGGGAAGTCCGAAAGGGAAAGTCCAAAGAGGACAATATATGCTAGCGGTGGGCTTGAGTTGTTACAAATGGTATCAGAGCCAGACATCGGGCGATGTGTTAGGGAGGAGGCTGAGACCGGAAGGGGGTTGGACATGAGAGGCGATGTGCCAACGTCAGGTCTTGAAGGGGGTGGATTTGGTGGGGGTCCCACATCGATTGGAGAAATGAACGAGTGCTAGTGAGGACGCTAGACCCGAAGAGGGGTGGATTATGAGATCCCACATTGGTTGGAGAGGAGAACGAAACACTCTTTATAAGGGTATGGAAACCTCTCCCTAGTAGACGCATTTTAAAAATCTTGAGGGGAAGCCCGAAAGGGAAAGCTCAAAGAGGACAATATCTGCTAGCGGTAGGCTTGGGCCATTACATAAACGGAGCTCGATCCCTTGTGCTGAACGGGAAAATTTTAAAAGATTAAGGACTAAATTGAAATTACGGAAAAGATGAAGGACCATTTGGTATATTTAGGCATATTATTGCTTTATAAATGGCCTTTTAATTAATTTTTTTTTTTCATCAGGTTGTGAGGGCGAAGAAGAAGGACACAGGAATTGTGTATGCATTGAAGATAATGGACAAGAAATTCATTACCAAAGAAAACAAAACTGCTTATGTGAAGTTGGAAAGGATTGTACTTGATCAATTGGACCATCCTGGTGTTGTCAGGCTATTTTTTACCTTTCAGGATACTTTTTCTTTGTGTATGTATTTCTTATGCTCGTCTTTTTTTATCGTCTGCTCGTATTCATCTCCTCGCTCTTTTGTTATTTGTGTCGTTTTCTTACTGATACTCGTTTCTTGGTTTTGATATCAAGACATGGCATTGGAGTCGTGTGAAGGTGGAGAGCTTTTTGATCAGATAAATAGGGTAAGTTGATTTGAAAGAGCTTTAGAACACGTGTTTTTATGTCGGGCGATTGCTCGTGTAAGTTTATGAATCCCTGTTGCTATTCTATTGTGTCGTGTGCTTGAACGTTGAAGTCGAGTTAATTGGATTGTTCGATGAATCTATATGCATATGTTTTTGGAGAGAAGTCTTTGGTGAAAGGTTATTAGCATATTTAATTGGTTGTTCGAGTACATGTGAGATTCCATATCGGTTGGAGAGGGGATCGAAACATTTTTTATAAAGGTGTGGAAACCTCTCTCTAGCAGACGTGTTTTAAAACCTTGAGGGGAAGCTCAAAAGGAAAAGCCAAAAGGACAATATCTGCTAATGGTGAGTTTGGTCTGTCACAAATGATATCGGAGCCATACATTAGGCGGTGTGCCAACGAGGACGTTGGCTCCCAAGGGGGTGGATTGTGAGATCCCACCTCGGTTGGAGAGAGGAATAAAAAATTCCTTATAAGGGTGTGAAACATCTTCCTAGTAGACACGTTTTAAAACTTTAAGGGGAATCCCGAAAGGGAAAGTCCAAAGAGGAAAATATCTGTTAGCGGTGGGCTTGGGTTGTTACAAATGGTATCAGAGCTATACACCGGGTGGTGCACCAGCGAGGACGCTGGGCCTCCAAGAGGGGTGGATTGTAAGATCCTACATCGGTTGGAGAGAGGAACGAAACATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGTAATCGTGTCTTAAAACCTTGAGGAGGAGCCCGAAAGGGAAAGTCCAAAGAGAAAAATATCTGCTAGCGGTGGTGGGCTTGAGCGGTTACAAATAGTATCAGAGCTATACACCGAGCGGTGTACTAGCGAGGACGCTGGGCTTCCAAGGGGGGTGAATTGTGAGATCCCACGTTAGTTGGAGAAGGGAACGAAACATTCCTTATTAAGGATGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAACCTTGAGGGGGAGCCTCAAAAGGAAAACCCAAAGAGGACAATATGTGCTAGTAGTGTGCTTTGGCTCTTACAAATGATATAAGAGTCATACACCGAGCGGTGTGCCAGCGAGGACGTTGGGCCTCTAAGAGGGGTGGATTGTGAGATCCCACGTCAGTTGGAGAAGAGAATGAAACATTCTTTATAAAGACGTGGAAACCTCTCCTTAATAGACGTGTTTTAAAAACCTTGAGGGGAAACCCAAAGAGGACAATATCTGCTAGTGGTGGACTAGGACTGTAGGGGCTTCTAGGCAATTTAATGTTTTTTATTCTAAACCTCGAGTATTTTATGAACAGAAAGGTCGACTAGCGGAGGAGGAAGCTCGGTTTTACGCCGCAGAAGTCATCGACGCTCTTGAATACATACACAACATGGGATTGATACACCGAGATATCAAGGTTTCTCATCTATTATATCAGCTCTATTATTGTGGACCTGTGGCAGATCAAACATAACTAACATTTCTTTTGGCAGCCTGAGAACTTGCTGCTTACTACTGATGGACATATCAAAATAGCCGATTTCGGGAGCGTAAAGCCTATGGTCGACAGTCGAATCACCGTGCTTCCGAATGCAGCATCGGGTTTGTTTCGGTTCATGTCACCGAAGACGTGTATATGAACCTCCTTCGAGCGTTTTTTTGACATGGTTTGCACCAATTTTTACATGTAGATGACAAAGCTTGTACATTTGTGGGCACTGCTGCATATGTTCCTCCAGAAGTTCTTAATTCCTCCCCTGCAACTTTCGGGTAACGCGTTCCTCCAACTTACACGTTCATAACGATATTATGTCGGGTATTCGTGTTCGTGTCCGTAACTCATTGGTCCATTTTTCGTGTAAGATAATCTCCCTTTTCGATTTGAAAACAGAAACGACCTTTGGGCGCTCGGGTGCACCGTGTACCAAATGCTTTCAGGGACTTCTCCTTTTAAGGATGCAAGTGAGTGGCTTATTTTTCAAAAAATTGTAGCTCGAGATATAAAATTCCCCAGTTATTTTTCCGAGCAAGCCCGGGATCTCATCGACCACCTATTGGTACGTATGCAATCGAACGATGAATTTTGCTCATAATGATGCACTAAAATCTTTTGATTATGTGATAAAATGTTTACCATATGTTCGACGTTCGAGCTCGGCCTTAACTCGATACTCGACTTTTAATCTACTTCAGGATTTAGATCCCGTTAAGAGACCGGGCGCCGGAGCTGAGGGCTATGCTTCTCTTAAGAGTCATCCATTCTTTGAGGGGGTTGATTGGAAGAACATAAGAACACAAACTCCTCCCAGACTTGCTTTAGAAGCAGTGGTATTGTTTGCTACCAAAAGTGCCTTATTTTCAATGTGTTCTTGCCAAATACATTATGTTGCTTTCGTTTATGTAGTCTCGCTCGAACGAGAACGACGACAGCTCCGAGTCCTCTTGGAATCCTTCGCATATCGGAGACGGTGCACCGAGACAAAACGACGGGAATGCTGGTTCTGCATCGACTTCTGAAGGATCGAATCATATTGCTCGGATTGCATCGATAGACTCCTTCGATTCGAAATGGTATTTGTTTGGATTCGAAGTGATTATAATGACGATTTCGTTCTCGAAAAAACTGAATGTCGGTTTTCGTGTTATCAGGCAACAATTTCTTGAACCTGGAGAATCTGTTCTTATGATATCAAGCGGTGGGTTTGACTGTTACAAAGGCTATCAGAGCTAGTTTCTGGGTGGTGTGTTAGCGAGGACACTGGGCTCCCAAGGGAGGTGGATTGTGAGGTCTCGCATTGGTTGGAGAGGGGAACGAAACATTCCTTATGAGGTGTGGAAACCTCTCCCTAGCACACGCGTGTTAAAATCGTGATATTCACGGCGATACGTAATGGGCCAAAACGGACAATATCTGTTAGCAATGGGCTTGGGTTGTTATAAATGATATCAAAGCTAGTTTTCGGGTGGTGTGTTAGCGAGGATGCCGGGCTCCCAAGGGGGGTGGATGGAGAGGGTAACAAAACATTTCTTATAAGGGTGTGAAAACCTCTCCTTAGCACACGTGTTTCAAAACCGTGATATTAACGACGACACGTAACAGGCCAAAACTGACAATATCTGGTAGCGGTAGGCTTGGGTTGTTACACTTTTGCACAAAATAGCTATCATTTTTTCACAACTATTCTAATACGAAGTCGATTCTTCAGCCGAAAAAGATTGCTTCGTTCGAGGACGCGAAACAACGAGCATGGCAATGGAAGAAGGCGATCGAGGGGCTTCAGAATCGGTGA

