Csor.00g300690 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g300690
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionFAD synthase
LocationCsor_Chr18: 9965942 .. 9970113 (+)
RNA-Seq ExpressionCsor.00g300690
SyntenyCsor.00g300690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGCCGCCGGCGCTCACGTTTCCCACCACCTCCGGGAGTGCGACTGCCACTTCGGCTTCCGGTTAAACTCAGGCCTTCCCTCCTCCTCCTCTTCCGCCGTCGTCCCCTTTCCTCCGATATGGCCTCGGAAGCCCACTTCTTCCCCTTCAATTTCTCACCGCTCCCAACGACGTGGGTACTCTTTCTTTTGCTCCATTGTCCCCTCCACCGCCGCCGGCGAACCCCCCATTCTTTCCGATTGTTTTGGGTAACTTTCAATTTTGTTCTTCTTTGATTGTTGTCTGTGTTGCTTTCTAAAATTACCTGTTGTTTCTTTGTTTCCCAGTTATCGAGGAGGTGGTCGGGAAGTCTCTGTGACAGGTTTGTTTTCTCCTCGTTAATTTTAAGGAAGAATGTTAATTCGTATCTTTGTCTGAGTTTTTTCTGCGTGTTTTATGTGAGGGAAGTTTTTAGTCCTTTTGGGTTGTCTATATATGGTTGTGTTCTTGTAATGCTGGTTGAAAGTTGTCTTAATCTATATACAATATGAATTTGGGAATTTGGGTTGTCACACTCACTTTTCTTAGTAGCGGACTTTTGCGGTTGTGCGGCACCTACTCCCAATACTGAGTGAGTCAAGCCAATTTGGATCTGTGTCGCCTACTGTCGAACAATGTATGCTTAAGCCTCTGGCCCTGTTTTGAGCGGAAGGTTTTAGAAAATGCGGGTTGAAAGAGAGTTTGAAAGGAAGGTTGAAAGAAAGTTTGAGAGTCGTTATATGACTATAAGAAAAGAAAGAAACACGGTAGCTTAAATATCATATCACTCGTGTAGGAAGAGTTGACAATTAGTTGCATCTCCCTATCGTACATTTTGAAGCGAATCAGTCTGGCAGGCCTATACATGATTTTGTCGAACAGGCAACGTATGTTCAAGCCTCTGGCCTCGTTTTGAGAGGAAGGTTTTAGAAAACGGGGTAGGGAGATTTTTGAAAGAGAGTTTGATAGGAAGGTTGAAAGAAAGTTTGAAAGTCGTTATAGGGCTATAAGAAAAGAAAGAAACACTGTAGCTTAAATATCATATAACTCGTGTGGGAAGAATTGACAATTAGTCGCATCCTACTATAGTACATTTTGAGGCGACTCAGGTCTGGTAGACCTAGACATGATTATACAAAATCTAAGTGGAAGGAGTTGACGACTGATCGCATCTTCTTATAGTACATTTTGGGTCGATTCAGGTCGAGCAAGCCTAAACGTGATTTCGCCGAATAGTCATACAAACAGAACAACTGCCTGAAAAGTATTACTTAAATGGAAATCACGCCCCTTTTTCTTTTTCCAACGTGACGAAAGAAAGCATGGTTGAATAATGACTTCACTTCGATCTCTTCGACCGAACAATATACATCACAAGTCTTGTGATAAGAAATCCAACCACCAATACTGATAGAACAAAAAAAATGCCCATTCCCCACTCAAAATGAGCCTTCGACGAGACGTGGACATTCCCCAACTCGAATTAGATCTTGGAAATGATCGACTCAAATAGATCATAAAAACCCACTTCACGAAGATCTCTTCCTTCTCTTCGGGCTTAGGAGACCATCCTTTTGTTGTGGCATGCTCTTGAGATTTACAGATTCATAGGGGGCCCCGTCAGTCCGACAGATCAAAATCCACTAAAAACATACATAGATAAGATCTCATTGTCCGAGAAATGCTCTTTTCCCGAAACAAACGAAAGAAAAACATCATTTTATCCAATTATGTTTTTGTAATGATTTGTTATCAATCATAGGGGGAATAGTAGCATTAGGAAAATCTGATGCTCTCCATGTTGGTCATCGAGAGCTTGCAATTCAAGCATCAAAGCTTGGATCTCCATTTCTATTGTCATTTGTTGGAATTGCTCAAGTACTTGGTTGGGAACCTAGGTACGCATAATGCCTTTTCTTTATGTAACTTATTGTTTGTTTCATCATAAACAGCACGACAAGTATGTCCGACCATGGATTTTATGTCGTTTTCGTTGTGTTATAAGTTTTTGACATGTTCGTGGGTCGACATCATGCAACTATCCCATGTTTTTTCGTGGAAGGTTTGCTCATTTTATGTGAGATCCCACATTAGTTGGAGAGAGGATGAAACATTCCTTATAAGGGTGTGGAAACGTCTCCCTAGTACACGCGTTTTAAAACCGTGAGACTTATGGTGATACGTAACGGGCCAAAGCAGACACTATCTGCTAGCGGTTGGCTTGGGCTGTTACAAATGGTATCAGAGCTAGACATCGGGCGGTGTGTTAGTGAGGATGCTGGGCCCCCATGAGAGTGGACTGTGAGATCCCACGTCGGTTGGAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGAGAATCTCTCCCTAGTAGACGCGTTTTAAAACTGTGAGGCTAATGGCGATACGTAACGGGGCAAAGCAGACAATATCTACTAGTGGTGGGCTTGGGCCGTTACAAATAGTATCAGAGCTAGACATCGGGCGGTGTGTTAGCGAGGACACTGGGCTCCCAAGGGGGTGGATTGTGAGGTCTCACATCAGTTGGAGAGGGGAACAAAGCATTCCTTATAAGGGCGTGAAAATCTCTCCCTAGTTGACGCGTTTTAAAACCGTGAAGCTGGCGGCGATACATAACGGGCCAAAGCGGACAATATTTGCTAGCGGTGGGCTTGGGCTATTGCAAATGGTATTAGAACTAGACACCAGGCGGTGTGCTAGTGAGGACGCTGGGTTCTCAAGGGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGGAACGACGCATTCCTTATGAGGGTGTGGAAACTTCTCCCTAGTAGACGCGTTTAAAAACCGTGAGGCTGACGGTCATACGTAATGGGCCAAAGCGAACAATATCTACTAGTGGTGGACTTGGGCTATTACAAATGGTATCAGAGCTAGACACCGGGCGATGTGCTAGCGAGGGCTGGGCCCCCAAGGGGGGTGGATTGTGAGATCCTACATCGGTTGGAGAGGGGAACGGAGCATTCCTTATAAGGGTGTGGAAACCTCTCATTACTAAACATGTTTTAAAACCGTGAGACTGATGGCGATGCGTAATGGGTTAAAACAGACAATATCTACTAGCGGTGGACTCGAGCTATTACATTTTAATTGTTTTTGTTACCTAGAATTGTTTGACCGTCTTTCTCCATCGAAGAGGCAAAACATAATCAACCATTGGCTAGCGCTCATCTCTAGCTCTGGTTCCAGCCTTTCATTTCATATACCATTTCCGTATGCCATACCTCTTATTCTTTCTTGCTCAACGTCCAAGTCTCGTGCTGTTTCTCTACCTGTTGCATCTGTTCTTAACAATCACCAAGAATTATATGCTTGGAGTTAATTATTTATCTGTTAGTTAACTCGAACAGGGCTCCCGTAGTCGCTCAATGCGACAGGACTCGAGTTCTTTCCTCGTGGGCACCATTCTGCAGAAACATGGCTCCATCAGAATATCAGATTCAATTTTCAAGTGTTCGTCATCTAACTCCACGAGAATTTGTCGTGAAACTCTCGAACGAGCTCGGTGTTCGTGGAGTTGTGGCAGGTAAATGAATTTCGACATATCGGTAAAATATCCTCTCTTGGAATTGCTGTAAAATGTAGCCTTCGGTGGCAGGTGAAAGTTATAGATTTGGATATAAAGCAGCAGGCGACGCAGCCGAGTTGGTGAAACTGTGCAACGAGTACGGGGTAGGCGCTTATATCATAAAATCCGTGATGGATAAGAACCAAGAAGCTACGAATTCAACCAATTCAAAGGAGCGAGGACAAGTGTCGTCTACTCGGGTTCGCTACGCCCTTTCGACAGGTGATATGAAATACGTCTCCGAGCTTTTAGGCCGTAGACATCGTCTAGTTTTGATGGCGAAAGACGTAGAGGGATTAAGTTATAGCAATAGTAGAGTGTCAGTTCCAAGATCATGTTCATTAAACTTAGCACCAAAAGAAGGTTTATATGATAAGTGCTTTGTCTGGTCGTTTGATCAGAACTTGATTCCATGTCGGGTCGTTATTGACTCGAGTCATGTTCATATCGAGTTAGATGAGTTAGCTACGTGTCGTCATCCTGTAGGAACCGATGACTACATAAGTGTTGAGTTTGACGATGAAGGTGTATGA