mRNA sequence

ATGTTGGCAATGGAGAAGGATTTTGATGCTAAGTTGATGATTCAGAAACAGGGGAACGCTTCTAATGGCGGCGGCAATGTTCAGAGATCCAATAGCTTTGCGTTTCGTGCTCCTCAGGAGAATTTTACTATACAGGATTTTGAATTGGGGAAGATTTATGGCGTTGGTTCATATTCCAAGGTTGTGAGGGCGAAGAAGAAGGACACAGGAATTGTGTATGCATTGAAGATAATGGACAAGAAATTCATTACCAAAGAAAACAAAACTGCTTATGTGAAGTTGGAAAGGATTGTACTTGATCAATTGGACCATCCTGGTGTTGTCAGGCTATTTTTTACCTTTCAGGATACTTTTTCTTTGTACATGGCATTGGAGTCGTGTGAAGGTGGAGAGCTTTTTGATCAGATAAATAGGAAAGGTCGACTAGCGGAGGAGGAAGCTCGGTTTTACGCCGCAGAAGTCATCGACGCTCTTGAATACATACACAACATGGGATTGATACACCGAGATATCAAGCCTGAGAACTTGCTGCTTACTACTGATGGACATATCAAAATAGCCGATTTCGGGAGCGTAAAGCCTATGGTCGACAGTCGAATCACCGTGCTTCCGAATGCAGCATCGGATGACAAAGCTTGTACATTTGTGGGCACTGCTGCATATGTTCCTCCAGAAGTTCTTAATTCCTCCCCTGCAACTTTCGGAAACGACCTTTGGGCGCTCGGGTGCACCGTGTACCAAATGCTTTCAGGGACTTCTCCTTTTAAGGATGCAAGTGAGTGGCTTATTTTTCAAAAAATTGTAGCTCGAGATATAAAATTCCCCAGTTATTTTTCCGAGCAAGCCCGGGATCTCATCGACCACCTATTGGATTTAGATCCCGTTAAGAGACCGGGCGCCGGAGCTGAGGGCTATGCTTCTCTTAAGAGTCATCCATTCTTTGAGGGGGTTGATTGGAAGAACATAAGAACACAAACTCCTCCCAGACTTGCTTTAGAAGCAGTGTCTCGCTCGAACGAGAACGACGACAGCTCCGAGTCCTCTTGGAATCCTTCGCATATCGGAGACGGTGCACCGAGACAAAACGACGGGAATGCTGGTTCTGCATCGACTTCTGAAGGATCGAATCATATTGCTCGGATTGCATCGATAGACTCCTTCGATTCGAAATGGCAACAATTTCTTGAACCTGGAGAATCTCCGAAAAAGATTGCTTCGTTCGAGGACGCGAAACAACGAGCATGGCAATGGAAGAAGGCGATCGAGGGGCTTCAGAATCGGTGA

Coding sequence (CDS)