mRNA sequence

ATGTTGGCCGCCGGCGCTCACGTTTCCCACCACCTCCGGGAGTGCGACTGCCACTTCGGCTTCCGGTTAAACTCAGGCCTTCCCTCCTCCTCCTCTTCCGCCGTCGTCCCCTTTCCTCCGATATGGCCTCGGAAGCCCACTTCTTCCCCTTCAATTTCTCACCGCTCCCAACGACGTGGGTACTCTTTCTTTTGCTCCATTGTCCCCTCCACCGCCGCCGGCGAACCCCCCATTCTTTCCGATTGTTTTGGTTATCGAGGAGGTGGTCGGGAAGTCTCTGTGACAGGGGGAATAGTAGCATTAGGAAAATCTGATGCTCTCCATGTTGGTCATCGAGAGCTTGCAATTCAAGCATCAAAGCTTGGATCTCCATTTCTATTGTCATTTGTTGGAATTGCTCAAGTACTTGGTTGGGAACCTAGGGCTCCCGTAGTCGCTCAATGCGACAGGACTCGAGTTCTTTCCTCGTGGGCACCATTCTGCAGAAACATGGCTCCATCAGAATATCAGATTCAATTTTCAAGTGTTCGTCATCTAACTCCACGAGAATTTGTCGTGAAACTCTCGAACGAGCTCGGTGTTCGTGGAGTTGTGGCAGGTGAAAGTTATAGATTTGGATATAAAGCAGCAGGCGACGCAGCCGAGTTGGTGAAACTGTGCAACGAGTACGGGGTAGGCGCTTATATCATAAAATCCGTGATGGATAAGAACCAAGAAGCTACGAATTCAACCAATTCAAAGGAGCGAGGACAAGTGTCGTCTACTCGGGTTCGCTACGCCCTTTCGACAGGTGATATGAAATACGTCTCCGAGCTTTTAGGCCGTAGACATCGTCTAGTTTTGATGGCGAAAGACGTAGAGGGATTAAGTTATAGCAATAGTAGAGTGTCAGTTCCAAGATCATGTTCATTAAACTTAGCACCAAAAGAAGGTTTATATGATAAGTGCTTTGTCTGGTCGTTTGATCAGAACTTGATTCCATGTCGGGTCGTTATTGACTCGAGTCATGTTCATATCGAGTTAGATGAGTTAGCTACGTGTCGTCATCCTGTAGGAACCGATGACTACATAAGTGTTGAGTTTGACGATGAAGGTGTATGA

Coding sequence (CDS)

ATGTTGGCCGCCGGCGCTCACGTTTCCCACCACCTCCGGGAGTGCGACTGCCACTTCGGCTTCCGGTTAAACTCAGGCCTTCCCTCCTCCTCCTCTTCCGCCGTCGTCCCCTTTCCTCCGATATGGCCTCGGAAGCCCACTTCTTCCCCTTCAATTTCTCACCGCTCCCAACGACGTGGGTACTCTTTCTTTTGCTCCATTGTCCCCTCCACCGCCGCCGGCGAACCCCCCATTCTTTCCGATTGTTTTGGTTATCGAGGAGGTGGTCGGGAAGTCTCTGTGACAGGGGGAATAGTAGCATTAGGAAAATCTGATGCTCTCCATGTTGGTCATCGAGAGCTTGCAATTCAAGCATCAAAGCTTGGATCTCCATTTCTATTGTCATTTGTTGGAATTGCTCAAGTACTTGGTTGGGAACCTAGGGCTCCCGTAGTCGCTCAATGCGACAGGACTCGAGTTCTTTCCTCGTGGGCACCATTCTGCAGAAACATGGCTCCATCAGAATATCAGATTCAATTTTCAAGTGTTCGTCATCTAACTCCACGAGAATTTGTCGTGAAACTCTCGAACGAGCTCGGTGTTCGTGGAGTTGTGGCAGGTGAAAGTTATAGATTTGGATATAAAGCAGCAGGCGACGCAGCCGAGTTGGTGAAACTGTGCAACGAGTACGGGGTAGGCGCTTATATCATAAAATCCGTGATGGATAAGAACCAAGAAGCTACGAATTCAACCAATTCAAAGGAGCGAGGACAAGTGTCGTCTACTCGGGTTCGCTACGCCCTTTCGACAGGTGATATGAAATACGTCTCCGAGCTTTTAGGCCGTAGACATCGTCTAGTTTTGATGGCGAAAGACGTAGAGGGATTAAGTTATAGCAATAGTAGAGTGTCAGTTCCAAGATCATGTTCATTAAACTTAGCACCAAAAGAAGGTTTATATGATAAGTGCTTTGTCTGGTCGTTTGATCAGAACTTGATTCCATGTCGGGTCGTTATTGACTCGAGTCATGTTCATATCGAGTTAGATGAGTTAGCTACGTGTCGTCATCCTGTAGGAACCGATGACTACATAAGTGTTGAGTTTGACGATGAAGGTGTATGA

Protein sequence

MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRGYSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASKLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLTPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVEFDDEGV
Homology
BLAST of Csor.00g300690 vs. ExPASy Swiss-Prot
Match: Q8VZR0 (FAD synthetase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF2 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-92
Identity = 189/372 (50.81%), Postives = 248/372 (66.67%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60
           ML  G+ V  HL + + H    L  G      + +V     + R P+ + + SH   R+ 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGF---CGAKIVQLSSFFLR-PSQAMAKSHHFSRKL 60

Query: 61  YSFFCSIVPS--TAAGEPPILSDCFGYRGGGREV------SVTGGIVALGKSDALHVGHR 120
                S   S    +GE PIL +CF  R    E+       V+GGIVALGK DALH+GHR
Sbjct: 61  RQRMISSFGSHCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHR 120

Query: 121 ELAIQASKLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQ 180
           EL IQAS++G+P+LLSFVG+A+VLGWEPRAP+VA+CDR RVL+SWA +C + AP EY+I+
Sbjct: 121 ELTIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIE 180

Query: 181 FSSVRHLTPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKS 240
           F+SVRHLTPR+FV KLS EL V GVVAGE+YRFGYKA+GDA+ELV+LC E G+ A II S
Sbjct: 181 FASVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINS 240

Query: 241 VMDKNQEAT--NSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLS 300
           VMD  Q +   +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRL+L  +  +   
Sbjct: 241 VMDMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--M 300

Query: 301 YSNSRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHP 360
            S   +SVPRS  LNL P  G+Y  C +   D++ +PC VV+D+S++H+E +E+  C   
Sbjct: 301 PSERMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEEVRLCNLD 360

Query: 361 VGTD-DYISVEF 362
              +   +SVEF
Sbjct: 361 WSQEFRLVSVEF 366

BLAST of Csor.00g300690 vs. ExPASy Swiss-Prot
Match: Q9FMW8 (FAD synthetase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF1 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.6e-85
Identity = 157/277 (56.68%), Postives = 212/277 (76.53%), Query Frame = 0

Query: 77  PILSDCFGYRGGGREVS------VTGGIVALGKSDALHVGHRELAIQASKLGSPFLLSFV 136
           P   DCF       E+S      V GGIVALGK DALH+GHRELAIQA+++G+P+LLSFV
Sbjct: 59  PAPLDCFSQGDDHPELSAEGLSPVAGGIVALGKFDALHIGHRELAIQAARIGTPYLLSFV 118

Query: 137 GIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLTPREFVVKLSN 196
           G+A+VLGW+PRAP+VA+CDR RVLSSWA +C N+AP E++I+F+SVRHL P++FV KLS 
Sbjct: 119 GLAEVLGWKPRAPIVAKCDRKRVLSSWASYCGNIAPVEFEIEFASVRHLNPQQFVEKLSR 178

Query: 197 ELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEATNS----TNS 256
           EL V GVVAGE+YRFGY+A+GDA+ELV+LC ++G+ AYII SVMDKNQ + N+    + S
Sbjct: 179 ELRVCGVVAGENYRFGYRASGDASELVRLCKDFGISAYIINSVMDKNQVSVNTEEEDSKS 238

Query: 257 KERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPRSCSLNL 316
           KERGQVSSTRVR+AL+ GD++YV+ELLGR HR++   +  + L+    R+S+  S  LNL
Sbjct: 239 KERGQVSSTRVRHALAAGDVRYVTELLGRPHRVISRTR-TQDLTSKRGRISLQTSSLLNL 298