ATGTTGGCAATGGAGAAGGATTTTGATGCTAAGTTGATGATTCAGAAACAGGGGAACGCTTCTAATGGCGGCGGCAATGTTCAGAGATCCAATAGCTTTGCGTTTCGTGCTCCTCAGGAGAATTTTACTATACAGGATTTTGAATTGGGGAAGATTTATGGCGTTGGTTCATATTCCAAGGTTGTGAGGGCGAAGAAGAAGGACACAGGAATTGTGTATGCATTGAAGATAATGGACAAGAAATTCATTACCAAAGAAAACAAAACTGCTTATGTGAAGTTGGAAAGGATTGTACTTGATCAATTGGACCATCCTGGTGTTGTCAGGCTATTTTTTACCTTTCAGGATACTTTTTCTTTGTACATGGCATTGGAGTCGTGTGAAGGTGGAGAGCTTTTTGATCAGATAAATAGGAAAGGTCGACTAGCGGAGGAGGAAGCTCGGTTTTACGCCGCAGAAGTCATCGACGCTCTTGAATACATACACAACATGGGATTGATACACCGAGATATCAAGCCTGAGAACTTGCTGCTTACTACTGATGGACATATCAAAATAGCCGATTTCGGGAGCGTAAAGCCTATGGTCGACAGTCGAATCACCGTGCTTCCGAATGCAGCATCGGATGACAAAGCTTGTACATTTGTGGGCACTGCTGCATATGTTCCTCCAGAAGTTCTTAATTCCTCCCCTGCAACTTTCGGAAACGACCTTTGGGCGCTCGGGTGCACCGTGTACCAAATGCTTTCAGGGACTTCTCCTTTTAAGGATGCAAGTGAGTGGCTTATTTTTCAAAAAATTGTAGCTCGAGATATAAAATTCCCCAGTTATTTTTCCGAGCAAGCCCGGGATCTCATCGACCACCTATTGGATTTAGATCCCGTTAAGAGACCGGGCGCCGGAGCTGAGGGCTATGCTTCTCTTAAGAGTCATCCATTCTTTGAGGGGGTTGATTGGAAGAACATAAGAACACAAACTCCTCCCAGACTTGCTTTAGAAGCAGTGTCTCGCTCGAACGAGAACGACGACAGCTCCGAGTCCTCTTGGAATCCTTCGCATATCGGAGACGGTGCACCGAGACAAAACGACGGGAATGCTGGTTCTGCATCGACTTCTGAAGGATCGAATCATATTGCTCGGATTGCATCGATAGACTCCTTCGATTCGAAATGGCAACAATTTCTTGAACCTGGAGAATCTCCGAAAAAGATTGCTTCGTTCGAGGACGCGAAACAACGAGCATGGCAATGGAAGAAGGCGATCGAGGGGCTTCAGAATCGGTGA

Protein sequence

MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSKVVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSLYMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTTDGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWALGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGAGAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPRQNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESPKKIASFEDAKQRAWQWKKAIEGLQNR
Homology
BLAST of Csor.00g301820 vs. ExPASy Swiss-Prot
Match: Q4V3C8 (3-phosphoinositide-dependent protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PDPK2 PE=1 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 3.5e-188
Identity = 341/492 (69.31%), Postives = 379/492 (77.03%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           ML M+K+FD+KL +  QGN+S+ G  + RS SFAF+APQENFT  DFELGKIYGVGSYSK
Sbjct: 1   MLTMDKEFDSKLTL--QGNSSSNGETISRSKSFAFKAPQENFTYHDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKD G VYALKIMDKKFITKENKTAYVKLERIVLDQL+HPG+V+LFFTFQDT SL
Sbjct: 61  VVRAKKKDNGTVYALKIMDKKFITKENKTAYVKLERIVLDQLEHPGIVKLFFTFQDTQSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQI RKGRL+E+EARFY+AEV+DALEYIHNMGLIHRDIKPENLLLT 
Sbjct: 121 YMALESCEGGELFDQITRKGRLSEDEARFYSAEVVDALEYIHNMGLIHRDIKPENLLLTL 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPM DS+ITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMQDSQITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCT+YQMLSGTSPFKDASEWLIFQ+I+ARDIKFP++FSE ARDLID LLD DP +RPGA
Sbjct: 241 LGCTLYQMLSGTSPFKDASEWLIFQRIIARDIKFPNHFSEAARDLIDRLLDTDPSRRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           G+EGY SLK HPFF+GVDWKN+R+QTPP+LA +  S+S  + +   S WNP+H+GD +  
Sbjct: 301 GSEGYDSLKRHPFFKGVDWKNLRSQTPPKLAPDPASQS-ASPERDGSPWNPTHVGDTSVL 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDG+ G    SE S  I R+ASIDSFDS+WQQFLEPGES                    
Sbjct: 361 QNDGHNG---LSESSGSITRLASIDSFDSRWQQFLEPGESVLMISAVKKLQKITSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 428
                                                        PKK+ SFEDAKQRA 
Sbjct: 421 ILTNKPRLIYVDPSKLVVKGNIIWSDNSNDLNVQVSSPSHFKICTPKKVLSFEDAKQRAL 480

BLAST of Csor.00g301820 vs. ExPASy Swiss-Prot
Match: Q9XF67 (3-phosphoinositide-dependent protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PDPK1 PE=1 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 1.0e-187
Identity = 339/494 (68.62%), Postives = 382/494 (77.33%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEK+FD+KL++  QGN+SN G NV RS SF+F+APQENFT  DFE GKIYGVGSYSK
Sbjct: 1   MLAMEKEFDSKLVL--QGNSSN-GANVSRSKSFSFKAPQENFTSHDFEFGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKK+TG VYALKIMDKKFITKENKTAYVKLERIVLDQL+HPG+++L+FTFQDT SL
Sbjct: 61  VVRAKKKETGTVYALKIMDKKFITKENKTAYVKLERIVLDQLEHPGIIKLYFTFQDTSSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQI RKGRL+E+EARFY AEV+DALEYIH+MGLIHRDIKPENLLLT+
Sbjct: 121 YMALESCEGGELFDQITRKGRLSEDEARFYTAEVVDALEYIHSMGLIHRDIKPENLLLTS 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPM DS+ITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMQDSQITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCT+YQMLSGTSPFKDASEWLIFQ+I+ARDIKFP++FSE ARDLID LLD +P +RPGA
Sbjct: 241 LGCTLYQMLSGTSPFKDASEWLIFQRIIARDIKFPNHFSEAARDLIDRLLDTEPSRRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSR--SNENDDSSESSWNPSHIGDGA 360
           G+EGY +LK HPFF GVDWKN+R+QTPP+LA +  S+  S E DD+  S WN +HIGD  
Sbjct: 301 GSEGYVALKRHPFFNGVDWKNLRSQTPPKLAPDPASQTASPERDDTHGSPWNLTHIGDSL 360