Query: 317 APKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDE 344
            P  G+Y  C +   D++ I C+V++D+S+++IE +E
Sbjct: 299 PPGNGVYKACSLIVGDKHPISCKVIVDTSNLYIETEE 334

BLAST of Csor.00g300690 vs. ExPASy Swiss-Prot
Match: Q10BX9 (FAD synthetase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBF PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.1e-84
Identity = 160/288 (55.56%), Postives = 214/288 (74.31%), Query Frame = 0

Query: 78  ILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASKLGSPFLLSFVGIAQVLG 137
           +L DC     G  +  V  GIVALGK DALH+GHRELA+ ASK G+PFLLSFVGIA+VLG
Sbjct: 111 LLIDC-----GEDQDCVIDGIVALGKFDALHIGHRELAMYASKAGTPFLLSFVGIAEVLG 170

Query: 138 WEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLTPREFVVKLSNELGVRGV 197
           WE R P+VAQCDR RVL+SWAP+C+N+ P EYQ++FS VR+LTPR+FV +LS +L ++GV
Sbjct: 171 WEYRPPIVAQCDRKRVLTSWAPYCKNVVPIEYQVEFSKVRYLTPRQFVERLSRDLKIQGV 230

Query: 198 VAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQE---ATNSTNSKERGQVSS 257
           VAGE+YRFGY+A+GDAAELVKLC E+G+ A+I++SVMD  +     T S NS ++GQVSS
Sbjct: 231 VAGENYRFGYRASGDAAELVKLCEEFGLSAFIVRSVMDTARSYNGVTTSVNSSDKGQVSS 290

Query: 258 TRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPRSCSLNLAPKEGLYD 317
           +RVR+AL+ GDM+YVSELLGR+HRLVL  K  E       R+ +P+SC LN+ P +GLY+
Sbjct: 291 SRVRHALAMGDMEYVSELLGRKHRLVLTVK--ENHLQERKRIMLPKSCMLNMPPADGLYE 350

Query: 318 KCFVWSFDQNLIPCRVVIDSSHVHIEL-DELATCRHPVGTDDYISVEF 362
            C + +   +L  CRV+I+S  + IE+ DE +   + +  +  + +EF
Sbjct: 351 NCDLLN-GGHLGLCRVIINSETIVIEMKDENSLLPNTIQENQQLGIEF 390

BLAST of Csor.00g300690 vs. ExPASy Swiss-Prot
Match: P57250 (Bifunctional riboflavin kinase/FMN adenylyltransferase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=ribF PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-07
Identity = 47/188 (25.00%), Postives = 94/188 (50.00%), Query Frame = 0

Query: 95  TGGIVALGKSDALHVGHRELAIQASKLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRV- 154
           +  +V +G  D +H+GH++L     ++G  + LS +    V+ +EP+     + +   V 
Sbjct: 15  SNSVVTIGNFDGIHLGHQKLFSHIYQIGQKYKLSTI----VVLFEPQPLEFLRKNNAPVR 74

Query: 155 LSSWAPFCRNMAPSEYQ----IQFS-SVRHLTPREFVVK-LSNELGVRGVVAGESYRFGY 214
           ++ +    R ++   +     ++F+ S + L+ ++F++  L N+L ++ +V G  +RFG+
Sbjct: 75  ITKFREKIRRISSYNFDSILCVKFNKSFQSLSAKDFIINILINKLHLKFIVIGNDFRFGF 134

Query: 215 KAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEATNSTNSKERGQVSSTRVRYALSTGDMK 274
           +  G+   L KL  +Y      I+ +   N             ++SST +R ALS  ++K
Sbjct: 135 QRNGNINLLKKLGYKYQFNVIKIRPLYKNNI------------KISSTNIRKALSENNIK 186

Query: 275 YVSELLGR 276
             S LLGR
Sbjct: 195 LASLLLGR 186

BLAST of Csor.00g300690 vs. NCBI nr
Match: KAG6573753.1 (FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 741 bits (1912), Expect = 8.47e-270
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60
           MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG
Sbjct: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60

Query: 61  YSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASK 120
           YSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASK
Sbjct: 61  YSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASK 120

Query: 121 LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180
           LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT
Sbjct: 121 LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180

Query: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEA 240
           PREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEA
Sbjct: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEA 240

Query: 241 TNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPR 300
           TNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPR
Sbjct: 241 TNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPR 300

Query: 301 SCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 360
           SCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE
Sbjct: 301 SCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 360

Query: 361 FDDEGV 366
           FDDEGV
Sbjct: 361 FDDEGV 366

BLAST of Csor.00g300690 vs. NCBI nr
Match: XP_022945275.1 (FAD synthetase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 692 bits (1787), Expect = 7.37e-251
Identity = 347/366 (94.81%), Postives = 349/366 (95.36%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60
           MLAAGA VSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG
Sbjct: 1   MLAAGARVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60

Query: 61  YSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASK 120
           YSFFCSIVPS AAGEPPILSDCFGYRGGGR VSVTGGIVALGK DALHVGHRELAIQASK
Sbjct: 61  YSFFCSIVPSFAAGEPPILSDCFGYRGGGRVVSVTGGIVALGKFDALHVGHRELAIQASK 120

Query: 121 LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180
           LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT
Sbjct: 121 LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180

Query: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEA 240
           PREFVVKLSNELGVRGVVAGESYRFGYKAAGD AE       YGVGAYIIKSVMDKNQE 
Sbjct: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDTAE-------YGVGAYIIKSVMDKNQEF 240

Query: 241 TNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPR 300
           T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSNSRVSVPR
Sbjct: 241 TDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSNSRVSVPR 300