Query: 361 PRQNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES------------------ 420
             QN+G++   ++SE S  I R+ASIDSFDS+WQQFLEPGES                  
Sbjct: 361 ATQNEGHSAPPTSSESSGSITRLASIDSFDSRWQQFLEPGESVLMISAVKKLQKITSKKV 420

Query: 421 -----------------------------------------------PKKIASFEDAKQR 428
                                                          PKK+ SFEDAKQR
Sbjct: 421 QLILTNKPKLIYVDPSKLVVKGNIIWSDNSNDLNVVVTSPSHFKICTPKKVLSFEDAKQR 480

BLAST of Csor.00g301820 vs. ExPASy Swiss-Prot
Match: O55173 (3-phosphoinositide-dependent protein kinase 1 OS=Rattus norvegicus OX=10116 GN=Pdpk1 PE=1 SV=2)

HSP 1 Score: 285.8 bits (730), Expect = 8.0e-76
Identity = 143/300 (47.67%), Postives = 192/300 (64.00%), Query Frame = 0

Query: 45  QDFELGKIYGVGSYSKVVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDH 104
           +DF+ GKI G GS+S VV A++  T   YA+KI++K+ I KENK  YV  ER V+ +LDH
Sbjct: 83  EDFKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRLDH 142

Query: 105 PGVVRLFFTFQDTFSLYMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNM 164
           P  V+L+FTFQD   LY  L   + GEL   I + G   E   RFY AE++ ALEY+H  
Sbjct: 143 PFFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGK 202

Query: 165 GLIHRDIKPENLLLTTDGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPP 224
           G+IHRD+KPEN+LL  D HI+I DFG+ K        VL   +   +A +FVGTA YV P
Sbjct: 203 GIIHRDLKPENILLNEDMHIQITDFGTAK--------VLSPDSKQARANSFVGTAQYVSP 262

Query: 225 EVLNSSPATFGNDLWALGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARD 284
           E+L    A   +DLWALGC +YQ+++G  PF+  +E+LIFQKI+  +  FP  F  +ARD
Sbjct: 263 ELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARD 322

Query: 285 LIDHLLDLDPVKRPGA-GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDD 344
           L++ LL LD  KR G    EGY  LK+HPFFE + W+N+  QTPP+L     + S +++D
Sbjct: 323 LVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESITWENLHQQTPPKLTAYLPAMSEDDED 374

BLAST of Csor.00g301820 vs. ExPASy Swiss-Prot
Match: Q9Z2A0 (3-phosphoinositide-dependent protein kinase 1 OS=Mus musculus OX=10090 GN=Pdpk1 PE=1 SV=2)

HSP 1 Score: 285.4 bits (729), Expect = 1.0e-75
Identity = 143/300 (47.67%), Postives = 192/300 (64.00%), Query Frame = 0

Query: 45  QDFELGKIYGVGSYSKVVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDH 104
           +DF+ GKI G GS+S VV A++  T   YA+KI++K+ I KENK  YV  ER V+ +LDH
Sbjct: 83  EDFKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRLDH 142

Query: 105 PGVVRLFFTFQDTFSLYMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNM 164
           P  V+L+FTFQD   LY  L   + GEL   I + G   E   RFY AE++ ALEY+H  
Sbjct: 143 PFFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGK 202

Query: 165 GLIHRDIKPENLLLTTDGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPP 224
           G+IHRD+KPEN+LL  D HI+I DFG+ K        VL   +   +A +FVGTA YV P
Sbjct: 203 GIIHRDLKPENILLNEDMHIQITDFGTAK--------VLSPESKQARANSFVGTAQYVSP 262

Query: 225 EVLNSSPATFGNDLWALGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARD 284
           E+L    A   +DLWALGC +YQ+++G  PF+  +E+LIFQKI+  +  FP  F  +ARD
Sbjct: 263 ELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYHFPEKFFPKARD 322

Query: 285 LIDHLLDLDPVKRPGA-GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDD 344
           L++ LL LD  KR G    EGY  LK+HPFFE + W+N+  QTPP+L     + S +++D
Sbjct: 323 LVEKLLVLDATKRLGCEEMEGYGPLKAHPFFETITWENLHQQTPPKLTAYLPAMSEDDED 374

BLAST of Csor.00g301820 vs. ExPASy Swiss-Prot
Match: Q6A1A2 (Putative 3-phosphoinositide-dependent protein kinase 2 OS=Homo sapiens OX=9606 GN=PDPK2P PE=5 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 5.2e-75
Identity = 147/320 (45.94%), Postives = 197/320 (61.56%), Query Frame = 0

Query: 45  QDFELGKIYGVGSYSKVVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDH 104
           +DF+ GKI G GS+S VV A++  T   YA+KI++K+ I KENK  YV  ER V+ +LDH
Sbjct: 53  EDFKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRLDH 112

Query: 105 PGVVRLFFTFQDTFSLYMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNM 164
           P  V+L+FTFQD   LY  L   + GEL   I + G   E   RFY AE++ ALEY+H  
Sbjct: 113 PFFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGK 172

Query: 165 GLIHRDIKPENLLLTTDGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPP 224
           G+IHRD+KPEN+LL  D +I+I DFG+ K        VL   +   +A +FVGTA YV P
Sbjct: 173 GIIHRDLKPENILLNEDMYIQITDFGTAK--------VLSPESKQARANSFVGTAQYVSP 232

Query: 225 EVLNSSPATFGNDLWALGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARD 284
           E+L    A   +DLWALGC +YQ+++G  PF+  +E+LIFQKI+  +  FP  F  +ARD
Sbjct: 233 ELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARD 292

Query: 285 LIDHLLDLDPVKRPGA-GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDD 344
           L++ LL LD  KR G    EGY  LK+HPFFE V W+N+  QTPP+L     + S +++D
Sbjct: 293 LVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTAYLPAMSEDDED 352