Query: 301 SCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 360
           SCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGTDDYISVE
Sbjct: 301 SCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 359

Query: 361 FDDEGV 366
           FDDEGV
Sbjct: 361 FDDEGV 359

BLAST of Csor.00g300690 vs. NCBI nr
Match: XP_022945276.1 (FAD synthetase 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 585 bits (1507), Expect = 7.39e-209
Identity = 303/366 (82.79%), Postives = 308/366 (84.15%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60
           MLAAGA VSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG
Sbjct: 1   MLAAGARVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60

Query: 61  YSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASK 120
           YSFFCSIVPS AAGEPPILSDCFGYRGGGR VSVT                    + ++ 
Sbjct: 61  YSFFCSIVPSFAAGEPPILSDCFGYRGGGRVVSVT--------------------VNSN- 120

Query: 121 LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180
                               RAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT
Sbjct: 121 --------------------RAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180

Query: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEA 240
           PREFVVKLSNELGVRGVVAGESYRFGYKAAGD AE       YGVGAYIIKSVMDKNQE 
Sbjct: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDTAE-------YGVGAYIIKSVMDKNQEF 240

Query: 241 TNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPR 300
           T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSNSRVSVPR
Sbjct: 241 TDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSNSRVSVPR 300

Query: 301 SCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 360
           SCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGTDDYISVE
Sbjct: 301 SCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 318

Query: 361 FDDEGV 366
           FDDEGV
Sbjct: 361 FDDEGV 318

BLAST of Csor.00g300690 vs. NCBI nr
Match: XP_038891320.1 (FAD synthetase 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 572 bits (1473), Expect = 5.84e-203
Identity = 291/367 (79.29%), Postives = 319/367 (86.92%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSS-PSISHRSQRR 60
           MLAAGA  SHHLRECD HF F   SGL   +SSAV+ FP IWPR PTS+ PSISHRSQRR
Sbjct: 1   MLAAGARASHHLRECDSHFVFGSTSGL---TSSAVLLFPSIWPRYPTSNFPSISHRSQRR 60

Query: 61  GYSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQAS 120
             SFFCSIVPST++ E P+LS+CFG R   REVSV GGIVALGK DALH+GHRELAIQAS
Sbjct: 61  RVSFFCSIVPSTSSDEIPVLSNCFGSREDDREVSVAGGIVALGKFDALHIGHRELAIQAS 120

Query: 121 KLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHL 180
            +GSPFLLSF+GIA+VLGWEPRAP+VAQCDR RVLSSWAP+CRN APSEYQIQFSSVR+L
Sbjct: 121 TVGSPFLLSFIGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYL 180

Query: 181 TPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQE 240
           TPREFV KL+ ELGV GVVAGESYRFGYKAAGDAAELVKLC EYG+GAYIIKSVMD+NQ+
Sbjct: 181 TPREFVEKLTKELGVCGVVAGESYRFGYKAAGDAAELVKLCEEYGIGAYIIKSVMDRNQK 240

Query: 241 ATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVP 300
             NS NSKERGQVSSTRVRYALS GDMKYVSELLGRRHRL+LMAK +EG S SNSRVS P
Sbjct: 241 VVNSANSKERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGFSNSNSRVSAP 300

Query: 301 RSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISV 360
           RSC LNLAPKEGLY+KCFV + D+NLIPCRVVIDS+HV IE+D++ T RH VGT DYI+V
Sbjct: 301 RSCLLNLAPKEGLYNKCFVCTSDENLIPCRVVIDSTHVQIEMDDIGT-RHLVGTQDYINV 360

Query: 361 EFDDEGV 366
           EF DEGV
Sbjct: 361 EFGDEGV 363

BLAST of Csor.00g300690 vs. NCBI nr
Match: XP_023524438.1 (FAD synthetase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570 bits (1470), Expect = 1.80e-202
Identity = 293/368 (79.62%), Postives = 319/368 (86.68%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSS-PSISHRSQRR 60
           MLAAGA VSHHLRECD  FGF L SGL   +S  VV FPPIW RKPTS+ PSIS RSQ R
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGL---TSFPVVLFPPIWSRKPTSNFPSISRRSQPR 60

Query: 61  GYSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQAS 120
           G SFFCSI+PST+ GE P++SD FG R   REVSV GGIVALGK DALHVGHRELAIQAS
Sbjct: 61  GVSFFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS 120

Query: 121 KLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHL 180
            +GSPFLLSFVGIA+VLGWEPRAP+VAQCDR RVLSSWAP+CRN APSEYQIQFSSVR+L
Sbjct: 121 TVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYL 180

Query: 181 TPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQE 240
           TPREFV KLS ELGVRGVVAGESYRFGYKAAGDAAELVKLC EYG+GAYIIKSVMD NQ+
Sbjct: 181 TPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDTNQK 240

Query: 241 ATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNS--RVS 300
             NSTNS ERGQVSSTRVRYALS GDMKYVSELLGRRHRL+LMAK +EGLS SNS  RVS
Sbjct: 241 VVNSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRVS 300

Query: 301 VPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYI 360
            P+SC LNLAPKEGLY+KCF+ + D +LIPCRVVIDS+HVHIE+D++ T RHPVGT +Y+
Sbjct: 301 APKSCLLNLAPKEGLYNKCFICTSDDDLIPCRVVIDSTHVHIEMDDIGT-RHPVGTQNYL 360

Query: 361 SVEFDDEG 365
           SVEFD EG
Sbjct: 361 SVEFDHEG 364

BLAST of Csor.00g300690 vs. ExPASy TrEMBL
Match: A0A6J1G0H2 (FAD synthetase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449571 PE=4 SV=1)

HSP 1 Score: 692 bits (1787), Expect = 3.57e-251
Identity = 347/366 (94.81%), Postives = 349/366 (95.36%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60
           MLAAGA VSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG
Sbjct: 1   MLAAGARVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60

Query: 61  YSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASK 120
           YSFFCSIVPS AAGEPPILSDCFGYRGGGR VSVTGGIVALGK DALHVGHRELAIQASK
Sbjct: 61  YSFFCSIVPSFAAGEPPILSDCFGYRGGGRVVSVTGGIVALGKFDALHVGHRELAIQASK 120

Query: 121 LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180
           LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT
Sbjct: 121 LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180

Query: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEA 240
           PREFVVKLSNELGVRGVVAGESYRFGYKAAGD AE       YGVGAYIIKSVMDKNQE 
Sbjct: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDTAE-------YGVGAYIIKSVMDKNQEF 240

Query: 241 TNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPR 300
           T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSNSRVSVPR
Sbjct: 241 TDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSNSRVSVPR 300

Query: 301 SCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 360
           SCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGTDDYISVE
Sbjct: 301 SCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 359

Query: 361 FDDEGV 366
           FDDEGV
Sbjct: 361 FDDEGV 359

BLAST of Csor.00g300690 vs. ExPASy TrEMBL
Match: A0A6J1G0E4 (FAD synthetase 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449571 PE=4 SV=1)

HSP 1 Score: 585 bits (1507), Expect = 3.58e-209
Identity = 303/366 (82.79%), Postives = 308/366 (84.15%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60
           MLAAGA VSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG
Sbjct: 1   MLAAGARVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60

Query: 61  YSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQASK 120
           YSFFCSIVPS AAGEPPILSDCFGYRGGGR VSVT                    + ++ 
Sbjct: 61  YSFFCSIVPSFAAGEPPILSDCFGYRGGGRVVSVT--------------------VNSN- 120

Query: 121 LGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180
                               RAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT
Sbjct: 121 --------------------RAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLT 180

Query: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEA 240
           PREFVVKLSNELGVRGVVAGESYRFGYKAAGD AE       YGVGAYIIKSVMDKNQE 
Sbjct: 181 PREFVVKLSNELGVRGVVAGESYRFGYKAAGDTAE-------YGVGAYIIKSVMDKNQEF 240

Query: 241 TNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPR 300
           T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSNSRVSVPR
Sbjct: 241 TDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSNSRVSVPR 300

Query: 301 SCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 360
           SCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGTDDYISVE
Sbjct: 301 SCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGTDDYISVE 318

Query: 361 FDDEGV 366
           FDDEGV
Sbjct: 361 FDDEGV 318

BLAST of Csor.00g300690 vs. ExPASy TrEMBL
Match: A0A6J1CF71 (FAD synthase OS=Momordica charantia OX=3673 GN=LOC111010190 PE=4 SV=1)

HSP 1 Score: 570 bits (1470), Expect = 9.03e-203
Identity = 291/368 (79.08%), Postives = 313/368 (85.05%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISH----RS 60
           MLAAGA VSHHLR+CD +FGF L+SG    SSSAVV  PPIWPR PTS PSISH    R 
Sbjct: 1   MLAAGARVSHHLRDCDYYFGFGLSSGF---SSSAVVLLPPIWPRNPTSIPSISHTISPRP 60

Query: 61  QRRGYSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAI 120
            RRG SFFCSIVPST+ GE P+ SDCFG R   REVSV GGIVALGK DALHVGHRELAI
Sbjct: 61  HRRGISFFCSIVPSTSPGEIPLFSDCFGPREDDREVSVAGGIVALGKFDALHVGHRELAI 120

Query: 121 QASKLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSV 180
           QASK+GSPFLLSF GIA+VLGWEPRAPVVAQCDR RVLSSWAP+CRN APSEYQI+FSSV
Sbjct: 121 QASKVGSPFLLSFAGIAEVLGWEPRAPVVAQCDRKRVLSSWAPYCRNSAPSEYQIEFSSV 180

Query: 181 RHLTPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDK 240
           RHLTPREFV KLS ELGVRGVVAGESYRFGYKAAGDA ELVKLC EYG+GA IIKSVMDK
Sbjct: 181 RHLTPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAVELVKLCEEYGLGACIIKSVMDK 240

Query: 241 NQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRV 300
           NQ+  NS NSKERGQVSSTRVRYALS GDMKYVSELLGRRHRL+LMAK +EG S  NSRV
Sbjct: 241 NQKVVNSANSKERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGFSNRNSRV 300

Query: 301 SVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDY 360
           S P+SC LNLAPKEGLY+ CFV + D+NLIPC VVIDS+HVHIE+DE+ TC  P+ T DY
Sbjct: 301 SAPKSCLLNLAPKEGLYNNCFVCTIDENLIPCCVVIDSTHVHIEMDEIGTCN-PLETQDY 360

Query: 361 ISVEFDDE 364
           +SVEF DE
Sbjct: 361 LSVEFGDE 364

BLAST of Csor.00g300690 vs. ExPASy TrEMBL
Match: A0A6J1GWB8 (FAD synthase OS=Cucurbita moschata OX=3662 GN=LOC111458123 PE=4 SV=1)

HSP 1 Score: 570 bits (1468), Expect = 1.52e-202
Identity = 292/366 (79.78%), Postives = 319/366 (87.16%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSS-PSISHRSQRR 60
           MLAAGA VSHHLRECD  FGF L SGL   +S  VV FPPIW RKPTS+ PSIS RSQ R
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGL---TSFPVVLFPPIWSRKPTSNFPSISRRSQPR 60

Query: 61  GYSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQAS 120
           G SFFCSI+PST+ GE P++SD FG R   REVSV GGIVALGK DALHVGHRELAIQAS
Sbjct: 61  GVSFFCSIIPSTSPGEIPVISDSFGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS 120

Query: 121 KLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHL 180
            +GSPFLLSFVGIA+VLGWEPRAP+VAQCDR RVLSSWAP+CRN APSEYQIQFSSVR+L
Sbjct: 121 TVGSPFLLSFVGIAEVLGWEPRAPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYL 180