Query: 345 S----SESSWNPSHIGDGAP 360
                S   W    +  G P
Sbjct: 353 CYGNVSWPGWRARQVALGPP 364

BLAST of Csor.00g301820 vs. NCBI nr
Match: KAG6573866.1 (3-phosphoinositide-dependent protein kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 853 bits (2203), Expect = 4.41e-312
Identity = 427/427 (100.00%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA
Sbjct: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR
Sbjct: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESPKKIASFEDAKQRAWQWKKA 420
           QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESPKKIASFEDAKQRAWQWKKA
Sbjct: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESPKKIASFEDAKQRAWQWKKA 420

Query: 421 IEGLQNR 427
           IEGLQNR
Sbjct: 421 IEGLQNR 427

BLAST of Csor.00g301820 vs. NCBI nr
Match: XP_022945195.1 (3-phosphoinositide-dependent protein kinase 2-like [Cucurbita moschata])

HSP 1 Score: 822 bits (2124), Expect = 5.64e-299
Identity = 426/492 (86.59%), Postives = 427/492 (86.79%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQINRKGRL+EEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQINRKGRLSEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA
Sbjct: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR
Sbjct: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES                    
Sbjct: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESVLMISMVKKIQKITSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 427
                                                        PKKIASFEDAKQRAW
Sbjct: 421 ILTNKPKLIYVDPSKLIVKGNIIWSDNPNDLNIQVISPSHFKICTPKKIASFEDAKQRAW 480

BLAST of Csor.00g301820 vs. NCBI nr
Match: XP_023542461.1 (3-phosphoinositide-dependent protein kinase 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023542462.1 3-phosphoinositide-dependent protein kinase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 819 bits (2116), Expect = 9.33e-298
Identity = 424/492 (86.18%), Postives = 426/492 (86.59%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQ QGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQNQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQINRKGRL+EEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQINRKGRLSEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA
Sbjct: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GAEGYASLKSHPFFEGVDWKNIRTQTPP+LALEAVSRSNENDDSSESSWNPSHIGDGAPR
Sbjct: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPKLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES                    
Sbjct: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESVLMISMVKKIQKITSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 427
                                                        PKKIASFEDAKQRAW
Sbjct: 421 ILTNKPKLIYVDPSKLIVKGNIIWSDNPNDLNIQVISPSHFKICTPKKIASFEDAKQRAW 480

BLAST of Csor.00g301820 vs. NCBI nr
Match: XP_022968189.1 (3-phosphoinositide-dependent protein kinase 2-like [Cucurbita maxima] >XP_022968190.1 3-phosphoinositide-dependent protein kinase 2-like [Cucurbita maxima])

HSP 1 Score: 811 bits (2096), Expect = 1.04e-294
Identity = 420/492 (85.37%), Postives = 424/492 (86.18%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQ QGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQNQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQINRKGRL+EEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQINRKGRLSEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           +GHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 NGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGC+VYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA
Sbjct: 241 LGCSVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GA+GYASLKSHPFFEGVDWKNIR QTPP LALEAVSRSNENDDSSESSWNPSHIGDGAPR
Sbjct: 301 GADGYASLKSHPFFEGVDWKNIRAQTPPSLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES                    
Sbjct: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESVLMISMVKKIQKITSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 427
                                                        PKKIASFEDAKQRAW
Sbjct: 421 ILTNKPKLIYVDPSKLIVKGNIIWSNNPNDLNIQVISPSHFKICTPKKIASFEDAKQRAW 480

BLAST of Csor.00g301820 vs. NCBI nr
Match: XP_023542463.1 (3-phosphoinositide-dependent protein kinase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795 bits (2054), Expect = 1.23e-288
Identity = 397/400 (99.25%), Postives = 399/400 (99.75%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQ QGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQNQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQINRKGRL+EEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQINRKGRLSEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA
Sbjct: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GAEGYASLKSHPFFEGVDWKNIRTQTPP+LALEAVSRSNENDDSSESSWNPSHIGDGAPR
Sbjct: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPKLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES 400
           QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES
Sbjct: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES 400

BLAST of Csor.00g301820 vs. ExPASy TrEMBL
Match: A0A6J1G087 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111449511 PE=3 SV=1)

HSP 1 Score: 822 bits (2124), Expect = 2.73e-299
Identity = 426/492 (86.59%), Postives = 427/492 (86.79%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQINRKGRL+EEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQINRKGRLSEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA
Sbjct: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR
Sbjct: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES                    
Sbjct: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESVLMISMVKKIQKITSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 427
                                                        PKKIASFEDAKQRAW
Sbjct: 421 ILTNKPKLIYVDPSKLIVKGNIIWSDNPNDLNIQVISPSHFKICTPKKIASFEDAKQRAW 480

BLAST of Csor.00g301820 vs. ExPASy TrEMBL
Match: A0A6J1HWJ2 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111467498 PE=3 SV=1)

HSP 1 Score: 811 bits (2096), Expect = 5.03e-295
Identity = 420/492 (85.37%), Postives = 424/492 (86.18%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQ QGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQNQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQINRKGRL+EEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQINRKGRLSEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           +GHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 NGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGC+VYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA
Sbjct: 241 LGCSVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GA+GYASLKSHPFFEGVDWKNIR QTPP LALEAVSRSNENDDSSESSWNPSHIGDGAPR
Sbjct: 301 GADGYASLKSHPFFEGVDWKNIRAQTPPSLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES                    
Sbjct: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGESVLMISMVKKIQKITSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 427
                                                        PKKIASFEDAKQRAW
Sbjct: 421 ILTNKPKLIYVDPSKLIVKGNIIWSNNPNDLNIQVISPSHFKICTPKKIASFEDAKQRAW 480

BLAST of Csor.00g301820 vs. ExPASy TrEMBL
Match: A0A6J1H2B3 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111458923 PE=3 SV=1)

HSP 1 Score: 770 bits (1989), Expect = 8.92e-279
Identity = 400/492 (81.30%), Postives = 410/492 (83.33%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQ  GN SNG  NV RS SF+FRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQ--GNGSNGA-NVPRSKSFSFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTG VYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGTVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQI RKGRL+EEEARFY AEV+DALEYIHNMGLIHRDIKPENLLLT+
Sbjct: 121 YMALESCEGGELFDQITRKGRLSEEEARFYTAEVVDALEYIHNMGLIHRDIKPENLLLTS 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDP+KRPGA
Sbjct: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPIKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GAEGYASLK+HPFFEGVDWKNIRTQTPPRLALEA + SNE DD SESSWNPSH+GDGA R
Sbjct: 301 GAEGYASLKNHPFFEGVDWKNIRTQTPPRLALEATTNSNETDDGSESSWNPSHLGDGASR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDGNAGSASTSEGSNHI R+ASIDSFDSKWQQFL+PGES                    
Sbjct: 361 QNDGNAGSASTSEGSNHITRLASIDSFDSKWQQFLDPGESVLMISMVKKIQKLSSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 427
                                                        PKKIASFED KQRAW
Sbjct: 421 ILTNKPKLIYVDPSKLIVKGNIIWSDNPNDLNIQVTSPSNFKICTPKKIASFEDVKQRAW 480

BLAST of Csor.00g301820 vs. ExPASy TrEMBL
Match: A0A5A7SZE4 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006960 PE=3 SV=1)

HSP 1 Score: 767 bits (1980), Expect = 2.09e-277
Identity = 402/492 (81.71%), Postives = 411/492 (83.54%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQ  GNASNGG NVQRS SFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQ--GNASNGG-NVQRSKSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQI RKG L+E+EARFY AEV+DALEYIH+MGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQITRKGCLSEDEARFYTAEVVDALEYIHSMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCT+YQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDP KRPGA
Sbjct: 241 LGCTLYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPSKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GAEGYASLKSHPFF+GVDW NIR+QTPPRLALEA S SNENDD SESSWNPSHIGDG  R
Sbjct: 301 GAEGYASLKSHPFFKGVDWNNIRSQTPPRLALEAGSHSNENDDGSESSWNPSHIGDGTSR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDGNAGSASTSEGS+HI R+ASIDSFDSKWQQFLEPGES                    
Sbjct: 361 QNDGNAGSASTSEGSHHITRLASIDSFDSKWQQFLEPGESVLMISMVKKLQKLTSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 427
                                                        PKKIASFEDAKQRAW
Sbjct: 421 ILTNKPKLIYVDPSKLIVKGNIIWSDNPVDLSVQVTSPSHFKICTPKKIASFEDAKQRAW 480

BLAST of Csor.00g301820 vs. ExPASy TrEMBL
Match: A0A1S3BFY8 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103489417 PE=3 SV=1)

HSP 1 Score: 765 bits (1975), Expect = 1.21e-276
Identity = 401/492 (81.50%), Postives = 410/492 (83.33%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEKDFDAKLMIQ  GNASNGG NVQRS SFAFRAPQENFTIQDFELGKIYGVGSYSK
Sbjct: 1   MLAMEKDFDAKLMIQ--GNASNGG-NVQRSKSFAFRAPQENFTIQDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL
Sbjct: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQI RKG L+E+EARFY AEV+DALEYIH+MGLIHRDIKPENLLLTT
Sbjct: 121 YMALESCEGGELFDQITRKGCLSEDEARFYTAEVVDALEYIHSMGLIHRDIKPENLLLTT 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCT+YQMLSGTSPFKDASEWL FQKIVARDIKFPSYFSEQARDLIDHLLDLDP KRPGA
Sbjct: 241 LGCTLYQMLSGTSPFKDASEWLXFQKIVARDIKFPSYFSEQARDLIDHLLDLDPSKRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           GAEGYASLKSHPFF+GVDW NIR+QTPPRLALEA S SNENDD SESSWNPSHIGDG  R
Sbjct: 301 GAEGYASLKSHPFFKGVDWNNIRSQTPPRLALEAGSHSNENDDGSESSWNPSHIGDGTSR 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDGNAGSASTSEGS+HI R+ASIDSFDSKWQQFLEPGES                    
Sbjct: 361 QNDGNAGSASTSEGSHHITRLASIDSFDSKWQQFLEPGESVLMISMVKKLQKLTSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 427
                                                        PKKIASFEDAKQRAW
Sbjct: 421 ILTNKPKLIYVDPSKLIVKGNIIWSDNPVDLSVQVTSPSHFKICTPKKIASFEDAKQRAW 480

BLAST of Csor.00g301820 vs. TAIR 10
Match: AT3G10540.1 (3-phosphoinositide-dependent protein kinase )

HSP 1 Score: 659.1 bits (1699), Expect = 2.5e-189
Identity = 341/492 (69.31%), Postives = 379/492 (77.03%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           ML M+K+FD+KL +  QGN+S+ G  + RS SFAF+APQENFT  DFELGKIYGVGSYSK
Sbjct: 1   MLTMDKEFDSKLTL--QGNSSSNGETISRSKSFAFKAPQENFTYHDFELGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKKD G VYALKIMDKKFITKENKTAYVKLERIVLDQL+HPG+V+LFFTFQDT SL
Sbjct: 61  VVRAKKKDNGTVYALKIMDKKFITKENKTAYVKLERIVLDQLEHPGIVKLFFTFQDTQSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQI RKGRL+E+EARFY+AEV+DALEYIHNMGLIHRDIKPENLLLT 
Sbjct: 121 YMALESCEGGELFDQITRKGRLSEDEARFYSAEVVDALEYIHNMGLIHRDIKPENLLLTL 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPM DS+ITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMQDSQITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCT+YQMLSGTSPFKDASEWLIFQ+I+ARDIKFP++FSE ARDLID LLD DP +RPGA
Sbjct: 241 LGCTLYQMLSGTSPFKDASEWLIFQRIIARDIKFPNHFSEAARDLIDRLLDTDPSRRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSRSNENDDSSESSWNPSHIGDGAPR 360
           G+EGY SLK HPFF+GVDWKN+R+QTPP+LA +  S+S  + +   S WNP+H+GD +  
Sbjct: 301 GSEGYDSLKRHPFFKGVDWKNLRSQTPPKLAPDPASQS-ASPERDGSPWNPTHVGDTSVL 360

Query: 361 QNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES-------------------- 420
           QNDG+ G    SE S  I R+ASIDSFDS+WQQFLEPGES                    
Sbjct: 361 QNDGHNG---LSESSGSITRLASIDSFDSRWQQFLEPGESVLMISAVKKLQKITSKKVQL 420

Query: 421 ---------------------------------------------PKKIASFEDAKQRAW 428
                                                        PKK+ SFEDAKQRA 
Sbjct: 421 ILTNKPRLIYVDPSKLVVKGNIIWSDNSNDLNVQVSSPSHFKICTPKKVLSFEDAKQRAL 480