Query: 181 TPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQE 240
           TPREFV KLS ELGVRGVVAGESYRFGYKAAGDAAELVKLC EYG+GAYIIKSVMD+NQ+
Sbjct: 181 TPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQK 240

Query: 241 ATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVP 300
             NSTNS ERGQVSSTRVRYALS GDMKYVSELLGRRHRL+LMAK +EGLS  NSRVS P
Sbjct: 241 VVNSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLS--NSRVSAP 300

Query: 301 RSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISV 360
           +SC LNLAPKEGLY+ CFV + D++LIPCRVVIDS+HVHIE+D++ T RHPVGT +Y+SV
Sbjct: 301 KSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGT-RHPVGTQNYLSV 360

Query: 361 EFDDEG 365
           EFD EG
Sbjct: 361 EFDHEG 360

BLAST of Csor.00g300690 vs. ExPASy TrEMBL
Match: A0A6J1KGG1 (FAD synthase OS=Cucurbita maxima OX=3661 GN=LOC111493664 PE=4 SV=1)

HSP 1 Score: 566 bits (1459), Expect = 4.11e-201
Identity = 291/368 (79.08%), Postives = 318/368 (86.41%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSS-PSISHRSQRR 60
           MLAAGA VSHHLRECD  FGF L SGL   +S  VV FPPIW RKPTS+ PSIS RSQ R
Sbjct: 1   MLAAGARVSHHLRECDSQFGFGLTSGL---TSFPVVLFPPIWSRKPTSNFPSISRRSQPR 60

Query: 61  GYSFFCSIVPSTAAGEPPILSDCFGYRGGGREVSVTGGIVALGKSDALHVGHRELAIQAS 120
           G SFFCSI+PST+ GE P++SD  G R   REVSV GGIVALGK DALHVGHRELAIQAS
Sbjct: 61  GVSFFCSIIPSTSPGEIPVISDSVGSREDDREVSVAGGIVALGKFDALHVGHRELAIQAS 120

Query: 121 KLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHL 180
            +GSPFLLSFVGIA+VLGWEPR P+VAQCDR RVLSSWAP+CRN APSEYQIQFSSVR+L
Sbjct: 121 TVGSPFLLSFVGIAEVLGWEPRPPIVAQCDRKRVLSSWAPYCRNSAPSEYQIQFSSVRYL 180

Query: 181 TPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQE 240
           TPREFV KLS ELGVRGVVAGESYRFGYKAAGDAAELVKLC EYG+GAYIIKSVMD+NQ+
Sbjct: 181 TPREFVEKLSKELGVRGVVAGESYRFGYKAAGDAAELVKLCEEYGLGAYIIKSVMDRNQK 240

Query: 241 ATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNS--RVS 300
             NSTNS ERGQVSSTRVRYALS GDMKYVSELLGRRHRL+LMAK +EGLS SNS  R+S
Sbjct: 241 VVNSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSNSNSRLS 300

Query: 301 VPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYI 360
            P+SC LNLAPKEGLY+KCFV + D +LIPCRVVIDS+HVHIE+D++ T RHPVGT +Y+
Sbjct: 301 APKSCLLNLAPKEGLYNKCFVCTSDDDLIPCRVVIDSTHVHIEMDDIGT-RHPVGTQNYL 360

Query: 361 SVEFDDEG 365
           SVEFD EG
Sbjct: 361 SVEFDHEG 364

BLAST of Csor.00g300690 vs. TAIR 10
Match: AT5G08340.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 340.9 bits (873), Expect = 1.3e-93
Identity = 189/372 (50.81%), Postives = 248/372 (66.67%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60
           ML  G+ V  HL + + H    L  G      + +V     + R P+ + + SH   R+ 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGF---CGAKIVQLSSFFLR-PSQAMAKSHHFSRKL 60

Query: 61  YSFFCSIVPS--TAAGEPPILSDCFGYRGGGREV------SVTGGIVALGKSDALHVGHR 120
                S   S    +GE PIL +CF  R    E+       V+GGIVALGK DALH+GHR
Sbjct: 61  RQRMISSFGSHCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHR 120

Query: 121 ELAIQASKLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQ 180
           EL IQAS++G+P+LLSFVG+A+VLGWEPRAP+VA+CDR RVL+SWA +C + AP EY+I+
Sbjct: 121 ELTIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIE 180

Query: 181 FSSVRHLTPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKS 240
           F+SVRHLTPR+FV KLS EL V GVVAGE+YRFGYKA+GDA+ELV+LC E G+ A II S
Sbjct: 181 FASVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINS 240

Query: 241 VMDKNQEAT--NSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLS 300
           VMD  Q +   +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRL+L  +  +   
Sbjct: 241 VMDMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--M 300

Query: 301 YSNSRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHP 360
            S   +SVPRS  LNL P  G+Y  C +   D++ +PC VV+D+S++H+E +E+  C   
Sbjct: 301 PSERMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEEVRLCNLD 360

Query: 361 VGTD-DYISVEF 362
              +   +SVEF
Sbjct: 361 WSQEFRLVSVEF 366

BLAST of Csor.00g300690 vs. TAIR 10
Match: AT5G08340.2 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.4e-92
Identity = 184/353 (52.12%), Postives = 240/353 (67.99%), Query Frame = 0

Query: 1   MLAAGAHVSHHLRECDCHFGFRLNSGLPSSSSSAVVPFPPIWPRKPTSSPSISHRSQRRG 60
           ML  G+ V  HL + + H    L  G      + +V     + R P+ + + SH   R+ 
Sbjct: 1   MLCGGSRVLQHLSDHNHHNSIGLGLGF---CGAKIVQLSSFFLR-PSQAMAKSHHFSRKL 60

Query: 61  YSFFCSIVPS--TAAGEPPILSDCFGYRGGGREV------SVTGGIVALGKSDALHVGHR 120
                S   S    +GE PIL +CF  R    E+       V+GGIVALGK DALH+GHR
Sbjct: 61  RQRMISSFGSHCRTSGEVPILHNCFSQREDDPELPVEGLSPVSGGIVALGKFDALHIGHR 120

Query: 121 ELAIQASKLGSPFLLSFVGIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQ 180
           EL IQAS++G+P+LLSFVG+A+VLGWEPRAP+VA+CDR RVL+SWA +C + AP EY+I+
Sbjct: 121 ELTIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLTSWASYCGDRAPEEYEIE 180

Query: 181 FSSVRHLTPREFVVKLSNELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKS 240
           F+SVRHLTPR+FV KLS EL V GVVAGE+YRFGYKA+GDA+ELV+LC E G+ A II S
Sbjct: 181 FASVRHLTPRQFVEKLSKELRVCGVVAGENYRFGYKASGDASELVRLCEECGITACIINS 240

Query: 241 VMDKNQEAT--NSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLS 300
           VMD  Q +   +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRL+L  +  +   
Sbjct: 241 VMDMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--M 300

Query: 301 YSNSRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDE 344
            S   +SVPRS  LNL P  G+Y  C +   D++ +PC VV+D+S++H+E +E
Sbjct: 301 PSERMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEE 347

BLAST of Csor.00g300690 vs. TAIR 10
Match: AT5G23330.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 316.6 bits (810), Expect = 2.6e-86
Identity = 157/277 (56.68%), Postives = 212/277 (76.53%), Query Frame = 0

Query: 77  PILSDCFGYRGGGREVS------VTGGIVALGKSDALHVGHRELAIQASKLGSPFLLSFV 136
           P   DCF       E+S      V GGIVALGK DALH+GHRELAIQA+++G+P+LLSFV
Sbjct: 59  PAPLDCFSQGDDHPELSAEGLSPVAGGIVALGKFDALHIGHRELAIQAARIGTPYLLSFV 118

Query: 137 GIAQVLGWEPRAPVVAQCDRTRVLSSWAPFCRNMAPSEYQIQFSSVRHLTPREFVVKLSN 196
           G+A+VLGW+PRAP+VA+CDR RVLSSWA +C N+AP E++I+F+SVRHL P++FV KLS 
Sbjct: 119 GLAEVLGWKPRAPIVAKCDRKRVLSSWASYCGNIAPVEFEIEFASVRHLNPQQFVEKLSR 178

Query: 197 ELGVRGVVAGESYRFGYKAAGDAAELVKLCNEYGVGAYIIKSVMDKNQEATNS----TNS 256
           EL V GVVAGE+YRFGY+A+GDA+ELV+LC ++G+ AYII SVMDKNQ + N+    + S
Sbjct: 179 ELRVCGVVAGENYRFGYRASGDASELVRLCKDFGISAYIINSVMDKNQVSVNTEEEDSKS 238

Query: 257 KERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPRSCSLNL 316
           KERGQVSSTRVR+AL+ GD++YV+ELLGR HR++   +  + L+    R+S+  S  LNL
Sbjct: 239 KERGQVSSTRVRHALAAGDVRYVTELLGRPHRVISRTR-TQDLTSKRGRISLQTSSLLNL 298

Query: 317 APKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDE 344
            P  G+Y  C +   D++ I C+V++D+S+++IE +E
Sbjct: 299 PPGNGVYKACSLIVGDKHPISCKVIVDTSNLYIETEE 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZR01.8e-9250.81FAD synthetase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF2 PE=1 SV... [more]
Q9FMW83.6e-8556.68FAD synthetase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF1 PE=1 SV... [more]
Q10BX93.1e-8455.56FAD synthetase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBF P... [more]
P572501.4e-0725.00Bifunctional riboflavin kinase/FMN adenylyltransferase OS=Buchnera aphidicola su... [more]
Match NameE-valueIdentityDescription
KAG6573753.18.47e-270100.00FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022945275.17.37e-25194.81FAD synthetase 2, chloroplastic-like isoform X1 [Cucurbita moschata][more]
XP_022945276.17.39e-20982.79FAD synthetase 2, chloroplastic-like isoform X2 [Cucurbita moschata][more]
XP_038891320.15.84e-20379.29FAD synthetase 2, chloroplastic-like [Benincasa hispida][more]
XP_023524438.11.80e-20279.62FAD synthetase 2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1G0H23.57e-25194.81FAD synthetase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1G0E43.58e-20982.79FAD synthetase 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1CF719.03e-20379.08FAD synthase OS=Momordica charantia OX=3673 GN=LOC111010190 PE=4 SV=1[more]
A0A6J1GWB81.52e-20279.78FAD synthase OS=Cucurbita moschata OX=3662 GN=LOC111458123 PE=4 SV=1[more]
A0A6J1KGG14.11e-20179.08FAD synthase OS=Cucurbita maxima OX=3661 GN=LOC111493664 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08340.11.3e-9350.81Nucleotidylyl transferase superfamily protein [more]
AT5G08340.22.4e-9252.12Nucleotidylyl transferase superfamily protein [more]
AT5G23330.12.6e-8656.68Nucleotidylyl transferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 90..276
e-value: 4.3E-32
score: 113.2
IPR015864FAD synthetasePFAMPF06574FAD_syncoord: 175..243
e-value: 9.7E-9
score: 35.3
NoneNo IPR availablePANTHERPTHR12714:SF20FAD SYNTHETASE 1, CHLOROPLASTIC-RELATEDcoord: 46..362
NoneNo IPR availablePANTHERPTHR12714PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASEcoord: 46..362
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 99..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g300690.m01Csor.00g300690.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006747 FAD biosynthetic process
biological_process GO:0009231 riboflavin biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0003919 FMN adenylyltransferase activity