BLAST of Csor.00g301820 vs. TAIR 10
Match: AT5G04510.1 (3'-phosphoinositide-dependent protein kinase 1 )

HSP 1 Score: 657.5 bits (1695), Expect = 7.2e-189
Identity = 339/494 (68.62%), Postives = 382/494 (77.33%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEK+FD+KL++  QGN+SN G NV RS SF+F+APQENFT  DFE GKIYGVGSYSK
Sbjct: 1   MLAMEKEFDSKLVL--QGNSSN-GANVSRSKSFSFKAPQENFTSHDFEFGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKK+TG VYALKIMDKKFITKENKTAYVKLERIVLDQL+HPG+++L+FTFQDT SL
Sbjct: 61  VVRAKKKETGTVYALKIMDKKFITKENKTAYVKLERIVLDQLEHPGIIKLYFTFQDTSSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQI RKGRL+E+EARFY AEV+DALEYIH+MGLIHRDIKPENLLLT+
Sbjct: 121 YMALESCEGGELFDQITRKGRLSEDEARFYTAEVVDALEYIHSMGLIHRDIKPENLLLTS 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPM DS+ITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMQDSQITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCT+YQMLSGTSPFKDASEWLIFQ+I+ARDIKFP++FSE ARDLID LLD +P +RPGA
Sbjct: 241 LGCTLYQMLSGTSPFKDASEWLIFQRIIARDIKFPNHFSEAARDLIDRLLDTEPSRRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSR--SNENDDSSESSWNPSHIGDGA 360
           G+EGY +LK HPFF GVDWKN+R+QTPP+LA +  S+  S E DD+  S WN +HIGD  
Sbjct: 301 GSEGYVALKRHPFFNGVDWKNLRSQTPPKLAPDPASQTASPERDDTHGSPWNLTHIGDSL 360

Query: 361 PRQNDGNAGSASTSEGSNHIARIASIDSFDSKWQQFLEPGES------------------ 420
             QN+G++   ++SE S  I R+ASIDSFDS+WQQFLEPGES                  
Sbjct: 361 ATQNEGHSAPPTSSESSGSITRLASIDSFDSRWQQFLEPGESVLMISAVKKLQKITSKKV 420

Query: 421 -----------------------------------------------PKKIASFEDAKQR 428
                                                          PKK+ SFEDAKQR
Sbjct: 421 QLILTNKPKLIYVDPSKLVVKGNIIWSDNSNDLNVVVTSPSHFKICTPKKVLSFEDAKQR 480

BLAST of Csor.00g301820 vs. TAIR 10
Match: AT5G04510.2 (3'-phosphoinositide-dependent protein kinase 1 )

HSP 1 Score: 627.9 bits (1618), Expect = 6.1e-180
Identity = 308/393 (78.37%), Postives = 350/393 (89.06%), Query Frame = 0

Query: 1   MLAMEKDFDAKLMIQKQGNASNGGGNVQRSNSFAFRAPQENFTIQDFELGKIYGVGSYSK 60
           MLAMEK+FD+KL++  QGN+SN G NV RS SF+F+APQENFT  DFE GKIYGVGSYSK
Sbjct: 1   MLAMEKEFDSKLVL--QGNSSN-GANVSRSKSFSFKAPQENFTSHDFEFGKIYGVGSYSK 60

Query: 61  VVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLDHPGVVRLFFTFQDTFSL 120
           VVRAKKK+TG VYALKIMDKKFITKENKTAYVKLERIVLDQL+HPG+++L+FTFQDT SL
Sbjct: 61  VVRAKKKETGTVYALKIMDKKFITKENKTAYVKLERIVLDQLEHPGIIKLYFTFQDTSSL 120

Query: 121 YMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHNMGLIHRDIKPENLLLTT 180
           YMALESCEGGELFDQI RKGRL+E+EARFY AEV+DALEYIH+MGLIHRDIKPENLLLT+
Sbjct: 121 YMALESCEGGELFDQITRKGRLSEDEARFYTAEVVDALEYIHSMGLIHRDIKPENLLLTS 180

Query: 181 DGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240
           DGHIKIADFGSVKPM DS+ITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA
Sbjct: 181 DGHIKIADFGSVKPMQDSQITVLPNAASDDKACTFVGTAAYVPPEVLNSSPATFGNDLWA 240

Query: 241 LGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQARDLIDHLLDLDPVKRPGA 300
           LGCT+YQMLSGTSPFKDASEWLIFQ+I+ARDIKFP++FSE ARDLID LLD +P +RPGA
Sbjct: 241 LGCTLYQMLSGTSPFKDASEWLIFQRIIARDIKFPNHFSEAARDLIDRLLDTEPSRRPGA 300

Query: 301 GAEGYASLKSHPFFEGVDWKNIRTQTPPRLALEAVSR--SNENDDSSESSWNPSHIGDGA 360
           G+EGY +LK HPFF GVDWKN+R+QTPP+LA +  S+  S E DD+  S WN +HIGD  
Sbjct: 301 GSEGYVALKRHPFFNGVDWKNLRSQTPPKLAPDPASQTASPERDDTHGSPWNLTHIGDSL 360

Query: 361 PRQNDGNAGSASTSEGSNHIARIASIDSFDSKW 392
             QN+G++   ++SE S  I R+ASIDSFDS+W
Sbjct: 361 ATQNEGHSAPPTSSESSGSITRLASIDSFDSRW 390

BLAST of Csor.00g301820 vs. TAIR 10
Match: AT3G08730.1 (protein-serine kinase 1 )

HSP 1 Score: 221.1 bits (562), Expect = 1.7e-57
Identity = 103/282 (36.52%), Postives = 169/282 (59.93%), Query Frame = 0

Query: 44  IQDFELGKIYGVGSYSKVVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLD 103
           I DFE+ K+ G G++ KV + +KK+T  +YA+K+M K  I ++N   Y+K ER +L ++D
Sbjct: 131 IDDFEVMKVVGKGAFGKVYQVRKKETSEIYAMKVMRKDHIMEKNHAEYMKAERDILTKID 190

Query: 104 HPGVVRLFFTFQDTFSLYMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHN 163
           HP +V+L ++FQ  + LY+ L+   GG LF Q+  +G   E+ AR Y AE++ A+ ++H 
Sbjct: 191 HPFIVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEIVSAVSHLHE 250

Query: 164 MGLIHRDIKPENLLLTTDGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVP 223
            G++HRD+KPEN+L+ TDGH+ + DFG  K   +           + ++ +  GT  Y+ 
Sbjct: 251 KGIMHRDLKPENILMDTDGHVMLTDFGLAKEFEE-----------NTRSNSMCGTTEYMA 310

Query: 224 PEVLNSSPATFGNDLWALGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQAR 283
           PE++         D W++G  +Y+ML+G  PF   S+  I QKIV   IK P + S +A 
Sbjct: 311 PEIVRGKGHDKAADWWSVGILLYEMLTGKPPFL-GSKGKIQQKIVKDKIKLPQFLSNEAH 370

Query: 284 DLIDHLLDLDPVKRPGAGAEGYASLKSHPFFEGVDWKNIRTQ 326
            ++  LL  +P +R G+G  G   +K H +F+G++WK +  +
Sbjct: 371 AILKGLLQKEPERRLGSGLSGAEEIKQHKWFKGINWKKLEAR 400

BLAST of Csor.00g301820 vs. TAIR 10
Match: AT3G08720.1 (serine/threonine protein kinase 2 )

HSP 1 Score: 219.2 bits (557), Expect = 6.5e-57
Identity = 103/282 (36.52%), Postives = 168/282 (59.57%), Query Frame = 0

Query: 44  IQDFELGKIYGVGSYSKVVRAKKKDTGIVYALKIMDKKFITKENKTAYVKLERIVLDQLD 103
           I+DFE+ K+ G G++ KV + +KKDT  +YA+K+M K  I ++N   Y+K ER +L ++D
Sbjct: 137 IEDFEVLKVVGQGAFGKVYQVRKKDTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTKID 196

Query: 104 HPGVVRLFFTFQDTFSLYMALESCEGGELFDQINRKGRLAEEEARFYAAEVIDALEYIHN 163
           HP +V+L ++FQ  + LY+ L+   GG LF Q+  +G   E+ AR Y AE++ A+ ++H 
Sbjct: 197 HPFIVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEIVSAVSHLHE 256

Query: 164 MGLIHRDIKPENLLLTTDGHIKIADFGSVKPMVDSRITVLPNAASDDKACTFVGTAAYVP 223
            G++HRD+KPEN+L+  DGH+ + DFG  K   +           + ++ +  GT  Y+ 
Sbjct: 257 KGIMHRDLKPENILMDVDGHVMLTDFGLAKEFEE-----------NTRSNSMCGTTEYMA 316

Query: 224 PEVLNSSPATFGNDLWALGCTVYQMLSGTSPFKDASEWLIFQKIVARDIKFPSYFSEQAR 283
           PE++         D W++G  +Y+ML+G  PF   S+  I QKIV   IK P + S +A 
Sbjct: 317 PEIVRGKGHDKAADWWSVGILLYEMLTGKPPFL-GSKGKIQQKIVKDKIKLPQFLSNEAH 376

Query: 284 DLIDHLLDLDPVKRPGAGAEGYASLKSHPFFEGVDWKNIRTQ 326
            L+  LL  +P +R G+G  G   +K H +F+ ++WK +  +
Sbjct: 377 ALLKGLLQKEPERRLGSGPSGAEEIKKHKWFKAINWKKLEAR 406

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4V3C83.5e-18869.313-phosphoinositide-dependent protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9XF671.0e-18768.623-phosphoinositide-dependent protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN... [more]
O551738.0e-7647.673-phosphoinositide-dependent protein kinase 1 OS=Rattus norvegicus OX=10116 GN=P... [more]
Q9Z2A01.0e-7547.673-phosphoinositide-dependent protein kinase 1 OS=Mus musculus OX=10090 GN=Pdpk1 ... [more]
Q6A1A25.2e-7545.94Putative 3-phosphoinositide-dependent protein kinase 2 OS=Homo sapiens OX=9606 G... [more]
Match NameE-valueIdentityDescription
KAG6573866.14.41e-312100.003-phosphoinositide-dependent protein kinase 1, partial [Cucurbita argyrosperma s... [more]
XP_022945195.15.64e-29986.593-phosphoinositide-dependent protein kinase 2-like [Cucurbita moschata][more]
XP_023542461.19.33e-29886.183-phosphoinositide-dependent protein kinase 2-like isoform X1 [Cucurbita pepo su... [more]
XP_022968189.11.04e-29485.373-phosphoinositide-dependent protein kinase 2-like [Cucurbita maxima] >XP_022968... [more]
XP_023542463.11.23e-28899.253-phosphoinositide-dependent protein kinase 1-like isoform X2 [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
A0A6J1G0872.73e-29986.59Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1HWJ25.03e-29585.37Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1H2B38.92e-27981.30Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A5A7SZE42.09e-27781.71Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BFY81.21e-27681.50Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT3G10540.12.5e-18969.313-phosphoinositide-dependent protein kinase [more]
AT5G04510.17.2e-18968.623'-phosphoinositide-dependent protein kinase 1 [more]
AT5G04510.26.1e-18078.373'-phosphoinositide-dependent protein kinase 1 [more]
AT3G08730.11.7e-5736.52protein-serine kinase 1 [more]
AT3G08720.16.5e-5736.52serine/threonine protein kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 407..427
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 131..326
e-value: 2.8E-96
score: 323.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 45..130
e-value: 2.8E-96
score: 323.9
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 45..247
e-value: 5.7E-26
score: 88.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 334..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 337..355
NoneNo IPR availablePANTHERPTHR24356:SF3863-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1coord: 21..406
NoneNo IPR availablePANTHERPTHR24356SERINE/THREONINE-PROTEIN KINASEcoord: 21..406
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 47..314
e-value: 3.5E-89
score: 312.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 49..314
e-value: 3.9E-65
score: 219.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 47..314
score: 48.314972
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 166..178
IPR039046PDPK1 familyCDDcd05581STKc_PDK1coord: 45..314
e-value: 7.40008E-130
score: 373.859
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 44..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g301820.m01Csor.00g301820.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity